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SummaryHeterodimeric complexes incorporating the lipase-like proteins EDS1 with PAD4 or SAG101 are central hubs in plant innate immunity. EDS1 functions encompass signal relay from TIR domain-containing intracellular NLR-type immune receptors (TNLs) towards RPW8-type helper NLRs (RNLs) and, inA. thaliana, bolstering of signaling and resistance mediated by cell-surface pattern recognition receptors (PRRs). Biochemical activities underlying these mechanistic frameworks remain unknown.We used CRISPR/Cas-generated mutant lines and agroinfiltration-based complementation assays to interrogate functions of EDS1 complexes inN. benthamiana.We do not detect impaired PRR signaling inN. benthamianalines deficient in EDS1 complexes or RNLs. Intriguingly, mutations within the catalytic triad ofSolanaceaeEDS1 can abolish or enhance TNL immunity inN. benthamiana. Furthermore, nuclear EDS1 accumulation is sufficient forN. benthamianaTNL (Roq1) immunity.Reinforcing PRR signaling in Arabidopsis might be a derived function of the TNL/EDS1 immune sector. Dependency ofSolanaceaebut notA. thalianaEDS1 on catalytic triad residues raises the possibility that a TNL-derived small molecule binds to theSolanaceaeEDS1 lipase-like domain, and that EDS1 lipase-like domain pocket contributions to TNL immune responses vary between lineages. Whether and how nuclear EDS1 activity connects to membrane pore-forming RNLs remains unknown.

The evaluation of environmental aspects in the early planning phase of buildings can support the reduction of the resource use and environmental impacts associated with the building sector over the whole building life-cycle. The integration of life cycle assessment (LCA) benchmarks in the planning phase is one potential measure. To derive these benchmarks a large database of existing building assessment is essential. Potential data input is available from the German Sustainable Building Council (DGNB), as it certifies more than 200 buildings annually and the certification includes a mandatory LCA. In this study, the current submission files and database of the DGNB are assessed and critically reviewed with regard to their usability for automated LCA benchmarks. First, a harmonized database is created from the large number of assessed buildings. Second, the data is examined for its suitability for benchmarking with regard to data format, structure and level of detail. The data that were declared fit for purpose were used to create an exemplary, harmonized data set with 22 office buildings. The evaluation of these data for various environmental indicators of the individual life-cycle phases shows their respective relevance and can thus serve as a benchmark. Another focus is to encourage improvement of the additional documentation like the energy source required for better benchmarking, interpretation of results and auditing of the LCA rules for building certification. The results of this study highlight the opportunities and challenges in the development of a database for benchmarking. Before long-term LCA benchmarks can be developed and deployed, a standardized and uniform submission format of results, that is indifferent regarding the used LCA software, needs to be developed. In the future the submission process should be extended by an automated quality assurance to prevent restraints from low data quality and data gaps that otherwise have to be detected manually.

Existing buildings can reach a performance enhancement and extend their nominal service life through renovation measures such as seismic rehabilitation. In particular, when buildings have almost exhausted their service life, seeking an optimal solution should consider whether costs and environmental effects are worthwhile, or new construction is preferred. In this paper, a methodology to consider seismic hazard into probabilistic approaches for life-cycle analyses is presented considering the possibility of structural enhancement over an extended building lifespan. A life-cycle-based decision support tool for building renovation measures is developed and applied to a selected case study. Unlike standard “static” analyses, which in this work show shortcomings by underestimating impacts of vulnerable buildings, such an approach brings out environmental and economic advantages of retrofit measures designed to improve the structural performance.

Heterodimeric complexes containing the lipase-like protein ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) are regarded as central regulators of plant innate immunity. In this context, a complex of EDS1 with PHYTOALEXIN DEFICIENT4 (PAD4) is required for basal resistance and signaling downstream of immune receptors containing an N-terminal Toll-interleukin-1 receptor-like domain (TNLs) in Arabidopsis (

Summary Genome editing facilitated by Cas9-based RNA-guided nucleases (RGNs) is becoming an increasingly important and popular technique for reverse genetics in both model and non-model species. So far, RGNs were mainly applied for the induction of point mutations, and one major challenge consists in the detection of genome-edited individuals from a mutagenized population. Also, point mutations are not appropriate for functional dissection of non-coding DNA. Here, the multiplexing capacity of a newly developed genome editing toolkit was exploited for the induction of inheritable chromosomal deletions at six different loci in Nicotiana benthamiana and Arabidopsis. In both species, the preferential formation of small deletions was observed, suggesting reduced efficiency with increasing deletion size. Importantly, small deletions (

Building Information Modelling (BIM) supports construction processes by dealing with the variety and complexity of design in a single virtual model. The model may also be complemented by the static and energy performance of buildings. Facing the growing demand of sustainability strategies in the construction sector, the consideration of environmental information within the planning process influences the decision making of planners and stakeholders. Nevertheless, the life cycle assessment of buildings has been so far excluded in BIM, due to the high variety of accurate information and time required. In this paper, a systematic framework is presented and applied to a case study. BIM-LCA assists actors along the planning and designing phase, from the building conception as a whole, up to the elements’ details and materials’ definition. BIM and LCA intertwine in an application scheme of seven phases for integral planning and four levels of structural composition of a building. With respect to these, involved actors examine potential solutions through a tool which exploits alternative specifications in order to assess the environmental impacts. The goal of this paper is to demonstrate the application of a BIM-LCA model regarding decision making for reliable values of environmental impact in a given structural level of the building. The main findings of this framework are due to the multitude of actors and information orchestrated, namely to uncertainties which characterize the whole planning process and data handling. Through BIMLCA, actors are assisted by ensuring flexibility of models and consistency of results throughout planning and designing.

Technological advancement culminating in a globalized economy has brought tremendous improvements for mankind in manifold respects but comes at the cost of alienation from nature. Human activities nowadays are unsustainable and cause severe damage especially in terms of global depletion and destabilization of natural systems but also harm its own social resources. In this paper, a sustainability assessment method is developed based on a bio-inspired sustainability framework that has been developed in the project TRR 141-C01 “The biomimetic promise.” It is aims at regaining the advantages of societal embeddedness in its environment through biological inspiration. The method is developed using a structured approach including requirement specification, description of the inventory models on bio-inspiration and sustainability assessment, creation of a bio-inspired sustainability assessment model and its validation. It is defined as an accompanying assessment for decision support, using a six-fold two-dimensional structure of social, economic and environmental functions and burdens. The method is applied and validated in 6 projects of TRR 141 and its applicability is exemplarily shown by the assessment of “Bio-flexi”, a biobased and biodegradable natural fiber reinforced plastic composite for indoor cladding applications. Based on the findings of the application the assessment method itself is proposed to be advanced towards an adaptive structure and a consequent outlook is provided.

Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. This combinatorial assembly strategy meets the increasingly complex demands in biotechnology and bioengineering, and also represents a cost-efficient and versatile alternative to previous molecular cloning techniques. For Modular Cloning, a collection of commonly used Plant Parts was previously released together with the Modular Cloning toolkit itself, which largely facilitated the adoption of this cloning system in the research community. Here, a collection of approximately 80 additional phytobricks is provided. These phytobricks comprise e.g. modules for inducible expression systems, different promoters or epitope tags, which will increase the versatility of Modular Cloning-based DNA assemblies. Furthermore, first instances of a “peripheral infrastructure” around Modular Cloning are presented: While available toolkits are designed for the assembly of plant transformation constructs, vectors were created to also use coding sequence-containing phytobricks directly in yeast two hybrid interaction or bacterial infection assays. Additionally, DNA modules and assembly strategies for connecting Modular Cloning with Gateway Cloning are presented, which may serve as an interface between available resources and newly adopted hierarchical assembly strategies. The presented material will be provided as a toolkit to the plant research community and will further enhance the usefulness and versatility of Modular Cloning.

Technological advancement culminating in a globalized economy has brought tremendous improvements for mankind in manifold respects but comes at the cost of alienation from nature. Human activities nowadays are unsustainable and cause severe damage especially in terms of global depletion and destabilization of natural systems but also harm its own social resources. In this paper, a sustainability assessment method is developed based on a bio-inspired sustainability framework that has been developed in the project TRR 141-C01 “The biomimetic promise.” It is aims at regaining the advantages of societal embeddedness in its environment through biological inspiration. The method is developed using a structured approach including requirement specification, description of the inventory models on bio-inspiration and sustainability assessment, creation of a bio-inspired sustainability assessment model and its validation. It is defined as an accompanying assessment for decision support, using a six-fold two-dimensional structure of social, economic and environmental functions and burdens. The method is applied and validated in 6 projects of TRR 141 and its applicability is exemplarily shown by the assessment of “Bio-flexi”, a biobased and biodegradable natural fiber reinforced plastic composite for indoor cladding applications. Based on the findings of the application the assessment method itself is proposed to be advanced towards an adaptive structure and a consequent outlook is provided.

The EU building stock is relatively old with 40% of it built before 1960. In Albania the building sector accounts for 26, 9% of final energy consumption, offering high energy saving potentials due to the great number of old residential buildings. Intervention is not always possible and, in order to achieve significant environmental savings, the national action plans cannot rely only on the physical improvement of existing buildings. This paper proposes a probabilistic LCA and LCC evaluation model using MC simulation, for the prediction of intervention options in existing buildings. The potential environmental and economic impacts of three intervention options: standard, ambitious retrofitting and new construction during the whole life cycle of a building are analysed. A framework is defined, with the purpose of estimating the value of a building in a specific time during its life cycle. Comparing the generated values of potential environmental impacts and associating them with the changes on the buildings value enables the process of deciding upon the most desirable and/or agreed combination. The results of the SLED Study 2015 on Albanian building typology are used, while the new construction model is defined according to German EnEV2014 requirements. The GWP values from the LCA/LCC assessment of the intervention scenarios, done through the SBS tool of Fraunhofer IBP, are used to create a prediction model for future alternative solutions especially in early planning phase. Decision-making through this model can encourage a sustainability strategy for energy efficiency improvement in the building sector of Albania.

Over the last few decades, the systematic approach of knowledge transfer from biological concept generators to technical applications has received increasing attention, particularly because marketable bio-derived developments are often described as sustainable. The objective of this paper is to rationalize and refine the discussion about bio-derived developments also with respect to sustainability by taking descriptive, normative and emotional aspects into consideration. In the framework of supervised learning, a dataset of 70 biology-derived and technology-derived developments characterised by 9 different attributes together with their respective values and assigned to one of 17 classes was created. On the basis of the dataset a decision tree was generated which can be used as a straightforward classification tool to identify biology-derived and technology-derived developments. The validation of the applied learning procedure achieved an average accuracy of 90.0%. Additional extraordinary qualities of technical applications are generally discussed by means of selected biology-derived and technology-derived examples with reference to normative (contribution to sustainability) and emotional aspects (aesthetics and symbolic character). In the context of a case study from the building sector, all aspects are critically discussed.