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1. Resistance mechanisms to TP53-MDM2 inhibition identified by in vivo piggyBac transposon mutagenesis screen in an Arf−/− mouse model

Author

Chapeau, Emilie A., Gembarska, Agnieszka, Durand, Eric Y., Mandon, Emeline, Estadieu, Claire, Romanet, Vincent, Wiesmann, Marion, Tiedt, Ralph, Lehar, Joseph, deWeck, Antoine, Rad, Roland, Barys, Louise, Jeay, Sebastien, Ferretti, Stephane, Kauffmann, Audrey, Sutter, Esther, Grevot, Armelle, Moulin, Pierre, Murakami, Masato, Sellers, William R., Hofmann, Francesco, and Jensen, Michael Rugaard

Published
2017

2. A conditional inducible JAK2V617F transgenic mouse model reveals myeloproliferative disease that is reversible upon switching off transgene expression

Author

Chapeau, Emilie A., primary, Mandon, Emeline, additional, Gill, Jason, additional, Romanet, Vincent, additional, Ebel, Nicolas, additional, Powajbo, Violetta, additional, Andraos-Rey, Rita, additional, Qian, Zhiyan, additional, Kininis, Miltos, additional, Zumstein-Mecker, Sabine, additional, Ito, Moriko, additional, Hynes, Nancy E., additional, Tiedt, Ralph, additional, Hofmann, Francesco, additional, Eshkind, Leonid, additional, Bockamp, Ernesto, additional, Kinzel, Bernd, additional, Mueller, Matthias, additional, Murakami, Masato, additional, Baffert, Fabienne, additional, and Radimerski, Thomas, additional

Published
2019
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3. Resistance mechanisms to TP53-MDM2 inhibition identified by in vivo piggyBac transposon mutagenesis screen in an Arf −/− mouse model

Author

Chapeau, Emilie A., primary, Gembarska, Agnieszka, additional, Durand, Eric Y., additional, Mandon, Emeline, additional, Estadieu, Claire, additional, Romanet, Vincent, additional, Wiesmann, Marion, additional, Tiedt, Ralph, additional, Lehar, Joseph, additional, de Weck, Antoine, additional, Rad, Roland, additional, Barys, Louise, additional, Jeay, Sebastien, additional, Ferretti, Stephane, additional, Kauffmann, Audrey, additional, Sutter, Esther, additional, Grevot, Armelle, additional, Moulin, Pierre, additional, Murakami, Masato, additional, Sellers, William R., additional, Hofmann, Francesco, additional, and Jensen, Michael Rugaard, additional

Published
2017
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4. A conditional inducible JAK2V617F transgenic mouse model reveals myeloproliferative disease that is reversible upon switching off transgene expression.

Author

Chapeau, Emilie A., Mandon, Emeline, Gill, Jason, Romanet, Vincent, Ebel, Nicolas, Powajbo, Violetta, Andraos-Rey, Rita, Qian, Zhiyan, Kininis, Miltos, Zumstein-Mecker, Sabine, Ito, Moriko, Hynes, Nancy E., Tiedt, Ralph, Hofmann, Francesco, Eshkind, Leonid, Bockamp, Ernesto, Kinzel, Bernd, Mueller, Matthias, Murakami, Masato, and Baffert, Fabienne

Subjects
*TRANSGENIC mice, *HEMATOPOIETIC stem cells, *POLYCYTHEMIA vera, *BONE marrow cells, *TRANSGENE expression, *DISEASES
Abstract

Aberrant activation of the JAK/STAT pathway is thought to be the critical event in the pathogenesis of the chronic myeloproliferative neoplasms, polycythemia vera, essential thrombocythemia and primary myelofibrosis. The most frequent genetic alteration in these pathologies is the activating JAK2V617F mutation, and expression of the mutant gene in mouse models was shown to cause a phenotype resembling the human diseases. Given the body of genetic evidence, it has come as a sobering finding that JAK inhibitor therapy only modestly suppresses the JAK2V617F allele burden, despite showing clear benefits in terms of reducing splenomegaly and constitutional symptoms in patients. To gain a better understanding if JAK2V617F is required for maintenance of myeloproliferative disease once it has evolved, we generated a conditional inducible transgenic JAK2V617F mouse model using the SCL-tTA-2S tet-off system. Our model corroborates that expression of JAK2V617F in hematopoietic stem and progenitor cells recapitulates key hallmarks of human myeloproliferative neoplasms, and exhibits gender differences in disease manifestation. The disease was found to be transplantable, and importantly, reversible when transgenic JAK2V617F expression was switched off. Our results indicate that mutant JAK2V617F-specific inhibitors should result in profound disease modification by disabling the myeloproliferative clone bearing mutant JAK2. [ABSTRACT FROM AUTHOR]

Published
2019
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5. CHZ868, a Type II JAK2 Inhibitor, Reverses Type I JAK Inhibitor Persistence and Demonstrates Efficacy in Myeloproliferative Neoplasms

Author

Meyer, Sara C., primary, Keller, Matthew D., additional, Chiu, Sophia, additional, Koppikar, Priya, additional, Guryanova, Olga A., additional, Rapaport, Franck, additional, Xu, Ke, additional, Manova, Katia, additional, Pankov, Dmitry, additional, O’Reilly, Richard J., additional, Kleppe, Maria, additional, McKenney, Anna Sophia, additional, Shih, Alan H., additional, Shank, Kaitlyn, additional, Ahn, Jihae, additional, Papalexi, Eftymia, additional, Spitzer, Barbara, additional, Socci, Nick, additional, Viale, Agnes, additional, Mandon, Emeline, additional, Ebel, Nicolas, additional, Andraos, Rita, additional, Rubert, Joëlle, additional, Dammassa, Ernesta, additional, Romanet, Vincent, additional, Dölemeyer, Arno, additional, Zender, Michael, additional, Heinlein, Melanie, additional, Rampal, Raajit, additional, Weinberg, Rona Singer, additional, Hoffman, Ronald, additional, Sellers, William R., additional, Hofmann, Francesco, additional, Murakami, Masato, additional, Baffert, Fabienne, additional, Gaul, Christoph, additional, Radimerski, Thomas, additional, and Levine, Ross L., additional

Published
2015
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6. Activity of the Type II JAK2 Inhibitor CHZ868 in B Cell Acute Lymphoblastic Leukemia

Author

Wu, Shuo-Chieh, primary, Li, Loretta S., additional, Kopp, Nadja, additional, Montero, Joan, additional, Chapuy, Bjoern, additional, Yoda, Akinori, additional, Christie, Amanda L., additional, Liu, Huiyun, additional, Christodoulou, Alexandra, additional, van Bodegom, Diederik, additional, van der Zwet, Jordy, additional, Layer, Jacob V., additional, Tivey, Trevor, additional, Lane, Andrew A., additional, Ryan, Jeremy A., additional, Ng, Samuel Y., additional, DeAngelo, Daniel J., additional, Stone, Richard M., additional, Steensma, David, additional, Wadleigh, Martha, additional, Harris, Marian, additional, Mandon, Emeline, additional, Ebel, Nicolas, additional, Andraos, Rita, additional, Romanet, Vincent, additional, Dölemeyer, Arno, additional, Sterker, Dario, additional, Zender, Michael, additional, Rodig, Scott J., additional, Murakami, Masato, additional, Hofmann, Francesco, additional, Kuo, Frank, additional, Eck, Michael J., additional, Silverman, Lewis B., additional, Sallan, Stephen E., additional, Letai, Anthony, additional, Baffert, Fabienne, additional, Vangrevelinghe, Eric, additional, Radimerski, Thomas, additional, Gaul, Christoph, additional, and Weinstock, David M., additional

Published
2015
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7. Resistance mechanisms to TP53-MDM2 inhibition identified by in vivo piggyBac transposon mutagenesis screen in an Arf-/- mouse model.

Author

Chapeau, Emilie A., Gembarska, Agnieszka, Durand, Eric Y., Mandon, Emeline, Estadieu, Claire, Romanet, Vincent, Wiesmann, Marion, Tiedt, Ralph, Lehar, Joseph, de Weck, Antoine, Rad, Roland, Barys, Louise, Jeay, Sebastien, Ferretti, Stephane, Kauffmann, Audrey, Sutter, Esther, Grevot, Armelle, Moulin, Pierre, Murakami, Masato, and Sellers, William R.

Subjects
MUTAGENESIS, LABORATORY mice, TRANSPOSONS, COHORT analysis, PHARMACOLOGY
Abstract

Inhibitors of double minute 2 protein (MDM2)-tumor protein 53 (TP53) interaction are predicted to be effective in tumors in which the TP53 gene is wild type, by preventing TP53 protein degradation. One such setting is represented by the frequent CDKN2A deletion in human cancer that, through inactivation of p14ARF, activates MDM2 protein, which in turn degrades TP53 tumor suppressor. Here we used piggyBac (PB) transposon insertional mutagenesis to anticipate resistance mechanisms occurring during treatment with the MDM2-TP53 inhibitor HDM201. Constitutive PB mutagenesis in Arf-/- mice provided a collection of spontaneous tumors with characterized insertional genetic landscapes. Tumors were allografted in large cohorts of mice to assess the pharmacologic effects of HDM201. Sixteen out of 21 allograft models were sensitive to HDM201 but ultimately relapsed under treatment. A comparison of tumors with acquired resistance to HDM201 and untreated tumors identified 87 genes that were differentially and significantly targeted by the PB transposon. Resistant tumors displayed a complex clonality pattern suggesting the emergence of several resistant subclones. Among the most frequent alterations conferring resistance, we observed somatic and insertional loss-of-function mutations in transformation-related protein 53 (Trp53) in 54% of tumors and transposonmediated gain-of-function alterations in B-cell lymphoma-extra large (Bcl-xL), Mdm4, and two TP53 family members, resulting in expression of the TP53 dominant negative truncations △NTrp63 and △NTrp73. Enhanced BCL-xL and MDM4 protein expression was confirmed in resistant tumors, as well as in HDM201-resistant patient-derived tumor xenografts. Interestingly, concomitant inhibition of MDM2 and BCL-xL demonstrated significant synergy in p53 wild-type cell lines in vitro. Collectively, our findings identify several potential mechanisms by which TP53 wild-type tumors may escape MDM2-targeted therapy. [ABSTRACT FROM AUTHOR]

Published
2017
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