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9. Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode

Author

1000070709633, Kato, Masaru, Masuda, Yuya, Yoshida, Narumi, 1000000548993, Tosha, Takehiko, 1000070183051, Shiro, Yoshitsugu, 1000040292776, Yagi, Ichizo, 1000070709633, Kato, Masaru, Masuda, Yuya, Yoshida, Narumi, 1000000548993, Tosha, Takehiko, 1000070183051, Shiro, Yoshitsugu, 1000040292776, and Yagi, Ichizo

Abstract

In protein film electrochemistry for redox-active membrane proteins, the reconstitution of protein-tethered bilayer lipid membranes (ptBLMs) can maximize their functionality and stability at electrolyte/electrode interfaces. To understand the impact of protein–lipid interactions on the ptBLM formation, we tracked the reconstitution process of a lipid bilayer in the presence or the absence of a transmembrane enzyme of cytochrome c-dependent nitric oxide reductase (cNOR) on a mixed self-assembled monolayer (SAM)/Au electrode. Time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy and electrochemical measurements revealed that the protein–lipid interaction affected the formation kinetics for the phospholipid bilayer and the electrocatalytic stability of the cNOR-modified electrode but not the fluidity of the phospholipid bilayer.

Published
2021

10. Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode

Author

Kato, Masaru, Masuda, Yuya, Yoshida, Narumi, Tosha, Takehiko, Shiro, Yoshitsugu, Yagi, Ichizo, Kato, Masaru, Masuda, Yuya, Yoshida, Narumi, Tosha, Takehiko, Shiro, Yoshitsugu, and Yagi, Ichizo

Abstract

In protein film electrochemistry for redox-active membrane proteins, the reconstitution of protein-tethered bilayer lipid membranes (ptBLMs) can maximize their functionality and stability at electrolyte/electrode interfaces. To understand the impact of protein–lipid interactions on the ptBLM formation, we tracked the reconstitution process of a lipid bilayer in the presence or the absence of a transmembrane enzyme of cytochrome c-dependent nitric oxide reductase (cNOR) on a mixed self-assembled monolayer (SAM)/Au electrode. Time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy and electrochemical measurements revealed that the protein–lipid interaction affected the formation kinetics for the phospholipid bilayer and the electrocatalytic stability of the cNOR-modified electrode but not the fluidity of the phospholipid bilayer.

Published
2021

13. Evaluation of noninvasive positive pressure ventilation after extubation from moderate positive end-expiratory pressure level in patients undergoing cardiovascular surgery: a prospective observational study

Author

Suzuki, Takeshi, primary, Kurazumi, Takuya, additional, Toyonaga, Shinya, additional, Masuda, Yuya, additional, Morita, Yoshihisa, additional, Masuda, Junichi, additional, Kosugi, Shizuko, additional, Katori, Nobuyuki, additional, and Morisaki, Hiroshi, additional

Published
2014
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14. Coexistence of Cold Agglutinin and Cryoglobulin in a Patient With Severe Anemia Following COVID-19 Infection.

Author

Kaneko M, Masuda Y, Ishikawa K, and Shikata H

Abstract

We report a case of coexisting cold agglutinin and cryoglobulin in a patient with severe anemia following COVID-19 infection, in whom direct antiglobulin testing revealed C3d positivity and immunoglobulin G negativity. There was no evident hemolytic anemia, thrombosis, or clinically significant IgM monoclonal gammopathy. The anemia improved with folic acid supplementation alone accompanied by a decrease of the cold agglutination titer, and the direct antiglobulin test became negative. Despite continuous positivity for cryoglobulinemia, the patient exhibited no clinical symptoms such as thrombosis. Immunosuppressive therapy for cold-related hematologic disorders after COVID-19 infection should be considered carefully., Competing Interests: Human subjects: Consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Kaneko et al.)

Published
2024
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