147 results on '"Lauber, Emmanuelle"'
Search Results
2. Genome‐wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection
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Luneau, Julien S, Baudin, Maël, Monnens, Thomas Quiroz, Carrère, Sébastien, Bouchez, Olivier, Jardinaud, Marie‐Françoise, Gris, Carine, François, Jonas, Ray, Jayashree, Torralba, Babil, Arlat, Matthieu, Lewis, Jennifer D, Lauber, Emmanuelle, Deutschbauer, Adam M, Noël, Laurent D, and Boulanger, Alice
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Infectious Diseases ,Genetics ,Prevention ,Emerging Infectious Diseases ,2.2 Factors relating to the physical environment ,Aetiology ,Infection ,Zero Hunger ,Bacterial Proteins ,Brassica ,Plant Diseases ,Virulence ,Xanthomonas campestris ,Xylem ,Brassica oleracea ,fitness ,hydathodes ,RB-TnSeq ,Xanthomonas ,xylem ,Brassica oleracea ,Xanthomonas ,Biological Sciences ,Agricultural and Veterinary Sciences ,Plant Biology & Botany - Abstract
Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
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- 2022
3. Bacterial host adaptation through sequence and structural variations of a single type III effector gene
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Lauber, Emmanuelle, González-Fuente, Manuel, Escouboué, Maxime, Vicédo, Céline, Luneau, Julien S., Pouzet, Cécile, Jauneau, Alain, Gris, Carine, Zhang, Zhi-Min, Pichereaux, Carole, Carrère, Sébastien, Deslandes, Laurent, and Noël, Laurent D.
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- 2024
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4. Arabidopsis thaliana Early Foliar Proteome Response to Root Exposure to the Rhizobacterium Pseudomonas simiae WCS417
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Marzorati, Francesca, primary, Rossi, Rossana, additional, Bernardo, Letizia, additional, Mauri, Pierluigi, additional, Silvestre, Dario Di, additional, Lauber, Emmanuelle, additional, Noël, Laurent D., additional, Murgia, Irene, additional, and Morandini, Piero, additional
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- 2023
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5. A Putative Ca 2+ and Calmodulin-Dependent Protein Kinase Required for Bacterial and Fungal Symbioses
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Lévy, Julien, Bres, Cécile, Geurts, René, Chalhoub, Boulos, Kulikova, Olga, Duc, Gérard, Journet, Etienne-Pascal, Ané, Jean-Michel, Lauber, Emmanuelle, Bisseling, Ton, Dénarié, Jean, Rosenberg, Charles, and Debellé, Frédéric
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- 2004
6. Immunity at Cauliflower Hydathodes Controls Systemic Infection by Xanthomonas campestris pv campestris
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Cerutti, Aude, Jauneau, Alain, Auriac, Marie-Christine, Lauber, Emmanuelle, Martinez, Yves, Chiarenza, Serge, Leonhardt, Nathalie, Berthomé, Richard, and Noël, Laurent D.
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- 2017
7. Cruciferous Weed Isolates of Xanthomonas campestris Yield Insight into Pathovar Genomic Relationships and Genetic Determinants of Host and Tissue Specificity
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Dubrow, Zoë E., primary, Carpenter, Sara C. D., additional, Carter, Morgan E., additional, Grinage, Ayress, additional, Gris, Carine, additional, Lauber, Emmanuelle, additional, Butchachas, Jules, additional, Jacobs, Jonathan M., additional, Smart, Christine D., additional, Tancos, Matthew A., additional, Noël, Laurent D., additional, and Bogdanove, Adam J., additional
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- 2022
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8. A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection
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Luneau, Julien, primary, Noël, Laurent, additional, Lauber, Emmanuelle, additional, and Boulanger, Alice, additional
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- 2022
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9. Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages
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Luneau, Julien S., primary, Cerutti, Aude, additional, Roux, Brice, additional, Carrère, Sébastien, additional, Jardinaud, Marie‐Françoise, additional, Gaillac, Antoine, additional, Gris, Carine, additional, Lauber, Emmanuelle, additional, Berthomé, Richard, additional, Arlat, Matthieu, additional, Boulanger, Alice, additional, and Noël, Laurent D., additional
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- 2021
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10. The Same against Many: AtCML8, a Ca2+ Sensor Acting as a Positive Regulator of Defense Responses against Several Plant Pathogens
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Zhu, Xiaoyang, Mazard, Julie, Robe, Eugénie, Pignoly, Sarah, Aguilar, Marielle, San Clemente, Hélène, Lauber, Emmanuelle, Berthomé, Richard, Galaud, Jean-Philippe, South China Agricultural University (SCAU), Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), the CaPPTure project, the International Science and Technology Cooperation Major Project Cultivation Special Fund of SCAU (2019SCAUGH05), Pearl River Talent Program for Young Talent (grant no. 2017GC010321)., ANR-17-CE20-0017,CaPPTure,Interaction plante-pathogène: influence de la température et contribution de la signalisation calcium(2017), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), and ANR-18-EURE-0019,TULIP-GSR,The Toulouse-Perpignan(2018)
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Arabidopsis thaliana ,QH301-705.5 ,Ralstonia solanacearum ,food and beverages ,Xanthomonas campestris ,calcium signaling ,[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity ,[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy ,Phytophtora capsica ,Chemistry ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,calmodulin-like protein ,Biology (General) ,plant immunity ,QD1-999 ,multi-pathogens - Abstract
Calcium signals are crucial for the activation and coordination of signaling cascades leading to the establishment of plant defense mechanisms. Here, we studied the contribution of CML8, an Arabidopsis calmodulin-like protein in response to Ralstonia solanacearum and to pathogens with different lifestyles, such as Xanthomonas campestris pv. campestris and Phytophtora capsici. We used pathogenic infection assays, gene expression, RNA-seq approaches, and comparative analysis of public data on CML8 knockdown and overexpressing Arabidopsis lines to demonstrate that CML8 contributes to defense mechanisms against pathogenic bacteria and oomycetes. CML8 gene expression is finely regulated at the root level and manipulated during infection with Ralstonia, and CML8 overexpression confers better plant tolerance. To understand the processes controlled by CML8, genes differentially expressed at the root level in the first hours of infection have been identified. Overexpression of CML8 also confers better tolerance against Xanthomonas and Phytophtora, and most of the genes differentially expressed in response to Ralstonia are differentially expressed in these different pathosystems. Collectively, CML8 acts as a positive regulator against Ralstonia solanaceraum and against other vascular or root pathogens, suggesting that CML8 is a multifunctional protein that regulates common downstream processes involved in the defense response of plants to several pathogens.
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- 2021
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11. Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptation at early infection stages
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Noël, Laurent D., Luneau, Julien, Cerutti, Aude, Roux, Brice, Carrère, Sébastien, Jardinaud, Marie-Françoise, Gaillac, Antoine, Gris, Carine, Lauber, Emmanuelle, Berthomé, Richard, Arlat, Matthieu, Boulanger, Alice, Noël, Laurent, Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), the COST action CA16107 EuroXanth., ANR-10-GENM-0013,XANTHOMIX,Etude comparative des génomes et des transcriptomes de Xanthomonas phytopathogènes(2010), ANR-10-JCJC-1703,XOPAQUE,Immunité vasculaire des plantes dépendante de XopAC(2010), ANR-18-CE20-0020,NEPHRON,Analyse génétique et moléculaire de l'immunité de l'hydathode et du système vasculaire(2018), ANR-19-CE20-0014,XBOX,L'étoffe d'un pathogène : comment Xanthomonas s'adapte à la vie in planta(2019), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), and ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011)
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Xanthomonas ,Soil Science ,Virulence ,Brassica ,adaptation ,Plant Science ,Xanthomonas campestris ,Microbiology ,Transcriptome ,03 medical and health sciences ,Bacterial Proteins ,type III secretion ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Molecular Biology ,Pathogen ,Gene ,Plant Diseases ,030304 developmental biology ,0303 health sciences ,biology ,030306 microbiology ,hrpG ,Gene Expression Regulation, Bacterial ,Original Articles ,hrp gene cluster ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,biology.organism_classification ,Adaptation, Physiological ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Hydathode ,hydathode ,Regulon ,Original Article ,Agronomy and Crop Science ,type III effector - Abstract
Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological and virulence strategies deployed by Xcc during early stages of infection, the transcriptomic profile of Xcc was analysed 3 days after entry into cauliflower hydathodes. Despite the absence of visible plant tissue alterations and despite a biotrophic lifestyle, 18% of Xcc genes were differentially expressed, including a striking repression of chemotaxis and motility functions. The Xcc full repertoire of virulence factors had not yet been activated but the expression of the HrpG regulon composed of 95 genes, including genes coding for the type III secretion machinery important for suppression of plant immunity, was induced. The expression of genes involved in metabolic adaptations such as catabolism of plant compounds, transport functions, sulphur and phosphate metabolism was upregulated while limited stress responses were observed 3 days postinfection. We confirmed experimentally that high‐affinity phosphate transport is needed for bacterial fitness inside hydathodes. This analysis provides information about the nutritional and stress status of bacteria during the early biotrophic infection stages and helps to decipher the adaptive strategy of Xcc to the hydathode environment., In planta transcriptomic analysis of Xanthomonas campestris inside cauliflower hydathodes reveals the adaptative processes at play during early infection.
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- 2021
12. Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris
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Boulanger, Alice, Dejean, Guillaume, Lautier, Martine, Glories, Marie, Zischek, Claudine, Arlat, Matthieu, and Lauber, Emmanuelle
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Glucosamine -- Genetic aspects ,Glucosamine -- Research ,Quantitative trait loci -- Research ,Gene expression -- Research ,Biological sciences - Abstract
Xanthomonas campestris pv. campestris, the causal agent of black rot disease of brassicas, is known for its ability to catabolize a wide range of plant compounds. This ability is correlated with the presence of specific carbohydrate utilization loci containing TonB-dependent transporters (CUT loci) devoted to scavenging specific carbohydrates. In this study, we demonstrate that there is an X. campestris pv. campestris CUT system involved in the import and catabolism of N-acetylglucosamine (GlcNAc). Expression of genes belonging to this GIcNAc CUT system is under the control of GlcNAc via the LacI family NagR and GntR family NagQ regulators. Analysis of the NagR and NagQ regulons confirmed that GlcNAc utilization involves NagA and NagB-II enzymes responsible for the conversion of GlcNAc-6-phosphate to fructose-6-phosphate. Mutants with mutations in the corresponding genes are sensitive to GlcNAc, as previously reported for Escherichia coli. This GlcNAc sensitivity and analysis of the NagQ and NagR regulons were used to dissect the X. campestris pv. campestris GIcNAc utilization pathway. This analysis revealed specific features, including the fact that uptake of GlcNAc through the inner membrane occurs via a major facilitator superfamily transporter and the fact that this amino sugar is phosphorylated by two proteins belonging to the glucokinase family, NagK-IIA and NagK-IIB. However, NagK-IIA seems to play a more important role in GlcNAc utilization than NagK-IIB under our experimental conditions. The X. campestris pv. campestris GlcNAc NagR regulon includes four genes encoding TonB-dependent active transporters (TBDTs). However, the results of transport experiments suggest that GlcNAc passively diffuses through the bacterial envelope, an observation that calls into question whether GlcNAc is a natural substrate for these TBDTs and consequently is the source of GlcNAc for this nonchitinolytic plant-associated bacterium. doi: 10.1128/JB.01418-09
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- 2010
13. Transcriptional reprogramming and phenotypical changes associated with growth of Xanthomonas campestris pv. campestris in cabbage xylem sap
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Dugé de Bernonville, Thomas, Noël, Laurent D., SanCristobal, Magali, Danoun, Saida, Becker, Anke, Soreau, Paul, Arlat, Matthieu, and Lauber, Emmanuelle
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- 2014
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14. [AvrAC.sub.Xcc8004], a type III effector with a leucine-rich repeat domain from Xanthomonas campestris pathovar campestris confers avirulence in vascular tissues of Arabidopsis thaliana ecotype Col-0
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Xu, Rong-Qi, Blanvillain, Servane, Feng, Jia-Xun, Jiang, Bo-Le, Li, Xian-Zhen, Wei, Hong-Yu, Kroj, Thomas, Lauber, Emmanuelle, Roby, Dominique, Chen, Baoshan, He, Yong-Qiang, Lu, Guang-Tao, Tang, Dong-Jie, Vasse, Jacques, Arlat, Matthieu, and Tang, Ji-Liang
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Arabidopsis thaliana -- Genetic aspects ,Arabidopsis thaliana -- Health aspects ,Virulence (Microbiology) -- Research ,Xanthoma -- Research ,Bacterial proteins -- Health aspects ,Biological sciences - Abstract
Xanthomonas campestris pathovar campestris causes black rot, a vascular disease on cruciferous plants, including Arabidopsis thaliana. The gene XC1553 from X. campestris pv. campestris strain 8004 encodes a protein containing leucine-rich repeats (LRRs) and appears to be restricted to strains of X. campestris pv. campestris. LRRs are found in a number of type III-secreted effectors in plant and animal pathogens. These prompted us to investigate the role of the XC1553 gene in the interaction between X. campestris pv. campestris and A. thaliana. Translocation assays using the hypersensitive-reaction-inducing domain of X. campestris pv. campestris AvrBs1 as a reporter revealed that XC1553 is a type III effector. Infiltration of Arabidopsis leaf mesophyll with bacterial suspensions showed no differences between the wild-type strain and an XC1553 gene mutant: both strains induced disease symptoms on Kashmir and Col-0 ecotypes. However, a clear difference was observed when bacteria were introduced into the vascular system by piercing the central vein of leaves. In this case, the wild-type strain 8004 caused disease on the Kashmir ecotype, but not on ecotype Col-0; the XC1553 gene mutant became virulent on the Col-0 ecotype and still induced disease on the Kashmir ecotype. Altogether, these data show that the XC1553 gene, which was renamed [avrAC.sub.Xcc8004], functions as an avirulence gene whose product seems to be recognized in vascular tissues.
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- 2008
15. PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family
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Meyer, Damien, Cunnac, Sebastien, Gueneron, Mareva, Declercq, Celine, Van Gijsegem, Frederique, Lauber, Emmanuelle, Boucher, Christian, and Arlat, Matthieu
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Bacterial proteins -- Research ,Bacteria, Pathogenic -- Genetic aspects ,Bacteria, Pathogenic -- Physiological aspects ,Translocation (Genetics) -- Research ,Biological sciences - Abstract
Ralstonia solanacearum GMI1000 is a gram-negative plant pathogen which contains an hrp gene cluster which codes for a type III protein secretion system (TTSS). We identified two novel Hrp-secreted proteins, called PopF1 and PopF2, which display similarity to one another and to putative TTSS translocators, HrpF and NopX, from Xanthomonas spp. and rhizobia, respectively. They also show similarities with TTSS translocators of the YopB family from animal-pathogenic bacteria. Both popF1 and popF2 belong to the HrpB regulon and are required for the interaction with plants, but PopFl seems to play a more important role in virulence and hypersensitive response (HR) elicitation than PopF2 under our experimental conditions. PopF1 and PopF2 are not necessary for the secretion of effector proteins, but they are required for the translocation of AvrA avirulence protein into tobacco cells. We conclude that PopFl and PopF2 are type III translocators belonging to the HrpF/NopX family. The hrpF gene of Xanthomonas campestris pv. campestris partially restored HR-inducing ability to popF1 popF2 mutants of R. solanacearum, suggesting that translocators of R. solanacearum and Xanthomonas are functionally conserved. Finally, R. solanacearum strain UW551, which does not belong to the same phylotype as GMI1000, also possesses two putative translocator proteins. However, although one of these proteins is clearly related to PopFl and PopF2, the other seems to be different and related to NopX proteins, thus showing that translocators might be variable in R. solanacearum.
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- 2006
16. From effectors to effectomes: Are functional studies of individual effectors enough to decipher plant pathogen infectious strategies?
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Arroyo-Velez, Noe, primary, González-Fuente, Manuel, additional, Peeters, Nemo, additional, Lauber, Emmanuelle, additional, and Noël, Laurent D., additional
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- 2020
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17. A putative [Ca.sup.2+] and calmodulin-dependent protein kinase required for bacterial and fungal symbioses
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Levy, Julien, Bres, Cecile, Geurts, Rene, Chalhoub, Boulos, Kulikova, Olga, Duc, Gerard, Journet, Etienne-Pascal, Ane, Jean-Michel, Lauber, Emmanuelle, Bisseling, Ton, Denarie, Jean, Rosenberg, Charles, and Debelle, Frederic
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Alfalfa -- Genetic aspects -- Chemical properties ,Symbiosis -- Chemical properties -- Genetic aspects ,Mycorrhizas -- Genetic aspects -- Chemical properties ,Science and technology ,Chemical properties ,Genetic aspects - Abstract
Legumes can enter into symbiotic relationships with both nitrogen-fixing bacteria (rhizobia) and mycorrhizal fungi. Nodulation by rhizobia results from a signal transduction pathway induced in legume roots by rhizobial Nod factors. DMI3, a Medicago truncatula gene that acts immediately downstream of calcium spiking in this signaling pathway and is required for both nodulation and mycorrhizal infection, has high sequence similarity to genes encoding calcium and calmodulin-dependent protein kinases (CCaMKs). This indicates that calcium spiking is likely an essential component of the signaling cascade leading to nodule development and mycorrhizal infection, and sheds light on the biological role of plant CCaMKs., The legume-rhizobia symbiosis fixes as much nitrogen worldwide as the chemical fertilizer industry, owing to the ability of rhizobial bacteria to induce the morphogenesis of a new plant organ, the [...]
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- 2004
18. Xanthomonas transcriptome inside cauliflower hydathodes reveals bacterial virulence strategies and physiological adaptations at early infection stages.
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Luneau, Julien S., Cerutti, Aude, Roux, Brice, Carrère, Sébastien, Jardinaud, Marie‐Françoise, Gaillac, Antoine, Gris, Carine, Lauber, Emmanuelle, Berthomé, Richard, Arlat, Matthieu, Boulanger, Alice, and Noël, Laurent D.
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PHYSIOLOGICAL adaptation ,XANTHOMONAS ,CAULIFLOWER ,XANTHOMONAS campestris ,TRANSCRIPTOMES - Abstract
Xanthomonas campestris pv. campestris (Xcc) is a seed‐transmitted vascular pathogen causing black rot disease on cultivated and wild Brassicaceae. Xcc enters the plant tissues preferentially via hydathodes, which are organs localized at leaf margins. To decipher both physiological and virulence strategies deployed by Xcc during early stages of infection, the transcriptomic profile of Xcc was analysed 3 days after entry into cauliflower hydathodes. Despite the absence of visible plant tissue alterations and despite a biotrophic lifestyle, 18% of Xcc genes were differentially expressed, including a striking repression of chemotaxis and motility functions. The Xcc full repertoire of virulence factors had not yet been activated but the expression of the HrpG regulon composed of 95 genes, including genes coding for the type III secretion machinery important for suppression of plant immunity, was induced. The expression of genes involved in metabolic adaptations such as catabolism of plant compounds, transport functions, sulphur and phosphate metabolism was upregulated while limited stress responses were observed 3 days postinfection. We confirmed experimentally that high‐affinity phosphate transport is needed for bacterial fitness inside hydathodes. This analysis provides information about the nutritional and stress status of bacteria during the early biotrophic infection stages and helps to decipher the adaptive strategy of Xcc to the hydathode environment. [ABSTRACT FROM AUTHOR]
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- 2022
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19. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels
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Pieretti Isabelle, Royer Monique, Barbe Valérie, Carrere Sébastien, Koebnik Ralf, Couloux Arnaud, Darrasse Armelle, Gouzy Jérôme, Jacques Marie-Agnès, Lauber Emmanuelle, Manceau Charles, Mangenot Sophie, Poussier Stéphane, Segurens Béatrice, Szurek Boris, Verdier Valérie, Arlat Matthieu, Gabriel Dean W, Rott Philippe, and Cociancich Stéphane
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of Xanthomonas. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic X. albilineans strain GPE PC73 focused exclusively on distinctive genomic features shared with Xylella fastidiosa—another xylem-limited Xanthomonadaceae. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of X. albilineans, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity. Results Comparative genomic analyses showed that most of the known pathogenicity factors from other Xanthomonas species are conserved in X. albilineans, with the notable absence of two major determinants of the “artillery” of other plant pathogenic species of Xanthomonas: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to X. albilineans that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to X. albilineans were identified, supposing that X. albilineans has a reduced artillery compared to other pathogenic Xanthomonas species. Particular attention has therefore been given to genomic features specific to X. albilineans making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems. Conclusions This study confirms that X. albilineans is a highly distinctive species within the genus Xanthomonas, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen.
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- 2012
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20. The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae
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Szurek Boris, Segurens Béatrice, Poussier Stéphane, Manceau Charles, Mangenot Sophie, Lauber Emmanuelle, Jacques Marie-Agnès, Darrasse Armelle, Gouzy Jérôme, Couloux Arnaud, Cociancich Stéphane, Koebnik Ralf, Carrere Sébastien, Barbe Valérie, Royer Monique, Pieretti Isabelle, Verdier Valérie, Arlat Matthieu, and Rott Philippe
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The Xanthomonadaceae family contains two xylem-limited plant pathogenic bacterial species, Xanthomonas albilineans and Xylella fastidiosa. X. fastidiosa was the first completely sequenced plant pathogen. It is insect-vectored, has a reduced genome and does not possess hrp genes which encode a Type III secretion system found in most plant pathogenic bacteria. X. fastidiosa was excluded from the Xanthomonas group based on phylogenetic analyses with rRNA sequences. Results The complete genome of X. albilineans was sequenced and annotated. X. albilineans, which is not known to be insect-vectored, also has a reduced genome and does not possess hrp genes. Phylogenetic analysis using X. albilineans genomic sequences showed that X. fastidiosa belongs to the Xanthomonas group. Order of divergence of the Xanthomonadaceae revealed that X. albilineans and X. fastidiosa experienced a convergent reductive genome evolution during their descent from the progenitor of the Xanthomonas genus. Reductive genome evolutions of the two xylem-limited Xanthomonadaceae were compared in light of their genome characteristics and those of obligate animal symbionts and pathogens. Conclusion The two xylem-limited Xanthomonadaceae, during their descent from a common ancestral parent, experienced a convergent reductive genome evolution. Adaptation to the nutrient-poor xylem elements and to the cloistered environmental niche of xylem vessels probably favoured this convergent evolution. However, genome characteristics of X. albilineans differ from those of X. fastidiosa and obligate animal symbionts and pathogens, indicating that a distinctive process was responsible for the reductive genome evolution in this pathogen. The possible role in genome reduction of the unique toxin albicidin, produced by X. albilineans, is discussed.
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- 2009
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21. Two ancestral genes shaped theXanthomonas campestrisTALeffector gene repertoire
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Denancé, Nicolas, primary, Szurek, Boris, additional, Doyle, Erin L., additional, Lauber, Emmanuelle, additional, Fontaine‐Bodin, Lisa, additional, Carrère, Sébastien, additional, Guy, Endrick, additional, Hajri, Ahmed, additional, Cerutti, Aude, additional, Boureau, Tristan, additional, Poussier, Stéphane, additional, Arlat, Matthieu, additional, Bogdanove, Adam J., additional, and Noël, Laurent D., additional
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- 2018
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22. The N-Glycan Cluster from Xanthomonas campestris pv. campestris: A toolbox for sequential plant n-glycan processing
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Dupoiron, Stéphanie, Zischek, Claudine, Ligat, Laetitia, Carbonne, Julien, Boulanger, Alice, Duge De Bernonville, Thomas, Lautier, Martine, RIVAL, Pauline, Arlat, Matthieu, Jamet, Elisabeth, Lauber, Emmanuelle, Albenne, Cécile, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Interactions Microbiennes dans la Rhizosphère et les Racines, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), and Dynamique et Evolution des Parois cellulaires végétales
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Enzyme Kinetics ,Xanthomonas ,Glycoside Hydrolases ,Bacteria ,N-Linked Glycosylation ,[SDV]Life Sciences [q-bio] ,Glycoside Hydrolase ,food and beverages ,Brassica ,Plant ,Xanthomonas campestris ,Carbohydrate Processing ,Phytopathogen ,Xylosidases ,Polysaccharides ,alpha-Mannosidase ,Enzymology ,bacteria ,Humans ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,hormones, hormone substitutes, and hormone antagonists ,Plant Diseases - Abstract
International audience; N-Glycans are widely distributed in living organisms but represent only a small fraction of the carbohydrates found in plants. This probably explains why they have not previously been considered as substrates exploited by phytopathogenic bacteria during plant infection. Xanthomonas campestris pv. campestris, the causal agent of black rot disease of Brassica plants, possesses a specific system for GlcNAc utilization expressed during host plant infection. This system encompasses a cluster of eight genes (nixE to nixL) encoding glycoside hydrolases (GHs). In this paper, we have characterized the enzymatic activities of these GHs and demonstrated their involvement in sequential degradation of a plant N-glycan using a N-glycopeptide containing two GlcNAcs, three mannoses, one fucose, and one xylose (N2M3FX) as a substrate. The removal of the α-1,3-mannose by the α-mannosidase NixK (GH92) is a prerequisite for the subsequent action of the β-xylosidase NixI (GH3), which is involved in the cleavage of the β-1,2-xylose, followed by the α-mannosidase NixJ (GH125), which removes the α-1,6-mannose. These data, combined to the subcellular localization of the enzymes, allowed us to propose a model of N-glycopeptide processing by X. campestris pv. campestris. This study constitutes the first evidence suggesting N-glycan degradation by a plant pathogen, a feature shared with human pathogenic bacteria. Plant N-glycans should therefore be included in the repertoire of molecules putatively metabolized by phytopathogenic bacteria during their life cycle.
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- 2015
23. Additional file 5: of Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome
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Roux, Brice, Bolot, Stéphanie, Endrick Guy, Denancé, Nicolas, Lautier, Martine, Marie-Françoise Jardinaud, Saux, Marion Fischer-Le, Portier, Perrine, Marie-Agnès Jacques, Gagnevin, Lionel, Pruvost, Olivier, Lauber, Emmanuelle, Arlat, Matthieu, Carrère, Sébastien, Koebnik, Ralf, and Noël, Laurent
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body regions ,nervous system ,fungi - Abstract
Oligonucleotides used in this study. (PDF 865 kb)
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- 2015
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24. Additional file 2: of Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome
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Roux, Brice, Bolot, Stéphanie, Endrick Guy, Denancé, Nicolas, Lautier, Martine, Marie-Françoise Jardinaud, Saux, Marion Fischer-Le, Portier, Perrine, Marie-Agnès Jacques, Gagnevin, Lionel, Pruvost, Olivier, Lauber, Emmanuelle, Arlat, Matthieu, Carrère, Sébastien, Koebnik, Ralf, and Noël, Laurent
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Scatter plots of the normalized expression levels of the 141 hrpG -regulated genes of strain CFBP 5828R of X. campestris pv. raphani by RNA sequencing (Table 4 , Fig. 4a ). (PDF 635 kb)
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- 2015
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25. Development of Sinorhizobium meliloti pilot macroarrays for transcriptome analysis
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Berges, Helene, Lauber, Emmanuelle, Liebe, Carine, Batut, Jacques, Kahn, Daniel, de Bruijn, Frans J., and Ampe, Frederic
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Host-bacteria relationships -- Genetic aspects ,Host-bacteria relationships -- Analysis ,Genomes -- Analysis ,Gene expression -- Analysis ,DNA -- Analysis ,Biological sciences - Abstract
Research describes the designing of DNA macroarrays of Sinorhizobium meliloti genes and testing them in the bacteium's gene expression process. Thirtyfour regulatory genes are used in the construction of macroarrays to assess the length of the polymerase chain reaction products, the influence of of the 5' tag of the primers, and RNA labeling method.
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- 2003
26. Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome
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Roux, Brice, Bolot, Stéphanie, Guy, Endrick, Denancé, Nicolas, Lautier, Martine, Jardinaud, Marie-Françoise, Fischer-Le-Caux, Marion, Portier, Perrine, Jacques, Marie Agnès, Gagnevin, Lionel, Pruvost, Olivier, Lauber, Emmanuelle, Arlat, Mathieu, Carrère, Sébastien, Koebnik, Ralf, Noel, Laurent D., Roux, Brice, Bolot, Stéphanie, Guy, Endrick, Denancé, Nicolas, Lautier, Martine, Jardinaud, Marie-Françoise, Fischer-Le-Caux, Marion, Portier, Perrine, Jacques, Marie Agnès, Gagnevin, Lionel, Pruvost, Olivier, Lauber, Emmanuelle, Arlat, Mathieu, Carrère, Sébastien, Koebnik, Ralf, and Noel, Laurent D.
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Background The bacterial species Xanthomonas campestris infects a wide range of Brassicaceae. Specific pathovars of this species cause black rot (pv. campestris), bacterial blight of stock (pv. incanae) or bacterial leaf spot (pv. raphani). Results In this study, we extended the genomic coverage of the species by sequencing and annotating the genomes of strains from pathovar incanae (CFBP 1606R and CFBP 2527R), pathovar raphani (CFBP 5828R) and a pathovar formerly named barbareae (CFBP 5825R). While comparative analyses identified a large core ORFeome at the species level, the core type III effectome was limited to only three putative type III effectors (XopP, XopF1 and XopAL1). In Xanthomonas, these effector proteins are injected inside the plant cells by the type III secretion system and contribute collectively to virulence. A deep and strand-specific RNA sequencing strategy was adopted in order to experimentally refine genome annotation for strain CFBP 5828R. This approach also allowed the experimental definition of novel ORFs and non-coding RNA transcripts. Using a constitutively active allele of hrpG, a master regulator of the type III secretion system, a HrpG-dependent regulon of 141 genes co-regulated with the type III secretion system was identified. Importantly, all these genes but seven are positively regulated by HrpG and 56 of those encode components of the Hrp type III secretion system and putative effector proteins. Conclusions This dataset is an important resource to mine for novel type III effector proteins as well as for bacterial genes which could contribute to pathogenicity of X. campestris. (Résumé d'auteur)
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- 2015
27. Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome
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Roux, Brice, primary, Bolot, Stéphanie, additional, Guy, Endrick, additional, Denancé, Nicolas, additional, Lautier, Martine, additional, Jardinaud, Marie-Françoise, additional, Fischer-Le Saux, Marion, additional, Portier, Perrine, additional, Jacques, Marie-Agnès, additional, Gagnevin, Lionel, additional, Pruvost, Olivier, additional, Lauber, Emmanuelle, additional, Arlat, Matthieu, additional, Carrère, Sébastien, additional, Koebnik, Ralf, additional, and Noël, Laurent D., additional
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- 2015
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28. The xylan utilization system of the plant pathogen Xanthomonas campestris pv campestris controls epiphytic life and reveals common features with oligotrophic bacteria and animal gut symbionts
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Dejean, Guillaume, Blanvillain-Baufume, Servane, Boulanger, Alice, Darrasse, Armelle, Duge De Bernonville, Thomas, Girard, Anne-Laure, Carrere, Sebastien, jamet, Stevie, Zischek, Claudine, Lautier, Martine, Sole, Magali, Buettner, Daniela, Jacques, Marie Agnes, Lauber, Emmanuelle, Arlat, Matthieu, Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), LIPM, UMR441, Institut National de la Recherche Agronomique (INRA), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Departement Sante des Plantes et Environnement-Institut National de la Recherche Agronomique [2007_0441_02], French Agence Nationale de la Recherche [ANR-08-BAN-0193-01], Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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xylanase ,animal structures ,[SDV]Life Sciences [q-bio] ,BIOFILM FORMATION ,D-XYLOSE METABOLISM ,food and beverages ,ERWINIA-CHRYSANTHEMI ,PREVOTELLA-BRYANTII ,xylan ,UTILIZATION GENE-CLUSTER ,FUNCTIONAL-CHARACTERIZATION ,epiphytic ,ESCHERICHIA-COLI ,transport ,CAULOBACTER-CRESCENTUS ,bacteria ,TonB-dependent transporter ,gut symbiont ,GRAM-NEGATIVE BACTERIA ,oligotrophy ,DEPENDENT TRANSPORT - Abstract
Xylan is a major structural component of plant cell wall and the second most abundant plant polysaccharide in nature. Here, by combining genomic and functional analyses, we provide a comprehensive picture of xylan utilization by Xanthomonas campestris pv campestris (Xcc) and highlight its role in the adaptation of this epiphytic phytopathogen to the phyllosphere. The xylanolytic activity of Xcc depends on xylan-deconstruction enzymes but also on transporters, including two TonB-dependent outer membrane transporters (TBDTs) which belong to operons necessary for efficient growth in the presence of xylo-oligosaccharides and for optimal survival on plant leaves. Genes of this xylan utilization system are specifically induced by xylo-oligosaccharides and repressed by a LacI-family regulator named XylR. Part of the xylanolytic machinery of Xcc, including TBDT genes, displays a high degree of conservation with the xylose-regulon of the oligotrophic aquatic bacterium Caulobacter crescentus. Moreover, it shares common features, including the presence of TBDTs, with the xylan utilization systems of Bacteroides ovatus and Prevotella bryantii, two gut symbionts. These similarities and our results support an important role for TBDTs and xylan utilization systems for bacterial adaptation in the phyllosphere, oligotrophic environments and animal guts.
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- 2013
29. Two ancestral genes shaped the Xanthomonas campestris TAL effector gene repertoire.
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Denancé, Nicolas, Szurek, Boris, Doyle, Erin L., Lauber, Emmanuelle, Fontaine‐Bodin, Lisa, Carrère, Sébastien, Guy, Endrick, Hajri, Ahmed, Cerutti, Aude, Boureau, Tristan, Poussier, Stéphane, Arlat, Matthieu, Bogdanove, Adam J., and Noël, Laurent D.
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NUCLEOTIDE sequence ,NUCLEOTIDE sequencing ,XANTHOMONAS campestris ,GENE expression ,PLANT cells & tissues ,PHYSIOLOGY - Abstract
Summary: Xanthomonas transcription activator‐like effectors (TALEs) are injected inside plant cells to promote host susceptibility by enhancing transcription of host susceptibility genes. TALE‐encoding (tal) genes were thought to be absent from Brassicaceae‐infecting Xanthomonas campestris (Xc) genomes based on four reference genomic sequences. We discovered tal genes in 26 of 49 Xc strains isolated worldwide and used a combination of single molecule real time (SMRT) and tal amplicon sequencing to yield a near‐complete description of the TALEs found in Xc (Xc TALome). The 53 sequenced tal genes encode 21 distinct DNA binding domains that sort into seven major DNA binding specificities. In silico analysis of the Brassica rapa promoterome identified a repertoire of predicted TALE targets, five of which were experimentally validated using quantitative reverse transcription polymerase chain reaction. The Xc TALome shows multiple signs of DNA rearrangements that probably drove its evolution from two ancestral tal genes. We discovered that Tal12a and Tal15a of Xcc strain Xca5 contribute together in the development of disease symptoms on susceptible B. oleracea var. botrytis cv Clovis. This large and polymorphic repertoire of TALEs opens novel perspectives for elucidating TALE‐mediated susceptibility of Brassicaceae to black rot disease and for understanding the molecular processes underlying TALE evolution. [ABSTRACT FROM AUTHOR]
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- 2018
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30. Genome sequencing of Xanthomonas axonopodis pv. phaseoli CFBP4834-R reveals that flagellar motility is not a general feature of xanthomonads
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Darrasse, Armelle, Carrère, Sébastien, Barbe, Valérie, Bourreau, Tristan, Bernal, Adriana, Bonneau, Sophie, Brin, Christelle, Cociancich, Stéphane, Durand, Karine, Fouteau, Stéphanie, Gagnevin, Lionel, Gouzy, Jérôme, Guérin, Fabien, Guy, Endrick, Indiana, Arnaud, Koebnik, Ralf, Lauber, Emmanuelle, Munoz, Alejandra, Noel, Laurent D., Pieretti, Isabelle, Poussier, Stéphane, Pruvost, Olivier, Robène-Soustrade, Isabelle, Rott, Philippe, Royer, Monique, Szurek, Boris, Van Sluys, Marie-Anne, Verdier, Valérie, Vernière, Christian, Arlat, Mathieu, Manceau, Charles, and Jacques, Marie Agnès
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H20 - Maladies des plantes - Abstract
Xanthomonads are plant-associated bacteria that establish neutral, commensal or pathogenic relationships with plants. The list of common characteristics shared by all members of the genus Xanthomonas is now well established based on the entire genome sequences that are currently available and that represent various species, numerous pathovars of X. axonopodis (sensu Vauterin et al., 2000), X. oryzae and X. campestris, and many strains within some pathovars. These ?-proteobacteria are motile by a single polar flagellum. Motility is an important feature involved in biofilm formation, plant colonization and hence considered as a pathogenicity factor. X. axonopodis pv. phaseoli var. fuscans (Xapf) is one of the causal agents of common bacterial blight of bean and 4834-R is a highly aggressive strain of this pathogen that was isolated from a seed-borne epidemic in France in 1998. We obtained a high quality assembled sequence of the genome of this strain with 454-Solexa and 2X Sanger sequencing. Housekeeping functions are conserved in this genome that shares core characteristics with genomes of other xanthomonads: the six secretion systems which have been described so far in Gram negative bacteria are all present, as well as their ubiquitous substrates or effectors and a rather usual number of mobile elements. Elements devoted to the adaptation to the environment constitute an important part of the genome with a chemotaxis island and dispersed MCPs, numerous two-component systems, and numerous TonB dependent transporters. Furthermore, numerous multidrug efflux systems and functions dedicated to biofilm formation that confer resistance to stresses are also present. An intriguing feature revealed by genome analysis is a long deletion of 35 genes (33 kbp) involved in flagellar biosynthesis. This deletion is replaced by an insertion sequence called ISXapf2. Genes such as flgB to flgL and fliC to fleQ which are involved in the flagellar structure (rod, P- and L-ring, hook, cap and filament) are absent in the genome of strain 4834-R that is not motile. Primers were designed to detect this deletion by PCR in a collection of more than 300 strains representing different species and pathovars of Xanthomonas, and less than 5% of the tested xanthomonads strains were found nonmotile because of a deletion in the flagellum gene cluster. We observed that half of the Xapf strains isolated from the same epidemic than strain 4834-R was non-motile and that this ratio was conserved in the strains colonizing the next bean seed generation. Isolation of such variants in a natural epidemic reveals that either flagellar motility is not a key function for fitness or that some complementation occurs within the bacterial population.
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- 2012
31. The complete genome sequence of Xanthomonas albilineans provides insights into pathogenicity of this sugarcane pathogen and allows further assessments of the large diversity within this species
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Pieretti, Isabelle, Cociancich, Stéphane, Barbe, Valerie, Carrere, Sébastien, Koebnik, Ralf, Champoiseau, P., Couloux, Arnaud, Darrasse, Armelle, Gouzy, Jerome, Jacques, Marie-Agnès, Lauber, Emmanuelle, Manceau, Charles, Mangenot, Sophie, Marguerettaz, Mélanie, Poussier, Stéphane, Segurens, Béatrice, Szurek, Boris, Verdier, Valérie, Arlat, Mathieu, Gabriel, Dean W., Rott, Philippe, Royer, Monique, Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des interactions plantes micro-organismes (LIPM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD), Pathologie Végétale (PaVé), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Centre IRD de Montpellier, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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Xylella fastidiosa ,Saccharum officinarum ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Xanthomonas albilineans ,ComputingMilieux_MISCELLANEOUS ,H20 - Maladies des plantes - Abstract
Objectives: Xanthomonas albilineans is a xylem-invading pathogen that causes leaf scald, one of the major diseases of sugarcane. Previous studies revealed that i/ two important genomic features differentiate X. albilineans from other sequenced species of Xanthomonas: genome erosion and the presence of a type III secretion system (T3SS) of the SPI-1 family (1-2), and ii/ large variability exists among strains of X. albilineans and all strains involved in outbreaks of sugarcane leaf scald disease since the late 1980s belong to the same genetic group called PFGE-B (3). In the present study, we used the genome sequence of X. albilineans strain GPE PC73 to describe all pathogenicity-related features either shared with all species of Xanthomonas or specific to X. albilineans, and to further investigate the large diversity of this species. Results Among the major virulence factors described so far in Xanthomonads, most of them are conserved in X. albilineans, except the T3SS of the Hrp family and the gum gene clusters, and Hrp T3SS effectors. The genome of X. albilineans also encodes specific pathogenicity-related factors including twelve non ribosomal peptide synthetases and five enzymes harboring a specific cellulose binding domain. Several DNA fragments present in PFGE-B strains and absent in other strains of the pathogen were isolated by suppression subtractive hybridization (SSH). Additionally, occurrence of methylation of genomic DNA by a specific Dam methyltransferase in PFGE-B strains, but not in other strains, was experimentally demonstrated. Large inter-strain variability in X. albilineans was confirmed using multi locus sequence analysis (MLSA), clustered regularly interspaced short palindromic repeats (CRISPR) and SSH markers. Conclusions This study allowed us to identify several new candidate pathogenicity genes. In-depth functional analyses are now necessary to explore the role of these genes in the successful invasion of the sugarcane xylem vessels by X. albilineans.
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- 2011
32. Plant carbohydrate scavenging through TonB-dependent receptors by the phytopathogenic bacterium Xanthomonas campestris pv. Campestris
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Blanvillain, Servane, Meyer, Damien, Dejean, Guillaume, Boulanger, Alice, Lautier, Martine, Guynet, Catherine, Denancé, Nicolas, Vasse, Jacques, Lauber, Emmanuelle, and Arlat, Mathieu
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Pouvoir pathogène ,Pathologie végétale ,Relation hôte pathogène ,Facteur du milieu ,Xanthomonas campestris ,F60 - Physiologie et biochimie végétales ,Maladie des plantes ,Expression des gènes ,Glucide ,Adaptation ,H20 - Maladies des plantes - Published
- 2008
33. The Plant Pathogen Xanthomonas campestris pv. campestris Exploits N -Acetylglucosamine during Infection
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Boulanger, Alice, primary, Zischek, Claudine, additional, Lautier, Martine, additional, Jamet, Stevie, additional, Rival, Pauline, additional, Carrère, Sébastien, additional, Arlat, Matthieu, additional, and Lauber, Emmanuelle, additional
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- 2014
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34. Transcriptional reprogramming and phenotypical changes associated with growth ofXanthomonas campestrispv.campestrisin cabbage xylem sap
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Dugé de Bernonville, Thomas, primary, Noël, Laurent D., additional, SanCristobal, Magali, additional, Danoun, Saida, additional, Becker, Anke, additional, Soreau, Paul, additional, Arlat, Matthieu, additional, and Lauber, Emmanuelle, additional
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- 2014
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35. Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads
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Darrasse, Armelle, Carrère, Sébastien, Barbe, Valérie, Boureau, Tristan, Arrieta-Ortiz, Mario L., Bonneau, Sophie, Briand, Martial, Brin, Christelle, Cociancich, Stéphane, Durand, Karine, Fouteau, Stéphanie, Gagnevin, Lionel, Guérin, Fabien, Guy, Endrick, Indiana, Arnaud, Koebnik, Ralf, Lauber, Emmanuelle, Munoz, Alejandra, Noel, Laurent D., Pieretti, Isabelle, Poussier, Stéphane, Pruvost, Olivier, Robène-Soustrade, Isabelle, Rott, Philippe, Royer, Monique, Serres-Giardi, Laurana, Szurek, Boris, Van Sluys, Marie-Anne, Verdier, Valérie, Vernière, Christian, Arlat, Mathieu, Manceau, Charles, Jacques, Marie Agnès, Darrasse, Armelle, Carrère, Sébastien, Barbe, Valérie, Boureau, Tristan, Arrieta-Ortiz, Mario L., Bonneau, Sophie, Briand, Martial, Brin, Christelle, Cociancich, Stéphane, Durand, Karine, Fouteau, Stéphanie, Gagnevin, Lionel, Guérin, Fabien, Guy, Endrick, Indiana, Arnaud, Koebnik, Ralf, Lauber, Emmanuelle, Munoz, Alejandra, Noel, Laurent D., Pieretti, Isabelle, Poussier, Stéphane, Pruvost, Olivier, Robène-Soustrade, Isabelle, Rott, Philippe, Royer, Monique, Serres-Giardi, Laurana, Szurek, Boris, Van Sluys, Marie-Anne, Verdier, Valérie, Vernière, Christian, Arlat, Mathieu, Manceau, Charles, and Jacques, Marie Agnès
- Abstract
Background Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences. Results Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations. Conclusions This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness.
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- 2013
36. XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK
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Guy, Endrick, Lautier, Martine, Chabannes, Matthieu, Roux, Brice, Lauber, Emmanuelle, Arlat, Mathieu, Noel, Laurent D., Guy, Endrick, Lautier, Martine, Chabannes, Matthieu, Roux, Brice, Lauber, Emmanuelle, Arlat, Mathieu, and Noel, Laurent D.
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Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc.
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- 2013
37. Genomic survey of pathogenicity determinants and VNTR markers in the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis strain CIO151
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Arrieta-Ortiz, Mario L., Rodríguez-R, Luis Miguel, Pérez-Quintero, Alvaro L., Poulin, Lucie, Díaz, Ana C., Arias Rojas, Nathalia, Trujillo, Cesar, Restrepo Benavides, Mariana, Bart, Rebecca, Boch, Jen, Boureau, Tristan, Darrasse, Armelle, David, Perrine, Dugé de Bernonville, Thomas, Fontanilla, Paula, Gagnevin, Lionel, Guérin, Fabien, Jacques, Marie Agnès, Lauber, Emmanuelle, Lefeuvre, Pierre, Medina, Cesar, Medina, Edgar, Montenegro, Nathaly, Munoz, Alejandra, Noel, Laurent D., Ortiz Quiñoes, Juan F., Osorio, Daniela, Pardo, Carolina, Patil, Prabhu B., Poussier, Stéphane, Pruvost, Olivier, Robène-Soustrade, Isabelle, Ryan, Robert P., Tabima, Javier, Urrego Morales, Osacar G., Vernière, Christian, Carrère, Sébastien, Verdier, Valérie, Szurek, Boris, Restrepo, Silvia, López, Camilo, Koebnik, Ralf, Bernal, Adriana Jimena, Arrieta-Ortiz, Mario L., Rodríguez-R, Luis Miguel, Pérez-Quintero, Alvaro L., Poulin, Lucie, Díaz, Ana C., Arias Rojas, Nathalia, Trujillo, Cesar, Restrepo Benavides, Mariana, Bart, Rebecca, Boch, Jen, Boureau, Tristan, Darrasse, Armelle, David, Perrine, Dugé de Bernonville, Thomas, Fontanilla, Paula, Gagnevin, Lionel, Guérin, Fabien, Jacques, Marie Agnès, Lauber, Emmanuelle, Lefeuvre, Pierre, Medina, Cesar, Medina, Edgar, Montenegro, Nathaly, Munoz, Alejandra, Noel, Laurent D., Ortiz Quiñoes, Juan F., Osorio, Daniela, Pardo, Carolina, Patil, Prabhu B., Poussier, Stéphane, Pruvost, Olivier, Robène-Soustrade, Isabelle, Ryan, Robert P., Tabima, Javier, Urrego Morales, Osacar G., Vernière, Christian, Carrère, Sébastien, Verdier, Valérie, Szurek, Boris, Restrepo, Silvia, López, Camilo, Koebnik, Ralf, and Bernal, Adriana Jimena
- Abstract
Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of bacterial blight of cassava, which is among the main components of human diet in Africa and South America. Current information about the molecular pathogenicity factors involved in the infection process of this organism is limited. Previous studies in other bacteria in this genus suggest that advanced draft genome sequences are valuable resources for molecular studies on their interaction with plants and could provide valuable tools for diagnostics and detection. Here we have generated the first manually annotated high-quality draft genome sequence of Xam strain CIO151. Its genomic structure is similar to that of other xanthomonads, especially Xanthomonas euvesicatoria and Xanthomonas citri pv. citri species. Several putative pathogenicity factors were identified, including type III effectors, cell wall-degrading enzymes and clusters encoding protein secretion systems. Specific characteristics in this genome include changes in the xanthomonadin cluster that could explain the lack of typical yellow color in all strains of this pathovar and the presence of 50 regions in the genome with atypical nucleotide composition. The genome sequence was used to predict and evaluate 22 variable number of tandem repeat (VNTR) loci that were subsequently demonstrated as polymorphic in representative Xam strains. Our results demonstrate that Xanthomonas axonopodis pv. manihotis strain CIO151 possesses ten clusters of pathogenicity factors conserved within the genus Xanthomonas. We report 126 genes that are potentially unique to Xam, as well as potential horizontal transfer events in the history of the genome. The relation of these regions with virulence and pathogenicity could explain several aspects of the biology of this pathogen, including its ability to colonize both vascular and non-vascular tissues of cassava plants. A set of 16 robust, polymorphic VNTR loci will be useful to develop a multi-locus VNTR analysis s
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- 2013
38. Genomic Survey of Pathogenicity Determinants and VNTR Markers in the Cassava Bacterial Pathogen Xanthomonas axonopodis pv. Manihotis Strain CIO151
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Arrieta-Ortiz, Mario L., primary, Rodríguez-R, Luis M., additional, Pérez-Quintero, Álvaro L., additional, Poulin, Lucie, additional, Díaz, Ana C., additional, Arias Rojas, Nathalia, additional, Trujillo, Cesar, additional, Restrepo Benavides, Mariana, additional, Bart, Rebecca, additional, Boch, Jens, additional, Boureau, Tristan, additional, Darrasse, Armelle, additional, David, Perrine, additional, Dugé de Bernonville, Thomas, additional, Fontanilla, Paula, additional, Gagnevin, Lionel, additional, Guérin, Fabien, additional, Jacques, Marie-Agnès, additional, Lauber, Emmanuelle, additional, Lefeuvre, Pierre, additional, Medina, Cesar, additional, Medina, Edgar, additional, Montenegro, Nathaly, additional, Muñoz Bodnar, Alejandra, additional, Noël, Laurent D., additional, Ortiz Quiñones, Juan F., additional, Osorio, Daniela, additional, Pardo, Carolina, additional, Patil, Prabhu B., additional, Poussier, Stéphane, additional, Pruvost, Olivier, additional, Robène-Soustrade, Isabelle, additional, Ryan, Robert P., additional, Tabima, Javier, additional, Urrego Morales, Oscar G., additional, Vernière, Christian, additional, Carrere, Sébastien, additional, Verdier, Valérie, additional, Szurek, Boris, additional, Restrepo, Silvia, additional, López, Camilo, additional, Koebnik, Ralf, additional, and Bernal, Adriana, additional
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- 2013
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39. Genome sequence of Xanthomonas fuscans subsp. fuscansstrain 4834-R reveals that flagellar motility is not a general feature of xanthomonads
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Darrasse, Armelle, primary, Carrère, Sébastien, additional, Barbe, Valérie, additional, Boureau, Tristan, additional, Arrieta-Ortiz, Mario L, additional, Bonneau, Sophie, additional, Briand, Martial, additional, Brin, Chrystelle, additional, Cociancich, Stéphane, additional, Durand, Karine, additional, Fouteau, Stéphanie, additional, Gagnevin, Lionel, additional, Guérin, Fabien, additional, Guy, Endrick, additional, Indiana, Arnaud, additional, Koebnik, Ralf, additional, Lauber, Emmanuelle, additional, Munoz, Alejandra, additional, Noël, Laurent D, additional, Pieretti, Isabelle, additional, Poussier, Stéphane, additional, Pruvost, Olivier, additional, Robène-Soustrade, Isabelle, additional, Rott, Philippe, additional, Royer, Monique, additional, Serres-Giardi, Laurana, additional, Szurek, Boris, additional, van Sluys, Marie-Anne, additional, Verdier, Valérie, additional, Vernière, Christian, additional, Arlat, Matthieu, additional, Manceau, Charles, additional, and Jacques, Marie-Agnès, additional
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- 2013
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40. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels
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Pieretti, Isabelle, Royer, Monique, Barbe, Valérie, Carrère, Sébastien, Koebnik, Ralf, Couloux, Arnaud, Darrasse, Armelle, Gouzy, Jérôme, Jacques, Marie Agnès, Lauber, Emmanuelle, Manceau, Charles, Mangenot, Sophie, Poussier, Stéphane, Segurens, Béatrice, Szurek, Boris, Verdier, Valérie, Arlat, Mathieu, Gabriel, Dean W., Rott, Philippe, Cociancich, Stéphane, Pieretti, Isabelle, Royer, Monique, Barbe, Valérie, Carrère, Sébastien, Koebnik, Ralf, Couloux, Arnaud, Darrasse, Armelle, Gouzy, Jérôme, Jacques, Marie Agnès, Lauber, Emmanuelle, Manceau, Charles, Mangenot, Sophie, Poussier, Stéphane, Segurens, Béatrice, Szurek, Boris, Verdier, Valérie, Arlat, Mathieu, Gabriel, Dean W., Rott, Philippe, and Cociancich, Stéphane
- Abstract
Background Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of Xanthomonas. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic X. albilineans strain GPE PC73 focused exclusively on distinctive genomic features shared with Xylella fastidiosa-another xylem-limited Xanthomonadaceae. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of X. albilineans, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity. Results Comparative genomic analyses showed that most of the known pathogenicity factors from other Xanthomonas species are conserved in X. albilineans, with the notable absence of two major determinants of the "artillery" of other plant pathogenic species of Xanthomonas: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to X. albilineans that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to X. albilineans were identi
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- 2012
41. The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae
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Pieretti, Isabelle, Royer, Monique, Barbe, Valérie, Carrère, Sébastien, Koebnik, Ralf, Cociancich, Stéphane, Couloux, Arnaud, Darrasse, Armelle, Gouzy, Jérôme, Jacques, Marie Agnès, Lauber, Emmanuelle, Manceau, Charles, Mangenot, Sophie, Poussier, Stéphane, Segurens, Béatrice, Szurek, Boris, Verdier, Véronique, Arlat, Mathieu, Rott, Philippe, Pieretti, Isabelle, Royer, Monique, Barbe, Valérie, Carrère, Sébastien, Koebnik, Ralf, Cociancich, Stéphane, Couloux, Arnaud, Darrasse, Armelle, Gouzy, Jérôme, Jacques, Marie Agnès, Lauber, Emmanuelle, Manceau, Charles, Mangenot, Sophie, Poussier, Stéphane, Segurens, Béatrice, Szurek, Boris, Verdier, Véronique, Arlat, Mathieu, and Rott, Philippe
- Abstract
Background. The Xanthomonadaceae family contains two xylem-limited plant pathogenic bacterial species, Xanthomonas albilineans and Xylella fastidiosa. X. fastidiosa was the first completely sequenced plant pathogen. It is insect-vectored, has a reduced genome and does not possess hrp genes which encode a Type III secretion system found in most plant pathogenic bacteria. X. fastidiosa was excluded from the Xanthomonas group based on phylogenetic analyses with rRNA sequences. Results. The complete genome of X. albilineans was sequenced and annotated. X. albilineans, which is not known to be insect-vectored, also has a reduced genome and does not possess hrp genes. Phylogenetic analysis using X. albilineans genomic sequences showed that X. fastidiosa belongs to the Xanthomonas group. Order of divergence of the Xanthomonadaceae revealed that X. albilineans and X. fastidiosa experienced a convergent reductive genome evolution during their descent from the progenitor of the Xanthomonas genus. Reductive genome evolutions of the two xylem-limited Xanthomonadaceae were compared in light of their genome characteristics and those of obligate animal symbionts and pathogens. Conclusion. The two xylem-limited Xanthomonadaceae, during their descent from a common ancestral parent, experienced a convergent reductive genome evolution. Adaptation to the nutrient-poor xylem elements and to the cloistered environmental niche of xylem vessels probably favoured this convergent evolution. However, genome characteristics of X. albilineans differ from those of X. fastidiosa and obligate animal symbionts and pathogens, indicating that a distinctive process was responsible for the reductive genome evolution in this pathogen. The possible role in genome reduction of the unique toxin albicidin, produced by X. albilineans, is discussed.
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- 2009
42. xopAC-triggered Immunity against Xanthomonas Depends on Arabidopsis Receptor-Like Cytoplasmic Kinase Genes PBL2 and RIPK
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Guy, Endrick, primary, Lautier, Martine, additional, Chabannes, Matthieu, additional, Roux, Brice, additional, Lauber, Emmanuelle, additional, Arlat, Matthieu, additional, and Noël, Laurent D., additional
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- 2013
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43. The xylan utilization system of the plant pathogenX anthomonas campestrispv campestris controls epiphytic life and reveals common features with oligotrophic bacteria and animal gut symbionts
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Déjean, Guillaume, primary, Blanvillain‐Baufumé, Servane, additional, Boulanger, Alice, additional, Darrasse, Armelle, additional, Bernonville, Thomas Dugé, additional, Girard, Anne‐Laure, additional, Carrére, Sébastien, additional, Jamet, Stevie, additional, Zischek, Claudine, additional, Lautier, Martine, additional, Solé, Magali, additional, Büttner, Daniela, additional, Jacques, Marie‐Agnès, additional, Lauber, Emmanuelle, additional, and Arlat, Matthieu, additional
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- 2013
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44. Plant carbohydrate scavenging through TonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria
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Blanvillain, Servane, Meyer, Damien, Boulanger, Alice, Lautier, Martine, Guynet, Catherine, Denancé, Nicolas, Vasse, Jacques, Lauber, Emmanuelle, Arlat, Mathieu, Blanvillain, Servane, Meyer, Damien, Boulanger, Alice, Lautier, Martine, Guynet, Catherine, Denancé, Nicolas, Vasse, Jacques, Lauber, Emmanuelle, and Arlat, Mathieu
- Abstract
TonB-dependent receptors (TBDRs) are outer membrane proteins mainly known for the active transport of iron siderophore complexes in Gram-negative bacteria. Analysis of the genome of the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), predicts 72 TBDRs. Such an overrepresentation is common in Xanthomonas species but is limited to only a small number of bacteria. Here, we show that one Xcc TBDR transports sucrose with a very high affinity, suggesting that it might be a sucrose scavenger. This TBDR acts with an inner membrane transporter, an amylosucrase and a regulator to utilize sucrose, thus defining a new type of carbohydrate utilization locus, named CUT locus, involving a TBDR for the transport of substrate across the outer membrane. This sucrose CUT locus is required for full pathogenicity on Arabidopsis, showing its importance for the adaptation to host plants. A systematic analysis of Xcc TBDR genes and a genome context survey suggested that several Xcc TBDRs belong to other CUT loci involved in the utilization of various plant carbohydrates. Interestingly, several Xcc TBDRs and CUT loci are conserved in aquatic bacteria such as Caulobacter crescentus, Colwellia psychrerythraea, Saccharophagus degradans, Shewanella spp., Sphingomonas spp. or Pseudoalteromonas spp., which share the ability to degrade a wide variety of complex carbohydrates and display TBDR overrepresentation. We therefore propose that TBDR overrepresentation and the presence of CUT loci designate the ability to scavenge carbohydrates. Thus CUT loci, which seem to participate to the adaptation of phytopathogenic bacteria to their host plants, might also play a very important role in the biogeochemical cycling of plant-derived nutrients in marine environments. Moreover, the TBDRs and CUT loci identified in this study are clearly different from those characterized in the human gut symbiont Bacteroides thetaiotaomicron, which allow glycan foraging, suggesting a convergent evol
- Published
- 2007
45. Optimization of pathogenicity assays to study the Arabidopsis thaliana-Xanthomonas campestris pv. campastris pathosystem
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Meyer, Damien, Lauber, Emmanuelle, Roby, Dominique, Arlat, Mathieu, Kroj, Thomas, Meyer, Damien, Lauber, Emmanuelle, Roby, Dominique, Arlat, Mathieu, and Kroj, Thomas
- Abstract
The cruciferous weed Arabidopsis thaliana and the causal agent of black rot disease of Crucifers Xanthomonas campestris pv. campestris ( Xcc ) are both model organisms in plant pathology. Their interaction has been studied successfully in the past, but these investigations suffered from high variability. In the present study, we describe an improved Arabidopsis- Xcc pathosystem that is based on a wound inoculation procedure. We show that after wound inoculation, Xcc colonizes the vascular system of Arabidopsis leaves and causes typical black rot symptoms in a compatible interaction, while in an incompatible interaction bacterial multiplication is inhibited. The highly synchronous and reproducible symptom expression allowed the development of a disease scoring scheme that enabled us to analyse the effects of mutations in individual genes on plant resistance or on bacterial virulence in a simple and precise manner. This optimized Arabidopsis- Xcc pathosystem will be a robust tool for further genetic and post-genomic investigation of fundamental questions in plant pathology.
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- 2005
46. Insights into the Extracytoplasmic Stress Response of Xanthomonas campestris pv. campestris : Role and Regulation of σ E -Dependent Activity
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Bordes, Patricia, primary, Lavatine, Laure, additional, Phok, Kounthéa, additional, Barriot, Roland, additional, Boulanger, Alice, additional, Castanié-Cornet, Marie-Pierre, additional, Déjean, Guillaume, additional, Lauber, Emmanuelle, additional, Becker, Anke, additional, Arlat, Matthieu, additional, and Gutierrez, Claude, additional
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- 2011
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47. The complete genome sequence of Xanthomonas albilineans provides new insights into the reductive genome evolution of the xylem-limited Xanthomonadaceae
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Pieretti, Isabelle, primary, Royer, Monique, additional, Barbe, Valérie, additional, Carrere, Sébastien, additional, Koebnik, Ralf, additional, Cociancich, Stéphane, additional, Couloux, Arnaud, additional, Darrasse, Armelle, additional, Gouzy, Jérôme, additional, Jacques, Marie-Agnès, additional, Lauber, Emmanuelle, additional, Manceau, Charles, additional, Mangenot, Sophie, additional, Poussier, Stéphane, additional, Segurens, Béatrice, additional, Szurek, Boris, additional, Verdier, Valérie, additional, Arlat, Matthieu, additional, and Rott, Philippe, additional
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- 2009
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48. AvrAC Xcc8004 , a Type III Effector with a Leucine-Rich Repeat Domain from Xanthomonas campestris Pathovar campestris Confers Avirulence in Vascular Tissues of Arabidopsis thaliana Ecotype Col-0
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Xu, Rong-Qi, primary, Blanvillain, Servane, additional, Feng, Jia-Xun, additional, Jiang, Bo-Le, additional, Li, Xian-Zhen, additional, Wei, Hong-Yu, additional, Kroj, Thomas, additional, Lauber, Emmanuelle, additional, Roby, Dominique, additional, Chen, Baoshan, additional, He, Yong-Qiang, additional, Lu, Guang-Tao, additional, Tang, Dong-Jie, additional, Vasse, Jacques, additional, Arlat, Matthieu, additional, and Tang, Ji-Liang, additional
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- 2008
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49. Plant Carbohydrate Scavenging through TonB-Dependent Receptors: A Feature Shared by Phytopathogenic and Aquatic Bacteria
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Blanvillain, Servane, primary, Meyer, Damien, additional, Boulanger, Alice, additional, Lautier, Martine, additional, Guynet, Catherine, additional, Denancé, Nicolas, additional, Vasse, Jacques, additional, Lauber, Emmanuelle, additional, and Arlat, Matthieu, additional
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- 2007
- Full Text
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50. Optimization of pathogenicity assays to study theArabidopsis thaliana-Xanthomonas campestrispv.campestrispathosystem
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MEYER, DAMIEN, primary, LAUBER, EMMANUELLE, additional, ROBY, DOMINIQUE, additional, ARLAT, MATTHIEU, additional, and KROJ, THOMAS, additional
- Published
- 2005
- Full Text
- View/download PDF
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