1. Voltage-dependent interaction of the peptaibol antibiotic zervamicin II with phospholipid vesicles.
- Author
-
Kropacheva TN and Raap J
- Subjects
- Alamethicin pharmacology, Amino Acid Sequence, Anti-Bacterial Agents pharmacology, Electric Conductivity, Ion Channels drug effects, Membrane Potentials drug effects, Molecular Sequence Data, Peptaibols, Valinomycin pharmacology, Anti-Bacterial Agents metabolism, Ion Channels physiology, Peptides, Phospholipids physiology
- Abstract
The effect of a transmembrane potential on ion channel formation by zervamicin II (ZER-II) was studied in a vesicular model system. The dissipation of diffusion potential caused by addition of ZER-II to small phosphatidylcholine vesicles was monitored using fluorescent (Safranine T) and optical (Oxonol YI) probes. Cis-positive potentials facilitated channel formation, while at cis-negative potentials, ion fluxes were inhibited. A potential-independent behavior of ZER-II was observed at high peptide concentrations, most likely due to its membrane modifying property.
- Published
- 1999
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