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6. Contribution to the physiology ofTrigonellainfected withPeronospora trifoliorum

Author

Gopal, S., Maggon, K. K., Venkitasubramanian, T. A., and Mukerji, K. G.

Abstract

Some biochemical properties ofTrigonella foenum-groecuminfected withPeronospora trifoliorumhave been investigated. Qualitative changes were observed in lipids, coumarins, amino acids and indole compounds only. A quantitative study of the phosphate content was also carried out. Neutral lipids did not show any significant changes. In addition to the fourteen phospholipid bands, the infected leaves contained a new band corresponding in Rfto phosphatidic acid or polyglycerophosphate. Seven coumarins were present in both the extracts. In addition 3-hydroxy coumarin, 5-hydroxy-4-methylcoumarin and 7-hydroxy-4-methyl-coumarin were detected in infected leaves. Higher levels of indole acetic acid and three of its derivatives were observed due to infection. Infection altered the free amino acid content. Tryptophan appeared and the total phosphate content increased. Increase in phenols and indoles suggest a higher respiratory rate whereas a higher content of inorganic phosphate suggests uncoupling of phosphorylation from respiration.

Published
1971
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15. Induction of mixed function oxidases on oral administration of dieldrin.

Author

Kohli KK, Maggon KK, and Venkitasubramanian TA

Subjects
Administration, Oral, Animals, Body Weight, Dieldrin administration & dosage, Enzyme Induction, Male, Microsomes, Liver drug effects, Microsomes, Liver enzymology, Phospholipids metabolism, Rats, Aflatoxins metabolism, Dieldrin pharmacology, Microsomes, Liver metabolism, Mixed Function Oxygenases biosynthesis, Oxidoreductases biosynthesis
Abstract

The administration of dieldrin (30 mg/kg body weight) caused an increase in the liver weight of rats. The metabolism of aflatoxins B1 and G1 by the microsomes obtained from the liver of dieldrin-treated animals was enhanced significantly as compared to the controls showing that dieldrin increased the activity of mixed function hydroxylases. Dieldrin caused an increase in the activity of liver microsomal NADPH oxidase and a decrease in the lipid peroxidation. Dieldrin brought about an increase in the phosphatidylcholine content of rat liver.

Published
1977
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