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5 results on '"Lu, Hai‐Hua"'

1. Synthesis of (+)-7,20-Diisocyanoadociane and Liver-Stage Antiplasmodial Activity of the Isocyanoterpene Class

Author

Lu, Hai-Hua, Pronin, Sergey V, Antonova-Koch, Yevgeniya, Meister, Stephan, Winzeler, Elizabeth A, and Shenvi, Ryan A

Subjects
Medicinal and Biomolecular Chemistry, Chemical Sciences, Liver Disease, Digestive Diseases, Oral and gastrointestinal, Good Health and Well Being, Antimalarials, Heme, Liver, Molecular Structure, Nitriles, Plasmodium falciparum, Pyrenes, Stereoisomerism, General Chemistry, Chemical sciences, Engineering
Abstract

7,20-Diisocyanoadociane, a scarce marine metabolite with potent antimalarial activity, was synthesized as a single enantiomer in 13 steps from simple building blocks (17 linear steps). Chemical synthesis enabled identification of isocyanoterpene antiplasmodial activity against liver-stage parasites, which suggested that inhibition of heme detoxification does not exclusively underlie the mechanism of action of this class.

Published
2016

2. Paleo-soraphens: chemical total syntheses and biological studies

Author

Lu, Hai-Hua, Hinkelmann, Bettina, Tautz, Thomas, Li, Jun, Sasse, Florenz, Franke, Raimo, Kalesse, Markus, Lu, Hai-Hua, Hinkelmann, Bettina, Tautz, Thomas, Li, Jun, Sasse, Florenz, Franke, Raimo, and Kalesse, Markus

Abstract

The soraphens are natural products that exhibit a molecular structure different from what would have been expected by following its polyketidal assembly line. The most significant differences are the presence of a hemiketal instead of a trisubstituted double bond and a double bond at C9 and C10 where a saturated carbon chain was expected. We were interested in the biological activity of the soraphens with architectures as described by the polyketide synthase since we hypothesized that these modifications reflect the evolutionary optimization of the soraphens. Herein we describe four additional derivatives of the so-called paleo-soraphens and their biological profiling to provide a picture of the hypothetical evolutionary optimization of this family of natural products. The syntheses required a unified and convergent strategy and their biological profiling was performed with the aid of impedance measurements. The results of these biological experiments are consistent with the proposed evolutionary optimization of the soraphens.

Published
2015

3. The vinylogous Mukaiyama aldol reaction (VMAR) in natural product synthesis

Author

Kalesse, Markus, Cordes, Martin, Symkenberg, Gerrit, Lu, Hai-Hua, Kalesse, Markus, Cordes, Martin, Symkenberg, Gerrit, and Lu, Hai-Hua

Abstract

The vinylogous Mukaiyama aldol reaction (VMAR) allows efficient access to larger segments for complex natural product synthesis, primarily polyketides, through the construction of vicinal hydroxyl and methyl groups as well as di and tri-substituted double bonds in one single operation. In this review, we will highlight stereoselective protocols that have been used in natural product synthesis and cluster them into the four groups that can be obtained from different silyl ketene acetals or enol ethers. At the beginning, an overview on different stereoselective VMARs is presented; disregarding their applications in total syntheses.

Published
2014

5. [Committed differentiation of transplanted bone derived mesenchymal stem cells and their potential to amend damaged liver functions: in vivo experiment with mice].

Author

Lu HH, Teng GJ, Ju SH, Sun JH, Li AM, and Zhang AF

Subjects
Animals, Carbon Tetrachloride, Cell Transdifferentiation, Cells, Cultured, Chemical and Drug Induced Liver Injury, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Liver Diseases physiopathology, Liver Function Tests, Mesenchymal Stem Cells metabolism, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Nude, Mice, Transgenic, Microscopy, Confocal, Microscopy, Fluorescence, Random Allocation, Cell Differentiation, Liver Diseases surgery, Mesenchymal Stem Cell Transplantation methods, Mesenchymal Stem Cells cytology
Abstract

Objective: To observe whether bone mesenchymal stem cells (MSCs) have the potential to transdifferentiate into functional hepatocyte-like cells in the special "niche" as well as the therapeutic feasibility to repair damaged liver in mice., Methods: 20 nude mice were randomly divided into four groups (n = 5 in each group): Group A: 1.0 ml/kg of carbon tetrachloride (CCl(4)) (dissolved in olive oil by ratio of 1:1) was injected into the peritoneum of mice twice a week for 5 weeks. GFP-positive MSCs (1 x 10(6) cells) were injected into the caudal tail vein 1 week after the first dose of CCl(4); Group B: treated with CCl(4) as in A, but received the same volume of saline; Group C: normal nude mice with GFP-positive MSCs Transplanted in the same way as in A. Group D: normal controls. 4 weeks after the cell transplantation, all animal subjects were killed. Liver function tests (LFT), histology of HE and Masson staining as well as double immunofluorescent staining for GFP and albumin were studied in all groups., Results: The hepatic fibrosis in group A & B confirmed the success of model for liver damage and there was no marked difference in the percent of the area occupied by collagen between two groups (10.5 +/- 1.5 vs 12.7 +/- 1.6, t = -2.238, P > 0.05). GFP-positive MSCs were mainly observed around portal area or interspace of lobules in group A. Some of GFP-positive cells also express albumin (35% +/- 7%). While in group B, C or D, there is no such findings. The level of serum albumin in group A was higher than that in group B (24.4 g/L +/- 3.3 g/L vs 18.6 g/L +/- 2.9 g/L, P < 0.05) while the level of ALT was also different between two groups (121 U/L +/- 21 U/L vs 192 U/L +/- 29 U/L, P < 0.05)., Conclusion: The stimulus of persistent liver damage might enhances the migration of MSCs to the liver, in which some of the MSCs have the potential to transdifferentiate into hepatocyte-like cells. Transplantation of MSCs might amend the damaged tissue of host liver to a certain extent.

Published
2007

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