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An immobilized derivative of γ-gluamylcysteine synthetase was prepared by chemically combining γ-glutamylcysteine synthetase with Sepharose 4B. The immobilized enzyme had a similar pH-activity profile to that of the native one and was much more stable to heat than the native enzyme. The immobilized enzyme retained its full activity after storage at least for 5 months at 4°C, while the native enzyme lost its activity within 2 weeks under the same conditions.

A modified polystyrene-divinylbenzene copolymer, in which ethylcarbodiimidomethyl groups were linked so as to be available as a condensing agent for peptide synthesis, were prepared. This polymer proved of great use in synthesizing some protected peptides without accompanying any troublesome by-product which would otherwise be formed.