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The use of synthetic antioxidants has been restricted in the current scenario due to their hazardous and carcinogenic effects. As a result, a hunt for natural antioxidants is critical. Natural chemicals are of interest for research because of their safe and healthier implications and minimal toxicity. The aim of this study is to analyse the antioxidant potential of Nyctanthes leaves which may be attributed to large amounts of phenolic constituents present in the leaves of this traditionally valuable plant. The ethanolic extract of the leaves of Nyctanthesarbor-tristris was tested by in vitro antioxidant method using DPPH (1,1-diphenyl-2-picrylhydrazine). The extract was taken in five different concentrations ranging from 100-500 μg/ml. The results obtained were comparable to the standard ascorbic acid. This alludes that Nyctanthes leaves can be used as a potential source of free radical scavenging activity. [ABSTRACT FROM AUTHOR]