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2. Flavoenzyme und ein FMN-Riboswitch aus Escherichia coli sind Zielmoleküle für das Riboflavinanalogon Roseoflavin aus Streptomyces davawensis

Author

Langer, Simone Nicole and Fischer, R.

Subjects
Life sciences, biology, Roseoflavin, Flavoenzyme, Riboswitch, ddc:570, Riboflavin, Azobenzolreduktase
Abstract

Das Riboflavinanalogon Roseoflavin wird von Streptomyces davawensis produziert und in Zielzellen zu Roseoflavin-5'-mononukleotid (RoFMN) und Roseoflavinadenindinukleotid (RoFAD) umgesetzt. Flavoenzyme und ein FMN-Riboswitch aus Escherichia coli wurden als mögliche Zielmoleküle für Roseoflavin untersucht. 37 von 38 untersuchten Flavoenzymen konnten RoFMN/RoFAD binden. Die beispielhaft untersuchte Azobenzolreduktase AzoR aus E. coli hatte eine um 70 % reduzierte Aktivität in der RoFMN-Form. Auch der Riboswitch sroG aus E. coli reagierte auf RoFMN.

Published
2013
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4. Flavoproteins Are Potential Targets for the Antibiotic Roseoflavin in Escherichia coli.

Author

Langer, Simone, Hashimoto, Masayuki, Hobl, Birgit, Mathes, Tilo, and Mack, Matthias

Subjects
*VITAMIN B2, *ANTIBIOTICS, *STREPTOMYCES, *LIGASES, *FLAVOPROTEINS, *CYTOPLASM
Abstract

The riboflavin analog roseoflavin is an antibiotic produced by Streptomyces davawensis. Riboflavin transporters are responsible for roseoflavin uptake by target cells. Roseoflavin is converted to the flavin mononucleotide (FMN) analog roseoflavin mononucleotide (RoFMN) by flavokinase and to the flavin adenine dinucleotide (FAD) analog roseoflavin adenine dinucleotide (RoFAD by FAD synthetase. In order to study the effect of RoFMN and RoFAD in the cytoplasm of target cells, Escherichia coli was used as a model. E. coli is predicted to contain 38 different FMN- or FAD-dependent proteins (flavoproteins). These proteins were overproduced in recombinant E. coli strains grown in the presence of sublethal amounts of roseoflavin. The flavoproteins were purified and analyzed with regard to their cofactor contents. It was found that 37 out of 38 flavoproteins contained either RoFMN or RoFAD. These cofactors have different physicochemical properties than FMN and FAD and were reported to reduce or completely abolish flavoprotein function. [ABSTRACT FROM AUTHOR]

Published
2013
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