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The continental margin off the southeastern Brazilian coast is punctuated by a series of geological-geomorphological features, such as subsurface saline diapirs and pockmarks at the seafloor interface, which evidence the abundant presence of oil and gas in the region. In several of these sites, hydrocarbons can be naturally released into the water column, areas are cold seep areas. These are marked by the presence of oil- and gas-dependent ecosystems, where specific organisms are able to fix carbon from hydrocarbon chemosynthesis. In addition, light hydrocarbon fluid flow through the sediment may build up authigenic carbonates that can be further colonized by cold-water corals, generating large carbonate mounds over geological time, normally positioned at the border of these pockmark features. The present work reports on a multidisciplinary oceanographic cruise carried out in the Santos Basin, SW Atlantic, to seek, map, and collect geological, chemical, and biological data from different deep-sea habitats. The cruise occurred in November 2019 on the R/V Alpha Crucis of the Oceanographic Institute of the University of São Paulo (IOUSP). We intended to discover and detail different geomorphological features, characterize free-living and symbiotic microorganisms, determine the chemosynthetic rates in relation to heterotrophic microbial production, and characterize the fauna and study their ecological and evolutionary links within and across ocean basins. All discoveries made during the cruise and their respective results will be presented separately in several papers that comprise this special volume.

SnO particles were synthesized by an alkali-assisted hydrothermal and microwave methods. The aqueous-based reactions were carried out at pH ~ 8, under inert atmosphere (Ar). The reactions were taken under different times, and a full XRD structural analysis was made to evaluate the conversion from the Sn6O4(OH)4 intermediate to SnO particles. Williamson-Hall analysis showed that the size and strain of the SnO particles were time and route treatment dependent. Microwave heating yielded a single tetragonal SnO phase after 1 h of thermal treatment, and TEM images revealed spherical-shaped SnO nanoparticles with an average size of 9(1) nm. While by the hydrothermal treatment single SnO phase was obtained only after 4 hours, yielding non-uniform and elongated particles with sub-micrometric size. A dissolution-recrystallization process was taken into account as the mechanism for SnO particles formation, in which hydroxylated complexes, Sn2(OH)6−2, then condense to form the oxide. The time-shorting reaction provided by the microwave-assisted synthesis may be attributed to better heat distribution.

Humiria balsamifera (Aubl), commonly known as “mirim”, is a plant of the Humiriaceae family, which consists of 39 species divided between eight genera: Duckesia, Endopleura, Humiria, Humiriastrum, Hylocara, Sacoglottis, Schistostemon, and Vantenea. This study aimed to characterize H. balsamifera extracts by LC-MS/MS and evaluate their antimicrobial potential through in vitro and in vivo assays. The leaves and stem bark of H. balsamifera were collected and dried at room temperature and then ground in a knife mill. The extracts were prepared with organic solvents in order to increase the polarity index (hexane, ethyl acetate, and methanol). The antimicrobial effects of these extracts were evaluated against the following bacterial strains: Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 15313, Salmonella enterica Typhimurium ATCC 14028, and Staphylococcus aureus ATCC 6538. The best activity was observed in the ethyl acetate (EALE = 780 µg/mL), methanol (MLE = 780 µg/mL), and hexane (HLE = 1560 µg/mL) leaf extracts against S. aureus. Considering the results for both antimicrobial and antibiofilm activities, the EALE extract was chosen to proceed to the infection assays, which used Tenebrio molitor larvae. The EALE treatment was able to extend the average lifespan of the larvae (6.5 days) in comparison to S. aureus-infected larvae (1 day). Next, the samples were characterized by High-Performance Liquid Chromatography coupled to a mass spectrometer, allowing the identification of 11 substances, including seven flavonoids, substances whose antimicrobial activity is already well-reported in the literature. The number of bioactive compounds found in the chemical composition of H. balsamifera emphasizes its significance in both traditional medicine and scientific research that studies new treatments based on substances from the Brazilian flora.

The present work evaluated the influence of assemblies other than preheating in the welding process obtained by coated electrode, using high strength and low alloy steel SAR 80T as the base metal and the AWS E7018 electrode as the addition metal. In order to prevent cracks, preheating and interpassing for low alloy steel was performed. Depending on the way the preheating equipment is installed, it may take more or less time to reach the preheat temperature. Different assembly arrangements for top joints were evaluated, aiming at cost reduction, in addition to evaluations of the mechanical properties of the joint. Visual testing, ultrasound, micrography, macrography and cross-sectional traction were performed. The results obtained were considered acceptable and showed that there was an influence on time, cost of preheating and resistance to impact.

The Cerrado biome, predominant in the region of Sete Lagoas is marked by fruit species of the local flora, such as: Araticum, Cagaita, JatobAi, Mangaba, Pequi, among others that are not always known by the community. Environmental education is an important tool for awareness and preservation of the local biome. The extension program aims to bring the community knowledge of the biome and the typical fruit species of the region that are not known and recognized. Inducing a new look and expanding knowledge among students in order to highlight the biome and its characteristics in a playful and didactic way. The program was successful in interventions and achieved satisfactory results in relation to students' perception of classroom work, extolling the biome in which they live, generating new multipliers. The work done in an interdisciplinary way achieved an increase in the percentage of correct answers compared to the numbers obtained before the interventions.

The race structure and genotypic diversity of four Leptosphaeria maculans populations isolated from Brassica oleracea (broccoli, cauliflower, cabbage, etc.) in central Mexico (Aguascalientes, Guanajuato and Zacatecas states) were analysed. Race structure was characterized by an unusually low diversity at three locations out of four. Fourteen minisatellite markers revealed a high proportion of repeated multilocus genotypes in these populations, combined with a significant linkage disequilibrium and strong clonal fraction (65‐87%). The occurrence of the mating-type idiomorphs always significantly departed from the 1:1 proportion expected in the case of random mating. Each population thus consists of a few (four to nine) multilocus genotypes which are specific to each location. These data strongly support the hypothesis of exclusive, or a high rate of, clonal multiplication. Comparison of cropping practices between B. oleracea and B. napus indicate that the shift in reproductive behaviour of the fungus is chiefly man-mediated.

Portuguese perennial kale is a rare leafy vegetable and forage crop in Portugal. It may be a relic of a primitive kale form which could have been grown on a large scale in Portugal and in West Europe. This kale has strong branching habit with rosettes of leaves in the stems. It shows a strongly reduced flowering ability and for this reason is vegetatively propagated. In the past, perennial kale has probably been widely distributed in West Europe, but the crop has gradually declined in occurrence in this area. At present it is an almost extinct vegetable crop and by this reason deserves to be preserved.

Breeding is probably as old as agriculture itself. Early vegetable breeders developed landrace cultivars by selection of favorable variations in horticultural traits, yield and resistance to diseases and other problems. Later new breeding methods were developed, including hybridization techniques, culminating with the use of recently developed molecular tools, all leading to our modern improved vegetable cultivars. Great emphasis on protection of cultivars by seed companies, including development of F1 hybrids, plant cultivar protection and patenting have been done. There were 392 vegetable crops cultivated worldwide but only slightly over one half of the total number of them have attracted commercial breeding attention. In recent times, there have been challenges and new trends in the breeding domain. These include an unrelenting movement away from well supported public breeding institutions to a breeding world dominated by private entities, and an increase in size of the companies in the private sector, with emphasis on the major vegetable crops. Almost half of the world vegetable farmers are poor and cannot afford to buy hybrid seed every growing season. Their economics and logistics make them difficult to buy expensive improved or hybrid cultivars since the lack of capital by subsistence farmers denies them the opportunity to invest in vegetable production inputs. If credit facilities and other input facilities are offered, improved or hybrid cultivars can have also a great impact on subsistence vegetable farmers overcoming their poverty and food insecurity. In last 50 years vegetable genetic resources are being lost, on a global scale at the rate of 1–2% per year. The multinational seed companies concentration in huge corporations have merged or canceled some vegetable breeding programs to reduce costs. Then there will be fewer vegetable breeders in the future and the growers will be dependent on a narrower genetic background, that could contribute in a near future, for food insecurity. Smaller seed companies, which are usually specialize in few vegetable crops, must be supported, possibly through autonomous affiliation with the larger companies. There is a need of investment in research breeding and cultivar development in traditionally open-pollinated cultivars and in the minor and so-called “forgotten” vegetables. More investments in this area will mean cheaper cultivars for growers to choose from and more preservation of vegetable biodiversity. In recent years, private plant breeding programs have increased in size and number. Financial investment also increased, as well as interest in intellectual property protection. Protective measures, especially patenting, must be moderated to eliminate coverage so broad that it stifles innovation. The intellectual property protection laws for plants must be made less restrictive to encourage research and free flow of materials and information. Public sector breeding must remains vigorous, especially in areas where the private sector does not function. This will often require benevolent public/private partnerships as well as government support. Intellectual property rights laws for plants must be made less restrictive to encourage freer flow of materials. Active and positive connections between the private and public breeding sectors and large-scale gene banks are required to avoid a possible conflict involving breeders’ rights, gene preservation and erosion. Improved and hybrid vegetable cultivars are, and will continue to be, the most effective, environmentally safe, and sustainable way to ensure global food security in the future.

The identification of seedling resistance to white rust of crucifers was performed in a screening of a B. oleracea core collection with 400 accessions representing the genetic and geographic diversity of the species. Fifty seedlings per accession were tested against the Portuguese isolate Ac502 using the methodology and evaluation procedures developed by and . The percentage of resistant seedlings (%R) and the conventional rating criteria of the mean Disease Index (DI) based on the two different evaluation procedures of disease expression used, were compared and adopted as the criteria to rank the accessions for their interest as sources of resistance. A great variability of reactions was found between and within accessions of the core collection, ranging from complete resistance to full susceptibility. Sources of resistance were found namely among the cauliflowers, broccoli and tronchuda cabbages gene pools. Forty-seven accessions presented at least 20% of resistant seedlings. Nine accessions (the kales INRA18 and INRA62, the cauliflowers HRI4856, HRI4866 and HRI5424, the loose-head cabbage HRI11555, the savoy cabbage BRA848, the black broccoli HRI6318 and the Portuguese tronchuda cabbage ISA207) presented 50–78% of resistant seedlings and so they should be considered as potential and useful sources for direct use in breeding programs for white rust resistance. Fourteen inbred lines, representing the full range of disease expression, derived from resistant accessions of the core collection were also tested for resistance to other two Portuguese isolates (Ac503 and Ac504) and to a UK isolate. The results provided no evidence of differential reaction to the A. candida isolates tested.

Conditions for reliable induction of embryogenesis from isolated microspores were studied in ten genotypes of broccoli. Embryo yields were significantly increased in almost all of the broccoli genotypes by the incubation at 32.5 °C for 1 day, than when the standard incubation at 30 °C for 2 days was used. Treatments of 48 hours at 32.5 °C produced less than optimal results suggesting that broccoli microspores are more sensitive to high temperatures than those of B. napus. The use of the 1/2 NLN-13 medium yielded greater number of embryos than the standard NLN-13. The magnitude of the response to the redution of the concentration of major salts by half in the NLN medium varied with the different genotypes. High embryogenic broccoli cultivars, such as ‘Shogun’, ‘SDB9’, and ‘Green Valiant’, presented a better response to the reduction of the concentration of major salts by half in NLN-13. Reduction never produced a detrimental effect on embryo yield and seems not to have any effect in the subsequent development of embryos in plants.

The effect of the addition of a 0.1 ml drop of activated charcoal (AC) on microspore culture embryogenesis was studied in nine morphotypes of Brassica oleracea. Embryo yields were significantly increased in all of the morphotypes by the addition of the 0.1 ml drop of AC to the microspore culture media. The magnitude of the response to the addition of AC varied with the different plants and morphotypes. The addition of AC never produced a detrimental effect. A qualitative improvement of the subsequent development of embryos to plants was also observed with the addition of AC. Data suggest that the addition of AC to microspore culture media promoted embryo production in different B. oleracea morphotypes.

Portuguese coles, tronchuda cabbage and galega kale landraces, are a unique group of vegetables very important for Portuguese horticulture owing to their excellent adaptation to prevalecent climatic conditions and good integration in the traditional small farming cropping systems. Reports on these coles are scarce and scattered. A bibliography review is presented on some general aspects of Portuguese cole landraces such as botany, folknames, foreign synonymy, origin, history, and worldwide spreading. In addition to such available information, other data has been gathered from growers in door-to-door enquiries during a germplasm collecting of Portuguese coles. Recent research on the taxonomy of Portuguese coles has been summarized.

Isozyme analysis was used to study the genetic variation and the genetic relationships of a collection of 48 Brassica spp. including 31 Portuguese coles (Brassica oleracea L.) accessions representative of the different landraces cultivated in Portugal. Other brassicas included in this experiment were Jersey kale, kailaan, common cabbages, broccolis, cauliflower, nine-chromosome wild brassica and turnip. Nine enzymes used in the starch gel electrophoresis included: PGM, PGI, AAT, LAP, TPI, FBP, SOD, IDH and GR. Twenty-one putative loci were revealed, with 3 showing invariance and the other 18 contained 50 alleles. The allelic frequencies at these loci represented by 40 plants per accession were used to calculate the following estimators of genetic variation: % of polymorphic loci, average number of alleles per polymorphic loci, average number of alleles per locus, and index of heterozygosity. The genetic relationships were evaluated considering Nei (1978) and Rogers (1972) genetic distances between each pair of accessions whose matrices were hierarchically clustered by the UPGMA method. The accessions were also studied using the principal coordinate analysis. Portuguese Tronchuda cabbages and Galega kales have shown high genetic diversity in comparison with the other accessions. This indicates their potential variation for use in breeding programs. The UPGMA results show that the 48 accessions, with the exception of B. insularis, B. cretica, and turnip, can be clustered into 6 groups: (a) Portuguese Tronchuda cabbages, Galega kales and Algarve cabbages; (b) common cabbages and kales; (c) Couve Poda do Algarve and broccoli; (d) Algarve cabbage and common cabbages; (e) kailaan; (f) broccoli and cauliflower. The groupings obtained by the isozyme analysis are difficult to interpret considering the origin of the Brassica spp. and the morphological resemblance among the accessions.

Morphological characters, nuclear RFLPs, and isozyme analysis were used to study the similarity between 32 Portuguese Tronchuda cabbage and Galega kale landraces, and some cabbage cultivars traditionally grown in Portugal. Forty-six morphological characters observed in two consecutive years, RFLP data from 55 nuclear probes, detecting 291 polymorphic nuclear DNA restriction fragments, and allelic frequencies in 21 putative loci, generated by nine isozymes, were analyzed by the unweighted pair group method, using arithmetic averages (UPGMA), in order to present the results in the form of a phenogram. The three methods resulted in different clustering patterns of the 32 cole accessions. Morphological characters gave consistent clustering according to the traditional landrace definition and denomination, producing clear separation between Tronchuda cabbages and Galega kales. RFLPs were unable to separate Tronchuda cabbages from Galega kales and defined five landrace groups corresponding to their geographic origins rather than to their morphological similarities. Isozymes showed poor accession discrimination and an intermediate clustering pattern with some accessions being clustered according to their geographic origins and others according to their morphological similarities. Portuguese Tronchuda cabbages and Galega kales constitute a distinct and relatively homogenous group within Brassica oleracea, sharing the same genetic background. It is concluded that Portuguese coles have evolved independently from a common ancestor to the present cultivated forms. Portugal should be considered as an important region of domestication of specialized leafy coles.

Forty-four landraces of Portuguese coles (Brassica oleracea L.), representing different morphotypes, were screened at the cotyledon stage for downy mildew resistance, caused by Peronospora parasitica. Two isolates of the pathogen, PHW 630 and PHW 828, were used in the experiment. The mean disease interaction phenotype varied among the landraces. Overall, the Portuguese coles have shown resistance to both isolates, particularly to isolate PHW 828. Some landraces were identified with differential reaction to the two P. parasitica isolates. Some of the Portuguese coles are, therefore, good sources of resistance genes to P. parasitica, and could be used in breeding programs or in the development of a downy mildew host differential set. Among the morphotypes tested, Couve Algarvia (ISA 207), Couve Galega (ISA 243 and 187) and Couve Gloria de Portugal (ISA 84) have presented the highest resistance interaction. For the development of host differential sets, the landraces ISA 2 (Penca de Mirandela), ISA 35 (Couve de Valhascos) and ISA 600 (Penca Espanhola) could be further selected for the differentiation of P. parasitica isolates. They are resistant to PHW 828 and susceptible to PHW 643. The same morphotypes and landraces were also screened at the seedling stage for resistance to clubroot, caused by Plasmodiophora brassicae Woron.. Although some variation for resistance could be noticed in some the landraces tested, mainly Galega kale types, none of them presented a sufficient level of resistance as to be directly be useful in breeding for resistance to race 6 of P. brassicae.

Summary Fifty-six landraces of Portuguese coles (Brassica oleracea L.),representing fourteen morphotypes, were screened for resistance to Leptosphaeria maculansat the cotyledon stage . Three isolates of the pathogen belonging to three pathogenicity groups were used in the screening . The mean disease interaction phenotype of theB. oleracea landraces with the L. maculansisolates confirmed their differences in pathogenicity . When compared to pathogenicity grouping using a B. napus differential set, a different classification was observed on B. oleracea for the three L. maculansisolates, suggesting possible differences in the genetic interaction between the two plant species and the pathogen . Most of the Portuguese cole landraces were found to be susceptible to all three isolates of L. maculans,although a few have shown intermediate interaction pheno

RFLPs were used to study the genetic relationships of 31 Portuguese coles (Brassica oleracea L.) representing the different ecotypes or landraces grown in Portugal. Other cole crops such as Jersey kale, Chinese kales, common cabbages, broccolis and cauliflower, two nine-chromosome wild species, and one accession of turnip also were included to determine the evolutionary relationships of Portuguese coles to other related coles. Principal coordinates and cluster analyses were conducted using a package of computer programs and RFLP data from 55 nuclear DNA probes, detecting 291 polymorphic restriction fragments. The results showed that the 48 accessions clustered into five groups: (a) a dispersed group with turnip and the wild brassicas; (b) Chinese kales; (c) broccolis and cauliflower; (d) a disperse group including Algarve and Jersey kales, thousand head kale, cabbages, and savoy cabbage; (e) a large and compact group containing all Portuguese tronchuda cabbages and kales. This unique and closely related group containing Portuguese tronchuda cabbages and kales could be further divided into five subgroups corresponding to the major areas of cultivation. The phenetic groupings of Portuguese tronchuda cabbages and kales based on RFLP data corresponded more with their geographic origins (collecting sites) than with their morphological similarities. A mechanism involving geographic isolation and frequent intercrossing within local areas is proposed to explain genetic relationships among Portuguese tronchuda cabbages and kales.

A medical audit on the prenatal care program in the Vila Municipal Health Center, Pelotas, RS, Brazil, was described with the purpose of verifying the aspects of the medical process and improving the program's effectiveness.Data from prenatal specific records were collected. Pregnant women with delivery due date in 1997 and in the first semester of 1998 were included in the study. Women registered in the program when they were 4-month pregnant and who had had at least 5 visits were also enrolled. Bivariate analysis was used to detect health care indicators.A total of 73 pregnant women were registered in the program in 1997 and 75 in 1998. In 1997, the average number of medical visits was 5.2, while in 1998 this average was 6.2. The difference between the means was statistically significant (p0.05). Some medical process indicators were analysed to verify the quality of the care.The use of the epidemiological method to organize health services was discussed. This type of study requires few resources and time and it can provide guidelines to health service actions.

The objective was determine the use of health services by the adult population in Pelotas, RS, Brazil.A cross-sectional study was made on the basis of a population sample. One thousand six hundred and fifty-seven persons we interviewed during the months of March and June, 1992. A percentage of 9.7 of the sample was lost.Two dependents variables. One the type of service as determined by type of payment. The other the number of medical visits made during the previous year. The type of service was seen to be associated with the following social variables: social class, level of schooling and place of residence. The frequency of medical visits was associated with sex, risk factors and reasons for the visit.It was concluded that choice of the type of service depends more heavily in social class than other variables associated with the severity of the disease in question.

Twelve accessions of tronchuda cabbage landraces were tested for their ability to produce embryos through microspore culture in NLN-13 medium. A sample from all the isolations was stained with DAPI for determination of microspore developmental stage. The relationship between the microspore developmental stage and the production of microspore-derived embryos was evaluated.\ud Embryos were obtained from all the accessions. Considerable variation was observed between isolations with different developmental stage of microspores, accessions and plants within the same accession. The best embryogenic responses occurred in microspore populations with approximately 10 to 89% of binucleate pollen. The highest embryo yield was obtained with one of the ‘Couve de Valhascos’ accessions (0.41 embryos / 1 000 microspores) and the lowest yields with the accessions of ‘Couve Portuguesa’ (less than 0.07 embryos / 1 000 microspores).

An animation of a deformable surface with fixed topology is formed by a set of connected points (particles), which follow a trajectory over time. The transmission of such an animation is a task that consumes large amounts of bandwidth, as the position of each particle, in each time instant, needs to be transmitted. We propose a wavelet-based compression and streaming mechanism that allows the minimization of the size of the animation data, hence decreasing the total transmission time. The proposed transmission scheme, based on the time-localized feature of the wavelet transform, will be able to stream the animation so that a receiver can immediately view the fraction of the animation received while wavelet coefficients are still arriving. info:eu-repo/semantics/publishedVersion