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Introduction. Factors affecting the severity of the course of a new coronavirus infection remain unexplored to the endAim. To study the factors associated with the transition of COVID-19 from moderate to severe form of the disease in patients undergoing inpatient treatmentMaterials and methods. The diagnosis of COVID-19-associated pneumonia was made by a clinician on the basis of PCR test data and an up-to-date chest CT scan. A total of 195 consecutively recruited patients were examined. The average age of the surveyed was 54 (12.1) years.Results. Significantly more patients with severe coronavirus infection at the time of hospitalization were active tobacco smokers (33 (29.73%) vs 67 (79.6%); p < 0.002) and had a BMI consistent with pre-obesity/obesity (42 (37.84%) vs 44 (52.38%); p = 0.027). In patients with severe COVID-19, a significantly higher content of leukocytes was noted in the complete blood count (6.054 (2.813) vs 7.307 (4.707); p = 0.021). The groups also differed significantly in the content of micro- and macroelements. The imbalance of minerals revealed in our study is published for the first time, because. The study of the content of Al, B, Li, Co, Sr and Si in patients with COVID-19 was not carried out.Conclusion. The revealed changes in the microelement status require further study to confirm their significance in the development of a severe form of a new coronavirus infection in patients.

Relevance. The issues of reproduction of fertility of light gray forest soils of the forest-steppe zone and utilization of poultry industry waste as organic fertilizers are interrelated and are very relevant. Methods. The experiments studied the effect of one-time application of various doses (15, 30, 50 t/ha) of chicken manure, chicken manure compost and local organic fertilizers (sapropel and peat) on crop yields, and the aftereffect of fertilizers on the main indicators of soil fertility — amount of humus, nitrate nitrogen, phosphorus and potassium. The variants of the experiments were investigated sixfold. The Federal State Center of Agrochemical Service "Chuvashsky" was involved in laboratory analyses of soils and crop production. Mеthods of analysis of soil samples and crop production are generally accepted in agrochemical science.Results. Organic fertilizers for three years of research were introduced into the soil only once at the beginning of the experiment in doses of 15, 30 and 50 t/ha, and their effect on the yield and quality of crop production (potato tubers, spring wheat grains and peas) in 2020–2022 was positive. The agrochemical properties of the arable soil layer in the variants with the use of organic fertilizers have significantly improved. The nitrate content in the products in variants with doses of chicken manure and compost of 50 t/ha or more approached the critical level. Local fertilizers (sapropel and peat) at a dose of 30 t/ha, chicken manure and compost at a dose of 15 t/ha are good organic fertilizers with a long aftereffect.

Aim. Define the safety and effectiveness of rendezvous technique for choledocholithiasis.Material and methods. We performed a retrospective and prospective analysis of the results in percutaneous transhepatic access to the bile ducts and endoscopic intervention in the rendezvous technique for choledocholithiasis in 100 elderly patients. The patients were divided into 2 groups for 50 people. The implementation of the rendezvous began from the antegrade stage in patients from group I; in patients of group II – from the retrograde stage. Postmanipulation complications and failures were When analyzed. The control point of the study is the identification of complications associated with manipulation from the next stages of the rendezvous technique.Results. We revealed a direct dependence of the results in sequence of rendezvous technique for elderly patients with choledocholithiasis and obstructive jaundice. This fact allows recommending percutaneous transhepatic access before endoscopic intervention for these patients with statistical significance. The frequency of complications and failures in patients of group I was 18%, in group II – 52%. This allows for such patients percutaneous transhepatic access before endoscopic intervention in the rendezvous technique. The frequency of complications and failures in patients of group I was 18%, in group II – 52%.Conclusion. Dilatation of intrahepatic segmental and subsegmental 2 and 3 biliary ducts with moderate and severe obstructive jaundice is a predictor of complications with ineffective endoscopic approach. In such situations, preventive antegrade drainage of the biliary tract with rendezvous technique makes possible to prevent cholangitis, effectively eliminate biliary hypertension, clarify the severity of stenosis of the papilla with antegrade balloon revision, and also secure delayed endoscopic resolution of biliary pathology.

Purpose: Analysis of infectious complications incidence in different types of percutaneous externalinternal biliary drainage in patients with obstructive jaundice of tumor genesis.Material and methods: The results of using antegrade external-internal drainage of the biliary tree in transpapillary and suprapapillary variants in 110 patients were analyzed. External-internal biliary drainage was performed in stages, after percutaneous transhepatic cholangiostomy or involuntarily primary with proximal obstruction of the biliary tree with bile duct segregation if it is impossible to form a fixing element of drainage proximal to the obstruction zone.Results: In the first group, transpapillary external-internal drainage was performed in 30 patients with peripapillary tumor obstruction. Of the 26 patients with proximal obstruction, suprapapillary external-internal drainage was performed in 8 patients, transpapillary — in 18 patients. Postmanipulation cholangitis in the first group occurred in 16 cases (28.6 %), liver abscesses developed 4 cases (7.1 %). In the second group, among 30 patients with transpapillary drainage on the background of peripapillary tumor obstruction, signs of acute cholangitis developed in 4 cases. Cholangitis was stopped by timely transfer of external-internal drainage to external. Among 24 patients with proximal obstruction of the biliary tree, suprapapillary external-internal drainage without complications was performed in 18 cases, transpapillary in 6 patients with the proximal block without disconnecting of the biliary tree. Acute cholangitis developed in 2 cases. Patients of the second group had no liver cholangigenic abscesses. There were no cases of hospital mortality in both groups.Conclusion: Factors in the development of postmanipulation cholangitis and liver abscesses during external-internal drainage of the biliary tree against the background of its tumor obstruction are the transpapillary position of endobiliary drainage with duodeno-biliary reflux in persistent biliary hypertension. In the case of suprapapillary location of the working end of external-internal drainage during antegrade drainage of the proximal tumor obstruction of the biliary tree with dissociation, the risk of postmanipulation cholangitis in non-drained liver segments is minimal. In the event of post-manipulation cholangitis in the case of transpapillary drainage of the biliary tree, a temporary transformation of external-internal drainage into external cholangiostomy is necessary.

The results of the study on the effect of new microbial preparations on the dynamics of the absolute weight and average daily gain of geese are presented. In the scientific experiment, probiotics Vetom 20.76 based on the predatory fungus Arthrobotrys oligospora and Vetom 1 on the basis of live spore-forming bacteria of the Bacillus subtilis DSM 32424 strain, which have anthelmintic, antiviral and antifungal effects, were used. One control and four experimental groups of 10 goslings each at the age of 1 month were formed according to the principle of analog pairs. The goslings of the experimental groups received Vetom 20.76 in various dosages: young birds of the 1st experimental group – 0.5 μl/kg of live body weight, the 2nd – 1 μl/kg, the 3rd – 2 μl/kg. Goslings of the 4th experimental group were given Vetom 1 at a dose of 50 mg/kg of live body weight. Both drugs were given in the morning with water, once a day for 30 days. These drugs were not prescribed to geese of the control group. It was established that Preparations Vetom 20.76 in doses of 0.5; 1 and 2 μl/ kg of body weight and Vetom 1 at a dose of 50 mg/kg of body weight have a growth-stimulating effect when given to goslings for 30 days. The growth rate of the experimental birds depended on the dose of the drugs used. Optimal results were obtained with the use of Vetom 20.76 at a dose of 2 μl/ kg of body weight and Vetom 1 at a dose of 50 mg/kg of body weight once a day for 30 days. The average daily gain in live weight of the experimental geese increased in the 3d and 4th experimental groups by 5.24 and 20.60% in the first 15 days of the experiment and by 24.8 and 44.64% during the aftereffect of the drug.

The effect of the partial substitution of M = Ti, V, Cr, Zr, and Nb for Fe in the Pr2Fe17 and Tm2Fe17 alloys on their structural, magnetic, and magnetocaloric properties have been studied using magnetic measurements, X-ray, and neutron diffraction. The character of substitution of Ti, V, Cr, Zr, and Nb atoms for Fe atoms, along with the differences in atomic radii of these elements, substantially affects variations of the lattice parameters and Curie temperatures of the Tm2Fe17 – xMx and Pr2Fe17 – xMx alloys. The Curie temperature of all the substituted alloys increases; the rate of the increase in the Curie temperature of the Tm2Fe17 – xMx alloys is twice that of the Pr2Fe17 – xMx alloys. The magnetic moment of iron atoms MFe and the magnetocaloric effect –ΔSM decrease for the substituted Tm2Fe17 – xMx and Pr2Fe17 – xMx alloys and become close in the case of all M.

Lipofuscin granules (LGs) are accumulated in the retinal pigment epithelium (RPE) cells. The progressive LG accumulation can somehow lead to pathology and accelerate the aging process. The review examines composition, spectral properties and photoactivity of LGs isolated from the human cadaver eyes. By use of atomic force microscopy and near-field microscopy, we have revealed the fluorescent heterogeneity of LGs. We have discovered the generation of reactive oxygen species by LGs, and found that LGs and melanolipofuscin granules are capable of photoinduced oxidation of lipids. It was shown that A2E, as the main fluorophore (bisretinoid) of LGs, is much less active as an oxidation photosensitizer than other fluorophores (bisretinoids) of LGs. Photooxidized products of bisretinoids pose a much greater danger to the cell than non-oxidized one. Our studies of the fluorescent properties of LGs and their fluorophores (bisretinoids) showed for the first time that their spectral characteristics change (shift to the short-wavelength region) in pathology and after exposure to ionizing radiation. By recording the fluorescence spectra and fluorescence decay kinetics of oxidized products of LG fluorophores, it is possible to improve the methods of early diagnosis of degenerative diseases. Lipofuscin ("aging pigment") is not an inert "slag". The photoactivity of LGs can pose a significant danger to the RPE cells. Fluorescence characteristics of LGs are a tool to detect early stages of degeneration in the retina and RPE.

The primary stages of the Exiguobacterium sibiricum rhodopsin (ESR) photocycle were investigated by femtosecond absorption laser spectroscopy in the spectral range of 400-900 nm with a time resolution of 25 fs. The dynamics of the ESR photoreaction were compared with the reactions of bacteriorhodopsin (bR) in purple membranes (bRPM) and in recombinant form (bRrec). The primary intermediates of the ESR photocycle were similar to intermediates I, J, and K in bacteriorhodopsin photoconversion. The CONTIN program was applied to analyze the characteristic times of the observed processes and to clarify the reaction scheme. A similar photoreaction pattern was observed for all studied retinal proteins, including two consecutive dynamic Stokes shift phases lasting ∼0.05 and ∼0.15 ps. The excited state decays through a femtosecond reactive pathway, leading to retinal isomerization and formation of product J, and a picosecond nonreactive pathway that leads only to the initial state. Retinal photoisomerization in ESR takes 0.69 ps, compared with 0.48 ps in bRPM and 0.74 ps in bRrec. The nonreactive excited state decay takes 5 ps in ESR and ∼3 ps in bR. We discuss the similarity of the primary reactions of ESR and other retinal proteins.

Lipofuscin granules accumulate in the retinal pigment epithelium (RPE) with age, especially in patients with visual diseases, including progressive age-related macular degeneration (AMD). Bisretinoids and their photooxidation and photodegradation products are major sources of lipofuscin granule fluorescence. The present study focused on examining the fluorescence decay characteristics of bisretinoid photooxidation and photodegradation products to evaluate the connection between fluorescence lifetime and spectral characteristics of target fluorophore groups. The primary objective of the study was to apply experimental spectral analysis results of lipofuscin granule fluorescence properties to interpretation of fluorescence lifetime imaging ophthalmoscopy data. Fluorescence analysis of the lipofuscin granule fluorophores in RPE collected from cadaver eyes was performed. The fluorescence lifetimes were measured by picosecond-resolved time correlated single photon counting technique. A global analytical method was applied to analyze data sets. The photooxidation and photodegradation products of bisretinoids exhibited a longer fluorescence lifetime (average value approximately 6 ns) and a shorter wavelength maximum (530–580 nm). Further, these products significantly contributed (more than 30%), to total fluorescence compared to the other fluorophores in lipofuscin granules. Thus, the contribution of oxidized lipofuscin bisretinoids to autofluorescence decay kinetics is an important characteristic for fluorescence lifetime imaging microscopy data analysis. The higher average fluorescence lifetime in AMD eyes was likely due to the higher abundance of oxidized bisretinoids compared with non-oxidized bisretinoids. Because higher level of oxidized bisretinoids is indicative of pathological processes in the retina and RPE, the present findings have the potential to improve fluorescence lifetime imaging approaches for early diagnosis of degenerative processes in the retina and RPE.

The article provides an overview of the frequency of distribution of pyometra in cats. Risk factors, clinical manifestations and clinical forms of the disease are analyzed. Current methods of pyometra diagnostics and clinical and laboratory criteria for early detection of signs of sepsis (systemic inflammatory response) in cats with pyometra are considered. The methods of pyometra therapy are analyzed. It was emphasized that the most effective and safest method of treating any form of pyometra is a total ovariohisterectomy in combination with broad-spectrum antibiotics. Encouraging results in the treatment of an open uncomplicated form of pyometers in cats were obtained using, along with antibiotics, preparations based on PgF2alfa and aglepristone.

Aim.To determine the role of antegrade X-ray surgical interventions in the treatment of benign postoperative biliary strictures.Material and methods.A retrospective analysis of treatment of 36 patients with benign biliary strictures was performed. Isolated stricture of biliodigestive anastomosis was diagnosed in 25 cases, partial clipping of common hepatic duct proximal to biliodigestive anastomosis – in 3 cases, partial clipping of bile duct – in 3 patients, isolated biliary strictures – in 5 patients including 4 of them with stricture within previously deployed T-shaped drainage. At the first stage, percutaneous transhepatic cholangiostomy was performed. According to antegrade cholangiography data, structure type “+1, +2” by H. Bismuth classification in modification of E. I. Galperin was diagnosed in 16 (55.2%) patients, proximal biliary strictures (“0”–“−2”) in 13 patients. Three patients with partial clipping of common bile duct and 4 patients with benign biliary stricture in the area of previously deployed T-shaped drainage were not classified. Recanalization of strictures by “catheter-guide” system was followed by antegrade dilatation of the stricture. Final stage was frame external-internal drainage for 6–12 months with stepwise redo balloon dilatation every 3 months (35 patients). The criterion for the end of minimally invasive treatment was the absence of balloon waist in the stricture zone observed during the next procedure but not earlier than in 6 months from primary balloon dilatation. Surgical correction was indicated for recurrent stricture.Results.Direct technical success was achieved in 35 patients. There was 1 case of recurrent strictures within 1 year among 3 cases of primary repair of biliary strictures in the area of previously installed T-shaped drainage. Resection of common bile duct stricture was followed by Roux-en-Y hepaticojejunostomy. Recurrence-free period among 36 patients ranged from 1 to 10 years, median – 56 months. There were no mortality and complications after X-ray surgery.Conclusion.Antegrade recanalization and balloon dilatation of the stricture followed by long-term external-internal biliodigestive frame drainage are effective for both stricture of biliodigestive anastomosis and partial clipping of bile duct. Conventional surgical procedures should be preferred for cicatricial strictures of extrahepatic bile ducts after previous T-shaped drainage deployment.

We have studied dark-adaptation at three levels in the eyes of the crustacean Mysis relicta over 2–3 weeks after exposing initially dark-adapted animals to strong white light: regeneration of 11-cis retinal through the retinoid cycle (by HPLC), restoration of native rhodopsin in photoreceptor membranes (by MSP), and recovery of eye photosensitivity (by ERG). We compare two model populations (“Sea”, Sp, and “Lake”, Lp) inhabiting, respectively, a low light and an extremely dark environment. 11-cis retinal reached 60–70% of the pre-exposure levels after 2 weeks in darkness in both populations. The only significant Lp/Sp difference in the retinoid cycle was that Lp had much higher levels of retinol, both basal and light-released. In Sp, rhodopsin restoration and eye photoresponse recovery parallelled 11-cis retinal regeneration. In Lp, however, even after 3 weeks only ca. 25% of the rhabdoms studied had incorporated new rhodopsin, and eye photosensitivity showed only incipient recovery from severe depression. The absorbance spectra of the majority of the Lp rhabdoms stayed constant around 490–500 nm, consistent with metarhodopsin II dominance. We conclude that sensitivity recovery of Sp eyes was rate-limited by the regeneration of 11-cis retinal, whilst that of Lp eyes was limited by inertia in photoreceptor membrane turnover.

The beginning of 2020 was characterized by the development of a new coronavirus pandemic (COVID-19). Information about the epidemiology, etiology, pathogenesis, clinical and laboratory diagnostics, as well as prevention and therapy for this disease is constantly being expanded and reviewed. The COVID-19 pandemic creates the need for the emergence of new conditions of specialized care for patients with heart rhythm and conduction disorders [1]. These recommendations are intended for general practitioners, internists, cardiologists, electrophysiologists/arrhythmologists, cardiovascular surgeons, functional diagnostics doctors, anesthesiologists-resuscitators, laboratory diagnostics specialists, health care organizers in the system of organizations and healthcare institutions that provide specialized care to patients with heart rhythm and conduction disorders.

In increasing soil fertility, an important role belongs to legumes, which are capable of fixing nitrogen from the atmosphere. One of these crops is of great interest to soybeans. This crop can be successfully cultivated both green manure and fodder - for grain and green mass. For this, we studied four links of the crop rotation. The research was carried out on the experimental field of the Department of Agriculture, Plant Growing, Breeding and Seed Production of the Chuvash State Agrarian University in 2018-2020. Experimental data showed that the most effective in all quantitative indicators are the links of crop rotation with leguminous crops - soybeans. The most productive were the links in the crop rotation: the second link in the crop rotation: cultivated soybeans - potatoes - barley and the fourth link in the crop rotation: cultivated soybeans - spring wheat - barley. The yield of potatoes and spring wheat in the second year after the soybean predecessor increased by 65.6% and 27.6%, compared with the control. The increase in barley in the aftereffect (third year) was 28.0% and 42.1%, respectively.

The article deals with the manifestation of heterosis by economically valuable traits in monoecious hybrids of hashishfree monoecious hemp. Heterosis is one of the reserves for increasing yields and improving the quality of hemp products. In terms of seed harvest, the vast majority of combinations exceed parental varieties. The highest outbreak of heterosis was obtained by crossing varieties Glukhovskaya-10 and YSO-1. In relation to the most productive parent, the degree of heterosis reaches 38.5%. An increase in seed harvest is a derivative of the heterosis of several components: the weight of the plant, the length of the inflorescence, the weight of seeds per plant, the weight of 1000 seeds and the number of seeds per plant. Of these, the most important are the weight of seeds per plant and the weight of 1000 seeds. According to the harvest of straw and fiber, the manifestation of heterosis is limited. Most hybrids have indicators equal to those of the best parental varieties. However, these hybrids significantly exceed the best monoecious variety YSO-1 recommended for cultivation, which is of practical interest. Hybrid plants are more resistant to adverse conditions than the parent varieties. Homeostasis of hybrids depends on the degree of heterozygosity of the organism, as well as the nature of the interaction of alleles of heterozygous loci. The results of our research show that it is possible to consciously control the process of obtaining highly productive hybrid seeds, spending a small amount of labor and funds for its implementation.

Sowing spring triticale based on mixtures of varieties may be more promising than sowing monoculture varieties. Four varieties of spring triticale (Ulyana, Rovnya, Saur and Khaikar) were evaluated with two-component (1:1) and three-component (1:1:1) variety mixtures. Studied 6 variants of two-component mixtures and 4 three-component mixtures. The research was carried out on the experimental field of the Chuvash State University in 2018-2020. The effectiveness of the variety mixtures was determined by a comparative analysis of the actual and possible yield. The stability of crops in terms of yield was assessed by the coefficient of variation. In monoculture, the Saur variety had the highest yield. The two-component crops were the highest yielding and stable over the years compared to the one-component and three-component crops. The most promising variety mixtures turned out to be Ulyana/Khaikar, Rovnya/Saur and Saur/Khaikar, which allow increasing yields by 4.7-20.2 100kg/ha.

The study covered bioelemental state of firemen living and working in North-West region of Russian Federation. The study was aimed to evaluate bioelemental state of firemen in dependence on residence area and on their work features. Regional features of firemen's bioelemental state were revealed. Findings are deficiency of some vitally important bioelements (iodine, selenium, cobalt) and accumulation of toxic microelements (nickel, silver, cadmium, arsenic, aluminium, lead). The authors demonstrated differences in contents of toxic microelements in dependence on length of service and degree of participation in fire-fighting.

PURPOSE: The aim of this work is the determination of quantitative diagnostic criteria based on the spectral characteristics of fundus autofluorescence to detect early stages of degeneration in the retina and retinal pigment epithelium (RPE). METHODS: RPE cell suspension samples were obtained from the cadaver eyes with and without signs of age-related macular degeneration (AMD). Fluorescence analysis at an excitation wavelength of 488 nm was performed. The fluorescence lifetimes of lipofuscin-granule fluorophores were measured by counting time-correlated photon method. RESULTS: Comparative analysis of fluorescence spectra of RPE cell suspensions from the cadaver eyes with and without signs of AMD showed a significant difference in fluorescence intensity at 530–580 nm in response to fluorescence excitation at 488 nm. It was notably higher in eyes with visual pathology than in normal eyes regardless of the age of the eye donor. Measurements of fluorescence lifetimes of lipofuscin fluorophores showed that the contribution of photooxidation and photodegradation products of bisretinoids to the total fluorescence at 530–580 nm of RPE cell suspensions was greater in eyes with visual pathology than in normal eyes. CONCLUSION: Because photooxidation and photodegradation products of bisretinoids are markers of photodestructive processes, which can cause RPE cell death and initiate degenerative processes in the retina, quantitative determination of increases in these bisretinoid products in lipofuscin granules may be used to establish quantitative diagnostic criteria for degenerative processes in the retina and RPE.

Hydroxyapatite was synthesized in conditions of mechanoacoustic treatment of an aqueous reaction mixture consisting of ammonium hydrogen phosphate and calcium nitrate using a commercially available rotary pulsation apparatus. Mechanoacoustic treatment of the reaction mixture was found to lead to the formation of nanosized hydroxyapatite particles with a predominant mean diameter of about 20 nm. Purification of technical hydroxyapatite to remove traces of ammonium nitrate by thermal processing at 350°C is proposed. The crystalline phase of hydroxyapatite was found not to be dominant in the structure. The porosity of hydroxyapatite amounted to about 75% with a mean pore diameter of 2.8 × 103 nm. Thermal processing of hydroxyapatite in aqueous medium was found to yield a paste-like form. Hydroxyapatite paste had no cytotoxicity and did not prevent cell adhesion, though it almost completely inhibited cell spreading.

Aim. To improvethe efficiency of X-ray treatment of postoperative complications (duodenal and biliary fistulae, intraabdominal iatrogenic bile collections). Material and Methods. Percutaneous transhepatic biliary drainage was carried out in 11 patients with nondilated intrahepatic bile ducts. Failure of duodenal stump after previous stomach resections in 5 patients, presence of external biliary fistulae after surgery on gall bladder and common biliary duct in 3 patients and severe pancreonecrosis in 3 cases led to need of antegrade transhepatic cholangiostomy. Results. Antegrade transhepatic cholangiostomy gives an ability to close duodenal fistulae in 5 patients and biliary fistulae in 3 patients with iatrogenic injury of common biliary duct. Percutaneous transhepatic biliary drainage was used in treatment of 3 patients with severe pancreonecrosis. There were 2 complications of antegrade transhepatic cholangiostomy such as hepatic subcapsular hematoma and porto-biliary fistula which were cured effectively using minimally invasive methods (percutaneous drainage of hematoma and disintegration of porto-biliary fistula by filling of intrahepatic canal). Conclusion. Antegradetranshepaticcholangiostomy in case of nondilated intrahepatic bile ducts differs from traditional technique. Often it assumes contrasting of biliary tree using any available way and demands strict compliance of manipulation technology