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Pasteurella multocida is a multi-host pathogen that infects a wide spectrum of domestic and wild animals including humans. Despite its impact on health and economics, P. multocida is considered an enigmatic pathogen and the genetic basis of its pathogenicity and host adaptation still remains unclear. Here we present a detailed genomic framework based on 336 whole-genome sequences of P. multocida isolates from different animal species and countries. Our data provide genomic support of the existence of two very divergent phylogroups (PmI and PmII), which present a barrier to homologous recombination suggesting genetic isolation. Additionally, a torCAD operon, which reduces TMAO (trimethylamine N-oxide) to produce energy during bacterial anaerobic respiration, is present only in PmI and can act as a hypothetical driver of niche segregation between phylogroups. The PmI phylogroup harbors strains that infect a wider range of hosts than PmII, and shows a highly diverse phylogeny and accessory genome. We identified nine clonal lineages for PmI, seven of which are associated with specific hosts or diseases and contain distinct accessory gene pools that can confer ecologically relevant phenotypes. We found differential presence of a trehalose metabolism operon in the bovine lineage associated with pneumonic pasteurellosis; while, citrate, L-arabinose, L-fucose, and D-allose operons are only present in avian lineages. These findings suggest that alternative metabolic pathways may facilitate the establishment of P. multocida during host colonization in the early stages of infection promoting the adaptation of P. multocida lineages to certain hosts.

Background Despite their regional economic importance and being increasingly reared globally, the origins and evolution of the llama and alpaca remain poorly understood. Here we report reference genomes for the llama, and for the guanaco and vicuña (their putative wild progenitors), compare these with the published alpaca genome, and resequence seven individuals of all four species to better understand domestication and introgression between the llama and alpaca. Results Phylogenomic analysis confirms that the llama was domesticated from the guanaco and the alpaca from the vicuña. Introgression was much higher in the alpaca genome (36%) than the llama (5%) and could be dated close to the time of the Spanish conquest, approximately 500 years ago. Introgression patterns are at their most variable on the X-chromosome of the alpaca, featuring 53 genes known to have deleterious X-linked phenotypes in humans. Strong genome-wide introgression signatures include olfactory receptor complexes into both species, hypertension resistance into alpaca, and fleece/fiber traits into llama. Genomic signatures of domestication in the llama include male reproductive traits, while in alpaca feature fleece characteristics, olfaction-related and hypoxia adaptation traits. Expression analysis of the introgressed region that is syntenic to human HSA4q21, a gene cluster previously associated with hypertension in humans under hypoxic conditions, shows a previously undocumented role for PRDM8 downregulation as a potential transcriptional regulation mechanism, analogous to that previously reported at high altitude for hypoxia-inducible factor 1α. Conclusions The unprecedented introgression signatures within both domestic camelid genomes may reflect post-conquest changes in agriculture and the breakdown of traditional management practices.

This paper examines the impact on woodlands associated with burnt mound use from floodplain sediments and peats, using a combination of pollen, non-pollen palynomorphs, micro- and macro-charcoal and worked wood for the first time. We present this data from a multi-period burnt mound complex, dating from the Late Neolithic to the Medieval period, at Ballygawley, Co. Tyrone, Northern Ireland, to reconstruct vegetation changes from the Neolithic onwards and establish the significance of these changes, in particular on woodlands whilst the burnt mounds were in use. The findings from the macroscopic charcoal suggests the most abundant trees were commonly, but not exclusively, exploited. Local woodland was seemingly unaffected by use of burnt mounds during the Neolithic and early Bronze Age based on pollen evidence. A sustained increase in microscopic charcoal coincides with a permanent decrease in alder-carr woodland during a period of near continuous burnt mound use between 1725 and 530 BC, and a seco...

Burnt mounds, or fulachtaí fiadh as they are known in Ireland, are probably the most common prehistoric site type in Ireland and Britain. Typically Middle–Late Bronze Age in age (although both earlier and later examples are known), they are artefact-poor and rarely associated with settlements. The function of these sites has been much debated with the most commonly cited uses being for cooking, as steam baths or saunas, for brewing, tanning, or textile processing. A number of major infrastructural development schemes in Ireland in the years 2002–2007 revealed remarkable numbers of these mounds often associated with wood-lined troughs, many of which were extremely well-preserved. This afforded an opportunity to investigate them as landscape features using environmental techniques – specifically plant macrofossils and charcoal, pollen, beetles, and multi-element analyses. This paper summarises the results from eight sites from Ireland and compares them with burnt mound sites in Great Britain. The fulachtaí fiadh which are generally in clusters, are all groundwater-fed by springs, along floodplains and at the bases of slopes. The sites are associated with the clearance of wet woodland for fuel; most had evidence of nearby agriculture and all revealed low levels of grazing. Multi-element analysis at two sites revealed elevated heavy metal concentrations suggesting that off-site soil, ash or urine had been used in the trough. Overall the evidence suggests that the most likely function for these sites is textile production involving both cleaning and/or dyeing of wool and/or natural plant fibres and as a functionally related activity to hide cleaning and tanning. Whilst further research is clearly needed to confirm if fulachtaí fiadh are part of the ‘textile revolution’ we should also recognise their important role in the rapid deforestation of the wetter parts of primary woodland and the expansion of agriculture into marginal areas during the Irish and British Bronze Ages.

This paper provides a summary of the palaeoenvironmental evidence from a spread of late Mesolithic burnt material and two late Neolithic to early Bronze Age burnt mounds. The burnt mounds were up to 10 m diameter, had an amorphous shape, and were consistently less than 0.8 m thick. Monoliths were collected from two sites, Ballygawley and Roughan, in Co. Tyrone, Northern Ireland. This provided an opportunity to use a detailed palaeoecological approach for the first time to investigate the use and function of burnt mounds. Pollen, non-pollen palynomorphs, micro- and macroscopic charcoal were used to place these features within their environmental context, and to establish if such an approach could provide further insights into their function. The palynological results shared similar characteristics: high microscopic charcoal values, repetitive fluctuations in tree and shrub taxa, increased Sphagnum, and the presence of non-pollen palynomorphs (NPPs) HdV-114 and HdV-146, all of which could be diagnostic indicators of burnt mounds in palynological records. While the data do not allow us to ascribe a specific function for the burnt mounds, their environmental setting is discussed. A “see-saw” pattern of arboreal pollen, combined with the macroscopic charcoal data, indicate possible species selection and management of local woodland for fuelwood.

Summary Many peatland ecosystems in Europe have become degraded in the last century owing to the effects of drainage, burning, pollution and climate change. There is a need to understand the drivers of peatland degradation because management and restoration interventions are expensive and can affect the natural ecohydrological dynamics of such sensitive environments. However, if given enough time, peatlands may have the ability to recover spontaneously without deliberate action. We use a detailed multiproxy palaeoecological data set from a degraded raised bog in Northern England to examine its ecosystem stability and long-term dynamics in response to anthropogenic disturbance over a variety of timescales. One feature of many degraded peatlands (including our study site) is the local dominance of Molinia caerulea (purple moor-grass), which has expanded at the expense of characteristic peatland plants, including sedges and Sphagnum mosses. Our data show that there has been a long history of human impacts at the site which have culminated in its current unfavourable condition. Several distinct episodes of past peat cutting are evident as hiatuses in peat accumulation; however, peat accumulation and plant community structure have subsequently recovered spontaneously. The appearance of M. caerulea occurs coevally with an unprecedented variety of recent anthropogenic impacts, all of which have contributed to providing a suitable environment for its rise to dominance. We have dated the appearance of M. caerulea to the latter half of the twentieth century which corresponds to a number of anthropogenic press disturbances, including the following: dust loading from post-war expansion of the adjacent quarry; burning; drainage; airborne pollution; and contamination from soil dust and agrochemicals. [Synthesis] Our study demonstrates the importance of palaeoecology for understanding the trajectories of peatland development and ecosystem dynamics, including their resilience and resistance to pulse and press disturbances. We show that peatlands have the capability to recover spontaneously from severe disturbances such as peat cutting, albeit on timescales much longer than those applied to contemporary monitoring of restoration efforts. The implications are relevant for determining whether it is preferable to manage and restore peatlands, or to allow them to recover naturally without human intervention.

Purpose – This paper aims to investigate the role of Sun Tzu’s significant strategies on the relationship between the institutional environment and international performance of Chinese born global firms, a type of small- and medium-sized enterprise (SME) characterized by the company’s limited resources and its early efforts to internationalize. Design/methodology/approach – The methodology is based on a multi-case analysis of interviews conducted with four chosen born global firms, coupled with public database and Web site searches. Through the use of qualitative methods, propositions were developed. Findings – This paper provides insights regarding how the institutional environment, both formal and informal, has a strong positive relationship with born global firm’s international performance. Moreover, Sun Tzu’s significant strategies play a critical role in the internationalization process of born global firms in emerging markets. Originality/value – Although existing studies discuss the application of Eastern philosophical strategies adopted by firms in emerging markets, to the best of our knowledge, this is one of the earliest studies which evaluates the moderation effect of Sun Tzu’s significant strategies on the relationship between institutional environment and business performance. The paper contributes to scholarly discourse on the influencing factors of born global firm’s internationalization process. It also has practical relevance to international entrepreneurs and SMEs from emerging markets.

Airports are a crucial part of the United States transportation system and airport officials are continuously challenged to ensure that their operations are secure. A major part of providing airport security is implementing check points where passengers interface with the security screening system. The airlines and airport security administration receive negative publicity when passengers are mistreated at security check points. Further, these organizations spend a lot of time to assess the situation and take corrective actions. This study examines passenger perceived bias and the airport security screening system. Using data from a randomly selected sample of 145 airline passengers, six hypotheses are tested. The hypotheses exam the predictive relationships of control procedures, security personnel training, security personnel professionalism, and security screening systems on passenger perceived bias. Multiple regression analysis is used to test the hypotheses. The research findings indicate that security personnel professionalism is negatively and significantly related to passenger perceived bias. Also, security personnel training and security control procedures are positively and significantly related to security personnel professionalism. Management implications, limitations, and recommendations for future research are offered.

Vicuñas and guanacos are two species of wild South American camelids that are key ruminants in the ecosystems where they occur. Although closely related, these species feature differing ecologies and life history characters, which are expected to influence both their genetic diversity and population differentiation at different spatial scales. Here, using mitochondrial and microsatellite genetic markers, we show that vicuña display lower genetic diversity within populations than guanaco but exhibit more structure across their Peruvian range, which may reflect a combination of natural genetic differentiation linked to geographic isolation and recent anthropogenic population declines. Coalescent-based demographic analyses indicate that both species have passed through a strong bottleneck, reducing their effective population sizes from over 20,000 to less than 1000 individuals. For vicuña, this bottleneck is inferred to have taken place ~3300 years ago, but to have occurred more recently for guanaco at ~2000 years ago. These inferred dates are considerably later than the onset of domestication (when the alpaca was domesticated from the vicuña while the llama was domesticated from the guanaco), coinciding instead with a major human population expansion following the mid-Holocene cold period. As importantly, they imply earlier declines than the well-documented Spanish conquest, where major mass mortality events were recorded for Andean human and camelid populations. We argue that underlying species’ differences and recent demographic perturbations have influenced genetic diversity in modern vicuña and guanaco populations, and these processes should be carefully evaluated in the development and implementation of management strategies for these important genetic resources.

The causes of Late Pleistocene megafaunal extinctions (60, 000 to 11, 650 years ago, hereafter 60 to 11.65 ka) remain contentious, with major phases coinciding with both human arrival and climate change around the world. The Americas provide a unique opportunity to disentangle these factors as human colonization took place over a narrow timeframe (∼15 to 14.6 ka) but during contrasting temperature trends across each continent. Unfortunately, limited data sets in South America have so far precluded detailed comparison. We analyze genetic and radiocarbon data from 89 and 71 Patagonian megafaunal bones, respectively, more than doubling the high-quality Pleistocene megafaunal radiocarbon data sets from the region.Weidentify anarrowmegafaunal extinction phase 12, 280 ± 110 years ago, some 1 to 3 thousand years after initial human presence in the area. Although humans arrived immediately prior to a cold phase, the Antarctic Cold Reversal stadial, megafaunal extinctions did not occur until the stadial finished and the subsequent warming phase commenced some 1 to 3 thousand years later. The increased resolution provided by the Patagonianmaterial reveals that the sequence of climate andextinctionevents inNorthandSouth America were temporally inverted, but in both cases, megafaunal extinctions did not occur until human presence and climate warming coincided. Overall, metapopulation processes involving subpopulation connectivity on a continental scale appear to have been critical for megafaunal species survival of both climate change and human impacts., La lista completa de autores que integran el documento puede consultarse en el archivo, Facultad de Ciencias Naturales y Museo

PurposeThe purpose of this paper is to explore the relationship between buyer's emotional intelligence and buyer's relationship performance.Design/methodology/approachA survey methodology was used to collect data for this study. The survey was administered using an assessment process which incorporated “buyers'” and “suppliers'” assessment for the independent variable, emotional intelligence, and the dependent variable, relationship performance. The respondents consisted of 34 buyers and 102 suppliers.FindingsCorrelation and regression analysis revealed several significant relationships between the variables. Specifically, buyers' self‐assessed emotional intelligence was not significantly related to buyers' self‐assessed relationship performance. Buyers' emotional intelligence assessed by suppliers was significantly related to buyers' relationship performance assessed by suppliers. Buyers' emotional intelligence (as assessed by buyers and suppliers' assessment differences) was not significantly related to buyers' self‐assessed relationship performance. Last, buyers' emotional intelligence (as assessed by buyers' and suppliers' assessment differences) was significantly related to buyers' relationship performance (as assessed by buyers' and suppliers' assessment differences). Thus, the results suggest that buyers' emotional intelligence is positively related to relationship performance, most significantly from the perspective of their key suppliers.Originality/valueThis study is the first to introduce emotional intelligence within an environment that consisted of individuals working with others outside of their respective organizations (buyers and suppliers). This research offers some insight to buyers on the implications of emotional intelligence and how it can be used to support their interactions with their key suppliers.

PurposeThe purpose of this research is to examine the interactions between individuals and the social environment as individuals engage in self‐directed learning, a predecessor to intentional change theory. The individuals are graduates of a part‐time MBA program and the social environment is the life sphere framework introduced with this study. Self‐directed learning refers to the learning agendas that these people outlined for themselves when they first entered the MBA program.Design/methodology/approachLongitudinal assessment data were collected using the critical incident interview, the learning skills profile, and the life sphere interview. Correlations were run testing the hypotheses: as the number of life spheres, relationships, or activities increases, so will the demonstration of the targeted competencies; and if the participant is able to work on developing these competencies in the life sphere that he or she designates as primary to the learning goal, then the demonstration of these competencies will improve.FindingsThe number of life spheres, relationships, and activities does impact positively on the demonstration of these competencies and those participants for whom the work organization life sphere is primary to learning goal achievement showed improved demonstration of the targeted competencies.Research limitations/implicationsThe study needs to be replicated with a larger sample size. This initial study was merely exploratory in nature.Originality/valueAs society approaches the virtual office it makes sense that it will get what it needs, when it needs it – no matter where one is or to whom one is talking. With these changes has come the need to find resources in places and from people that may not have been considered previously. The relationships and activities in one's social environment are more critical than ever before.

There has been recent debate over stratigraphic markers used to demarcate the Anthropocene from the Holocene Epoch. However, many of the proposed markers are found only in limited areas of the world or do not reflect human impacts on the environment. Here we show that spheroidal carbonaceous particles (SCPs), a distinct form of black carbon produced from burning fossil fuels in energy production and heavy industry, provide unambiguous stratigraphic markers of the human activities that have rapidly changed planet Earth over the last century. SCPs are found in terrestrial and marine sediments or ice cores in every continent, including remote areas such as the high Arctic and Antarctica. The rapid increase in SCPs mostly occurs in the mid-twentieth century and is contemporaneous with the ‘Great Acceleration’. It therefore reflects the intensification of fossil fuel usage and can be traced across the globe. We integrate global records of SCPs and propose that the global rapid increase in SCPs in sedimentary records can be used to inform a Global Standard Stratigraphic Age for the Anthropocene. A high-resolution SCP sequence from a lake or peatland may provide the much-needed ‘Golden Spike’ (Global Boundary Stratotype Section and Point).

We used in-depth critical incident interviews with the external leaders of self-managing work teams and their team members, and interviews and surveys provided by managers, to understand how effective leader behaviors and strategies unfold over time. Content analyses of the data produced a process model showing that effective external leaders move back and forth across boundaries to build relationships, scout necessary information, persuade their teams and outside constituents to support one another, and empower their teams to achieve success.

Mitochondrial DNA sequences isolated from ancient dog remains from Latin America and Alaska showed that native American dogs originated from multiple Old World lineages of dogs that accompanied late Pleistocene humans across the Bering Strait. One clade of dog sequences was unique to the New World, which is consistent with a period of geographic isolation. This unique clade was absent from a large sample of modern dogs, which implies that European colonists systematically discouraged the breeding of native American dogs.

The origins of South America's domestic alpaca and llama remain controversial due to hybridization, near extirpation during the Spanish conquest and difficulties in archaeological interpretation. Traditionally, the ancestry of both forms is attributed to the guanaco, while the vicuña is assumed never to have been domesticated. Recent research has, however, linked the alpaca to the vicuña, dating domestication to 6000-7000 years before present in the Peruvian Andes. Here, we examine in detail the genetic relationships between the South American camelids in order to determine the origins of the domestic forms, using mitochondrial (mt) and microsatellite DNA. MtDNA analysis places 80% of llama and alpaca sequences in the guanaco lineage, with those possessing vicuña mtDNA being nearly all alpaca or alpaca-vicuña hybrids. We also examined four microsatellites in wild known-provenance vicuña and guanaco, including two loci with non-overlapping allele size ranges in the wild species. In contrast to the mtDNA, these markers show high genetic similarity between alpaca and vicuña, and between llama and guanaco, although bidirectional hybridization is also revealed. Finally, combined marker analysis on a subset of samples confirms the microsatellite interpretation and suggests that the alpaca is descended from the vicuña, and should be reclassified as Vicugna pacos. This result has major implications for the future management of wild and domestic camelids in South America.

We examine mid- to late Holocene centennial-scale climate variability in Ireland using proxy data from peatlands, lakes and a speleothem. A high degree of between-record variability is apparent in the proxy data and significant chronological uncertainties are present. However, tephra layers provide a robust tool for correlation and improve the chronological precision of the records. Although we can find no statistically significant coherence in the dataset as a whole, a selection of high-quality peatland water table reconstructions co-vary more than would be expected by chance alone. A locally weighted regression model with bootstrapping can be used to construct a ‘best-estimate’ palaeoclimatic reconstruction from these datasets. Visual comparison and cross-wavelet analysis of peatland water table compilations from Ireland and Northern Britain shows that there are some periods of coherence between these records. Some terrestrial palaeoclimatic changes in Ireland appear to coincide with changes in the North Atlantic thermohaline circulation and solar activity. However, these relationships are inconsistent and may be obscured by chronological uncertainties. We conclude by suggesting an agenda for future Holocene climate research in Ireland.

Actualmente es posible determinar el estatus de especies silvestres mediante el analisis de ADN extraido de muestras no invasivas como las heces. Aunque dichos analisis suelen ser dificultosos debido principalmente a la composicion y conservacion de las heces, se ha demostrado en muchas especies que con el desarrollo de metodos adecuados de extraccion es posible maximizar la fiabilidad y eficacia de este procedimiento. Por tanto, el objetivo del presente estudio fue optimizar un metodo de extraccion de ADN de heces de vicuna Vicugna vicugna mensalis, especie clasificada como casi amenazada en el Peru, con la finalidad de obtener ADN de suficiente calidad para analisis moleculares. Se recolectaron 51 muestras de heces durante los meses de agosto y septiembre de 2008 en dos comunidades vicuneras ubicadas a mas de 4600 msnm en los departamentos de Junin y Moquegua. Luego de observar la defecacion, las muestras fueron recolectadas en forma inmediata, conservadas en etanol y transportadas al laboratorio para la extraccion del ADN. El kit comercial QIAamp® DNA Stool Mini Kit (QIAGEN) fue modificado para incluir un vigoroso y extenso proceso de agitacion y los resultados fueron comparados con dos protocolos comunmente utilizados: fenol/cloroformo/alcohol isoamilico y el mismo kit QIAGEN. La cantidad del ADN extraido fue observado en electroforesis horizontal en geles de agarosa, y la calidad evaluada mediante la amplificacion y visualizacion de los tamanos esperados de los microsatelites YWLL46 y LCA19. Aunque se logro extraer ADN con los tres metodos, solamente el metodo modificado permitio obtener ADN de suficiente calidad para ser utilizado en pruebas moleculares. Palabras clave: extraccion de ADN, heces, vicuna

Se reporta el desarrollo y optimizaciones de tecnicas moleculares (PCR simple, mul- tiple y semi-anidada) para determinar el sexo de camelidos sudamericanos (CSA) amplifi- cando la secuencia del gen Zinc Finger Protein (ZF). La tecnica utilizo ADN obtenido de 28 muestras de sangre de alpacas, llamas y vicunas, 20 muestras de heces de vicunas y guanacos conservadas en etanol al 96%, y 22 embriones de alpaca colectados entre 72 y 96 horas postmonta y preservados en etanol. Las muestras de ADN de sangre y heces fueron extraidas usando kits comerciales, y las de embriones aplicando tres metodos (ebullicion, proteinasa K y fenol-cloroformo). Una vez optimizada la PCR simple para la deteccion de los genes ZFY y ZFX, se implemento la PCR multiple para ADN de sangre y heces y la PCR semi-anidada para ADN de embriones. La tecnica de PCR multiple deter- mino el sexo correctamente en el 100% de las muestras de ADN sanguineo, en el 87.5% de muestras de ADN de heces colectadas en 2008 y en el 50% de las muestras de ADN de heces colectadas en 2004 y preservadas durante cuatro anos antes del analisis. La prue- ba de PCR semi-anidada, sin embargo, no pudo ser optimizada

Guanacos (Lama guanicoe) are the most important native herbivorous species in the South American steppes and the dominant ungulate in a fauna rich in rodents but poor in large mammal species. Between 2 and 4 subspecies are usually recognized within Lama guanicoe, based on subtle morphological differences and geographic distribution. To evaluate whether molecular variation is consistent with the latter hypotheses, we analyzed the complete cytochrome-b and partial control region mitochondrial DNA sequences of L. guanicoe from 22 localities in Peru, Bolivia, Argentina, and Chile. Sequence analyses of both genes support the monophyly of the species but failed to distinguish the occurrence of subspecies along the geographic range. Despite that, the northernmost populations (Peru and northern Chile) showed some degree of genetic differentiation with respect to southern representatives from Argentina, Bolivia, and rest of Chile. Analysis of genetic diversity also showed a strong signal of past low population size and a recent population expansion.

Four camelid species exist in South America: two wild, the guanaco (Lama guanicoe) and the vicuna (Vicugna vicugna), and two domestic, the alpaca (Lama pacos) and the llama (Lama glama). However, the origin of the domestic species has been a matter of debate. In the present study, variations in chromosome G banding patterns and in two mitochondrial gene sequences have been used to study the origin and classification of the llama and alpaca.-Similar patterns in chromosome G band structure were observed in all four Lamini species, and these in turn were similar to the bands described for camels, Camelus bactrianus. However, fine and consistent differences were found in the short arms of chromosome 1, separating camels, guanacos and llamas from vicunas and alpacas. This pattern was consistent even in a hybrid guanaco x alpaca. Equivalent relationship showed the complete cytochrome b gene sequences, and the minimum expansion tree of the partial control region sequence, grouping guanaco with llama and vicuna with alpaca. Phylogenetic analyses showed V. vicugna and L. guanicoe as monophyletic groups. Analysis of both gene sequences revealed two clades within vicuna, concordant with the two described subspecies, but the results for guanaco did not confirm existence of the four previously proposed subspecies. The combined analysis of chromosomal and molecular variation showed close genetic similarity between alpacas and vicunas, as well as between llamas and guanacos. Although directional hybridization was revealed, our results strongly support the hypothesis that the llama would have derived from L. guanicoe and the alpaca from V. vicugna, supporting reciassification as V. pacos.

The vicuña (Vicugna vicugna; Miller, 1924) is a conservation success story, having recovered from near extinction in the 1960s to current population levels estimated at 275,000. However, lack of information about its demographic history and genetic diversity has limited both our understanding of its recovery and the development of science-based conservation measures. To examine the evolution and recent demographic history of the vicuña across its current range and to assess its genetic variation and population structure, we sequenced mitochondrial DNA from the control region (CR) for 261 individuals from 29 populations across Peru, Chile and Argentina. Our results suggest that populations currently designated as Vicugna vicugna vicugna and Vicugna vicugna mensalis comprise separate mitochondrial lineages. The current population distribution appears to be the result of a recent demographic expansion associated with the last major glacial event of the Pleistocene in the northern (18 to 22 degrees S) dry Andes 14-12,000 years ago and the establishment of an extremely arid belt known as the 'Dry Diagonal' to 29 degrees S. Within the Dry Diagonal, small populations of V. v. vicugna appear to have survived showing the genetic signature of demographic isolation, whereas to the north V. v. mensalis populations underwent a rapid demographic expansion before recent anthropogenic impacts.

Diez microsatélites polimórficos para alpacas y llamas fueron usados para evaluar el parentesco en 47 alpacas (18 crías, 18 madres y 11 padres) de la Estación Experimental IVITA-Maranganí, provincia de Canchis (Cusco, Perú). El análisis se llevó a cabo utilizando dos metodologías: secuenciador automático (ABI 377 DNA sequencers®) y técnica de tinción con nitrato de plata. Los microsatélites fueron amplificados en tres reacciones de PCR múltiple y diez reacciones de PCR simple. El número de alelos varió entre 4 y 20. Las frecuencias alélicas y la probabilidad de exclusión (PE) fueron calculadas utilizando el software Cervus 2.0. Todos los loci, a excepción de dos, se encontraron dentro de los rangos encontrados en la literatura. La probabilidad de exclusión acumulada para los 10 loci fue 0.9999. La probabilidad de exclusión acumulada para cada reacción de PCR múltiple fue mayor a 0.90. Ambas metodologías obtuvieron los mismos resultados. Los resultados confirmaron la paternidad en 18 casos; sin embargo en el 22% de los casos (n=4) se identificaron padres alternativos que no correspondieron a los padres registrados., Ten polymorphic microsatellites for alpacas and llamas were used to evaluate paternity in 47 alpacas (18 crias, 18 mothers and 11 fathers) registered at IVITA-Maranganí Research Station, Canchis Province (Cusco-Peru). Analysis was carried out using two methodologies: Automatic Sequencer (ABI 377 DNA sequencers®) and silver staining techniques. Microsatellites were amplified in three multiple and ten single PCR reactions. The number of alleles varied between 4 and 20. The allelic frequencies and the exclusion probability were calculated using Cervus 2.0. All loci, except for two, were within the range published elsewhere. The accumulated exclusion probability for the ten loci was 0.9999. For each multiplex reaction the accumulated exclusion probability was more than 0.90. Both methodologies yielded the same results. The results confirmed paternity in 18 cases of parent-cria pairs, however in 22% of cases (n=4) were identified alternative parents than those indicated in farm records.

We report the first molecular evolutionary analysis of the family Camelidae by analysing the full DNA sequence of the mitochondrial cytochrome b gene. Estimates for the time of divergence of the Old World (Camelini) and New World (Lamini) tribes obtained from sequence data are in agreement with those derived from the fossil record. The DNA sequence data were also used to test current hypotheses concerning the ancestors of the domesticated llama and alpaca. The results show that hybridization has occurred in the ancestry of both domesticated camelids, obscuring the origin of the domestic species.

In the High Andes, much of the traditional knowledge related to natural resource utilization and agricultural practices was lost with the arrival of the Spaniards. That which has survived has been transformed, together with the landscape. Based upon ongoing research on the paleoecology and archaeology of the central High Andes, this paper presents the first results of pollen analysis from late-Pleistocene/early Holocene Laguna Seca (latitude 18011' South, longitude 69014'30" West) in the Chungara- Cotacotani lake district of Lauca National Park. The Laguna Seca record covers two crucial events in the evolution of high Andean puna ecosystems: (1) the period preceding the earliest human occupation, and (2) the period of change from a food-gathering to a food-producing economy. The results, though preliminary, indicate the contribution that these studies can make to the management and preservation of fragile mountain ecosystems.