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This study investigated the effects of biset, drop-set and traditional resistance training (RT) techniques on metabolic responses in resistance-trained males. Fifteen trained males (age 29.7 ± 6.1 years; body mass 83.4 ± 7.6 kg; RT experience 11.4 ± 6.7 years; one-repetition maximum (1RM) barbell bench press: body mass ratio 1.4 ± 0.1 a.u.) were assigned to three experimental conditions, in a randomized crossover design. The experimental conditions were bi-set (3×10 repetitions at 70%1RM in barbell bench press followed by 10 repetitions at 60%1RM in incline bench press), drop-set (3×10 repetitions at 70%1RM followed by 10 repetitions at 50%1RM in barbell bench press) and traditional RT (3×20 at 60%1RM in barbell bench press). A portable gas analyzer was used to assess energy expenditure and maximal oxygen uptake during the experimental protocols. Blood lactate levels were assessed at baseline and 1, 3, and 5 minutes after the training session. There were no differences for total training volume ( p = 0.999). Post hoc comparisons revealed that bi-set elicited higher aerobic energy expenditure ( p = 0.003 vs. drop-set; p < 0.001 vs. traditional RT) and aerobic oxygen consumption ( p = 0.034 vs. drop-set; p < 0.001 vs. traditional RT) than other RT schemes. There were no differences regarding anaerobic EE between-conditions ( p > 0.05). There was a main effect of time and condition for blood lactate levels ( p < 0.001). Post hoc comparisons revealed that drop-set training elicited higher blood lactate levels than traditional RT ( p = 0.009). The results suggest that RT techniques may have a potential role in optimizing metabolic responses in resistance-trained males.