Search

The aim of the study was to investigate the effect of Leu-Ile-Lys-Met tetrapeptide on the activity of free radical oxidation (FRO) and the level of cyclooxygenase-1 (COX-1) and COX-2 expression in rat kidney with experimental oxalate nephrolithiasis. Material and methods. The experiments were carried out on 30 male rats Wistar runoff, divided into 3 groups: the comparison group (10 intact rats), the control group (6 weeks of experimental oxalate nephrolithiasis simulation, n = 10), the experimental group (6 weeks of experimental simulation oxalate nephrolithiasis + oral administration of Leu-Ile-Lys-Met tetrapeptide at a dose of 12 mg/kg from the 3rd to the 6th week, n = 10). The oxalate nephrolithiasis was simulated according to conventional ethylene glycol model. COX-1 and COX-2 concentration in rat urine at baseline and after 6 weeks of experimental nephrolithiasis was determined by enzyme immunoassay using the prostaglandin-endoperoxide synthase 1 and 2 (PTGS 1, 2) kit from «Cloud-Clone Corp.». In rat kidney homogenate, the activity indicators of FRO processes were determined by common methods. Results and discussion. At the background of the Leu-Ile-Lys-Met tetrapeptide, free radical kidney damage was weakened, which was manifested in the complete absence of morphological signs of nephrolithiasis, decrease of thiobarbituric acid-reactive product concentration and overall prooxidant activity as compared to the control group; glutathione peroxidase activity, on the contrary, increased by 1.5 times.

Background: The research objective was to study the effect of the tetrapeptide Leu-Ile-Lys-His on the course of experimental oxalate nephrolithiasis. Methods and Results: Experiments were conducted on 30 male Wistar rats, which were divided equally into 3 groups: the control group (6 weeks of ON modeling), the test group (6 weeks of ON modeling+the tetrapeptide Leu-Ile-Lys-His introduction at a dose of 12 mg/kg), and the experimental group (determination of indicators of free radical oxidation process activity in intact rats). Every 7 days, indicators of the excretory renal function (the level of diuresis and creatinine excretion) and activity of marker enzymes of renal tissue damage (lactate dehydrogenase and γ-glutamyl transferase) were determined in the urine of rats. After six weeks, we determined parameters of activity of the free radical oxidation process in the kidneys of rats posthumously and conducted the morphological study for formation of calcium deposits. Conclusion: Experiments showed that in the control group there were characteristic signs of nephrolithiasis development: increase of LDH activity by 5.4 times, increase of free radical oxidation process activity in the kidneys, and formation of large concrements in the kidneys, the mean size of which was 298.8±34.2 μm2. In the test group, there was weakening of LDH and GGT activity and weakening of free radical oxidation process activity. There were no morphological signs of nephrolithiasis development in the kidneys.