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1. Use of the 13C/12C, 15N/14N and 18O/16O Isotopic Ratios of Theobromine and Caffeine in the Characterization of Geographic Origin

Author

Didier Diomande, Maël Leroux, Illa Tea, Jacques Sagne Akpa, Adjon Kouassi, Gérald S. Remaud, and Ingrid Antheaume

Subjects
Psychiatry and Mental health, chemistry.chemical_compound, δ13C, chemistry, δ18O, Geographic origin, Principal component analysis, medicine, Food science, Biology, Caffeine, Theobromine, medicine.drug
Abstract

The aim of this work is to characterize the geographical origin of cocoa beans and coffee beans. This study aims to contribute to the traceability of raw materials in order to fight against falsification. For this purpose, we based our work on the measurement of isotope ratios in 13C, 15N and 18O. The multi-element isotope ratios have been evaluated as a means to distinguish fermented cocoa beans of different geographic and varietal origins. The isotopic ratios of 13C, 15N and 18O were measured in theobromine obtained from samples of fermented cocoa beans. Twenty-two (22) samples of different geographical origins covering the four mainland cocoa producing areas were analyzed on the one hand and on the other hand, 16 caffeine samples from various origins were also analyzed. The treatment of the values resulting from these isotopic analyzes by statistical methods, namely the principal component analysis (PCA) makes it possible to visualize the discriminations between the different origins. The most discriminating variables identified as responsible for the geographic and varietal differences were the δ15N, δ13C and δ18O values of cocoa beans and certain extracts and tissues. We have shown that the isotope ratios are correlated with the altitude and precipitation conditions encountered in the different cocoa growing regions.

Published
2021
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2. Influence of aphotic haloclines and euxinia on organic biomarkers and microbial communities in a thalassohaline and alkaline volcanic crater lake

Author

Mylène Hugoni, Cécile Bernard, Ingrid Antheaume, Gérard Sarazin, Hélène Agogué, Christophe Leboulanger, Vincent Grossi, Didier Jézéquel, David Sala, Magali Ader, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de Physique du Globe de Paris (IPGP (UMR_7154)), Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Microbiologie, adaptation et pathogénie (MAP), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Institut de Physique du Globe de Paris (IPGP), and Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)

Subjects
Biogeochemical cycle, haline and aphotic euxinia, WSA2 methanogenic 69 Euryarchaeota, Thermoplasmata, Haline and aphotic euxinia, Bacteroidetes ML635J-40 aquatic group, syntrophomonadaceae, 03 medical and health sciences, Water column, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, RNA, Ribosomal, 16S, Crater lake, Organic matter, Photic zone, 14. Life underwater, lipid biomarkers, WSA2 methanogenic Euryarchaeota, Phylogeny, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, General Environmental Science, chemistry.chemical_classification, 0303 health sciences, biology, 030306 microbiology, Microbiota, bacterial and archaeal diversity, 15. Life on land, bacteroidetes ML635J-40 aquatic group, biology.organism_classification, Archaea, Anoxic waters, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 6. Clean water, Lakes, chemistry, 13. Climate action, Aphotic zone, Environmental chemistry, General Earth and Planetary Sciences, Syntrophomonadaceae, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Biomarkers
Abstract

International audience; Studies on microbial communities, and their associated organic biomarkers, that are found thriving in the aphotic euxinic waters in modern stratified ecosystems are scarce compared to those undertaken in euxinic photic zones. The Dziani Dzaha (Mayotte,Indian Ocean) is a tropical, saline, alkaline crater lake that has recently been presentedas a modern analog of Proterozoic Oceans due to its thalassohaline classification (having water of marine origin) and specific biogeochemical characteristics. Continuous intense photosynthetic production and microbial mineralization keep most of the water column permanently aphotic and anoxic preventing the development of a euxinic (sulfidic and anoxic) photic zone despite a high sulfide/sulfate ratio and the presence of permanent or seasonal haloclines. In this study, the molecular composition of the organic matter in Lake Dziani Dzaha was investigated and compared to the microbial diversity evaluated through 16S rRNA gene amplicon sequencing, over two contrasting seasons (rainy vs. dry) that influence water column stratification. Depth profiles of organic biomarker concentrations (chlorophyll-a and lipid biomarkers) and bacterial and archaeal OTU abundances appeared to be strongly dependent on the presence of aphotic haloclines and euxinia. OTU abundances revealed theimportance of specific haloalkaliphilic bacterial and archaeal assemblages in phytoplanktonic biomass recycling and the biogeochemical functioning of the lake, suggesting new haloalkaline non-phototrophic anaerobic microbial precursors for some of the lipid biomarkers. Uncultured Firmicutes from the family Syntrophomonadaceae (Clostridiales), and Bacteroidetes from the ML635J-40 aquatic group, emerged as abundant chemotrophic bacterial members in the anoxic or euxinic waters and were probably responsible for the production of short-chain n-alkenes, wax esters, diplopterol, and tetrahymanol. Halocline-dependent euxinia also had a strong impact on the archaeal community which was dominated by Woesearchaeota in the sulfide-free waters. In the euxinic waters, methanogenic Euryarchaeota from the Methanomicrobia, Thermoplasmata, and WSA2 classes dominated and were likely at the origin of common hydrocarbon biomarkers of methanogens (phytane, pentamethyl-eicosenes, and partially hydrogenated squalene).

Published
2022
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3. LATE HOLOCENE MONGOLIAN CLIMATE RECONSTRUCTIONS FROM LOCALLY CALIBRATED GDGT AND POLLEN TRANSFER FUNCTIONS FOR LAKE AYRAG

Author

Matthew Makou, Guillemette Ménot, Bazartseren Boldgiv, Sébastien Joannin, Lucas Dugerdil, Salomé Ansanay-Alex, Anne-Lise Develle, Vincent Grossi, Jérôme Magail, Hermann Behling, Julia Unkelbach, Odile Peyron, Isabelle Jouffroy-Bapicot, Ingrid Antheaume, Boris Vannière, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Université de Montpellier (UM), École normale supérieure - Lyon (ENS Lyon), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Environnements, Dynamiques et Territoires de la Montagne (EDYTEM), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), National University of Mongolia, University of Göttingen - Georg-August-Universität Göttingen, Musée d'anthropologie préhistorique de Monaco, Principauté de Monaco, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chrono-environnement (UMR 6249) (LCE), Environnements, Dynamiques et Territoires de Montagne (EDYTEM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Georg-August-University = Georg-August-Universität Göttingen, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Massachusetts General Hospital [Boston], Palynology and Climate Dynamics, Albrecht-von-Haller-Inst. for Plant Sciences, and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)

Subjects
[SDE.MCG]Environmental Sciences/Global Changes, [SDV]Life Sciences [q-bio], medicine.disease_cause, [SHS]Humanities and Social Sciences, Sequence (geology), 13. Climate action, Pollen, [SDE]Environmental Sciences, medicine, Physical geography, [SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces, environment, Geology, Holocene, ComputingMilieux_MISCELLANEOUS
Abstract

Summary The 4,000 year cal. BP pollen and brGDGT sequence from Lake Ayrag documents the Mongolian Late Holocene climate and human impact variations.

Published
2021
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4. The role of early diagenesis in the shaping of geochemical records : an example from Lake Dziani Dzaha, Mayotte

Author

Emmanuel Malet, François Baudin, Vincent Grossi, Pierre Cartigny, Ingrid Antheaume, Francois Gelin, Laurent Simon, Manuela Capano, Salomé Mignard, Magali Ader, Fabien Arnaud, Edouard Bard, Pierre Adam, Ivan Jovovic, Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique du Globe de Paris (IPGP (UMR_7154)), Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut des Sciences de la Terre de Paris (iSTeP), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Environnements, Dynamiques et Territoires de Montagne (EDYTEM), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Centre scientifique et Technique Jean Feger (CSTJF), and TOTAL FINA ELF

Subjects
web-evolution-climat-ocean, 2HAL, [SDU.STU.CL]Sciences of the Universe [physics]/Earth Sciences/Climatology, Geochemistry, traité, Chaires, BARD Édouard, Geology, Document sous DOI (Digital Object Identifier), Diagenesis, Texte intégral (accès ouvert)
Abstract

https://hal.archives-ouvertes.fr/hal-03252301; International audience

Published
2021
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5. Serpentinization in formate-bearing fluids: an experimental approach at 300 °C-25 MPa

Author

Muriel Andreani, Hervé Cardon, Ingrid Antheaume, Xavier Saupin, Patrick Jame, Emmanuelle Albalat, Olivier Sissmann, Erik Bonjour, Samuel Barbier, Eric C. Gaucher, Vincent Grossi, Clementine Fellah, Bénédicte Ménez, Isabelle Daniel, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Centre scientifique et Technique Jean Feger (CSTJF), TOTAL FINA ELF, IFP Energies nouvelles (IFPEN), Institut de Physique du Globe de Paris (IPGP (UMR_7154)), Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Isotopique & Organique - Isotopic & Organic, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), the European Association of Geochemistry and the Geochemical Society, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Total SA - CSTJF, Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
chemistry.chemical_compound, Bearing (mechanical), Chemistry, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Formate, ComputingMilieux_MISCELLANEOUS, Nuclear chemistry, law.invention
Abstract

International audience

Published
2021

7. brGDGTs and isotopic records for Lake Ayrag: implications for climate, vegetation and human impact history of the Mongolian Plateau

Author

Julia Unkelbach, Matthew Makou, Sébastien Joannin, Guillemette Ménot, Boris Vannière, Bazartseren Boldgiv, Lucas Dugerdil, Hermann Behling, Anne-Lise Develle, Salomé Ansanay-Alex, Odile Peyron, Mary Robles, Vincent Grossi, Jérôme Magail, Ingrid Antheaume, and Isabelle Jouffroy-Bapicot

Subjects
geography, Plateau, geography.geographical_feature_category, medicine, Physical geography, medicine.symptom, Vegetation (pathology), Geology
Published
2021
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8. Plant Wax n ‐Alkane and n ‐Alkanoic Acid Signatures Overprinted by Microbial Contributions and Old Carbon in Meromictic Lake Sediments

Author

Ingrid Antheaume, Vincent Grossi, Daniel B. Montluçon, Matthew Makou, Timothy I. Eglinton, Cameron McIntyre, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, and Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)

Subjects
010504 meteorology & atmospheric sciences, meromictic lake, chemistry.chemical_element, 010502 geochemistry & geophysics, 01 natural sciences, law.invention, n-alkane, compound specific radiocarbon analysis, law, plant wax, [CHIM]Chemical Sciences, Radiocarbon dating, Alkanoic acid, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Alkane, chemistry.chemical_classification, Wax, Hard water, Geophysics, Deposition (aerosol physics), chemistry, [SDU]Sciences of the Universe [physics], 13. Climate action, Environmental chemistry, visual_art, [SDE]Environmental Sciences, Soil water, n-alkanoic acid, visual_art.visual_art_medium, biomarker, General Earth and Planetary Sciences, Carbon
Abstract

International audience; Specific n-alkanes and n-alkanoic acids are commonly used as biomarkers in paleoenvironmental reconstruction, yet any individual homologue may originate from multiple biological sources. Here we improve source and age controls for these compounds in meromictic systems by measuring the radiocarbon (C-14) ages of specific homologues preserved in twentieth century Lake Pavin (France) sediments. In contrast to many studies, C-14 ages generally decreased with increasing carbon chain length, from 7.3 to 2.6 ka for the C-14-C-30 n-alkanoic acids and from 9.2 to 0.3 ka for the C-21-C-33 n-alkanes. Given a known hard water effect, these values suggest that aquatic microbial sources predominate and contributed to most of the homologues measured. Only the longest chain n-alkanes exclusively represent inputs of higher plant waxes, which were previously sequestered in soils over centennial to millennial timescales prior to transport and deposition. These findings suggest that biomarker source and age should be carefully established for lacustrine settings.

Published
2018
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9. Quantitative and specific recovery of natural organic and mineral sulfur for (multi-)isotope analysis

Author

Pierre Cartigny, Laurent Simon, Ingrid Antheaume, V. Grossi, Pierre Adam, F. Gelin, Magali Ader, I. Jovovic, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), Centre scientifique et Technique Jean Feger (CSTJF), TOTAL FINA ELF, Institut de Physique du Globe de Paris (IPGP (UMR_7154)), Institut national des sciences de l'Univers (INSU - CNRS)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut de Physique du Globe de Paris (IPGP), and Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)

Subjects
010504 meteorology & atmospheric sciences, chemistry.chemical_element, Source rocks, 010502 geochemistry & geophysics, Mass spectrometry, 01 natural sciences, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry, Geochemistry and Petrology, Kerogen, Wet oxidation, Inductively coupled plasma mass spectrometry, Organic and mineral sulfur, Chemical oxidation of organic sulfur, 0105 earth and related environmental sciences, Isotope analysis, S biogeochemical cycle, Chemistry, Stable isotope ratio, Sulfur, Sulfur (multi-)isotopes, 13. Climate action, Environmental chemistry, [SDE]Environmental Sciences, Wet chemistry
Abstract

International audience; Deciphering the role of sulfur in biogeochemical cycles strongly relies on its stable isotope composition, which ultimately depends on the ability to quantitatively recover different sulfur species from geological samples. For decades most studies have been restricted to the 34S/32S composition of bulk samples, using simple methods based on the analysis of SO2 released by sample combustion combined to mass spectrometry. The wet chemistry procedures required to selectively extract specific sulfur species were generally avoided due to their tediousness and inefficiency for some complex matrices, especially when targeting organic sulfur. With the recent advent of multi-isotope studies (investigating the minor sulfur stable isotopes 33S and 36S) which rely either on the analysis of sulfur as SF6, or on the use of secondary ion or multi-collector inductively coupled plasma mass spectrometry, wet chemistry-based preparation procedures were brought back to the stage with a renewed interest in developing procedures better adapted to the investigation of specific sulfur species. Here we propose a new stepwise chemical procedure for the quantitative recovery and multi-isotope analysis of organic sulfur from both solvent soluble (total lipid extract) and insoluble (kerogen) fractions, based on a wet oxidation by sodium hypochlorite. This procedure also allows the multi-isotope analysis of inorganic sulfur species (elemental sulfur, sulfates and sulfides) in the same sample. Its application to different well-known petroleum source rocks and to an oil demonstrates its relevance for disentangling the interactions between the different sulfur pools and for shedding new light on the sulfur biogeochemical cycle.

Published
2020
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10. A New Method for Organic and Mineral Sulfur Multi-Isotope Analysis of Sediments and Petroleum Source Rocks

Author

Ingrid Antheaume, F. Gelin, Pierre Cartigny, Pierre Adam, Vincent Grossi, Magali Ader, I. Jovovic, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Total, Total E&P, Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Mineral, Source rock, chemistry, [SDE]Environmental Sciences, Geochemistry, [CHIM]Chemical Sciences, chemistry.chemical_element, Sulfur, ComputingMilieux_MISCELLANEOUS, Geology, Isotope analysis
Abstract

International audience

Published
2019
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11. Coupled abiotic and biotic sulfurization processes during microbial sulfate reduction with alkyl substrates

Author

Vincent Grossi, Ingrid Antheaume, Cristiana Cravo-Laureau, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Abiotic component, chemistry.chemical_classification, 0209 industrial biotechnology, 010401 analytical chemistry, 02 engineering and technology, 01 natural sciences, 0104 chemical sciences, Reduction (complexity), chemistry.chemical_compound, 020901 industrial engineering & automation, chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Environmental chemistry, [SDE]Environmental Sciences, Sulfate, Alkyl, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

12. Recycling of archaeal biomass as a new strategy for extreme life in Dead Sea deep sediments

Author

Daniel Ariztegui, Camille Thomas, Ingrid Antheaume, Vincent Grossi, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Forel and Department of Geology and Paleontology, University of Geneva [Switzerland], Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Université de Genève = University of Geneva (UNIGE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)

Subjects
bepress|Physical Sciences and Mathematics, EarthArXiv|Physical Sciences and Mathematics|Chemistry|Organic Chemistry, 010504 meteorology & atmospheric sciences, Earth science, Heterotroph, Biomass, bepress|Physical Sciences and Mathematics|Earth Sciences, engineering.material, EarthArXiv|Physical Sciences and Mathematics|Earth Sciences, 010502 geochemistry & geophysics, 01 natural sciences, Extremophiles, ddc:550, Extreme environment, [CHIM]Chemical Sciences, EarthArXiv|Physical Sciences and Mathematics|Chemistry, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Total organic carbon, bepress|Physical Sciences and Mathematics|Chemistry, biology, Biosphere, Geology, biology.organism_classification, Geomicrobiology, EarthArXiv|Physical Sciences and Mathematics, Dead Sea, EarthArXiv|Physical Sciences and Mathematics|Earth Sciences|Biogeochemistry, bepress|Physical Sciences and Mathematics|Chemistry|Organic Chemistry, [SDU]Sciences of the Universe [physics], 13. Climate action, [SDE]Environmental Sciences, engineering, Halite, Sedimentary rock, bepress|Physical Sciences and Mathematics|Earth Sciences|Biogeochemistry, Archaea
Abstract

Archaea and Bacteria that inhabit the deep subsurface (known as the deep biosphere) play a prevalent role in the recycling of sedimentary organic carbon. In such environments, this process can occur over millions of years and requires microbial communities to cope with extremely limited sources of energy. Because of this scarcity, metabolic processes come at a high energetic cost, but the ways heterotrophic microbial communities develop to minimize energy expenses for a maximized yield remain unclear. Here, we report molecular biomarker evidence for the recycling of archaeal cell wall constituents in extreme evaporitic facies of Dead Sea deep sediments. Wax esters derived from the recombination of hydrolyzed products of archaeal membrane lipids were observed in gypsum and/or halite sedimentary deposits down to 243 m below the lake floor, implying the reutilization of archaeal necromass possibly by deep subsurface bacteria. By recycling the building blocks of putatively better-adapted archaea, heterotrophic bacteria may build up intracellular carbon stocks and mitigate osmotic stress in this energy-deprived environment. This mechanism illustrates a new pathway of carbon transformation in the subsurface and demonstrates how life can be maintained in extreme environments characterized by long-term isolation and minimal energetic resources.

Published
2017
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14. 13 C and 15 N natural isotope abundance reflects breast cancer cell metabolism

Author

Caroline Mauve, Sophie Barillé-Nion, Françoise Gilard, Ingrid Antheaume, Julie Lalande, Anneke C. Blackburn, Estelle Martineau, Guillaume Tcherkez, Illa Tea, Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Cancer Metabolism and Genetics Group [Canberra, Australia], Australian National University (ANU)-The John Curtin School of Medical Research, Cellule de compétences SPECTROMAITRISE, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Laennec-Centre National de la Recherche Scientifique (CNRS)-Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes (CHU Nantes), Research School of Biology [Canberra, Australia], Australian National University (ANU), Bernardo, Elizabeth, Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST)

Subjects
0301 basic medicine, Multidisciplinary, medicine.diagnostic_test, Receptor expression, Metabolite, Cancer, Lipid metabolism, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, medicine.disease, Article, 3. Good health, Biomarker (cell), 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Metabolomics, Breast cancer, Biochemistry, chemistry, [SDV.CAN] Life Sciences [q-bio]/Cancer, Biopsy, Cancer research, medicine
Abstract

International audience; Breast cancer is the most common cancer in women worldwide. Despite the information provided by anatomopathological assessment and molecular markers (such as receptor expression ER, PR, HER2), breast cancer therapies and prognostics depend on the metabolic properties of tumor cells. However, metabolomics have not provided a robust and congruent biomarker yet, likely because individual metabolite contents are insufficient to encapsulate all of the alterations in metabolic fluxes. Here, we took advantage of natural 13 C and 15 N isotope abundance to show there are isotopic differences between healthy and cancer biopsy tissues or between healthy and malignant cultured cell lines. Isotope mass balance further suggests that these differences are mostly related to lipid metabolism, anaplerosis and urea cycle, three pathways known to be impacted in malignant cells. Our results demonstrate that the isotope signature is a good descriptor of metabolism since it integrates modifications in C partitioning and N excretion altogether. Our present study is thus a starting point to possible clinical applications such as patient screening and biopsy characterization in every cancer that is associated with metabolic changes. Medical applications of stable isotopes are now widespread, like the well-known 13 C-urea breath assay for ulcer detection 1. This takes advantage of 13 C-labelling and thus usually neglects differences in reaction rates between isotopic forms, because the isotopic signal used for diagnosing is far above small natural variations in 13 C. By contrast, the use of isotopes at natural abundance exploits such subtle differences (referred to as isotope effects) to identify bottlenecks in metabolic pathways (rate-limiting steps) or the contribution of multiple elemental sources (mass balance), without the need to introduce expensive isotope tracers into the patient. Isotope effects in metabolism are mostly caused by enzymatic reactions that preferentially consume substrates containing either the light or the heavy isotope (isotopologues) and therefore, the natural isotope abundance in metabolites depends on metabolic fluxes and source substrates 2. For example, the natural 13 C abundance in respired CO 2 has been used to trace diet composition and substrate changes during exercise 3,4. In cancer biology, the use of natural variations in Cu and S stable isotopes in hepatocellular carcinoma has been attempted recently 5. But to our knowledge, no study has looked at alterations of natural isotope abundance in breast cancer. Due to changes in primary C and N metabolism such as increased glycolysis, glutaminolysis and nucleotide synthesis 6 , important changes in 13 C and 15 N natural abundance can be anticipated. To address this question, we examined the isotopic signature of intact breast cancer biopsies (mostly from invasive ductal carcinoma, IDC) and cultured breast cancer cell lines (Supplementary Tables S1 and S2) using elemental analysis coupled to isotope ratio mass spectrometry (EA-IRMS). This technique has been recently shown to be applicable to the biochemical analysis of cancerous cell lines 7 .

Published
2016
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15. Determination of the concentration of nitrogenous bio-organic compounds using an isotope ratio mass spectrometer operating in continuous flow mode

Author

Richard J. Robins, Ingrid Antheaume, Illa Tea, Katarzyna Kosieradzka, and Emmanuel Gentil

Subjects
Detection limit, Analyte, Nitrogen Isotopes, Isotope, Taurine, Chemistry, Analytical chemistry, Mass spectrometry, Glutathione, Biochemistry, Chemistry Techniques, Analytical, Mass Spectrometry, Isotopes of nitrogen, Analytical Chemistry, Ion, law.invention, Alkaloids, Limit of Detection, law, Pseudomonas, Flame ionization detector, Cysteine, Isotope-ratio mass spectrometry, Tropanes
Abstract

The quality of the determination of compound-specific isotopic content at natural abundance by gas chromatography-isotope ratio measurement-mass spectrometry (GC-irm-MS) relies on the stability of the voltage generated by the ion detector Faraday cages. The application of GC-irm-MS to the determination of δ(13)C (‰) and δ(15)N (‰) is now routine. However, for numerous applications, it is necessary to determine both the isotope content (δ(15)N) and the quantity (in micromoles) of analyte present. We now show that it is possible for nitrogen-containing compounds to measure how much analyte is present with an irm mass spectrometer linked to a GC by exploiting the integrated N(2) total ion current intensity (Vs) generated by measuring the (15)N/(14)N isotope ratio. The method is validated over a range of concentration (2-70 mmol/L) and δ(15)N (-70 to +50‰) values for six molecules of diverse chemical nature and functionality (nortropine, norpseudotropine, nortropinone, cysteine, taurine, glutathione). It is shown that once the ion current is calibrated, the quantitative values are of a comparable quality to those obtained from GC with flame ionization detection (GC-FID). In addition, it is demonstrated that over a definable range, the δ(15)N (‰) value is independent of the quantity of analyte introduced, confirming the validity of this method.

Published
2011
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16. Human baby hair amino acid natural abundance 15N-isotope values are not related to the 15N-isotope values of amino acids in mother’s breast milk protein

Author

Richard J. Robins, Katarzyna M. Romek, Maxime Julien, Illa Tea, Régis Hankard, Ingrid Antheaume, M. Frasquet-Darrieux, Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Centre hospitalier universitaire de Poitiers (CHU Poitiers)

Subjects
Adult, 0301 basic medicine, Clinical Biochemistry, 15n isotope, Breast milk, 01 natural sciences, Biochemistry, 03 medical and health sciences, Isotope fractionation, Abundance (ecology), Humans, [CHIM]Chemical Sciences, 0601 history and archaeology, Food science, Amino Acids, 2. Zero hunger, chemistry.chemical_classification, 060101 anthropology, Milk, Human, Nitrogen Isotopes, Milk protein, δ13C, Chemistry, 010401 analytical chemistry, Organic Chemistry, Infant, 06 humanities and the arts, Milk Proteins, 0104 chemical sciences, Amino acid, 030104 developmental biology, Female, Hair
Abstract

International audience; In the article (Romek et al. 2013) we reported the values of δ15N (‰) and δ13C (‰) obtained bySince exclusively breast-suckled infants obtain their nutrient only from their mother's milk, it might be anticipated that a correlation will exist between the (15)N/(14)N isotope ratios of amino acids of protein of young infants and those supplied by their mother. The work presented here aimed to determine whether amino nitrogen transfer from human milk to infant hair protein synthesized within the first month of life conserves the maternal isotopic signature or whether post-ingestion fractionation dominates the nitrogen isotope spectrum. The study was conducted at 1 month post-birth on 100 mother-infant pairs. Isotope ratios (15)N/(14)N and (13)C/(12)C were measured using isotope ratio measurement by Mass Spectrometry (irm-MS) for whole maternal milk, and infant hair and (15)N/(14)N ratios were also measured by GC-irm-MS for the N-pivaloyl-O-isopropyl esters of amino acids obtained from the hydrolysis of milk and hair proteins. The δ(15)N and δ(13)C (‰) were found to be significantly higher in infant hair than in breast milk (δ(15)N, P < 0.001; δ(13)C, P < 0.001). Furthermore, the δ(15)N (‰) of individual amino acids in infant hair was also significantly higher than that in maternal milk (P < 0.001). By calculation, the observed shift in isotope ratio was shown not to be accounted for by the amino acid composition of hair and milk proteins, indicating that it is not simply due to differences in the composition in the proteins present. Rather, it would appear that each pool-mother and infant-turns over independently, and that fractionation in infant N-metabolism even in the first month of life dominates over the nutrient N-content.

Published
2013
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17. A test to identify cyanide origin by isotope ratio mass spectrometry for forensic investigation

Author

Ingrid Antheaume, Illa Tea, and Ben-Li Zhang

Subjects
Orange juice, Chromatography, Cyanide, Carbon-13, Poison control, Pathology and Forensic Medicine, chemistry.chemical_compound, Isotopic signature, chemistry, Elemental analysis, Isotope-ratio mass spectrometry, Law, Nuclear chemistry, Isotope analysis
Abstract

Cyanide is one of the common poisons in murders. When cyanide has been used, to identify the origin of cyanide may be necessary in the forensic investigation. We have examined the possibility of distinguishing different commercial cyanide samples through the δ 13 C and δ 15 N values and developed a protocol for the isotope analysis of cyanide extracted from several matrices as food and medicine. Several cyanide precipitates were tested for the isotope analysis. The results show that cupric ferrocyanide Cu 2 [Fe(CN) 6 ] is the most appropriate precipitate for the analysis. Thirteen batches of KCN and nine batches of NaCN chemicals were randomly chosen from different suppliers. The cyanides were converted to cupric ferrocyanide and then analysed by isotope ratio mass spectrometry coupled to elemental analysis (EA-IRMS). The isotopic signature of the commercial samples varied from −51.96 to −25.77‰ for δ 13 C and from −4.51 to +3.81‰ for δ 15 N, highlighting the potential of applying EA-IRMS technique to identify cyanide from different batches and sources. The influence of the cyanide extraction and isolation from spiked matrix on the isotopic analysis was also studied. Three matrices: orange juice, yogurt drink and a medicine were tested. In many cases, the isotopic analysis results obtained from the original cyanides precipitates and those isolated from the matrices showed a good accordance, especially for δ 15 N. In some matrices, the 13 C analysis was interfered by co-precipitates. With carefully elaborated working protocol, determining the isotope ratio of N and C in cyanide by EA-IRMS is a promising method for forensic investigations.

Published
2011

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