3 results on '"Moncada, CA"'
Search Results
2. Mechanism and tissue specificity of nicotine-mediated lung S-adenosylmethionine reduction.
- Author
-
Moncada CA, Clarkson A, Perez-Leal O, and Merali S
- Subjects
- Animals, Biogenic Polyamines biosynthesis, Guinea Pigs, Microdissection, Organ Specificity drug effects, Oxidation-Reduction drug effects, Pneumocystis, Pneumonia, Pneumocystis pathology, Pulmonary Alveoli pathology, Rats, Rats, Sprague-Dawley, Respiratory Mucosa enzymology, Time Factors, Gene Expression Regulation, Enzymologic drug effects, Nicotine pharmacology, Nicotinic Agonists pharmacology, Ornithine Decarboxylase biosynthesis, Pneumonia, Pneumocystis enzymology, Pulmonary Alveoli enzymology, S-Adenosylmethionine metabolism
- Abstract
We previously reported that chronic nicotine infusion blocks development of Pneumocystis pneumonia. This discovery developed from our work demonstrating the inability of this fungal pathogen to synthesize the critical metabolic intermediate S-adenosylmethionine and work by others showing nicotine to cause lung-specific reduction of S-adenosylmethionine in guinea pigs. We had found nicotine infusion to cause increased lung ornithine decarboxylase activity (rate-controlling enzyme of polyamine synthesis) and hypothesized that S-adenosylmethionine reduction is driven by up-regulated polyamine biosynthesis. Here we report a critical test of our hypothesis; inhibition of ornithine decarboxylase blocks the effect of nicotine on lung S-adenosylmethionine. Further support is provided by metabolite analyses showing nicotine to cause a strong diversion of S-adenosylmethionine toward polyamine synthesis and away from methylation reactions; these shifts are reversed by inhibition of ornithine decarboxylase. Because the nicotine effect on Pneumocystis is so striking, we considered the possibility of tissue specificity. Using laser capture microdissection, we collected samples of lung alveolar regions (site of infection) and respiratory epithelium for controls. We found nicotine to cause increased ornithine decarboxylase protein in alveolar regions but not airway epithelium; we conclude that tissue specificity likely contributes to the effect of nicotine on Pneumocystis pneumonia. Earlier we reported that the full effect of nicotine requires 3 weeks of treatment, and here we show recovery is symmetrical, also requiring 3 weeks after treatment cessation. Because this time frame is similar to pneumocyte turnover time, the shift in polyamine metabolism may occur as new pneumocytes are produced.
- Published
- 2008
- Full Text
- View/download PDF
3. Effect of nicotine on lung S-adenosylmethionine and development of Pneumocystis pneumonia.
- Author
-
Shivji M, Burger S, Moncada CA, Clarkson AB Jr, and Merali S
- Subjects
- Animals, Electrophoresis, Gel, Two-Dimensional, Gene Expression Profiling, Guinea Pigs, Humans, Image Processing, Computer-Assisted, Inhibitory Concentration 50, Isoelectric Focusing, Kinetics, Liver metabolism, Lung metabolism, Lung microbiology, Methionine Adenosyltransferase metabolism, Nicotine chemistry, Pneumocystis metabolism, Pneumonia, Pneumocystis drug therapy, Polyamines chemistry, Polyamines metabolism, Polymerase Chain Reaction, Proteomics methods, Rats, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Time Factors, Trypsin pharmacology, Up-Regulation, Lung drug effects, Nicotine pharmacology, Pneumonia, Pneumocystis metabolism, S-Adenosylmethionine chemistry
- Abstract
Because S-adenosylmethionine (AdoMet) is required by Pneumocystis carinii in vitro, Pneumocystis infection depletes plasma AdoMet of rats and humans, nicotine reduces AdoMet of guinea pig lungs, and smoking correlates with reduced episodes of Pneumocystis pneumonia (PCP) in AIDS patients, we tested the effect of nicotine treatment on PCP using a rat model. Intraperitoneal infusion of 400 microg of R-(+) nicotine kg(-1) h(-1) intraperitoneal for 21 days caused a 15-fold reduction in lung AdoMet although neither plasma nor liver were changed. Infusion of 4 and 400 microg kg(-1) h(-1) into immunosuppressed rats, beginning when rats were inoculated with P. carinii, caused 85 and 99.88% reductions, respectively, in P. carinii cysts at sacrifice 21 days later; P. carinii nuclei were reduced by 91.2 and >99.99%, respectively. This effect was reversed by concomitant administration of AdoMet with nicotine. Treatment with AdoMet alone increased infection intensity. We conclude that AdoMet is a critical and limiting nutrient for Pneumocystis thus can serve as a therapeutic target for PCP. Regarding the mechanism, nicotine treatment caused no change in rat lung activity of AdoMet synthesizing methionine ATP transferase activity nor was there any evidence of increased AdoMet utilization for methylation reactions. Except of a doubling of putrescine, nicotine treatment also did not change lung polyamine content. However, key polyamine anabolic and catabolic enzymes were upregulated, and there were corresponding changes in polyamine metabolic intermediates. We conclude that chronic nicotine treatment increases lung polyamine catabolic/anabolic cycling and/or excretion leading to increased AdoMet-consuming polyamine biosynthesis and depletion of lung AdoMet.
- Published
- 2005
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.