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F stars lie in the transition region between cool stars with dynamo generated fields and hot star fossil fields and offer an interesting window into the generation of magnetic fields in shallow convection zones. In this paper, we investigate the magnetic field of the mature F7V primary of the bright χ Draconis system. χ Dra was observed in circularly polarized light at four epochs from 2014 through to 2019 using the NARVAL spectropolarimeter at the Téléscope Bernard Lyot. Using the technique of least-squares deconvolution, we created high signal-to-noise line profiles from which we were able to measure the radial velocity of both the primary and secondary and use these to improve the orbital parameters of the system. Additionally, we used the technique of Zeeman Doppler imaging to reconstruct the large-scale magnetic field geometry of the primary at the four epochs. The magnetic maps show that χ Dra A has a predominately dipolar poloidal magnetic field at all epochs with the magnetic axis well aligned with the stellar rotation axis. The large-scale features of the magnetic field appear to be relatively stable over the ∼5 yr time base of our observations, with no evidence of any polarity reversals. We used the magnetic field maps to model the wind from χ Dra A at all epochs, showing that the mass-loss from the stellar wind of χ Dra A is 3–6 times the current solar value, while the angular momentum loss from the wind is around 3–4 times the solar value.

Integrated photonics has profoundly affected a wide range of technologies underpinning modern society1–4. The ability to fabricate a complete optical system on a chip offers unrivalled scalability, weight, cost and power efficiency5,6. Over the last decade, the progression from pure III–V materials platforms to silicon photonics has significantly broadened the scope of integrated photonics, by combining integrated lasers with the high-volume, advanced fabrication capabilities of the commercial electronics industry7,8. Yet, despite remarkable manufacturing advantages, reliance on silicon-based waveguides currently limits the spectral window available to photonic integrated circuits (PICs). Here, we present a new generation of integrated photonics by directly uniting III–V materials with silicon nitride waveguides on Si wafers. Using this technology, we present a fully integrated PIC at photon energies greater than the bandgap of silicon, demonstrating essential photonic building blocks, including lasers, amplifiers, photodetectors, modulators and passives, all operating at submicrometre wavelengths. Using this platform, we achieve unprecedented coherence and tunability in an integrated laser at short wavelength. Furthermore, by making use of this higher photon energy, we demonstrate superb high-temperature performance and kHz-level fundamental linewidths at elevated temperatures. Given the many potential applications at short wavelengths, the success of this integration strategy unlocks a broad range of new integrated photonics applications.

There is a need to achieve sustainable agricultural production to secure food, fiber, and fuel for a growing global population. Climate-smart (CS) actions (no-till and cover crops) can reduce carbon emissions and promote soil organic carbon (SOC) storage. Contemporary voluntary carbon markets provide producers with a monetary incentive to adopt CS actions. However, SOC–yield dynamics under CS actions are not well known, making it difficult for producers to judge whether additional income from carbon credits will offset potential losses to yield income. We designed a SOC–yield framework that captures SOC–yield–income dynamics under traditional (reduced tillage, no cover crops) and CS actions. Using a modified structured decision-making approach, we applied the framework to a case study in which producers aim to increase income by selling carbon credits after adopting CS actions. Specifically, we demonstrated how to balance tradeoffs between yield and carbon credit income that arise from tillage and winter cover crop actions (cereal rye, Secale cereale L. and crimson clover, Trifolium incarnatum L.) in a soybean (Glycine max L.) production system in Mississippi. Results indicated that a producer could minimize losses to net yield income by adopting no-till if already using cover crops. There was also evidence that carbon credit income could offset losses to yield income when adopting CS in place of traditional actions. Identifying risks to yield income and SOC storage can help design carbon neutrality policies that have minimum impact on a producer’s income. History: This paper has been accepted for the Decision Analysis Special Issue on Further Environmental Sustainability. Funding: This work was supported by the USDA-ARS [Grants 58-0200-0-002 (Advancing Agricultural Research) and 58-6001-8-003] and the USDA National Institute of Food and Agriculture [McIntire Stennis Project 1020959]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/deca.2023.0478 .

Paired Box 8 (PAX8) is a lineage-specific transcription factor that has essential roles during embryogenesis and tumorigenesis. The importance of PAX8 in the development of the reproductive system is highlighted by abnormalities observed upon the loss or mutation of this PAX family member. In cancer, PAX8 expression is deregulated in a key set of neoplasms, including those arising from the Müllerian ducts. The roles of PAX8 in oncogenesis are diverse and include epigenetic remodeling, stimulation of proliferation, inhibition of apoptosis, and regulation of angiogenesis. PAX8 can interact with different protein partners during cancer progression and may exhibit significant function-altering alternative splicing. Moreover, expression of PAX8 in cancer can also serve as a biomarker for diagnostic and prognostic purposes. In this review, we focus on the roles of PAX8 in cancers of the reproductive system. Understanding the diverse mechanisms of action of PAX8 in development and oncogenesis may identify new vulnerabilities in malignancies that currently lack effective therapies.

Supplementary Figure 1 from Unraveling the Mysteries of PAX8 in Reproductive Tract Cancers

Supplementary Table 1, Figures 1-7 from Jagged-1 and Notch3 Juxtacrine Loop Regulates Ovarian Tumor Growth and Adhesion

Notch3 gene amplification and pathway activation have been reported in ovarian serous carcinoma. However, the primary Notch3 ligand that initiates signal transduction in ovarian cancer remains unclear. In this report, we identify Jagged-1 as the highest expressed Notch ligand in ovarian tumor cells as well as in peritoneal mesothelial cells that are in direct contact with disseminated ovarian cancer cells. Cell-cell adhesion and cellular proliferation were reduced in Notch3-expressing ovarian cancer cells that were cocultured with Jagged-1 knockdown mesothelial and tumor feeder cells. Interaction of Notch3-expressing ovarian cancer cells with Jagged-1–expressing feeder cells activated the promoter activity of candidate Notch3 target genes, and this activity was attenuated by Notch3 siRNA. Constitutive expression of the Notch3 intracellular domain significantly suppressed the Jagged-1 shRNA–mediated growth inhibitory effect. In Notch3-expressing ovarian cancer cells, Jagged-1–stimulating peptides enhanced cellular proliferation, which was suppressed by γ-secretase inhibitor and Notch3 siRNA. Taken together, our results show that Jagged-1 is the primary Notch3 ligand in ovarian carcinoma and Jagged-1/Notch3 interaction constitutes a juxtacrine loop promoting proliferation and dissemination of ovarian cancer cells within the intraperitoneal cavity. [Cancer Res 2008;68(14):5716–23]

Reverse takotsubo cardiomyopathy (rTCM) is characterized by basal ballooning and accounts for approximately 1% of all TCM. To our knowledge, there have been no reports describing rTCM complicated by acute, severe, transient mitral regurgitation (MR). A 75-year-old woman with a medical history of hypertension, dyslipidemia, and anxiety presented to the hospital with 2 days of substernal chest pain, dyspnea, and nausea. Initial troponin was 0.203 ng/mL, and electrocardiography showed sinus tachycardia at 121 bpm, with inferior and anterolateral ST segment depressions. Transthoracic echocardiogram (TTE) found an ejection fraction of 30%, apical hyperkinesis, severe hypokinesis of the basal to mid segments of the left ventricle (LV), and a severe central MR jet. Cardiac angiography demonstrated non-obstructive coronary artery disease, and elevated left ventricular end diastolic pressures. Left ventriculography showed a hyperdynamic apex and severe basal hypokinesis. The patient was treated medically, clinical status improved, and was discharged on day 3. TTE four weeks later, showed an ejection fraction of 60–65%, mild MR, and normal LV function. rTCM is the rarest variant of TCM. Basal and mid-myocardial stunning can cause severe secondary MR leading to acute congestive heart failure, mimicking acute coronary syndrome with acute MR. rTCM with rapidly reversible severe MR has not previously been described. Learning Objectives Mitral regurgitation secondary to reverse takotsubo cardiomyopathy can mimic coronary flow obstruction syndromes and when recognized early, can improve with amelioration of cardiomyopathy. Acute severe mitral regurgitation in reverse takotsubo cardiomyopathy is likely secondary to basilar dilatation. Reverse takotsubo cardiomyopathy represents approximately 1% of all takotsubo cardiomyopathy cases.

AimsAssess bacterial community changes over time in soybean (Glycine max) crop fields following cover crop (CC) and no-till (NT) implementation under natural abiotic stressors.Method and resultsSoil bacterial community composition was obtained by amplifying, sequencing, and analysing the V4 region of the 16S rRNA gene. Generalized linear mixed models were used to assess the effects of tillage, CC, and time on bacterial community response. The most abundant phyla present were Acidobacteria, Actinobacteria, Bacteroidetes, and Verrucomicrobia. Bacterial diversity increased in periods with abundant water. Reduced tillage (RT) increased overall bacterial diversity, but NT with a CC was not significantly different than RT treatments under drought conditions. CCs shifted abundances of Firmicutes and Bacteroidetes depending on abiotic conditions.ConclusionsIn the Lower Mississippi Alluvial Valley (LMAV), USA, NT practices lower diversity and influence long-term community changes while cover crops enact a seasonal response to environmental conditions. NT and RT management affect soil bacterial communities differently than found in other regions of the country.

Supplemental Digital Content is Available in the Text.

The transition of natural landscapes to agricultural uses has resulted in severe loss of soil organic carbon, significantly contributing to CO2 emissions and rising global temperatures. However, soil has the largest store of terrestrial carbon (C), a considerable sink and effective strategy for climate change mitigation if managed properly. Cover crops (CC) and no-till (NT) management are two management strategies that are known to increase percent organic carbon (%OC); however, adoption of these practices has been low in the mid-South due to lack of region-specific research and resistance to unproven practices. Therefore, the purpose of this study was to evaluate the impacts of CC-NT treatments in soybean cropping systems on soil percent organic carbon (%OC) and CO2 flux following long-term implementation. Results showed significantly greater %OC in NT (1.27% ± 0.03) than reduced till (RT; 1.10% ± 0.03; p < 0.001) and greater in both CC (rye: 1.23% ± 0.03, rye + clover: 1.22% ± 0.03) than no cover (1.11% ± 0.03; p < 0.001). Bacterial abundance (p = 0.005) and pH (p = 0.006) were significant predictors of %OC. There was no overall significant difference in CO2 flux between tillage or CC treatments; however, there were significant differences between NT and RT in July of 2020 when %RH increased (p < 0.001). Bacterial abundance negatively impacted CO2 flux (p < 0.05), which contradicts most studies. The rate of proportional change and pattern of variability in C pools suggested loss of %OC in RT treatments that were not apparent when considering %OC alone. The results of this study provide valuable insight into C turnover and the effectiveness of CC use in the Mid-South to increase soil C stocks.

Historical contingency has long figured prominently in the conceptual frameworks of evolutionary biology and community ecology. Evolutionary biologists typically consider the effects of chance mutation and historical contingency in driving divergence and convergence of traits in populations, whereas ecologists instead are often interested in the role of historical contingency in community assembly and succession. Although genetic differences among individuals in populations can influence community interactions, variability among populations of the same species has received relatively little attention for its potential role in community assembly and succession. We used a community-level study of experimental evolution in two compositionally different assemblages of protists and rotifers to explore whether initial differences in species abundances among communities attributed to differences in evolutionary history, persisted as species that continued to evolve over time. In each assemblage, we observed significant convergence between two invaded treatments initially differing in evolutionary history over an observation period equal to ~40-80 generations for most species. Nonetheless, community structure failed to converge completely across all invaded treatments within an assemblage to a single structure. This suggests that whereas the species in the assemblage represent a common selective regime, differences in populations reflecting their evolutionary history can produce long-lasting transient alternative community states. In one assemblage, we also observed increasing within-treatment variability among replicate communities over time, suggesting that ecological drift may be another factor contributing to community change. Although subtle, these transient alternative states, in which communities differed in the abundance of interacting species, could nonetheless have important functional consequences, suggesting that the role of evolution in driving these states deserves greater attention.

The stellar activity of M dwarfs is the main limitation for discovering and characterizing exoplanets orbiting them since it induces quasi-periodic RV variations. We aim to characterize the magnetic field and stellar activity of the early, moderately active, M dwarf Gl205 in the optical and nIR domains. We obtained high-precision quasi-simultaneous spectra in the optical and nIR with the SOPHIE spectrograph and SPIRou spectropolarimeter between 2019 and 2022. We computed the RVs from both instruments and the SPIRou Stokes V profiles. We used ZDI to map the large-scale magnetic field over the time span of the observations. We studied the temporal behavior of optical and nIR RVs and activity indicators with the Lomb-Scargle periodogram and a quasi-periodic GP regression. In the nIR, we studied the equivalent width of Al I, Ti I, K I, Fe I, and He I. We modeled the activity-induced RV jitter using a multi-dimensional GP regression with activity indicators as ancillary time series. The optical and nIR RVs have similar scatter but nIR shows a more complex temporal evolution. We observe an evolution of the magnetic field topology from a poloidal dipolar field in 2019 to a dominantly toroidal field in 2022. We measured a stellar rotation period of Prot=34.4$\pm$0.5 d in the longitudinal magnetic field. Using ZDI we measure the amount of latitudinal differential rotation (DR) shearing the stellar surface yielding rotation periods of Peq=32.0$\pm$1.8 d at the stellar equator and Ppol=45.5$\pm$0.3 d at the poles. We observed inconsistencies in the activity indicators' periodicities that could be explained by these DR values. The multi-dimensional GP modeling yields an RMS of the RV residuals down to the noise level of 3 m/s for both instruments, using as ancillary time series H$\alpha$ and the BIS in the optical, and the FWHM in the nIR., Comment: 41 pages, 24 figures. Accepted for publication in A&A. Improved quality of figures and reduced size of Appendix

Context. The rotation period of stars is an important parameter together with mass, radius, and effective temperature. It is an essential parameter for any radial velocity monitoring, as stellar activity can mimic the presence of a planet at the stellar rotation period. Several methods exist to measure it, including long sequences of photometric measurements or temporal series of stellar activity indicators. Aims. Here, we use the circular polarization in near-infrared spectral lines for a sample of 43 quiet M dwarfs and compare the measured rotation periods to those obtained with other methods. Methods. From Stokes V spectropolarimetric sequences observed with SPIRou at the Canada-France-Hawaii Telescope and the data processed with the APERO pipeline, we computed the least-squares deconvolution profiles using different masks of atomic stellar lines with known Landé factor appropriate to the effective temperature of the star. We derived the longitudinal magnetic field to examine its possible variation in 50 to 200 observations of each star. To determine the stellar rotation period, we applied a Gaussian process regression, enabling us to determine the rotation period of stars with evolving longitudinal field. Results. We were able to measure a rotation period for 27 of the 43 stars of our sample. The rotation period was previously unknown for 8 of these stars. Our rotation periods agree well with periods found in the literature based on photometry and activity indicators, and we confirm that near-infrared spectropolarimetry is an important tool for measuring rotation periods, even for magnetically quiet stars. Furthermore, we computed the ages for 20 stars of our sample using gyrochronology.

Paired Box 8 (PAX8) is a lineage-specific transcription factor that has essential roles during embryogenesis and tumorigenesis. The importance of PAX8 in the development of the reproductive system is highlighted by abnormalities observed upon the loss or mutation of this PAX family member. In cancer, PAX8 expression is deregulated in a key set of neoplasms, including those arising from the Müllerian ducts. The roles of PAX8 in oncogenesis are diverse and include epigenetic remodeling, stimulation of proliferation, inhibition of apoptosis, and regulation of angiogenesis. PAX8 can interact with different protein partners during cancer progression and may exhibit significant function-altering alternative splicing. Moreover, expression of PAX8 in cancer can also serve as a biomarker for diagnostic and prognostic purposes. In this review, we focus on the roles of PAX8 in cancers of the reproductive system. Understanding the diverse mechanisms of action of PAX8 in development and oncogenesis may identify new vulnerabilities in malignancies that currently lack effective therapies.

In polyelectrolyte complex coacervates, changes in salt concentration and changes in polymer concentration are typically strongly coupled, complicating interpretation of the salt- and polymer-concentration-dependent dynamics of these materials. To address this problem, we developed a “salt addition” method for preparation of complex coacervates that allows the salt concentration of a coacervate sample to be varied without changing its polymer concentration. This method was used to prepare coacervates of poly(styrene sulfonate) (PSS) with poly(diallyldimethylammonium chloride) (PDADMAC) with salt concentrations between 1.2 and 2 M and volume fractions of polymer between 0.1 and 0.25. Characterization of these samples by small-amplitude oscillatory shear rheology revealed that the relaxation times scale significantly more strongly with polymer volume fraction than has been previously assumed, highlighting the need to account for both salt and polymer-dependent contributions to the dynamics of these complex materials.

Spectropolarimetric data allow for simultaneous monitoring of stellar chromospheric $\log{R^{\prime}_{\rm{HK}}}$ activity and the surface-averaged longitudinal magnetic field, $B_l$, giving the opportunity to probe the relationship between large-scale stellar magnetic fields and chromospheric manifestations of magnetism. We present $\log{R^{\prime}_{\rm{HK}}}$ and/or $B_l$ measurements for 954 mid-F to mid-M stars derived from spectropolarimetric observations contained within the PolarBase database. Our magnetically active sample complements previous stellar activity surveys that focus on inactive planet-search targets. We find a positive correlation between mean $\log{R^{\prime}_{\rm{HK}}}$ and mean $\log|B_l|$, but for G stars the relationship may undergo a change between $\log{R'_{\rm{HK}}}\sim-4.4$ and $-4.8$. The mean $\log{R^{\prime}_{\rm{HK}}}$ shows a similar change with respect to the $\log{R^{\prime}_{\rm{HK}}}$ variability amplitude for intermediately-active G stars. We also combine our results with archival chromospheric activity data and published observations of large-scale magnetic field geometries derived using Zeeman Doppler Imaging. The chromospheric activity data indicate a slight under-density of late-F to early-K stars with $-4.75\leq\log{R'_{\rm HK}}\leq-4.5$. This is not as prominent as the original Vaughan-Preston gap, and we do not detect similar under-populated regions in the distributions of the mean $|B_l|$, or the $B_l$ and $\log{R'_{\rm HK}}$ variability amplitudes. Chromospheric activity, activity variability and toroidal field strength decrease on the main sequence as rotation slows. For G stars, the disappearance of dominant toroidal fields occurs at a similar chromospheric activity level as the change in the relationships between chromospheric activity, activity variability and mean field strength., 20 pages, 15 figures, 3 tables

PAX8 is a master transcription factor that is essential during embryogenesis and promotes neoplastic growth. It is expressed by the secretory cells lining the female reproductive tract, and its deletion during development results in atresia of reproductive tract organs. Nearly all ovarian carcinomas express PAX8, and its knockdown results in apoptosis of ovarian cancer cells. To explore the role of PAX8 in these tissues, we purified the PAX8 protein complex from nonmalignant fallopian tube cells and high-grade serous ovarian carcinoma cell lines. We found that PAX8 was a member of a large chromatin remodeling complex and preferentially interacted with SOX17, another developmental transcription factor. Depleting either PAX8 or SOX17 from cancer cells altered the expression of factors involved in angiogenesis and functionally disrupted tubule and capillary formation in cell culture and mouse models. PAX8 and SOX17 in ovarian cancer cells promoted the secretion of angiogenic factors by suppressing the expression of SERPINE1 , which encodes a proteinase inhibitor with anti a ngiogenic effects. The findings reveal a non–cell-autonomous function of these transcription factors in regulating angiogenesis in ovarian cancer.

STUDY QUESTION Is poor ovarian response associated with a change in predicted age based on a DNA methylation-derived age prediction model (the Horvath algorithm) in white blood cells (WBCs) or cumulus cells (CCs)? SUMMARY ANSWER In young women, poor ovarian response is associated with epigenetic age acceleration within WBC samples but is not associated with age-related changes in CC. WHAT IS KNOWN ALREADY The majority of human tissues follow predictable patterns of methylation which can be assessed throughout a person’s lifetime. DNA methylation patterns may serve as informative biomarkers of aging within various tissues. Horvath’s ‘epigenetic clock’, which is a DNA methylation-derived age prediction model, accurately predicts a subject’s true chronologic age when applied to WBC but not to CC. STUDY DESIGN, SIZE, DURATION A prospective cohort study was carried out involving 175 women undergoing ovarian stimulation between February 2017 and December 2018. Women were grouped according to a poor (≤5 oocytes retrieved) or good (>5 oocytes) response to ovarian stimulation. Those with polycystic ovary syndrome (PCOS) (n = 35) were placed in the good responder group. PARTICIPANTS/MATERIALS, SETTING, METHODS DNA methylation patterns from WBC and CC were assessed for infertile patients undergoing ovarian stimulation at a university-affiliated private practice. DNA was isolated from peripheral blood samples and CC. Bisulfite conversion was then performed and a DNA methylation array was utilized to measure DNA methylation levels throughout the genome. Likelihood ratio tests were utilized to assess the relationship between predicted age, chronologic age and ovarian response. MAIN RESULTS AND THE ROLE OF CHANCE The Horvath-predicted age for WBC samples was consistent with patients’ chronologic age. However, predicted age from analysis of CC was younger than chronologic age. In subgroup analysis of women less than 38 years of age, poor ovarian response was associated with an accelerated predicted age in WBC (P = 0.017). Poor ovarian response did not affect the Horvath-predicted age based on CC samples (P = 0.502). No alternative methylation-based calculation was identified to be predictive of age for CC. LIMITATIONS, REASONS FOR CAUTION To date, analyses of CC have failed to identify epigenetic changes that are predictive of the aging process within the ovary. Despite the poor predictive nature of both the Horvath model and the novel methylation-based age prediction model described here, it is possible that our efforts failed to identify appropriate sites which would result in a successful age-prediction model derived from the CC epigenome. Additionally, lower DNA input for CC samples compared to WBC samples was a methodological limitation. We acknowledge that a universally accepted definition of poor ovarian response is lacking. Furthermore, women with PCOS were included and therefore the group of good responders in the current study may not represent a population with entirely normal methylation profiles. WIDER IMPLICATIONS OF THE FINDINGS The process of ovarian and CC aging continues to be poorly understood. Women who demonstrate poor ovarian response to stimulation represent a common clinical challenge, so clarifying the exact biological changes that occur within the ovary over time is a worthwhile endeavor. The data from CC support a view that hormonally responsive tissues may possess distinct epigenetic aging patterns when compared with other tissue types. Future studies may be able to determine whether alternative DNA methylation sites can accurately predict chronologic age or ovarian response to stimulation from CC samples. Going forward, associations between epigenetic age acceleration and reproductive and general health consequences must also be clearly defined. STUDY FUNDING/COMPETING INTEREST(S) No external funding was obtained for the study and there are no conflicts of interest. TRIAL REGISTRATION NUMBER N/A.

Objectives The objectives of this study were (1) to assess the prevalence of ultrasound (US) features of adenomyosis in an infertile population undergoing in-vitro fertilization (IVF), (2) to define the inter- and intrarater agreement of three-dimensional (3D) US assessment of adenomyosis, and (3) to evaluate sonographic features of adenomyosis with respect to pregnancy outcome following transfer of a single thawed euploid blastocyst. Methods This was a prospective cohort study. Subjects scheduled to undergo a single thawed euploid blastocyst transfer between April and December 2017 at a large IVF center were eligible for inclusion. Enrolled subjects underwent endometrial preparation for frozen embryo transfer. 3D-US was performed on the day prior to embryo transfer, with images stored for subsequent evaluation. Subjects then underwent transfer of a single thawed euploid blastocyst, and pregnancy outcomes were collected. All 3D-US volumes were de-identified and reviewed independently by five reproductive endocrinologists/infertility specialists with expertise in gynecological US for the presence of seven sonographic features of adenomyosis: global uterine enlargement, myometrial wall asymmetry, heterogeneous echogenicity, irregular junctional zone, myometrial cysts, fan-shaped shadowing and ill-defined myometrial lesions. Adenomyosis was considered to be present if the majority of the reviewers noted at least one of the seven sonographic features. Inter- and intrarater agreement was evaluated using Fleiss's kappa. Clinical and cycle characteristics of subjects with and those without adenomyosis were compared. The primary outcome of interest was live birth rate. Secondary outcomes included clinical pregnancy rate and miscarriage rate. Logistic regression analysis was performed to account for potential confounders. Results A total of 648 subjects were included. The prevalence of adenomyosis on US was 15.3% (99/648). On retrospective chart review, very few patients with adenomyosis had symptoms. The inter- and intrarater agreement amongst five independent specialists conducting the 3D-US assessments of adenomyosis were poor (κ = 0.23) and moderate (κ = 0.58), respectively. Subjects with adenomyosis were older (37.1 vs 35.9 years, P = 0.02) and more likely to undergo a gonadotropin-releasing hormone agonist downregulation protocol when compared with those without adenomyosis (12.1% vs 5.1%, P = 0.02). Clinical pregnancy (80.0% vs 75.0%) and live birth (69.5% vs 66.5%) rates were similar between the groups. When adjusting for potential confounders, there was no difference in the rate of clinical pregnancy (adjusted odds ratio (aOR), 1.47 (95% CI, 0.85-2.56)), miscarriage (aOR, 1.3 (95% CI, 0.62-2.72)) or live birth (aOR, 1.28 (95% CI, 0.78-2.08)) between subjects with and those without adenomyosis. No individual sonographic marker of adenomyosis was predictive of pregnancy outcome. Conclusions The inter-rater agreement of 3D-US assessment of adenomyosis is poor. Furthermore, sonographic markers of adenomyosis in asymptomatic patients may not be associated with altered pregnancy outcome following transfer of a single thawed euploid blastocyst. These findings suggest that routine screening for asymptomatic adenomyosis in an unselected infertile patient population undergoing frozen embryo transfer may not be warranted. © 2020 International Society of Ultrasound in Obstetrics and Gynecology.

Camera trap technology has galvanized the study of predator–prey ecology in wild animal communities by expanding the scale and diversity of predator–prey interactions that can be analysed. While observational data from systematic camera arrays have informed inferences on the spatiotemporal outcomes of predator–prey interactions, the capacity for observational studies to identify mechanistic drivers of species interactions is limited. Experimental study designs that utilize camera traps uniquely allow for testing hypothesized mechanisms that drive predator and prey behaviour, incorporating environmental realism not possible in the laboratory while benefiting from the distinct capacity of camera traps to generate large datasets from multiple species with minimal observer interference. However, such pairings of camera traps with experimental methods remain underutilized. We review recent advances in the experimental application of camera traps to investigate fundamental mechanisms underlying predator–prey ecology and present a conceptual guide for designing experimental camera trap studies. Only 9% of camera trap studies on predator–prey ecology in our review use experimental methods, but the application of experimental approaches is increasing. To illustrate the utility of camera trap-based experiments using a case study, we propose a study design that integrates observational and experimental techniques to test a perennial question in predator–prey ecology: how prey balance foraging and safety, as formalized by the risk allocation hypothesis. We discuss applications of camera trap-based experiments to evaluate the diversity of anthropogenic influences on wildlife communities globally. Finally, we review challenges to conducting experimental camera trap studies. Experimental camera trap studies have already begun to play an important role in understanding the predator–prey ecology of free-living animals, and such methods will become increasingly critical to quantifying drivers of community interactions in a rapidly changing world. We recommend increased application of experimental methods in the study of predator and prey responses to humans, synanthropic and invasive species, and other anthropogenic disturbances.

Harvest records suggest that the abundance of bobcats ( Lynx rufus) has increased and the leading edge of their distribution has spread northward, while the trailing edge of the Canada lynx ( Lynx canadensis) range has contracted in Ontario, Canada. There has been a debate about whether these closely related felids might compete in areas of sympatry, but there is little research on sympatric populations of bobcat and lynx. Both species are found on the north shore of Lake Huron in Ontario, Canada, which provided an opportunity to investigate their spatial patterns and habitat use. We surveyed snowmobile routes for snow tracks over three winters and estimated probability of occupancy for the two felid species while accounting for detectability. Bobcat and lynx tracks were never found on the same survey route. Bobcat occupancy increased with habitat heterogeneity, whereas lynx occupancy increased with homogeneity. Our results fit with the common assumption of the generalist and specialist natures of bobcat and lynx, respectively. Our findings suggest that bobcats invaded former lynx territory after these areas became vacant. The story of the bobcat and the lynx is one of the loss of a unique, boreal specialist due to anthropogenic change, and eventual replacement by an adaptable generalist.

Sperm cryopreservation is a vital tool in amphibian assisted reproductive technologies that aids in genetic and population management, specifically for at-risk species. Significant advancements have been made in the cryopreservation of amphibian sperm, yet there is little information on how the cryopreservation process influences fertilization and embryonic development. In this study, we tested several cryoprotective agents (CPAs) and freezing rates on sperm recovery, fertilization potential and embryo development using Fowler’s toads (Anaxyrus fowleri) as a model amphibian species for application to at-risk anurans. Three cryoprotectant treatments were tested, which included 10% trehalose + 0.25% bovine serum albumin with (1) 5% N,N-dimethylformamide (DMFA); (2) 10% DMFA; or (3) 10% dimethyl sulfoxide (DMSO). Additionally, sperm in each cryoprotectant was frozen at two different rates, −32 to −45°C/min and −20 to −29°C/min. Post-thaw sperm analysis included motility, morphology, viability, fertilization success and embryo development. Results show that 10% DMFA produced significantly higher (P = 0.005) post-thaw sperm motility than 5% DMFA and was similar to 10% DMSO. Furthermore, sperm frozen at −32 to −45°C/min had significantly higher post-thaw motility (P

Observations of surface magnetic fields of cool stars reveal a large diversity of configurations. Although there is now a consensus that these fields are generated through dynamo processes occurring within the convective zone, the physical mechanism driving such a variety of field topologies is still debated. This paper discusses the possible origins of dipole and multipole-dominated morphologies using three-dimensional numerical simulations of stratified systems where the magnetic feedback on the fluid motion is significant. Our main result is that dipolar solutions are found at Rossby numbers up to 0.4 in strongly stratified simulations, where previous works suggested that only multipolar fields should exist. We argue that these simulations are reminiscent of the outlier stars observed at Rossby numbers larger than 0.1, whose large-scale magnetic field is dominated by their axisymmetric poloidal component. As suggested in previous Boussinesq calculations, the relative importance of inertial over Lorentz forces is again controlling the dipolar to multipolar transition. Alternatively, we find that the ratio of kinetic to magnetic energies can equally well capture the transition in the field morphology. We test the ability of this new proxy to predict the magnetic morphology of a few M-dwarf stars whose internal structure matches that of our simulations and for which homogeneous magnetic field characterization is available. Finally, the magnitude of the differential rotation obtained in our simulations is compared to actual measurements reported in the literature for M-dwarfs. In our simulations, we find a clear relationship between anti-solar differential rotation and the emergence of dipolar fields., Comment: 15 pages, 15 figures, accepted for publication in MNRAS

We report the detection and characterization of the transiting sub-Neptune TOI-1759 b, using photometric time-series from TESS and near infrared spectropolarimetric data from SPIRou on the CFHT. TOI-1759 b orbits a moderately active M0V star with an orbital period of $18.849975\pm0.000006$ d, and we measure a planetary radius and mass of $3.06\pm0.22$ R$_\oplus$ and $6.8\pm2.0$ M$_\oplus$. Radial velocities were extracted from the SPIRou spectra using both the CCF and the LBL methods, optimizing the velocity measurements in the near infrared domain. We analyzed the broadband SED of the star and the high-resolution SPIRou spectra to constrain the stellar parameters and thus improve the accuracy of the derived planet parameters. A LSD analysis of the SPIRou Stokes $V$ polarized spectra detects Zeeman signatures in TOI-1759. We model the rotational modulation of the magnetic stellar activity using a GP regression with a quasi-periodic covariance function, and find a rotation period of $35.65^{+0.17}_{-0.15}$ d. We reconstruct the large-scale surface magnetic field of the star using ZDI, which gives a predominantly poloidal field with a mean strength of $18\pm4$ G. Finally, we perform a joint Bayesian MCMC analysis of the TESS photometry and SPIRou RVs to optimally constrain the system parameters. At $0.1176\pm0.0013$ au from the star, the planet receives $6.4$ times the bolometric flux incident on Earth, and its equilibrium temperature is estimated at $433\pm14$ K. TOI-1759 b is a likely gas-dominated sub-Neptune with an expected high rate of photoevaporation. Therefore, it is an interesting target to search for neutral hydrogen escape, which may provide important constraints on the planetary formation mechanisms responsible for the observed sub-Neptune radius desert., Accepted for publication in the 10. Planets and planetary systems section of Astronomy & Astrophysics