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3. Civil.

Author

Klein, Alexis and Buehler, Vincent G.

Subjects
Civil procedure -- Surveys
Published
2009

5. ATP-binding cassette transporter A1 is significantly involved in the intestinal absorption of [alpha]- and [gamma]-tocopherol but not in that of retinyl palmitate in mice

Author

Reboul, Emmanuelle, Trompier, Doriane, Moussa, Myriam, Klein, Alexis, Landrier, Jean-Francois, Chimini, Giovanna, and Borel, Patrick

Subjects
Intestinal absorption -- Health aspects, Membrane proteins -- Health aspects, Vitamin A -- Health aspects, Vitamin E -- Health aspects, Food/cooking/nutrition, Health
Abstract

Background: It has long been assumed that newly absorbed vitamin A and E enter the body only via enterocyte-produced chylomicrons. However, recent results in cell cultures have shown that a fraction of [alpha]-tocopherol is secreted with intestinal HDL. Objectives: The aims of this study were to identify this transporter and to assess whether it is significantly implicated in the in vivo intestinal absorption of the 2 main dietary forms of vitamin E (ie, [alpha]-and [gamma]-tocopherol) and in that of retinyl palmitate (vitamin A). Design: Having performed preliminary experiments in the Caco-2 cell model, we compared fasting and postprandial plasma concentrations of vitamins A and E in mice deficient in ATP-binding cassette A1 (ABCA1) transporter and in wild-type mice. Results: A substantial efflux of [alpha]- and [gamma]-tocopherol, but not of retinyl esters, was induced by the presence of apolipoprotein A-I at the basolateral side of Caco-2 monolayers. The efflux of [alpha]- and [gamma]-tocopherol was also impaired by glyburide and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The postprandial response of plasma [gamma]-tocopherol was 4-fold lower in [ABCA1.sup.-/-] mice (P = 0.025) than in wild-type mice, whereas no significant difference was observed for retinyl esters. Fasting plasma [alpha]-tocopherol, but not vitamin A, concentrations were lower in mice bearing the genetic deletion. Conclusions: ABCA1 is the transporter responsible for the in vivo secretion of [alpha]- and [gamma]-tocopherol with intestinal HDL, and this pathway is significantly implicated in the intestinal absorption and plasma status of vitamin E but not of vitamin A.

Published
2009

7. Alterations in plasma vitamin E distribution in type 2 diabetic patients with elevated plasma phospholipid transfer protein activity

Author

Duettmann, Martina, Verges, Bruno, Klein, Alexis, Miller, Elizabeth R., Deckert, Valerie, Desrumaux, Catherine, Masson, David, Gambert, Philippe, Brun, Jean-Marcel, Fruchart-Najib, Jamila, Blache, Denis, Witztum, Joseph L., and Lagrost, Laurent

Subjects
Vitamin E -- Nutritional aspects -- Research, Phospholipids -- Research -- Nutritional aspects, Diabetes -- Care and treatment -- Research, Health, Care and treatment, Nutritional aspects, Research
Abstract

Mouse studies indicated that plasma phospholipid transfer protein (PLTP) determines the plasma distribution of vitamin E, a potent lipophilic antioxidant. Vitamin E distribution, antioxidant status, and titer of anti-oxidized LDLs (oxLDL) autoantibodies were evaluated in plasma from control subjects (n = 31) and type 2 diabetic patients (n = 31) with elevated plasma PLTP concentration. Unlike diabetic and control HDLs, which displayed similar vitamin E contents, diabetic VLDLs and diabetic LDLs contained fewer vitamin E molecules than normal counterparts. Plasma PLTP concentration in diabetic plasmas correlated negatively with vitamin E in VLDL+LDL, but positively with vitamin E in HDL, with an even stronger correlation with the VLDL+LDL-to-HDL vitamin E ratio. Circulating levels of oxLDL were significantly higher in diabetic plasmas than in control plasmas. Whereas the titer of IgG autoantibodies to modified LDL did not differ significantly between diabetic patients and control subjects, diabetic plasmas showed significantly lower levels of potentially protective IgM autoantibodies. The present observations support a pathophysiological role of PLTP in decreasing the vitamin E content of apolipoprotein B-containing lipoproteins, but not of HDL in plasma of type 2 diabetic patients, contributing to a greater potential for LDL oxidation. Diabetes 53:2633-2639, 2004, The plasma phospholipid transfer protein (PLTP) mediates both net transfer and exchange of phospholipids between lipoproteins (1). Recently, the biological function of PLTP appeared more complex in nature, with its [...]

Published
2004

8. Apparent Km of mitochondria for oxygen computed from Vmax measured in permeabilized muscle fibers is lower in water enriched in oxygen by electrolysis than injection

Author

Zoll,Joffrey, Bouitbir,Jamal, Sirvent,Pascal, Klein,Alexis, Charton,Antoine, Jimenez,Liliana, Péronnet,François R, Geny,Bernard, Richard,Ruddy, Zoll,Joffrey, Bouitbir,Jamal, Sirvent,Pascal, Klein,Alexis, Charton,Antoine, Jimenez,Liliana, Péronnet,François R, Geny,Bernard, and Richard,Ruddy

Abstract

Joffrey Zoll,1 Jamal Bouitbir,1 Pascal Sirvent,2 Alexis Klein,3 Antoine Charton,1,4 Liliana Jimenez,3 François R Péronnet,5 Bernard Geny,1 Ruddy Richard61Physiology Department, Faculty of Medicine and EA3072, Université de Strasbourg, Strasbourg, 2Clermont Université, Université Blaise Pascal, EA 3533, Laboratoire des Adaptations Métaboliques à l’Exercice en Conditions Physiologiques et Pathologiques, Clermont-Ferrand, 3Danone Research, Centre Daniel Carasso, Palaiseau, 4Department of Anesthesia and Critical Care and EA3072, Hôpital de Hautepierre, Université de Strasbourg, France; 5Kinesiology Department, Université de Montréal, Montréal, QC, Canada; 6Department of Sport Medicine and Functional Explorations and INRA UMR 1019, Faculty of Medicine, Université d’Auvergne, Clermont-Ferrand, FranceBackground: It has been suggested that oxygen (O2) diffusion could be favored in water enriched in O2 by a new electrolytic process because of O2 trapping in water superstructures (clathrates), which could reduce the local pressure/content relationships for O2 and facilitate O2 diffusion along PO2 gradients.Materials and methods: Mitochondrial respiration was compared in situ in saponin-skinned fibers isolated from the soleus muscles of Wistar rats, in solution enriched in O2 by injection or the electrolytic process 1) at an O2 concentration decreasing from 240 µmol/L to 10 µmol/L (132 mmHg to 5 mmHg), with glutamate–malate or N, N, N', N'-tetramethyl-p-phenylenediamine dihydrochloride (TMPD)–ascorbate (with antimycin A) as substrates; and 2) at increasing adenosine diphosphate (ADP) concentration with glutamate–malate as substrate.Results: As expected, maximal respiration decreased with O2 concentration and, when compared to glutamate&ndas

Published
2015

9. Worsening of Diet-Induced Atherosclerosis in a New Model of Transgenic Rabbit Expressing the Human Plasma Phospholipid Transfer Protein

Author

Pisoni, Amandine, Meunier, Johann, Athias, Anne, Perrier, Véronique, Villard, Vanessa, Verdier, Jean-Michel, Maurice, Tangui, Masson, David, Deckert, Valérie, Gautier, Thomas, Klein, Alexis, Desrumaux, Catherine, Viglietta, Céline, Pais de Barros, Jean-Paul, Le Guern, Naïg, Grober, Jacques, Labbé, Jérôme, Ménétrier, Franck, Ripoll, Pierre-Jean, Leroux-Coyau, Mathieu, Jolivet, Geneviève, Houdebine, Louis-Marie, Lagrost, Laurent, Pathogénèse et contrôle des infections chroniques (PCCI), Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Cognition, Action, et Plasticité Sensorimotrice [Dijon - U1093] (CAPS), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM), Etude des Civilisations de l'Antiquité (UMR 7044), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Université Marc Bloch - Strasbourg II-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Plateforme Lipidomique [Dijon] (LAP), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Laboratoire d'Excellence : Lipoprotéines et Santé : prévention et Traitement des maladies Inflammatoires et du Cancer (LabEx LipSTIC), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université de Montpellier (UM), Centre de Microscopie Appliqué à la Biologie et à la Médecine, Pharming Group N.V. (Evry), Pharming Group N.V. (Pays-Bas), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL), Biologie du développement et reproduction (BDR), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Centre National de la Recherche Scientifique (CNRS), INRA, unité de Différenciation cellulaire, Services généraux de centre, Biologie du Développement et Reproduction (BDR), Université de Bourgogne, Centre Hospitalier Universitaire de Dijon, Conseil Régional de Bourgogne, Institut National de la Santé et de la Recherche Médicale, Agence Nationale de la Recherche (Atherolip Project), Fondation de France, Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 2 - Sciences et Techniques (UM2)-École pratique des hautes études (EPHE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), IMPEC - LATMOS, Laboratoire Atmosphères, Milieux, Observations Spatiales (LATMOS), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Paris-Sud - Paris 11 (UP11)-École pratique des hautes études (EPHE)-Institut Gustave Roussy (IGR)-Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Régional de Lutte contre le cancer - Centre Georges-François Leclerc (CRLCC - CGFL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Fédération Francophone de la Cancérologie Digestive, FFCD-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Etablissement français du sang [Bourgogne-France-Comté] (EFS [Bourgogne-France-Comté])-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Université de Franche-Comté (UFC)-Université de Montpellier (UM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-IFR100 - Structure fédérative de recherche Santé-STIC-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Institut National de la Recherche Agronomique ( INRA ), and Biologie du Développement et Reproduction ( BDR )

Subjects
Time Factors, Apolipoprotein B, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV]Life Sciences [q-bio], 030204 cardiovascular system & hematology, [ SDV.BA ] Life Sciences [q-bio]/Animal biology, Animals, Genetically Modified, Cholesterol, Dietary, chemistry.chemical_compound, 0302 clinical medicine, Peptide Elongation Factor 1, Phospholipid transfer protein, Phospholipid Transfer Proteins, Promoter Regions, Genetic, [SDV.BDD]Life Sciences [q-bio]/Development Biology, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, 0303 health sciences, Lagomorpha, [SDV.BA]Life Sciences [q-bio]/Animal biology, Recombinant Proteins, [ SDV.BDLR ] Life Sciences [q-bio]/Reproductive Biology, lipids (amino acids, peptides, and proteins), Rabbits, Cardiology and Cardiovascular Medicine, medicine.medical_specialty, Transgene, Hypercholesterolemia, Aortic Diseases, Biology, Transfection, 03 medical and health sciences, Complementary DNA, Internal medicine, [SDV.BDD] Life Sciences [q-bio]/Development Biology, medicine, Animals, Humans, [ SDV.BDD ] Life Sciences [q-bio]/Development Biology, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, 030304 developmental biology, Apolipoproteins B, Cholesterol, Cholesterol, HDL, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Metabolism, biology.organism_classification, HCT116 Cells, lipoproteins, Disease Models, Animal, Endocrinology, chemistry, biology.protein, atherosclerosis, transgenic models, metabolism, Biomarkers, Lipoprotein
Abstract

Objective— Plasma phospholipid transfer protein (PLTP) is involved in intravascular lipoprotein metabolism. PLTP is known to act through 2 main mechanisms: by remodeling high-density lipoproteins (HDL) and by increasing apolipoprotein (apo) B–containing lipoproteins. The aim of this study was to generate a new model of human PLTP transgenic (HuPLTPTg) rabbit and to determine whether PLTP expression modulates atherosclerosis in this species that, unlike humans and mice, displays naturally very low PLTP activity. Methods and Results— In HuPLTPTg rabbits, the human PLTP cDNA was placed under the control of the human eF1-α gene promoter, resulting in a widespread tissue expression pattern and in increased plasma PLTP. The HuPLTPTg rabbits showed a significant increase in the cholesterol content of the plasma apoB-containing lipoprotein fractions, with a more severe trait when animals were fed a cholesterol-rich diet. In contrast, HDL cholesterol level was not modified in HuPLTPTg rabbits. Formation of aortic fatty streaks was increased in hypercholesterolemic HuPLTPTg animals as compared with nontransgenic littermates. Conclusion— Human PLTP expression in HuPLTPTg rabbit worsens atherosclerosis as a result of increased levels of atherogenic apoB-containing lipoproteins but not of alterations in their antioxidative protection or in cholesterol content of plasma HDL.

Published
2011

12. Effect of administration of water enriched in O2 by injection or electrolysis on transcutaneous oxygen pressure in anesthetized pigs

Author

Charton,Antoine, Péronnet,François, Doutreleau,Stephane, Lonsdorfer,Evelyne, Klein,Alexis, Jimenez,Liliana, Geny,Bernard, Diemunsch,Pierre, Richard,Ruddy, Charton,Antoine, Péronnet,François, Doutreleau,Stephane, Lonsdorfer,Evelyne, Klein,Alexis, Jimenez,Liliana, Geny,Bernard, Diemunsch,Pierre, and Richard,Ruddy

Abstract

Antoine Charton,1 François Péronnet,2 Stephane Doutreleau,3 Evelyne Lonsdorfer,3 Alexis Klein,4 Liliana Jimenez,4 Bernard Geny,3 Pierre Diemunsch,1 Ruddy Richard5 1Department of Anesthesia and Critical Care, and EA 3072, Hôpital de Hautepierre; University of Strasbourg, Strasbourg, France; 2Department of Kinesiology, Université de Montréal, Montreal, QC, Canada; 3CHRU of Strasbourg, Physiology and Functional Explorations Department, New Civil Hospital, Strasbourg, France and University of Strasbourg, Faculty of Medicine, Physiology Department, Strasbourg, France; 4Danone Research, Palaiseau, France; 5Department of Sport Medicine and Functional Explorations, CHU Clermont-Ferrand and INRA UMR 1019, CRNH-Auvergne, Clermont-Ferrand, France Background: Oral administration of oxygenated water has been shown to improve blood oxygenation and could be an alternate way for oxygen (O2) supply. In this experiment, tissue oxygenation was compared in anesthetized pigs receiving a placebo or water enriched in O2 by injection or a new electrolytic process. Methods: Forty-two pigs randomized in three groups received either mineral water as placebo or water enriched in O2 by injection or the electrolytic process (10 mL/kg in the stomach). Hemodynamic parameters, partial pressure of oxygen in the arterial blood (PaO2), skin blood flow, and tissue oxygenation (transcutaneous oxygen pressure, or TcPO2) were monitored during 90 minutes of general anesthesia. Absorption and tissue distribution of the three waters administered were assessed using dilution of deuterium oxide. Results: Mean arterial pressure, heart rate, PaO2, arteriovenous oxygen difference, and water absorption from the gut were not significantly different among the three groups. The deuterium to protium ratio was also similar in the plasma, skin, and muscle at the end of the protocol. Skin blood flow decreased in the three groups. TcPO2 slowly decreased over the last 6

Published
2014

13. Vitamin E transport in enterocytes

Author

Reboul, Emmanuelle, Klein, Alexis, Bietrix, Florence, Gleize, Béatrice, Malezet-Desmoulins, Christiane, Schneider, Martina, Margotat, Alain, Lagrost, Laurent, Collet, Xavier, Borel, Patrick, Nutrition humaine et lipides : Biodisponibilité, métabolisme et régulation, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Recherche Agronomique (INRA)-Université de Provence - Aix-Marseille 1-IFR125-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire des Lipoprotéines humaines et interactions vasculaires, Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (CPTP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH: Cell Differentiation, MESH: Mice, Transgenic, MESH: Biological Transport, MESH: Binding, Competitive, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Transgenic, Absorption, MESH: Dose-Response Relationship, Drug, Dose-Response Relationship, Mice, MESH: Enterocytes, MESH: Cholesterol, Competitive, Animals, Humans, MESH: Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Antigens, MESH: Mice, Micelles, MESH: Humans, MESH: Antigens, CD36, MESH: Absorption, MESH: Micelles, Cell Differentiation, Epithelial Cells, Biological Transport, Binding, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Lipids, MESH: Lipids, Intestines, Cholesterol, Enterocytes, MESH: Epithelial Cells, MESH: Caco-2 Cells, Drug, Caco-2 Cells, CD36, MESH: Intestines
Abstract

Although cellular uptake of vitamin E was initially described as a passive process, recent studies in the liver and brain have shown that SR-BI (scavenger receptor class B type I) is involved in this phenomenon. As SR-BI is expressed at high levels in the intestine, the present study addressed the involvement of SR-BI in vitamin E trafficking across enterocytes. Apical uptake and efflux of the main dietary forms of vitamin E were examined using Caco-2 TC-7 cell monolayers as a model of human intestinal epithelium. (R,R,R)-gamma-tocopherol bioavailability was compared between wild-type mice and mice overexpressing SR-BI in the intestine. The effect of vitamin E on enterocyte SR-BI mRNA levels was measured by real-time quantitative reverse transcription-PCR. Concentration-dependent curves for vitamin E uptake were similar for (R,R,R)-alpha-, (R,R,R)-gamma-, and dl-alpha-tocopherol. (R,R,R)-alpha-tocopherol transport was dependent on incubation temperature, with a 60% reduction in absorption at 4 degrees C compared with 37 degrees C (p < 0.05). Vitamin E flux in enterocytes was directed from the apical to the basal side, with a relative 10-fold reduction in the transfer process when measured in the opposite direction (p < 0.05). Co-incubation with cholesterol, gamma-tocopherol, or lutein significantly impaired alpha-tocopherol absorption. Anti-human SR-BI antibodies and BLT1 (a chemical inhibitor of lipid transport via SR-BI) blocked up to 80% of vitamin E uptake and up to 30% of apical vitamin E efflux (p < 0.05), and similar results were obtained for (R,R,R)-gamma-tocopherol. SR-BI mRNA levels were not significantly modified after a 24-h incubation of Caco-2 cells with vitamin E. Finally, (R,R,R)-gamma-tocopherol bioavailability was 2.7-fold higher in mice overexpressing SR-BI than in wild-type mice (p < 0.05). The present data show for the first time that vitamin E intestinal absorption is, at least in part, mediated by SR-BI.

Published
2006

14. Scavenger receptor class B type I (SR-BI) is involved in vitamin E transport across the enterocyte. : Vitamin E transport in enterocytes

Author

Reboul, Emmanuelle, Klein, Alexis, Bietrix, Florence, Gieize, Beatrice, Malezet-Desmoulins, Christiane, Schneider, Martina, Margotat, Alain, Lagrost, Laurent, Collet, Xavier, and Borel, Patrick

Subjects
SDV:BC, Endocrinology and metabolism, Endocrinologie et métabolisme, SDV:BBM, Absorption, Animals, Antigens, CD36, Binding, Competitive, Biological Transport, Caco-2 Cells, Cell Differentiation, Cholesterol, Dose-Response Relationship, Drug, Enterocytes, Epithelial Cells, Humans, Intestines, Lipids, Mice, Transgenic, Micelles
Abstract

Although cellular uptake of vitamin E was initially described as a passive process, recent studies in the liver and brain have shown that SR-BI (scavenger receptor class B type I) is involved in this phenomenon. As SR-BI is expressed at high levels in the intestine, the present study addressed the involvement of SR-BI in vitamin E trafficking across enterocytes. Apical uptake and efflux of the main dietary forms of vitamin E were examined using Caco-2 TC-7 cell monolayers as a model of human intestinal epithelium. (R,R,R)-gamma-tocopherol bioavailability was compared between wild-type mice and mice overexpressing SR-BI in the intestine. The effect of vitamin E on enterocyte SR-BI mRNA levels was measured by real-time quantitative reverse transcription-PCR. Concentration-dependent curves for vitamin E uptake were similar for (R,R,R)-alpha-, (R,R,R)-gamma-, and dl-alpha-tocopherol. (R,R,R)-alpha-tocopherol transport was dependent on incubation temperature, with a 60% reduction in absorption at 4 degrees C compared with 37 degrees C (p < 0.05). Vitamin E flux in enterocytes was directed from the apical to the basal side, with a relative 10-fold reduction in the transfer process when measured in the opposite direction (p < 0.05). Co-incubation with cholesterol, gamma-tocopherol, or lutein significantly impaired alpha-tocopherol absorption. Anti-human SR-BI antibodies and BLT1 (a chemical inhibitor of lipid transport via SR-BI) blocked up to 80% of vitamin E uptake and up to 30% of apical vitamin E efflux (p < 0.05), and similar results were obtained for (R,R,R)-gamma-tocopherol. SR-BI mRNA levels were not significantly modified after a 24-h incubation of Caco-2 cells with vitamin E. Finally, (R,R,R)-gamma-tocopherol bioavailability was 2.7-fold higher in mice overexpressing SR-BI than in wild-type mice (p < 0.05). The present data show for the first time that vitamin E intestinal absorption is, at least in part, mediated by SR-BI.

Published
2006

18. From State to Process: Defining Hydration

Author

Perrier, Erica T., primary, Armstrong, Lawrence E., additional, Daudon, Michel, additional, Kavouras, Stavros, additional, Lafontan, Max, additional, Lang, Florian, additional, Péronnet, François, additional, Stookey, Jodi D., additional, Tack, Ivan, additional, and Klein, Alexis, additional

Published
2014
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22. Worsening of Diet-Induced Atherosclerosis in a New Model of Transgenic Rabbit Expressing the Human Plasma Phospholipid Transfer Protein

Author

Masson, David, primary, Deckert, Valérie, additional, Gautier, Thomas, additional, Klein, Alexis, additional, Desrumaux, Catherine, additional, Viglietta, Céline, additional, Pais de Barros, Jean-Paul, additional, Le Guern, Naig, additional, Grober, Jacques, additional, Labbé, Jérôme, additional, Ménétrier, Franck, additional, Ripoll, Pierre-Jean, additional, Leroux-Coyau, Mathieu, additional, Jolivet, Geneviève, additional, Houdebine, Louis-Marie, additional, and Lagrost, Laurent, additional

Published
2011
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23. Plasma Phospholipid Transfer Protein Deficiency in Mice Is Associated With a Reduced Thrombotic Response to Acute Intravascular Oxidative Stress

Author

Desrumaux, Catherine, primary, Deckert, Valérie, additional, Lemaire-Ewing, Stéphanie, additional, Mossiat, Claude, additional, Athias, Anne, additional, Vandroux, David, additional, Dumont, Laure, additional, Monier, Serge, additional, Pais de Barros, Jean-Paul, additional, Klein, Alexis, additional, De Maistre, Emmanuel, additional, Blache, Denis, additional, Beley, Alain, additional, Marie, Christine, additional, Garnier, Philippe, additional, and Lagrost, Laurent, additional

Published
2010
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24. Effect of Plasma Phospholipid Transfer Protein Deficiency on Lethal Endotoxemia in Mice

Author

Gautier, Thomas, primary, Klein, Alexis, additional, Deckert, Valérie, additional, Desrumaux, Catherine, additional, Ogier, Nicolas, additional, Sberna, Anne-Laure, additional, Paul, Catherine, additional, Le Guern, Naig, additional, Athias, Anne, additional, Montange, Thomas, additional, Monier, Serge, additional, Piard, Françoise, additional, Jiang, Xian-Cheng, additional, Masson, David, additional, and Lagrost, Laurent, additional

Published
2008
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27. α-Tocopherol Modulates Phosphatidylserine Externalization in Erythrocytes

Author

Klein, Alexis, primary, Deckert, Valérie, additional, Schneider, Martina, additional, Dutrillaux, Fabienne, additional, Hammann, Arlette, additional, Athias, Anne, additional, Le Guern, Naig, additional, Pais de Barros, Jean-Paul, additional, Desrumaux, Catherine, additional, Masson, David, additional, Jiang, Xian-Cheng, additional, and Lagrost, Laurent, additional

Published
2006
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30. Hydration biomarkers in free-living adults with different levels of habitual fluid consumption.

Author

Perrier, Erica, Vergne, Sébastien, Klein, Alexis, Poupin, Marie, Rondeau, Pascale, Le Bellego, Laurent, Armstrong, Lawrence E., Lang, Florian, Stookey, Jodi, and Tack, Ivan

Subjects
KIDNEY disease risk factors, ANALYSIS of variance, BIOMARKERS, BLOOD testing, COMPARATIVE studies, CREATININE, DRINKING (Physiology), HOMEOSTASIS, HYDROCORTISONE, NONPARAMETRIC statistics, NUTRITIONAL assessment, QUESTIONNAIRES, RESEARCH funding, STATISTICAL hypothesis testing, STATISTICS, URINALYSIS, VASOPRESSIN, WATER-electrolyte balance (Physiology), WHITE people, DATA analysis, STATISTICAL significance, BODY mass index, REPEATED measures design, CROSS-sectional method, OSMOLAR concentration, DATA analysis software, DIARY (Literary form), DESCRIPTIVE statistics
Abstract

Little is known about the impact of habitual fluid intake on physiology. Specifically, biomarkers of hydration status and body water regulation have not been adequately explored in adults who consume different fluid volumes in everyday conditions, without prolonged exercise or environmental exposure. The purpose of the present study was to compare adults with habitually different fluid intakes with respect to biomarkers implicated in the assessment of hydration status, the regulation of total body water and the risk of kidney pathologies. In the present cross-sectional study, seventy-one adults (thirty-two men, thirty-nine women, age 25–40 years) were classified according to daily fluid intake: thirty-nine low drinkers (LD; ≤ 1·2 litres/d) and thirty-two high drinkers (HD; 2–4 litres/d). During four consecutive days, urinary parameters (first morning urine (FMU) on day 1 and subsequent 24 h urine (24hU) collections), blood parameters, and food and beverage intake were assessed. ANOVA and non-parametric comparisons revealed significant differences between the LD and HD groups in 24hU volume (1·0 (se 0·1) v. 2·4 (se 0·1) litres), specific gravity (median 1·023 v. 1·010), osmolality (767 (se 27) v. 371 (se 33) mOsm/kg) and colour (3·1 (se 0·2) v. 1·8 (se 0·2)). Similarly, in the FMU, the LD group produced a smaller amount of more concentrated urine. Plasma cortisol, creatinine and arginine vasopressin concentrations were significantly higher among the LD. Plasma osmolality was similar between the groups, suggesting physiological adaptations to preserve plasma osmolality despite low fluid intake. The long-term impact of adaptations to preserve plasma osmolality must be examined, particularly in the context of renal health. [ABSTRACT FROM PUBLISHER]

Published
2013
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31. Influence of progressive fluid restriction on mood and physiological markers of dehydration in women.

Author

Pross, Nathalie, Demazières, Agnès, Girard, Nicolas, Barnouin, Romain, Santoro, Francine, Chevillotte, Emmanuel, Klein, Alexis, and Le Bellego, Laurent

Subjects
AFFECT (Psychology), ANALYSIS of covariance, ANALYSIS of variance, BLOOD plasma, COGNITION, COLOR, CONFIDENCE intervals, CROSSOVER trials, DEHYDRATION, SPECIFIC gravity, PROBABILITY theory, QUESTIONNAIRES, RESEARCH funding, SALIVA, SCALES (Weighing instruments), STATISTICAL hypothesis testing, THIRST, URINE, EVALUATION research, OSMOLAR concentration, DISEASE complications
Abstract

The present study evaluated, using a well-controlled dehydration protocol, the effects of 24 h fluid deprivation (FD) on selected mood and physiological parameters. In the present cross-over study, twenty healthy women (age 25 (se 0·78) years) participated in two randomised sessions: FD-induced dehydration v. a fully hydrated control condition. In the FD period, the last water intake was between 18.00 and 19.00 hours and no beverages were allowed until 18.00 hours on the next day (23–24 h). Water intake was only permitted at fixed periods during the control condition. Physiological parameters in the urine, blood and saliva (osmolality) as well as mood and sensations (headache and thirst) were compared across the experimental conditions. Safety was monitored throughout the study. The FD protocol was effective as indicated by a significant reduction in urine output. No clinical abnormalities of biological parameters or vital signs were observed, although heart rate was increased by FD. Increased urine specific gravity, darker urine colour and increased thirst were early markers of dehydration. Interestingly, dehydration also induced a significant increase in saliva osmolality at the end of the 24 h FD period but plasma osmolality remained unchanged. The significant effects of FD on mood included decreased alertness and increased sleepiness, fatigue and confusion. The most consistent effects of mild dehydration on mood are on sleep/wake parameters. Urine specific gravity appears to be the best physiological measure of hydration status in subjects with a normal level of activity; saliva osmolality is another reliable and non-invasive method for assessing hydration status. [ABSTRACT FROM PUBLISHER]

Published
2013
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32. Legislation Editor's Note.

Author

Klein, Alexis Michelle

Subjects
*LEGISLATIVE bills, *LEGISLATION
Abstract

An introduction to the journal is presented in which the editor discusses an article on the background and legal implications of important California legislation enacted during the first half of the 2009-2010, California Legislature's responses to the troubled condition of the economy, and the posthumous application of the attorney-client privilege.

Published
2010

33. Alpha-tocopherol modulates phosphatidylserine externalization in erythrocytes: relevance in phospholipid transfer protein-deficient mice.

Author

Klein A, Deckert V, Schneider M, Dutrillaux F, Hammann A, Athias A, Le Guern N, Pais de Barros JP, Desrumaux C, Masson D, Jiang XC, and Lagrost L

Subjects
Animals, Biomarkers blood, Blood Coagulation physiology, Cell Separation, Erythrocytes physiology, Fibrin Fibrinogen Degradation Products metabolism, Homozygote, Mice, Mice, Knockout, Oxidation-Reduction, Phenotype, Whole Blood Coagulation Time, Erythrocyte Membrane metabolism, Phosphatidylserines blood, Phospholipid Transfer Proteins deficiency, alpha-Tocopherol pharmacology
Abstract

Objective: The aim of the present study was to assess the effect of alpha-tocopherol, the main vitamin E isomer on phosphatidylserine (PS) exposure at the surface of circulating erythrocytes, and to determine consequences on erythrocyte properties., Methods and Results: In vitro alpha-tocopherol enrichment of isolated erythrocytes significantly decreased PS externalization as assessed by lower Annexin V-fluorescein isothiocyanate labeling. Plasma phospholipid transfer protein (PLTP) transfers vitamin E, and both alpha- and gamma-tocopherol accumulated in circulating erythrocytes from PLTP-deficient homozygous (PLTP-/-) mice as compared with wild-type mice. In agreement with in vitro studies, vitamin E-enriched erythrocytes from PLTP-/- mice displayed fewer externalized PS molecules than wild-type controls (-64%, P<0.05). The perturbation of phospholipid membrane asymmetry from PLTP-/- erythrocytes was accompanied by impairment of their procoagulant properties, with a 20% increase in clotting time as compared with wild-type controls (P<0.05). Less pronounced, however still significant, changes were observed in alpha-tocopherol content, procoagulant properties, and PS externalization in erythrocytes of PLTP-deficient heterozygotes. Finally, whole blood coagulation and circulating level of D-dimer, which reflects increased thrombus formation in vivo, were significantly decreased in PLTP-/- mice compared with wild-type mice., Conclusions: Vitamin E modifies PS externalization in circulating erythrocytes, thus modulating in vivo their PS-dependent procoagulant properties.

Published
2006
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