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209 results on '"Malendowicz, Ludwik K."'

3. The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients

Author

Blatkiewicz, Małgorzata, primary, Kamiński, Kacper, additional, Szyszka, Marta, additional, Al-Shakarchi, Zaid, additional, Olechnowicz, Anna, additional, Stelcer, Ewelina, additional, Komarowska, Hanna, additional, Tyczewska, Marianna, additional, Klimont, Anna, additional, Karczewski, Marek, additional, Wierzbicki, Tomasz, additional, Mikołajczyk-Stecyna, Joanna, additional, Ruchała, Marek, additional, Malendowicz, Ludwik K., additional, and Ruciński, Marcin, additional

Published
2023
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6. Adropin Stimulates Proliferation and Inhibits Adrenocortical Steroidogenesis in the Human Adrenal Carcinoma (HAC15) Cell Line

Author

Stelcer, Ewelina, primary, Milecka, Paulina, additional, Komarowska, Hanna, additional, Jopek, Karol, additional, Tyczewska, Marianna, additional, Szyszka, Marta, additional, Lesniczak, Marta, additional, Suchorska, Wiktoria, additional, Bekova, Karlygash, additional, Szczepaniak, Beata, additional, Ruchala, Marek, additional, Karczewski, Marek, additional, Wierzbicki, Tomasz, additional, Szaflarski, Witold, additional, Malendowicz, Ludwik K., additional, and Rucinski, Marcin, additional

Published
2020
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10. Analysis of Transcriptome, Selected Intracellular Signaling Pathways, Proliferation and Apoptosis of LNCaP Cells Exposed to High Leptin Concentrations

Author

Szyszka, Marta, primary, Paschke, Lukasz, additional, Tyczewska, Marianna, additional, Jopek, Karol, additional, Celichowski, Piotr, additional, Milecka, Paulina, additional, Sultanova, Gulnara, additional, Stelcer, Ewelina, additional, Malinska, Agnieszka, additional, Malendowicz, Ludwik K., additional, and Rucinski, Marcin, additional

Published
2019
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14. ZFP91 zinc finger protein expression pattern in normal tissues and cancers.

Author

Paschke, Lukasz, Jopek, Karol, Szyszka, Marta, Tyczewska, Marianna, Malendowicz, Ludwik K., and Rucinski, Marcin

Subjects
ZINC-finger proteins, TESTICULAR cancer, PROTEIN expression, PROTEIN microarrays, PROSTATE cancer
Abstract

Zinc finger protein 91 (ZFP91) gene has been recently acknowledged to possess oncogenic properties. To date, its expression has been examined only in a handful of human organs and cancer types. The aim of the present study was to characterize, for the first time, the ZFP91 expression pattern in a range of human tissues and cancer types. ZFP91 mRNA expression was examined using Cancer Survey cDNA sets. Utilized cDNA samples represented 15 human organs and 17 cancer types. ZFP91 mRNA expression was the highest in the testes and lymph nodes. It was downregulated in testis cancer, lymphoma and thyroid cancer, and upregulated in prostate cancer. Among the analyzed cancer types, ZFP91 expression was markedly elevated in sarcomas and melanoma. On a protein level, a large-scale reverse phase protein array was employed providing samples from 11 organ types and from cancers derived from these organs. ZFP91 protein expression was revealed to be generally stable across the tested samples and was only moderately elevated in breast, ovarian and pancreatic cancers. To the best of our knowledge, this is the first study to thoroughly analyze the ZFP91 expression pattern in human tissues and cancers. The obtained results provide the foundation for further work aiming to reveal its full biological significance. [ABSTRACT FROM AUTHOR]

Published
2019

17. Transcriptome Profile in Unilateral Adrenalectomy-Induced Compensatory Adrenal Growth in the Rat.

Author

Jopek, Karol, Tyczewska, Marianna, Celichowski, Piotr, Malendowicz, Ludwik K., and Rucinski, Marcin

Subjects
ADRENALECTOMY, ADRENOCORTICAL hormones, TRANSCRIPTOMES, LABORATORY rats, HYDROCORTISONE
Abstract

Compensatory adrenal growth evoked by unilateral adrenalectomy (hemiadrenalectomy) constitutes one of the most frequently studied in vivo models of adrenocortical enlargement. This type of growth has been quite well characterized for its morphological, biochemical, and morphometric parameters. However, the molecular basis of compensatory adrenal growth is poorly understood. Therefore, the aim of this study was to investigate the rat adrenal transcriptome profile during the time of two previously described adrenocortical proliferation waves at 24 and 72 h after unilateral adrenalectomy. Surgical removal of the left adrenal or a sham operation was accomplished via the classic dorsal approach. As expected, the weight of the remaining right adrenal glands collected at 24 and 72 h after hemiadrenalectomy increased significantly. The transcriptome profile was identified by means of Affymetrix® Rat Gene 2.1 ST Array. The general profiles of differentially expressed genes were visualized as volcano plots and heatmaps. Detailed analyzes consisted of identifying significantly enriched gene ontological groups relevant to adrenal physiology, by means of DAVID and GOplot bioinformatics tools. The results of our studies showed that compensatory adrenal growth induced by unilateral adrenalectomy exerts a limited influence on the global transcriptome profile of the rat adrenal gland; nevertheless, it leads to significant changes in the expression of key genes regulating the circadian rhythm. Our results confirm also that regulation of compensatory adrenal growth is under complex and multifactorial control with a pivotal role of neural regulatory mechanisms and a supportive role of other components. [ABSTRACT FROM AUTHOR]

Published
2018
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20. Mitochondrial sirtuins in the rat adrenal gland: location within the glands of males and females, hormonal and developmental regulation of gene expressions.

Author

Celichowski, Piotr, Jopek, Karol, Szyszka, Marta, Tyczewska, Marianna, Malendowicz, Ludwik K., and Rucinski, Marcin

Subjects
ADRENAL cortex, ADRENAL medulla, ADRENOCORTICOTROPIC hormone, ANIMAL experimentation, CELL culture, GENE expression, HYDROLASES, MITOCHONDRIA, RATS, TRANSFERASES, PHYSIOLOGY
Abstract

Introduction. Sirtuins are NAD dependent class III histone deacetylases. In adrenal cortex mitochondria are able to transform — via nicotinamide nucleotide transhydrogenase (NNT) — NAD into NADPH, which is required for steroidogenesis. These findings suggest that sirtuins expressed in mitochondria, Sirt3, Sirt4 and Sirt5, may be associated with adrenal steroidogenesis. Therefore, the purpose of this study was to characterize the expression of mitochondrial sirtuins (Sirt3–5) in individual compartments of rat adrenal cortex, their developmental regulation and to demonstrate whether their expression is dependent on adrenocorticotrophic hormone (ACTH) and Nampt (nicotinamide phosphoribosyltransferase also known as visfatin/PBEF), the rate-limiting enzyme in the regulation of mammalian NAD synthesis. Material and methods. Studies were performed on rat adrenal glands or on primary culture of rat adrenocortical cells. Expression of mitochondrial sirtuins (Sirt3–5) was evaluated by Affymetrix microarray system or QPCR. The bulk of data were extracted from our earlier experiments which have been reanalyzed in regard to Sirt3–5 mRNAs expression levels and — if necessary — validated by QPCR. Results. Sirt3–5 were expressed throughout the rat adrenal, with the highest expression level of Sirt5. The level of expression of all sirtuins is higher in the zona glomerulosa (ZG) and zona fasciculata/reticularis (ZF/R) than in the adrenal medulla. Sirt3 and Sirt5 expression levels were similar in adult male and female rats, while Sirt4 expression level was higher in females. As revealed by analysis of the available open database, no significant changes in Sirt3–5 expression levels in whole adrenal glands were observed up to week 104 of life of both male and female rats. Moreover, 60 min after intraperitoneal ACTH injection the expression level of Sirt3 in the en­tire gland was elevated while Sirt5 expression level lowered. On the other hand, chronic ACTH infusion (48 h) did not change expression of studied sirtuins. In cultured cells, ACTH greatly increased the expression levels of the Sirt4 and Sirt5. In cultured cells, Fk866 — a highly specific competitive inhibitor of Nampt — reduced expression level of Sirt5 only. In enucleation-induced regenerating rat adrenal, the expression levels of all studied sirtuins were significantly reduced in relation to the control group. Finally, in primary rat adrenal culture the FCS depletion elevates the Sirt3 and Sirt4 expression levels and downregulates Sirt5 expression. Conclusions. Sirt3–5 are expressed throughout the rat adrenal, with the highest expression levels in adrenal cortex. Performed experiments (ACTH stimulation, FCS depletion, regeneration) suggest that in the adrenal cortex, the mitochondrial Sirt5 is the primary mitochondrial sirtuin involved in regulating the biological activity of adrenocortical cells. Our results also suggest that normal levels of intracellular Nampt (iNampt) enzymatic activity are required to maintain normal (control) levels of Sirt5 mRNA in cultured cells. [ABSTRACT FROM AUTHOR]

Published
2017
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25. QRFP induces aldosterone production via PKC and T-type calcium channel-mediated pathways in human adrenocortical cells: evidence for a novel role of GPR103

Author

Ramanjaneya, Manjunath, primary, Karteris, Emmanouil, additional, Chen, Jing, additional, Rucinski, Marcin, additional, Ziolkowska, Agnieszka, additional, Ahmed, Naima, additional, Kagerer, Sonja, additional, Jöhren, Olaf, additional, Lehnert, Hendrik, additional, Malendowicz, Ludwik K., additional, and Randeva, Harpal S., additional

Published
2013
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28. Spexin Expression in Normal Rat Tissues

Author

Porzionato, Andrea, primary, Rucinski, Marcin, additional, Macchi, Veronica, additional, Stecco, Carla, additional, Malendowicz, Ludwik K., additional, and De Caro, Raffaele, additional

Published
2010
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29. Endocrine Disruptors and the Thyroid Gland—A Combinedin Vitroandin VivoAnalysis of Potential New Biomarkers

Author

Schmutzler, Cornelia, primary, Gotthardt, Inka, additional, Hofmann, Peter J., additional, Radovic, Branislav, additional, Kovacs, Gabor, additional, Stemmler, Luise, additional, Nobis, Inga, additional, Bacinski, Anja, additional, Mentrup, Birgit, additional, Ambrugger, Petra, additional, Grüters, Annette, additional, Malendowicz, Ludwik K., additional, Christoffel, Julie, additional, Jarry, Hubertus, additional, Seidlovà-Wuttke, Dana, additional, Wuttke, Wolfgang, additional, and Köhrle, Josef, additional

Published
2007
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30. Lack of expression of preproorexin and orexin receptors genes in human normal and prostate cancer cell lines.

Author

Szyszka, Marta, Paschke, Lukasz, Tyczewska, Marianna, Rucinski, Marcin, Grabowska, Paulina, and Malendowicz, Ludwik K.

Subjects
PROSTATE, ADRENAL glands, APPETITE stimulants, CELL culture, CELL physiology, DNA, ELECTROPHORESIS, GENE expression, GENES, PLACENTA, POLYMERASE chain reaction, PROSTATE tumors, DIAGNOSIS, ANATOMY
Abstract

Studies on expression of orexins (OXs) and their receptors in human prostate gland and human prostatic cell lines are scanty and their results contradictory. Regarding this, we carefully reinvestigated this problem on human prostatic cell lines.~Introduction~Background~Expression of preproorexin (ppOX) (6 primer pairs), and orexin receptors 1 and 2 (OXR1, OXR2) (4 and 2 primer pairs, respectively) was assessed by conventional PCR and QPCR in human normal (PrEC, PrSc, PrSmC) and prostate carcinoma (Du145, LNCaP, and PC3) cell lines. We designed intron spanning primers and also we applied primers from earlier publications and commercially available ones.~Material and Methods~Methods~With the designed primer pairs, in all studied cell lines we failed to demonstrate expression of ppOX, OXR1 and OXR2 genes at the mRNA level, while reaction products were observed in control tissues (human placenta and adrenals). Primers applied in earlier studies did not form amplification products specific for preproorexin or orexin 1 receptor. Some commercially available primers for orexin receptor 1 produced false positive results.~Results~Results~We found no evidence for the presence of preproorexin-orexin receptors system genes' mRNAs in human prostate cell lines. The reported premises for these genes¿ expression in prostate and prostatic cell lines may have arisen either from the presence of non-prostate cells included in the samples or from faulty PCR settings.~Conclusions~Conclusions [ABSTRACT FROM AUTHOR]

Published
2015
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