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Pakistan is the fifth most populous country in the world. Its ecological environment is facing numerous stresses such as climate change, rapid urbanization, natural disasters, and a decline in air quality. Thus, the scientific understanding of spatial and temporal changes in Pakistan's ecological environment is crucial for formulating an informed strategy for regional sustainability. This study used the Google Earth Engine platform and the Remote Sensing Ecological Index (RSEI) to investigate ecological vulnerability in three provinces of Pakistan for 1990, 2000, 2010, and 2020. Landsat 5 and Landsat 8 datasets are used to construct RSEI indicators and Principal Component Analysis (PCA) is adopted to objectively compute RSEI for the past three decades. The results indicated that (1) the ecological vulnerability of the Punjab province exhibited a slightly improved trend from 1990 to 2020 with overall dominance of ‘moderate’ level vulnerability in all four years; (2) The Sindh province has exhibited a declining trend of the ecological environment with ‘poor’ ecological vulnerability contributing to 28.6% of the total area in 2020 as compared to 1.04% of the total area in 1990; and (3) The ecological environment of Balochistan province has shown resilience to some extent during 1900–2010 with the dominance of a ‘moderate’ level of ecological vulnerability. However, a decline is observed between 2010 and 2020. These research results can provide support for the Punjab, Sindh, and Balochistan provinces in achieving sustainable development while conserving the regional ecological environment.

Abstract The coexistence of three sexual phenotypes (male, female and bisexual) in a single species, ‘trioecy’, is rarely found in diploid organisms such as flowering plants and invertebrates. However, trioecy in haploid organisms has only recently been reported in a green algal species, Pleodorina starrii. Here, we generated whole-genome data of the three sex phenotypes of P. starrii to reveal a reorganization of the ancestral sex-determining regions (SDRs) in the sex chromosomes: the male and bisexual phenotypes had the same “male SDR” with paralogous gene expansions of the male-determining gene MID, whereas the female phenotype had a “female SDR” with transposition of the female-specific gene FUS1 to autosomal regions. Although the male and bisexual sex phenotypes had the identical male SDR and harbored autosomal FUS1, MID and FUS1 expression during sexual reproduction differed between them. Thus, the coexistence of three sex phenotypes in P. starrii is possible.

This study analyzed radiation dose data to observe the annual decline in ambient radiation doses and assess the factors contributing to fluctuations in reconstructed areas of the Fukushima prefecture. Utilizing a novel mobile monitoring system installed on a community minibus, the study employed a cost-effective sensor, namely, Pocket Geiger which was integrated with a microcontroller and telecommunication system for data transfer, access, visualization, and accumulation. The study area included the region between Okuma and Tomioka towns. The ambient dose rate recorded along the minibus route was depicted on a map, averaged within a 1 × 1 km mesh created with the Quantum Geographic Information System. To ensure accuracy, the shielding factor of the minibus material is determined to adjust the dose readings. A significant decrease (p < 0.001) in the radiation dose ranges from 2022 to 2023 was observed. The land use classification by the Advanced Land Observation Satellite revealed an ecological half-life ranging from 2.41 years to 1 year, suggesting a rapid radiation decay across all land types. This underscores the close connection between radiation attenuation and environmental factors, as well as decontamination efforts across diverse land categories.

Background: The ecological vulnerability assessment (EVA) is employed as a tool for integrated evaluation of the ecosystem with the help of indicators belonging to natural, social, and economy systems. The unavailability of suitable datasets has repeatedly been reported as an obstacle to conducting EVA on large spatial and temporal scales. The progress in remote sensing (RS) technology has aided the development of ecological vulnerability indicators (EVIs) from RS datasets. Scope and approach: This manuscript presents a comprehensive review of 80 peer-reviewed publications which uses RS for EVA. In this regard, the paper is divided into five main sections: First, the background and rationale of conducting this review is presented in the introduction. Second, the methodology for selection and interpretation of selected literature is discussed. Then, the results section presents findings from various dimensions: general trend of literature, spatial or temporal scale of EVA, analysis of targets systems, RS and Non-RS derived EVIs, sensors used for acquisition of RS data, conceptual models, weights determining mechanisms, and software. Next, multiple gaps in the body of knowledge are identified and general recommendations are formulated for future researchers. Finally, the link of EVA with sustainable development is highlighted in the conclusion section. Key findings: The overall trend shows that both the quantity and quality of research regarding EVA has increased over the past decades. It is observed that large number of studies report EVA on both temporal and spatial scale. Further, the results reveal 52 EVIs are derived from RS data and 101 EVIs are derived from non-RS data. The Landsat datasets are found most repeatedly used in EVA research. The remote sensing ecological index (RSEI) and principal component analysis (PCA) are prominent conceptual models and weighting mechanisms. Finally, ArcGIS software is most widely reported software in the EVA literature.

Oral microbiota may be associated with serious local or systemic medical conditions resulting from chemotherapy. This study was conducted to evaluate the changes in the oral microbiota following the initiation of chemotherapy in patients with hematopoietic malignancies and to identify the characteristics of the oral microbiota associated with oral mucositis. Oral samples were collected from 57 patients with hematopoietic malignancies at 2 time points: before the start of chemotherapy and 8 to 20 days after the start of chemotherapy, when chemotherapy-induced oral mucositis often occurs, and 16S rRNA metagenomic analyses were performed. Comparative and linear discriminant analysis effect size (LEfSe) analyses were used to determine the characteristic bacterial groups before and after the initiation of chemotherapy and in those who developed oral mucositis. The alpha and beta diversities of oral microbiota before and after the initiation of chemotherapy differed significantly (operational taxonomic unit index, P

Abstract Germ-soma differentiation evolved independently in many eukaryotic lineages and contributed to complex multicellular organizations. However, the molecular genetic bases of such convergent evolution remain unresolved. Two multicellular volvocine green algae, Volvox and Astrephomene, exhibit convergent evolution of germ-soma differentiation. The complete genome sequence is now available for Volvox, while genome information is scarce for Astrephomene. Here, we generated the de novo whole genome sequence of Astrephomene gubernaculifera and conducted RNA-seq analysis of isolated somatic and reproductive cells. In Volvox, tandem duplication and neofunctionalization of the ancestral transcription factor gene (RLS1/rlsD) might have led to the evolution of regA, the master regulator for Volvox germ-soma differentiation. However, our genome data demonstrated that Astrephomene has not undergone tandem duplication of the RLS1/rlsD homolog or acquisition of a regA-like gene. Our RNA-seq analysis revealed the downregulation of photosynthetic and anabolic gene expression in Astrephomene somatic cells, as in Volvox. Among genes with high expression in somatic cells of Astrephomene, we identified three genes encoding putative transcription factors, which may regulate somatic cell differentiation. Thus, the convergent evolution of germ-soma differentiation in the volvocine algae may have occurred by the acquisition of different regulatory circuits that generate a similar division of labor.

It is important that both static and dynamic information is efficiently used to create a suitable tourism plan. However, collecting, accumulating and managing dynamic information can cost tourists time, money and energy. In the present study, an original tourism support system was designed and developed with the purpose of reducing the burden of tourists who are unfamiliar with the urban tourist destinations in particular. An original tourism planning support system and web-geographic information systems (Web-GIS) were integrated into a single system, and two original functions were implemented. The system was operated targeting Osaka City, Japan, for a period of one month. The information concerning 529 sightseeing spots was collected from tourism-related web media and then saved to the database of the system beforehand. During the operation period, a cumulative total of 160 users accessed the system. Based on the questionnaire survey results, most of the main functions were highly rated regardless of users’ travel experiences to the operation target area. Additionally, based on the access log analysis results, it was clear that the use of the system was in line with the purpose of the present study, as the number of visits to the page for the main functions were high.

Although mechanisms that activate organogenesis in plants are well established, much less is known about the subsequent fine-tuning of cell proliferation, which is crucial for creating properly structured and sized organs. Here we show, through analysis of temperature-dependent fasciation (TDF) mutants of Arabidopsis, root redifferentiation defective 1 (rrd1), rrd2, and root initiation defective 4 (rid4), that mitochondrial RNA processing is required for limiting cell division during early lateral root (LR) organogenesis. These mutants formed abnormally broadened (i.e. fasciated) LRs under high-temperature conditions due to extra cell division. All TDF proteins localized to mitochondria, where they were found to participate in RNA processing: RRD1 in mRNA deadenylation, and RRD2 and RID4 in mRNA editing. Further analysis suggested that LR fasciation in the TDF mutants is triggered by reactive oxygen species generation caused by defective mitochondrial respiration. Our findings provide novel clues for the physiological significance of mitochondrial activities in plant organogenesis.

Summary: Although many oral ulcers have similar clinical appearances, their etiologies can range from reactive to neoplastic to oral manifestations of dermatological diseases. In patients with an HIV infection, fungal diseases may cause ulceration in the oral cavity; however, there have been few studies of oral ulcerative lesions associated with Candida in patients without an HIV infection. Nevertheless, we encountered chronic oral ulcer associated with Candida among our frequent outpatients without an HIV infection. The present article reviews the causes of oral ulcers, focusing on Candida as a protractive factor for chronic oral ulcers, and it is recommended that Candida involvement be considered in diagnosis of a certain chronic oral ulcer, that remains of unknown origin even if some examinations have been performed. Keywords: Oral candidiasis, Secondary candidiasis, Chronic oral ulcer, Candida-associated lesions

In tourist areas, it is necessary to prepare a method that supports tourists’ activities by providing information concerning disaster support facilities during normal times, in addition to sightseeing spots and tourism-related facilities, because there is a risk of confusion during disasters, as tourists are not aware of the locations of disaster support facilities. The present study aims to develop a navigation system that supports the activities of users during both normal times and disasters by integrating augmented reality (AR) and web geographic information systems (Web-GISs), as well as by using pictograms. The system can not only effectively provide users with information concerning sightseeing spots and tourism-related facilities but also information concerning disaster support facilities. The system was operated over a period of 6 weeks in Chofu City, Tokyo Metropolis, Japan. Based on the results of a questionnaire survey for 60 users, the system was highly evaluated for its originality in terms of displays and functions using pictograms, navigation using AR, and obtaining information during disasters. Additionally, based on the results of access log analysis, the system was continuously utilized by users during the operation period. Therefore, by continuously operating the system, it can be expected that users will further utilize each function of the system.

The present study aimed to describe the utilization of Information and Communication Technology (ICT) as a digital infrastructure concerning disaster countermeasures in Japan. Specifically, the study introduced development cases of the systems integrating social media and Geographic Information Systems (GIS), and presented the utilization potential as a digital infrastructure. Additionally, taking up Twitter as a familiar digital infrastructure, the study also presented its utilization potential based on the case of the heavy-rain disaster in Western Japan in 2018. As a result, due to the close relationship between the reality and virtual spaces, the issue is how to make the information circulating in the virtual space efficiently and effectively aid in the rescue and support activities in the reality space. The above systems are effective in order to solve such an issue, because these can efficiently consolidate the essential information on the digital map of Web-GIS. Additionally, it is necessary to set rules for the utilization of social media, and sift through information and share only the necessary information to the affected local governments and those involved in the rescue and support activities. Furthermore, various information communication methods including verbal calls in addition to ICT are necessary especially for people who are vulnerable to information.

The present study aimed to design, develop, operate and evaluate a sightseeing spot recommendation system that can efficiently and usefully support tourists while considering their visiting frequencies. This system was developed by integrating social networking services (SNSs), Web-geographic information systems (GIS) and recommendation systems. The system recommends sightseeing spots to users with different visiting frequencies, adopting two recommendation methods (knowledge-based recommendation and collaborative recommendation methods). Additionally, the system was operated for six weeks in Kamakura City, Kanagawa Prefecture, Japan, and the total number of users was 61. Based on the results of the web questionnaire survey, the usefulness of the system when sightseeing was high, and the recommendation function of sightseeing spots, which is an original function, received mainly good ratings. From the results of the access analysis of users’ log data, the total number of sessions in this system was 329, 77% used mobile devices, and smartphones were used most frequently. Therefore, it is evident that the system was used by different types of devices just as it was designed for, and that the system was used according to the purpose of the present study, which is to support the sightseeing activities of users.

Volvox is a very interesting oogamous organism that exhibits various types of sexuality and/or sexual spheroids depending upon species or strains. However, molecular bases of such sexual reproduction characteristics have not been studied in this genus. In the model species V. carteri, an ortholog of the minus mating type-determining or minus dominance gene (MID) of isogamous Chlamydomonas reinhardtii is male-specific and determines the sperm formation. Male and female genders are genetically determined (heterothallism) in V. carteri, whereas in several other species of Volvox both male and female gametes (sperm and eggs) are formed within the same clonal culture (homothallism). To resolve the molecular basis of the evolution of Volvox species with monoecious spheroids, we here describe a MID ortholog in the homothallic species V. africanus that produces both monoecious and male spheroids within a single clonal culture. Comparison of synonymous and nonsynonymous nucleotide substitutions in MID genes between V. africanus and heterothallic volvocacean species suggests that the MID gene of V. africanus evolved under the same degree of functional constraint as those of the heterothallic species. Based on semi quantitative reverse transcription polymerase chain reaction analyses using the asexual, male and monoecious spheroids isolated from a sexually induced V. africanus culture, the MID mRNA level was significantly upregulated in the male spheroids, but suppressed in the monoecious spheroids. These results suggest that the monoecious spheroid-specific down regulation of gene expression of the MID homolog correlates with the formation of both eggs and sperm in the same spheroid in V. africanus.

Though tourists can search for necessary information on the internet while sightseeing, it takes effort and is inconvenient to obtain available information related to specific sightseeing spots among the copious amount of information online. Targeting urban tourist areas in Japan, the present study aims to develop a system that can provide guidance and information concerning sightseeing spots by integrating location-based augmented reality (AR) and object-recognition AR and by using pictograms. The system enables users to efficiently obtain the directions to sightseeing spots and nearby facilities within urban tourist areas and sightseeing spot information. Additionally, the city of Chofu in the metropolis of Tokyo was selected as the operation target area. The operation of the system was conducted for 1 month, targeting those inside and outside the operation target area, and a web questionnaire survey was conducted with a total number of 50 users. From the evaluation results of the web questionnaire survey, the usefulness of the original functions of integrating location-based AR and object-recognition AR and by using pictograms, as well as of the entire system, was analyzed. From the results of the access analysis of users’ log data, it is expected that users will further utilize each function. Additionally, it is evident that location-based AR was used more often than was object-recognition AR.

Japan is exposed to several natural phenomena; the damages caused by earthquakes were enormous in particular. An emergency shelter is a place for people to temporarily live when they cannot remain in their previous homes, and it is necessary for each community to, respectively, allocate such facilities in Japan. There are the districts that are short of such facilities, especially in rural and suburban areas, because emergency shelters mainly concentrate near large-scale stations and city centres in Japan. Against these backdrops, using geographic information systems (GIS), an applied statistical method and public open data related to population and emergency shelters, the present research aims to quantitatively conduct a suitability analysis for the emergency shelters allocation after an earthquake in Japan. Based on the results, the present research grasps the districts that are short of emergency shelters, and visually shows the places where such facilities should be newly established on the digital map of GIS. Additionally, the assessment method is reproducible in the spatial and temporal dimension. It is necessary to create an original data related to emergency shelters to raise the reliability of the results, as the present research has the limitation of data availability.

Volvox sect. Volvox is characterized by having unique morphological characteristics, such as thick cytoplasmic bridges between adult somatic cells in the spheroids and spiny zygote walls. Species of this section are found from various freshwater habitats. Recently, three species of Volvox sect. Volvox originating from rice paddies and a marsh were studied taxonomically based on molecular and morphological data of cultured materials. However, taxonomic studies have not been performed on cultured materials of this section originating from large lake water bodies. We studied a new morphological type of Volvox sect. Volvox ("Volvox sp. Sagami"), using cultured materials originating from two large lakes and a pond in Japan. Volvox sp. Sagami produced monoecious sexual spheroids and may represent a new morphological species; it could be clearly distinguished from all previously described monoecious species of Volvox sect. Volvox by its small number of eggs or zygotes (5-25) in sexual spheroids, with short acute spines (up to 3 μm long) on the zygote walls and elongated anterior somatic cells in asexual spheroids. Based on sequences of internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (rDNA; ITS-1, 5.8S rDNA and ITS-2) and plastid genes, however, the Volvox sp. Sagami lineage and its sister lineage (the monoecious species V. ferrisii) showed very small genetic differences, which correspond to the variation within a single biological species in other volvocalean algae. Since V. ferrisii was different from Volvox sp. Sagami, by having approximately 100-200 zygotes in the sexual spheroids and long spines (6-8.5 μm long) on the zygote walls, as well as growing in Japanese rice paddies, these two morphologically distinct lineages might have diverged rapidly in the two different freshwater habitats. In addition, the swimming velocity during phototaxis of Volvox sp. Sagami spheroids originating from large lakes was significantly higher than that of V. ferrisii originating from rice paddies, suggesting adaptation of Volvox sp. Sagami to large water bodies.

The present study aimed to design, develop, operate, and evaluate a recommendation system for meeting places targeting groups of two or more people during events. The system was designed and developed by integrating an accessibility database, as well as a recommendation system, and linking with Google Maps and social networking services (SNSs, Twitter and LINE). Additionally, the system was operated for 5 weeks with people mainly in the Tokyo metropolitan area, with Japan as the target, and the total number of users was 59. Based on the results of the web questionnaire survey, it was made evident that the system is useful for groups when meeting up, and the entry function for the nearest station to one’s home, as well as the recommendation function for meet-up stations, which was the original functions of the system, received generally good reviews. From the results of access analysis of the users’ log data, it was made evident that the system was used regardless of the type of device, just as the system was designed for, and that the system was used in harmony with the aim of the present study, which is to recommend meet-up stations for groups.

The volvocine algae represent an excellent model lineage in which to study evolution of female and male genders based on comparative analyses of related species. Among these species, Volvox carteri has been extensively studied as a model of an oogamous and complex organism. However, it may have unique derived features that are not present in other species of Volvox. Therefore, information regarding the characteristics of sexual reproduction of other species of Volvox is also important. In 1971, Starr studied four types of sexuality in several global strains identified as Volvox africanus; however, further taxonomic studies of these strains have been lacking, and strains of three of the four sexual types are not available. Here, we studied the morphology, sexual reproduction, and taxonomy of two V. africanus-like species isolated recently from Lake Biwa, Japan. These two species were very similar to two sexual types described by Starr in 1971: one producing dioecious sexual spheroids in heterothallic strains and the other forming both male spheroids and monoecious spheroids in a single strain. The former species produced zygotes with a reticulate cell wall, whereas a smooth zygote wall was observed in the latter species as in V. africanus previously reported from various localities around the world. Our multigene phylogenetic analysis demonstrated that these are sister species to each other. However, the presence of a compensatory base change in the most conserved region of the secondary structure of nuclear ribosomal DNA internal transcribed spacer-2, hybrid inviability demonstrated by intercrossing experiments, and morphological differences in the density of abutment between the gelatinous material of adjacent cells (individual sheaths) in the spheroid supported the recognition of the two species, V. africanus having a smooth zygote wall and V. reticuliferus Nozaki sp. nov. having a reticulate zygote wall.

The present study aimed to design, develop, operate and evaluate a sightseeing spot recommendation system for urban sightseeing spots in order to support individual, as well as group sightseeing activities while taking into consideration the user’s needs, which can change according to the circumstances (each user’s important conditions and sightseeing unit). The system was developed by integrating Web-GIS (Geographic Information Systems), the pairing system, the evaluation system, as well as the recommendation system into a single system, and it was also connected with external SNS (Social Networking Services: Twitter and Facebook). Additionally, the system was operated for four weeks in the central part of Yokohama City in Kanagawa Prefecture, Japan, and the total number of users was 52. Based on the results of the web questionnaire survey, the usefulness of the system when sightseeing was high, and the recommendation function of sightseeing spots, which is an original function, has received mainly good ratings. From the results of the access analysis of users’ log data, it is evident that the system has been used by different types of devices, just as it was designed for, and that the system has been used according to the purpose of the present study, which is to support the sightseeing activities of users.