Guo,Jiejie, Cao,Yanzi, Zhang,Ting, Xu,Chunshuang, Liu,Zhitao, Li,Wanyi, Wang,Qinwen, Guo,Jiejie, Cao,Yanzi, Zhang,Ting, Xu,Chunshuang, Liu,Zhitao, Li,Wanyi, and Wang,Qinwen
Jiejie Guo,1â 3 Yanzi Cao,1 Ting Zhang,2 Chunshuang Xu,1 Zhitao Liu,1,4 Wanyi Li,1 Qinwen Wang1 1Zhejiang Key Laboratory of Pathophysiology, NBU Health Science Center, Ningbo University, Ningbo, Peopleâs Republic of China; 2Department of Clinical Laboratory, The First Peopleâs Hospital of Wenling, Taizhou, Peopleâs Republic of China; 3Department of Neurology, The First Peopleâs Hospital of Wenling, Taizhou, Peopleâs Republic of China; 4Fujian Normal University, Fuzhou, Peopleâs Republic of ChinaCorrespondence: Qinwen Wang, Zhejiang Key Laboratory of Pathophysiology, NBU Health Science Center Ningbo University, Ningbo, Peopleâs Republic of China, Email wangqinwen@nbu.edu.cnBackground: There is growing interest in the role of physical activity in patients with of Alzheimerâs disease (AD), particularly regarding its impact of cognitive function, gut microbiota, metabolites, and neurotrophic factors.Objective: To investigate the impact of multisensory fusion training (MSFT) combined with 7, 8-dihydroxyflavone (DHF) on the behavioral characteristics, protein expression, microbiome, and serum metabolome using the AD model in mice induced with amyloid-β (Aβ).Methods: We assessed cognitive ability, anxiety-like and depression-like behaviors in Aβ mice using behavioral measures. Western blotting was employed to detect the expression of relevant proteins. The 16S rRNA gene sequencing and metabolomics were used to analyze changes in the intestinal microbial composition and serum metabolic profile, respectively, of Aβ mice.Results: The behavioral outcomes indicated that a 4-week intervention combining DHF and MSFT yielded remarkable improvements in cognitive function and reduced anxiety and depression-like behaviors in Aβ mice. In the hippocampus of Aβ mice, the combined intervention increased the levels of BDNF, VGF, PSD-95, Nrf2, p-GSK3β and p-CREB proteins. Analyses of sequence and metabolomic data revealed that Bacteroides and Ruminococcaceae were r