9 results on '"Koglin K"'
Search Results
2. Blue and red in the protein world: Photoactive yellow protein and phytochromes as revealed by time-resolved crystallography.
- Author
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Schmidt, Marius and Stojković, Emina A.
- Subjects
PHOTOACTIVE yellow protein ,ULTRASHORT laser pulses ,FREE electron lasers ,CRYSTALLOGRAPHY ,PHYTOCHROMES ,X-ray lasers ,BIOMACROMOLECULES ,PHOTORECEPTORS - Abstract
Time-resolved crystallography (TRX) is a method designed to investigate functional motions of biological macromolecules on all time scales. Originally a synchrotron-based method, TRX is enabled by the development of TR Laue crystallography (TRLX). TR serial crystallography (TR-SX) is an extension of TRLX. As the foundations of TRLX were evolving from the late 1980s to the turn of the millennium, TR-SX has been inspired by the development of Free Electron Lasers for hard X-rays. Extremely intense, ultrashort x-ray pulses could probe micro and nanocrystals, but at the same time, they inflicted radiation damage that necessitated the replacement by a new crystal. Consequently, a large number of microcrystals are exposed to X-rays one by one in a serial fashion. With TR-SX methods, one of the largest obstacles of previous approaches, namely, the unsurmountable challenges associated with the investigation of non-cyclic (irreversible) reactions, can be overcome. This article describes successes and transformative contributions to the TRX field by Keith Moffat and his collaborators, highlighting two major projects on protein photoreceptors initiated in the Moffat lab at the turn of the millennium. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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3. Stimulation of soluble guanylate cyclase exerts antiinflammatory actions in the liver through a VASP/NF-κB/NLRP3 inflammasome circuit.
- Author
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Flores-Costa, Roger, Duran-Güell, Marta, Casulleras, Mireia, López-Vicario, Cristina, Alcaraz-Quiles, José, Diaz, Alba, Lozano, Juan J., Titos, Esther, Hall, Katherine, Sarno, Renee, Masferrer, Jaime L., and Clària, Joan
- Subjects
GUANYLATE cyclase ,CGMP-dependent protein kinase ,CELL communication ,KUPFFER cells ,GUANOSINE triphosphate - Abstract
Soluble guanylate cyclase (sGC) catalyzes the conversion of guanosine triphosphate into cyclic guanosine-3',5'-monophosphate, a key second messenger in cell signaling and tissue homeostasis. It was recently demonstrated that sGC stimulation is associated with a marked antiinflammatory effect in the liver of mice with experimental nonalcoholic steatohepatitis (NASH). Here, we investigated the mechanisms underlying the antiinflammatory effect of the sGC stimulator praliciguat (PRL) in the liver. Therapeutic administration of PRL exerted antiinflammatory and antifibrotic actions in mice with choline-deficient L-amino acid-defined high-fat diet-induced NASH. The PRL antiinflammatory effect was associated with lower F4/80- and CX3CR1-positive macrophage infiltration into the liver in parallel with lower Ly6CHigh- and higher Ly6CLow-expressing monocytes in peripheral circulation. The PRL antiinflammatory effect was also associated with suppression of hepatic levels of interleukin (IL)-1β, NLPR3 (NACHT, LRR, and PYD domain-containing protein 3), ASC (apoptosis-associated specklike protein containing a caspase-recruitment domain), and active cleaved-caspase-1, which are components of the NLRP3 inflammasome. In Kupffer cells challenged with the classical inflammasome model of lipopolysaccharide plus adenosine triphosphate, PRL inhibited the priming (expression of Il1b and Nlrp3) and blocked the release of mature IL-1β. Mechanistically, PRL induced the protein kinase G (PKG)-mediated phosphorylation of the VASP (vasodilator-stimulated phosphoprotein) Ser239 residue which, in turn, reduced nuclear factor-κB (NF-κB) activity and Il1b and Nlrp3 gene transcription. PRL also reduced active cleaved-caspase-1 levels independent of pannexin-1 activity. These data indicate that sGC stimulation with PRL exerts antiinflammatory actions in the liver through mechanisms related to a PKG/VASP/NF-κB/NLRP3 inflammasome circuit. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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4. Blastulation of a zygote to a hatched blastocyst without any clear cell division: an observational finding in a time-lapse system after in vitro fertilization.
- Author
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Sandi-Monroy, N.L., Musanovic, S., Zhu, D., Eibner, K., Reeka, N., Koglin, J., Bundschu, K., and Gagsteiger, F.
- Subjects
FERTILIZATION in vitro ,ZYGOTES ,CELL division ,EMBRYOLOGY ,BLASTOCYST ,INDUCED ovulation - Abstract
Purpose: To describe an interesting not previously described morphokinetic finding. Methods: Retrospective case report of a couple undergoing controlled ovarian stimulation (COS) followed by in vitro fertilization and blastocyst transfer. Results: We identified a unique finding of blastulation of a fertilized human zygote after conventional in vitro fertilization. The fertilized zygote did not show any clear cytokinesis until approximately 107 h post insemination, when it started dividing into a blastocyst. By 113 h post insemination, inner cell mass and trophectoderm cells could be clearly distinguished and the blastocyst was completely hatched by 136 h post insemination. Conclusion: Time-lapse systems offer more detailed observations of embryonic development. Here, we report an atypical development of an embryo that was not described previously. We hope to become an insightful discussion among peers and incentive the publication of such findings in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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5. Recent developments in joining of aluminum alloys.
- Author
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Çam, Gürel and İpekoğlu, Güven
- Subjects
JOINING processes ,ALUMINUM alloys ,FABRICATION (Manufacturing) ,LIGHT metal alloys ,FRICTION stir welding - Abstract
The mass saving potential of light-weight materials, such as Al alloys, is beneficial for fuel economy and reducing CO emissions. However, the wide-spread use of these alloys has been long hindered due to the difficulty in fusion joining as well as their high cost. Welding of Al alloys, which are considered to be difficult to weld through conventional arc welding, is now possible by either of low heat input arc welding, high-power density fusion joining, such as laser beam welding and electron beam welding, or friction stir welding. Particularly, friction stir welding can be successfully applied to these materials owing to the fact that no melting takes place in the weld nugget. The aim of this overview is to summarize the developments in the joining of Al alloys over the recent years. This study is also intended to provide guidance for the industry and researchers dealing with joining of these alloys. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Структурные и рентгено-оптические характеристики многослойных рентгеновских зеркал W/Si
- Author
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Першин, Ю. П., Девизенко, А. Ю., Кондратенко, В. В., Modrow, H., and Hormes, F.-J.
- Abstract
Copyright of Metallophysics & Advanced Technologies / Metallofizika i Novejsie Tehnologii is the property of G.V. Kurdyumov Institute for Metal Physics, N.A.S.U and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2016
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7. LIGHTWEIGHT DESIGN OF VEHICLE BODY A CONTRIBUTION TOWARD GREENER ENVIRONMENT.
- Author
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BELINGARDI, Giovanni
- Subjects
LIGHTWEIGHT construction ,AUTOMOBILES ,LIGHT metal alloys ,COMPOSITE materials ,AUTOMOTIVE engineering - Abstract
The vehicle mass experienced a period of progressive increase. Nowadays as it is essential to decrease both the fuel consumption and the CO
2 production, lightweight design becomes a relevant target for car manufacturer. One of the considered strategy toward lightweight is the substitution of the material used for manufacturing the different parts of the car body. Together with the new developed HSS steels, aluminium and magnesium are considerded. Recently composite material solutions have gained a lot of attention due to their very interesting characteristic. The paper discusses some the relevant aspects when developing a new material solution from the material production to the end-of-life. The paper includes one example of results that can be achieved with properly oriented light weight design procedure. [ABSTRACT FROM AUTHOR]- Published
- 2014
8. Atomic Layer Deposition Re Ective Coatings For Future Astronomical Space Telescopes And The Solar Corona Viewed Through The Minxss (Miniature X-Ray Solar Spectrometer) Cubesats
- Author
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Christopher Samuel Moore, Kevin C. France, and Thomas N. Woods
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detector ,reflectance ,Sun ,atomic layer etch ,coatings ,x-rays ,flares ,active region ,thin films ,ultraviolet ,synchrotron ,atomic layer deposition ,quiet Sun ,mirror ,minxss ,cubesat ,coronae - Abstract
Advances in technology and instrumentation open new windows for observing astrophysical objects. The first half of my dissertation involves the development of atomic layer deposition (ALD) coatings to create high reflectivity UV mirrors for future satellite astronomical telescopes. Aluminum (Al) has intrinsic reflectance greater than 80% from 90 – 2,000 nm, but develops a native aluminum oxide (Al2O3) layer upon exposure to air that readily absorbs light below 250 nm. Thus, Al based UV mirrors must be protected by a transmissive overcoat. Traditionally, metal-fluoride overcoats such as MgF2 and LiF are used to mitigate oxidation but with caveats. We utilize a new metal fluoride (AlF3) to protect Al mirrors deposited by ALD. ALD allows for precise thickness control, conformal and near stoichiometric thin films. We prove that depositing ultra-thin (~3 nm) ALD ALF3 to protect Al mirrors after removing the native oxide layer via atomic layer etching (ALE) enhances the reflectance near 90 nm from ~5% to ~30%. X-ray detector technology with high readout rates are necessary for the relatively bright Sun, particularly during large flares. The hot plasma in the solar corona generates X-rays, which yield information on the physical conditions of the plasma. The second half of my dissertation includes detector testing, characterization and solar science with the Miniature X-ray Solar Spectrometer (MinXSS) CubeSats. The MinXSS CubeSats employ Silicon Drift Diode (SDD) detectors called X123, which generate full sun spectrally resolved (~0.15 FWHM at 5.9 keV) measurements of the sparsely measured, 0.5 – 12 keV range. The absolute radiometric calibration of the MinXSS instrument suite was performed at the National Institute for Standards and Technology (NIST) Synchrotron Ultraviolet Radiation Facility (SURF) and spectral resolution determined from radioactive sources. I used MinXSS along with data from the Geostationary Operational Environmental Satellites (GOES), Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI), Hinode X-ray Telescope (XRT), Hinode Extreme Ultraviolet Imaging Spectrometer (EIS) and Solar Dynamics Observatory (SDO) Atmospheric Imaging Assembly (AIA) to study the solar corona. This resulted in new insights on the coronal temperature distribution and elemental abundance variations for quiescence, active regions and during solar flares., {"references": ["B. P. Abbott, R. Abbott, T. D. Abbott, M. R. Abernathy, F. Acernese, K. Ackley, C. Adams, T. Adams, P. Addesso, R. X. Adhikari, and et al. Observation of Gravitational Waves from a Binary Black Hole Merger. 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9. New insights from cosmic gamma rays.
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Diehl Roland
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