68 results on '"M. Stauber"'
Search Results
2. An Organometallic Gold(III) Reagent for
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James W, McDaniel, Julia M, Stauber, Evan A, Doud, Alexander M, Spokoyny, and Jennifer M, Murphy
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Fluorine Radioisotopes ,Isotope Labeling ,Gold ,Radiopharmaceuticals ,Peptides ,Sugars ,Article - Abstract
The (18)F-labeling of unprotected peptides and sugars with a Au(III)-[(18)F]fluoroaryl complex is reported. The chemoselective method generates (18)F-labeled S-aryl bioconjugates in an aqueous environment in 15 min with high radiochemical yields and displays excellent functional group tolerance. This approach utilizes an air and moisture stable, robust organometallic Au(III) complex and highlights the versatility of designer organometallic reagents as efficient agents for rapid radiolabeling.
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- 2023
3. Programmable synthesis of well-defined, glycosylated iron(ii) supramolecular assemblies with multivalent protein-binding capabilities
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Jake H. Schwab, Jake B. Bailey, Milan Gembicky, and Julia M. Stauber
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Chemical Sciences ,General Chemistry ,Generic health relevance - Abstract
Multivalency plays a key role in achieving strong, yet reversible interactions in nature, and provides critical chemical organization in biological recognition processes. Chemists have taken an interest in designing multivalent synthetic assemblies to both better understand the underlying principles governing these interactions, and to build chemical tools that either enhance or prevent such recognition events from occurring in biology. Rationally tailoring synthetic strategies to achieve the high level of chemical control and tunability required to mimic these interactions, however, is challenging. Here, we introduce a systematic and modular synthetic approach to the design of well-defined molecular multivalent protein-binding constructs that allows for control over size, morphology, and valency. A series of supramolecular mono-, bi-, and tetrametallic Fe(ii) complexes featuring a precise display of peripheral saccharides was prepared through coordination-driven self-assembly from simple building blocks. The molecular assemblies are fully characterized, and we present the structural determination of one complex in the series. The mannose and maltose-appended assemblies display strong multivalent binding to model lectin, Concanavalin A (K d values in μM), where the strength of the binding is a direct consequence of the number of saccharide units decorating the molecular periphery. This versatile synthetic strategy provides chemical control while offering an easily accessible approach to examine important design principles governing structure-function relationships germane to biological recognition and binding properties.
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- 2023
4. Tuning counterion chemistry to reduce carrier localization in doped semiconducting carbon nanotube networks
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Tucker L. Murrey, Taylor J. Aubry, Omar Leon Ruiz, Kira A. Thurman, Klaus H. Eckstein, Evan A. Doud, Julia M. Stauber, Alexander M. Spokoyny, Benjamin J. Schwartz, Tobias Hertel, Jeffrey L. Blackburn, and Andrew J. Ferguson
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General Energy ,General Engineering ,General Physics and Astronomy ,General Materials Science ,General Chemistry - Published
- 2023
5. An Organometallic Strategy for Cysteine Borylation
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Mary A. Waddington, Alexander M. Spokoyny, Julia M. Stauber, Hayden R. Montgomery, Liban M. A. Saleh, Xin Zheng, Petr Král, and Elamar Hakim Moully
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Boron Compounds ,chemistry.chemical_classification ,Bioconjugation ,Molecular Structure ,Chemistry ,Peptide ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Combinatorial chemistry ,Borylation ,Article ,Catalysis ,0104 chemical sciences ,Residue (chemistry) ,Colloid and Surface Chemistry ,DARPin ,Organometallic Compounds ,Moiety ,Cysteine ,Chemoselectivity ,Platinum - Abstract
Synthetic bioconjugation at cysteine (Cys) residues in peptides and proteins has emerged as a powerful tool in chemistry. Soft nucleophilicity of the sulfur in Cys renders an exquisite chemoselectivity with which various functional groups can be placed onto this residue under benign conditions. While a variety of reactions have been successful at producing Cys-based bioconjugates, the majority of these feature sulfur-carbon bonds. We report Cys-borylation, wherein a benchtop stable Pt(II)-based organometallic reagent can be used to transfer a boron-rich cluster onto a sulfur moiety in unprotected peptides forging a boron-sulfur bond. Cys-borylation proceeds at room temperature and tolerates a variety of functional groups present in complex polypeptides. Further, the bioconjugation strategy can be applied to a model protein modification of Cys-containing DARPin (designed ankyrin repeat protein). The resultant bioconjugates show no additional toxicity compared to their Cys alkyl-based congeners. Finally, we demonstrate how the developed Cys-borylation can enhance the proteolytic stability of the resultant peptide bioconjugates while maintaining the binding affinity to a protein target.
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- 2021
6. Dynamic Nuclear Polarization Using 3D Aromatic Boron Cluster Radicals
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Rebecca M. Kubena, Julia M. Stauber, Christian Hilty, Hamidreza Samouei, Pierce Pham, Alexander M. Spokoyny, Yaewon Kim, and Jonathan C. Axtell
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010304 chemical physics ,Chemistry ,Radical ,Dodecaborate ,chemistry.chemical_element ,Nuclear magnetic resonance spectroscopy ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Article ,0104 chemical sciences ,Delocalized electron ,0103 physical sciences ,Cluster (physics) ,General Materials Science ,Lewis acids and bases ,Physical and Theoretical Chemistry ,Boron ,Dissolution - Abstract
A set of two dodecaborate [B(12)(OR)(12)](1−) radical cluster anions containing a dense layer of fluorinated end-groups provide nuclear spin hyperpolarization via the dissolution dynamic nuclear polarization (D-DNP) technique. We show that these clusters can enhance (19)F nuclear magnetic resonance (NMR) signals. Importantly, given the inherent radical delocalization in dodecaborate-based radicals, these species are compatible with reactive compounds such as Lewis acids, providing ~ 1000 – 2000 times of signal enhancement for B(C(6)F(5))(3) in liquid state NMR experiments at 9.4 T. This observation suggests that 3D aromatic radicals can provide advantages over the conventional radical species that are currently used for DNP, such as 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) by showing superior chemical compatibility. The ability to hyperpolarize reactive compounds using [B(12)(OR)(12)](1−) cluster radicals opens up new applications of reaction monitoring by D-DNP NMR spectroscopy, including the observation of catalytically active species in complex reaction mixtures.
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- 2020
7. Electronic Structure of Superoxidized Radical Cationic Dodecaborate-Based Clusters
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Bo Li, Xinglong Zhang, Thomas F. Miller, Julia M. Stauber, and Alexander M. Spokoyny
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chemistry.chemical_classification ,Delocalized electron ,Partial charge ,chemistry.chemical_compound ,Computational chemistry ,Chemistry ,Aryl ,Dodecaborate ,Cationic polymerization ,Electronic structure ,Physical and Theoretical Chemistry ,Redox ,Alkyl - Abstract
The expanding field of boron clusters has attracted continuous theoretical efforts to understand their diverse structures and unique bonding. We recently discovered a new reversible redox event of B12(O-3-methylbutyl)12 in which the superoxidized radical cationic form [B12(O-3-methylbutyl)12]•+ was identified and isolated for the first time. Herein, comprehensive (TD-)DFT studies in tandem with electrochemical experiments were employed to demonstrate the generality of the reported behavior across perfunctionalized B12(OR)12 clusters (R = aryl or alkyl). While the spin density of radical cationic clusters is delocalized in the core region, the oxidation brings about notable gains of positive partial charges on the supporting groups whose electronics can readily tune the redox potential of the 0/•+ couple. The underlying changes of frontier orbitals were elucidated, and the resulting [B12(OR)12]•+ species manifest a general diagnostic absorption as a consequence of mixed local/charge-transfer excitations.
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- 2021
8. Cysteine Borylation in Unprotected Peptides
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Petr Král, Mary A. Waddington, Liban M. A. Saleh, Alexander M. Spokoyny, Julia M. Stauber, ElamarHakim Moully, and Alice Zheng
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chemistry.chemical_classification ,Residue (chemistry) ,chemistry.chemical_compound ,Bioconjugation ,Chemistry ,Aryl ,Moiety ,Peptide ,Chemoselectivity ,Combinatorial chemistry ,Borylation ,Cysteine - Abstract
Synthetic bioconjugation at cysteine (Cys) residues in peptides and proteins has emerged as a powerful tool in chemistry. Soft nucleophilicity of the sulfur in Cys renders an exquisite chemoselectivity with which various functional groups can be placed onto this residue under benign conditions. While a variety of reactions have been successful at producing Cys-based bioconjugates, the majority of these feature sulfur-carbon bonds. We report Cys-borylation, wherein a benchtop stable Pt(II)-based organometallic reagent can be used to transfer a boron-rich cluster onto a sulfur moiety in unprotected peptides forging a boron-sulfur bond. Discovered Cysborylation proceeds at room temperature and is tolerant to a variety of functional groups present in complex polypeptides. The resultant bioconjugates show no additional toxicity compared to their Cys aryl-based congeners. Finally, we demonstrate how the developed Cys-borylation can enhance the proteolytic stability of the produced peptide bioconjugates while maintaining the binding affinity to a protein target.
- Published
- 2021
9. Gold(III) Aryl Complexes as Reagents for Constructing Hybrid Peptide-Based Assemblies via Cysteine S-Arylation
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Julia M. Stauber, Alexander M. Spokoyny, and Arnold L. Rheingold
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chemistry.chemical_classification ,Bioconjugation ,Molecular Structure ,Bicyclic molecule ,Biomolecule ,Aryl ,Peptide ,Combinatorial chemistry ,Article ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Covalent bond ,Functional group ,Indicators and Reagents ,Cysteine ,Physical and Theoretical Chemistry ,Chemoselectivity ,Peptides ,Organogold Compounds - Abstract
Organometallic complexes have recently gained attention as competent bioconjugation reagents capable of introducing a diverse array of substrates to biomolecule substrates. Here, we detail the synthesis and characterization of an aminophosphine-supported Au(III) platform that provides rapid and convenient access to a wide array of peptide-based assemblies via cysteine S-arylation. This strategy results in the formation of robust C‒S covalent linkages and is an attractive method for the modification of complex biomolecules due to the high functional group tolerance, chemoselectivity, and rapid reaction kinetics associated with these arylation reactions. This work expands upon existing metal-mediated cysteine arylation by introducing a class of air-stable organometallic complexes that serve as competent bioconjugation reagents enabling the synthesis of conjugates of higher structural complexity including macrocyclic stapled and bicyclic peptides, as well as a peptide-functionalized multivalent hybrid nanocluster. This organometallic-based approach provides a convenient, one-step method of peptide functionalization and macrocyclization, and has the potential to contribute to efforts directed towards developing efficient synthetic strategies of building new and diverse hybrid peptide-based assemblies of high complexit
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- 2020
10. Tunable Dopants with Intrinsic Counterion Separation Reveal the Effects of Electron Affinity on Dopant Intercalation and Free Carrier Production in Sequentially Doped Conjugated Polymer Films
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Taylor J. Aubry, Victoria M. Basile, Charlene Z. Salamat, Sarah H. Tolbert, Benjamin J. Schwartz, Alexander M. Spokoyny, Matthew J. Bird, Minh Dinh Phan, K. J. Winchell, Julia M. Stauber, Rebecca M. Kubena, Jonathan C. Axtell, and Jeffrey Lindemuth
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inorganic chemicals ,Electron mobility ,Materials science ,02 engineering and technology ,010402 general chemistry ,Polaron ,01 natural sciences ,counterion distance ,Biomaterials ,Engineering ,redox‐driven infiltration ,Electron affinity ,molecular dopants ,Electrochemistry ,Materials ,tunable electron affinity ,redox-driven infiltration ,chemistry.chemical_classification ,Dopant ,doping efficiency ,electronic offset ,semiconducting polymers ,Doping ,Polymer ,Full Papers ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,chemistry ,Chemical physics ,Physical Sciences ,Chemical Sciences ,Neutron reflectometry ,Counterion ,0210 nano-technology - Abstract
Carrier mobility in doped conjugated polymers is limited by Coulomb interactions with dopant counterions. This complicates studying the effect of the dopant's oxidation potential on carrier generation because different dopants have different Coulomb interactions with polarons on the polymer backbone. Here, dodecaborane (DDB)‐based dopants are used, which electrostatically shield counterions from carriers and have tunable redox potentials at constant size and shape. DDB dopants produce mobile carriers due to spatial separation of the counterion, and those with greater energetic offsets produce more carriers. Neutron reflectometry indicates that dopant infiltration into conjugated polymer films is redox‐potential‐driven. Remarkably, X‐ray scattering shows that despite their large 2‐nm size, DDBs intercalate into the crystalline polymer lamellae like small molecules, indicating that this is the preferred location for dopants of any size. These findings elucidate why doping conjugated polymers usually produces integer, rather than partial charge transfer: dopant counterions effectively intercalate into the lamellae, far from the polarons on the polymer backbone. Finally, it is shown that the IR spectrum provides a simple way to determine polaron mobility. Overall, higher oxidation potentials lead to higher doping efficiencies, with values reaching 100% for driving forces sufficient to dope poorly crystalline regions of the film.
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- 2020
11. A Super-Oxidized Radical Cationic Icosahedral Boron Cluster
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Andrew J. Martinolich, Kimberly A. See, Paul H. Oyala, Julia M. Stauber, Thomas F. Miller, Dahee Jung, Brendon J. McNicholas, Harry B. Gray, Josef Schwan, Xinglong Zhang, Alexander M. Spokoyny, Jonathan C. Axtell, and Jay R. Winkler
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Icosahedral symmetry ,Chemistry ,Substitution (logic) ,Cationic polymerization ,chemistry.chemical_element ,General Chemistry ,Electrochemistry ,Redox ,Biochemistry ,Catalysis ,law.invention ,Delocalized electron ,chemistry.chemical_compound ,Crystallography ,Colloid and Surface Chemistry ,Unpaired electron ,Ferrocene ,Radical ion ,law ,Alkoxy group ,Cluster (physics) ,Electron paramagnetic resonance ,Boron - Abstract
While the icosahedral closo-[B12H12]2– cluster does not display reversible electrochemical behavior, perfunctionalization of this species via substitution of all twelve B–H vertices with alkoxy orbenzyloxy (OR) substituents engenders reversible redox chemistry, providing access to clusters in the dianionic,monoanionic, and neutral forms. Here, we evaluated the electrochemical behavior of the electron-rich B12(O-3-methylbutyl)12 (1) cluster and discovered that a new reversible redox event that gives rise to a fourth electronic state is accessible through one-electron oxidation of the neutral species. Chemical oxidation of 1 with [N(2,4-Br2C6H3)3]•+ afforded the isolable[1] •+ cluster, which is the first example of an open-shell cationic B12 cluster in which the unpaired electron is proposed to be delocalized throughout the boron cluster core. The oxidation of 1 is also chemically reversible, where treatment of [1]•+ with ferrocene resulted in its reduction back to 1. The identity of [1]•+ is supported by EPR, UV-vis, multinuclear NMR (1H, 11B), and X-ray photoelectron spectroscopic characterization.
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- 2020
12. Retraction of 'Pushing Single-Oxygen-Atom-Bridged Bimetallic Systems to the Right: A Cryptand-Encapsulated Co-O-Co Unit'
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Christopher C. Cummins, Laura Gagliardi, Julia M. Stauber, Shao-Liang Zheng, Dugan Hayes, Ryan G. Hadt, Eric D. Bloch, Lin X. Chen, Konstantinos D. Vogiatzis, and Daniel G. Nocera
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Absorption spectroscopy ,Ligand ,Cryptand ,General Chemistry ,Biochemistry ,Catalysis ,Ion ,Crystallography ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,chemistry ,Oxidation state ,Complete active space ,Bimetallic strip ,Potassium superoxide - Abstract
A dicobalt(II) complex, [Co2(mBDCA-5t)](2-) (1), demonstrates a cofacial arrangement of trigonal monopyramidal Co(II) ions with an inter-metal separation of 6.2710(6) A. Reaction of 1 with potassium superoxide generates an encapsulated Co-O-Co core in the dianionic complex, [Co2O(mBDCA-5t)](2-) (2); to form the linear Co-O-Co core, the inter-metal distance has diminished to 3.994(3) A. Co K-edge X-ray absorption spectroscopy data are consistent with a +2 oxidation state assignment for Co in both 1 and 2. Multireference complete active space calculations followed by second-order perturbation theory support this assignment, with hole equivalents residing on the bridging O-atom and on the cryptand ligand for the case of 2. Complex 2 acts as a 2-e(-) oxidant toward substrates including CO and H2, in both cases efficiently regenerating 1 in what represent net oxygen-atom-transfer reactions. This dicobalt system also functions as a catalase upon treatment with H2O2.
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- 2020
13. A Molecular Boron Cluster-Based Chromophore with Dual Emission
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PeterI. Djurovich, Gary J. Balaich, JustinR. Caram, Kierstyn P. Anderson, Mary A. Waddington, Julia M. Stauber, and Alexander M. Spokoyny
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chemistry.chemical_compound ,Electrophilic substitution ,chemistry ,Cyclohexane ,Molecule ,Borane ,Chromophore ,Luminescence ,Photochemistry ,Phosphorescence ,Fluorescence - Abstract
Bromination of the luminescent borane, anti-B18H22, via electrophilic substitution using AlCl3 and Br2 yields the monosubstituted derivative 4-Br-anti-B18H21 as an air-stable crystalline solid. In contrast to the unsubstituted parent compound, 4-Br-anti-B18H21 product possesses dual emission upon excitation with UV light and exhibits fluorescence at 410 nm and phosphorescence at 503 nm, with Фtotal = 0.07 in oxygen-free cyclohexane. Increased oxygen content in cyclohexane solution quenches the phosphorescence signal. The fluorescent signal intensity remains unaffected by oxygen, suggesting that this molecule could be used as a ratiometric oxygen probe.
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- 2020
14. A Molecular Boron Cluster-Based Chromophore with Dual Emission
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Alexander Spokoyny, Peter I. Djurovich, Justin R. Caram, Julia M. Stauber, Gary J. Balaich, Mary A. Waddington, and Kierstyn P. Anderson
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Bromination of the luminescent borane, anti-B18H22, via electrophilic substitution using AlCl3 and Br2 yields the monosubstituted derivative 4-Br-anti-B18H21 as an air-stable crystalline solid. In contrast to the unsubstituted parent compound, 4-Br-anti-B18H21 product possesses dual emission upon excitation with UV light and exhibits fluorescence at 410 nm and phosphorescence at 503 nm, with Фtotal = 0.07 in oxygen-free cyclohexane. Increased oxygen content in cyclohexane solution quenches the phosphorescence signal. The fluorescent signal intensity remains unaffected by oxygen, suggesting that this molecule could be used as a ratiometric oxygen probe.
- Published
- 2020
15. Second-Coordination-Sphere Assisted Selective Colorimetric Turn-on Fluoride Sensing by a Mono-Metallic Co(II) Hexacarboxamide Cryptand Complex
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Christopher C. Cummins, Julia M. Stauber, Glen E. Alliger, and Daniel G. Nocera
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Aqueous solution ,Coordination sphere ,010405 organic chemistry ,medicine.drug_class ,Cryptand ,Inorganic chemistry ,chemistry.chemical_element ,Carboxamide ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,Inorganic Chemistry ,Metal ,chemistry.chemical_compound ,chemistry ,visual_art ,medicine ,visual_art.visual_art_medium ,Physical and Theoretical Chemistry ,Cobalt ,Single crystal ,Fluoride - Abstract
The preparation of a selective turn-on colorimetric fluoride sensor was achieved through single cobalt(II) ion insertion into a macrobicyclic cryptand. Monometallic [Co(mBDCA-5t-H3)]− (1) and [Zn(mBDCA-5t-H3)]− (2) complexes were prepared in 74 and 84% yields, respectively. Structural characterization of 1 confirmed the presence of a proximal hydrogen-bonding network consisting of carboxamide N–H donors. The reaction of 1 with F– was accompanied by a distinct colorimetric turn-on response in mixed aqueous/organic media, and 1 was capable of selective fluoride sensing in the presence of large quantities of potentially competitive anions. Complex 1 represents a unique example of a fluoride sensor wherein selective F– binding takes place directly at a transition-metal center and induces a color change based upon metal-centered transitions. The metal(II) fluoride complexes [F⊂Co(mBDCA-5t-H3)]2– (3) and [F⊂Zn(mBDCA-5t-H3)]2– (4) were both fully characterized, including single crystal X-ray analyses. Fluoride b...
- Published
- 2017
16. An Organometallic Strategy for Assembling Atomically Precise Hybrid Nanomaterials
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Daishi Fujita, Arnold L. Rheingold, Yanxiao Han, Petr Král, Julia M. Stauber, Elaine A. Qian, and Alexander M. Spokoyny
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Spectrometry, Mass, Electrospray Ionization ,Magnetic Resonance Spectroscopy ,Materials science ,Metal Nanoparticles ,Nanotechnology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,Article ,Nanomaterials ,Nanoclusters ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Organometallic Compounds ,Cluster (physics) ,Systematic synthesis ,Organometallic chemistry ,Extramural ,General Chemistry ,0104 chemical sciences ,chemistry ,Complex protein ,Colloidal gold ,Gold ,Multivalent binding - Abstract
For decades, chemists have strived to mimic the intricate design and diverse functions of naturally occurring systems through the bioinspired synthesis of programmable inorganic nanomaterials. The development of thiol-capped gold nanoparticles (AuNPs) has driven advancement in this area; however, although versatile and readily accessible, hybrid AuNPs are rarely atomically precise, which limits control over their surface topology and therefore the study of complex structure-function relationships. Here, we present a bottom-up approach to the systematic assembly of atomically precise hybrid nanoclusters employing a strategy that mimics the synthetic ease with which thiol-capped AuNPs are normally constructed, while producing welldefined covalent nanoscale assemblies with diverse surface topologies. For the first time, using a structurally characterized cluster-based organometallic building block, we demonstrate the systematic synthesis of nanoclusters with multivalent binding capabilities to complex protein targets.
- Published
- 2019
17. Multi-electron reactivity of a cofacial di-tin(<scp>ii</scp>) cryptand: partial reduction of sulfur and selenium and reversible generation of S3˙−
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Peter Müller, Gang Wu, Yizhe Dai, Daniel G. Nocera, Julia M. Stauber, Christopher C. Cummins, Institute for Medical Engineering and Science, Massachusetts Institute of Technology. Department of Biology, Massachusetts Institute of Technology. Department of Chemical Engineering, Massachusetts Institute of Technology. Department of Chemistry, Stauber, Julia M., Miller, Peter G, and Nocera, Daniel G
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010405 organic chemistry ,Chemistry ,Cryptand ,Inorganic chemistry ,chemistry.chemical_element ,General Chemistry ,Electron ,010402 general chemistry ,01 natural sciences ,Medicinal chemistry ,Sulfur ,0104 chemical sciences ,Chalcogen ,SN2 reaction ,Tin ,Bimetallic strip ,Selenium - Abstract
Cofacial bimetallic tin( II ) ([Sn 2 ( m BDCA-5t)] 2-, 1) and lead( II ) ([Pb₂(mBDCA-5t)]²-, 2) complexes have been prepared by hexadeprotonation of hexacarboxamide cryptand m BDCA-5t-H₆ together with double Sn(II) or Pb(II) insertion. Reaction of 1 with elemental sulfur or selenium generates di-tin polychalcogenide complexes containing m -E and bridging m -E 5 ligands where E ¼ S or Se, and the Sn( II ) centers have both been oxidized to Sn( IV ). Solution and solid-state UV-Vis spectra of [( m -S 5 )Sn 2 ( m -S)( m BDCA-5t)] 2 ( 4 ) indicate that the complex acts reversibly as a source of S 3 c in DMF solution with a K eq ¼ 0.012 0.002. Reductive removal of all six chalcogen atoms is achieved through treatment of [( m -E 5 )Sn 2 ( m -E)( m BDCA- 5t)] 2 with PR 3 (R ¼ t Bu, Ph, O i Pr) to produce six equiv. of the corresponding EPR 3 compound with regeneration of di-tin( II ) cryptand complex 1., National Science Foundation (U.S.) (CHE-1305124)
- Published
- 2016
18. Cobalt and Vanadium Trimetaphosphate Polyanions: Synthesis, Characterization, and Electrochemical Evaluation for Non-aqueous Redox-Flow Battery Applications
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Julia M. Stauber, Shiyu Zhang, Keith J. Stevenson, Nataliya A. Gvozdik, Yanfeng Jiang, Laura Avena, and Christopher C. Cummins
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Aqueous solution ,chemistry.chemical_element ,Vanadium ,Infrared spectroscopy ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Biochemistry ,Redox ,Catalysis ,0104 chemical sciences ,Electrochemical cell ,Colloid and Surface Chemistry ,chemistry ,0210 nano-technology ,Cobalt ,Nuclear chemistry ,Monoclinic crystal system - Abstract
An electrochemical cell consisting of cobalt ([CoII/III(P3O9)2]4–/3–) and vanadium ([VIII/II(P3O9)2]3–/4–) bistrimetaphosphate complexes as catholyte and anolyte species, respectively, was constructed with a cell voltage of 2.4 V and Coulombic efficiencies >90% for up to 100 total cycles. The [Co(P3O9)2]4– (1) and [V(P3O9)2]3– (2) complexes have favorable properties for flow-battery applications, including reversible redox chemistry, high stability toward electrochemical cycling, and high solubility in MeCN (1.09 ± 0.02 M, [PPN]4[1]·2MeCN; 0.77 ± 0.06 M, [PPN]3[2]·DME). The [PPN]4[1]·2MeCN and [PPN]3[2]·DME salts were isolated as crystalline solids in 82 and 68% yields, respectively, and characterized by 31P NMR, UV/vis, ESI-MS(−), and IR spectroscopy. The [PPN]4[1]·2MeCN salt was also structurally characterized, crystallizing in the monoclinic P21/c space group. Treatment of 1 with [(p-BrC6H4)3N]+ allowed for isolation of the one-electron-oxidized spin-crossover (SCO) complex, [Co(P3O9)2]3– (3), which is...
- Published
- 2017
19. Spinal neuronal activation during locomotor-like activity enabled by epidural stimulation and 5-hydroxytryptamine agonists in spinal rats
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Trinh T. Pham, Roland R. Roy, Mei Si Xiao, Paul O. Duru, Hui Zhong, Niranjala J.K. Tillakaratne, Jung A. Kim, Stacey M. Stauber, and V. Reggie Edgerton
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Agonist ,education.field_of_study ,medicine.drug_class ,business.industry ,Quipazine ,education ,Population ,Stimulation ,Serotonergic ,Cellular and Molecular Neuroscience ,Lumbar Spinal Cord ,Lumbar ,medicine ,Cholinergic ,business ,Neuroscience ,medicine.drug - Abstract
The neural networks that generate stepping in complete spinal adult rats remain poorly defined. To address this problem we used c-fos (an activity-dependent marker) to identify active interneurons and motoneurons in the lumbar spinal cord of adult spinal rats during a 30-minute bout of bipedal stepping. Spinal rats were either step trained (30 min/day, 3 days/week for 7.5 weeks) or not step-trained. Stepping was enabled by epidural stimulation and the administration of the serotonergic agonists quipazine and 8-OHDPAT. A third group of spinal rats served as untreated (no stimulation, drugs, or stepping) controls. The number of activated cholinergic central canal cluster cells and partition neurons was higher in both step-trained and non-trained than untreated rats, and higher in non-trained than step-trained rats. The latter finding suggests that daily treatment with epidural stimulation plus serotonergic agonist treatment without step training enhanced the excitability of a broader cholinergic interneuronal population than step training. The number of activated interneurons in laminae II-VI of lumbar cross sections was higher in both step-trained and non-trained than untreated rats, and highest in step-trained rats. This finding suggests that this population of interneurons was responsive to epidural stimulation plus serotonergic treatment and that load-bearing induced when stepping had an additive effect. The number of activated motoneurons of all size categories was higher in the step-trained than the other two groups, reflecting a strong effect of loading on motoneuron recruitment. In general, these results indicate that the spinal networks for locomotion are similar with and without brain input.
- Published
- 2015
20. Terminal Titanyl Complexes of Tri- and Tetrametaphosphate: Synthesis, Structures, and Reactivity with Hydrogen Peroxide
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Julia M. Stauber and Christopher C. Cummins
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010405 organic chemistry ,Stereochemistry ,Metaphosphate ,Dimer ,Space group ,Triclinic crystal system ,010402 general chemistry ,01 natural sciences ,Medicinal chemistry ,0104 chemical sciences ,Inorganic Chemistry ,chemistry.chemical_compound ,chemistry ,Yield (chemistry) ,Reactivity (chemistry) ,Physical and Theoretical Chemistry ,Hydrogen peroxide ,Monoclinic crystal system - Abstract
The synthesis and characterization of tri- and tetrametaphosphate titanium(IV) oxo and peroxo complexes is described. Addition of 0.5 equiv of [OTi(acac)2]2 to dihydrogen tetrametaphosphate ([P4O12H2]2–) and monohydrogen trimetaphosphate ([P3O9H]2–) provided a bis(μ2,κ2,κ2) tetrametaphosphate titanyl dimer, [OTiP4O12]24– (1; 70% yield), and a trimetaphosphate titanyl acetylacetonate complex, [OTiP3O9(acac)]2– (2; 59% yield). Both 1 and 2 have been structurally characterized, crystallizing in the triclinic P1 and monoclinic P21 space groups, respectively. These complexes contain Ti≡O units with distances of 1.624(7) and 1.644(2) A, respectively, and represent rare examples of structurally characterized terminal titanyls within an all-oxygen coordination environment. Complexes 1 and 2 react with hydrogen peroxide to produce the corresponding peroxotitanium(IV) metaphosphate complexes [O2TiP4O12]24–(3; 61% yield) and [O2TiP3O9(acac)]2– (4; 65% yield), respectively. Both 3 and 4 have been characterized by si...
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- 2017
21. Raman spectroscopy and microspectrophotometry of reactive dyes on cotton fibres: Analysis and detection limits
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Z. Van Zanten, S. Chabli, Geneviève Massonnet, T. Sauneuf, C. Meile, T. Coyle, A. Blumer, Claude Roux, Steven E. J. Bell, M. Stauber, Louise A. Fido, Kenneth G. Wiggins, F. Monard, C. Smith, S. Ketterer, Avner Rosengarten, J. Hemmings, H. Leijenhorst, Patrick Buzzini, and G. Jochem
- Subjects
Detection limit ,Blue dye ,Chemistry ,Analytical chemistry ,Laser ,Pathology and Forensic Medicine ,law.invention ,symbols.namesake ,chemistry.chemical_compound ,Wavelength ,law ,Microscopy ,symbols ,Reactive dye ,Legal & Forensic Medicine ,Raman spectroscopy ,Law ,Chemical composition - Abstract
A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentration ranging from 0.5 to 0.005% (w/w).This survey shows that it is possible to detect the presence of dye in fibres with concentrations below that detectable by the traditional methods of light microscopy and microspectrophotometry (MSP). The MSP detection limit for the dyes used in this study was found to be a concentration of 0.5% (w/w). At this concentration, the fibres appear colourless with light microscopy. Raman spectroscopy clearly shows a higher potential to detect concentrations of dyes as low as 0.05% for the yellow dye RY145 and 0.005% for the blue dye RB221. This detection limit was found to depend both on the chemical composition of the dye itself and on the analytical conditions, particularly the laser wavelength.Furthermore, analysis of binary mixtures of dyes showed that while the minor dye was detected at 1.5% (w/w) (30% of the total dye concentration) using microspectrophotometry, it was detected at a level as low as 0.05% (w/w) (10% of the total dye concentration) using Raman spectroscopy.This work also highlights the importance of a flexible Raman instrument equipped with several lasers at different wavelengths for the analysis of dyed fibres. The operator and the set up of the analytical conditions are also of prime importance in order to obtain high quality spectra. Changing the laser wavelength is important to detect different dyes in a mixture. © 2012 Elsevier Ireland Ltd.
- Published
- 2012
22. Evaporation of droplets on strongly hydrophobic substrates
- Author
-
Stephen Wilson, Brian R. Duffy, Khellil Sefiane, and Jutta M. Stauber
- Subjects
FLOW ,Evaporation ,Analytical chemistry ,Nanoparticle ,DROPS ,Power law ,Contact angle ,QA273 ,NANOPARTICLES ,Electrochemistry ,General Materials Science ,WETTABILITY ,KINETICS ,Spectroscopy ,Range (particle radiation) ,Chemistry ,Surfaces and Interfaces ,ANGLE ,Condensed Matter Physics ,SOLID-SURFACES ,Volume (thermodynamics) ,Chemical physics ,SESSILE DROPLETS ,CONTACT LINE ,Wetting ,Constant (mathematics) - Abstract
The manner in which the extreme modes of droplet evaporation (namely, the constant contact radius and the constant contact angle modes) become indistinguishable on strongly hydrophobic substrates is described. Simple asymptotic expressions are obtained which provide good approximations to the evolutions of the contact radius, the contact angle, and the volume of droplets evaporating in the extreme modes for a wide range of hydrophobic substrates. As a consequence, on strongly hydrophobic substrates it is appropriate to use the so-called "2/3 power law" to extrapolate the lifetimes of droplets evaporating in the constant contact radius mode as well as in the constant contact angle mode.
- Published
- 2015
23. Improved anchorage in osteoporotic vertebrae with new implant designs
- Author
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Christian Knop, Berend Linke, Jörg Goldhahn, M. Reinhold, R. Frei, M. Stauber, and Erich Schneider
- Subjects
Male ,musculoskeletal diseases ,medicine.medical_specialty ,Bone Screws ,Osteoporosis ,Lumbar vertebrae ,Lumbar ,Bone Density ,Humans ,Medicine ,Cyclic loading ,Orthopedics and Sports Medicine ,Aged ,Aged, 80 and over ,Bone mineral ,Orthodontics ,Lumbar Vertebrae ,business.industry ,Prostheses and Implants ,medicine.disease ,Biomechanical Phenomena ,Surgery ,medicine.anatomical_structure ,Female ,Stress, Mechanical ,Implant ,business ,Bone structure - Abstract
The goal of our study was to evaluate two newly developed implant designs and their behavior in terms of subsidence in lumbar vertebral bodies under cyclic loading. The new implants were evaluated in two different configurations (two small prototypes vs. one large prototype with similar load-bearing area) in comparison to a conventional screw-based implant (MACS TL). A pool of 13 spines with a total of 65 vertebrae was used to establish five testing groups of similar bone mineral density (BMD) distribution with eight lumbar vertebrae each. In additional to BMD assessment via dual-energy X-ray absorptiometry, cancellous BMD and structural parameters were determined using a new generation in vivo 3D-pQCT. The specimens were loaded sinusoidally in force control at 1 Hz for 1000 cycles at three load levels (100, 200, and 400 N). A survival analysis using the number of cycles until failure (Cox regression with covariates) was applied to reveal differences between implant groups. All new prototype configurations except the large cylinder survived significantly longer than the control group. The number of cycles until failure was significantly correlated with the structural parameter Tb.Sp. and similarly with the cancellous BMD for three of five implants. In both large prototypes the cycle number until failure significantly correlated with the preoperative distance to the upper endplates. Although the direct relationship between bone structure or density and mechanical breakage behavior cannot be conclusively proven, all the prototypes adapted for poor bone structure performed better than the comparable conventional implant. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res
- Published
- 2006
24. Spinal neuronal activation during locomotor-like activity enabled by epidural stimulation and 5-hydroxytryptamine agonists in spinal rats
- Author
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Paul O, Duru, Niranjala J K, Tillakaratne, Jung A, Kim, Hui, Zhong, Stacey M, Stauber, Trinh T, Pham, Mei S, Xiao, V Reggie, Edgerton, and Roland R, Roy
- Subjects
Epidural Space ,education ,Electric Stimulation Therapy ,Motor Activity ,Cholinergic Neurons ,Article ,Rats ,Serotonin Receptor Agonists ,Rats, Sprague-Dawley ,Spinal Cord ,Interneurons ,Animals ,Female ,Spinal Cord Injuries - Abstract
The neural networks that generate stepping in complete spinal adult rats remain poorly defined. To address this problem, we used c-fos (an activity-dependent marker) to identify active interneurons and motoneurons in the lumbar spinal cord of adult spinal rats during a 30-min bout of bipedal stepping. Spinal rats were either step trained (30 min/day, 3 days/week, for 7.5 weeks) or not step trained. Stepping was enabled by epidural stimulation and the administration of the serotonergic agonists quipazine and 8-OHDPAT. A third group of spinal rats served as untreated (no stimulation, drugs, or stepping) controls. The numbers of activated cholinergic central canal cluster cells and partition neurons were higher in both step-trained and nontrained rats than in untreated rats and were higher in nontrained than in step-trained rats. The latter finding suggests that daily treatment with epidural stimulation plus serotonergic agonist treatment without step training enhances the excitability of a broader cholinergic interneuronal population than does step training. The numbers of activated interneurons in laminae II-VI of lumbar cross-sections were higher in both step-trained and nontrained rats than in untreated rats, and they were highest in step-trained rats. This finding suggests that this population of interneurons is responsive to epidural stimulation plus serotonergic treatment and that load-bearing induced when stepping has an additive effect. The numbers of activated motoneurons of all size categories were higher in the step-trained group than in the other two groups, reflecting a strong effect of loading on motoneuron recruitment. In general, these results indicate that the spinal networks for locomotion are similar with and without brain input.We identified neurons within the spinal cord networks that are activated during assisted stepping in paraplegic rats. We stimulated the spinal cord and administered a drug to help the rats step. One group was trained to step and another was not trained. We observed a lower percentage of activated neurons in specific spinal cord regions in trained rats than in nontrained rats after a 1-hr stepping bout, suggesting that step training reduces activation of some types of spinal neurons. This observation indicates that training makes the spinal networks more efficient and suggests a "learning" phenomenon in the spinal cord without any brain input.
- Published
- 2014
25. On the lifetimes of evaporating droplets
- Author
-
Khellil Sefiane, Brian R. Duffy, Stephen Wilson, and Jutta M. Stauber
- Subjects
Materials science ,phase change ,DROPS ,Substrate (electronics) ,Contact angle ,Physics::Fluid Dynamics ,Phase change ,Solid substrate ,SUBSTRATE ,Contact radius ,QA273 ,NANOPARTICLES ,HYSTERESIS ,KINETICS ,condensation/evaporation ,Mechanical Engineering ,Condensed Matter Physics ,Hysteresis ,Hydrophobic surfaces ,Mechanics of Materials ,SESSILE DROPLETS ,HYDROPHOBIC SURFACES ,TJ ,Atomic physics ,Constant (mathematics) ,SUPERHYDROPHOBIC SURFACE - Abstract
The complete description of the lifetime of a droplet on a solid substrate evaporating in a ‘stick–slide’ mode is obtained. The unexpectedly subtle relationship between the lifetime of such a droplet and the lifetimes of initially identical droplets evaporating in the extreme modes (namely the constant contact radius and constant contact angle modes) is described and summarised in an appropriate master diagram. In particular, it is shown that the lifetime of a droplet is not, in general, constrained by the lifetimes of the extreme modes.
- Published
- 2014
26. Patterns of Mammography Use among Hispanic, American Indian, and Non-Hispanic White Women in New Mexico, 1994-1997
- Author
-
Frank D. Gilliland, William C. Hunt, Charles R. Key, Patricia M. Stauber, and Robert D. Rosenberg
- Subjects
Adult ,Gerontology ,medicine.medical_specialty ,Epidemiology ,New Mexico ,Population ,Psychological intervention ,Ethnic group ,Breast Neoplasms ,Breast cancer ,Humans ,Mass Screening ,Medicine ,Mammography ,Community Health Services ,education ,Mass screening ,Aged ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Public health ,Hispanic or Latino ,Middle Aged ,medicine.disease ,Indians, North American ,Female ,business ,Demography - Abstract
For screening efforts to maximally reduce mortality in the general population, a large proportion of women need to utilize mammography routinely. To investigate utilization of mammography in a community setting, the authors used population-based data collected by the New Mexico Mammography Project for residents of the Albuquerque, New Mexico, metropolitan area for the period 1994-1997. The authors computed screening rates and the proportion of women who routinely use mammography. The utilization of mammography was low. Only 50% of the women aged 50-74 years were screened each year. Less than one third of women aged 40-49 years or 75 years and older were screened annually. The percentage of women who routinely used mammography on an annual or biennial basis was low in all age groups, especially among Hispanics and American Indians. Women aged 50-74 years had the highest percentage of routine annual mammography use, ranging from 30% in non-Hispanic Whites to 20% in Hispanics. Current utilization of mammography in community-based screening efforts is unlikely to achieve a potential 30% reduction in breast cancer mortality. Interventions are needed to increase the routine use of mammography.
- Published
- 2000
27. Biologic Characteristics of Interval and Screen-Detected Breast Cancers
- Author
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Nancy E. Joste, Frank D. Gilliland, Patricia M. Stauber, William C. Hunt, Robert D. Rosenberg, Gillian Redlich, and Charles R. Key
- Subjects
Adult ,Oncology ,Cancer Research ,medicine.medical_specialty ,Time Factors ,Population ,Apoptosis ,Breast Neoplasms ,Breast cancer ,Internal medicine ,medicine ,Humans ,Mass Screening ,Mammography ,Breast ,Risk factor ,education ,Mass screening ,Aged ,Gynecology ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Cancer ,Odds ratio ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Confidence interval ,Ki-67 Antigen ,Proto-Oncogene Proteins c-bcl-2 ,Data Interpretation, Statistical ,Female ,Tumor Suppressor Protein p53 ,business - Abstract
Background: Interval breast cancer is defined as a cancer that is detected within 12 months after a negative mammogram. The failure of mammography to detect breast cancer depends on testing procedures, radiologist interpretation, patient characteristics, and tumor properties. Although errors by radiologists explain some interval cancers, another explanation is that the tumor is rapidly growing and was too small to be detected on the last mammogram. To determine whether markers of tumor growth rate are associated with risk of an interval cancer, we conducted a population-based study with the use of data collected statewide by the New Mexico Mammography Project. Methods: Among women who received a mammographic examination from 1991 throughout 1993, we ascertained records of all patients with breast cancer diagnosed within 12 months of a negative screening mammographic examination (interval cancers) and corresponding tumor samples, when available. We selected an age-and ethnicity-matched comparison group of control patients with screen-detected breast cancers diagnosed during the same period. In tumor samples, p53, bcl-2, and Ki-67 were examined immunologically and the apoptotic index was assessed histologically. We used logistic regression to determine whether interval cancers were associated with selected demographic, radiologic, and biologic characteristics. Results: It is more likely that mammography did not detect tumors with a high proportion of proliferating cells (>20%) than tumors with a low proportion of proliferating cells (
- Published
- 2000
28. Application of Bioelectrical Impedance Analysis to Elderly Populations
- Author
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Gerard E. Dallal, Marian T. Hannan, Richard N. Baumgartner, Patricia M. Stauber, Peter W.F. Wilson, Tamara B. Harris, Douglas P. Kiel, Ronenn Roubenoff, and Christina D. Economos
- Subjects
Aging ,medicine.medical_specialty ,education.field_of_study ,Framingham Risk Score ,business.industry ,Healthy population ,Population ,Mean age ,Models, Biological ,Surgery ,Absorptiometry, Photon ,Framingham Heart Study ,Evaluation Studies as Topic ,Fat free mass ,Statistics ,Body Composition ,Electric Impedance ,medicine ,Humans ,Population study ,Geriatrics and Gerontology ,business ,education ,Bioelectrical impedance analysis ,Aged - Abstract
Background Bioelectrical impedance analysis (BIA) can potentially be used to estimate body composition in large populations studied at multiple sites. However, it is not clear whether age-specific BIA equations are necessary for accurate application of BIA to research on elderly subjects. Methods We compared a published equation designed to predict fat-free mass (FFM) that had been derived in a young healthy population (mean age 27 y; mean BMI 23.9 kg/m2), with equations that we developed for the elderly by using data from 455 participants in the Framingham Heart Study (78 Y; 27.3 kg/m2), using dual-energy x-ray absorptiometry (DXA) as a reference technique. The BIA equations were then compared in an independent sample of 283 participants in the New Mexico Aging Process Study (76 y, 25.5 kg/m2), who also underwent BIA and DXA. Results When the young-population equation was applied to Framingham, it caused an overestimation of FFM in heavier subjects that was eliminated by use of the age-specific equation. However, when the two equations were tested in the New Mexico population, the published equation gave estimates of FFM that were closer to DXA than the Framingham equations did. Conclusions The accuracy of a BIA equation depends on the body composition of the population of the population and the validation method rather than on age per se. Application of BIA to elderly populations requires uniform validation procedures in the actual study population, rather than reliance on age-specific equations.
- Published
- 1997
29. Comment on 'Increased evaporation kinetics of sessile droplets by using nanoparticles'
- Author
-
Stephen Wilson, Khellil Sefiane, Jutta M. Stauber, and Brian R. Duffy
- Subjects
Chemical engineering ,Kinetics ,Electrochemistry ,Evaporation ,Nanoparticle ,General Materials Science ,Nanotechnology ,Surfaces and Interfaces ,Condensed Matter Physics ,QA ,Spectroscopy - Abstract
Comment on "Increased evaporation kinetics of sessile droplets by using nanoparticles"
- Published
- 2013
30. On the lifetimes of evaporating droplets with related initial and receding contact angles
- Author
-
Khellil Sefiane, Stephen Wilson, Brian R. Duffy, and Jutta M. Stauber
- Subjects
Fluid Flow and Transfer Processes ,Physics ,FLOW ,Mechanical Engineering ,Solid surface ,Flow (psychology) ,Computational Mechanics ,Evaporation ,LINE ,DROPS ,Mechanics ,Condensed Matter Physics ,Physics::Fluid Dynamics ,Contact angle ,SOLID-SURFACES ,SUBSTRATE ,Classical mechanics ,Hydrophobic surfaces ,Mechanics of Materials ,SESSILE DROPLETS ,HYDROPHOBIC SURFACES ,Two-phase flow ,QA - Abstract
A physically credible relationship based on the unbalanced Young force between theinitial and receding contact angles of an evaporating droplet is proposed and used togive a complete description of the lifetime of a droplet evaporating in an idealisedstick-slide mode. In particular, it is shown that the dependence of the lifetime on theinitial contact angle is qualitatively different from that when the relationship betweenthe initial and receding contact angles is not taken into account.
- Published
- 2015
31. Benign breast biopsy diagnosis and subsequent risk of breast cancer
- Author
-
Robert D. Rosenberg, Patricia M. Stauber, Charles R. Key, and Erin L. Ashbeck
- Subjects
Breast biopsy ,Adult ,Risk ,medicine.medical_specialty ,Epidemiology ,Biopsy ,New Mexico ,Breast Neoplasms ,Breast Diseases ,Breast cancer ,Atypia ,medicine ,Mammography ,Humans ,Registries ,Risk factor ,skin and connective tissue diseases ,Aged ,Proportional Hazards Models ,Gynecology ,Aged, 80 and over ,medicine.diagnostic_test ,Obstetrics ,business.industry ,Cancer ,Middle Aged ,medicine.disease ,Fibroadenoma ,Oncology ,Female ,business - Abstract
Background: We examine benign breast biopsy diagnoses as reported by community pathologists in New Mexico and investigate associations with future breast cancer development. Methods: Using data collected between 1992 and 2000 by the New Mexico Mammography Project and cancer data through 2003 from the New Mexico Tumor Registry, we calculated breast cancer rates following 14,602 benign breast biopsies for women ages 30 to 89 years. For comparison, we also calculated the breast cancer rate following 215,283 normal screening mammograms. Hazard ratios (HR) are presented. Results: We identified 480 subsequent breast cancer diagnoses among 14,602 women with benign breast biopsies and 4,402 breast cancer diagnoses among 215,283 women with mammograms assigned a “negative” or “benign finding” assessment. Histologic diagnoses in absence of atypia had an age-adjusted HR of 1.95 [95% confidence interval (95% CI), 1.77-2.15]. Among low-risk histologic diagnoses, the strongest associations with subsequent breast cancer development included adenosis, apocrine metaplasia, calcifications, and ductal hyperplasia. Fibroadenoma, inflammation, and cysts did not exhibit an association with breast cancer development. Women with low-risk diagnoses and breast tissue characterized as fatty or with scattered densities had a HR of 2.09 (95% CI, 1.68-2.60), whereas women with low-risk histologic diagnoses and dense breasts had a HR of 3.36 (95% CI, 2.83-3.99). Conclusions: The observed breast cancer occurrence contributes to evidence of increased risk following benign biopsy. The risk associated with histologic diagnoses in absence of atypia was twice the risk experienced by women with normal mammogram evaluations and may be modified by breast density. (Cancer Epidemiol Biomarkers Prev 2007;16(3):467–72)
- Published
- 2007
32. Impact of medical and demographic factors on long-term quality of life and body image of breast cancer patients
- Author
-
Ralph Kästner, Wolfgang Janni, B. Strobl, Kristin Härtl, Harald Sommer, Brigitte Rack, and M. Stauber
- Subjects
Adult ,medicine.medical_specialty ,medicine.medical_treatment ,Mammary gland ,Breast Neoplasms ,Mastectomy, Segmental ,Breast cancer ,Quality of life ,Internal medicine ,Surveys and Questionnaires ,Tumor stage ,medicine ,Body Image ,Humans ,Mastectomy ,Aged ,Aged, 80 and over ,Adjuvant radiotherapy ,business.industry ,Age Factors ,Hematology ,Fear ,Middle Aged ,medicine.disease ,humanities ,Surgery ,Outcome parameter ,Radiation therapy ,medicine.anatomical_structure ,Oncology ,Patient Satisfaction ,Quality of Life ,Female ,Neoplasm Recurrence, Local ,business - Abstract
The impact of various medical and demographic factors on the quality of life (QoL) of breast cancer patients has been discussed controversially. We investigated the influence of six different factors on long-term QoL and body image of women with primary breast cancer.Two-hundred and seventy-four breast cancer patients were administered the QoL questionnaire following a mean interval of 4.2 years after primary diagnosis. All women had been primarily treated for stage I to III breast cancer without evidence of distant metastases. QoL was evaluated by using the QLQ-C30 questionnaire Version 2.0. Supplementary scales included body image, satisfaction with surgical treatment, cosmetic result and fear of recurrence. We analyzed the impact of tumor stage, surgical treatment, adjuvant radiotherapy, adjuvant cytotoxic therapy, age and length of follow-up period on the examined outcome parameters.At the time of the follow-up examination, patients showed minor impairment of QoL (mean 67.8) and body image (mean 24.8), but more fear of recurrence (mean 60.7). None of the studied factors had a significant impact on overall QoL (P0.05) according to the QLQ-C30 questionnaire. In contrast, with the exception of the factors 'cytotoxic therapy' and 'radiotherapy' all investigated variables influenced at least one of the additional psychological scales (P0.05). The primary surgical treatment modality had the strongest impact and affected all four scales. Patients treated with breast conservation reported a more favorable body image, compared to those treated with mastectomy (17.2 versus 37.5, P0.01), more satisfaction with surgical treatment (4.0 versus 10.7, P = 0.01), rated a better cosmetic result (75.5 versus 57.1, P0.01), but presented more fear of recurrence (63.9 versus 55.3, P = 0.04).Current QoL questionnaires do not sufficiently cover all relevant aspects of QoL, but might be complemented by breast cancer specific aspects such as body image and fear.
- Published
- 2003
33. Associations of fat and muscle masses with bone mineral in elderly men and women
- Author
-
Linda J. Romero, Kathleen M. Koehler, Patricia M. Stauber, P J Garry, and Richard N. Baumgartner
- Subjects
musculoskeletal diseases ,Male ,medicine.medical_specialty ,Aging ,Bone density ,medicine.drug_class ,Medicine (miscellaneous) ,Adipose tissue ,Muscle mass ,Absorptiometry, Photon ,Bone Density ,Internal medicine ,medicine ,Humans ,Aged ,Bone mineral ,Aged, 80 and over ,Nutrition and Dietetics ,business.industry ,musculoskeletal, neural, and ocular physiology ,Muscles ,Estrogen Replacement Therapy ,Body fatness ,Soft tissue ,musculoskeletal system ,humanities ,Endocrinology ,Adipose Tissue ,Estrogen ,Body Composition ,Bone mineral content ,Regression Analysis ,Female ,business - Abstract
Associations of fat and muscle masses with bone mineral status were studied in 301 men and women aged > or = 65 y. Bone mineral and soft tissue composition were estimated by using dual-energy X-ray absorptiometry. Univariate correlations suggested that muscle is associated more closely than fat with bone mineral content (BMC) as well as with bone mineral density (BMD) in men. In women, however, correlations of BMC with muscle were only slightly greater than those with fat and correlations with BMD were consistently greater with fat than with muscle. This suggests that correlations of BMC with muscle are influenced by bone and body size, especially in women. A multiple-regression model was developed that adjusts BMC for bone area, knee height, age, and the independent effects of fat and muscle. In men, muscle remained more closely associated with adjusted BMC than with fat. In women, fat mass was associated significantly with BMC but muscle mass was not. The exception was for women taking estrogen, in whom neither fat nor muscle was associated significantly with adjusted BMC. This study suggests that body fatness may be more important than muscle in maintaining bone mineral in elderly women not taking estrogen.
- Published
- 1996
34. Prematurely condensed human sperm chromosomes after in vitro fertilization (IVF)
- Author
-
H Kentenich, H. Schmiady, M. Stauber, and Karl Sperling
- Subjects
Male ,Genetics ,In vitro fertilisation ,Pronucleus ,medicine.medical_treatment ,Karyotype ,Fertilization in Vitro ,Biology ,Spermatozoa ,Sperm ,Chromosome Banding ,Andrology ,Polar body ,Human fertilization ,Cytoplasmic chromosome ,Karyotyping ,embryonic structures ,Oocytes ,medicine ,Chromosomes, Human ,Humans ,Female ,Ploidy ,Genetics (clinical) - Abstract
During an in vitro fertilization (IVF) program 122 inseminated eggs showing polar body extrusion, but neither formation of pronuclei nor cell cleavage were analysed cytogenetically. Nine of these eggs showed prematurely condensed sperm chromosomes of the G1-phase (G1-PCC) besides the haploid set of maternal metaphase II chromosomes. This phenomenon can be explained by the permanent arrest of the oocytes at metaphase II after sperm penetration and hence the continuing presence of cytoplasmic chromosome condensing factors which lead to the induction of PCC in the sperm nucleus. The overall frequency of this aberrant type of fertilization was calculated to be in the order of 3-4% of all in vitro fertilized eggs.
- Published
- 1986
35. [Results in the treatment of suppurative skin diseases with a nitrofuran derivative (furacin)]
- Author
-
M, STAUBER
- Subjects
Suppuration ,Nitrofurans ,Nitrofurazone ,Communicable Diseases ,Skin Diseases - Published
- 1961
36. [Problem of epidemic fungus diseases of the foot]
- Author
-
M, STAUBER and J, SZPIKOWSKI
- Subjects
Foot Diseases ,Mycoses ,Tinea ,Foot - Published
- 1954
37. [THE APPEARANCE AND COURSE OF THE HERXHEIMER-LUKASIEWICZ REACTION FOLLOWING THE ADMINISTRATION OF VARIOUS ANTIBIOTICS]
- Author
-
M, STAUBER and T, KAJZER
- Subjects
Chloramphenicol ,Fever ,Hypersensitivity ,Streptomycin ,Humans ,Syphilis ,Antibiotics, Antitubercular ,Anti-Bacterial Agents ,Chlortetracycline ,Erythromycin - Published
- 1963
38. [Therapy of psoriasis with acrichine]
- Author
-
M, STAUBER
- Subjects
Quinacrine ,Humans ,Psoriasis - Published
- 1955
39. [Scleroedema Buschke]
- Author
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H, Maldyk and M, Stauber
- Subjects
Male ,Scleroderma, Systemic ,Adolescent ,Scleredema Adultorum ,Humans - Published
- 1966
40. [Allergic manifestations during the treatment of mycoses with griseofulvin]
- Author
-
M, STAUBER
- Subjects
Drug Hypersensitivity ,Mycoses ,Griseofulvin - Published
- 1962
41. Data-Driven Gradient Regularization for Quasi-Newton Optimization in Iterative Grating Interferometry CT Reconstruction.
- Author
-
van Gogh S, Mukherjee S, Rawlik M, Pereira A, Spindler S, Zdora MC, Stauber M, Varga Z, and Stampanoni M
- Subjects
- Phantoms, Imaging, Algorithms, Tomography, X-Ray Computed methods, Image Processing, Computer-Assisted methods
- Abstract
Grating interferometry CT (GI-CT) is a promising technology that could play an important role in future breast cancer imaging. Thanks to its sensitivity to refraction and small-angle scattering, GI-CT could augment the diagnostic content of conventional absorption-based CT. However, reconstructing GI-CT tomographies is a complex task because of ill problem conditioning and high noise amplitudes. It has previously been shown that combining data-driven regularization with iterative reconstruction is promising for tackling challenging inverse problems in medical imaging. In this work, we present an algorithm that allows seamless combination of data-driven regularization with quasi-Newton solvers, which can better deal with ill-conditioned problems compared to gradient descent-based optimization algorithms. Contrary to most available algorithms, our method applies regularization in the gradient domain rather than in the image domain. This comes with a crucial advantage when applied in conjunction with quasi-Newton solvers: the Hessian is approximated solely based on denoised data. We apply the proposed method, which we call GradReg, to both conventional breast CT and GI-CT and show that both significantly benefit from our approach in terms of dose efficiency. Moreover, our results suggest that thanks to its sharper gradients that carry more high spatial-frequency content, GI-CT can benefit more from GradReg compared to conventional breast CT. Crucially, GradReg can be applied to any image reconstruction task which relies on gradient-based updates.
- Published
- 2024
- Full Text
- View/download PDF
42. Towards clinical-dose grating interferometry breast CT with fused intensity-based iterative reconstruction.
- Author
-
van Gogh S, Rawlik M, Pereira A, Spindler S, Mukherjee S, Zdora MC, Stauber M, Alaifari R, Varga Z, and Stampanoni M
- Subjects
- Contrast Media, Interferometry, Microscopy, Phase-Contrast, Tomography, X-Ray Computed, Algorithms
- Abstract
X-ray grating interferometry CT (GI-CT) is an emerging imaging modality which provides three complementary contrasts that could increase the diagnostic content of clinical breast CT: absorption, phase, and dark-field. Yet, reconstructing the three image channels under clinically compatible conditions is challenging because of severe ill-conditioning of the tomographic reconstruction problem. In this work we propose to solve this problem with a novel reconstruction algorithm that assumes a fixed relation between the absorption and the phase-contrast channel to reconstruct a single image by automatically fusing the absorption and phase channels. The results on both simulations and real data show that, enabled by the proposed algorithm, GI-CT outperforms conventional CT at a clinical dose.
- Published
- 2023
- Full Text
- View/download PDF
43. Micro-cleanliness of Hard Tissue Debris After Advanced Irrigation and Comparison Between EndoVac and XP-endo Finisher: A Microcomputed Tomographic Study.
- Author
-
Alsofi L, Al Harbi M, Al-Habib M, Stauber M, and Balto K
- Abstract
Introduction: Conventional irrigation techniques do not remove debris adequately. The remaining tissue debris cause infection inside the root canal and may also affect the seal of the root canal. The study aimed to compare the ability of EndoVac (EV) with XP-endo finisher (XPF) in debris removal using micro-CT analysis., Materials and Methods: We used 12 lower first permanent molar human teeth for this study. The root canals were instrumented using a small TF adaptive system. Then, the volume of debris was calculated. Teeth were divided into two groups, according to advanced irrigation methods, with six teeth per group: EV group and XPF group. The volume of debris was calculated again. The paired-sample t -test was used to compare the volume of the debris before and after the use of advanced irrigation methods with the statistical significance of P < 0.05. The percentage of debris reduction was also calculated., Results: Both EV and XPF showed a significant decrease of debris in the mesial canals ( P < 0.05), whereas EV only showed a significant reduction of debris in the distal canals., Conclusion: Both EV and XPF were able to significantly reduce debris after instrumentation in the mesial canals of lower first mandibular molars., Clinical Significance: The study provides insight into the recent advanced methods used in debris removal and canal disinfection., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Journal of International Society of Preventive and Community Dentistry.)
- Published
- 2021
- Full Text
- View/download PDF
44. Analysis of the Morpho-Geometrical Changes of the Root Canal System Produced by TF Adaptive vs. BioRace: A Micro-Computed Tomography Study.
- Author
-
Alsofi L, Al Harbi M, Stauber M, and Balto K
- Abstract
We aimed to analyze the morpho-geometric changes of the root canal system created by two rotary systems (TF Adaptive and BioRace) using micro-CT technology. Two concepts of rotary file system kinematics, continuous rotation and adaptive kinematics, were used in root canal preparation. Twenty mandibular molars (n = 20) were selected with the following criteria: the teeth have mesial roots with a single and continuous isthmus connecting the mesiobuccal and mesiolingual canals (Vertucci's Type I configuration) and distal roots with independent canals. Teeth were scanned at a resolution of 14 μm. Canals were divided equally into two groups and then enlarged sequentially using the BioRace system and TF Adaptive system according to manufacturer protocol. Co-registered images, before and after preparation, were evaluated for morphometric measurements of canal surface area, volume, structure model index, thickness, straightening, and un-instrumented surface area. Before and after preparation, data were statistically analyzed using a paired sample t -test. After preparation, data were analyzed using an unpaired sample test. The preparation by both systems significantly changed canal surface area, volume, structure model index, and thickness in both systems. There were no significant differences between instrument types with respect to these parameters ( p > 0.05). TF Adaptive was associated with less straightening (8% compared with 17% for BioRace in the mesial canal, p > 0.05). Both instrumentation systems produced canal preparations with adequate geometrical changes. BioRace straightened the mesial canals more than TF Adaptive.
- Published
- 2021
- Full Text
- View/download PDF
45. A multiscale imaging and modelling dataset of the human inner ear.
- Author
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Gerber N, Reyes M, Barazzetti L, Kjer HM, Vera S, Stauber M, Mistrik P, Ceresa M, Mangado N, Wimmer W, Stark T, Paulsen RR, Weber S, Caversaccio M, and Ballester MAG
- Subjects
- Humans, Image Processing, Computer-Assisted, Ear, Inner diagnostic imaging
- Abstract
Understanding the human inner ear anatomy and its internal structures is paramount to advance hearing implant technology. While the emergence of imaging devices allowed researchers to improve understanding of intracochlear structures, the difficulties to collect appropriate data has resulted in studies conducted with few samples. To assist the cochlear research community, a large collection of human temporal bone images is being made available. This data descriptor, therefore, describes a rich set of image volumes acquired using cone beam computed tomography and micro-CT modalities, accompanied by manual delineations of the cochlea and sub-compartments, a statistical shape model encoding its anatomical variability, and data for electrode insertion and electrical simulations. This data makes an important asset for future studies in need of high-resolution data and related statistical data objects of the cochlea used to leverage scientific hypotheses. It is of relevance to anatomists, audiologists, computer scientists in the different domains of image analysis, computer simulations, imaging formation, and for biomedical engineers designing new strategies for cochlear implantations, electrode design, and others.
- Published
- 2017
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46. 1700012B09Rik, a FOXJ1 effector gene active in ciliated tissues of the mouse but not essential for motile ciliogenesis.
- Author
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Stauber M, Boldt K, Wrede C, Weidemann M, Kellner M, Schuster-Gossler K, Kühnel MP, Hegermann J, Ueffing M, and Gossler A
- Subjects
- Alleles, Animals, Basal Bodies metabolism, Female, Genes, Reporter, Homozygote, Male, Mice, Knockout, Multiprotein Complexes metabolism, Phenotype, Protein Transport, Subcellular Fractions metabolism, Cilia metabolism, Forkhead Transcription Factors metabolism, Morphogenesis
- Abstract
In humans and mice, motile cilia occur on the surface of the embryonic ventral node, on respiratory and ependymal epithelia and in reproductive organs where they ensure normal left-right asymmetry of the organism, mucociliary clearance of airways, homeostasis of the cerebrospinal fluid and fertility. The genetic programme for the formation of motile cilia, thus critical for normal development and health, is switched on by the key transcription factor FOXJ1. In previous microarray screens for murine FOXJ1 effectors, we identified candidates for novel factors involved in motile ciliogenesis, including both genes that are well conserved throughout metazoa and beyond, like FOXJ1 itself, and genes without overt homologues outside higher vertebrates. Here we examine one of the novel murine FOXJ1 effectors, the uncharacterised 1700012B09Rik whose homologues appear to be restricted to higher vertebrates. In mouse embryos and adults, 1700012B09Rik is predominantly expressed in motile ciliated tissues in a FOXJ1-dependent manner. 1700012B09RIK protein localises to basal bodies of cilia in cultured cells. Detailed analysis of 1700012B09Rik
lacZ knock-out mice reveals no impaired function of motile cilia or non-motile cilia. In conclusion, this novel FOXJ1 effector is associated mainly with motile cilia but - in contrast to other known FOXJ1 targets - its putative ciliary function is not essential for development or health in the mouse, consistent with a late emergence during evolution of motile ciliogenesis., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2017
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47. Identification of FOXJ1 effectors during ciliogenesis in the foetal respiratory epithelium and embryonic left-right organiser of the mouse.
- Author
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Stauber M, Weidemann M, Dittrich-Breiholz O, Lobschat K, Alten L, Mai M, Beckers A, Kracht M, and Gossler A
- Subjects
- Animals, Cell Differentiation genetics, Cell Line, Down-Regulation genetics, Gene Expression Regulation, Developmental, Gene Ontology, Genetic Association Studies, Genome, Green Fluorescent Proteins metabolism, Lung embryology, Lung metabolism, Mice, Organ Specificity genetics, Reproducibility of Results, Respiratory Mucosa cytology, Subcellular Fractions metabolism, Transcriptome genetics, Cilia metabolism, Fetus metabolism, Forkhead Transcription Factors metabolism, Organizers, Embryonic metabolism, Organogenesis genetics, Respiratory Mucosa embryology
- Abstract
Formation of motile cilia in vertebrate embryos is essential for proper development and tissue function. Key regulators of motile ciliogenesis are the transcription factors FOXJ1 and NOTO, which are conserved throughout vertebrates. Downstream target genes of FOXJ1 have been identified in a variety of species, organs and cultured cell lines; in murine embryonic and foetal tissues, however, FOXJ1 and NOTO effectors have not been comprehensively analysed and our knowledge of the downstream genetic programme driving motile ciliogenesis in the mammalian lung and ventral node is fragmentary. We compared genome-wide expression profiles of undifferentiated E14.5 vs. abundantly ciliated E18.5 micro-dissected airway epithelia as well as Foxj1
+ vs. Foxj1-deficient foetal (E16.5) lungs of the mouse using microarray hybridisation. 326 genes deregulated in both screens are candidates for FOXJ1-dependent, ciliogenesis-associated factors at the endogenous onset of motile ciliogenesis in the lung, including 123 genes that have not been linked to ciliogenesis before; 46% of these novel factors lack known homologues outside mammals. Microarray screening of Noto+ vs. Noto null early headfold embryos (E7.75) identified 59 of the lung candidates as NOTO/FOXJ1-dependent factors in the embryonic left-right organiser that carries a different subtype of motile cilia. For several uncharacterised factors from this small overlap - including 1700012B09Rik, 1700026L06Rik and Fam183b - we provide extended experimental evidence for a ciliary function., (Copyright © 2017 Elsevier Inc. All rights reserved.)- Published
- 2017
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- View/download PDF
48. CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility.
- Author
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Weidemann M, Schuster-Gossler K, Stauber M, Wrede C, Hegermann J, Ott T, Boldt K, Beyer T, Serth K, Kremmer E, Blum M, Ueffing M, and Gossler A
- Subjects
- Animals, Basal Bodies metabolism, Cytoskeletal Proteins genetics, Forkhead Transcription Factors genetics, Male, Mice, Mice, Knockout, Morphogenesis physiology, Spermatozoa cytology, Transcription, Genetic genetics, Xenopus laevis, Axoneme metabolism, Cytoskeletal Proteins metabolism, Flagella metabolism, Forkhead Transcription Factors metabolism, Sperm Motility physiology, Spermatogenesis physiology, Spermatozoa metabolism
- Abstract
Motile cilia move extracellular fluids or mediate cellular motility. Their function is essential for embryonic development, adult tissue homeostasis and reproduction throughout vertebrates. FOXJ1 is a key transcription factor for the formation of motile cilia but its downstream genetic programme is only partially understood. Here, we characterise a novel FOXJ1 target, Cfap157, that is specifically expressed in motile ciliated tissues in mouse and Xenopus in a FOXJ1-dependent manner. CFAP157 protein localises to basal bodies and interacts with tubulin and the centrosomal protein CEP350. Cfap157 knockout mice appear normal but homozygous males are infertile. Spermatozoa display impaired motility and a novel phenotype: Cfap157-deficient sperm exhibit axonemal loops, supernumerary axonemal profiles with ectopic accessory structures, excess cytoplasm and clustered mitochondria in the midpiece regions, and defective axonemes along the flagella. Our study thus demonstrates an essential sperm-specific function for CFAP157 and suggests that this novel FOXJ1 effector is part of a mechanism that acts during spermiogenesis to suppress the formation of supernumerary axonemes and ensures a correct ultrastructure., (© 2016. Published by The Company of Biologists Ltd.)
- Published
- 2016
- Full Text
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49. Micro-CT vs. Whole Body Multirow Detector CT for Analysing Bone Regeneration in an Animal Model.
- Author
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Bissinger O, Kirschke JS, Probst FA, Stauber M, Wolff KD, Haller B, Götz C, Plank C, and Kolk A
- Subjects
- Animals, Bone Density, Bone and Bones anatomy & histology, Disease Models, Animal, Male, Rats, Rats, Sprague-Dawley, Tomography, X-Ray Computed, Bone Regeneration physiology, Bone and Bones diagnostic imaging, X-Ray Microtomography
- Abstract
Objectives: Compared with multirow detector CT (MDCT), specimen (ex vivo) micro-CT (μCT) has a significantly higher (~ 30 x) spatial resolution and is considered the gold standard for assessing bone above the cellular level. However, it is expensive and time-consuming, and when applied in vivo, the radiation dose accumulates considerably. The aim of this study was to examine whether the lower resolution of the widely used MDCT is sufficient to qualitatively and quantitatively evaluate bone regeneration in rats., Methods: Forty critical-size defects (5mm) were placed in the mandibular angle of rats and covered with coated bioactive titanium implants to promote bone healing. Five time points were selected (7, 14, 28, 56 and 112 days). μCT and MDCT were used to evaluate the defect region to determine the bone volume (BV), tissue mineral density (TMD) and bone mineral content (BMC)., Results: MDCT constantly achieved higher BV values than μCT (10.73±7.84 mm3 vs. 6.62±4.96 mm3, p<0.0001) and consistently lower TMD values (547.68±163.83 mm3 vs. 876.18±121.21 mm3, p<0.0001). No relevant difference was obtained for BMC (6.48±5.71 mm3 vs. 6.15±5.21 mm3, p = 0.40). BV and BMC showed very strong correlations between both methods, whereas TMD was only moderately correlated (r = 0.87, r = 0.90, r = 0.68, p < 0.0001)., Conclusions: Due to partial volume effects, MDCT overestimated BV and underestimated TMD but accurately determined BMC, even in small volumes, compared with μCT. Therefore, if bone quantity is a sufficient end point, a considerable number of animals and costs can be saved, and compared with in vivo μCT, the required dose of radiation can be reduced., Competing Interests: Scanco Medical AG provided support in the form of salaries for MS, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of MS is articulated in the ‘author contributions’ section. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2016
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50. Automated 3D-2D registration of X-ray microcomputed tomography with histological sections for dental implants in bone using chamfer matching and simulated annealing.
- Author
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Becker K, Stauber M, Schwarz F, and Beißbarth T
- Subjects
- Algorithms, Humans, Image Enhancement methods, Image Interpretation, Computer-Assisted methods, Orthognathic Surgical Procedures, Pattern Recognition, Automated methods, Radiography, Dental methods, Reproducibility of Results, Sensitivity and Specificity, Dental Implants, Imaging, Three-Dimensional methods, Jaw anatomy & histology, Jaw diagnostic imaging, Subtraction Technique, Tomography, X-Ray Computed methods
- Abstract
We propose a novel 3D-2D registration approach for micro-computed tomography (μCT) and histology (HI), constructed for dental implant biopsies, that finds the position and normal vector of the oblique slice from μCT that corresponds to HI. During image pre-processing, the implants and the bone tissue are segmented using a combination of thresholding, morphological filters and component labeling. After this, chamfer matching is employed to register the implant edges and fine registration of the bone tissues is achieved using simulated annealing. The method was tested on n=10 biopsies, obtained at 20 weeks after non-submerged healing in the canine mandible. The specimens were scanned with μCT 100 and processed for hard tissue sectioning. After registration, we assessed the agreement of bone to implant contact (BIC) using automated and manual measurements. Statistical analysis was conducted to test the agreement of the BIC measurements in the registered samples. Registration was successful for all specimens and agreement of the respective binary images was high (median: 0.90, 1.-3. Qu.: 0.89-0.91). Direct comparison of BIC yielded that automated (median 0.82, 1.-3. Qu.: 0.75-0.85) and manual (median 0.61, 1.-3. Qu.: 0.52-0.67) measures from μCT were significant positively correlated with HI (median 0.65, 1.-3. Qu.: 0.59-0.72) between μCT and HI groups (manual: R(2)=0.87, automated: R(2)=0.75, p<0.001). The results show that this method yields promising results and that μCT may become a valid alternative to assess osseointegration in three dimensions., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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