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Your search keyword '"Krejčí, Jana"' showing total 20 results
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20 results on '"Krejčí, Jana"'

5. Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis.

Author

KREJČÍ, Jana, ARCIDIACONO, Orazio Angelo, ČEGAN, Radim, RADASZKIEWICZ, Katarzyna, PACHERNÍK, Jiří, PIRK, Jan, PEŠL, Martin, FILA, Petr, and BÁRTOVÁ, Eva

Subjects
AGING, CELL differentiation, EPIGENETICS, HEART development, DEMETHYLATION
Abstract

FTO and ALKBH5 proteins are essential erasers of N6-adenosine methylation in RNA. We studied how levels of FTO and ALKBH5 proteins changed during mouse embryonic development, aging, cardiomyogenesis, and neuroectodermal differentiation. We observed that aging in male and female mice was associated with FTO up-regulation in mouse hearts, brains, lungs, and kidneys, while the ALKBH5 level remained stable. FTO and ALKBH5 proteins were up-regulated during experimentally induced cardiomyogenesis, but the level of ALKBH5 protein was not changed when neuroectodermal differentiation was induced. HDAC1 depletion in mouse ES cells caused FTO down-regulation. In these cells, mRNA, carrying information from genes that regulate histone signature, RNA processing, and cell differentiation, was characterized by a reduced level of N6-adenosine methylation in specific gene loci, primarily regulating cell differentiation into neuroectoderm. Together, when we compared both RNA demethylating proteins, the FTO protein level undergoes the most significant changes during cell differentiation and aging. Thus, we conclude that during aging and neuronal differentiation, m6A RNA demethylation is likely regulated by the FTO protein but not via the function of ALKBH5. [ABSTRACT FROM AUTHOR]

Published
2023
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13. MCDM methods based on pairwise comparison matrices and their fuzzy extension

Author

Fedrizzi, Michele, Siebert, Johannes, Krejčí, Jana, Fedrizzi, Michele, Siebert, Johannes, and Krejčí, Jana

Abstract

Methods based on pairwise comparison matrices (PCMs) form a significant part of multi-criteria decision making (MCDM) methods. These methods are based on structuring pairwise comparisons (PCs) of objects from a finite set of objects into a PCM and deriving priorities of objects that represent the relative importance of each object with respect to all other objects in the set. However, the crisp PCMs are not able to capture uncertainty stemming from subjectivity of human thinking and from incompleteness of information about the problem that are often closely related to MCDM problems. That is why the fuzzy extension of methods based on PCMs has been of great interest. In order to derive fuzzy priorities of objects from a fuzzy PCM (FPCM), standard fuzzy arithmetic is usually applied to the fuzzy extension of the methods originally developed for crisp PCMs. %Fuzzy extension of the methods based on PCMs usually consists in simply replacing the crisp PCs in the given model by fuzzy PCs and applying standard fuzzy arithmetic to obtain the desired fuzzy priorities. However, such approach fails in properly handling uncertainty of preference information contained in the FPCM. Namely, reciprocity of the related PCs of objects in a FPCM and invariance of the given method under permutation of objects are violated when standard fuzzy arithmetic is applied to the fuzzy extension. This leads to distortion of the preference information contained in the FPCM and consequently to false results. Thus, the first research question of the thesis is: ``Based on a FPCM of objects, how should fuzzy priorities of these objects be determined so that they reflect properly all preference information available in the FPCM?'' This research question is answered by introducing an appropriate fuzzy extension of methods originally developed for crisp PCMs. That is, such fuzzy extension that does not violate reciprocity of the related PCs and invariance under permutation of objects, and that does not l

Published
2017

20. Lamin A/C and Polymeric Actin in Genome Organization.

Author

Ondřej, Vladan, Lukášová, Emilie, Krejčí, Jana, Matula, Pavel, and Kozubek, Stanislav

Abstract

In this work, we have studied the structural and functional linkage between lamin A/C, nuclear actin, and organization of chromosome territories (CTs) in mammary carcinoma MCF-7 cells. Selective down-regulation of lamin A/C expression led to disruption of the lamin A/C perinuclear layer and disorganization of lamin-bound emerin complexes at the inner nuclear membrane. The silencing of lamin A/C expression resulted in a decrease in the volume and surface area of chromosome territories, especially in chromosomes with high heterochromatin content. Inhibition of actin polymerization led to relaxation of the structure of chromosome territories, and an increase in the volumes and surface areas of the chromosome territories of human chromosomes 1, 2 and 13. The results show an important role of polymeric actin in the organization of the nuclei and the chromosome territories. [ABSTRACT FROM AUTHOR]

Published
2008
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