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4. Identification of newly developed advanced schistosomiasis with MALDI-TOF mass spectrometry and ClinProTools analysis

Author

Huang Yuzheng, Xu Yongliang, Huang Yi, Sun Fang, Tian Haisong, Hu Nannan, Shi Liang, and Hua Haiyong

Subjects
newly developed advanced schistosomiasis, MALDI-TOF mass spectrometry, ClinProTools, proteomic detection pattern, Infectious and parasitic diseases, RC109-216
Abstract

Cases of newly developed advanced schistosomiasis (NDAS) have occurred in areas where schistosomiasis transmission has been blocked for more than 25 years. The causes and pathogenesis of NDAS are still unknown. Diagnosis of NDAS relies on historical investigation and clinical symptoms, such as liver fibrosis, hepatic ascites and abnormal biochemical indexes in serum. It is important but difficult at this stage to develop a new tool for early screening and rapid diagnosis. In this study, serum peptides from thirty patients with NDAS and thirty healthy controls were captured with weak cation exchange magnetic beads, and subjected to MALDI-TOF mass spectrometry and ClinProTools analysis. Eleven peaks with m/z 924, 2661, 2953, 2991, 3241, 3884, 5337, 5905, 5943, 7766 and 9289 were decreased and three peaks with m/z 1945, 2082 and 4282 were increased in the NDAS group. The proteomic detection pattern (PDP) was established with 14 different peptide peaks, and its sensitivity and specificity were investigated with a blind test. The peptide mass fingerprints of sera from 50 NDAS patients and 100 healthy controls were double-blind subjected to the PDP method, and 50 patients and 92 healthy controls were classified as NDAS and healthy separately, which showed 100% sensitivity and 92% specificity. Our results showed that the PDP could be a new and useful method to detect NDAS.

Published
2019
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6. The Deletion of IL-17A Enhances Helicobacter hepaticus Colonization and Triggers Colitis

Author

Zhu,Liqi, Wu,Zhihao, Zhu,Chen, Yin,Jun, Huang,Yuzheng, Feng,Jie, Zhang,Quan, Zhu,Liqi, Wu,Zhihao, Zhu,Chen, Yin,Jun, Huang,Yuzheng, Feng,Jie, and Zhang,Quan

Abstract

Liqi Zhu,1,2 Zhihao Wu,1,2 Chen Zhu,1,2 Jun Yin,1,2 Yuzheng Huang,3,4 Jie Feng,5 Quan Zhang1,2 1Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, People’s Republic of China; 2Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, 225009, People’s Republic of China; 3National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province, 214064, People’s Republic of China; 4Public Health Research Center, Jiangnan University, Wuxi, Jiangsu Province, 214122, People’s Republic of China; 5Shanghai Laboratory Animal Research Center, Shanghai Quality Monitoring Center of Laboratory Animals, Shanghai, 201203, People’s Republic of ChinaCorrespondence: Quan Zhang, Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, People’s Republic of China, Tel +86 138-1584-1244, Email zquan@yzu.edu.cnObjective: IL-17 is a key regulator of the inflammatory response, and as such, it is involved in the constraint and clearance of pathogens. The mechanism of IL-17 in the pathogenesis of inflammatory bowel disease (IBD) caused by microbial infection is still unclear. Helicobacter hepaticus infection can induce colitis in many mouse strains, and thus, it has been widely used in the study of IBD pathogenesis.Methods: In this study, male C57BL/6, BALB/c, Il-10−/−, and Il-17a−/− mice were infected with H. hepaticus for several weeks. Histopathology, H. hepaticus colonization and distribution, expression of inflammatory cytokines and lysozyme, and distribution of mucus in proximal colon were examined.Results: The colonic colonization of H. hepaticus was abnormally high in Il-17a−/− mice. H. hepaticus infection caused on

Published
2022

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