1. Development of a prediction model for short-term remission of patients with Crohn’s disease treated with anti-TNF drugs
- Author
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Rosario Medina-Medina, Eva Iglesias-Flores, Jose M. Benítez, Sandra Marín-Pedrosa, Isabel Salgueiro-Rodríguez, Clara I. Linares, Sandra González-Rubio, Pilar Soto-Escribano, Beatriz Gros, Manuel L. Rodríguez-Perálvarez, José L. Cabriada, María Chaparro, Javier P. Gisbert, Eduardo Chicano-Gálvez, Ignacio Ortea, Gustavo Ferrín, Valle García-Sánchez, and Patricia Aguilar-Melero
- Subjects
Crohn’s disease ,Organic Chemistry ,General Medicine ,Catalysis ,Inflammatory bowel disease ,Anti-TNF-a therapy ,Vinculin ,Computer Science Applications ,Inorganic Chemistry ,SWATH proteomics ,Predictive biomarkers ,Physical and Theoretical Chemistry ,Molecular Biology ,inflammatory bowel disease ,predictive biomarkers ,anti-TNF-a therapy ,vinculin ,Spectroscopy - Abstract
Therapy with anti-tumor necrosis factor (TNF) has dramatically changed the natural history of Crohn’s disease (CD). However, these drugs are not without adverse events, and up to 40% of patients could lose efficacy in the long term. We aimed to identify reliable markers of response to anti-TNF drugs in patients with CD. A consecutive cohort of 113 anti-TNF naive patients with CD was stratified according to clinical response as short-term remission (STR) or non-STR (NSTR) at 12 weeks of treatment. We compared the protein expression profiles of plasma samples in a subset of patients from both groups prior to anti-TNF therapy by SWATH proteomics. We identified 18 differentially expressed proteins (p ≤ 0.01, fold change ≥ 2.4) involved in the organization of the cytoskeleton and cell junction, hemostasis/platelet function, carbohydrate metabolism, and immune response as candidate biomarkers of STR. Among them, vinculin was one of the most deregulated proteins (p < 0.001), whose differential expression was confirmed by ELISA (p = 0.054). In the multivariate analysis, plasma vinculin levels along with basal CD Activity Index, corticosteroids induction, and bowel resection were factors predicting NSTR.
- Published
- 2023