1. Suppression of ethanol and lipopolysaccharide-induced liver injury by extracts of Hydrangeae Dulcis Folium in rats.
- Author
-
Hashizume E, Nakagiri R, Shirai A, Kayahashi S, Yasushi S, and Kamiya T
- Subjects
- Alanine Transaminase blood, Animal Feed, Animals, Body Weight drug effects, Cell Line, Chemical and Drug Induced Liver Injury, Dietary Supplements, Dose-Response Relationship, Drug, Eating drug effects, Escherichia coli chemistry, Escherichia coli genetics, Ethanol toxicity, Female, Lipopolysaccharides toxicity, Liver drug effects, Liver enzymology, Liver pathology, Liver Diseases enzymology, Liver Diseases pathology, Macrophages metabolism, Mice, Silybum marianum chemistry, Rats, Rats, Sprague-Dawley, Tumor Necrosis Factor-alpha biosynthesis, Ethanol antagonists & inhibitors, Hydrangeaceae chemistry, Lipopolysaccharides antagonists & inhibitors, Liver Diseases prevention & control, Plant Extracts pharmacology
- Abstract
In female SD rats that were injected with 4 g/kg BW ethanol p.o. followed by a 5 mg/kg BW lipopolysaccharide (LPS) i.v. injection, serum glutamic pyruvic transaminases (GPT) activity increased to about eight times that of normal rats. In this model, rats that had been fed a diet containing 1% Hydrangeae Dulcis Folium (HDF) extracts for fifteen days showed significantly lower serum GPT activity (380.0+/-58.2 IU/l) than the control group (3527.0+/-774.1 IU/l). HDF's efficacy was far superior to milk thistle in this model (2950.0+/-915.9 IU/l). When mouse macrophages were treated with HDF extracts at 50 microg/ml, TNF-alpha production induced by LPS was suppressed to about 10% of the control. Rat serum TNF-alpha levels induced by LPS was decreased to 58.7% of the control by administering 1000 mg/kg BW HDF extract p.o. These results indicate that HDF prevents alcohol-induced liver injury through the inhibition of TNF-alpha production.
- Published
- 2003
- Full Text
- View/download PDF