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22. Angiotensin-converting enzyme regulates bradykinin receptor gene expression.

Author

Ignjacev-Lazich I, Kintsurashvili E, Johns C, Vitseva O, Duka A, Shenouda S, Gavras I, and Gavras H

Subjects
Angiotensin II Type 1 Receptor Blockers pharmacology, Animals, Blotting, Western, Cell Nucleus chemistry, Cells, Cultured, Cyclic AMP metabolism, Electrophoretic Mobility Shift Assay, Male, Muscle, Smooth, Vascular cytology, Muscle, Smooth, Vascular drug effects, NF-kappa B metabolism, RNA biosynthesis, RNA isolation & purification, Rats, Rats, Wistar, Receptor, Bradykinin B1 biosynthesis, Receptor, Bradykinin B1 genetics, Receptor, Bradykinin B2 biosynthesis, Receptor, Bradykinin B2 genetics, Transcription Factor AP-1 metabolism, Up-Regulation, Gene Expression Regulation physiology, Peptidyl-Dipeptidase A physiology, Receptors, Bradykinin biosynthesis, Receptors, Bradykinin genetics
Abstract

The angiotensin-converting enzyme (ACE) is a membrane-bound peptidyl dipeptidase known to act on a variety of peptide substrates in the extracellular space. Its most notable functions are the formation of angiotensin II and the degradation of bradykinin. In the current experiments, we found that exogenous ACE added to vascular smooth muscle cell culture strongly induces and upregulates the genes of bradykinin receptors B1 and B2. This transcriptional regulatory property of ACE was shown to be unrelated to its known enzymatic properties. Indeed, ACE at 3.75 microg/ml added in the culture medium of vascular smooth muscle cells was found to cause marked upregulation of the mRNA expression of the genes for the B1 and B2 receptors of bradykinin by 22- and 11-fold, respectively. This phenomenon was not altered by the addition of specific angiotensin II antagonists for the AT1 or AT2 receptors. Moreover, the ACE inhibitor captopril, which inhibited ACE enzymatic activity, did not block its effect at the bradykinin receptor gene transcription level. Expression of both receptor genes was completely abolished by actinomycin D. Furthermore, transcriptional upregulation was inhibited by curcumin, suggesting involvement of different transcriptional factors in this phenomenon. Electrophoretic mobility shift assay revealed increase in NF-kappaB and activator protein-1 protein binding for consensus sequences, between ACE-treated cells versus untreated cells. The data indicate a novel biological function of the ACE unrelated to its well-known enzymatic function as a peptidyl dipeptidase.

Published
2005
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