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ABSTRACT: Aim: In this in vitro study, the microleakage evaluation of repaired pit and fissure sealants with various surface treatments was compared. Study Design: The study included 120 extracted human third molars. The teeth were divided into two groups of 60 each. Group 1 was sealed with Clinpro™ unfilled sealant, while group 2 was sealed with Grandioseal nanofilled sealants. After curing, half of the polymerized sealant were removed. Both groups were divided further into two subgroups of 30 teeth each, with repair techniques using either Adper Easy One or Futura Bond. Three sections were obtained from each tooth resulting 6 surfaces per tooth to be examined and the sections of all four groups were scored for microleakage. Results: Conventional acid etching was found to be superior to self-etch adhesive technique. Conclusion: Microleakage was found to be greater with Grandio seal compared to Clinpro. [ABSTRACT FROM AUTHOR]

ABSTRACT: Background: The use of SHED might bring advantages for tissue engineering over the use of stem cells from adult human teeth as SHEDs were reported to have higher proliferation rate and increase cell population doublings as compared with stem cells from permanent teeth. Objective: The study's objective was to assess the growth kinetics and tri-lineage differentiation capacity of stem cells derived from human exfoliated deciduous teeth. Material and Method: After being propagated through multiple sequential subcultures, adherent fibroblastic cells in the pulp tissue culture from human exfoliation teeth were evaluated to assess their tripotent differentiation potential into bone, cartilage, and adipose cell lineages. Result: The stem cells derived from dental pulp had a comparatively high ability for proliferation and successfully differentiated into chondrocytes and osteocytes while less significant for adipocytes. Conclusion: Stem cells from Human Exfoliated Deciduous teeth are mesenchymal stem cells which are multipotent and can serve as a promising incentive for therapeutic and future research purposes. [ABSTRACT FROM AUTHOR]

Oral squamous papillomas are benign proliferating lesions induced by human papillomavirus. These lesions are painless and slowly-growing masses. As an oral lesion, it raises concern because of its clinical appearance. These lesions commonly occur between age 30 and 50 years, and sometimes can occur before the age of 10 years. Oral squamous papilloma accounts for 8% of all oral tumors in children. Common site predilection for the lesion is the tongue and palate and may occur on any other surface of the oral cavity such as the uvula and vermilion of the lip. Here, we are presenting a case of squamous papilloma on the palate in a 9-year-old child.

H1N1 influenza, also known as "novel H1N1 virus" has led to a "global outcry." This virus is more virulent when compared with other seasonal flu viruses. Virulence may change as the adaptive mutation gene increases within the virus. A study at the US Centre for Disease Control and Prevention published in May 2009 found that children had no preexisting immunity to the new strain as they showed no cross-reactive antibody reaction when compared with adults aged 18-64 years, who showed a cross-reactive antibody reaction of 6-9% and older adults with 33% immunity. This review article depicts H1N1 virus, its virulence with genetic evolution potential and preventive protocol for the dental professionals. This would allow us to comprehend the changes in the disease process and contribute in its prevention as "prevention is better than cure."

Children are uniquely susceptible to cranio facial trauma because of their greater cranial mass to body ratio. Below the age of 5, the incidence of pediatric facial fractures in relation to the total is very low ranging from 0.6-1.2%. Maxillo-facial injuries may be quite dramatic causing parents to panic and the child to cry uncontrollably with blood, tooth and soft tissue debris in the mouth. The facial disfigurement caused by trauma can have a deep psychological impact on the tender minds of young children and their parents. This case report documents the trauma and follow up care of a 4-year-old patient with maxillofacial injuries.

Background and Objectives: The aciduric mutans streptococci (MS) group, including Streptococcus mutans (SM) and Streptococcus sobrinus (SS), is highly cariogenic. Relationship between increments in dental caries and the presence of these species is not yet clarified. It is of great importance to separately determine the presence of these two species for understanding their role in dental caries, accurate prediction, and effective prevention. Hence, this study was undertaken to detect the presence of SM and SS in plaque samples of caries-free (CF), early childhood caries (ECC), and severe early childhood caries (S-ECC) and also to compare the possible relationship between these species with the occurrence of ECC.Materials and Method: In all, 120 healthy children between 3 and 6 years of age were randomly allocated into three groups: Group A (n = 40) CF, Group B (n = 40) ECC, and Group C (n = 40) S-ECC. Plaque samples were collected from all approximal surface and gingivobuccal surfaces of teeth using a sterile universal scaler and dmft/dmfs scores were taken. The presence of SM and SS was evaluated using real-time polymerase chain reaction.Results: The presence of SM in CF, ECC, and S-ECC was 10.0%, 27.5%, and 42.5%, respectively, and SS was 5.0%, 40.0%, and 47.5% in CF, ECC, and S-ECC, respectively. Comparison of mean relative quantification (RQ) values of SM between three study groups showed significant results between CF and S-ECC at P value 0.003. Comparison of mean RQ values of SS showed significant results between CF to ECC and CF to S-ECC at P value <0.001. This study showed significant results between the mean dmft/dmfs scores in three study groups and the presence of high levels of SM and SS in ECC and S-ECC groups. However, the study showed weak positive correlation between dmfs scores and both SM and SS species in ECC and S-ECC.Interpretations and Conclusion: Higher levels of SS were more closely related to the caries-active lesions leading to severity of dental disease, that is, both in ECC and S-ECC. There was significant difference between mean dmft/dmfs scores in three study groups and it also showed positive correlation between dmfs scores and SM and SS colonization in ECC and S-ECC status. [ABSTRACT FROM AUTHOR]

Background and Objective: Glass ionomer cements (GICs) are the most commonly used restorative material in pediatric dentistry. They have numerous advantages; however, they show some marginal microleakage at the restoration-tooth interface. Various conditioning agents have been tested for alteration or dissolution of smear layer which has been attributed to the occurrence of microleakage; however, very limited studies have been done using primary teeth.Aims: To evaluate and compare the effect of 10% polyacrylic acid and 17% EDTA on marginal microleakage of high-viscosity GIC.Settings and Design: Experimental, in vitro study.Methodology: Class V cavities of standardized dimensions were prepared on 60 primary anterior teeth and were randomly divided into three groups. Except Group I, the cavities of Groups II and III were conditioned with 10% polyacrylic acid and 17% EDTA, respectively. All the 60 teeth were then restored with high-viscosity GIC. The samples were thermocycled and immersed in methylene blue solution for 24 h. The teeth were removed from the stain, rinsed, and sectioned buccolingually and were observed under stereomicroscope at 30× to score the marginal microleakage.Statistical Analysis Used: Kruskal-Wallis test followed by Mann-Whitney post hoc analysis were used to compare the mean marginal leakage scores between the three study groups.Results: Statistical significance difference was found between all the three groups (P < 0.05). The mean marginal microleakage score was maximum for Group I (control group; 3.00), whereas it was the least for Group II (1.30) where 10% polyacrylic was used for conditioning the cavity surface.Conclusion: Among the study groups, none of them was completely devoid of microleakage in all its samples. 10% polyacrylic acid emerged as a better conditioning agent when compared with 17% EDTA in altering or removing the smear layer thereby resulting in better adhesion. [ABSTRACT FROM AUTHOR]

Context: A major goal in pediatric dentistry is preservation of the integrity of primary teeth and their supporting tissues until physiological process of exfoliation takes place. Pulpectomy serves such a purpose using various materials and techniques to fill the canals of primary teeth. Aims: The aim of this in vivo study was to determine the efficacy of modified disposable syringe technique in root canals of primary molars using digital radiography when obturated with endoflas. Settings and Design: A clinical study was undertaken for a period of 6 months. Subjects and Methods: A total of 60 primary maxillary and mandibular molars were selected in the age group of 4-8 years and randomly divided into two groups. The teeth were obturated with handheld lentulospiral and a modified disposable syringe techniques. Postoperative radiographic evaluation was done for quality of fill and voids using digital radiography. Statistical Analysis Used: Results were assessed using Chi-square test and Mann--Whitney U-test. Results: No statistically significant difference between quality of obturation using hand-held lentulospiral and modified disposable syringe (P < 0.05) was observed. Optimal obturation was achieved in both techniques; however, voids in obturation were not significant. Conclusions: Both the hand-held lentulospiral and modified disposable syringe technique are effective in the obturation of primary molar root canals in terms of quality of fill. [ABSTRACT FROM AUTHOR]

SHEDs have been shown to have a higher rate of proliferation and raise in cell population doublings when compared to stem cells from permanent teeth. Hence, using them in tissue engineering may be advantageous over stem cells from adult human teeth. Stem cells were removed from pulpal tissues of thirty primary teeth undergoing extraction under six to fourteen year of age. The tissues were incubated after centrifuging and adding DMEM-KO following the addition of a 2 mg/ml collagenase blend for examination of plates in search of cell attachment and growth. Flow cytometric analysis showed successful isolation of SHEDs using fluoresce inisothiocyanate (FITC)-conjugated CD-34, CD-105, and PE (R-phycoerythrin)-conjugated CD-45, CD-90, CD-73, and HLA-DR antibodies. The surface antigens CD-73, CD-90 and CD-105 which are known to be present in mesenchymal lineages were positively expressed in SHEDs according to flow cytometry analysis, whereas CD-34, CD-45, and HLA-DR were not., (© 2024 Biomedical Informatics.)

Background: Radiography is one of the most valuable diagnostic tools used in comprehensive dental care. Radiation from dental radiographs was thought to cause cytogenetic changes and its plausible effects can remain for some hours, months, or generations especially in children., Aims and Objectives: To evaluate and compare the possible genotoxic effect of routinely used intraoral periapical radiographic exposure and radiovisiographic exposure in exfoliated epithelial cells as measured by the formation of micronuclei during single visit pulpectomy procedure using Buccal Micronucleus Cytome (BMCyt) assay in children., Materials and Methods: Study comprised 60 healthy children who has undergone either intraoral periapical radiography (IOPAR; group 1, n = 30) or radiovisiography (RVG; group 2, n = 30) during various steps of single visit pulpectomy procedure. Cytological smears were taken from the buccal mucosa immediately before the X-ray exposure and 10 ± 2 days after exposure. The cells were stained with Feulgen and evaluated for micronuclei by scoring 1,000 cells per sample., Results: The genotoxic effect of radiation exposure from intraoral periapical radiography higher than that of RVG showing significant increase in micronucleus (MN) formation., Conclusion: The X-ray radiation emitted during IOPAR or RVG does induce genotoxic changes in the form of increased frequency of micronuclei. So, great care and standard protocol should be followed to advice radiographs if necessary and reduce the cumulated biological effects of radiation exposure., Keynote: Taking into account the strong evidence of a relationship between DNA damage and carcinogenesis and the extensive application of intraoral radiographs in pediatric dentistry, it would be useful to know to what extent these dental X-rays cause genotoxic effects resulting in DNA damage on oral mucosa., How to Cite This Article: Julu Y, Nagarathna C. Biomonitoring of Genotoxic Effect in Children Exposed to Dental Radiographs during Pulpectomy Procedure-BMCyt Assay. Int J Clin Pediatr Dent 2022;15(S-1):S63-S70., Competing Interests: Source of support: Nil Conflict of interest: None, (Copyright © 2022; The Author(s).)

Aim: "Today's adolescents are tomorrow's citizens." Tobacco use in children and adolescents is reaching pandemic levels as they are the most vulnerable population to initiate tobacco use. It is well established that most of the adult users of tobacco, start the use of tobacco either in their childhood or adolescence. Parents are the best route to reach a child and can help lead to bring better outcomes for children. Hence, the aim of this study is to assess the knowledge, attitude, and behavior regarding the hazards of tobacco use and Cigarette and Other Tobacco Products Act (COTPA) among the parents visiting pediatric dental clinic., Materials and Methods: A cross-sectional survey was conducted using a self-administered questionnaire regarding knowledge, attitude, and behavior regarding the hazards of tobacco use and COTPA law. Samples size of 400 parents of adolescents aged between 10 and 16 years visiting to the department of Pediatrics and Preventive Dentistry were included in the study, data thus obtained was subjected to statistical analysis., Results: Smoking tobacco product known to the parent population in the present study is cigarette (88.8%) and smokeless tobacco product is pan masala (65.2%). The tobacco health hazard known is cancer by 70.5 and 85.3% parent populations were aware of mandatory display of pictorial health warnings in COTPA law. Strict implementation of COTPA law was opted by 61.8%., Conclusion: Knowledge, attitude, and behavior of parents is required to educate and motivate adolescents. Parents have given their positive attitude regarding the strict implementation of COTPA law in India and realize the role of tobacco as a causative factor for health hazards both in children and adults. COTPA law should be made aware for both young and old to educate and motivate and to prevent the use of tobacco in India., How to Cite This Article: Kattimani S, Thimmegowda U, Nagarathna C. Knowledge, Attitude and Behavior Regarding the Hazards of Tobacco Use and Cigarette and Other Tobacco Products Act among Parents Visiting Pediatric Dental Clinic: A Cross-sectional Questionnaire Survey. Int J Clin Pediatr Dent 2022;15(1):47-53., Competing Interests: Source of support: Nil Conflict of interest: None, (Copyright © 2022; The Author(s).)

Objective: Data on cognitive changes in patients with tuberculous meningitis (TBM) are sparse. We aimed to study the cognitive profiles of patients with grade I TBM and correlate them with the cytokine values., Methods: Prospectively, 60 patients (M:F-31:29) with grade I TBM were recruited. Clinical details were collected; CSF estimation of cytokines, neuropsychological assessment, and correlation were performed., Results: Mean age at presentation was 32.2 years (32.2 ± 10.1), and the duration of symptoms was 29.9 days (29.9 ± 25.9), respectively. Definitive evidence of mycobacterial infection was observed in 28.3% of the patients. Mean levels of tumor necrosis factor-α (TNF-α), interferon (IFN-γ), and interleukin-6 (IL-6) were 11.57 ± 30.35, 197.02 ± 186.64, and 127.03 ± 88.71 pg/mL, respectively. TNF-α levels were significantly elevated in definitive TBM ( p = 0.044). Neuropsychological tests revealed an impaired auditory verbal learning test (88.3%), followed by complex figure test (50%), spatial span test (50%), clock drawing test (48.3%), digit span test (35%), color trail tests 1 and 2 (30% and 33.3%, respectively), and animal naming test (28.3%). Elevated levels of IFN-γ and IL-6 in TBM directly correlated with the number of impaired neuropsychological tests. During follow-up, significant improvement was noticed in animal naming test ( p = 0.005), clock drawing test ( p = 0.003), color trail test 2 (0.02), spatial span test ( p = 0.012), and digit span test (0.035). Verbal learning did not show any significant change. Overall, the neuropsychological tests showed better recovery of attention, working memory, and category fluency and showed minimal recovery of verbal learning., Conclusions: There is subclinical evidence of cognitive impairment in patients with TBM, and this correlated with elevated cytokines. Both the frontal and temporal lobes showed varying degrees of cognitive impairment., (© 2021 American Academy of Neurology.)

Background: In children, stainless steel crowns (SSCs) have become an invaluable restorative option for grossly decayed primary teeth. The crowns are manufactured in different sizes with preformed anatomy which requires trimming or contouring as a necessary step to fit an individual tooth. Initially, this may produce variation in the occlusal contact points yet may not result in pain or discomfort. Little research exists regarding bite force equilibration measurements in children after placing SSC, and its influencing factors such as age and gender., Aim and Objective: To evaluate and compare the measurements of bite force pre- and post-cementation of SSC using the conventional technique at maximal intercuspal position (MIP) on primary molars at different time intervals and whether age and gender influence bite force measurements., Design: Bite force and occlusal contacts during occlusion were made using T-Scan III. Twenty children scheduled for treatment who needed SSCs were included. T-Scan measurements of the bite force and occlusal contacts of the maxilla and mandibular teeth were recorded and analyzed before and immediately after cementation of SSC later 4 weeks during the follow-up period. These measurements were correlated with age and gender influence., Results: There was no significant result in the percentage of bite force on the crowned tooth (placement of SSC) at different time intervals. Prematurities were present in all the groups but a significant reduction was seen on the crowned tooth from baseline to 1-month follow-up ( p = 0.03). Also, a statistically significant increase in the bite force was seen in >7 years of age ( p = 0.006) and no statistical significance among the gender., Conclusion: Following the standard tooth preparation, the SSC will continue to appear clinically acceptable for many years. Our study children showed an adaptable masticatory system irrespective of age and gender during growth and development., How to Cite This Article: Aishwarya N, Nagarathna C, Poovani S, et al. Comparison of Bite Force and the Influencing Factors Pre- and Post-cementation of Stainless Steel Crown in Children Using T-Scan. Int J Clin Pediatr Dent 2021;14(1):46-50., Competing Interests: Source of support: Nil Conflict of interest: None, (Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd.)

Background and Objectives: The aciduric mutans streptococci (MS) group, including Streptococcus mutans (SM) and Streptococcus sobrinus (SS), is highly cariogenic. Relationship between increments in dental caries and the presence of these species is not yet clarified. It is of great importance to separately determine the presence of these two species for understanding their role in dental caries, accurate prediction, and effective prevention. Hence, this study was undertaken to detect the presence of SM and SS in plaque samples of caries-free (CF), early childhood caries (ECC), and severe early childhood caries (S-ECC) and also to compare the possible relationship between these species with the occurrence of ECC., Materials and Method: In all, 120 healthy children between 3 and 6 years of age were randomly allocated into three groups: Group A (n = 40) CF, Group B (n = 40) ECC, and Group C (n = 40) S-ECC. Plaque samples were collected from all approximal surface and gingivobuccal surfaces of teeth using a sterile universal scaler and dmft/dmfs scores were taken. The presence of SM and SS was evaluated using real-time polymerase chain reaction., Results: The presence of SM in CF, ECC, and S-ECC was 10.0%, 27.5%, and 42.5%, respectively, and SS was 5.0%, 40.0%, and 47.5% in CF, ECC, and S-ECC, respectively. Comparison of mean relative quantification (RQ) values of SM between three study groups showed significant results between CF and S-ECC at P value 0.003. Comparison of mean RQ values of SS showed significant results between CF to ECC and CF to S-ECC at P value <0.001. This study showed significant results between the mean dmft/dmfs scores in three study groups and the presence of high levels of SM and SS in ECC and S-ECC groups. However, the study showed weak positive correlation between dmfs scores and both SM and SS species in ECC and S-ECC., Interpretations and Conclusion: Higher levels of SS were more closely related to the caries-active lesions leading to severity of dental disease, that is, both in ECC and S-ECC. There was significant difference between mean dmft/dmfs scores in three study groups and it also showed positive correlation between dmfs scores and SM and SS colonization in ECC and S-ECC status., Competing Interests: None