29 results on '"Lo Bianco, C."'
Search Results
2. Tacrolimus ointment in patients with allergic contact dermatitis to nickel sulphate
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Pacor, Maria Luisa, Mansueto, P., Martinelli, Nicola, Esposito Pellitteri, M., Lo Bianco, C., Leto Barone, M., Ditta, V., D'Alcamo, A., Corrocher, Roberto, Rini, G., Di Lorenzo, G., PACOR M, MANSUETO P, MARTINELLI N, ESPOSITO-PELLITTERI M, LO BIANCO C, LETO-BARONE M, DITTA V, D'ALCAMO A, CORROCHER R, RINI G, and DI LORENZO G
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- 2007
3. Human leucocyte antigen class I and class II and chronic idiopathic urticaria associated with aspirin and/or NSAIDs hypersensitivity
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Pacor, Maria Luisa, Mansueto, P., Martinelli, Nicola, Esposito Pellitteri, M., Ditta, V., Lo Bianco, C., Leto Barone, M., Corrocher, Roberto, Rini, G., D'Alcamoa, A., Di Lorenzo, G., Pacor, M., Mansueto, P., Martinelli, N., ESPOSITO PELLITTERI, M., Ditta, V., LO BIANCO, C., LETO BARONE MS, Corrocher, R., Rini, G., D'Alcamo, A., and DI LORENZO, G.
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- 2007
4. Food additive-induced urticaria: a survey of 838 patients with recurrent chronic idiopathic urticaria
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DI LORENZO, G., Pacor, Maria Luisa, Mansueto, P., Martinelli, Nicola, ESPOSITO PELLITTERI, M., LO BIANCO, C., Ditta, V., LETO BARONE, M. S., Napoli, N., DI FEDE, G., Rini, G. B., and Corrocher, Roberto
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- 2005
5. Strategie terapeutiche mediche della poliposi nasale
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Di Lorenzo, G., Pacor, Maria Luisa, Mansueto, P., Esposito Pellitteri, M., Ditta, V., Lo Bianco, C., MS Leto Barone, Martinelli, Nicola, Di Fede, G., and Rini, Gb
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- 2005
6. Erratum: Randomized placebo-controled trial comparing fluticasone aqueous nasal spray in mono-therapy, fluticasone plus cetirizine, fluticasone plus montelukast and cetirizine plus montelukast for seasonal allergic rhinitis (Clinical and Experimental Allergy (2004) 34 (259-67))
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Di Lorenzo, G., Pacor, M. L., Pellitteri, M. E., Morici, G., Di Gregoli, A., Lo Bianco, C., Ditta, V., Martinelli, N., Candore, G., Mansueto, P., Rini, G. B., Corrocher, R., and Caruso, C.
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montelukast ,seasonal allergic rhinitis ,fluticasone aqueous nasal spray ,cetirizine - Published
- 2004
7. MEASUREMENT OF INFLAMMATORY MEDIATORS OF EOSINOPHILS AND LYMPHOCYTESIN BLOOD IN ACUTE ASTHMA: SERUM LEVELS OF ECP MAY SERVE A PREDICTOR OF BRONCHODILATOR RESPONSE
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DI LORENZO, G., Pacor, Maria Luisa, Drago, A., ESPOSITO PELLITERI, M., Candore, G., LO BIANCO, C., and Caruso, C.
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- 2002
8. Measurement of inflammatory mediators of eosinophils and lymphocytes in blood in acute asthma: serum levels of ECP may serve a predictor of bronchodilator response
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Di Lorenzo, G., Pacor, Maria Luisa, Drago, A., Esposito Pelliteri, M., Candore, G., Lo Bianco, C., and Caruso, C.
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- 2002
9. Nonlinear Variable Structure Filter for the Online Trajectory Scaling
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Gerelli, O., primary and Guarino Lo Bianco, C., additional
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- 2009
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10. Smooth profile generation for a tile printing machine
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Guarino Lo Bianco, C., primary and Zanasi, R., additional
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- 2003
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11. A simplified thermal analysis approach for power transistor rating in PWM-controlled DC/AC converters
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Filicori, F., primary and Guarino Lo Bianco, C., additional
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- 1998
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12. Circulating levels of soluble adhesion molecules in patients with ANCA-associated vasculitis
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Gabriele Di Lorenzo, Pacor, M. L., Mansueto, P., Lo Bianco, C., Di Natale, E., Rapisarda, F., Pellitteri, M. E., Ditta, V., Gioè, A., Giammarresi, G., Rini, G. B., Li Vecchi, M., DI LORENZO G, PACOR ML, MANSUETO P, LO BIANCO C, DI NATALE E, RAPISARDA F, PELLITTERI ME, DITTA V, GIOE A, GIAMMARRESI G, RINI GB, and LI VECCHI M
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Settore MED/09 - Medicina Interna ,soluble adhesion molecule ,ANCA-associated vasculitis ,soluble adhesion molecules - Abstract
BACKGROUND: During inflammation, activated vascular endothelial cells and other cell types express various adhesion molecules, which facilitate the binding of circulating leukocytes and their extravasation in surrounding tissue (i.e. renal tissue). The serum concentration of circulating soluble adhesion molecules is supposed to reflect the degree of this activation. OBJECTIVE: In the first part of the study, we determined if the serum levels of the soluble intercellular adhesion molecule (sICAM)-1 and the soluble endothelial cell-leukocyte adhesion molecule (sELAM)-1, in patients affected by microscopic polyangiitis (MPA), associated with myeloperoxidase (MPO)-anti-neutrophil cytoplasmic antibodies (ANCA), were related to the active and the inactive vasculitis phase. In the second part of the study, we examined the changes in circulating sICAM-1 and sELAM-1 levels and the clinical outcome of renal function in these patients. METHODS: We examined 20 MPO-ANCA-positive MPA patients in an acute phase and in a remission phase, after 6 months of treatment, and 50 subjects as controls, 30 with autosomal dominant polycystic kidney disease (ADPKD) in stable chronic renal failure (CRF) and 20 healthy volunteers (HS) with normal renal function. RESULTS: Regarding serum creatinine (Cr) concentration, no significant differences were found comparing active and inactive phases in the MPA group and the CRF group. Mean serum adhesion molecule levels in the MPA group were higher in the active phase compared to the inactive phase and to the CRF and HS groups. In addition, considering the outcome of serum Cr concentrations in the MPA group, the serum adhesion molecule levels were higher and decreased more slowly in patients with final high serum Cr concentrations than in patients with final normal serum Cr concentrations. CONCLUSION: Our data suggest that in MPO-ANCA-positive MPA patients, higher sICAM-1 and sELAM-1 levels during the active phase and their slower decline during the treatment period, could be a prognostic risk factor for CRF development.
13. Similarity and differences in elderly patients with fixed airflow obstruction by asthma and by chronic obstructive pulmonary disease
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Alberto D'Alcamo, Cristina Farina, Maria Esposito-Pellitteri, Francesco Gervasi, Gabriele Di Lorenzo, Gaetana Di Fede, Maria Stefania Leto-Barone, Calogero Caruso, Giovam Battista Rini, Vito Ditta, Claudia Lo Bianco, Pasquale Mansueto, DI LORENZO G, MANSUETO P, DITTA V, ESPOSITO-PELLITTERI M, LO BIANCO C, LETO-BARONE MS, D'ALCAMO A, FARINA C, DI FEDE G, GERVASI F, CARUSO C, and RINI G
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Male ,Pulmonary and Respiratory Medicine ,Vital capacity ,medicine.medical_specialty ,Settore MED/09 - Medicina Interna ,asthma, chronic obstructive pulmonary disease ,Neutrophils ,Vital Capacity ,chronic obstructive pulmonary disease ,Gastroenterology ,Pulmonary function testing ,Pulmonary Disease, Chronic Obstructive ,FEV1/FVC ratio ,Elderly ,DLCO ,Forced Expiratory Volume ,Internal medicine ,medicine ,Humans ,elderly patients ,fixed airflow obstruction ,asthma ,Aged ,Asthma ,COPD ,Eosinophil cationic protein ,business.industry ,Chronic obstructive pulmonary disease ,Respiratory disease ,Sputum ,medicine.disease ,Respiratory Function Tests ,elderly patient ,respiratory tract diseases ,Surgery ,Eosinophils ,Female ,business - Abstract
SummaryBackgroundEpidemiologic studies have demonstrated that elderly patients with fixed airflow obstruction can be affected by asthma or chronic obstructive pulmonary disease (COPD).MethodsWe studied 49 consecutive elderly outpatients, presenting fixed airflow obstruction, by clinical history (smoking), pulmonary function tests, blood gas analysis, and induced sputum.ResultsThe age was not different in patients with COPD (n=28) and asthma (n=21) (70.2±3.9 years vs. 69.6±3.7 years), also the degree of fixed airflow obstruction was similar (FEV1: 58.3±1.5% vs. 59.0±1.4% of predicted). Patients with asthma had significantly more eosinophils in peripheral blood (0.43±0.05×10−3μL vs. 0.27±0.1×10−3μL, P
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- 2008
14. Is there a role for antileukotrienes in urticaria?
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Giovam Battista Rini, Vito Ditta, Maria Stefania Leto-Barone, C. Lo Bianco, Pasquale Mansueto, G. Di Lorenzo, M. L. Pacor, Maria Esposito-Pellitteri, G. Di Fede, DI LORENZO G, PACOR ML, MANSUETO P, ESPOSITO-PELLITTERI M, DITTA V, LO BIANCO C, LETO-BARONE MS, DI FEDE G, and RINI GB
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medicine.medical_specialty ,Allergy ,Leukotrienes ,Settore MED/09 - Medicina Interna ,Urticaria ,medicine.medical_treatment ,Dermatology ,Cold urticaria ,immune system diseases ,parasitic diseases ,medicine ,Humans ,Zafirlukast ,skin and connective tissue diseases ,Montelukast ,Asthma ,Clinical Trials as Topic ,Aspirin ,business.industry ,Anti-Inflammatory Agents, Non-Steroidal ,Zileuton ,medicine.disease ,Antileukotriene ,antileukotriene ,antileukotrienes ,urticaria ,Histamine H1 Antagonists ,Leukotriene Antagonists ,Antihistamine ,Drug Therapy, Combination ,Food Additives ,business ,medicine.drug - Abstract
In vitro and in vivo clinical and experimental data have suggested that leukotrienes play a key role in inflammatory reactions of the skin. Antileukotriene drugs, i.e. leukotriene receptor antagonists and synthesis inhibitors, are a new class of anti-inflammatory drugs that have shown clinical efficacy in the management of asthma. We searched the MedLine database and carried out a manual search on journals specializing in allergy and dermatology for the use of antileukotriene drugs in urticaria. Montelukast might be effective in chronic urticaria associated with aspirin or food additive hypersensitivity or with autoreactivity to intradermal serum injection when taken with an antihistamine but not in moderate chronic idiopathic urticaria. Evidence for the effectiveness of zafirlukast and the 5-lipoxygenase inhibitor, zileuton, in chronic urticaria is mainly anecdotal. In addition, there is anecdotal evidence of effectiveness of antileukotrienes in primary cold urticaria, delayed pressure urticaria and dermographism. No evidence exists for other physical urticarias, including cholinergic, solar and aquagenic urticarias, vibratory angio-oedema, and exercise-induced anaphylaxis.
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- 2006
15. Randomized placebo-controlled trial comparing fluticasone aqueous nasal spray in mono-therapy, fluticasone plus cetirizine, fluticasone plus montelukast and cetirizine plus montelukast for seasonal allergic rhinitis
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C. Lo Bianco, Morici G, Roberto Corrocher, Giuseppina Candore, Vito Ditta, Rini Gb, M. L. Pacor, Maria Esposito Pellitteri, Calogero Caruso, G. Di Lorenzo, Nicola Martinelli, Pasquale Mansueto, A. Di Gregoli, DI LORENZO G, PACOR ML, PELLITTERI ME, MORICI G, DI GREGOLI A, LO BIANCO C, DITTA V, MARTINELLI N, CANDORE G, MANSUETO P, RINI GB, CORROCHER R, and CARUSO C
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Cyclopropanes ,Male ,Allergy ,Settore MED/09 - Medicina Interna ,medicine.medical_treatment ,seasonal allergic rhinitis ,Acetates ,Gastroenterology ,Immunology and Allergy ,Medicine ,Child ,Fluticasone ,pollen season ,Randomized placebo-controlled trial ,Blood Proteins ,respiratory system ,Eosinophil Granule Proteins ,Middle Aged ,Cetirizine ,Anesthesia ,montelukast ,Histamine H1 Antagonists ,Quinolines ,eosinophil cationic protein ,Drug Therapy, Combination ,Female ,eosinophils ,medicine.symptom ,medicine.drug ,Adult ,medicine.medical_specialty ,Adolescent ,Immunology ,Nasal congestion ,Sulfides ,Placebo ,Fluticasone propionate ,Drug Administration Schedule ,Ribonucleases ,Double-Blind Method ,Internal medicine ,Humans ,fluticasone ,cetirizine ,Glucocorticoids ,Montelukast ,Administration, Intranasal ,Analysis of Variance ,rhinorrhea ,fluticasone propionate ,business.industry ,nasal lavage ,Rhinitis, Allergic, Seasonal ,medicine.disease ,Androstadienes ,Parietaria ,Nasal spray ,Leukotriene Antagonists ,Nasal administration ,business - Abstract
BACKGROUND: Corticosteroids are considered to be particularly effective in reducing nasal congestion and are therefore recommended as first-line treatment in allergic rhinitis patients with moderate to severe and/or persistent symptoms. OBJECTIVE: We compared the clinical efficacy of fluticasone propionate aqueous nasal spray (FPANS) 200 microg given once daily, administered in mono-therapy or combined therapy with a H1 receptor antagonist (cetirizine, CTZ) or with a leukotriene antagonist (montelukast, MSK), and the combined therapy of CTZ plus MSK in the treatment of patients affected by allergic rhinitis to Parietaria during natural pollen exposure. In addition, we examined the effect of the treatment on eosinophil counts and eosinophil cationic protein (ECP) in nasal lavage performed at beginning of season, during season and at the end of the season. METHODS: One hundred patients aged 12-50 years (mean+/-SD 31.8+/-9.6) with a history of moderate to severe Parietaria pollen-induced seasonal allergic rhinitis were selected. A randomized, double-blind, double dummy, placebo (PLA)-controlled, parallel-group study design was used. Patients were treated FPANS 200 microg once daily (n=20) or with FPANS 200 microg once daily, plus CTZ (10 mg) in the morning (n=20), or with FPANS 200 microg once daily, plus MSK (10 mg) in the evening (n=20) or with CTZ (10 mg) in the morning plus MSK in the evening (n=20) or matched PLA (n=20). Assessment of efficacy was based on scores of daily nasal symptoms and on eosinophil counts and ECP in nasal lavage. RESULTS: All treatments showed significant differences (P
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- 2004
16. Randomized placebo-controlled trial comparing desloratadine and montelukast in monotherapy and desloratadine plus montelukast in combined therapy for chronic idiopathic urticaria
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Pasquale Mansueto, Gabriele Di Lorenzo, Claudia Lo Bianco, Maria Esposito Pellitteri, Nicola Martinelli, Giovam Battista Rini, A. Vito Ditta, Maria Luisa Pacor, DI LORENZO G, PACOR ML, MANSUETO P, ESPOSITO PELLITTERI M, LO BIANCO C, DITTA V, MARTINELLI N, and RINI GB
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Adult ,Cyclopropanes ,Male ,medicine.medical_specialty ,Histamine H1 Antagonists, Non-Sedating ,Settore MED/09 - Medicina Interna ,Adolescent ,Urticaria ,medicine.medical_treatment ,Immunology ,Placebo-controlled study ,Randomized placebo-controlled trial ,desloratadine ,montelukast ,chronic idiopathic urticaria ,Acetates ,Sulfides ,Placebo ,Gastroenterology ,law.invention ,chemistry.chemical_compound ,Randomized controlled trial ,Double-Blind Method ,law ,Internal medicine ,medicine ,Immunology and Allergy ,Humans ,Montelukast ,Aged ,Desloratadine ,Leukotriene E4 ,Leukotriene receptor ,business.industry ,Loratadine ,Middle Aged ,Antileukotriene ,Treatment Outcome ,chemistry ,Anesthesia ,Chronic Disease ,Quinolines ,Leukotriene Antagonists ,Female ,business ,medicine.drug - Abstract
BACKGROUND: H 1 -receptor antagonists are considered to be particularly effective in reducing pruritus, and they are therefore recommended as first-line treatment in patients with chronic idiopathic urticaria (CIU). Recently, antileukotriene receptors have been used in patients with CIU, either administered as monotherapy or combined with H 1 -receptor antagonists. OBJECTIVE: We compared the clinical efficacy of 5 mg of desloratadine administered once daily either as monotherapy or combined with a leukotriene antagonist, 10 mg of montelukast daily, and 10 mg of montelukast administered daily as monotherapy for the treatment of patients affected by CIU with placebo. METHODS: One hundred sixty patients aged 18 to 69 years (mean +/- SD, 43.9 +/- 13.4 years) with a history of moderate CIU were selected. A randomized, double-blind, double-dummy, placebo-controlled, parallel-group study design was used. Patients were treated with 5 mg of desloratadine once daily (n = 40), 10 mg of montelukast once daily (n = 40), 5 mg of desloratadine (n = 40) in the morning plus montelukast in the evening, or matched placebo (n = 40). Assessment of treatment efficacy was based on scores of daily cutaneous symptoms evaluated reflectively and instantaneously. RESULTS: Only the group treated with desloratadine as monotherapy or as combined therapy concluded the whole study. Twenty-seven of the 40 patients in the montelukast group and 35 of the 40 patients in the placebo group discontinued the treatment. As reflective evaluation, all groups showed significant differences compared with the placebo group in terms of total symptom score, number of hives, and size of largest hive. In addition to the pruritus, only the groups treated with desloratadine as monotherapy or combined therapy showed significant differences compared with those receiving placebo, whereas there were no differences between the montelukast and placebo groups. Finally, no differences were found between the desloratadine group and the desloratadine plus montelukast group. The instantaneous evaluation demonstrated similar results regarding the desloratadine group and the desloratadine plus montelukast group versus the placebo group, whereas there were no significant differences between the group treated with montelukast alone and the placebo group for pruritus and size of largest hive. No differences were found between the group treated with desloratadine alone and the desloratadine plus montelukast group. CONCLUSIONS: The results of this comparative study demonstrate that desloratadine is highly effective for the treatment of patients affected by CIU. In addition, the regular combined therapy of desloratadine plus montelukast does not seem to offer a substantial advantage with respect to desloratadine as monotherapy in patients affected by moderate CIU.
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- 2004
17. The characteristics of different diagnostic tests in adult mild asthmatic patients: Comparison with patients with asthma-like symptoms by gastro-oesophageal reflux
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Gabriele Di Lorenzo, Giovam Battista Rini, Maria Esposito-Pellitteri, Maria Stefania Leto-Barone, Sergio Vigneri, F. Castello, Pasquale Mansueto, Marcello Traverso, Vito Ditta, Giuseppe Rotolo, Gaetana Di Fede, Claudia Lo Bianco, DI LORENZO G, MANSUETO P, ESPOSITO-PELLITTERI M, DITTA V, CASTELLO F, LO BIANCO C, LETO-BARONE MS, DI FEDE G, TRAVERSO M, ROTOLO G, VIGNERI S, and RINI G
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Male ,Vital capacity ,Settore MED/09 - Medicina Interna ,adult mild asthmatic patient ,Vital Capacity ,Gastroenterology ,gastro-oesophageal reflux ,Bronchoconstrictor Agents ,Leukocyte Count ,Sensitivity ,immune system diseases ,Forced Expiratory Volume ,Methacholine Chloride ,Eosinophil cationic protein ,Respiratory disease ,Middle Aged ,respiratory system ,medicine.anatomical_structure ,Diagnostic tests ,Gastroesophageal Reflux ,Specificity ,Female ,medicine.drug ,circulatory and respiratory physiology ,Adult ,Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Bronchial Provocation Tests ,Diagnosis, Differential ,FEV1/FVC ratio ,Gastro-oesophageal reflux disease ,Internal medicine ,medicine ,Humans ,Asthma ,business.industry ,Eosinophil Cationic Protein ,Sputum ,adult mild asthmatic patients ,Eosinophil ,medicine.disease ,Surgery ,respiratory tract diseases ,Eosinophils ,Spirometry ,GERD ,Methacholine ,Epidemiologic Methods ,business ,Biomarkers ,Gastro-oesophageal reflux - Abstract
SummaryBackgroundDiagnosing asthma cannot be always easy. It is important to consider the validity of the diagnostic tests, and/or how much more commonly they are positive in patients with asthma compared to healthy subjects and, particularly, to patients with asthma-like symptoms.ObjectiveTo evaluate the validity of diagnostic tests for asthma, in terms of sensitivity, specificity, positive and negative predictive values, in patients with bronchial asthma compared to patients affected by gastro-oesophageal reflux disease (GERD) with asthma-like symptoms, and healthy control subjects without asthma and gastro-oesophageal reflux (GER).DesignSingle-center, cross-sectional, observational study.PatientsWe studied 60 patients with mild asthma, 30 patients with GERD and asthma-like symptoms and 25 healthy control subjects.MeasurementsWe measured provocative concentration of methacholine causing a 20% fall in the forced expiratory volume in 1s (MCh PC20/FEV1), the amplitude percent mean of peak expiratory flow (A%M of PEF), derived from twice-daily readings for >2 weeks, the FEV1/forced vital capacity (FEV1/FVC) ratio, the eosinophil count in blood and in induced sputum and the serum eosinophil cationic protein (ECP) levels.ResultsFEV1/FVC ratio, A%M of PEF, blood eosinophils counts and serum ECP levels were less sensitive and specific when the reference population was composed of patients with asthma-like symptoms by GER. While, MCh PC20/FEV1 and induced sputum eosinophils count were the most sensitive (both 90%) and specific (89% and 92%, respectively) tests.ConclusionOur findings demonstrate that MCh PC20/FEV1 and the induced sputum eosinophil count are the most useful objective tests in patients with mild asthma. All patients with asthma presented both an MCh PC20/FEV1 1%.
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18. Food allergy in gastroenterologic diseases: Review of literature
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Maria Esposito-Pellitteri, Giuseppe Montalto, Pasquale Mansueto, Stefania Maria Leto-Barone, Maria Luisa Pacor, Claudia Lo Bianco, Gabriele Di Lorenzo, Vito Ditta, Mansueto, P., Montalto, G., Pacor, M., ESPOSITO PELLITTERI, M., Ditta, V., LO BIANCO, C., LETO BARONE, S., and DI LORENZO, G.
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medicine.medical_specialty ,Allergy ,Settore MED/09 - Medicina Interna ,Epinephrine ,Gastrointestinal Diseases ,Food allergy ,gastroenterologic diseases ,Population ,Review ,Immunoglobulin E ,Oral allergy syndrome ,medicine ,Humans ,Adverse effect ,education ,Anaphylaxis ,Skin Tests ,education.field_of_study ,biology ,business.industry ,Oral food challenge ,digestive, oral, and skin physiology ,Gastroenterology ,General Medicine ,medicine.disease ,Dermatology ,Immunology ,Histamine H1 Antagonists ,biology.protein ,Immunotherapy ,business ,Food Hypersensitivity - Abstract
Food allergy is a common and increasing problem worldwide. The newly-found knowledge might provide novel experimental strategies, especially for laboratory diagnosis. Approximately 20% of the population alters their diet for a perceived adverse reaction to food, but the application of double-blind placebo-controlled oral food challenge, the “gold standard” for diagnosis of food allergy, shows that questionnaire-based studies overestimate the prevalence of food allergies. The clinical disorders determined by adverse reactions to food can be classified on the basis of immunologic or nonimmunologic mechanisms and the organ system or systems affected. Diagnosis of food allergy is based on clinical history, skin prick tests, and laboratory tests to detect serum-food specific IgE, elimination diets and challenges. The primary therapy for food allergy is to avoid the responsible food. Antihistamines might partially relieve oral allergy syndrome and IgE-mediated skin symptoms, but they do not block systemic reactions. Systemic corticosteroids are generally effective in treating chronic IgE-mediated disorders. Epinephrine is the mainstay of treatment for anaphylaxis. Experimental therapies for IgE-mediated food allergy have been evaluated, such as humanized IgG anti-IgE antibodies and allergen specific immunotherapy.
19. Relationship between specific serum IGE to Ascaris lumbricoides and onset of respiratory symptoms in Bangladesh immigrants
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Serafino Mansueto, Maria Esposito-Pellitteri, G. Di Fede, Nicola Scichilone, Roberto Corrocher, M. L. Pacor, Vito Ditta, Maria Stefania Leto-Barone, Pasquale Mansueto, C. Lo Bianco, Rini Gb, G. Di Lorenzo, DI LORENZO G, PACOR ML, MANSUETO P, ESPOSITO-PELLITTERI M, SCICHILONE NA, DITTA V, LO BIANCO C, LETO-BARONE MS, DI FEDE G, CORROCHER R, MANSUETO S, and RINI GB
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Adult ,Male ,Settore MED/09 - Medicina Interna ,Rhinitis, Allergic, Perennial ,030231 tropical medicine ,Immunology ,Antibodies, Helminth ,Immunoglobulin E ,Serum ige ,030308 mycology & parasitology ,specific serum IgE ,Allergic sensitization ,03 medical and health sciences ,0302 clinical medicine ,Air Pollution ,Immunology and Allergy ,Medicine ,Animals ,Humans ,Respiratory system ,Ascaris lumbricoides ,Asthma ,Skin Tests ,Pharmacology ,0303 health sciences ,Family Characteristics ,biology ,business.industry ,Ascaris ,Bangladesh immigrants ,Rhinitis, Allergic, Seasonal ,Hygiene ,Emigration and Immigration ,biology.organism_classification ,medicine.disease ,Logistic Models ,biology.protein ,Ascaris lumbricoide ,Female ,Antibody ,business - Abstract
The role of helminths in asthma and/or rhinitis and in allergic sensitization is still unclear. We assessed the relationship between Ascaris-specific IgE, respiratory symptoms and allergic sensitization in Bangladesh immigrants. 246 individuals were examined from 1996 to 2001. Serum total IgE, Ascaris IgE, specific IgE to inhalant allergens, skin prick tests (SPT) and parasitological evaluation of the stool were performed. Total serum IgE were significantly higher in Ascaris-IgE positive (> 0.35 kU/L) individuals (806.5 [409.0–1436.0] kU/L vs. 207.0 [127.0–332.5] kU/L; P < 0.0001) and in subjects with respiratory symptoms (413.0 [239.0–1096.0] kU/L vs. 259.5 [147.0–387.0] kU/L), ( P < 0.0001), but not in SPT positive subjects (413.0 [179.0–894.0] kU/L vs. 404.6 [305.0–1201.0] kU/L ( P= 0.5). Ascaris-specific IgE were detected in 48 subjects with respiratory symptoms (40.0%) and in 46 subjects without respiratory symptoms (36.5%) ( P = 0.5). The SPT positivity was similar between Ascaris-IgE seropositive (38.2%) and Ascaris-IgE seronegative (38.1%) subjects ( P = 0.9). Total IgE and length of stay in Italy correlated with SPT positivity (OR 5.6 [CI 95% 1.5–19.8], P = 0.007, and OR 1.5 [CI 95% 1.3–1.7], P < 0.0001), and with respiratory symptoms (OR 13.7 [CI 95% 3.0–62.4], P = 0.0007, and OR 2.4 [CI 95% 1.9–3.0], P < 0.0001). Ascaris-IgE were negatively associated with SPT positivity (OR 0.3 [CI 95% 0.1–0.8], P = 0.02) and with respiratory symptoms (OR 0.1 [CI 95% 0.04–0.7], P = 0.01). Our findings favour the role of environmental factors in the development of respiratory symptoms in immigrants, irrespective of Ascaris-IgE.
20. Determinants of bronchial hyperresponsiveness in subjects with rhinitis
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M. L. Pacor, G. Di Lorenzo, Pasquale Mansueto, Maria Stefania Leto-Barone, Vito Ditta, Giovam Battista Rini, G. Di Fede, C. Lo Bianco, Nicola Napoli, M. Esposito Pellitteri, DI LORENZO G, PACOR ML, MANSUETO P, ESPOSITO PELLITTERI M, LO BIANCO C, DITTA V, LETO-BARONE MS, NAPOLI N, DI FEDE G, and RINI GB
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Adult ,Male ,Vital capacity ,medicine.medical_specialty ,Immunology ,Vital Capacity ,Gastroenterology ,Group B ,03 medical and health sciences ,FEV1/FVC ratio ,Leukocyte Count ,0302 clinical medicine ,Internal medicine ,Forced Expiratory Volume ,medicine ,Immunology and Allergy ,Humans ,Life Style ,BRONCHIAL HYPERRESPONSIVENESS ,RHINITIS ,Asthma ,Rhinitis ,Skin Tests ,Pharmacology ,business.industry ,Rhinitis, Allergic, Seasonal ,Passive smoke ,Immunoglobulin E ,medicine.disease ,Respiratory Function Tests ,Eosinophils ,Bronchial hyperresponsiveness ,Spirometry ,030220 oncology & carcinogenesis ,Methacholine ,Female ,Geometric mean ,Bronchial Hyperreactivity ,business ,030215 immunology ,medicine.drug - Abstract
Subjects with rhinitis but without asthma may have coexisting bronchial hyperresponsiveness, although the reasons for this are uncertain. To evaluate the factors that determine BHR in rhinitis we examined 410 patients with symptomatic rhinitis with forced expiratory volume in 1 second (FEV1) and forced vital capacity (FVC) ≥ 80% of the predicted value. In all subjects a skin prick test (SPT) was performed, a determination of total serum IgE and an eosinophils count in the blood. Of the 410 subjects we found that 161 (39.3%) exhibited a methacholine PD20 of 800 mg or less (Group A), whereas 249 (60.7%) had a methacholine PD20 more of 800 mg (Group B). Despite the matched mean values for FEV1 and FVC, compared with Group B, Group A had a lower predicted forced expiratory flow between 25% and 75% (FEF25%-75%) (86.7 ± 12.0 vs. 93.7±7.3, P < 0.0001). A great portion of the subjects of the Group A in respect to subjects of the Group B were exposed to passive smoke (37.8% vs. 22.0%, P = 0.0008), reported having mothers with asthma (34.1% vs. 6.0%, P < 0.0001), presented a positive skin prick test (93.7% vs. 67.0%, P < 0.0001), had higher levels of total serum IgE (geometric mean of Log10 2.46 ± 0.27 kU/L vs. 2.06 ± 0.38 kU/L, P < 0.0001) and higher blood eosinophil counts (geometric mean of Log10 2.67 ± 0.07 × 10−3 mL vs. 2.57 ± 0.09 × 10−3 mL, P < 0.0001), and reported increased nasal obstruction (2.0 (95%CI 1.8 to 2.2) vs. 0.6 (95%CI 0.5 to 0.7), P < 0.0001). Logistic regression demonstrates that nasal obstruction (OR 2.19,95%CI 1.72 to 2.80) and the presence of positive SPT (OR 6.15,95%CI 2.42 to 15.61) were the most available predictors to discriminate between subjects with BHR and subjects without BHR. In addition, BHR was positively related to blood eosinophil counts (OR= 2.80, 95%CI 1.54 to 5.07), FEF25%-75% values (OR= 2.72, 95%CI 1.23 to 5.99) and familiarity (mother) for asthma (OR = 2.45, 95%CI 1.10 to 5.46). Whereas passive smoke and total serum IgE were not positively related to BHR. Increased nasal obstruction and the presence of positive SPT were the most available predictors to discriminate between subjects with and without BHR. Finally, BHR was positively related to blood eosinophil counts, FEF25%-75% values and to familiarity (mother) for asthma.
21. Glucocerebrosidase deficiency in dopaminergic neurons induces microglial activation without neurodegeneration.
- Author
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Soria FN, Engeln M, Martinez-Vicente M, Glangetas C, López-González MJ, Dovero S, Dehay B, Normand E, Vila M, Favereaux A, Georges F, Lo Bianco C, Bezard E, and Fernagut PO
- Subjects
- Animals, Brain metabolism, Gaucher Disease genetics, Gaucher Disease metabolism, Genetic Vectors, Mesencephalon metabolism, Mice, Mice, Knockout, Microglia metabolism, Models, Animal, Parkinson Disease genetics, Parkinson Disease metabolism, Substantia Nigra metabolism, alpha-Synuclein metabolism, Dopaminergic Neurons metabolism, Glucosylceramidase genetics, Glucosylceramidase metabolism
- Abstract
Mutations in the GBA1 gene encoding the lysosomal enzyme glucocerebrosidase (GBA1) are important risk factors for Parkinson's disease (PD). In vitro, altered GBA1 activity promotes alpha-synuclein accumulation whereas elevated levels of alpha-synuclein compromise GBA1 function, thus supporting a pathogenic mechanism in PD. However, the mechanisms by which GBA1 deficiency is linked to increased risk of PD remain elusive, partially because of lack of aged models of GBA1 deficiency. As knocking-out GBA1 in the entire brain induces massive neurodegeneration and early death, we generated a mouse model of GBA1 deficiency amenable to investigate the long-term consequences of compromised GBA1 function in dopaminergic neurons. DAT-Cre and GBA1-floxed mice were bred to obtain selective homozygous disruption of GBA1 in midbrain dopamine neurons (DAT-GBA1-KO). Mice were followed for motor function, neuronal survival, alpha-synuclein phosphorylation and glial activation. Susceptibility to nigral viral vector-mediated overexpression of mutated (A53T) alpha-synuclein was assessed. Despite loss of GBA1 and substrate accumulation, DAT-GBA1-KO mice displayed normal motor performances and preserved dopaminergic neurons despite robust microglial activation in the substantia nigra, without accumulation of endogenous alpha-synuclein with respect to wild-type mice. Lysosomal function was only marginally affected. Screening of micro-RNAs linked to the regulation of GBA1, alpha-synuclein or neuroinflammation did not reveal significant alterations. Viral-mediated overexpression of A53T-alpha-synuclein yielded similar neurodegeneration in DAT-GBA1-KO mice and wild-type mice. These results indicate that loss of GBA1 function in mouse dopaminergic neurons is not critical for alpha-synuclein accumulation or neurodegeneration and suggest the involvement of GBA1 deficiency in other cell types as a potential mechanism., (© The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Published
- 2017
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22. Hsp104 antagonizes alpha-synuclein aggregation and reduces dopaminergic degeneration in a rat model of Parkinson disease.
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Lo Bianco C, Shorter J, Régulier E, Lashuel H, Iwatsubo T, Lindquist S, and Aebischer P
- Subjects
- Amyloid chemistry, Animals, Brain metabolism, Disease Models, Animal, HSP40 Heat-Shock Proteins chemistry, HSP70 Heat-Shock Proteins metabolism, Heat-Shock Proteins chemistry, Humans, Models, Biological, Neurodegenerative Diseases metabolism, Protein Binding, Rats, Dopamine metabolism, Heat-Shock Proteins physiology, Parkinson Disease metabolism, alpha-Synuclein chemistry
- Abstract
Parkinson disease (PD) is characterized by dopaminergic neurodegeneration and intracellular inclusions of alpha-synuclein amyloid fibers, which are stable and difficult to dissolve. Whether inclusions are neuroprotective or pathological remains controversial, because prefibrillar oligomers may be more toxic than amyloid inclusions. Thus, whether therapies should target inclusions, preamyloid oligomers, or both is a critically important issue. In yeast, the protein-remodeling factor Hsp104 cooperates with Hsp70 and Hsp40 to dissolve and reactivate aggregated proteins. Metazoans, however, have no Hsp104 ortholog. Here we introduced Hsp104 into a rat PD model. Remarkably, Hsp104 reduced formation of phosphorylated alpha-synuclein inclusions and prevented nigrostriatal dopaminergic neurodegeneration induced by PD-linked alpha-synuclein (A30P). An in vitro assay employing pure proteins revealed that Hsp104 prevented fibrillization of alpha-synuclein and PD-linked variants (A30P, A53T, E46K). Hsp104 coupled ATP hydrolysis to the disassembly of preamyloid oligomers and amyloid fibers composed of alpha-synuclein. Furthermore, the mammalian Hsp70 and Hsp40 chaperones, Hsc70 and Hdj2, enhanced alpha-synuclein fiber disassembly by Hsp104. Hsp104 likely protects dopaminergic neurons by antagonizing toxic alpha-synuclein assemblies and might have therapeutic potential for PD and other neurodegenerative amyloidoses.
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- 2008
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23. Similarity and differences in elderly patients with fixed airflow obstruction by asthma and by chronic obstructive pulmonary disease.
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Di Lorenzo G, Mansueto P, Ditta V, Esposito-Pellitteri M, Lo Bianco C, Leto-Barone MS, D'Alcamo A, Farina C, Di Fede G, Gervasi F, Caruso C, and Rini G
- Subjects
- Aged, Asthma physiopathology, Eosinophils, Female, Forced Expiratory Volume, Humans, Male, Pulmonary Disease, Chronic Obstructive physiopathology, Respiratory Function Tests, Sputum cytology, Vital Capacity, Asthma diagnosis, Pulmonary Disease, Chronic Obstructive diagnosis
- Abstract
Background: Epidemiologic studies have demonstrated that elderly patients with fixed airflow obstruction can be affected by asthma or chronic obstructive pulmonary disease (COPD)., Methods: We studied 49 consecutive elderly outpatients, presenting fixed airflow obstruction, by clinical history (smoking), pulmonary function tests, blood gas analysis, and induced sputum., Results: The age was not different in patients with COPD (n=28) and asthma (n=21) (70.2+/-3.9 years vs. 69.6+/-3.7 years), also the degree of fixed airflow obstruction was similar (FEV1: 58.3+/-1.5% vs. 59.0+/-1.4% of predicted). Patients with asthma had significantly more eosinophils in peripheral blood (0.43+/-0.05x10(-3)microL vs. 0.27+/-0.1x10(-3)microL, P<0.0001), and in induced sputum (5.0% [(p25th and p75th) 5.0-6.0%] vs. 1.0% [(p25th and p75th) 0.01-1.0%]; P<0.0001), as well as serum ECP (18.6+/-4.9ng/mL vs. 7.7+/-4.7ng/mL, P<0.0001) and ECP in the induced sputum (31.6+/-2.9ng/mL vs. 5.6+/-4.9ng/mL, P<0.0001). Finally, in induced sputum the eosinophils EG2+ were higher in patients with asthma than in patients with COPD (40.5 [(p25th and p75th) 39.3-44.3] MFI vs. 3.9 [(p25th and p75th) 0-11.4] MFI, P<0.0001). They also had significantly higher diffusing capacity, and a greater reversibility to steroids, after 14-day course of therapy, whereas the reversibility to 400microg of salbutamol was similar., Conclusion: Despite similar fixed airflow obstruction, elderly patients with asthma have distinct characteristics compared with patients with COPD.
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- 2008
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24. Murine models of acute neuronopathic Gaucher disease.
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Enquist IB, Lo Bianco C, Ooka A, Nilsson E, Månsson JE, Ehinger M, Richter J, Brady RO, Kirik D, and Karlsson S
- Subjects
- Animals, Biomarkers, Cell Proliferation, Disease Progression, Gaucher Disease genetics, Glucosylceramidase deficiency, Glucosylceramidase genetics, Glucosylceramidase metabolism, Intermediate Filament Proteins metabolism, Introns genetics, Mice, Mice, Transgenic, Microglia metabolism, Microglia pathology, Nerve Tissue Proteins metabolism, Nestin, RNA Splicing genetics, Disease Models, Animal, Gaucher Disease metabolism, Gaucher Disease pathology
- Abstract
Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the glucosidase, beta, acid (GBA) gene that encodes the lysosomal enzyme glucosylceramidase (GCase). GCase deficiency leads to characteristic visceral pathology and, in some patients, lethal neurological manifestations. Here, we report the generation of mouse models with the severe neuronopathic form of GD. To circumvent the lethal skin phenotype observed in several of the previous GCase-deficient animals, we genetically engineered a mouse model with strong reduction in GCase activity in all tissues except the skin. These mice exhibit rapid motor dysfunction associated with severe neurodegeneration and apoptotic cell death within the brain, reminiscent of neuronopathic GD. In addition, we have created a second mouse model, in which GCase deficiency is restricted to neural and glial cell progenitors and progeny. These mice develop similar pathology as the first mouse model, but with a delayed onset and slower disease progression, which indicates that GCase deficiency within microglial cells that are of hematopoietic origin is not the primary determinant of the CNS pathology. These findings also demonstrate that normal microglial cells cannot rescue this neurodegenerative disease. These mouse models have significant implications for the development of therapy for patients with neuronopathic GD.
- Published
- 2007
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25. The characteristics of different diagnostic tests in adult mild asthmatic patients: comparison with patients with asthma-like symptoms by gastro-oesophageal reflux.
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Di Lorenzo G, Mansueto P, Esposito-Pellitteri M, Ditta V, Castello F, Lo Bianco C, Leto-Barone MS, Di Fede G, Traverso M, Rotolo G, Vigneri S, and Rini G
- Subjects
- Adult, Asthma physiopathology, Biomarkers blood, Bronchial Provocation Tests methods, Bronchoconstrictor Agents, Diagnosis, Differential, Eosinophil Cationic Protein blood, Eosinophils pathology, Epidemiologic Methods, Female, Forced Expiratory Volume, Gastroesophageal Reflux physiopathology, Humans, Leukocyte Count, Male, Methacholine Chloride, Middle Aged, Spirometry methods, Sputum cytology, Vital Capacity, Asthma diagnosis, Gastroesophageal Reflux diagnosis
- Abstract
Background: Diagnosing asthma cannot be always easy. It is important to consider the validity of the diagnostic tests, and/or how much more commonly they are positive in patients with asthma compared to healthy subjects and, particularly, to patients with asthma-like symptoms., Objective: To evaluate the validity of diagnostic tests for asthma, in terms of sensitivity, specificity, positive and negative predictive values, in patients with bronchial asthma compared to patients affected by gastro-oesophageal reflux disease (GERD) with asthma-like symptoms, and healthy control subjects without asthma and gastro-oesophageal reflux (GER)., Design: Single-center, cross-sectional, observational study., Patients: We studied 60 patients with mild asthma, 30 patients with GERD and asthma-like symptoms and 25 healthy control subjects., Measurements: We measured provocative concentration of methacholine causing a 20% fall in the forced expiratory volume in 1s (MCh PC(20)/FEV(1)), the amplitude percent mean of peak expiratory flow (A%M of PEF), derived from twice-daily readings for >2 weeks, the FEV(1)/forced vital capacity (FEV(1)/FVC) ratio, the eosinophil count in blood and in induced sputum and the serum eosinophil cationic protein (ECP) levels., Results: FEV(1)/FVC ratio, A%M of PEF, blood eosinophils counts and serum ECP levels were less sensitive and specific when the reference population was composed of patients with asthma-like symptoms by GER. While, MCh PC(20)/FEV(1) and induced sputum eosinophils count were the most sensitive (both 90%) and specific (89% and 92%, respectively) tests., Conclusion: Our findings demonstrate that MCh PC(20)/FEV(1) and the induced sputum eosinophil count are the most useful objective tests in patients with mild asthma. All patients with asthma presented both an MCh PC(20)/FEV(1) <1500 microg and eosinophils count in the induced sputum >1%.
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- 2007
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26. Food allergy in gastroenterologic diseases: Review of literature.
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Mansueto P, Montalto G, Pacor ML, Esposito-Pellitteri M, Ditta V, Lo Bianco C, Leto-Barone SM, and Di Lorenzo G
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- Anaphylaxis drug therapy, Epinephrine therapeutic use, Food Hypersensitivity diagnosis, Food Hypersensitivity therapy, Gastrointestinal Diseases classification, Gastrointestinal Diseases immunology, Gastrointestinal Diseases prevention & control, Histamine H1 Antagonists therapeutic use, Humans, Immunoglobulin E blood, Immunotherapy, Skin Tests, Food Hypersensitivity complications, Food Hypersensitivity immunology, Gastrointestinal Diseases etiology
- Abstract
Food allergy is a common and increasing problem worldwide. The newly-found knowledge might provide novel experimental strategies, especially for laboratory diagnosis. Approximately 20% of the population alters their diet for a perceived adverse reaction to food, but the application of double-blind placebo-controlled oral food challenge, the "gold standard" for diagnosis of food allergy, shows that questionnaire-based studies overestimate the prevalence of food allergies. The clinical disorders determined by adverse reactions to food can be classified on the basis of immunologic or nonimmunologic mechanisms and the organ system or systems affected. Diagnosis of food allergy is based on clinical history, skin prick tests, and laboratory tests to detect serum-food specific IgE, elimination diets and challenges. The primary therapy for food allergy is to avoid the responsible food. Antihistamines might partially relieve oral allergy syndrome and IgE-mediated skin symptoms, but they do not block systemic reactions. Systemic corticosteroids are generally effective in treating chronic IgE-mediated disorders. Epinephrine is the mainstay of treatment for anaphylaxis. Experimental therapies for IgE-mediated food allergy have been evaluated, such as humanized IgG anti-IgE antibodies and allergen specific immunotherapy.
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- 2006
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27. Lentiviral vector delivery of parkin prevents dopaminergic degeneration in an alpha-synuclein rat model of Parkinson's disease.
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Lo Bianco C, Schneider BL, Bauer M, Sajadi A, Brice A, Iwatsubo T, and Aebischer P
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- Alanine genetics, Alanine metabolism, Animals, Brain metabolism, Brain pathology, Disease Models, Animal, Female, Gene Expression, Genetic Vectors genetics, Mutation genetics, Nerve Tissue Proteins genetics, Neurons metabolism, Neurons pathology, Parkinson Disease genetics, Parkinson Disease therapy, Phosphorylation, Rats, Rats, Wistar, Synucleins, Ubiquitin-Protein Ligases genetics, alpha-Synuclein, Dopamine metabolism, Genetic Therapy, Lentivirus genetics, Nerve Tissue Proteins metabolism, Parkinson Disease metabolism, Parkinson Disease pathology, Ubiquitin-Protein Ligases metabolism
- Abstract
Parkinson's disease (PD) is characterized by a progressive loss of midbrain dopamine neurons and the presence of cytoplasmic inclusions called Lewy bodies. Mutations in several genes including alpha-synuclein and parkin have been linked to familial PD. The loss of parkin's E3-ligase activity leads to dopaminergic neuronal degeneration in early-onset autosomal recessive juvenile parkinsonism, suggesting a key role of parkin for dopamine neuron survival. To evaluate the potential neuroprotective role of parkin in the pathogenesis of PD, we tested whether overexpression of wild-type rat parkin could protect against the toxicity of mutated human A30P alpha-synuclein in a rat lentiviral model of PD. Animals overexpressing parkin showed significant reductions in alpha-synuclein-induced neuropathology, including preservation of tyrosine hydroxylase-positive cell bodies in the substantia nigra and sparing of tyrosine hydroxylase-positive nerve terminals in the striatum. The parkin-mediated neuroprotection was associated with an increase in hyperphosphorylated alpha-synuclein inclusions, suggesting a key role for parkin in the genesis of Lewy bodies. These results indicate that parkin gene therapy may represent a promising candidate treatment for PD.
- Published
- 2004
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28. Circulating levels of soluble adhesion molecules in patients with ANCA-associated vasculitis.
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Di Lorenzo G, Pacor ML, Mansueto P, Lo Bianco C, Di Natale E, Rapisarda F, Pellitteri ME, Ditta V, Gioè A, Giammarresi G, Rini GB, and Li Vecchi M
- Subjects
- Adult, Aged, Case-Control Studies, Creatinine blood, E-Selectin chemistry, Female, Humans, Intercellular Adhesion Molecule-1 chemistry, Kidney Failure, Chronic blood, Kidney Failure, Chronic etiology, Kidney Failure, Chronic immunology, Male, Middle Aged, Osmolar Concentration, Peroxidase blood, Polycystic Kidney, Autosomal Dominant complications, Solubility, Vasculitis physiopathology, Vasculitis therapy, Antibodies, Antineutrophil Cytoplasmic blood, E-Selectin blood, Intercellular Adhesion Molecule-1 blood, Vasculitis blood, Vasculitis immunology
- Abstract
Background: During inflammation, activated vascular endothelial cells and other cell types express various adhesion molecules, which facilitate the binding of circulating leukocytes and their extravasation in surrounding tissue (i.e. renal tissue). The serum concentration of circulating soluble adhesion molecules is supposed to reflect the degree of this activation., Objective: In the first part of the study, we determined if the serum levels of the soluble intercellular adhesion molecule (sICAM)-1 and the soluble endothelial cell-leukocyte adhesion molecule (sELAM)-1, in patients affected by microscopic polyangiitis (MPA), associated with myeloperoxidase (MPO)-anti-neutrophil cytoplasmic antibodies (ANCA), were related to the active and the inactive vasculitis phase. In the second part of the study, we examined the changes in circulating sICAM-1 and sELAM-1 levels and the clinical outcome of renal function in these patients., Methods: We examined 20 MPO-ANCA-positive MPA patients in an acute phase and in a remission phase, after 6 months of treatment, and 50 subjects as controls, 30 with autosomal dominant polycystic kidney disease (ADPKD) in stable chronic renal failure (CRF) and 20 healthy volunteers (HS) with normal renal function., Results: Regarding serum creatinine (Cr) concentration, no significant differences were found comparing active and inactive phases in the MPA group and the CRF group. Mean serum adhesion molecule levels in the MPA group were higher in the active phase compared to the inactive phase and to the CRF and HS groups. In addition, considering the outcome of serum Cr concentrations in the MPA group, the serum adhesion molecule levels were higher and decreased more slowly in patients with final high serum Cr concentrations than in patients with final normal serum Cr concentrations., Conclusion: Our data suggest that in MPO-ANCA-positive MPA patients, higher sICAM-1 and sELAM-1 levels during the active phase and their slower decline during the treatment period, could be a prognostic risk factor for CRF development.
- Published
- 2004
29. alpha -Synucleinopathy and selective dopaminergic neuron loss in a rat lentiviral-based model of Parkinson's disease.
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Lo Bianco C, Ridet JL, Schneider BL, Deglon N, and Aebischer P
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- Animals, Animals, Genetically Modified, Biomarkers, Brain metabolism, Brain pathology, Disease Models, Animal, Gene Expression, Genetic Vectors, HIV-1, Humans, Lentivirus, Lewy Bodies metabolism, Lewy Bodies pathology, Nerve Degeneration metabolism, Nerve Tissue Proteins genetics, Nerve Tissue Proteins metabolism, Neurons metabolism, Parkinson Disease metabolism, Rats, Substantia Nigra metabolism, Synucleins, Tumor Cells, Cultured, Tyrosine 3-Monooxygenase metabolism, alpha-Synuclein, Dopamine, Nerve Degeneration pathology, Nerve Tissue Proteins physiology, Neurons pathology, Parkinson Disease pathology
- Abstract
Parkinson's disease (PD) is characterized by the progressive loss of substantia nigra dopaminergic neurons and the presence of cytoplasmic inclusions named Lewy bodies. Two missense mutations of the alpha-synuclein (alpha-syn; A30P and A53T) have been described in several families with an autosomal dominant form of PD. alpha-Syn also constitutes one of the main components of Lewy bodies in sporadic cases of PD. To develop an animal model of PD, lentiviral vectors expressing different human or rat forms of alpha-syn were injected into the substantia nigra of rats. In contrast to transgenic mice models, a selective loss of nigral dopaminergic neurons associated with a dopaminergic denervation of the striatum was observed in animals expressing either wild-type or mutant forms of human alpha-syn. This neuronal degeneration correlates with the appearance of abundant alpha-syn-positive inclusions and extensive neuritic pathology detected with both alpha-syn and silver staining. Lentiviral-mediated expression of wild-type or mutated forms of human alpha-syn recapitulates the essential neuropathological features of PD. Rat alpha-syn similarly leads to protein aggregation but without cell loss, suggesting that inclusions are not the primary cause of cell degeneration in PD. Viral-mediated genetic models may contribute to elucidate the mechanism of alpha-syn-induced cell death and allow the screening of candidate therapeutic molecules.
- Published
- 2002
- Full Text
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