322 results on '"Mammina, Caterina"'
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2. Burden and viral aetiology of influenza-like illness and acute respiratory infection in intensive care units
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Tramuto, Fabio, Maida, Carmelo Massimo, Napoli, Giuseppe, Mammina, Caterina, Casuccio, Alessandra, Cala', Cinzia, Amodio, Emanuele, and Vitale, Francesco
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- 2016
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3. Molecular Characterization of Quinolone Resistance Determinants in Non-Typhoidal Salmonella Strains Isolated in Tehran, Iran
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Helali-Pargali, Razie, primary, Ranjbar, Reza, additional, Memariani, Mojtaba, additional, Najafi, Ali, additional, and Mammina, Caterina, additional
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- 2022
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4. Immunoglobulin M seropositivity for Toscana virus in a random population sample in Sicily
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Amodio, Emanuele, Cusi, Maria Grazia, Valenti, Rosalia Maria, Valentini, Melissa, Mammina, Caterina, Gori-Savellini, Gianni, Vitale, Francesco, Romano, Nino, Goedert, James J., and Calamusa, Giuseppe
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- 2012
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5. MRSA ST22-IVa (EMRSA-15 clone) in Palermo, Italy
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Bonura, Celestino, Plano, Maria Rosa Anna, Di Carlo, Paola, Calà, Cinzia, Cipolla, Domenico, Corsello, Giovanni, and Mammina, Caterina
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- 2010
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6. A combined molecular typing approach does not discriminate Legionella pneumophila serogroup 1 strains of a predominant sequence-based type in Palermo, Italy
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Bonura, Celestino, Mammina, Caterina, Vella, Antonietta, Belfiore, Santina, Chiarini, Alfredo, and Giammanco, Anna
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- 2009
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7. Additional file 2 of Food safety in hospital: knowledge, attitudes and practices of nursing staff of two hospitals in Sicily, Italy
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Buccheri, Cecilia, Casuccio, Alessandra, Giammanco, Santo, Giammanco, Marco, La Guardia, Maurizio, and Mammina, Caterina
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Data_FILES - Abstract
Authors’ original file for figure 1
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- 2021
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8. Additional file 1 of Food safety in hospital: knowledge, attitudes and practices of nursing staff of two hospitals in Sicily, Italy
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Buccheri, Cecilia, Casuccio, Alessandra, Giammanco, Santo, Giammanco, Marco, La Guardia, Maurizio, and Mammina, Caterina
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Additional file 1: Questionnaire about food safety knowledge, attitudes and practices of nursing staff of two hospitals in Sicily, Italy. It included five sections: a) demographic characteristics, employment status and hospital/ward where the nurse worked; b) knowledge about food hygiene; c) attitudes towards prevention of foodborne diseases; d) measures to be used in prevention of foodborne diseases; e) sources of information about food hygiene. (DOC 33 KB)
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- 2021
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9. Evaluation of a modified single-enzyme amplified fragment length polymorphism (SE-AFLP) technique for subtyping Salmonella enterica serotype Enteritidis
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Giammanco, Giovanni M., Mammina, Caterina, Romani, Cristina, Luzzi, Ida, Dionisi, Anna Maria, and Nastasi, Antonino
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- 2007
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10. Salmonella enterica serotype Typhimurium DT 104 antibiotic resistance genomic Island I in serotype Paratyphi B
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Meunier, Daniele, Boyd, David, Mulvey, Michael R., Baucheron, Sylvie, Mammina, Caterina, Nastasi, Antonino, Chaslus-Dancla, Elisabeth, and Cloeckaert, Axel
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Salmonella -- Research ,Drug resistance in microorganisms -- Research - Abstract
We have identified Salmonella genomic island I (SGI1) in an isolate of Salmonella enterica serotype Paratyphi B. This antibiotic-resistance gene cluster, which confers multidrug resistance, has been previously identified in [...]
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- 2002
11. Global distribution of Sigella sonnei clones
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Filliol-Toutain, Ingrid, Chiou, Chien-Shun, Mammina, Caterina, Gerner-Smidt, Peter, Thong, Kwai-Lin, Phung, Dac Cam, Pichel, Mariana, Ranjbar, Reza, Sow, Amy Gassama, Cooper, Kara, Ribot, Efrain, Binsztein, Norma, and Liang, Shiu-Yun
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Cloning -- Analysis -- Research ,Genetically modified organisms -- Research ,Shigella -- Physiological aspects -- Research ,Health - Abstract
Shigella sonnei is the most commonly isolated species O among the 4 Shigella species in industrialized countries (1,2). Transmission of S. sonnei across geographic boundaries is frequently linked to international [...]
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- 2011
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12. Food safety in hospital: knowledge, attitudes and practices of nursing staff of two hospitals in Sicily, Italy
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Buccheri, Cecilia, Casuccio, Alessandra, Giammanco, Santo, Giammanco, Marco, La Guardia, Maurizio, and Mammina, Caterina
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- 2007
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13. Probable disseminated Mycobacterium abscessus subspecies bolletii infection in a patient with idiopathic CD4+ T lymphocytopenia: a case report
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Colomba Claudia, Rubino Raffaella, Di Carlo Paola, Mammina Caterina, Bonura Celestino, Siracusa Lucia, Titone Lucina, and Saporito Laura
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CD4+ T lymphocytopenia ,Clarithromycin ,Disseminated infection ,Mycobacterium abscessus subsp. bolletii rapidly growing mycobacteria ,Medicine - Abstract
Abstract Introduction Rapidly growing mycobacteria are opportunistic pathogens in patients with underlying risk factors. Mycobacterium abscessus subsp. bolletii is a newly recognized member of rapidly growing mycobacteria, isolated from respiratory tract and cutaneous infections. Case presentation We describe a case of chronic disseminated infection caused by M. abscessus subsp. bolletii in a 38-year-old Sri Lankan man with idiopathic CD4+ T lymphocytopenia. Idiopathic CD4+ T lymphocytopenia is a rare cause of immunodysfunction that, similar to human immunodeficiency virus infection, causes a depletion of CD4+ T lymphocytes. M. abscessus subsp. bolletii infection was diagnosed by culture isolation from two sputum samples. Conclusions To the best of our knowledge this is the first report of M. abscessus subsp. bolletii disseminated infection in a patient affected by idiopathic CD4+ T lymphocytopenia. In contrast to previous reports, the isolate of M. abscessus subsp. bolletii presented intermediate resistance to clarithromycin and was susceptible to cefoxitin and imipenem.
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- 2012
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14. Epidemiology and clonality of carbapenem-resistant Acinetobacter baumannii from an intensive care unit in Palermo, Italy
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Mammina Caterina, Palma Daniela, Bonura Celestino, Aleo Aurora, Fasciana Teresa, Sodano Concetta, Saporito Maria, Verde Maria, Calà Cinzia, Cracchiolo Andrea, and Tetamo Romano
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Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Background Multidrug-resistant Acinetobacter baumannii, initially considered as having a poor clinical relevance, is frequently isolated from infection cases in intensive care units. We describe the epidemiology of carbapenem resistant A. baumannii (CRAB) in a general ICU in Palermo, Italy, from October 2010 to March 2011. Findings 58 of 61 isolates exhibited MICs for meropenem or imipenem ≥16 mg/L. Forty-nine carried blaOXA-23 and two blaOXA-58 genes. Five subtype clusters were detected by rep-PCR. Clusters D and E included 10 isolates that tested negative for the carbapenem resistance genes. MLST attributed all isolates, but two, with sequence type (ST)2, whereas the two remaining isolates with ST78. The respiratory tract was the most common site of infection (26 out of 36 cases. 72.2%). A high infection related mortality rate was observed (18 out of 35 patients, 51.4%). Nineteen patients tested positive for other multidrug resistant organisms in addition to CRAB. In eight cases isolates belonging to distinct subtype clusters and/or with distinct carbapenemase profiles were identified. Conclusions Carbapenem resistance was prominently driven by the dissemination of CRAB isolates belonging to ST2, carrying the carbapenemase gene blaOXA-23. The colonization/infection of some patients by multiple strains is suggestive of an endemic circulation of CRAB.
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- 2012
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15. Polyclonal non multiresistant methicillin resistant Staphylococcus aureus isolates from clinical cases of infection occurring in Palermo, Italy, during a one-year surveillance period
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Mammina Caterina, Calà Cinzia, Bonura Celestino, Di Carlo Paola, Aleo Aurora, Fasciana Teresa, and Giammanco Anna
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Therapeutics. Pharmacology ,RM1-950 ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Abstract
Abstract Background The evolving epidemiology of methicillin resistant Staphylococcus aureus (MRSA) is characterized by the emergence of infections caused by non multiresistant MRSA carrying staphylococcal chromosomal cassette (SCC)mec IV or V in the healthcare settings. A molecular epidemiological analysis of non multiresistant MRSA isolates from four acute general hospitals was performed in Palermo, Italy, during a one year period. Methods For the purpose of the study, MRSA isolates were defined as non multiresistant when they were susceptible to at least three classes of non β-lactam antibiotics. Seventy-five isolates were submitted to antimicrobial susceptibility testing, multilocus sequence typing (MLST) and polymerase chain reaction (PCR) for SCCmec, accessory gene regulator (agr) groups, arginine catabolic mobile element (ACME) and Panton Valentine leukocidin (PVL) toxin genes. For epidemiological typing, Multiple-Locus Variable-Number Tandem Repeat Fingerprinting (MLVF) was performed on all isolates and pulsed field gel electrophoresis (PFGE) on ST8 isolates. Results Non multiresistant MRSA isolates were isolated from all hospitals. Resistances to ciprofloxacin, macrolides and tetracycline were the most prevalent. MLST attributed 46 isolates with ST22, 13 with ST8, eight with ST1, three with ST50 and three with ST398. SCCmec type IV was found in all isolates. PVL was detected in one ST22 isolate. All isolates tested negative for the ACME element. MLVF identified 31 different patterns, some subtype clusters ranging in size between two and 22 isolates. The closely related PFGE patterns of the ST8 isolates differed from USA300. Conclusions A polyclonal circulation of non multiresistant MRSA along with blurring of boundaries between healthcare associated (HA)-MRSA and community associated (CA)-MRSA appear to be occurring in our epidemiological setting. A better understanding of spread of MRSA with the support of molecular typing can provide invaluable information in the epidemiological, microbiological and clinical fields.
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- 2012
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16. Epidemic spread of ST1-MRSA-IVa in a neonatal intensive care unit, Italy
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Giuffrè Mario, Cipolla Domenico, Bonura Celestino, Geraci Daniela, Aleo Aurora, Di Noto Stefania, Nociforo Federica, Corsello Giovanni, and Mammina Caterina
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CA-MRSA ,NICU ,Epidemiology ,Infection control ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has recently emerged as an important pathogen in neonatal intensive care units (NICUs). The purposes of this study were to characterize methicillin-resistant isolates from an outbreak in a NICU, to examine the genetic traits and clonality of CA-MRSA, and to review the characteristics and outcomes of the neonatal cases and investigate the routes of entry and transmission of the MRSA outbreak strain in the NICU under study. Methods The study NICU practiced an active surveillance program for multidrug-resistant organisms, including weekly cultures for detection of MRSA from nasal swabs among all the admitted neonates. All first isolates from surveillance cultures and all clinical isolates were submitted for susceptibility testing and genotyping. Data from each infant’s medical records were prospectively included in a database, and the clinical features and outcomes of the colonized/infected infants were assessed. Results A total of 14 infants were colonized or infected by a strain of ST1-MRSA-IVa between April and August 2011. The CA-MRSA strain appeared to have been introduced to the NICU by an infected infant transferred from another hospital. The outbreak was successfully contained by multifaceted infection control interventions. Conclusions The results of this study confirm that NICU is a healthcare setting with a critical permeability to CA-MRSA. Active surveillance including molecular typing can help to detect and monitor the spread of antimicrobial drug-resistant organisms, and thus trigger timely control interventions.
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- 2012
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17. Protein supplementation in strength and conditioning adepts: knowledge, dietary behavior and practice in Palermo, Italy
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Bianco Antonino, Mammina Caterina, Paoli Antonio, Bellafiore Marianna, Battaglia Giuseppe, Caramazza Giovanni, Palma Antonio, and Jemni Monèm
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Nutrition. Foods and food supply ,TX341-641 ,Sports medicine ,RC1200-1245 - Abstract
Abstract Background It is known that supplement use is a widespread and accepted practice by athletes and people who attend commercial gyms. Little is known about protein supplement amongst people undertaking strength training in commercial gyms in Italy when compared to the US. Objective The purpose of this study was to examine the use of protein supplementation, alone or in association with other supplements, and dietary behavior amongst regular fitness center attendees in Palermo, Italy. Design Resistance training information have been collected from 800 regular fitness center attendees for the initial analysis. A specific questionnaire was generated for the experimentation. Data were collected using a face-to-face interview method. Supplement users were then compared to the non users and analyzed using a one-way ANOVA, Kruskall-Wallis, chi-square test or exact test of Fisher when appropriate. Results 30.1% of the respondents use dietary supplements during their training as a believe it is the "way to gain muscles and strength". Whey protein shakes (50.0%) mixed with creatine and amino-acids (48.3%) were the most frequent choices amongst the users. A majority of the subjects (34.0%) appeared to rely on their gym instructors' advice for their intake; a lower proportion (13.0%) consulted physicians, while none of them consulted nutritionists. A high consumption of milk has been noticed in both users (67,7%) and non-users (52,8%); supplement non-users consumed significantly more snacks and bakery products than users per week (P < 0.001), while users consumed significantly more protein-rich foods (P < 0.01) with a particular preference for meat (48.0%). Conclusions A considerable number of regular strength training adepts consume protein supplements mixed with other products (mainly creatine and amino-acids). Limited numbers consult "dietary specialists" and rely mainly on their instructors. We emphasize on the importance of the dissemination of scientifically based information about supplementation in this environment and the promotion of updated educational programs for the instructors.
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- 2011
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18. Lymphocyte apoptosis in children with central nervous system tuberculosis: a case control study
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Carlo Paola Di, Casuccio Alessandra, Romano Amelia, Spicola Daria, Titone Lucina, Caccamo Nadia, Dieli Francesco, Mammina Caterina, Pace Elisabetta, Gjomarkaj Mark, Melis Mario, and Tolomeo Manlio
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Pediatrics ,RJ1-570 - Abstract
Abstract Background Studies of the apoptosis mechanisms involved in the pathogenesis of tuberculosis have suggested that Mycobacterium tuberculosis can actively interfere with the apoptosis of infected cells. In vivo studies have been performed in adult populations but have not focused on this process in children. In the present study, we analyzed spontaneous T lymphocyte (PBT) apoptosis in the peripheral blood of children with central nervous system tuberculosis (CNS TB), before and after chemotherapy, and compared the results with healthy controls. Methods A case-control study was conducted from January 2002 to June 2009. It included 18 children with CNS TB and 17 healthy controls. Spontaneous apoptosis of PBTs, including CD4+, CD8+ and CD8+/CD28+ T cells, was evaluated after 24 and 72 h of culture in complete medium, using the Annexin V detection test. Analysis was conducted before and after chemotherapy, and expression of the apoptotic markers CD95 (Fas) and Fas ligand (FasL) was evaluated. Results Higher percentages of apoptotic T cells and CD4 lymphocytes were isolated from children with acute phase CNS TB than from children in the control group (p < 0.05). This difference significantly decreased after 60 days of specific treatment. In children with CNS TB, high levels of Fas ligand expression were detected in lymphocyte populations, associated with a high percentage of Fas positive cells, before and after treatment. In contrast to the CD4+ apoptosis profile, we did not find any significant difference in total CD8+ cell apoptosis between children with acute phase disease and the control group. However, the percentage of apoptotic CD8+/CD28+ T cells was significantly higher in the children with acute phase disease than in the healthy controls. Conclusions Our findings indicate that CNS TB in pediatric patients increases the sensitivity of CD4 and CD8+/CD28+ T cells to apoptosis, suggesting a hypoergic status of this infection. This could play a key role in the immunopathogenesis of this complicated form of TB. Interestingly, specific chemotherapy is able to normalize both apoptosis sensitivity and T-cell activation.
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- 2011
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19. Two cases of monomicrobial intraabdominal abscesses due to KPC - 3 Klebsiella pneumoniae ST258 clone
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Madonia Simona, Latteri Adelfio M, Gulotta Gaspare, Giammanco Anna, D'Arpa Francesco, Cusimano Alessia, Pantuso Gianni, Di Carlo Paola, Salamone Giuseppe, and Mammina Caterina
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monomicrobial abscess ,Klebsiellae pneumoniae ,carbapenemases ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Background Knowledge of the etiology of pyogenic liver and pancreatic abscesses is an important factor in determining the success of combined surgical and antibiotic treatment. Literature shows geographical variations in the prevalence and distribution of causative organisms, and the spread of Klebsiella pneumoniae carbapenemase-producing bacteria is an emerging cause of abdominal infections. Case presentation We herein describe two cases of intra-abdominal abscesses due to monomicrobial infection by Klebsiella pneumoniae Sequence Type 258 producing K. pneumoniae carbapenemase 3 (KPC-Kp). In case 1, a 50-year-old HIV-negative Italian woman with chronic pancreatitis showed infection of a pancreatic pseudocystic lesion caused by KPC-Kp. In case 2, a 64-year-old HIV- negative Italian woman with pancreatic neoplasm and liver metastases developed a liver abscess due to KPC after surgery. Both women were admitted to our hospital but to different surgical units. The clonal relationship between the two isolates was investigated by pulsed-field gel electrophoresis (PFGE). In case 2, the patient was already colonized at admission and inter-hospital transmission of the pathogen was presumed. A long-term combination regimen of colistin with tigecycline and percutaneous drainage resulted in full recovery and clearance of the multidrug-resistant (MDR) pathogen. Conclusions Timely microbiological diagnosis, the combined use of new and old antibiotics and radiological intervention appeared to be valuable in managing these serious conditions. The emergence and dissemination of MDR organisms is posing an increasing challenge for physicians to develop new therapeutic strategies and control and prevention frameworks.
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- 2011
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20. A survey on knowledge and self-reported formula handling practices of parents and child care workers in Palermo, Italy
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Mammina Caterina, Guida Ivana, Valenti Rosalia, and Calamusa Giuseppe
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Pediatrics ,RJ1-570 - Abstract
Abstract Background Powdered infant formula (PIF) is not a sterile product, but this information appears to be poorly diffused among child caregivers. Parents and child care workers may behave in an unsafe manner when handling PIF. Methods This study involved parents and child care workers in the 24 municipal child care centres of Palermo. Knowledge and self-reported practices about PIF handling were investigated by a structured questionnaire. A Likert scale was used to measure the strength of the respondent's feelings. Association of knowledge and self-reported practices with demographic variables was also evaluated. Results 42.4% of parents and 71.0% of child care workers filled in the questionnaire. Significant differences were found between parents and child care workers for age and education. 73.2% of parents and 84.4% of child care workers were confident in sterility of PIF. Generally, adherence to safe procedures when reconstituting and handling PIF was more frequently reported by child care workers who, according to the existing legislation, are regularly subjected to a periodic training on food safety principles and practices. Age and education significantly influenced the answers to the questionnaire of both parents and child care workers. Conclusion The results of the study reveal that parents and child care workers are generally unaware that powdered formulas may contain viable microorganisms. However, child care workers consistently chose safer options than parents when answering the questions about adherence to hygienic practices. At present it seems unfeasible to produce sterile PIF, but the risk of growth of hazardous organisms in formula at the time of administration should be minimized by promoting safer behaviours among caregivers to infants in both institutional settings and home.
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- 2009
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21. Double copies of Tn4401a:blaKPC-3 on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy
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Fortini Daniela, Villa Laura, Pires João, Bonura Celestino, Mammina Caterina, Endimiani Andrea, and Carattoli Alessandra
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A carbapenem resistant sequence type (ST) 512 KPC 3 producing Klebsiella pneumoniae strain showing a novel variant plasmid content was isolated in Palermo Italy in 2014. ST512 is a worldwide successful clone associated with the spread of blaKPC genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain the blaKPC 3 was exceptionally located on an IncX3 plasmid whose complete sequence was determined. Two copies of the Tn44011a:blaKPC 3 transposon due to a intramolecular transposition events were detected in the plasmid.
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- 2015
22. Reinterpreting a community outbreak of Salmonella enterica serotype Enteritidis in the light of molecular typing
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Nastasi Antonino, Buonomini Maria, Nicoletti PierLuigi, Romani Cristina, and Mammina Caterina
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Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background In November 2005, a large outbreak due to Salmonella enterica serotype Enteritidis (S. Enteritidis) was observed within children who had eaten their meals at 53 school cafeterias in Florence and the surrounding area. A total of 154 isolates of S. Enteritidis were recovered from human cases between November 2005 and January 2006. All strains were assigned phage type 8 (PT8) and a common XbaI pulsotype. This paper reports the findings of a molecular epidemiological investigation performed on 124 strains of S. Enteritidis isolated in the years 2005 and 2006 in Florence and the surrounding area, including the epidemic isolates. Methods One hundred twenty-four human isolates of S. Enteritidis identified in the period January 2005 – December 2006 were submitted to molecular typing by single enzyme – amplified fragment length polymorphism (SE-AFLP). Results Molecular subtyping by SE-AFLP yielded five different profiles. In the pre-epidemic phase, type A included 78.4% of isolates, whereas only three (8.1%) belonged to type C. All isolates, but one, of the epidemic phase were indistinguishable and attributed to type C. In the post-epidemic period, a polymorphic pattern of SE-AFLP types was again recognized but type C accounted for 73.3% of the isolates during the first six months of 2006, whereas during the remaining six months type A regained the first place, including 52.0% of the isolates. Conclusion The epidemic event was attributed to the emergence and clonal expansion of a strain of S. Enteritidis PT8-SE-AFLP type C. Circulation of the epidemic clone was much more extensive than the surveillance and traditional laboratory data demonstrated.
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- 2007
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23. Genetic relatedness among isolates of Shigella sonnei carrying class 2 integrons in Tehran, Iran, 2002–2003
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Sadeghifard Nourkhoda, Dionisi Anna, Giammanco Giovanni M, Aleo Aurora, Ranjbar Reza, and Mammina Caterina
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Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Shigella spp. are major cause of diarrhoeal disease in both developing and developed countries. Shigella sonnei is the serogroup of Shigella most frequently responsible for sporadic and epidemic enteritis in developed countries. In recent years the emergence and spread of S. sonnei biotype g carrying class 2 integron have been frequently reported in many countries. Recently, S. sonnei has been reported as the prevalent serogroup of Shigella in Iran. The present study was carried out to investigate phenotypic and genetic characteristics of Shigella sonnei isolates identified in the years 2002 and 2003 in Tehran, Iran. Methods Biotyping, drug susceptibility testing, pulsed field gel electrophoresis (PFGE) and analysis of class 2 integrons have been carried out on 60 S. sonnei isolates, including 57 sporadic isolates from paediatric cases of shigellosis occurring in 2002 and 2003, two sporadic isolates recovered in 1984 and the ATCC 9290 strain. Results Biotype g and resistance to streptomycin, sulfamethoxazole-trimethoprim and tetracycline were exhibited by 54 of the 57 recent isolates. Of the 54 biotype g isolates, 28 exhibited a class 2 integron of 2161 bp, and 24 a class 2 integron of 1371 bp, respectively. Class 2 integrons were not detected in four isolates only, including the two endemic isolates recovered in 1984 and two strains from recent sporadic cases. PFGE divided the strains into eight pulsotypes labeled A to H, three major pulsotypes – A to C – including the large majority of the recent sporadic S. sonnei isolates. Pulsotypes A and C were the most prevalent groups, accounting for 41.6% and 35.0%, respectively, of the isolates under study. Conclusion The results suggest that biotype g, class 2 integron carrying S. sonnei are prevalent in our geographic area. S. sonnei isolated in the years 2002 and 2003 could be attributed to a few predominant clusters including, respectively, strains with pulsotypes B and C carrying a 2161 bp class 2 integron, and those having pulsotype A and a 1371 bp class 2 integron. A few epidemic clones are responsible for the apparently endemic occurrence of shigellosis in Tehran, Iran.
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- 2007
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24. Food safety in hospital: knowledge, attitudes and practices of nursing staff of two hospitals in Sicily, Italy
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La Guardia Maurizio, Giammanco Marco, Giammanco Santo, Casuccio Alessandra, Buccheri Cecilia, and Mammina Caterina
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Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Food hygiene in hospital poses peculiar problems, particularly given the presence of patients who could be more vulnerable than healthy subjects to microbiological and nutritional risks. Moreover, in nosocomial outbreaks of infectious intestinal disease, the mortality risk has been proved to be significantly higher than the community outbreaks and highest for foodborne outbreaks. On the other hand, the common involvement in the role of food handlers of nurses or domestic staff, not specifically trained about food hygiene and HACCP, may represent a further cause of concern. The purpose of this study was to evaluate knowledge, attitudes, and practices concerning food safety of the nursing staff of two hospitals in Palermo, Italy. Association with some demographic and work-related determinants was also investigated. Methods The survey was conducted, by using a semi-structured questionnaire, in March-November 2005 in an acute general hospital and a paediatric hospital, where nursing staff is routinely involved in food service functions. Results Overall, 401 nurses (279, 37.1%, of the General Hospital and 122, 53.5%, of the Paediatric Hospital, respectively) answered. Among the respondents there was a generalized lack of knowledge about etiologic agents and food vehicles associated to foodborne diseases and proper temperatures of storage of hot and cold ready to eat foods. A general positive attitude towards temperature control and using clothing and gloves, when handling food, was shared by the respondents nurses, but questions about cross-contamination, refreezing and handling unwrapped food with cuts or abrasions on hands were frequently answered incorrectly. The practice section performed better, though sharing of utensils for raw and uncooked foods and thawing of frozen foods at room temperatures proved to be widely frequent among the respondents. Age, gender, educational level and length of service were inconsistently associated with the answer pattern. More than 80% of the respondent nurses did not attend any educational course on food hygiene. Those who attended at least one training course fared significantly better about some knowledge issues, but no difference was detected in both the attitude and practice sections. Conclusion Results strongly emphasize the need for a safer management of catering in the hospitals, where non professional food handlers, like nursing or domestic staff, are involved in food service functions.
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- 2007
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25. Global distribution of Mycobacterium tuberculosis spoligotypes. (Dispatches)
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Filliol, Ingrid, Driscoll, Jeffrey R., van Soolingen, Dick, Kreiswirth, Barry N., Kremer, Kristin, Valetudie, Georges, Anh, Dang Duc, Barlow, Rachael, Banerjee, Dilip, Bifani, Pablo J., Brudey, Karine, Cataldi, Angel, Cooksey, Robert C., Cousins, Debby V., Dale, Jeremy W., Dellagostin, Odir A., Drobniewski, Francis, Engelmann, Guido, Ferdinand, Severine, GascoyneBinzi, Deborah, Gordon, Max, Gutierrez, M. Cristina, Haas, Walter H., Heersma, Herre, Kallenius, Gunilla, Kassa-Kelembho, Eric, Koivula, Tuija, Ly, Ho Minh, Makristathis, Athanasios, Mammina, Caterina, Martin, Gerald, Mostrom, Peter, Mokrousov, Igor, Narbonne, Valerie, Narvskaya, Olga, Nastasi, Antonino, Niobe-Eyangoh, Sara Ngo, Pape, Jean W, Rasolofo-Razanamparany, Voahangy, Ridell, Malin, Rossetti, M. Lucia, Stauffer, Fritz, Suffys, Philip N., Takiff, Howard, Texier-Maugein, Jeanne, Vincent, Veronique, de Waard, Jacobus H., Sola, Christophe, and Rastogi, Nalin
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CD-ROM catalog ,CD-ROM database ,Database ,Tuberculosis -- Research ,Tuberculosis -- Physiological aspects ,Mycobacteria -- Physiological aspects ,Mycobacteria -- Research ,Mycobacterium ,Databases -- Usage - Abstract
We present a short summary of recent observations on the global distribution of the major clades of the Mycobacterium tuberculosis complex, the causative agent of tuberculosis. This global distribution was [...]
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- 2002
26. Shigella sonnei biotype g carrying class 2 integrons in southern Italy: a retrospective typing study by pulsed field gel electrophoresis
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Romani Cristina, Aleo Aurora, Mammina Caterina, and Nastasi Antonino
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Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Emergence and global dissemination of multiresistant strains of enteric pathogens is a very concerning problem from both epidemiological and Public Health points of view. Shigella sonnei is the serogroup of Shigella most frequently responsible for sporadic and epidemic enteritis in developed countries. The dissemination is associated most often to human to human transmission, but foodborne episodes have also been described. In recent years the circulation of multiresistant strains of S. sonnei biotype g carrying a class 2 integron has been reported in many countries worldwide. In southern Italy a strain with similar properties has been responsible for a large community outbreak occurred in 2003 in Palermo, Sicily. The objective of this study was to date the emergence of the biotype g strain carrying the class 2 integron in southern Italy and to evaluate the genetic heterogeneity of biotype g S. sonnei isolated throughout an extended interval of time. Methods A total of 31 clinical isolates of S. sonnei biotype g identified in southern Italy during the years 1971–2000 were studied. The strains were identified at the serogroup level, characterized by biochemical tests and submitted to antimicrobial susceptibility testing. Molecular typing was performed by pulsed field gel electrophoresis (PFGE) after digestion of DNA by XbaI. Carriage of class 2 integrons was investigated by polymerase chain reaction (PCR) with specific primers and confirmed by restriction endonuclease analysis of amplicons. Results The 15 isolates of S. sonnei biotype g identified in the decade 1971–1980 showed highly heterogeneous drug resistance profiles and pulsotypes. None of the isolates was simultaneous resistant to streptomycin and trimethoprim and none was class 2 integron positive. On the contrary, this resistance phenotype and class 2 integron carriage were very common among the 16 strains of biotype g identified in the following two decades. Moreover, all the more recent isolates, but one, showed closely related pulsotypes. Conclusion Although our findings refer to a limited geographic area, they provide a snapshot of integron acquisition by an enteric pathogen responsible for several outbreaks in the years 2001–2003 in Italy. Molecular typing, indeed, suggests that the emergence of biotype g class 2 integron carrying S. sonnei in southern Italy should be backdated to at least the late 1980s. In the following decades, the circulation of biotype g appears to be sustained by multiresistant highly related strains. Similar trend are described in several countries, but the questions about mechanism of emergence and worldwide spread of this pathogen remain open.
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- 2006
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27. Evidence for Human Adaptation and Foodborne Transmission of Livestock-Associated Methicillin-Resistant Staphylococcus aureus
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Larsen, Jesper, Stegger, Marc, Andersen, Paal S, Petersen, Andreas, Larsen, Anders R, Westh, Henrik, Agersø, Yvonne, Fetsch, Alexandra, Kraushaar, Britta, Käsbohrer, Annemarie, Feβler, Andrea T, Schwarz, Stefan, Cuny, Christiane, Witte, Wolfgang, Butaye, Patrick, Denis, Olivier, Haenni, Marisa, Madec, Jean-Yves, Jouy, Eric, Laurent, Frederic, Battisti, Antonio, Franco, Alessia, Alba, Patricia, Mammina, Caterina, Pantosti, Annalisa, Monaco, Monica, Wagenaar, Jaap A, de Boer, Enne, van Duijkeren, Engeline, Heck, Max, Domínguez, Lucas, Torres, Carmen, Zarazaga, Myriam, Price, Lance B, Skov, Robert L, LS Klinisch Onderzoek Wagenaar, and dI&I I&I-4
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livestock ,poultry ,MRSA ,host adaptation ,foodborne transmission - Abstract
We investigated the evolution and epidemiology of a novel livestock-associated methicillin-resistant Staphylococcus aureus strain, which colonizes and infects urban-dwelling Danes even without a Danish animal reservoir. Genetic evidence suggests both poultry and human adaptation, with poultry meat implicated as a probable source.
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- 2016
28. Erratum to: Enhanced surveillance of invasive listeriosis in the Lombardy region, Italy, in the years 2006-2010 reveals major clones and an increase in serotype 1/2a [BMC Infect Dis., 13, (2013), 152.]
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Mammina, Caterina, Parisi, Antonio, Guaita, Anna, Aleo, Aurora, Bonura, Celestino, NASTASI, Antonino, Pontello, Mirella, Mammina, Caterina, Parisi, Antonio, Guaita, Anna, Aleo, Aurora, Bonura, Celestino, Nastasi, Antonino, and Pontello, Mirella
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Invasive listeriosi ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,surveillance - Published
- 2015
29. A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
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GERACI, Daniela Maria, GIUFFRE, Mario, BONURA, Celestino, GRAZIANO, Giorgio, ALEO, Aurora, VECCHIO, Davide, MAMMINA, Caterina, SAPORITO, Laura, Insinga, V., Rinaudo, G., Ciofalo, A., Vitaliti, M., Lunetta, F., Sulliotti, G., Pinna, G., Pomo, R., Rizzo, A., Sepporta, V., Geraci, D., Giuffrè, M., Bonura, C., Graziano, G., Saporito, L., Insinga, V., Rinaudo, G., Aleo, A., Vecchio, D., Mammina, C., Ciofalo, A., Vitaliti, M., Lunetta, F., Sulliotti, G., Pinna, G., Pomo, R., Rizzo, A., and Sepporta, V.
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0301 basic medicine ,Microbiology (medical) ,medicine.medical_specialty ,Neonatal intensive care unit ,health care facilities, manpower, and services ,030106 microbiology ,lcsh:QR1-502 ,MRSA ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,Internal medicine ,Epidemiology ,Medicine ,Infection control ,network approach ,Original Research ,NICu ,Active surveillance ,Molecular typing ,Network approach ,business.industry ,MRSA colonization ,active surveillance ,molecular typing ,Sequence types ,Variable number tandem repeat ,030104 developmental biology ,Multilocus sequence typing ,University teaching ,business - Abstract
Objectives. We performed a one-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods. Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014 – January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at four week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF) and multilocus sequence typing (MLST). Results. In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157 and 95 from NICUs A, C, D and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A-F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions. Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities.
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- 2016
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30. The Increasing Challenge of Multidrug-Resistant Gram-Negative Bacilli
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Giuffrè, Mario, Geraci, Daniela M., Bonura, Celestino, Saporito, Laura, Graziano, Giorgio, Insinga, Vincenzo, Aleo, Aurora, Vecchio, Davide, and Mammina, Caterina
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Cross Infection ,Time Factors ,Incidence ,Infant, Newborn ,Observational Study ,Length of Stay ,Anti-Bacterial Agents ,Italy ,Risk Factors ,Drug Resistance, Multiple, Bacterial ,Intensive Care Units, Neonatal ,Population Surveillance ,Gram-Negative Bacteria ,Prevalence ,Humans ,Prospective Studies ,Gram-Negative Bacterial Infections ,Research Article ,Follow-Up Studies - Abstract
Colonization and infection by multidrug-resistant gram-negative bacilli (MDR GNB) in neonatal intensive care units (NICUs) are increasingly reported. We conducted a 5-year prospective cohort surveillance study in a tertiary NICU of the hospital “Paolo Giaccone,” Palermo, Italy. Our objectives were to describe incidence and trends of MDR GNB colonization and the characteristics of the most prevalent organisms and to identify the risk factors for colonization. Demographic, clinical, and microbiological data were prospectively collected. Active surveillance cultures (ASCs) were obtained weekly. Clusters of colonization by extended spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae were analyzed by conventional and molecular epidemiological tools. During the study period, 1152 infants were enrolled in the study. Prevalences of colonization by MDR GNB, ESBL-producing GNB and multiple species/genera averaged, respectively, 28.8%, 11.7%, and 3.7%. Prevalence and incidence density of colonization by MDR GNB and ESBL-producing GNB showed an upward trend through the surveillance period. Rates of ESBL-producing E coli and K pneumoniae colonization showed wide fluctuations peaking over the last 2 years. The only independent variables associated with colonization by MDR GNB and ESBL-producing organisms and multiple colonization were, respectively, the days of NICU stay (odds ratio [OR] 1.041), the days of exposure to ampicillin–sulbactam (OR 1.040), and the days of formula feeding (OR 1.031). Most clusters of E coli and K pneumoniae colonization were associated with different lineages. Ten out of 12 clusters had an outborn infant as their index case. Our study confirms that MDR GNB are an increasing challenge to NICUs. The universal once-a-week approach allowed us to understand the epidemiology of MDR GNB, to timely detect new clones and institute contact precautions, and to assess risk factors. Collection of these data can be an important tool to optimize antimicrobials use and control the emergence and dissemination of resistances in NICU.
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- 2016
31. Artemisia arborescens essential oil composition, enantiomeric distribution and antimicrobial activity from different wild populations from the Mediterranean Area
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El-Amin Said , Mohammed, Militello , Marcello, Saia , Sergio, Settanni , Luca, Aleo , Aurora, Mammina , Caterina, Bombarda , Isabelle, Vanloot , Pierre, Roussel , Christian, Dupuy , Nathalie, Said , Mohammed El-Amin, Department of Agricultural and Forest Sciences, University of Palermo, Council for Agricultural Research and Economics ( CREA ), Sciences for Health Promotion, Laboratoire d'Instrumentation et Sciences Analytiques ( LISA ), Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Institut des Sciences Moléculaires de Marseille ( ISM2 ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Centrale de Marseille ( ECM ) -Aix Marseille Université ( AMU ), Systèmes Chimiques Complexes - Formulation - Qualité - Environnement ( SCCFQE ), Centre National de la Recherche Scientifique ( CNRS ) -Université Paul Cézanne - Aix-Marseille 3-Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Université de Provence - Aix-Marseille 1-Université de la Méditerranée - Aix-Marseille 2, Laboratoire d'Instrumentation et Sciences Analytiques (LISA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Palermo - University of Palermo, Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria (CREA), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC), Said, M.E.–A, Militello, M, Saia, S, Settanni, L, Aleo, A, Mammina, C, Bombarda, I, Vanloot, P, Roussel, C, Dupuy, N, Council for Agricultural Research and Economics (CREA), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Systèmes Chimiques Complexes - Formulation - Qualité - Environnement (SCCFQE), Centre National de la Recherche Scientifique (CNRS)-Université Paul Cézanne - Aix-Marseille 3-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Avignon Université (AU)-Université de Provence - Aix-Marseille 1-Université de la Méditerranée - Aix-Marseille 2, Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics (CREA), and Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,Listeria ,Bioengineering ,Microbial Sensitivity Tests ,Settore MED/42 - Igiene Generale E Applicata ,01 natural sciences ,Biochemistry ,[ CHIM ] Chemical Sciences ,law.invention ,Terpene ,Camphor ,chemistry.chemical_compound ,law ,Botany ,Oils, Volatile ,[CHIM]Chemical Sciences ,Food science ,Molecular Biology ,Essential oil ,Volatile composition ,biology ,Chemotype ,Mediterranean Region ,Chemistry ,Chamazulene ,Biological activity ,Stereoisomerism ,General Chemistry ,General Medicine ,biology.organism_classification ,Artemisia arborescens ,Enantiomeric distribution ,Listeria monocytogenes ,Anti-Bacterial Agents ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Settore MED/18 - Chirurgia Generale ,Artemisia ,Italy ,Algeria ,Gram-negative bacteria ,Molecular Medicine ,Gas chromatography ,Enantiomeric distribution, Biological activity, Gram-negative bacteria, Volatile composition, Listeria monocytogenes ,010606 plant biology & botany ,Settore AGR/16 - Microbiologia Agraria - Abstract
International audience; Aerial parts of Artemisiaarborescens were collected from different sites of the Mediterranean area (southwestern Algeria and southern Italy) and the chemical composition of their essential oil (EO) extracted by hydrodistillation was studied by both gas chromatography (GC) equipped with an enantioselective capillary column and GC/mass spectrometry (GC/MS). The EOs obtained were tested against several Listeriamonocytogenes strains. Using GC and GC/MS, 41 compounds were identified, accounting for 96.0-98.8% of the total EO. All EOs showed a similar terpene profile, which was rich in chamazulene, -thujone, and camphor. However, the concentration of such compounds varied among the EOs. A.arborescens EO inhibited up to 83.3% of the L.monocytogenes strains, but the inhibitory spectrum varied among the EOs, with those from Algeria showing a higher inhibition degree than the Italian EOs. Such effect likely depended on the ketone (-thujone+camphor) content of the EO. The differences in the EO composition support the hypothesis that A.arborescens has at least two different chemotypes: a -thujone and a chamazulene type. The EO inhibitory spectrum indicates the A.arborescens EO as a valuable option in the control of the food-borne pathogens.Artemisiaarborescens Essential Oil Composition, Enantiomeric Distribution, and Antimicrobial Activity from Different Wild Populations from the Mediterranean Area
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- 2016
32. Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone
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Villa, Laura, primary, Feudi, Claudia, additional, Fortini, Daniela, additional, Brisse, Sylvain, additional, Passet, Virginie, additional, Bonura, Celestino, additional, Endimiani, Andrea, additional, Mammina, Caterina, additional, Ocampo, Ana Maria, additional, Jimenez, Judy Natalia, additional, Doumith, Michel, additional, Woodford, Neil, additional, Hopkins, Katie, additional, and Carattoli, Alessandra, additional
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- 2017
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33. Influenza like Illness among Medical Residents Anticipates Influenza Diffusion in General Population: Data from a National Survey among Italian Medical Residents
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Restivo, Vincenzo, primary, Costantino, Claudio, additional, Mammina, Caterina, additional, and Vitale, Francesco, additional
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- 2016
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34. Bloodstream infections in intensive care unit patients: distribution and antibiotic resistance of bacteria
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Cortegiani, Andrea, Russotto,Vincenzo, Graziano,Giorgio, Saporito,Laura, Raineri,Santi Maurizio, Mammina,Caterina, and Giarratano,Antonino
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Infection and Drug Resistance - Abstract
Vincenzo Russotto,1 Andrea Cortegiani,1 Giorgio Graziano,2 Laura Saporito,2 Santi Maurizio Raineri,1 Caterina Mammina,2 Antonino Giarratano1 1Department of Biopathology and Medical Biotechnologies (DIBIMED), Section of Anaesthesia, Analgesia, Intensive Care and Emergency, Paolo Giaccone University Hospital, University of Palermo, Palermo, Italy; 2Department of Sciences for Health Promotion and Mother-Child Care, University of Palermo, Palermo, Italy Abstract: Bloodstream infections (BSIs) are among the leading infections in critically ill patients. The case-fatality rate associated with BSIs in patients admitted to intensive care units (ICUs) reaches 35%–50%. The emergence and diffusion of bacteria with resistance to antibiotics is a global health problem. Multidrug-resistant bacteria were detected in 50.7% of patients with BSIs in a recently published international observational study, with methicillin resistance detected in 48% of Staphylococcus aureus strains, carbapenem resistance detected in 69% of Acinetobacter spp., in 38% of Klebsiella pneumoniae, and in 37% of Pseudomonas spp. Prior hospitalization and antibiotic exposure have been identified as risk factors for infections caused by resistant bacteria in different studies. Patients with BSIs caused by resistant strains showed an increased risk of mortality, which may be explained by a higher incidence of inappropriate empirical therapy in different studies. The molecular genetic characterization of resistant bacteria allows the understanding of the most common mechanisms underlying their resistance and the adoption of surveillance measures. Knowledge of epidemiology, risk factors, mechanisms of resistance, and outcomes of BSIs caused by resistant bacteria may have a major influence on global management of ICU patients. The aim of this review is to provide the clinician an update on BSIs caused by resistant bacteria in ICU patients. Keywords: bloodstream infections, multidrug resistant, antibiotic, intensive care unit, MDR, ICU
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- 2015
35. Respiratory Tularemia: Francisella Tularensis and Microarray Probe Designing
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Ranjbar, Reza, primary, Behzadi, Payam, additional, and Mammina, Caterina, additional
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- 2016
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36. Staphylococcal cassette chromosome mec typing and mecA sequencing in methicillin-resistant staphylococci from Algeria: a highly diversified element with new mutations in mecA
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Djoudi, Ferhat, primary, Bonura, Celestino, additional, Touati, Abdelaziz, additional, Aléo, Aurora, additional, Benallaoua, Said, additional, and Mammina, Caterina, additional
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- 2016
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37. Surveillance of methicillin-resistant Staphylococcus aureus, multidrug-resistant Gram-negatives and fungi colonization in the Neonatal Intensive Care Units in the city of Palermo
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GRAZIANO, Giorgio, SAPORITO, Laura, GERACI, Daniela Maria, INSINGA, Vincenzo, RINAUDO, Grazia, MAIDA, Carmelo Massimo, GIUFFRE, Mario, MAMMINA, Caterina, Graziano, G, Saporito, L, Geraci, DM, Insinga, V, Rinaudo, G, Maida, CM, Giuffrè, M, and Mammina, C
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Settore MED/38 - Pediatria Generale E Specialistica ,multidrug resistant, surveillance, NICU ,Settore MED/42 - Igiene Generale E Applicata - Abstract
Introduction Multidrug-resistant organisms are a serious public health problem, particularly in critical hospital wards such as the Neonatal Intensive Care Units (NICUs). Hospitalized neonates are at high risk for healthcare-associated infections leading to higher morbidity and mortality rates, because of their poor immune defences, frequent exposure to antibiotics, invasive procedures and contacts with healthcare-workers. Our study aimed to evaluate the prevalence of colonization by methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Gram-negatives (MDRGNs) and fungi in the five NICUs of the city of Palermo. Methods From February 2014 to January 2015, an active surveillance culture (ACS) program was set up in the five NICUs of Palermo (ARNAS Civico-NICU1, Ingrassia Hospital-NICU2, Villa Sofia-Cervello Hospital-NICU3, Buccheri La Ferla Hospital-NICU4 and AOUP "Paolo Giaccone"-NICU5). From each patient nasal and rectal swabs were monthly collected to look for MRSA, MDRGNs and fungi. Swabs were pre-incubated at 37°C overnight in BHI enrichment broth, and cultured on selective media. Suspected S. aureus colonies were confirmed by cefoxitin susceptibility testing. MDRGNs were selected by antibiotic disk screening and tested to detect extended-spectrum beta-lactamase” (ESBL) and carbapenemase-producing isolates. For fungal detection swabs were directly inoculated on chloramphenicol-Sabouraud dextrose agar plates and incubated at 30°C for 72 hrs. Yeast-like colonies were identified by subculturing on CHROMagar Candida and eventually confirmed by the API20CAUX test. Prevalence rates of colonization of the different NICUs were compared by the chi-square test. Results The average daily number of hospitalized patients was 16 in NICU1, 9 in NICU2, 21 in NICU3, 15 in NICU4, 11 in NICU5. Prevalence of MRSA colonization ranged between 3.6% in NICU3 and 28.8% in NICU4, showing statistically significant differences between the NICUs. Prevalence of colonization by MDRGNs ranged between 53.9% for NICU1 and 14% for NICU4 with statistically significant differences between the NICUs. The most frequently isolated MDRGN organisms were Klebsiella pneumoniae, Escherichia coli and Enterobacter spp., with a prevalence between 1.9% and 57.2% for ESBL-producing and between 0% and 3.5% for carbapenemase-producing isolates. Fungal colonization was never detected in NICU2, whilst the highest prevalence values were 7.2% for C. albicans in NICU4 and 5.9% for non-albicans Candida spp. in NICU5. Statistically significant differences were found. Discussion Spread of multidrug-resistant organisms proved to affect all the NICUs under investigation, with a higher prevalence of MRSA in NICUs 4 and 2 and a higher prevalence of MDRGNs in NICUs 1 and 5. Fungal colonization prevalence was different between the NICUs with NICU4 being more affected by C.albicans and NICU5 by non-albicans Candida spp. These differences could be attributable to different structural and organizational characteristics and the recurrent epidemic spread of some organisms by cross-transmission. The widespread dissemination of MRSA and ESBL producing isolates and the emergence of carbapenem-resistant Enterobacteriaceae require a continuous effort in implementing preventive measures, control interventions and staff training.
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- 2015
38. Adapted Physical Activity for the Promotion of Health and the Prevention of Multifactorial Chronic Diseases: the Erice Charter
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Spica, Vincenzo Romano, Macini, Pierluigi, Galeone, Daniela, Liguori, Giorgio, Signorelli, Carlo, Marensi, Lorenzo, Vagali, Pierluigi, Guberti, Emilia, Goletti, Mauro, Mammina, Caterina, Tabacchi, Garden, Izzotti, Alberto, Cinquetti, Sandro, Pellizzari, Barbara, Beltrami, Patrizia, Alonzo, Elena, Gradilone, Antonio, Leoni, Erica, Buono, Pasqualina, Brandi, Giorgio, Rosa, Enrico Di, Parisi, Attilio, Capolongo, Stefano, Fantuzzi, Guglielmina, Aggazzotti, Gabriella, Grillo, Claudio, Borella, Paola, Manzoli, Lamberto, Fabiani, Leila, Faggiano, Fabrizio, Mete, Rosario, Privitera, GAETANO PIERPAOLO, Coniglio, Maria Anna, Frangella, Claudia, Rossi, Daniela, Lagorio, Silvia, Pasquarella, Maria Luisa, Isabella, Albino, Tripi, Ferdinando, Franceschetti, Romano, Commare, Antonella La, Gallè, Francesca, Brandimarte, Maria Alessandra, Savino, Gustavo, Onofrio, Valeria Di, Tortorella, Franco, Gabriele, Michele, Monreale, Vincenzo, Abrignani, Maurizio, Ferreri, Giovanni, Cacciapuoti, Antonio, Valeriani, Federica, Raffo, Marisa, Matarese, Massimo, Ripani, Maurizio, Altana, Valentina, Antonioni, Stefania, Anzalone, Concetta, Astorino, Gerardo, Azzollini, Annunziata, Belfiore, Patrizia, Valentini, Fabio Besozzi, Bragazzi, Nicola Luigi, Calaciura, Antonella, Casano, Diego, Ciulla, Antonio, Cono, Osvaldo Ernandez, Contrisciani, Roberta, Cosenza, Bruno, D’Aloisio, Francesco, Antonino, De Francesco, Marchis, Antonella De, Francesco, Di Nardo, Fabri, Serena, Famiglietti, Elena, Gandolfi, Angela, Genovese, Cristina, Genovesi, Antonio, Gianfredi, Vincenza, Gigante, Sebastiano, Gigliotti, Alfredina, Iacona, Claudia, Innocenzi, Ludovico, Laurita, Vincenzo, Maratea, Fabio, Meletti, Luca, Memmini, Silvia, Messana, Maria Anna, Morelli, Cristina, Nappi, Maria Rosaria, Nucci, Daniele, Orlandi, Pierluigi, Palamara, Maria Angela Rita, Patti, Anna, Persi, Ylenia, Polisano, Benedetta, Roccaro, Davide, Ricchiuti, Roberta, Sanclemente, Leonardo, Scanavini, Sara, Sirtori, Mario Donato, Soncini, Francesco, Stelitano, Gilda Immacolata, Valenzano, Anna Antonia, Veicsteinas, Arsenio, Fara, Gaetano Maria, Giammanco, Giuseppe, Spica, V. R., Macini, P., Galeone, D., Liguori, G., Signorelli, C., Marensi, L., Vagali, P., Guberti, E., Goletti, M., Mammina, C., Tabacchi, G., Izzotti, A., Cinquetti, S., Pellizzari, B., Beltrami, P., Alonzo, E., Gradilone, A., Leoni, E., Buono, P., Brandi, G., Di Rosa, E., Parisi, A., Capolongo, S., Fantuzzi, G., Aggazzotti, G., Grillo, C., Borella, P., Manzoli, L., Fabiani, L., Faggiano, F., Mete, R., Privitera, G., Coniglio, M. A., Frangella, C., Rossi, D., Lagorio, S., Pasquarella, M. L., Isabella, A., Tripi, F., Franceschetti, R., Commare, A. L., Galle, F., Brandimarte, M. A., Savino, G., Di Onofrio, V., Tortorella, F., Gabriele, M., Monreale, V., Abrignani, M., Ferreri, G., Cacciapuoti, A., Valeriani, F., Raffo, M., Matarese, M., Ripani, M., Altana, V., Antonioni, S., Anzalone, C., Astorino, G., Azzollini, A., Belfiore, P., Valentini, F. B., Bragazzi, N. L., Calaciura, A., Casano, D., Ciulla, A., Cono, O. E., Contrisciani, R., Cosenza, B., D'Aloisio, F., Antonino, D. F., Marchis, A. D., Francesco, D. N., Fabri, S., Famiglietti, E., Gandolfi, A., Genovese, C., Genovesi, A., Gianfredi, V., Gigante, S., Gigliotti, A., Iacona, C., Innocenzi, L., Laurita, V., Maratea, F., Meletti, L., Memmini, S., Messana, M. A., Morelli, C., Nappi, M. R., Nucci, D., Orlandi, P., Palamara, M. A. R., Patti, A., Persi, Y., Polisano, B., Roccaro, D., Ricchiuti, R., Sanclemente, L., Scanavini, S., Sirtori, M. D., Soncini, F., Stelitano, G. I., Valenzano, A. A., Veicsteinas, A., Fara, G. M., and Giammanco, G.
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Biomedical Research ,Adapted physical activity ,Health promotion ,Prevention ,Chronic Disease ,Health Promotion ,Humans ,Italy ,Motor Activity ,Physicians ,Primary Prevention ,Specialization ,Public Health ,Prevention parole chiave ,Physical Activity, Health Promotion, Prevention, Chronic Diseases, Erice Charter ,Physical Activity ,Erice Charter ,Attività fisica adattata ,Prevenzione ,Promozione della salute ,Chronic Diseases ,health promotion ,prevention - Abstract
The Erice Charter was unanimously approved at the conclusion of the 47th Residential Course "Adapted Physical Activity in Sport, Wellness and Fitness: New Challenges for Prevention and Health Promotion", held on 20-24 April 2015 in Erice, Italy, at the "Ettore Majorana" Foundation and Centre for Scientific Culture, and promoted by the International School of Epidemiology and Preventive Medicine "G. D'Alessandro" and the Study Group on Movement Sciences for Health of the Italian Society of Hygiene, Preventive Medicine and Public Health. After an intense discussion the participants identified the main points associated with the relevance of physical activity for Public Health, claiming the pivotal role of the Department of Prevention in coordinating and managing preventive actions. The participants underlined the importance of the physicians specialized in Hygiene, Preventive Medicine and Public Health. The contribution of other operators such as physicians specialized in Sport Medicine was stressed. Further, the holders of the new degree in Human Movement and Sport Sciences were considered fundamental contributors for the performance of physical activity and their presence was seen as a promising opportunity for the Departments of Prevention. Primary prevention based on recreational physical activities should become easily accessible for the population, avoiding obstacles such as certification steps or complex bureaucracy. The Sport Doctor is recognized as the principal referent for preliminary physical evaluation and clinical monitoring in secondary and tertiary prevention actions based on adapted physical activities. Developing research in the field is essential as well as implementing higher education on physical activity management in Schools of Public Health.
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- 2015
39. Use of cepheid xpert carba-r® for rapid detection of carbapenemase-producing bacteria in abdominal septic patients admitted to intensive care unit
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Cortegiani, A, Russotto, V, Graziano, G, Geraci, D, Saporito, L, Cocorullo, G, Raineri, S, Mammina, C, Giarratano, A, Cortegiani, Andrea, Russotto, Vincenzo, Graziano, Giorgio, Geraci, Daniela, Saporito, Laura, Cocorullo, Gianfranco, Raineri, Santi Maurizio, Mammina, Caterina, Giarratano, Antonino, Cortegiani, A, Russotto, V, Graziano, G, Geraci, D, Saporito, L, Cocorullo, G, Raineri, S, Mammina, C, Giarratano, A, Cortegiani, Andrea, Russotto, Vincenzo, Graziano, Giorgio, Geraci, Daniela, Saporito, Laura, Cocorullo, Gianfranco, Raineri, Santi Maurizio, Mammina, Caterina, and Giarratano, Antonino
- Abstract
Early institution of effective antibiotic therapy and source control are pivotal to improve survival of abdominal septic patients. Xpert® Carba-R is a real time polymerase chain reaction assay for rapid detection and differentiation of five genes (blaKPC , blaVIM , blaOXA-48 , blaIMP-1 , blaNDM ) responsible for carbapenem resistance. We performed an observational study investigating the clinical usefulness and applicability of Xpert® Carba-R to detect carbapenem resistance in abdominal septic patients admitted to intensive care unit. We compared the results of Xpert® Carba-R with standard microbiological culture. We collected a set of two rectal/stomia swabs and two swabs from abdominal drainage fluid for each patient. We included 20 patients for a total of 45 comparisons between the two methods. In our clinical setting, the overall performance of Xpert® Carba-R for detection of carbapenem resistance in the presence of genes detectable and non-detectable by the method was: sensitivity 50% (95% CI 24.6-75.3); specificity 93.1% (95% CI 77.2-99.1); positive predictive value (PPV) 80% (95% CI 44.4-97.5); negative predictive value (NPV) 77.1% (95% CI 56.9-89.6). The inter-rater agreement was 0.47 (SE 0.14; 95% CI 0.20-0.74). When considering the only 5 mechanisms of resistance detected by both methods, the overall diagnostic performance was: sensitivity 100% (95% CI 69.1-100), specificity 94.2 (95% CI 80.8-99.3), PPV 83.3 (95% CI 59.6-97.9) and NPV 100% (95% CI 89.4-100). The inter-rater agreement was 0.88 (SE 0.08; 95% CI 0.71-1). Xpert® Carba-R may be considered an additional diagnostic tool for early diagnosis of carbapenem resistance in abdominal septic patients. Clinicians should be aware of their epidemiology before its introduction in the diagnostic protocol of their intensive care units.
- Published
- 2016
40. Presence of Class I Integrons in Multidrug-Resistant, Low-Prevalence Salmonella Serotypes, Italy
- Author
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Nastasi, Antonino and Mammina, Caterina
- Subjects
Salmonella -- Research ,Drug resistance in microorganisms -- Research ,Cellular control mechanisms -- Research ,Salmonellosis -- Drug therapy - Abstract
In 1997 to 1999, we detected class I integrons in multidrug-resistant isolates of Salmonella enterica serovars Anatum, Blockley, Brandenburg, Bredeney, Derby, Heidelberg, Livingstone, Newport, Ohio, Panama, Paratyphi B, Saintpaul, Sandiego, [...]
- Published
- 2001
41. Antimicrobial Resistance in Salmonella Enteritidis, Southern Italy, 1990-1998
- Author
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Nastasi, Antonio, Mammina, Caterina, and Cannova, Lucia
- Subjects
Drug resistance in microorganisms -- Research ,Salmonella enteritidis -- Research ,Italy -- Health aspects - Abstract
During 1990 to 1998, we identified multidrug-resistant isolates of Salmonella Enteritidis in southern Italy. Plasmids containing class I integrons and codifying for synthesis of extended-spectrum [Beta]-lactamases were detected. Active surveillance [...]
- Published
- 2000
42. Typing methods used in the molecular epidemiology of microbial pathogens: a how-to guide
- Author
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Ranjbar, Reza, Karami, Ali, Farshad, Shohreh, Giovanni Giammanco, Mammina, Caterina, Ranjbar, R, Karami, A, Farshad, S, Giammanco, G, and Mammina, C
- Subjects
Molecular Typing ,Molecular Epidemiology ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,Bacteria ,Humans ,Molecular epidemiology, DNA-based typing, Bacterial pathogens ,Bacterial Infections ,Settore MED/42 - Igiene Generale E Applicata - Abstract
Microbial typing is often employed to determine the source and routes of infections, confirm or rule out outbreaks, trace cross-transmission of healthcare-associated pathogens, recognize virulent strains and evaluate the effectiveness of control measures. Conventional microbial typing methods have occasionally been useful in describing the epidemiology of infectious diseases. However, these methods are generally considered too variable, labour intensive and time-consuming to be of practical value in epidemiological investigations. Moreover, these approaches have proved to be insufficiently discriminatory and poorly reproducible. DNA-based typing methods rely on the analysis of the genetic material of a microorganism. In recent years, several methods have been introduced and developed for investigation of the molecular epidemiology of microbial pathogens. Each of them has advantages and limitations that make them useful in some studies and restrictive in others. The choice of a molecular typing method therefore will depend on the skill level and resources of the laboratory and the aim and scale of the investigation. This study reviews the most popular DNA-based molecular typing methods used in the epidemiology of bacterial pathogens together with their advantages and limitations.
- Published
- 2014
43. Use of Cepheid Xpert Carba-R® for Rapid Detection of Carbapenemase-Producing Bacteria in Abdominal Septic Patients Admitted to Intensive Care Unit
- Author
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Cortegiani, Andrea, primary, Russotto, Vincenzo, additional, Graziano, Giorgio, additional, Geraci, Daniela, additional, Saporito, Laura, additional, Cocorullo, Gianfranco, additional, Raineri, Santi Maurizio, additional, Mammina, Caterina, additional, and Giarratano, Antonino, additional
- Published
- 2016
- Full Text
- View/download PDF
44. Complete Genome Sequence of KPC-3- and CTX-M-15-Producing Klebsiella pneumoniae Sequence Type 307
- Author
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Villa, Laura, primary, Feudi, Claudia, additional, Fortini, Daniela, additional, Iacono, Michele, additional, Bonura, Celestino, additional, Endimiani, Andrea, additional, Mammina, Caterina, additional, and Carattoli, Alessandra, additional
- Published
- 2016
- Full Text
- View/download PDF
45. Design, Implementation, and Evaluation of the Adolescents and Surveillance System for the Obesity Prevention Project
- Author
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Tabacchi, Garden, primary, Bianco, Antonino, additional, Alessi, Nicola, additional, Filippi, Anna Rita, additional, Napoli, Giuseppe, additional, Jemni, Monèm, additional, Censi, Laura, additional, Breda, João, additional, Schumann, Nathali Lehmann, additional, Firenze, Alberto, additional, Vitale, Francesco, additional, and Mammina, Caterina, additional
- Published
- 2016
- Full Text
- View/download PDF
46. Double Copies of bla KPC-3 ::Tn 4401a on an IncX3 Plasmid in Klebsiella pneumoniae Successful Clone ST512 from Italy
- Author
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Fortini, Daniela, primary, Villa, Laura, additional, Feudi, Claudia, additional, Pires, João, additional, Bonura, Celestino, additional, Mammina, Caterina, additional, Endimiani, Andrea, additional, and Carattoli, Alessandra, additional
- Published
- 2016
- Full Text
- View/download PDF
47. Bloodstream infections in intensive care unit patients: distribution and antibiotic resistance of bacteria
- Author
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Russotto,Vincenzo, Cortegiani,Andrea, Graziano,Giorgio, Saporito,Laura, Raineri,Santi Maurizio, Mammina,Caterina, Giarratano,Antonino, Russotto,Vincenzo, Cortegiani,Andrea, Graziano,Giorgio, Saporito,Laura, Raineri,Santi Maurizio, Mammina,Caterina, and Giarratano,Antonino
- Abstract
Vincenzo Russotto,1 Andrea Cortegiani,1 Giorgio Graziano,2 Laura Saporito,2 Santi Maurizio Raineri,1 Caterina Mammina,2 Antonino Giarratano1 1Department of Biopathology and Medical Biotechnologies (DIBIMED), Section of Anaesthesia, Analgesia, Intensive Care and Emergency, Paolo Giaccone University Hospital, University of Palermo, Palermo, Italy; 2Department of Sciences for Health Promotion and Mother-Child Care, University of Palermo, Palermo, Italy Abstract: Bloodstream infections (BSIs) are among the leading infections in critically ill patients. The case-fatality rate associated with BSIs in patients admitted to intensive care units (ICUs) reaches 35%–50%. The emergence and diffusion of bacteria with resistance to antibiotics is a global health problem. Multidrug-resistant bacteria were detected in 50.7% of patients with BSIs in a recently published international observational study, with methicillin resistance detected in 48% of Staphylococcus aureus strains, carbapenem resistance detected in 69% of Acinetobacter spp., in 38% of Klebsiella pneumoniae, and in 37% of Pseudomonas spp. Prior hospitalization and antibiotic exposure have been identified as risk factors for infections caused by resistant bacteria in different studies. Patients with BSIs caused by resistant strains showed an increased risk of mortality, which may be explained by a higher incidence of inappropriate empirical therapy in different studies. The molecular genetic characterization of resistant bacteria allows the understanding of the most common mechanisms underlying their resistance and the adoption of surveillance measures. Knowledge of epidemiology, risk factors, mechanisms of resistance, and outcomes of BSIs caused by resistant bacteria may have a major influence on global management of ICU patients. The aim of this review is to provide the clinician an update on BSIs caused by resistant bacteria in ICU patients. Keywords: bloodstream infections, multidrug resistant, antibiotic
- Published
- 2015
48. High dose of trimethoprim-sulfamethoxazole and daptomycin as a therapeutic option for MRSA endocarditis with large vegetation complicated by embolic stroke: a case report and literature review
- Author
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DI CARLO, Paola, D'ALESSANDRO, Natale, GUADAGNINO, Giuliana, BONURA, Celestino, MAMMINA, Caterina, LUNETTA, Monica, NOVO, Salvatore, GIARRATANO, Antonino, Di Carlo, P, D'Alessandro, N, Guadagnino, G, Bonura, C, Mammina, C, Lunetta, M, Novo, S, and Giarratano, A
- Subjects
Settore MED/07 - Microbiologia E Microbiologia Clinica ,therapeutic option, MRSA, endocarditis - Published
- 2013
49. A WEB-BASED SURVEILLANCE SYSTEM ON ADOLESCENTS’ LIFESTYLES AND OBESITY PREVENTION: ASSO-FTB PRELIMINARY FINDINGS. THE ASSO PROJECT
- Author
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Thomas, Ewan, PATTI, Antonino, BIANCO, Antonino, MAMMINA, Caterina, TABACCHI, Garden, Raccuglia, M, Thomas, E, Raccuglia, M, Patti, A, Bianco, A, Mammina, C, and Tabacchi, G
- Subjects
adolescence, surveilance system, physical fitness, tests - Abstract
The ASSO Project (Adolescents and Surveillance System for Obesity prevention), financed by the Italian Ministry of Health, aims to develop a surveillance system structured on adolescents lifestyles. It is a prospective study with online data collection, simultaneous descriptive data analysis and real time report. The ASSO-Toolkit is made of questionnaires, forms and a fitness test battery. The aims of this study is to evaluate the predictive validity, the criterion validity and the reliability of ASSO-FTB, in other words to assess how well the system operates to meet its objectives. The ASSO-FTB consists of 20mSRT (Shuttle Run Test) to estimate cardiorespiratory fitness, the handgrip strength test and the standing broad jump to assess musculoskeletal fitness, the 4x10mSRT to assess motor fitness and last but not least the sit up test to estimate muscle endurance. The project is currently spreading in southern Italy, both in urban and rural territories. A comparison has been made between two schools to evaluate if demographic aspects affect adolescents fitness and ensure that the project itself is reliable and reproducible. Forty male adolescents (17,32 ± 1,0 yrs; 175,3 ± 6,0 cm; 72,4 ± 14,7 kg; waist circ. 86,2 ± 12,4 cm) attending an urban secondary school and forty-nine adolescents ( 15,86 ± 1,4 yrs; 169,8 ± 8,9 cm; 64,3 ± 15) attending a rural secondary school voluntarily participated to the study . In a school setting, the standardized procedures were administered by the ASSO-FTB specialist in collaboration with the (PE) teacher. All data collected were updated through the already existing website (www.assoproject.info) and codified by the dedicated ASSO-FTB server. Participants were significantly higher in BMI compared to data published by Espana-Romeo et al in 2010. As expected, the maximal isometric strength showed no differences between urban (35,07±7,3 kg) and rural (33,7±10,8 kg) school respectively. Data that remains in line with what published by Espana-Romeo et al in 2010. The standing broad jump (178,07 ± 26,9 cm) has evinced less distance than Espana-Romeo study (183,4 ± 32,75), p = 0,37. On the whole findings show that there is a correlation between between handgrip and the 4x10 meter shuttle run test (r=-0.7) and between handgrip and standing broad jump (r = 0.7) underlining that this last test is better indicative of the upper and lower body muscular strength. The only concern comes from the VO2 max results, showing a mean value of 24 ml/kg/min. It is still to determine if the 20m shuttle run test underestimates this parameter or if adolescents have at this stage of development a reduced cardiorespiratory fitness. The trial showed that the FTB-system is reliable and reproducible. At this stage ASSO-FTB seems to be able to meet scientific standards. More studies are necessary for the ASSO-toolkit validation. Preliminary results are very encouraging.
- Published
- 2013
50. Ventilator-associated pneumonia and MRSA ST398, Italy
- Author
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Mammina, Caterina, Cala, Cinzia, Plano, Maria R.A., Bonura, Celestino, Vella, Antonietta, Monastero, Rachele, and Palma, Daniela M.
- Subjects
Medical research -- Health aspects ,Medicine, Experimental -- Health aspects ,Staphylococcus aureus -- Health aspects ,Health - Abstract
To the Editor: Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST)398 has become increasingly common in livestock, particularly pigs, in some countries in Europe, such as Spain and Germany (1). In [...]
- Published
- 2010
- Full Text
- View/download PDF
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