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Shengli Oilfield has abundant low-permeability oil reservoirs with proven reserves of 1.18 billion tons， presenting a good prospect for development. However， there is a problem of inability to inject and produce during the development process of the oil reservoirs due to the low porosity， poor permeability， and strong heterogeneity of the low-permeability oil reservoir. A systematic review was conducted on the prediction and analysis methods for low-permeability oil reservoirs in Shengli Oilfield to achieve efficient development of low-permeability oil reservoirs， as well as efficient development technologies， and the advantages and disadvantages of existing technologies were analyzed to provide an outlook for the development technologies of low-permeability oil reservoirs. Based on research and practice， the following conclusions are drawn： ① Shengli Oilfield has developed fine reservoir characterization technologies such as reservoir prediction， in-situ stress prediction， and evaluation of reservoir heterogeneity. ② Based on theoretical and technological innovation， the physical limit of reserve production was constantly broken， forming differential development technology series， such as water injection development technology of horizontal wells in ultra-low permeability oil reservoirs， long well section multi-stage fracturing development technology of horizontal wells in tight oil reservoirs， well pattern adaptation technology for enhanced oil recovery of general low-permeability oil reservoirs， pressure flooding technology in low-permeability tight oil reservoirs， and viscous emulsion-surfactant flooding technology. Good development results have been achieved. ③ Combined with the actual needs of high-quality development and speed and efficiency improvement development of low-permeability oil reservoirs in Shengli Oilfield， the well pattern reconstruction technology of dual control streamline variation， CCUS technology， and nanobubble flooding technology were proposed， pointing out the development direction for improving oil recovery of low-permeability oil reservoirs.

In this paper, the 3-D model of discontinuous contact of 2/3 lay length steel rope is established by ProE/5.0. Finite element analysis of discontinuous contact steel rope under axial tension and torsion load was carried out in ABAQUS. The wear test of wire rope was carried out on the discontinuous contact steel wire rope damage test bench, and the distribution of wear marks on the worn steel wire surface was analyzed by optical microscope（OM）. The results show that the equivalent stress, axial stress and axial deformation are spiral distribution along the axial direction of the wire rope; on the strand section, the equivalent stress increases gradually from the core wire to the side wire, the maximum value appears in the contact area of rope groove, the experimental results show that the steel wire surface also presents elliptical wear scar, which is consistent with the simulation results.

Self-organized blastoids from extended pluripotent stem (EPS) cells possess enormous potential for investigating postimplantation embryo development and related diseases. However, the limited ability of postimplantation development of EPS-blastoids hinders its further application. In this study, single-cell transcriptomic analysis indicated that the "trophectoderm (TE)-like structure" of EPS-blastoids was primarily composed of primitive endoderm (PrE)-related cells instead of TE-related cells. We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure. Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation. Furthermore, we demonstrated that blastocyst-like structures reconstituted by combining the EPS-derived bilineage embryo-like structure (BLES) with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses. In summary, our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro. [ABSTRACT FROM AUTHOR]

Abstract: A sequencing batch reactor (SBR) was used to remove COD, NH4 +-N and PO4 3--P in slaughterhouse wastewater at different temperatures. A complete operation cycle was 7h, which consisted of fill, aeration(5h), settling(1h), stationary(1h) and drawing. Conventional treatment technologies were unsuccessful in treating slaughterhouse wastewater because it contained substantial amounts of fat, oil and grease (FOG). The SBR process showed great nutrient removal performances after 7 days prefermentation. The removals of COD, NH4 +-N and PO4 3--P were 99%, 85% and 99%, respectively. The increase in effluent volatile fatty acid (VFA) and phosphate concentrations after prefermentation may explain the high levels of biological carbon, nitrogen and phosphorus removal observed. The results also showed that 30 C was found to be the most suitable temperature as the removal efficiency of COD, NH4 +-N and PO4 3--P was 96%, 92% and 91%, respectively. [Copyright &y& Elsevier]

Objective: To investigate the effect of yoga on anxiety and fetal weight of pregnant women carrying fetus small for gestational age (SGA)., Methods: In this retrospective analysis, a total of 186 pregnant women with SGA fetus in our hospital from January 2015 to December 2017 were enrolled in this study. Among them, 90 patients received routine check-up were included in the control group, and the other 96 patients who had professional yoga exercise were included in the observation group. The differences of anxiety scale scores and fetal weight between the two groups before and after intervention were compared., Results: There was no significant difference in scores of anxiety scale (SAS) between the two groups before intervention. After intervention, the SAS score of pregnant women in intervention group was (46.48±3.79) was significantly lower than that in control group (60.13±4.25). There was also significant difference in fetal growth trajectory between the two groups, with a significant increase of 1021.36 g in the intervention group compared with 795.62 g in the control group (P<0.05). Furthermore, single regression analysis showed that average gestational weeks (r=0.064. P=0.011), yoga exercise (r=0.043, P<0.001), forceps use (r=0.338, P<0.001) and conversion to cesarean section (r=0.431, P<0.001) showed a significant correlation with anxiety and fetus growth in pregnant women carrying SGA fetus. Multiple regression analysis showed that yoga exercise (P<0.001) was selected as independent variables in the multiple regression model of anxiety and fetus growth in pregnant women with SGA fetus., Conclusion: Yoga can effectively reduce the anxiety of pregnant women with small gestational age fetus and good for the growth and development of the fetus., Competing Interests: None., (AJTR Copyright © 2022.)

Natural killer (NK) cell cytotoxicity is an effective defense against metastatic tumor cells or viruses in the blood. However, NK cytotoxicity against tumor cells may be inhibited by a fibrinogen coat adhered to the surface of tumor cells. Ganoderma lucidum (G. lucidum) polysaccharides have been reported for their inhibitory ability on the adhesion of type I collagen, hyaluronan, fibronectin and laminin to integrins that were highly expressed on melanoma cells, and were therefore capable of enhancing NK cytotoxicity to tumor cells. In this study, we investigated the effect of G. lucidum polysaccharides on fibrinogen's adhesion to melanoma cells and NK cytotoxicity to tumor cells. Melanoma cells B16 and A375 were cultured and analyzed using flow cytometry. Human NK cells were isolated and analyzed using an NK cytotoxic assay. The results showed that polysaccharides extracted from G. lucidum inhibit the adhesion of fibrinogen to melanoma cells, and reverse the blocking effect of the fibrin coat on NK cytotoxicity against melanoma cells.