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Gut microbiota can influence host gene expression and physiology through metabolites. Besides, the presence or absence of gut microbiome can reprogram host transcriptome and epitranscriptome as represented by N6-methyladenosine (m6A), the most abundant mammalian mRNA modification. However, which and how gut microbiota-derived metabolites reprogram host transcriptome and m6A epitranscriptome remain poorly understood. Here, investigation is conducted into how gut microbiota-derived metabolites impact host transcriptome and m6A epitranscriptome using multiple mouse models and multi-omics approaches. Various antibiotics-induced dysbiotic mice are established, followed by fecal microbiota transplantation (FMT) into germ-free mice, and the results show that bile acid metabolism is significantly altered along with the abundance change in bile acid-producing microbiota. Unbalanced gut microbiota and bile acids drastically change the host transcriptome and the m6A epitranscriptome in multiple tissues. Mechanistically, the expression of m6A writer proteins is regulated in animals treated with antibiotics and in cultured cells treated with bile acids, indicating a direct link between bile acid metabolism and m6A biology. Collectively, these results demonstrate that antibiotic-induced gut dysbiosis regulates the landscape of host transcriptome and m6A epitranscriptome via bile acid metabolism pathway. This work provides novel insights into the interplay between microbial metabolites and host gene expression.

The 21st century is the century of life science. With the rapid development of life science and technology, the social needs of biological professionals are changing dramatically, and the requirements for their theoretical quality and practical ability are becoming higher and higher. The construction of characteristic disciplines in Henan Province is advancing steadily, which is having a far-reaching impact on the talent cultivation of colleges and universities in the province, and the talent training mode of colleges and universities has also changed greatly. With the continuous expansion of the national undergraduate enrollment scale, it is of great theoretical and practical significance for local normal universities to cultivate biological professionals to adapt to the development of life science and social needs, and to explore a new model for the cultivation of biological professionals. Xinyang Normal University, based on the experience of biological talents training in the past 45 years, especially in the past five years around the construction of Henan Province's characteristic disciplines, focuses on the construction of "teaching staff, teaching team and practice team", focusing on improving the theoretical quality and practical ability of students majoring in biology, that is, a new training model of "one center, two improvements" for biological professionals. The new model has important theoretical significance and potential application value for the continuous improvement of teaching quality of biology education.

In women, ovarian cancer is a common gynecological cancer associated with poor prognosis, reoccurrence and chemoresistance. Scoulerine, a benzylisoquinoline alkaloid, has been reported effective against several carcinomas. Thus, we investigated the impact of scoulerine on ovarian cancer cells (OVCAR3). Cell viability was assessed by MTT assay, migration was determined by Boyden Chamber assay, while the invasion was monitored by Boyden Chamber assay using the matrigel. The stemness properties of OVCAR3 cells were observed by tumorsphere assay. Epithelial to mesenchymal transition (EMT) and stemness-related protein markers were monitored by real-time PCR analysis and immunoblotting. Scoulerine inhibits the viability of OVCAR3 cells with the IC50 observed at 10 µmol L–1 after 48 h treatment. Scoulerine inhibited the colony-forming ability, migration and invasiveness of OVCAR3 cells in a dose-dependent fashion. Scoulerine treatment also drastically reduced the spheroid-forming ability of OVCAR3 cells. The mesenchymal and stemness--related markers like N-cadherin, vimentin, CD-44, Oct-4, Sox-2 and Aldh1A1 were downregulated, whereas the epithelial markers like E-cadherin and CD-24 were upregulated in scoulerine-treated cells. The upstream PI3K/Akt/mTOR-axis was downregulated in scoulerine-treated cells. We concluded that scoulerine successfully perturbs the cancerous properties of OVCAR3 cells by targeting the PI3K/Akt/mTOR axis. In vivo studies revealed a substantial decrease in tumor mass and volume after scoulerine treatment. Furthermore, scoulerine treatment was found to decrease oxidative stress factors in ovarian cancer mice model. Scoulerine is a potential anticancer agent against ovarian cancer and can be considered as a lead molecule for this malignancy, provided further investigations are performed.

In order to effectively investigate the current situation of biology teachers in Southern Henan, this study used the method of interviews and questionnaires to investigate and analyze the current situation of biology teachers in Southern Henan from their living status and professional development. The survey results show that: (1) The overall satisfaction of biology special post teachers in southern Henan is general, the office conditions can meet the teaching needs, the salary and housing conditions need to be improved, and the spare-time and family life needs of biology special post teachers attract attention; (2) Professional development is generally satisfactory. Specialized biology teachers in Southern Henan are willing to participate in educational and teaching reform, but their participation in teaching and research activities needs to be further strengthened. Specialized biology teachers have a large workload and high labor intensity. In view of the above findings, this paper puts forward some countermeasures for the development of biology special post teachers in Southern Henan, with a view to providing theoretical reference for the follow-up research on biology special post teachers, as well as providing important information for improving the living status of specialty post teachers, promoting professional development and improving the quality of education and teaching.

Objective To investigate the effect of lncRNA SNHG14 targeting at miR-142-5p on the neuronal damage induced by oligomeric Aβ. Methods PC12 cells were divided into control group, model group, model+si-NC group, model+si-SNHG14 group, model+miR-NC group, model+miR-142-5p group, model+si-SNHG14+anti-miR-NC group, and model+ si-SNHG14+anti-miR-142-5p group. The level of SNHG14 and miR-142-5p was calculated by RT-qPCR. CCK-8 method and flow cytometry were applied to detect inhibition rate on cell proliferation and apoptosis rate. ELISA method was used to detect TNF-αand IL-6 in the culture. Dual luciferase report assay was used to find the targeting relationship between SNHG14 and miR-142-5p. Results SNHG14 was highly expressed (P＜0.05)while miR-142-5p was lowly expressed (P＜0.05) in plasma of AD patients. Compared with the control group, the SNHG14 level, cell proliferation inhibition rate, and apoptosis rate in the model group were significantly increased (P＜0.05), the level of miR-142-5p was significantly reduced (P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P＜0.05), IL-10 level was significantly decreased (P＜0.05). Compared with the model+si-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+si-SNHG14 group were significantly reduced (P＜0.05), the level of TNF-α and IL-6 in the culture medium was significantly reduced (P＜0.05), IL-10 level was significantly increased(P＜0.05). Compared with the model+miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+miR-142-5p group were significantly reduced (P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly reduced (P＜0.05) and IL-10 level was significantly increased (P＜0.05). miR-142-5p was a direct target of SNHG14. Compared with the model+si-SNHG14+ anti-miR-NC group, the cell proliferation inhibition rate and apoptosis rate in the model+ si-SNHG14+anti-miR-142-5p group were significantly increased(P＜0.05), and the levels of TNF-α and IL-6 in the culture medium were significantly increased (P＜0.05), IL-10 level was significantly decreased (P＜0.05). Conclusions Silencing SNHG14 may alleviate oligomeric Aβ-induced neuronal damage by targeting at and promoting miR-142-5p expression.

The application of finite control set model predictive control (FCS-MPC) in six-phase permanent magnet synchronous motors (PMSMs) often faces a trade-off between computational burden and accurate voltage vector selection, as well as challenges related to harmonic components and torque generation. This paper introduces an improved model predictive current control (MPCC) method to address these problems. Firstly, 12 virtual voltage vectors are synthesized to improve torque output performance while suppressing harmonic currents. Then, to generate symmetrical switching signals and reduce switching loss, the largest basic vector used to synthesize the virtual vector is replaced by two medium vectors. Secondly, to solve the problem of the increased computational burden caused by the increase in discrete virtual vectors, a two-step vector selection method is proposed. In this method, each part is divided into several parts according to N, and the traditional cost function is also replaced by two-step functions. Different control performances can be achieved according to different values of N. Experimental results show that the proposed control scheme not only achieves stable current quality but also significantly improves steady-state performance throughout the entire speed range. [ABSTRACT FROM AUTHOR]