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19 results on '"Milcova, Alena"'

3. The Genotoxicity of Organic Extracts from Particulate Emissions Produced by Neat Gasoline (E0) and a Gasoline–Ethanol Blend (E15) in BEAS-2B Cells

Author

Libalova, Helena, primary, Zavodna, Tana, additional, Elzeinova, Fatima, additional, Barosova, Hana, additional, Cervena, Tereza, additional, Milcova, Alena, additional, Vankova, Jolana, additional, Paradeisi, Foteini, additional, Vojtisek-Lom, Michal, additional, Sikorova, Jitka, additional, Topinka, Jan, additional, and Rossner, Pavel, additional

Published
2023
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4. The Genotoxicity of Organic Extracts from Particulate Emissions Produced by Neat Gasoline (E0) and a Gasoline–Ethanol Blend (E15) in BEAS-2B Cells.

Author

Libalova, Helena, Zavodna, Tana, Elzeinova, Fatima, Barosova, Hana, Cervena, Tereza, Milcova, Alena, Vankova, Jolana, Paradeisi, Foteini, Vojtisek-Lom, Michal, Sikorova, Jitka, Topinka, Jan, and Rossner, Pavel

Subjects
GENETIC toxicology, ETHANOL, DNA repair, ISOBUTANOL, GASOLINE, POLYCYCLIC aromatic hydrocarbons, ARYL hydrocarbon receptors, DNA damage
Abstract

Emissions from modern gasoline engines represent an environmental and health risk. In this study, we aimed to compare the toxicity of organic compound mixtures extracted from particulate matter (PM extracts) produced by neat gasoline (E0) and a blend containing 15% ethanol (E15), which is offered as an alternative to non-renewable fossil fuels. Human lung BEAS-2B cells were exposed to PM extracts, and biomarkers of genotoxicity, such as DNA damage evaluated by comet assay, micronuclei formation, levels of phosphorylated histone H2AX, the expression of genes relevant to the DNA damage response, and exposure to polycyclic aromatic hydrocarbons (PAHs), were determined. Results showed that both PM extracts significantly increased the level of oxidized DNA lesions. The E0 extract exhibited a more pronounced effect, possibly due to the higher content of nitrated PAHs. Other endpoints were not substantially affected by any of the PM extracts. Gene expression analysis revealed mild but coordinated induction of genes related to DNA damage response, and a strong induction of PAH-inducible genes, indicating activation of the aryl hydrocarbon receptor (AhR). Our data suggest that the addition of ethanol into the gasoline diminished the oxidative DNA damage, but no effect on other genotoxicity biomarkers was observed. Activated AhR may play an important role in the toxicity of gasoline PM emissions. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Oxidative Stress and Antioxidant Response in Populations of the Czech Republic Exposed to Various Levels of Environmental Pollutants

Author

Ambroz, Antonin, primary, Rossner, Pavel, additional, Rossnerova, Andrea, additional, Honkova, Katerina, additional, Milcova, Alena, additional, Pastorkova, Anna, additional, Klema, Jiri, additional, Pulkrabova, Jana, additional, Parizek, Ondrej, additional, Vondraskova, Veronika, additional, Zelenka, Jaroslav, additional, Vrzáčková, Nikola, additional, Schmuczerova, Jana, additional, Topinka, Jan, additional, and Sram, Radim J., additional

Published
2022
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8. Genome-Wide DNA Methylation in Policemen Working in Cities Differing by Major Sources of Air Pollution

Author

Honkova, Katerina, primary, Rossnerova, Andrea, additional, Chvojkova, Irena, additional, Milcova, Alena, additional, Margaryan, Hasmik, additional, Pastorkova, Anna, additional, Ambroz, Antonin, additional, Rossner, Pavel, additional, Jirik, Vitezslav, additional, Rubes, Jiri, additional, Sram, Radim J., additional, and Topinka, Jan, additional

Published
2022
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11. Gene Expression and Epigenetic Changes in Mice Following Inhalation of Copper(II) Oxide Nanoparticles

Author

Rossner, Pavel, primary, Vrbova, Kristyna, additional, Rossnerova, Andrea, additional, Zavodna, Tana, additional, Milcova, Alena, additional, Klema, Jiri, additional, Vecera, Zbynek, additional, Mikuska, Pavel, additional, Coufalik, Pavel, additional, Capka, Lukas, additional, Krumal, Kamil, additional, Docekal, Bohumil, additional, Holan, Vladimir, additional, Machala, Miroslav, additional, and Topinka, Jan, additional

Published
2020
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12. The processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter

Author

Rossner, Pavel, primary, Libalova, Helena, additional, Cervena, Tereza, additional, Vrbova, Kristyna, additional, Elzeinova, Fatima, additional, Milcova, Alena, additional, Rossnerova, Andrea, additional, Novakova, Zuzana, additional, Ciganek, Miroslav, additional, Pokorna, Michaela, additional, Ambroz, Antonin, additional, and Topinka, Jan, additional

Published
2019
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13. processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter.

Author

Rossner, Pavel, Libalova, Helena, Cervena, Tereza, Vrbova, Kristyna, Elzeinova, Fatima, Milcova, Alena, Rossnerova, Andrea, Novakova, Zuzana, Ciganek, Miroslav, Pokorna, Michaela, Ambroz, Antonin, and Topinka, Jan

Subjects
GLYCOGEN synthase kinase-3, POLYCYCLIC aromatic hydrocarbons, PARTICULATE matter, PEROXIDATION, LIPIDS, FIBROBLASTS
Abstract

Polycyclic aromatic hydrocarbons (PAHs) may cause lipid peroxidation via reactive oxygen species generation. 15-F2t-isoprostane (IsoP), an oxidative stress marker, is formed from arachidonic acid (AA) by a free-radical induced oxidation. AA may also be converted to prostaglandins (PG) by prostaglandin-endoperoxide synthase (PTGS) induced by NF-κB. We treated human embryonic lung fibroblasts (HEL12469) with benzo[a]pyrene (B[a]P), 3-nitrobenzanthrone (3-NBA) and extractable organic matter (EOM) from ambient air particulate matter <2.5 µm for 4 and 24 h. B[a]P and 3-NBA induced expression of PAH metabolising, but not antioxidant enzymes. The concentrations of IsoP decreased, whereas the levels of AA tended to increase. Although the activity of NF-κB was not detected, the tested compounds affected the expression of prostaglandin-endoperoxide synthase 2 (PTGS2). The levels of prostaglandin E2 (PGE2) decreased following exposure to B[a]P, whereas 3-NBA exposure tended to increase PGE2 concentration. A distinct response was observed after EOM exposure: expression of PAH-metabolising enzymes was induced, IsoP levels increased after 24-h treatment but AA concentration was not affected. The activity of NF-κB increased after both exposure periods, and a significant induction of PTGS2 expression was found following 4-h treatment. Similarly to PAHs, the EOM exposure was associated with a decrease of PGE2 levels. In summary, exposure to PAHs with low pro-oxidant potential results in a decrease of IsoP levels implying 'antioxidant' properties. For such compounds, IsoP may not be a suitable marker of lipid peroxidation. [ABSTRACT FROM AUTHOR]

Published
2019
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