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The article describes cases of saprolegniosis in Pterophyllum scalare in private aquaristics and assesses the therapeutic efficacy of acriflafin chloride against Saprolegnia parasitica infection. To establish the diagnosis, the clinical signs present in sick fish, the results of mycological and microscopic examinations are taken into account. Some chemical and mycological indices of aquarium water have been studied, and also mycological studies of fish feed have been carried out. It is established that the disease of fish develops against the background of adverse changes in physical, chemical composition and microbiocenosis of aquarium water. Low water temperature, high levels of phosphates and pH, a significant level of organic pollution, compared to the norm, provoke the accumulation of opportunistic microbiota, resulting in imbalance in the parasite-host system and the development of clinical manifestations of saprolegniosis in fish. It was found that 44.4% of the studied feed samples fed to fish were contaminated with epiphytic micromycetes. Micromycetes are represented by the genera Aspergillus, Penicilium, Fusarium, Mucor, Rhizopus. Among the studied feeds, the most affected by fungi were larvae of Chironomus plumosus and dry Daphnia pulex. According to the results of our studies during outbreaks of saprolegniosis, the pH of aquarium water was 8.1 ± 0.7, the content of phosphates – 5.6 ± 1.1 mg/L, micromycetes – 18.0 ± 1.2 CFU/100 cm3. Aspergillus flavus, A. niger and Penicillium canescens were detected in the studied water samples. With saprolegniosis, the angelfish have a reduced appetite, spots, ulcers, white thin threads, and a cotton-like plaque appear on certain areas of the skin, fins, eyes, and gills. It is established that effective means for the treatment of sick fish are external use in the form of a long bath of acriflavine chloride and extract of Melaleuca alternifolia. It is also effective to increase the water temperature to 25–27 °С, to ensure the normative fish-holding density in aquariums and to exclude from the diet fish feed contaminated with micromycetes. After using the drugs for two weeks every other day, water was replaced by 20% of the aquarium volume and aerated. As a result of the treatment, gradual healing of skin lesions and recovery of 65% of fish with signs of lesions of the outer coverings were registered. Thus, the article analyzes the causes of saprolegniosis in angelfish common in private aquariums, describes the clinical signs of the disease and assesses the therapeutic efficacy of acriflavine chloride and Melaleuca alternifolia extract against Saprolegnia parasitica infection. Prospects for further research lie in search of more effective and environmentally friendly means for the treatment of saprolegniosis in aquarium fish.

The article presents epizootological data and clinical signs of feline chlamydia in Poltava. According to the results of studies in 2018–2020, a wide spread of chlamydia infection among cats in the city of Poltava was established. The largest number of the studied cats, were pedigree or crossbred. There was no explicit clear seasonality, but a greater number of cases of respiratory diseases were recorded in the autumn-winter period of the year. In the structure of infectious diseases during the research period, chlamydia mono-infection was registered in 68 % of cats, association of chlamydia with infectious rhinotracheitis in 18.5 %, kalitsivirus infection in 8.6 %, with panleukopenia in 4.9 %. The greatest number of the registered cats had acute and subacute course of chlamydia. In the acute course, there was an increase in temperature, oppression and refusal to eat. In the majority of cases of acute, subacute and associated chlamydia, the structures of the eye were damaged. Conjunctivitis, keratitis, chemosis, uveitis, third eyelid inflammation, changes in the shape of the pupil developed. A Sphynx breed cat showed signs of acute chlamydial arthritis with pronounced inflammatory edema. The latent course was accompanied by abortions, which were mainly registered in young cats. Animals older than four years more often showed stillborn and weak kittens. The main clinical signs of chlamydia in kittens born from sick cats were eye lesions: swelling of the conjunctiva, its reddening and secretion of a significant amount of brown purulent exudate with the viscosity of the catarrh and as a consequence of eyelid adhesion and complications as ankyloblepharon or symblepharon. Submandibular and other lymph nodes in the head area were significantly enlarged. Often pus dried up near the mouth or nostrils. Pus crusts accumulated in the nasal cavity and prevented breathing. Gastrointestinal tract disorders were observed in some cases. Part of the affected litter died within the first three days after birth. Neonatal chlamydia was more often manifested by conjunctivitis, keratitis, panophthalmitis and growth retardation. On the second or third day after birth, the kittens showed weakness and lack of appetite. In two cases of neonatal chlamydia, bronchopneumonia developed. When chlamydia and infectious rhinotracheitis were associated, the structures of the eye were affected in all cases. These lesions could be different and depended on the stage of the infectious process.

For suspicion of an infectious disease of cats, diagnostic tests were conducted. From the purulent leakages of the nasal cavity of the cat, pure cultures of the Bordetal, Escherichia and Staphylococcus cultures were obtained. Differential environments were used for this purpose. For effective treatment, each of the isolated cultures was tested for antibacterial sensitivity. According to the results, isolated cultures showed the highest sensitivity to gentamicin and enrofloxacin. Given the sensitivity of the isolated microflora, enrofloxacin-50 was used in the range of 0.1 ml per 1 kg of body weight for 5 days subcutaneously. Cats are a kind of animals that are sensitive to antibacterial substances. The choice of enrofloxacin was due to the lack of reactions and sensitivity to this drug in cats. As the immunomodulatory agents, which increase the overall resistance of the body used Katozal and Gamavat. Katozal was used for cats once a day subcutaneously for 5 days, at a dose of 0.5 ml for 5 days. Gamavit was administered subcutaneously at a dose of 0.3–0.5 ml/kg body weight (therapeutic dose). Tetravit was used as a vitamin preparation. It was injected subcutaneously 0.3 ml; once in 7 days. Improvement of the clinical condition of the diseased animal was observed the second day after the start of treatment; the animal revived, appetite appeared, the mucus-purulent discharge from the nasal cavity stopped. As bordeliosis is transmitted by airborne droplet, air disinfection was carried out using a bactericidal irradiator BactoSfera OBB 36 P with a quartz lamp. To disinfect the bactericidal lamp was switched on every day – in the morning and evening for 20 minutes.

In the article epizootological data, clinical signs, pathological changes and laboratory research associate for the manifestation of pasteurellosis, stafilokokos and viral stomatitis of rabbits. The work was performed in 2016 in the private sector Poltava region. Diagnostics performed comprehensively, taking into account symptoms and results of microscopic and bacteriological research.The disease is filed within three rabbits. Animals lost weight, felt very thirsty, became depressed, inactive. Appetite is usually in early disease did not change, but with the development of clinical signs deteriorated. Developed rhinitis, first serous then mucopurulent. In disorders of respiratory function observed dysfunction of the gastrointestinal tract - diarrhea alternating with constipation, the abdomen becomes soft, not firm. The main clinical feature of this period was a progressive dehydration: dry and anemichnist visible mucous membranes, eyes retraction deep into orbit. The back of the body was contaminated liquid stool consistency. Fur becomes darker, hair becomes disheveled, easily removed from hair follicles. In the corners of the eyes accumulated significant amount of muco-purulent fluid, which in some cases led to a adhesive forever. Rabbits died on the background of progressive weakness.Autopsy of the dead showed signs of sero-hemorrhagic or sero-fibrinous pneumonia, sero-hemorrhagic pleurisy and peritonitis. Recorded splenit in bowel - wall thickening, signs of acute catarrhal enteritis, multiple hemorrhages in the mucosa and signs of venous congestion.In developing control measures take into account the existence of the association. In this economy together with the associated pasteurellosis was diagnosed eymeriosis because besides antibacterial substances it was necessary to use eymeriostatyks. Dedicated pathogens of stafilokokosis and pasteurellosis showed sensitivity to enrofloxacin and tylosin. Animals of one group was used brovaseptol complex preparation, the drug used in another 50% of tylosin and salikoks. The high therapeutic effect provided the use of biologics in animals of both groups. The use of tylosin were effective individually in combination with solikoks and the application of complex drug brovaseptol. A week after recovery animals were immunized rabbits all vaccine Pazorin-Ol, (Pasorin-Ol).

The results of the study geese pasteurellosis outbreaks in private zoo in the Poltava region. Diagnosis for pasteurellosis set comprehensively, taking into account epizootological features, clinical signs and pathological changes.The diagnosis was carried out comprehensively, taking into account the characteristics of epizootic, clinical signs and postmortem autopsy results. In determining the diagnosis were the results of microscopic and bacteriological research. To study the morphology of microorganisms strokes, the selected cultures were stained with alcoholic–aqueous methylene blue. We studied the sensitivity of selected microorganisms to certain antibiotics. After 24 hours of incubation in nutrient medium grew rounded, smooth, «wax» colonies of microorganisms.In smears of colonies that grew were found to IPA Pasteurella – typical bipolar coli. After obtaining a pure culture was carried determine the sensitivity of the selected pathogen to various antibacterial agents. For this we used disco–diffusion method. After the incubation, the Petri dish looked and measured in millimeters zone delay line growth of microorganisms.According to the results obtained, isolated Pasteurella culture revealed high sensitivity to tilozyn. This antibacterial agent caused stunted growth zone microflora 14–21 mm; High sensitivity also highlighted the culture was determined to enrofloxacin (zone of stunted growth – 16–24 mm) and gentamicin (zone of stunted growth – 18–24 mm) Moderately resistant culture was to kanamycin and chloramphenicol (zone growth retardation – 8–14 mm in accordance). To the rest of the drugs was highlighted microflora resistant, zone microflora growth delay ranged from 0 to 4 mm.The drug Enroksil 10% solution, based on enrofloxacin used orally in drinking water at the rate of 5 ml per 10 liters of water for three days. During treatment birds received only water containing the drug. The high sensitivity of microflora selected a number of antibacterial drugs and high efficiency of therapeutic measures can be explained by timely treatment zoo owner for help with diagnosis.