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Introduction: Energy status can alter thyroid hormone signalling in different tissues. Little is known about the effect of fasting on the local thyroid hormone metabolism under high-fat diet (HFD)-induced obesity. We aimed to investigate the fasting effect on deiodinase type 3 (DIO3) and thyroid hormone receptors (TRs) expression in liver and visceral adipose tissue (VAT) of HFD-induced obese mice. Methods: The 30 male C57BL/6 mice were divided into three groups (n = 10/group): control (CON) group, obese (OB) group, and fasted obese (OBF) group. Materials: In a 14-week study, the expression levels of DIO3 and TRs in the liver and VAT of mice were measured by real-time polymerase chain reaction. Gene expression results were shown as fold changes defined by 2−ΔΔct. Comparison between groups was performed by using one-way-ANOVA or Kruskal-Wallis ANOVA test. Results: In the liver, there was a significantly lower expression of DIO3 and higher expression of TRs in obese fasted mice compared to obese mice. Compared to the lean mice, OBF mice had significantly lower expression of DIO3 and higher expression of TRβ. In the VAT, mRNA expression of DIO3 was significantly increased in OBF and OB groups compared to the CON group. There were no significant differences in the mRNA expression of TRs between groups. Conclusion: Our findings suggest that fasting may be more effective in improving thyroid hormone metabolism in the liver rather than the VAT of obese mice.

Tramadol is a utilized medication globally that is included in numerous medical guidelines for the treatment of both short-term and longterm pain. There is an increasing body of research indicating the prevalence of tramadol abuse in certain Middle East nations. Tramadol exhibits certain adverse effects. The purpose of the present investigation is examination and treatment of the histopathological reaction that may develop in the spleen following tramadol administration. The mice were treated via a dose of tramadol by daily injection for 20 days and 40 days. Additionally, groups of mice were administered tramadol for 40 days, followed by daily treatment with either distilled water, Lagenaria siceraria (L.), or melatonin (MT) for another 40 days.The administration of tramadol significantly impacted the quantity of collagen materials present in the spleen cells. As a result, modifications were observed in the typical histological structure of the spleen.Ultimately, we propose that while tramadol has various adverse effects on the spleen tissues of male mice, the use of L. and MT treatments exhibits therapeutic benefits in alleviating these tramadol-induced side effects. [ABSTRACT FROM AUTHOR]

Background: Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits, and agricultural crops, its persistence in soils and crops could cause a health hazard to humans as well as other non-target organisms. Aim: This study was conducted to evaluate the effect of the recommended dose and its double of DM on sex hormones, sperm morphology, and fertility of adult male mice. Methods: Twenty-seven Swiss albino adult male mice were divided into three groups of nine animals each: control group received distilled water only, while other groups received DM orally at doses (0.1 and 0.2 ml DM/100 ml distilled water) for 20 days, at the end of the treatment, six mice from each group were sacrificed. The sperm morphology was evaluated and sex hormones were measured. Three mice from each group were allowed to mate with untreated females (1:2). Result: The results revealed a decrease in luteinizing hormone levels in mice treated with (0.2 ml DM/100 ml distilled water) compared with the control group while the levels of follicle-stimulating hormone and testosterone did not record any significant differences. Also, the results demonstrated a significant increase in abnormal sperm morphology such as head and tail. The fertility was reduced and the average number of dead embryos increased while the average number of live embryos decreased. Conclusion: This current study confirmed that DM has detrimental effects on sperm morphology, fertility, and the embryos; therefore, more efforts should be exerted to protect ourselves and our environment from the harmful effects of this pesticide. [ABSTRACT FROM AUTHOR]

Peganum harmala is one of the most used plants for the treatment of many diseases. Its effective compounds have pharmaceutical and medicinal properties. This study aims to determine the effect of aqueous extract of harmala plant seeds on body and liver weight, aspartate aminotransferase (AST), alanine transaminase (ALT), and the histological structure of liver of mice, as well as the size of the fetuses sired by treated mice. Sixteen adult male mice were divided into two groups of eight. The first group (control) was given distilled water orally, while the second group received the aqueous extract of harmala seeds at a dose of 300 mg/kg bw for three weeks. three untreated females were housed with one treated male for mating. At the end of the treatment, six male mice of each group were weighed and killed. Liver was extracted, weighed and its enzymes were measured. Also, sections of the liver were prepared for histological examination. The results showed a decrease in the body weight of the treated mice and a significant increase in the average weight of the fetuses compared with the control group, as well as marked changes in the hepatic tissue structure. There was no impact of the extract on fetal body length, liver weight and hepatic enzymes (AST and ALT) of treated mice. Further studies should be conducted to determine a safe dose that does not affect any organ in the body, so that it can be used for the treatment of many diseases. [ABSTRACT FROM AUTHOR]

Peganum harmala is one of the most used plants for the treatment of many diseases. Its effective compounds have pharmaceutical and medicinal properties. This study aims to determine the effect of aqueous extract of harmala plant seeds on body and liver weight, aspartate aminotransferase (AST), alanine transaminase (ALT), and the histological structure of liver of mice, as well as the size of the fetuses sired by treated mice. Sixteen adult male mice were divided into two groups of eight. The first group (control) was given distilled water orally, while the second group received the aqueous extract of harmala seeds at a dose of 300 mg/kg bw for three weeks. three untreated females were housed with one treated male for mating. At the end of the treatment, six male mice of each group were weighed and killed. Liver was extracted, weighed and its enzymes were measured. Also, sections of the liver were prepared for histological examination. The results showed a decrease in the body weight of the treated mice and a significant increase in the average weight of the fetuses compared with the control group, as well as marked changes in the hepatic tissue structure. There was no impact of the extract on fetal body length, liver weight and hepatic enzymes (AST and ALT) of treated mice. Further studies should be conducted to determine a safe dose that does not affect any organ in the body, so that it can be used for the treatment of many diseases.

Background: Dimethoate (DM) is one of the most important organophosphate insecticides used for controlling many pests which affect vegetables, fruits and agricultural crops, its persistence in soils and crops could cause a health hazard to humans as well as other non-target organisms. Aim: This study was conducted to evaluate effect of the recommended dose and its double of dimethoate (DM) on sex hormones, sperm morphology and fertility of adult male mice. Methods: Twenty-seven Swiss albino adult male mice were divided into three groups of nine animals each: control group received distilled water only, while other groups received DM orally at doses (0.1 and 0.2 ml DM /100 ml distilled water) for 20 days, at the end of the treatment, six mice from each group were sacrificed. The sperm morphology was evaluated and sex hormones were measured. Three mice from each group were allowed to mate with untreated females (1: 2). Result: The results revealed a decrease in LH level in mice treated with (0.2 ml DM /100 ml distilled water) compared with control group while the levels of FSH and testosterone did not record any significant differences. Also, the results demonstrated a significant increase in abnormal sperm morphology such as head and tail. The fertility reduced and average number of dead embryos increased while the average number of live embryos decreased. Conclusion: This current study confirmed that DM has detrimental effects on sperm morphology, fertility and the embryos, therefore, more efforts should be exerted to protect ourselves and our environment from the harmful effects of this pesticide. [Open Vet J 2023; 13(7.000): 873-878]

Introduction: Origanum Vulgare (Oregano) is a plant rich in antioxidant compounds and has therapeutic effects. The aim of this study was to investigate the effects of Oregano alcoholic extract on complications of depression in reserpine-poisoned mice. Methods: In this experimental study, 42 male BALB/c mice were divided into 6 groups of 7, including control (normal saline), negative control (reserpine), fluoxetine (positive control), and three reserpine groups treated respectively with 100, 200 and 400 mg/kg of Oregano via the intraperitoneal route. Depression was evaluated using forced swim test. Brain and serum total antioxidant capacity and malondialdehyde level were also determined. The data were analyzed by SPSS statistical software and One-Way ANOVA test. The significant was shown with (p≥0.05). Results: Negative control group significantly increased immobility time and decreased swimming time compared to the control group in the forced swim test (p≥0.001) and the extract at levels of 200 and 400 mg/kg similar to fluoxetine significantly reduced the immobility and increased the swimming time (p≥0.001). Reserpine in the negative control group decreased the antioxidant capacity and increased the level of malondialdehyde in serum and brain compared to the control group (p≥0.001).Compared to the negative control group, the extract at the level of 200 mg/kg, similar to fluoxetine, increased the antioxidant capacity in the brain (p≥0.01) and decreased the level of malondialdehyde in the serum and brain (p≥0.001). In addition, the extract at level of 400 mg/kg similar to fluoxetine significantly increased the total antioxidant capacity and decreased the level of malondialdehyde in serum and brain (p≥0.001) compared to control group. Conclusion: Oregano seems to have significant potential in reducing depressive symptoms and reducing oxidative stress due to its active antioxidant compounds.

Simple Summary: In recent years, special attention has been paid to studies on the effect of various plants on reproduction using laboratory animals. It is known that one of the major problems in a couple's life is infertility and sperm dysfunction. Many species of plants in the Mediterranean area have been used in the food, pharmaceutical, and cosmetics industries, such as P. harmala, which represents one of several plants that constitute a source of natural substances. The results of this study indicate that the extract of P. harmala at a high concentration seems to have more of an effect on sexual behavior in male mice. In the current study, the high concentration of P. harmala alkaloid extract improved the sperm count in the treated mice in comparison with the control group. However, no difference was recorded in sperm motility in the treated groups. The results revealed that the membrane integrity of the spermatozoa seemed to be stable in the treated male. However, seminal fructose and testosterone concentrations slightly increased in all groups. Based on the results of this study, it seems that the extract of P. harmala growing in Algeria can affect some reproductive indices, such as the gonadosomatic index and sperm count. The study was designed to evaluate the effects of the total alkaloid extract of Algerian Peganum harmala seeds on sexual behavior and male reproductive function. After two weeks of acclimatization, the male mice were randomly divided into four groups (seven mice in each group). For 35 days, the extract was administered orally at dose levels of 6.25, 12.5, and 25 mg/kg body weight per day to the respective groups of male mice (n = 7) and normal saline daily to the control group. On day 28, sexual behavior parameters were recorded. At the end of the trial, reproductive organ weights, sperm quality, seminal fructose, and testosterone hormone levels were evaluated. The three treated groups were compared with the control using statistical variance analysis (one-way ANOVA, p < 0.05), followed by Tukey's test. The results of the groups treated with 12.5 and 6.25 mg/kg of P. harmala alkaloid revealed the MF and IF parameters to be the lowest compared to the control group (p < 0.05). However, the male mice treated with 25 mg/kg recorded the highest values. A low significant value of ML was observed in the group treated with 25 mg/kg of the total alkaloid extract of P. harmala compared to the control group (p < 0.01), while a rise was observed in the concentration group treated with 6.25 mg/kg. Regarding IL, the male mice treated with different concentrations of the total alkaloid extract of P. harmala recorded a higher time than the control group. Moreover, an increase in the gonadosomatic index was noticed in all groups compared to the control group. However, there was a significant (p < 0.01) decrease in the sperm counts of the groups treated with 12.5 mg/kg and 6.25 mg/kg. However, there was no significant difference in the motility, membrane integrity, and total antioxidant capacity of sperm cells compared to the control. The extract treatment also brought about a non-significant increase in fructose content of the seminal vesicle and serum testosterone level. The findings of this study demonstrate that the extract acts in a dose-dependent manner, and it has varying effects on the reproductive parameters of male mice. [ABSTRACT FROM AUTHOR]

Sodium nitrite (NaNO2) is a main colorant and food preservative in the food industry. Besides the variety of medicinal and industrial applications, toxicity for animals and humans is well documented after overexposure of nitrite. This study aimed to investigate the reproductive toxicity of sodium nitrite and its modulation by ascorbic acid as an antioxidant in male mice. 60 adult male albino mice were used and divided into (6) groups. Gp(I) control. Gp(II) administered 100 mg/kg/d of ascorbic acid. Gp(III & IV) were received (16 and 32 mg/kg/day) sodium nitrite. Gp(V and VI) received (16 and 32 mg/kg/d) NaNO2 and 100 mg/kg/d of ascorbic acid. Male mice were orally administered NaNO2 by gastric intubation for 35 days. The study demonstrated that ascorbic acid ameliorates the alterations induced by sodium nitrite in reproductive performance, gonadosomatic index, testicular weight, sperm morphology, motility and count and repairing in the recorded histopathological lesions in the testis tissues. It could be concluded that ascorbic acid treatment has ameliorative effects against sodium nitrite reproductive toxicity. [ABSTRACT FROM AUTHOR]

Abstract Background Monosodium glutamate is a widely used flavor enhancer/food additive in meat, fish, milk and cheese or vegetable origins. Our present study aimed to assess the effect of utilization monosodium glutamate on the cardiac, splenic, hepatic and renal organs in mice. Thirty animals were divided into three groups: control group; glutamate 10 treated groups and glutamate 15 treated group. The experimental period was two successive weeks. Results Oral administrations of monosodium glutamate for 10 days induced moderate histopathological changes in cardiac, splenic, hepatic and renal tissues and also resulted in significant increase of the nuclear factor-kB expression depend on time of administration as compared to the control group, while treatment with monosodium glutamate for 14 days resulted in severe histopathological changes as well as highly significant increase of the nuclear factor-kB expression as compared to the control group. Conclusions It could be concluded that oral administration of monosodium glutamate induced histopathological alterations, and cytotoxicity in cardiac, splenic, hepatic and renal tissues dependent on time.

Simple Summary: When male mice are kept in groups at animal facilities, aggressive interactions between cage mates are not uncommon. Systematically reviewing previous studies that explored the cause of male mice aggression, we found that studies were disparate, using several different strains, a diverse set of environmental enrichments and different ways of grouping and housing mice, as well as different ways to observe aggression. Understanding the cause of male mice aggression is difficult when researchers use different methods and study designs. Nevertheless, our results suggest that home cage aggression is best studied in home cage environments and not by introducing unfamiliar mice to each other in a novel environment. In addition, while we were able to provide recommendations on how to minimize aggression, our assessment was that there is no universal solution that could be used by all animal facilities. Instead, it is important to realize that aggression is complex and that animal facilities might have to try different possible solutions to find what works best under their specific conditions. Aggression among group-housed male mice is a major animal welfare concern often observed at animal facilities. Studies designed to understand the causes of male mice aggression have used different methodological approaches and have been heterogeneous, using different strains, environmental enrichments, housing conditions, group formations and durations. By conducting a systematic literature review based on 198 observed conclusions from 90 articles, we showed that the methodological approach used to study aggression was relevant for the outcome and suggested that home cage observations were better when studying home cage aggression than tests provoking aggression outside the home cage. The study further revealed that aggression is a complex problem; one solution will not be appropriate for all animal facilities and all research projects. Recommendations were provided on promising tools to minimize aggression, based on the results, which included what type of environmental enrichments could be appropriate and which strains of male mice were less likely to be aggressive. [ABSTRACT FROM AUTHOR]

Background: Monosodium glutamate is a widely used flavor enhancer/food additive in meat, fish, milk and cheese or vegetable origins. Our present study aimed to assess the effect of utilization monosodium glutamate on the cardiac, splenic, hepatic and renal organs in mice. Thirty animals were divided into three groups: control group; glutamate 10 treated groups and glutamate 15 treated group. The experimental period was two successive weeks. Results: Oral administrations of monosodium glutamate for 10 days induced moderate histopathological changes in cardiac, splenic, hepatic and renal tissues and also resulted in significant increase of the nuclear factor-kB expression depend on time of administration as compared to the control group, while treatment with monosodium glutamate for 14 days resulted in severe histopathological changes as well as highly significant increase of the nuclear factor-kB expression as compared to the control group. Conclusions: It could be concluded that oral administration of monosodium glutamate induced histopathological alterations, and cytotoxicity in cardiac, splenic, hepatic and renal tissues dependent on time. [ABSTRACT FROM AUTHOR]

Traditional medicine has been long used to prevent and/or treat diseases worldwide based on a wealth of experiences, principles, and beliefs in different cultures. Among the extracts used in traditional medicine, we can refer to the aqueous beetle cocoon extract of Larinus maculates popularly known as Tihan in Iraq. To determine the histological effect of this extract on mice liver tissue, the animals were assigned to four groups (n=5) treated with (150,200,400) mg/kg of Cocoon aqueous extract of Larinus maculates for 14 days, respectively. Histology and immunohistochemistry test was performed to evaluate changes in liver tissue and tumor necrosis factor alpha (TNF-α) levels.The results showed various pathological dose-depended changes in the liver tissue, including infiltrations, congestion, and vacuolation, along with some dead cells; moreover, necrotic hepatocytes were observed in the liver of highly concentrated treatment group (400 mg kg-1). In addition, the TNF-α level in the liver tissue was elevated by the increased concentration of the extract. The immunohistochemistry result of positive reaction to TNF-α revealed high reaction in the liver tissue of mice treated with 200 mg kg-1 and 400 mg kg-1, as compared to the control group. Furthermore, as evidenced by the obtained results, changes in hepatocytes and the severity of pathological changes in the liver depends on the concentration of the extract of Larinus maculates.

The study was designed to evaluate the effects of the total alkaloid extract of Algerian Peganum harmala seeds on sexual behavior and male reproductive function. After two weeks of acclimatization, the male mice were randomly divided into four groups (seven mice in each group). For 35 days, the extract was administered orally at dose levels of 6.25, 12.5, and 25 mg/kg body weight per day to the respective groups of male mice (n = 7) and normal saline daily to the control group. On day 28, sexual behavior parameters were recorded. At the end of the trial, reproductive organ weights, sperm quality, seminal fructose, and testosterone hormone levels were evaluated. The three treated groups were compared with the control using statistical variance analysis (one-way ANOVA, p < 0.05), followed by Tukey’s test. The results of the groups treated with 12.5 and 6.25 mg/kg of P. harmala alkaloid revealed the MF and IF parameters to be the lowest compared to the control group (p < 0.05). However, the male mice treated with 25 mg/kg recorded the highest values. A low significant value of ML was observed in the group treated with 25 mg/kg of the total alkaloid extract of P. harmala compared to the control group (p < 0.01), while a rise was observed in the concentration group treated with 6.25 mg/kg. Regarding IL, the male mice treated with different concentrations of the total alkaloid extract of P. harmala recorded a higher time than the control group. Moreover, an increase in the gonadosomatic index was noticed in all groups compared to the control group. However, there was a significant (p < 0.01) decrease in the sperm counts of the groups treated with 12.5 mg/kg and 6.25 mg/kg. However, there was no significant difference in the motility, membrane integrity, and total antioxidant capacity of sperm cells compared to the control. The extract treatment also brought about a non-significant increase in fructose content of the seminal vesicle and serum testosterone level. The findings of this study demonstrate that the extract acts in a dose-dependent manner, and it has varying effects on the reproductive parameters of male mice.

Aims: the study of the biological effects of tramadol on testes of the male mice and treatment of its side effects via Lagenaria Siceraria or melatonin Results: the results of the present work showed the injection of tramadol for 20 and 40 days decreased the volume of the somniferous tubules and the number and volume of nuclei of the interstitial cells of Leydig in the testes of the male mice. While stopping tramadol injection, Lagenaria Siceraria or melatonin increased the diameter of the somniferous tubules and the number and volume of nuclei of the interstitial cells of Leydig in the testes of the male mice. The present data showed the injection of tramadol for 20 and 40 days lead to a lot of the pathological features and alternate the histological architectures of testes the male mice. While stopping tramadol injection, Lagenaria Siceraria or melatonin treats the histopathological side effects of tramadol. Conclusion: tramadol atrophy of the seminiferous tubules, and reduces the number and inhibits the cellular activities of the interstitial cells of Leydig and directly inhibits its function such as the production of testosterone hormone. Finally, tramadol inhibits germinal layer cells and the production of different reproductive cells. While stopping tramadol, Lagenaria Siceraria, or melatonin teats these tramadol effects. [ABSTRACT FROM AUTHOR]

Monosodium glutamate (MSG) is often added in foods to enhance the flavor. It has adverse effect to body organs. Natural remedies, such as: gambier have been known for generations used to improve health. Substances contained in gambier, i.e.: catechins are believed to reduce the occurrence of hepatotoxicity. The study aims to analyze the effects of gambier in reducing the hepatotoxicity of MSG. This study with a posttest only control group design. Mice amount 25 (5 groups, n = 5/group). All interventions are given orally for 4 weeks. At the end of the study, it carried out euthanasia taken the liver of mice to made histopathology preparations then examine by light microscope, ×400, ×5 view field. Liver damage was found in each group with various levels of histological appearance:(I) 2 normal, 2 minimal, 1 moderate and none severe; (II) none normal, 1 minimal, 3 moderate and 1 severe; (III) 1 normal, 4 minimal, none moderate and severe;, (IV) 2 normal, 3 minimal, none moderate and severe; and (V) 4 normal, 1 minimal, none moderate, and severe. The data were analyzed using Kruskal–Wallis test. The level of liver damage among all groups was significantly different (p = 0.017). The same on the Dunn test also showed the level of liver damage in Group-II to compare with other groups (p < 0.05). The present study proves that Gambier (Uncaria gambier Roxb) can reduce occurrence of hepatotoxicity caused by MSG.

Abstract Background Circadian rhythm disruption impacts a wide range of physiological processes, including fertility. However, the effect of circadian disruption on male spermatogenesis and fertility, and treatments for these effects have been largely unexplored at the molecular level. Methods In this study, we examined the effects of genipin on improving the reproductive health problems caused by circadian disruption. Three groups of animals were fed under different conditions: control group (normal T cycle with saline), group of shortened T cycles (Light/Dark = 4 hours/4 hours) with saline, and a group of shortened T cycles with genipin by oral gavage. The male fertility was evaluated by fertility study and pups parameters analysis after successful sexual behavior and mating with female mice. We sacrificed the treated animals after 5 or 10 weeks and collected the testis, sperm and serum for histological analysis, sperm motility assay, and serum hormone detection, respectively. Furthermore, the effect of genipin was assessed by detection of progesterone secretion and steroidogenic key proteins expression, including StAR and CYP11A1, in mouse Leydig tumor MLTC-1 cells. Results Male mice exposed to shortened light-dark cycles, much shorter than 24 hours, had reduced fertility with decreased sperm concentrations and sperm motility. Male mice under circadian disruption have reduced testis size and abnormal morphology, leading to lower fertility rates, reduced litter size and pup body weight. Treatment with exogenous genipin, a natural plant-derived compound, alleviated circadian disruption-induced damage to fertility and spermatogenesis and normalized testosterone, dihydrotestosterone (DHT), and androstenedione (ASD) levels in the male mice. The levels of key proteins involved in steroidogenesis, StAR and CYP11A1, were reduced in mouse testes after the circadian disruption, but genipin treatment restored the reduction. The mRNA expression of SRD5A1, which encodes an androgen synthesis enzyme, was also upregulated by genipin treatment. Furthermore, genipin treatment showed a positive effect on steroidogenesis in MLTC-1 cells, resulting in an increase in hormone secretion and the upregulation of StAR and CYP11A1. Conclusions Our results showed an association between circadian disruption and reproductive health problems in male mice and indicated that treatments with genipin have positive effects on the reproductive health of male mice with circadian rhythm disorders.

Getah virus (GETV) is a zoonotic arbovirus that can cause infection in many animals. It can cause pyrexia and reproductive losses in animals. The objective of the study was to explore the effects of GETV on male reproductive ability. Male mice were injected with 100 × TCID50/0.1 ml in a volume of 100-μL GETV in their hindquarter muscle, resulting in decreased semen quality and testicular histopathological changes, and the virus was detected in the testes. At 0.5 dpi (day post-infection), male mice showed decreased sperm density, motility, and decreased serum testosterone concentration, an increased sperm malformation rate, vacuoles in spermatogonial cells/spermatocytes in spermatogenic tubules, and the highest virus copies in testis. At 2 dpi, the sperm density and motility reached the lowest value of 3.99 × 106/ml and 62.03%, and the malformation rate reached 43.67%. At 28 dpi, the sperm indexes of the experimental group gradually approached that of the control group, but there were still significant differences. Since then, histopathological changes have worsened, with the most severe histopathological changes at 7 dpi and gradual recovery. Up to 14 dpi, the virus was detected by qRT-PCR and immunohistochemistry, which showed that the virus was only present in the testicular interstitium. GETV infection can rapidly enter the testis of mice and reduce the semen quality of mice, which needs to be paid attention to in the prevention and control of GETV.

Abstract Background Nicotine, a pharmacologically active component of tobacco adversely affects the male reproductive system and fertility whereas icariin (ICA), the main active ingredient in Epimedium herba has been used in the treatment of several male reproductive problems. This study aimed at evaluating the protective or ameliorative effect of ICA against reproductive toxicity induced by intraperitoneal injection of nicotine in mice. Methods Using simple random allocation, forty male mice were randomly divided into 4 groups: control (received 0.35 mL physiological saline via gastric gavage), nicotine (0.75 mg/kg BW/day intraperitoneally), ICA (75 mg/kg BW/day gastric gavage), and nicotine plus ICA (nicotine, 0.75 mg/kg BW/day intraperitoneally + ICA, 75 mg/kg BW/day gastric gavage) group. After 35 days of treatment, the mice were weighed, sacrificed, and their reproductive organs (testis and epididymis) were collected and examined for further studies. Results The nicotine-treated group showed significantly decreased epididymal sperm density and serum testosterone concentration relative to the control group. Nicotine also caused oxidative damage shown by significant reduction in the activities of antioxidant enzymes and elevation in Malondialdehyde (MDA) levels. ICA on the other hand, improved the reduction in sperm density, hormone levels, and activities of antioxidant enzymes altered in the nicotine treated mice. Conclusions These findings indicate that nicotine-induced reproductive toxicity and oxidative damage on male reproductive tissues could be attenuated by ICA.

Aggression among group-housed male mice is a major animal welfare concern often observed at animal facilities. Studies designed to understand the causes of male mice aggression have used different methodological approaches and have been heterogeneous, using different strains, environmental enrichments, housing conditions, group formations and durations. By conducting a systematic literature review based on 198 observed conclusions from 90 articles, we showed that the methodological approach used to study aggression was relevant for the outcome and suggested that home cage observations were better when studying home cage aggression than tests provoking aggression outside the home cage. The study further revealed that aggression is a complex problem; one solution will not be appropriate for all animal facilities and all research projects. Recommendations were provided on promising tools to minimize aggression, based on the results, which included what type of environmental enrichments could be appropriate and which strains of male mice were less likely to be aggressive.

In recent years, the use of plant-mediated nanoparticle synthesis to combat infectious diseases has become increasingly significant. Malaria is one of the world's most infectious diseases caused by Plasmodium species. The antioxidant, anti-apoptotic, and anti-inflammatory properties of nanosilver biosynthesized from Indigofera oblongifolia leaf extracts (NS) against Plasmodium chabaudi infection of the mouse liver were investigated in this research. Male mice were infected with P. chabaudi infected erythrocytes then treated with NS for 7 days. The parasitemia was suppressed by approximately 24, 28, 47 and 75% on days 4, 5, 6 and 7 postinfection, respectively after treatment of mice with NS. Also, NS was able to regulate the leucocytes count and the I L1β and TNF-α -mRNA expression in mice. Ns could increase the antioxidant activity in liver of mice and was able to regulate the apoptotic genes, Bcl2 and Casp3. We showed that NS has antioxidant, anti-apoptotic, and anti-inflammatory properties when it was used to treat the livers of mice infected with P. chabaudi. [ABSTRACT FROM AUTHOR]

Traditional medicine has been long used to prevent and/or treat diseases worldwide based on a wealth of experiences, principles, and beliefs in different cultures. Among the extracts used in traditional medicine, we can refer to the aqueous beetle cocoon extract of Larinus maculates popularly known as Tihan in Iraq. To determine the histological effect of this extract on mice liver tissue, the animals were assigned to four groups (n=5) treated with (150,200,400) mg/kg of Cocoon aqueous extract of Larinus maculates for 14 days, respectively. Histology and immunohistochemistry test was performed to evaluate changes in liver tissue and tumor necrosis factor alpha (TNF-α) levels.The results showed various pathological dose-depended changes in the liver tissue, including infiltrations, congestion, and vacuolation, along with some dead cells; moreover, necrotic hepatocytes were observed in the liver of highly concentrated treatment group (400 mg kg-1). In addition, the TNF-α level in the liver tissue was elevated by the increased concentration of the extract. The immunohistochemistry result of positive reaction to TNF-α revealed high reaction in the liver tissue of mice treated with 200 mg kg-1 and 400 mg kg-1, as compared to the control group. Furthermore, as evidenced by the obtained results, changes in hepatocytes and the severity of pathological changes in the liver depends on the concentration of the extract of Larinus maculates. [ABSTRACT FROM AUTHOR]

Background and Aim: Some medicinal plants are conventionally used for treatment of different aspects of male infertility. This study was conducted to evaluate the effects of Hibiscus Sabdariffa aqueous extract on spermatogenesis and sperm parameters of mice. materials and methods: Forty-eight were divided into 4 groups with 3 replications and 3 animals in per replication and received doses of 0, 100, 200 and 300 mg/kg of Hibiscus Sabdariffa aqueous extract for 28 days. Levels of testosterone, LH, FSH, TAS, SOD, GPx, blood variables, testis weight, sperm abnormality and viability, motility and sperm concentration were evaluated. Results: Oral administration of Hibiscus Sabdariffa aqueous extract lowered levels of FSH and LH in comparison to control group (P

Background: Nowadays treatment and control of pain is still one of problematic cases and syntetic analgesic drugs have several side effects. Objective: The aim of this study is to investigate the antinociceptive effects and phytochemical analysis of Ferula persica with respect to side effects of chemical drugs. Methods: The extract of plant inflorescence was prepared by percolation method. HPLC was used for phytochemical studies. In order to nociception test, male mice were used in acetic acid, immersion and formalin test. Mice were divided into experimental, control and positive control groups (receiving morphine) in each test. Results: In phytochemical analysis, four flavonoids consist of Quercetin, Luteolin, Apigenin and Rutin were identified. In all 3 pain tests, the experimental group had a significant difference with control group (P0.05). Conclusion: Methanolic extract of Ferula persica has antinociceptive effects, probably due to its sesquiterpenes and flavonoids.

Monosodium glutamate (MSG) is often added in foods to enhance the flavor. It has adverse effect to body organs. Natural remedies, such as: gambier have been known for generations used to improve health. Substances contained in gambier, i.e.: catechins are believed to reduce the occurrence of hepatotoxicity. The study aims to analyze the effects of gambier in reducing the hepatotoxicity of MSG. This study with a posttest only control group design. Mice amount 25 (5 groups, n = 5/group). All interventions are given orally for 4 weeks. At the end of the study, it carried out euthanasia taken the liver of mice to made histopathology preparations then examine by light microscope, ×400, ×5 view field. Liver damage was found in each group with various levels of histological appearance:(I) 2 normal, 2 minimal, 1 moderate and none severe; (II) none normal, 1 minimal, 3 moderate and 1 severe; (III) 1 normal, 4 minimal, none moderate and severe;, (IV) 2 normal, 3 minimal, none moderate and severe; and (V) 4 normal, 1 minimal, none moderate, and severe. The data were analyzed using Kruskal–Wallis test. The level of liver damage among all groups was significantly different (p = 0.017). The same on the Dunn test also showed the level of liver damage in Group-II to compare with other groups (p < 0.05). The present study proves that Gambier (Uncaria gambier Roxb) can reduce occurrence of hepatotoxicity caused by MSG. [ABSTRACT FROM AUTHOR]

Introduction: Phthalates exposure is a major public health concern due to the accumulation in the environment and associated with levels of testosterone reduction, leading to adverse pregnancy outcomes. However, the relationship between phthalate-induced testosterone level decline and ferroptosis remains poorly defined., Objectives: Herein, we aimed to explore the mechanisms of phthalates-induced testosterone synthesis disorder and its relationship to ferroptosis., Methods: We conducted validated experiments in vivo male mice model and in vitro mouse Leydig TM3 cell line, followed by RNA sequencing and metabolomic analysis. We evaluated the levels of testosterone synthesis-associated enzymes and ferroptosis-related indicators by using qRT-PCR and Western blotting. Then, we analyzed the lipid peroxidation, ROS, Fe 2+ levels and glutathione system to confirm the occurrence of ferroptosis., Results: In the present study, we used di (2-ethylhexyl) phthalate (DEHP) to identify ferroptosis as the critical contributor to phthalate-induced testosterone level decline. It was demonstrated that DEHP caused glutathione metabolism and steroid synthesis disorders in Leydig cells. As the primary metabolite of DEHP, mono-2-ethylhexyl phthalate (MEHP) triggered testosterone synthesis disorder accompanied by a decrease in the expression of solute carri1er family 7 member 11 (SLC7A11) protein. Furthermore, MEHP synergistically induced ferroptosis with Erastin through the increase of intracellular and mitochondrial ROS, and lipid peroxidation production. Mechanistically, overexpression of SLC7A11 counteracts the synergistic effect of co-exposure to MEHP-Erastin., Conclusion: Our research results suggest that MEHP does not induce ferroptosis but synergizes Erastin-induced ferroptosis. These findings provide evidence for the role of ferroptosis in phthalates-induced testosterone synthesis disorder and point to SLC7A11 as a potential target for male reproductive diseases. This study established a correlation between ferroptosis and phthalates cytotoxicity, providing a novel view point for mitigating the issue of male reproductive disease and "The Global Plastic Toxicity Debt"., (Copyright © 2024. Production and hosting by Elsevier B.V.)

Introduction: Energy status can alter thyroid hormone signalling in different tissues. Little is known about the effect of fasting on the local thyroid hormone metabolism under high-fat diet (HFD)-induced obesity. We aimed to investigate the fasting effect on deiodinase type 3 (DIO3) and thyroid hormone receptors (TRs) expression in liver and visceral adipose tissue (VAT) of HFD-induced obese mice., Methods: The 30 male C57BL/6 mice were divided into three groups ( n = 10/group): control (CON) group, obese (OB) group, and fasted obese (OBF) group., Materials: In a 14-week study, the expression levels of DIO3 and TRs in the liver and VAT of mice were measured by real-time polymerase chain reaction. Gene expression results were shown as fold changes defined by 2 -ΔΔct. Comparison between groups was performed by using one-way-ANOVA or Kruskal-Wallis ANOVA test., Results: In the liver, there was a significantly lower expression of DIO3 and higher expression of TRs in obese fasted mice compared to obese mice. Compared to the lean mice, OBF mice had significantly lower expression of DIO3 and higher expression of TRβ. In the VAT, mRNA expression of DIO3 was significantly increased in OBF and OB groups compared to the CON group. There were no significant differences in the mRNA expression of TRs between groups., Conclusion: Our findings suggest that fasting may be more effective in improving thyroid hormone metabolism in the liver rather than the VAT of obese mice., Competing Interests: There are no conflicts of interest., (Copyright: © 2024 Indian Journal of Endocrinology and Metabolism.)

Background: Circadian rhythm disruption impacts a wide range of physiological processes, including fertility. However, the effect of circadian disruption on male spermatogenesis and fertility, and treatments for these effects have been largely unexplored at the molecular level. Methods: In this study, we examined the effects of genipin on improving the reproductive health problems caused by circadian disruption. Three groups of animals were fed under different conditions: control group (normal T cycle with saline), group of shortened T cycles (Light/Dark = 4 hours/4 hours) with saline, and a group of shortened T cycles with genipin by oral gavage. The male fertility was evaluated by fertility study and pups parameters analysis after successful sexual behavior and mating with female mice. We sacrificed the treated animals after 5 or 10 weeks and collected the testis, sperm and serum for histological analysis, sperm motility assay, and serum hormone detection, respectively. Furthermore, the effect of genipin was assessed by detection of progesterone secretion and steroidogenic key proteins expression, including StAR and CYP11A1, in mouse Leydig tumor MLTC-1 cells. Results: Male mice exposed to shortened light-dark cycles, much shorter than 24 hours, had reduced fertility with decreased sperm concentrations and sperm motility. Male mice under circadian disruption have reduced testis size and abnormal morphology, leading to lower fertility rates, reduced litter size and pup body weight. Treatment with exogenous genipin, a natural plant-derived compound, alleviated circadian disruption-induced damage to fertility and spermatogenesis and normalized testosterone, dihydrotestosterone (DHT), and androstenedione (ASD) levels in the male mice. The levels of key proteins involved in steroidogenesis, StAR and CYP11A1, were reduced in mouse testes after the circadian disruption, but genipin treatment restored the reduction. The mRNA expression of SRD5A1, which encodes an androgen synthesis enzyme, was also upregulated by genipin treatment. Furthermore, genipin treatment showed a positive effect on steroidogenesis in MLTC-1 cells, resulting in an increase in hormone secretion and the upregulation of StAR and CYP11A1. Conclusions: Our results showed an association between circadian disruption and reproductive health problems in male mice and indicated that treatments with genipin have positive effects on the reproductive health of male mice with circadian rhythm disorders. [ABSTRACT FROM AUTHOR]

Obesity, a chronic metabolic disorder, can affect male reproductive function. As a functional beverage, tea has many biological activities and potential in the treatment of obesity. However, its effects on male reproductive damage induced by obesity are not yet clear. In this study, a murine model of obesity was established by feeding with high-fat diet (HF). A total of 24 male mice were divided into four groups: normal diet (control), HF, HF supplemented with 5% green tea powder (HF+G), and HF supplemented with 5% black tea powder (HF+B). The results showed that the HF + B significantly reduced the mouse body weight gain and testicular coefficient and lowered the serum insulin and leptin levels compared with the HF group. The sperm malformation rate of mice in the HF group had a significant increase when compared with the control group, the HF + B group had a significant decrease compared with the HF group, and no difference from the control group. The HF + G and HF + B significantly increased testosterone levels in serum compared with the HF group. The testosterone production-related gene cytochrome P450 family 11 subfamily a member (CYP11A1) and cytochrome p450 family 17 subfamily a member 1 (CYP17A1) expressions in testis were significantly increased in the HF + G group compared with HF group. In addition, the HF + G and HF + B abolished the effects of HF on superoxide dismutase (SOD), malondialdehyde, and glutathione levels in testis and antioxidant-related gene expressions of XRCC1 and SOD1. Overall, our findings have provided evidence that black and green tea has a positive effect on reducing reproductive damage in a male murine model of obesity, and that black tea is more effective than green tea. [ABSTRACT FROM AUTHOR]

Background and Aim: Baneh (Pistacia Atlantica) is a valuable oily fruit that has gained considerable attention because of its pharmaceutical, nutritional and industrial applications. This study aimed to investigate the antinociceptive effect of rubbing baneh oil using formalin test during acute and chronic phases in male laboratory mice. Materials and Methods: This experimental study was conducted on 30 male mice. They were allocated into 5 groups including control (without treatment), rubbing vaseline, morphine (positive control), baneh oil, and baneh oil plus morphine. Before the formalin test, the animals were treated with rubbing baneh oil for six hours. Morphine (10 mg/kg) was injected with a single dose subcutaneously. Pain scores were obtained using formalin test by injecting 0.02 ml of 2.5% formalin into the animals’ right palm. Results: Rubbing baneh oil significantly reduced the pain in acute pain (P

The male mouse is underrepresented in research of the urinary tract due to the difficulty of transurethral catheterization. As a result, there is a lack of analysis of sex differences in urinary tract research. Here, we present a novel catheter design and technique that enables urethral catheterization of male mice for bladder inoculation. Our catheterization technique uses the resistance met at the level of the external urinary sphincter and prostate to guide the retraction, positioning, and advancement of the catheter into the urinary bladder. We have shown that this method can be used to reproducibly catheterize 12 male mice with minimal urogenital trauma but cannot be used as a cystometric technique. This method will facilitate the expansion of research into sex differences in various genitourinary conditions that require transurethral catheterization of mice. [ABSTRACT FROM AUTHOR]

Silver nanoparticles (Ag NPs) are invested in various sectors and are becoming more persistent in our ambient environment with potential risk on our health and the ecosystems. The current study aims to investigate the histological, histochemical and ultrastructural hepatic changes that might be induced by 10 nm silver nanomaterials. Male mice (BALB/C) were exposed for 35 injections of daily dose of 10 nm Ag NPs (2 mg/kg). Liver tissues were subjected to examination by light and electron microscopy for histological, histochemical and ultrastructural alterations. Exposure to Ag NPs induced Kupffer cells hyperplasia, sinusoidal dilatation, apoptosis, ground glass hepatocytes appearance, nuclear changes, inflammatory cells infiltration, hepatocytes degeneration and necrosis. In addition, 10 nm Ag NPs induced histochemical alterations mainly glycogen depletion with no hemosiderin precipitation. Moreover, these nanomaterials exhibited ultrastructure alterations including mitochondrial swelling and cristolysis, cytoplasmic vacuolation, apoptosis, multilammellar myelin figures formation and endoplasmic destruction and reduction. The findings revealed that Ag NPs can induce alterations in the hepatic tissues, the chemical components of the hepatocytes and in the ultrastructure of the liver. One may also conclude that small size Ag NPs, which are increasingly used in human products could cause various toxigenic responses to all hepatic tissue components. [ABSTRACT FROM AUTHOR]

Background: Nicotine, a pharmacologically active component of tobacco adversely affects the male reproductive system and fertility whereas icariin (ICA), the main active ingredient in Epimedium herba has been used in the treatment of several male reproductive problems. This study aimed at evaluating the protective or ameliorative effect of ICA against reproductive toxicity induced by intraperitoneal injection of nicotine in mice. Methods: Using simple random allocation, forty male mice were randomly divided into 4 groups: control (received 0.35 mL physiological saline via gastric gavage), nicotine (0.75 mg/kg BW/day intraperitoneally), ICA (75 mg/kg BW/day gastric gavage), and nicotine plus ICA (nicotine, 0.75 mg/kg BW/day intraperitoneally + ICA, 75 mg/kg BW/day gastric gavage) group. After 35 days of treatment, the mice were weighed, sacrificed, and their reproductive organs (testis and epididymis) were collected and examined for further studies. Results: The nicotine-treated group showed significantly decreased epididymal sperm density and serum testosterone concentration relative to the control group. Nicotine also caused oxidative damage shown by significant reduction in the activities of antioxidant enzymes and elevation in Malondialdehyde (MDA) levels. ICA on the other hand, improved the reduction in sperm density, hormone levels, and activities of antioxidant enzymes altered in the nicotine treated mice. Conclusions: These findings indicate that nicotine-induced reproductive toxicity and oxidative damage on male reproductive tissues could be attenuated by ICA. [ABSTRACT FROM AUTHOR]

The present study aimed to use some plants extracts, such as celery and garlic in reducing the toxicity of dosed lead in white mice. The study included several experiments: the first included the progressive concentrations of lead in addition to control group (distilled water). The second and the third group were given only three progressive concentrations of plants, in addition to the control group which included a concentration of 6 mg/kg lead nitrate with DW. The results of statistical analysis at the p-value <0.05 showed a strong effect of the extracts used to reduce the toxicity of lead in male mice. Hence we can use the plants in the current study to improve the fertility of living organism, including humans because of their antioxidants. [ABSTRACT FROM AUTHOR]

Background High-fat diet (HFD) is highly responsible for the development of excessive reactive oxygen species (ROS). ROS and low-density lipoprotein (LDL) then trigger macrophage activation to secrete interleukin-6 (IL-6). Single bulb garlic or in Indonesian called bawang lanang is traditional medicine that possesses strong antioxidant properties. This study aimed to evaluate the effect of single bulb garlic oil (SBGO) on ROS, IL-6, and lymphocyte density in HFD-fed male mice. Methods This was an experimental study on 24 male mice randomly subdivided into 6 groups: one group was fed a normal diet, whereas the remaining five groups were fed HFD for 45 days and were then treated with single bulb garlic oil 0 mg/kg, simvastatin 26 mg/kg BW, single bulb garlic oil 12.5 mg/kg BW, 25 mg/kg BW, and 50 mg/kg BW respectively for the next 35 days. At the end of treatment, the mice were dissected for collection of (i) serum in order to measure ROS and IL-6 levels using ELISA, (ii) aortas to measure IL-6 expression using immunohistochemistry-fluorescence (IHC-F) and to obtain lymphocytes from bone marrow and spleen which were then counted using a light microscope. Results Our results indicated that SBGO decreased the ROS level, IL-6 level, IL-6 expression, and lymphocyte density in HFD mice. SBGO 12.5 mg/kg BW is the optimum dose in our study to reduce inflammation in HFD male mice. Conclusion SBGO is useful to reduce inflammation and improve antioxidant imbalance, thus might be a potential antiatherogenic agent.

Sari. Asam metoksiasetat (MAA) merupakan salah satu metabolit dari dimetoksietil ftalat (DMEP) yaitu suatu senyawa yang banyak digunakan dalam pembuatan plastik sebagai pelentur (plasticizer). DMEP dapat luruh dari plastik dan bila masuk ke dalam tubuh manusia, zat ini diubah menjadi MAA yang dapat menyebabkan efek teratogenik dan toksik terhadap organ tubuh. Terutama organ reproduksi jantan. Untuk meneliti pengaruh MAA terhadap organ reproduksi jantan, digunakan mencit Swiss Webster umur 7 minggu, yang cliberi dosis 100, 150, 225, dan 300 mg/kg berat badan, secara gavage setiap hari selama 4 minggu berturut-turut, dengan satu hari istirahat pada tiap akhir minggu. Kelompok mencit kontrol hanya diberi akuabides sebagai pelarut MAA. Mencit dibunuh sehari setelah pemberian MAA terakhir. Pengamatan dilakukan terhadap berat testis, berat epididimis, dan berat vesikula seminalis; jumlah, motilitas, dan morfologi spermatozoa yang diperoleh dari epididimis kauda; struktur testis yang mencakup diameter, tebal epitel, dan penampilan tubulus seminiferus; jumlah sel spermatogenik, jumlah sel Sertoli, serta jumlah sel Leydig. Dibandingkan dengan kontrol, hasil pengamatan menuniukkan penurunan berat testis dan epididimis, tetapi tidak ada penurunan berat vesikula seminalis. Jumlah dan motilitas spermatozoa menjadi lebih rendah, sedangkan jumlah spermatozoa abnormal meningkat. Diameter dan tebal epitel tubulus seminiferus menurun, demikian pula persentase tubulus seminiferus normal (fase VII), sedangkan persenfase tubulus seminiferus abnormal meningkat. Pendedahan terhadap MAA menyebabkan berdegenerasinya sel-sel spermatogenik, terutama spermatosit pakhiten dans permatid, yang ditunjukkan oleh jumlahnya yang berkurang. Spermatogonium A dan spermatosit praleptoten paling tahan terhadap MAA. Jumlah sel Sertoli dan sel Leydig tidak menurun oleh perlakuan MAA meskipun tampak adanya gejala toksik pada sel Sertoli yang ditandai oleh vakuolisasi dalam sitoplasma. Dapat disimpulkan bahwa testis merupakan organ reproduksi yang paling sensitif terhadap pengaruh MAA yang mengakibatkan terganggunya spermatogenesis pada mencit Swiss Webster. Effects of Methoxyacetic Acid on the Reproductive Organs of Male Swiss Webster Mice (Mus Musculus) Abstract. Methoxyacetic acid (MAA) is one of the metabolites of dimethoxyethyl phthalate (DMEP), which is mainly used as plasticizer in the manufacture of plastics. DMEP could leach from plastics and by entering the body, it will be metabolized into MAA which is teratogenic and toxic to several organs, particularly male reproductive organs. To investigate the effects of MAA on the male reproductive organs, seven-week-old Swiss Webster mice were treated with MAA at the doses of 100, 150, 225, and 300 mg/kg body weight daily by gavage, within four consecutive weeks with one-day interruption in each weekend. Observation was performed on the weight of testis, epididymis and seminal vesicle; the number, motility, and morphology of the spermatozoa obtained from the caudal epididymist; the histological structure of testis, including diameter, epithelial thickness and the performance of seminiferous tubule; the number of spermatogenic cells, as well as the number of Sertoli and Leydig cells. Compared to control the result showed, that testis and epididymis, but not the seminal vesicle decreased in weight. The number and motility of spermatozoa decreased, whereas the abnormal ones increased. The diameter, the epithelial thickness, as well as the percentage of normal (stage VII) seminiferous tubules were reduced, while the percentage of abnormal tubules increased. The administration of MAA led to the degeneration of spermatogenic cells, particularly the pachytene spermatocyte and spermatids, shown by the reduction in their number. The spermatogonia A and the preleptotene spermatocytes were the most resistant spermatogenic cells to MAA. The number of Sertoli and Leydig cells were not affected by MAA, despite toxic phenomenon of Sertoli cells characterized by vacuolization in the cytoplasm was shown. It is concluded that testis is the most susceptible to MAA insult, which subsequently interferes the spermatogenesis of male Swiss Webster mice.

Steroids, providing information regarding several biological patterns including stress and sexual behavior, have been investigated in different matrices in laboratory mice. Data regarding hair quantification, indicative of longer timespans when compared to blood and saliva, are lacking. The aim of the work was to analyze the hormonal hair profile of laboratory male mice and to investigate potential relationships with age and housing, as a potential tool for welfare assessment. Fifty-six adult male C57BL/6J and C57BL/6OlaHsd substrain mice were included in the study, housed in pairs or groups. Testosterone (T) and dehydroepiandrosterone (DHEA) were quantified by radioimmunoassay, corticosterone (CORT) by ELISA. Mean hormone levels were 6.42 pg/mg for T, 23.16 pg/mg for DHEA and 502.1 pg/mg for CORT. Age influenced all hormones by significantly increasing T and DHEA levels and decreasing CORT; only DHEA, significantly higher in grouped mice, was influenced by housing conditions. The influence of age indicates the need for accurate age-related reference intervals, while the higher levels of DHEA in grouped animals suggests that such housing practice may be beneficial for social interactions. In conclusion, it seems that hair hormones quantification may be a good tool for welfare assessment in laboratory mice and may help in refining husbandry.

Introduction: Epilepsy is a central nervous system disorder that appears as sudden convulsions, and transient, repetitive and unpredictable movement, with sensory-motor and autonomic sources. Statins are a group of blood cholesterol-lowering drugs. According to the results of the anti-inflammatory and vasoprotective effects of statins on cultured brain cells (astrocytes and microglia) and endothelial cells, the purpose of this study was investigating the effect of atorvastatin on pentilentetrazole-induced seizures in gonadectomized male mice. Methods: 36 male NMRI mice, weighting 30-35 g were used in the study. The mice were divided into two groups (Each group consisted of 18 mice : healthy and gonadectomized groups. The surgical removal of the testicles was gonadectomy and injections were given intraperitoneally. In gonadectomized group, an operation to remove the testicles was done. The DMSO group (0/3 cc) and treatment group were received Atorvastatin (10 mg/kg) intraperitoneally and then 30 minutes after that they received PTZ (80 mg/kg) injection. All injections were administered intraperitoneally. After injection of PTZ- duration of seizure onset (threshold) was recorded. The data were analyzed by one way variance analysis and Tukey’s test using SPSS (p

Formalin is a dangerous food preservative which has been banned to be used. Formalin is often used because it is relatively cheap and simple to use. This study aimed to analyze the effect of formalin to damage liver histology of male mice. Mice used were male mice Balb/C strain aged 4 weeks and weighted 20 to 22 grams. The extract of formalin-added-tofu, which were the concentration of: 0.1%, 1.68%, and 3.35%, 5.1%, 6.7%, were given orally per day for 12 weeks. At the end of 12th week, the mice were dissected and the liver were taken which then made preparations. The liver histological preparations were observed under a dino lite microscope in five different visual field, with a magnification of 800 times. For each of field of views, 20 cells were randomly counted. The average of weighted score of histopathological liver of five field of views then were randomly calculated for each mice. The scoring model used was Histopathology Manja Roenigk. The total of damage percentage occurred was recorded and calculated. The results showed that oral exposure to formaldehyde increases the amount of damage significantly in male mice strain Balb/C. The research results were presented in the form of booklets as a means of communication to the public media to broaden the danger of formalin-added-tofu. The booklet was made in order to perform the role of education as an agent of society renewal insight and knowledge. In addition, it also creating a continuous learning.

Objective: To assess the adverse effect of Deltamethrin (DM) (technical grade) on reproductive organs and fertility indices of male Swiss albino mice, Mus musculus. Methods: Forty male mice were divided into four experimental groups: control, vehicle control (peanut oil), high dose DM treated and low dose DM treated group. An oral dose of 3 mg/kg b.wt (low dose) and 6 mg/kg b.wt (high dose) of Deltamethrin was administered for a period of 45 days to male Swiss albino mice. Results: DM caused a significant reduction in body and organ weights, sperm count, sperm motility percent, sperm viability, serum testosterone level, sialic acid content of cauda epididymis and fructose level of seminal vesicle. DM-treated groups also showed a significant decline in testicular 3β and 17β Hydroxysteroid Dehydrogenase (HSD) activities. Histological examinations revealed significant alterations in the testes of dosed groups. Conclusion: Deltamethrin is a toxic pyrethroid pesticide that produced significant reproductive toxicity in treated male mice as revealed by the severely affected parameters and the altered gravimetric indices.

Background: Aspermia caused by exogenous testosterone limit its usage in late-onset hypogonadism (LOH) patients desiring fertility. Saikokaryukotsuboreito (SKRBT) is reported to improve serum testosterone and relieve LOH-related symptoms. However, it is unclear whether SKRBT affects fertility. We aimed to examine the effects of SKRBT on spermatogenesis and fertility in aging male mice. Methods: Thirty aging male mice were randomly assigned to three groups. Mice were orally administered with phosphate-buffer solution or SKRBT (300 mg/kg, daily) or received testosterone by subcutaneous injections (10 mg/kg, every 3 days). Thirty days later, each male mouse was mated with two female mice. All animals were sacrificed at the end of 90 days. Intratesticular testosterone (ITT) levels, quality of sperm, expression of synaptonemal complex protein 3 (SYCP3), and fertility were assayed. Results: In the SKRBT-treated group, ITT, quality of sperm, and expression of SYCP3 were all improved compared with the control group (ITT: 85.50 ± 12.31 ng/g vs. 74.10 ± 11.45 ng/g, P = 0.027; sperm number: [14.94 ± 4.63] × 106 cells/ml vs. [8.79 ± 4.38] × 106 cells/ml, P = 0.002; sperm motility: 43.16 ± 9.93% vs. 33.51 ± 6.98%, P = 0.015; the number of SYCP3-positive cells/tubule: 77.50 ± 11.01 ng/ml vs. 49.30 ± 8.73 ng/ml, P < 0.001; the expression of SYCP3 protein: 1.23 ± 0.09 vs. 0.84 ± 0.10, P < 0.001), but fertility was not significantly changed (P > 0.05, respectively). In the testosterone-treated group, ITT, quality of sperm, and expression of SYCP3 were markedly lower than the control group (ITT: 59.00 ± 8.67, P = 0.005; sperm number: [4.34 ± 2.45] × 106 cells/ml, P = 0.018; sperm motility: 19.53 ± 7.69%, P = 0.001; the number of SYCP3-positive cells/tubule: 30.00 ± 11.28, P < 0.001; the percentage of SYCP3-positive tubules/section 71.98 ± 8.88%, P = 0.001; the expression of SYCP3 protein: 0.71 ± 0.09, P < 0.001), and fertility was also suppressed (P < 0.05, respectively). Conclusion: SKRBT had no adverse effect on fertility potential in aging male mice.

The aim of this study was to evaluate the protective effect of different doses of diphenhydramin against acute toxicosiswith Levamisole. The Mechanism of levamisole induced acute toxicity and that of protective effect of diphenhydramin againstLevamisole toxicosis also examined on the level of cholinesterase (ChE) activity. Subcutanous injection of 100mg/kglevamisole in male mice with induced cholinergic over stimulation and death in 100% of animals. The Toxicosis was notrelated to the significantly decreased in plasma, red blood cells and brain ChE activity. Injection low dose of diphenhydramin2.5mg/kg S.C. 15 min before levamisole produced protective effect against acute toxicity with levamisole. Significantlydecreased the severity of toxicosis and increased survival rates to 100%. Diphenhydramin at low dose alone or with acute doseof levamisole did not Produced Significantly inhibition in ChE activity.The data suggested that the toxic effect of Levamisolewas not related to inhibition of ChE. The low dose of diphenhydramin protected mice from Levamisole toxicity. The antidoataleffect of diphenhydramin not at the level of protection from ChE inhibition. There was no adverse interaction between twodrugs.

Introduction Aging is characterized by gradual impairment in all physiological functions. Increases in free radicals and changes in organ morphology occur with aging. The purpose of this study was to determine age-related changes in serum free radicals, body weight, organ weights, and relative organ weights in male mice. Methods An experimental animal study was performed on 25 male mice (Mus musculus), which were randomized into 5 groups according to age at termination, i.e. 12 (group K1), 24 (K2), 32 (K3), 40 (K4) and 48 weeks (K5), respectively. Retro-orbital venous blood was taken for examination of malondialdehyde (MDA) levels. After termination, liver, heart, kidneys, testes, brain, thymus and spleen were weighed using an analytical balance. ANOVA and Kruskal Wallis tests were used to analyze the data, with p0.05) (liver p=0.023, heart p=0.000, kidneys p=0.002, testes p=0.000, brain p=0.012 and spleen p=0.006). Significant changes in relative weight of brain (p=0.001) and spleen (p=0.049) were also found with age. Conclusion This study demonstrated increases in serum MDA levels, body weight, and weights of the liver, heart, kidneys, testes, brain and spleen with age. Peak increases in weights of kidneys and thymus were found earlier than those in MDA levels and weights of other organs.