Search

Pseudorabies virus (PRV) is one of the causative agents of common infectious diseases in swine herds. Enterococcus faecium is a probiotic belonging to the group of lactic acid bacteria and has excellent immunomodulatory effects. Vaccine immunization is an important approach to prevent animal diseases in the modern farming industry, and good immunization outcomes can substantially reduce the damage caused by pathogens to animals, improve the quality of animals' lives, and reduce economic losses. In the present study, we showed that inactivated E. faecium and inactivated PRV when co-injected intravenously significantly reduced the mortality of mice after inoculation with PRV. The inactivated E. faecium + inactivated PRV intravenous injection group induced more production of Th cells and Tc cells. Additionally, the inactivated E. faecium + inactivated PRV intravenous injection group showed higher concentrations of cytokines (IFN-γ and IL-10) and induced higher antibody production. Thus, the co-injection of inactivated E. faecium and inactivated PRV could remarkably prevent and control the lethality of PRV infection in mice, which is a critical finding for vaccination and clinical development. [ABSTRACT FROM AUTHOR]

Today's desktop rendering platforms typically use GPUs, which have become the most powerful computing chip to meet the growing visual needs, especially in ray tracing. However, ray tracing is challenging for mobile platforms because mobile GPUs need to accommodate insufficient computing power, hardware resources, and memory bandwidth. This paper presents a novel architecture for the mobile domain called Mobile Multiple stacks Ray Tracing (MMsRT). The most complicated calculations in ray tracing are completed through lightweight embedded design. MMsRT has three key features: First, we set multiple stacks to ensure multiple rays are parallel in the system. Second, it sets a stack cache to store the data in stacks when the storage space of multiple stacks is insufficient. Third, we adopt the data prefetching mechanism to set caches to improve the cache hit rate and performance. An accurate simulator test proves that our design can be applied to mobile devices. We calculate the performance of about 82.9 Million Rays Per Second (MRPS), the chip area is about 0.856 mm2, and 96.85 MRPS/mm2. [ABSTRACT FROM AUTHOR]

Hardware prefetching has always been a crucial mechanism to improve processor performance. However, an efficient prefetch operation requires a guarantee of high prefetch accuracy; otherwise, it may degrade system performance. Prior studies propose an adaptive priority controlling method to make better use of prefetch accesses, which improves performance in two-level cache systems. However, this method does not perform well in a more complex memory hierarchy, such as a three-level cache system. Thus, it is still necessary to explore the efficiency of prefetch, in particular, in complex hierarchical memory systems. In this paper, we propose a composite hierarchy-aware method called CHAM, which works at the middle level cache (MLC). By using prefetch accuracy as an evaluation criterion, CHAM improves the efficiency of prefetch accesses based on (1) a dynamic adaptive prefetch control mechanism to schedule the priority and data transfer of prefetch accesses across the cache hierarchical levels in the runtime and (2) a prefetch efficiency-oriented hybrid cache replacement policy to select the most suitable policy. To demonstrate its effectiveness, we have performed extensive experiments on 28 benchmarks from SPEC CPU2006 and two benchmarks from BioBench. Compared with a similar adaptive method, CHAM improves the MLC demand hit rate by 9.2% and an improvement of 1.4% in system performance on average in a single-core system. On a 4-core system, CHAM improves the demand hit rate by 33.06% and improves system performance by 10.1% on average. [ABSTRACT FROM AUTHOR]

Multicore designs have become the dominant organization for future high performance microprocessors. Instead of increasing cache sizes, clock frequencies, pipeline depths or register file (RF) ports, multicore designs tend to make each processor core simple but highly efficient. This new dimension for improving performance and power efficiency in multicore requires us to rethink processor architecture. Multiply-accumulate (MAC) operation is such a performance improvement technique that needs to be reviewed. MAC operation is the fundamentals of many DSP and multimedia applications, but it tends to be awkward to implement in an orthogonal instruction set architecture (ISA) because of operand bandwidth problem, instruction encoding problem, and hardware cost problem. So a big question is that whether we should support MAC or not in high-efficiency processor designs? This paper does a comparative study on this question and introduce data bandwidth relaxing techniques to eliminate narrow bandwidth provided by two-port RFs. The trade-off are also made to solve the instruction coding and hardware cost problem. So, the new design wisdom becomes that if you support multiply (MUL) operation, then support MAC operation. [ABSTRACT FROM AUTHOR]

This paper proposes dynamic streamization model execution (DSME), a dynamic vectorization technique for single instruction multiple data (SIMD) engines on multicore architectures. The technique uses stream model as intermediate representation for programs to optimize the combination of computation and memory accesses of SIMD engines in general-purpose (GP) designs. DSME allows the dynamic placement of computations on different cores when they are not in use to utilize multiple SIMD engines. This study also discusses hardware extensions to existing GP processor designs as well as related compiler extensions that use the special hardware components. Our extensive experiments demonstrate that performance gains of DSME can be achieved. [ABSTRACT FROM PUBLISHER]

This study explored and investigated how zearalenone (ZEA) affects the morphology of small intestine and the distribution and expression of ghrelin and proliferating cell nuclear antigen (PCNA) in the small intestine of weaned gilts. A total of 20 weaned gilts (42-day-old, D × L × Y, weighing 12.84 ± 0.26 kg) were divided into the control and ZEA groups (ZEA at 1.04 mg/kg in diet) in a 35-d study. Histological observations of the small intestines revealed that villus injuries of the duodenum, jejunum and ileum, such as atrophy, retardation and branching dysfunction, were observed in the ZEA treatment. The villi branch of the ileum in the ZEA group was obviously decreased compared to that of the ileum, jejunum and duodenum, and the number of lymphoid nodules of the ileum was increased. Additionally, the effect of ZEA (1.04 mg/kg) was decreased by the immunoreactivity and distribution of ghrelin and PCNA in the duodenal and jejunal mucosal epithelial cells. Interestingly, ZEA increased the immunoreactivity of ghrelin in the ileal mucosal epithelial cells and decreased the immunoreactivity expression of PCNA in the gland epithelium of the small intestine. In conclusion, ZEA (1.04 mg/kg) had adverse effects on the development and the absorptive capacity of the villi of the intestines; yet, the small intestine could resist or ameliorate the adverse effects of ZEA by changing the autocrine of ghrelin in intestinal epithelial cells. [ABSTRACT FROM AUTHOR]

Deoxynivalenol (DON) is a mycotoxin found in grains that poses a potential threat to human and animal health, and the gastrointestinal tract is the primary target organ. There are few studies focused on the toxicology of DON to rabbits, especially on the relation among DON, microbiota, and the gut-associated lymphoid tissue. A total of 30 weaned rabbits (35 d) were evenly divided into the control group and DON group (1.5 mg/kg bodyweight (BW)) based on their body weight. After a 24-day trial, the ultrastructures of the sacculus rotundus and vermiform appendix were observed using a scanning electron microscope and transmission electron microscopy. The morphology and microflora in the ileum, caecum, and colon were also examined. The results proved that the ultrastructure of the sacculus rotundus and vermiform appendix, as well as the integrity of the intestinal barrier (especially for the ileum), were impaired after DON was administrated to the rabbits. Compared to the control group, the relative abundance and diversity of the microflora decreased in all three intestinal segments in the DON group, particularly in the ileum and caecum. In conclusion, the toxic effect of DON on weaned rabbits may be performed by destroying the structure of the sacculus rotundus and vermiform appendix, as well as affecting the structure and diversity of the intestinal flora. [ABSTRACT FROM AUTHOR]

Deoxynivalenol (DON) is a potential pathogenic factor to humans and animals, and intestinal tract is the primary target organ of DON. Data concerning the effects of DON on rabbits are scarce, especially for weaning rabbits. In this study, 45 weaning rabbits (35 d) were randomly and equally assigned into three groups. Group A was fed basic diet, while groups B and C were added DON at 0.5 mg/kg BW/d and 1.5 mg/kg BW/d, respectively, based on the basic diet. The experiment lasted for 24 days and the intestinal morphology, expression, and distribution of several cytokines in intestinal segments have been examined. The results indicated that ADG decreased while F/G increased significantly compared with the control group after DON added at 1.5 mg/kg BW/d. Some of the morphometric parameters (villi length, crypt depth, and goblet cells density) changed after DON was added. Meanwhile, the concentration as well as the expression levels of relative protein and mRNA of IL-1β, IL-2, IL-6, and IL-8 increased significantly. The immunohistochemistry results illustrated that the quantity and distribution of positive cells of inflammatory cytokines were changed after DON was added. In conclusion, the addition of DON damaged the intestinal morphology and changed the distribution and expression of inflammatory cytokines. The toxic effect depended on the dosage of DON. [ABSTRACT FROM AUTHOR]

Zearalenone (ZEN), a non-steroidal Fusarium graminearum with an estrogen effect, can cause damage to the gastrointestinal tract, immune organs, liver, and reproductive system. Further analysis of the mechanism of ZEN has become an important scientific issue. We have established in vivo and in vitro models of ZEN intervention, used AMPK/mTOR as a targeted pathway for ZEN reproductive toxicity, and explored the molecular mechanism by which ZEN may induce uterine hypertrophy in weaned piglets. Our study strongly suggested that ZEN can activate the phosphorylation of AMPK in uterine endometrial epithelium cells, affect the phosphorylation level of mTOR through TSC2 and Rheb, induce autophagy, upregulate the expression of proliferative genes PCNA and BCL2, downregulate the expression of apoptotic gene BAX, promote uterine endometrial epithelium cells proliferation, and ultimately lead to thickening of the endometrial and myometrium, increased density of uterine glands, and induce uterine hypertrophy. [ABSTRACT FROM AUTHOR]

Recent support for unified memory and demand paging has improved graphics processing unit (GPU) programmability and enabled memory oversubscription. However, this support introduces high overhead when page faults occur. Therefore, when the GPU memory fills to capacity, an important issue is how to select eviction candidates. The widely used policy, LRU, and the advanced replacement policies, RRIP and CLOCK-Pro, suffer from inefficiency when dealing with thrashing access patterns. They also incur significant overhead due to managing metadata at page level. In this article, we propose hierarchical page eviction (HPE), a new replacement policy for GPUs with unified memory. Aided by page walk hit information, HPE manages a page set chain dynamically. It uses statistics to classify applications into three categories and selects an appropriate eviction strategy for each category. It also applies dynamic adjustment to switch the eviction strategy when necessary. The simulation results show that, on average, HPE achieves 1.34 × and 1.16 × speedup (up to 2.81 × ) over LRU when the oversubscription rate is 75% and 50%, respectively. HPE also outperforms RRIP and CLOCK-Pro. [ABSTRACT FROM AUTHOR]

The introduction of unified memory and demand paging has simplified programming of graphics processing units (GPUs). It has also enabled oversubscribing the memory for a GPU. However, the overhead of page management makes page faults a performance bottleneck. Sometimes the page eviction policy is unable to mitigate performance slowdown caused by page faults and memory oversubscription. On average, eviction policies such as Random and CAR are not competitive with a traditional least recently used (LRU) policy. Other policies, such as CLOCK-Pro, are designed to overcome challenges with LRU, but they only achieve limited speedup. Even enhancing LRU with page walk hit information does not lead to notable performance improvement. Based on these observations, we propose optimization opportunities to mitigate performance degradation caused by page faults and memory oversubscription. These optimization opportunities include an effective page eviction policy that retains LRU's advantages while addressing LRU's inability to deal with thrashing access patterns, page prefetch and pre-eviction, memory-aware throttling, and capacity compression. [ABSTRACT FROM AUTHOR]

Researches have shown that dietary zearalenone (ZEA) caused oxidative stress in the liver and reproductive organs of postweaning gilts. However, information on the effects of ZEA on oxidative stress of the small intestine in the piglets is limited. The objective of this study was to determine the effects of ZEA exposure on oxidative stress, the Kelch-like erythroid cell-derived protein with CNC homology (ECH)–associated protein 1 (Keap1)–nuclear factor erythroid 2–related factor 2 (Nrf2) signaling pathway and on immunohistochemistry of the jejunum in postweaning gilts. A 35-d feeding experiment using 40 postweaning gilts (Landrace × Yorkshire × Duroc) with an average BW of 14.01 ± 0.86 kg in 4 groups fed corn–soybean meal-based diets containing 0, 0.5, 1.0, and 1.5 mg ZEA/kg was conducted. The jejunum was obtained at the end of the experiment and used for analyses. The results showed that the activities of total superoxide dismutase and glutathione peroxidase and the relative expressions of Keap1 mRNA and protein in the jejunum linearly and quadratically decreased (P < 0.05) with increasing concentrations of ZEA in the diets. The malondialdehyde content, the integrated optical density of Nrf2 and glutathione peroxidase 1 (GPX1), and the relative expressions of Nrf2, GPX1, quinone oxidoreductase 1 (NQO1), and modifier subunit of glutamate-cysteine ligase (GCLM) mRNA and proteins linearly and quadratically increased (P < 0.05) with increasing levels of ZEA. Immunohistochemical analysis showed that Nrf2 and GPX1 immunoreactivity was enhanced by the ZEA treatments, and block localization of yellow and brown immunoreactive substances in the jejunum was observed with increasing levels of ZEA. The results suggest that ingested ZEA induced oxidative stress in the jejunum in postweaning gilts through upregulation of the Keap1–Nrf2 signaling pathway and downstream target genes NQO1, HO1, and GCLM, indicating the important role of the Keap1–Nrf2 signaling pathway in oxidative stress induced by ZEA in the jejunum of the postweaning piglets. [ABSTRACT FROM AUTHOR]

Memory access scheduling schemes, often performed in memory controllers, have a marked impact on alleviating the heavy burden placed on memory systems of GPGPUs. Existing out-of-order scheduling schemes, like FR-FCFS, improve memory access efficiency by reordering memory request sequences at the destination. Their effectiveness, however, is at the expense of complex logics and high power consumption. In this paper, we propose a NoC-side memory access scheduling based on the key insight that the transmission of on-chip networks is the dominating factor in destroying the row access locality and causing poor memory access efficiency. With appropriate NoC-side optimization, the straight-forward in-order scheduling can be used in memory controllers to simplify scheduling logics and alleviate the tight power envelope. Moreover, we introduce several light-weight optimizations to further improve the system performance. Experimental results on memory-intensive applications show that, comparing with FR-FCFS, our proposed scheme increases the overall system performance by 10.5%, reduces the power consumption by 20% and improves the energy efficiency by 36.9%. [ABSTRACT FROM AUTHOR]

This paper presents a target tracking algorithm based on 4D millimeter-wave radar point cloud information for autonomous driving applications, which addresses the limitations of traditional 2 + 1D radar systems by using higher resolution target point cloud information that enables more accurate motion state estimation and target contour information. The proposed algorithm includes several steps, starting with the estimation of the ego vehicle's velocity information using the radial velocity information of the millimeter-wave radar point cloud. Different clustering suggestions are then obtained using a density-based clustering method, and correlation regions of the targets are obtained based on these clustering suggestions. The binary Bayesian filtering method is then used to determine whether the targets are dynamic or static targets based on their distribution characteristics. For dynamic targets, Kalman filtering is used to estimate and update the state of the target using trajectory and velocity information, while for static targets, the rolling ball method is used to estimate and update the shape contour boundary of the target. Unassociated measurements are estimated for the contour and initialized for the trajectory, and unassociated trajectory targets are selectively retained and deleted. The effectiveness of the proposed method is verified using real data. Overall, the proposed target tracking algorithm based on 4D millimeter-wave radar point cloud information has the potential to improve the accuracy and reliability of target tracking in autonomous driving applications, providing more comprehensive motion state and target contour information for better decision making. [ABSTRACT FROM AUTHOR]

Binary64 arithmetic is rapidly becoming inadequate to cope with today's large-scale computations due to an accumulation of errors. Therefore, binary128 arithmetic is now required to increase the accuracy and reliability of these computations. At the same time, an obvious trend emerging in modern processors is to extend their instruction sets by allowing single instruction multiple data (SIMD) execution, which can significantly accelerate the data-parallel applications. To address the combined demands mentioned above, this paper presents the architecture of a low-cost binary128 floating-point fused multiply add (FMA) unit with SIMD support. The proposed FMA design can execute a binary128 FMA every other cycle with a latency of four cycles, or two binary64 FMAs fully pipelined with a latency of three cycles, or four binary32 FMAs fully pipelined with a latency of three cycles. We use two binary64 FMA units to support binary128 FMA which requires much less hardware than a fully pipelined binary128 FMA. The presented binary128 FMA design uses both segmentation and iteration hardware vectorization methods to trade off performance, such as throughput and latency, against area and power. Compared with a standard binary128 FMA implementation, the proposed FMA design has 30 percent less area and 29 percent less dynamic power dissipation. [ABSTRACT FROM PUBLISHER]

In this study, we aimed to assess the effect of diet ZEA on serum hormones, the location and expression of estrogen receptor ERα/β and progesterone receptor (PR) of the uterus in weaned piglets and to reveal the mechanism underneath. A total of 40 healthy weaned gilts were randomly allocated to basal diet supplemented with 0 (Control), 0.5 (ZEA0.5), 1.0 (ZEA1.0) and 1.5 (ZEA1.5) mg ZEA/kg and fed individually for 35 days. Meanwhile, the porcine endometrial epithelial cells (PECs) were incubated for 24 h with ZEA at 0 (Control), 5 (ZEA5), 20 (ZEA20) and 80 (ZEA80) μmol/L, respectively. The results showed that nutrient apparent digestibility (CP and GE), nutrient apparent availability (ME/GE, BV and NPU), the uterine immunoreactive integrated optic density (IOD), relative mRNA and protein expression of ER-α, ER-β and PR and the relative mRNA and protein expression of ER-α and ER-β in PECs all increased linearly (p < 0.05) with ZEA. Collectively, ZEA can interfere with the secretion of some reproductive hormones in the serum and promote the expression of estrogen/progesterone receptors in the uterus and PECs. All these indicate that ZEA may promote the development of the uterus in weaned gilts through estrogen receptor pathway. [ABSTRACT FROM AUTHOR]

Simple Summary: Paraformic acid (PFA) is a hyperpolymer formed by polymerization of formic acid monomers, and PFA has a lower pungent smell and corrosiveness to the gastrointestinal tract compared with formic acid. The liver is a crucial metabolic organ, playing a momentous role in nutrients biosynthesis and metabolism, defense against bacterial invasion, and endotoxin clearance. However, in the condition of intensive poultry husbandry, the broiler's liver is susceptible to inflammatory damage. Our results showed that PFA supplemented to the diet improved growth performance, inhibited inflammatory response, and benefited liver protection, which provided a direction for the use of PFA in broiler production. The current study aimed to explore the effects of supplementing paraformic acid (PFA) into broilers' diet on growth performance, inflammatory responses, and liver protection. A total of 567 healthy one-day-old broilers were used in a 42-d study, and they were randomized into three groups. Broilers were fed a basal diet (CON group) or the basal diet supplemented with either 50 mg/kg aureomycin (AB group) or 1000 mg/kg PFA (PFA group). The results showed that the PFA and AB groups had a higher feed conversion rate than the CON group from day 21 to 42 (p < 0.05). Dietary PFA or aureomycin supplementation decreased serum levels of interleukin (IL)-1β, IL-6, IL-10, alanine transaminase, diamine oxidase, and D-lactate, and significantly increased serum concentrations of immunoglobulin (Ig) A, IgM, and complement C4 (p < 0.05). Moreover, dietary PFA or aureomycin supplementation decreased hepatic levels of caspase-1, NOD-like receptor family pyrin domain containing 3 (NLRP3), tumor necrosis factor-alpha, IL-6, and IL-18, as well as NF-κB mRNA expression (p < 0.05). Above all, PFA supplementation into the broilers' diet improved growth performance, inhibited inflammatory responses, and benefited liver protection. The protective effects of PFA on the liver might be related to inhibition of caspase-1-induced pyroptosis via inactivating the NF-κB/NLRP3 inflammasome axis in broiler chickens. [ABSTRACT FROM AUTHOR]

Zearalenone (ZEN), also known as the F-2 toxin, is a common contaminant in cereal crops and livestock products. This experiment aimed to reveal the changes in the proteomics of ZEN-induced intestinal damage in weaned piglets by tandem mass spectrometry tags. Sixteen weaned piglets either received a basal diet or a basal diet supplemented with 3.0 mg/kg ZEN in a 32 d study. The results showed that the serum levels of ZEN, α-zearalenol, and β-zearalenol were increased in weaned piglets exposed to ZEN (p < 0.05). Zearalenone exposure reduced apparent nutrient digestibility, increased intestinal permeability, and caused intestinal damage in weaned piglets. Meanwhile, a total of 174 differential proteins (DEPs) were identified between control and ZEN groups, with 60 up-regulated DEPs and 114 down-regulated DEPs (FC > 1.20 or <0.83, p < 0.05). Gene ontology analysis revealed that DEPs were mainly involved in substance transport and metabolism, gene expression, inflammatory, and oxidative stress. The Kyoto Encyclopedia of Genes and Genomes analysis revealed that DEPs were significantly enriched in 25 signaling pathways (p < 0.05), most of which were related to inflammation and amino acid metabolism. Our study provides valuable clues to elucidate the possible mechanism of ZEN-induced intestinal injury. [ABSTRACT FROM AUTHOR]

The aim of this study was to explore the effect of zearalenone (ZEA) exposure on uterine development in weaned gilts by quantitative proteome analysis with tandem mass spectrometry tags (TMT). A total of 16 healthy weaned gilts were randomly divided into control (basal diet) and ZEA3.0 treatments groups (basal diet supplemented with 3.0 mg/kg ZEA). Results showed that vulva size and uterine development index were increased (p < 0.05), whereas serum follicle stimulation hormone, luteinizing hormone and gonadotropin-releasing hormone were decreased in gilts fed the ZEA diet (p < 0.05). ZEA, α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) were detected in the uteri of gilts fed a 3.0 mg/kg ZEA diet (p < 0.05). The relative protein expression levels of creatine kinase M-type (CKM), atriopeptidase (MME) and myeloperoxidase (MPO) were up-regulated (p < 0.05), whereas aldehyde dehydrogenase 1 family member (ALDH1A2), secretogranin-1 (CHGB) and SURP and G-patch domain containing 1 (SUGP1) were down-regulated (p < 0.05) in the ZEA3.0 group by western blot, which indicated that the proteomics data were dependable. In addition, the functions of differentially expressed proteins (DEPs) mainly involved the cellular process, biological regulation and metabolic process in the biological process category. Some important signaling pathways were changed in the ZEA3.0 group, such as extracellular matrix (ECM)-receptor interaction, focal adhesion and the phosphoinositide 3-kinase–protein kinase B (PI3K-AKT) signaling pathway (p < 0.01). This study sheds new light on the molecular mechanism of ZEA in the uterine development of gilts. [ABSTRACT FROM AUTHOR]

Simple Summary: Tannic acid (TA) is a hydrophilic polyphenolic compound present in most plants. Galla chinensis, a traditional Chinese medicine, is widely available and used in China, and TA is one of the main bioactive components of Galla chinensis. Until now, there is little information available in the scientific literature on the evaluation of the application of Galla chinensis TA in broiler production. A healthy and well-developed intestine is critical in absorbing nutrients and serves as a vital barrier against pathogen invasion. However, the intestine of the broiler is vulnerable to inflammatory damage under intensive poultry farming conditions. Therefore, we aimed to investigate the effects of adding Galla chinensis TA to the diet of broiler chickens on intestinal development and health, and highlight the application of TA sourced from Galla chinensis as a potential feed additive in poultry production. Our results showed that 300 mg/kg microencapsulated TA from Galla chinensis supplementation benefited to improve intestinal development, which might be attributed to the suppression of inflammatory responses via blockage of NF-κB in broiler chickens, and provide guidance for the application of Galla chinensis TA in poultry production. The objective of this study was to investigate the effects of adding tannic acid (TA) extracted from Galla chinensis to the diet of broiler chickens on intestinal development. A total of 324 healthy 1-day-old broilers were used in a 42 d study, and divided into two treatment groups at random (six replicates per group). Broilers were either received a basal diet or a basal diet supplemented with 300 mg/kg microencapsulated TA extracted from Galla chinensis. The results showed that dietary supplemented with 300 mg/kg TA from Galla chinensis improved intestinal morphology, promoted intestinal mucosal barrier integrity, and elevated mucosal expressions of nutrients transporters and tight junction protein CLDN3 in broilers. Besides, 300 mg/kg TA from Galla chinensis supplementation decreased the concentrations of inflammatory cytokines in serum and intestinal mucosa and reduced the mRNA expression of NF-κB in intestinal mucosa. Above all, supplementation of 300 mg/kg microencapsulated TA extracted from Galla chinensis showed beneficial effects in improving intestinal development, which might be attributed to the suppression of inflammatory responses via blockage of NF-κB in broiler chickens. These findings will support the use of TA sourced from Galla chinensis in poultry industry. [ABSTRACT FROM AUTHOR]

Huangjiu usually caused rapid-drunkenness and components such as β-benzyl ethanol (β-be), isopentanol (Iso), histamine (His), and phenethylamine (PEA) have been reported linked with intoxication. However, the destructive effect of these components on gut microbiota and liver is unclear. In this study, we found oral treatment of these components, especially His, stimulated the level of oxidative stress and inflammatory cytokines in liver and serum of mice. The gut microbiota community was changed and the level of lipopolysaccharide (LPS) increased significantly. Additionally, cellular pyroptosis pathway has been assessed and correlation analysis revealed a possible relationship between gut microbiota and liver pyroptosis. We speculated oral His treatment caused the reprogramming of gut microbiota metabolism, and increased LPS modulated the gut-liver interaction, resulting in liver pyroptosis, which might cause health risks. This study provided a theoretical basis for the effect of Huangjiu, facilitating the development of therapeutic and preventive strategies for related inflammatory disorders. [ABSTRACT FROM AUTHOR]

Traffic participant classification is critical in autonomous driving perception. Millimetre wave radio detection and ranging (RADAR) is a cost‐effective and robust means of performing this task in adverse traffic scenarios such as inclement weather (e.g. fog, snow, and rain) and poor lighting conditions. Compared to commercial two‐dimensional RADAR, the new generation of three‐dimensional (3D) RADAR can obtain height information about targets as well as their dense point clouds, greatly improving target classification capabilities. This study proposes a multi‐objective classification method for traffic participants based on 3D RADAR point clouds. First, a 22‐dimensional feature vector of the 3D RADAR point cloud distribution was extracted to describe the shape, discrete, Doppler, and reflection intensity features of the targets. Then, dynamic and static datasets containing five classes of targets were produced, creating a 10k frame. Extensive experiments were conducted to build machine learning classifiers. The experimental results show that the trained classifiers can achieve over 92% classification accuracy when the targets are classified into five groups and over 95% classification accuracy when the targets are classified into four groups. The proposed method can guide the design of safer and more efficient intelligent driving systems. [ABSTRACT FROM AUTHOR]

Fumonisin B1 (FB1) is the most common food-borne mycotoxin produced by the Fusarium species, posing a potential threat to human and animal health. Pigs are more sensitive to FB1 ingested from feed compared to other farmed livestock. Enzymatic degradation is an ideal detoxification method that has attracted much attention. This study aimed to explore the functional characteristics of the carboxylesterase FumDSB in growing pigs from the perspective of brain–gut regulation. A total of 24 growing pigs were divided into three groups. The control group was fed a basal diet, the FB1 group was supplemented with FB1 at 5 mg/kg feed, and the FumDSB group received added FumDSB based on the diet of the FB1 group. After 35 days of animal trials, samples from the hypothalamus and jejunum were analyzed through HE staining, qRT-PCR and immunohistochemistry. The results demonstrated that the ingestion of FB1 can reduce the feed intake and weight gain of growing pigs, indicating that several appetite-related brain-gut peptides (including NPY, PYY, ghrelin and obestatin, etc.) play important roles in the anorexia response induced by FB1. After adding FumDSB as detoxifying enzymes, however, the anorexia effects of FB1 were alleviated, and the expression and distribution of the corresponding brain-gut peptides exhibited a certain degree of regulation. In conclusion, the addition of FumDSB can reduce the anorexia effects of FB1 by regulating several brain-gut peptides in both the hypothalamus and the jejunum of growing pigs. [ABSTRACT FROM AUTHOR]

Adaptive routing algorithms can improve performance by balancing load across network channels in the presence of non-uniform traffic patterns. However, out-of-order packets can be introduced due to multi-path transmission of adaptive routing. With out-of-order transmission in the network, packets need to be reordered at the destination before being absorbed. Increasing network size with adaptive routing makes the time when a packet arrives at the destination extremely uncertain, which requires a large buffer to reorder the packets and this can exceed design space. Therefore, the challenge is to balance the trade-off between multi-path transmission and packet reordering. In this paper, we propose a novel packet reordering metric-OOD to quantify the degree of out-of-order. To minimize the OOD of packets, we propose DancerFly, an order-aware network-on-chip router that mitigates out-of-order packets caused by adaptive routing. DancerFly achieves this goal by providing two-level reordering. First, it performs in-buffer reordering by reordering packets queuing in the input buffer. Second, packets from different input ports are reordered before traversing through the router. We evaluate our design and the results show that the OOD can be reduced by 36.3% with comparable performance to the baseline. [ABSTRACT FROM AUTHOR]

High performance implementation of matrix multiplication is essential for scientific computing. The memory access procedure is quite possible to be the bottleneck of matrix multiplication. The widely used GotoBLAS GEMM implementation divides the integral matrix into several partitions to be assigned to different cores for parallelization. Traditionally, each core deploys a DMA transfer to access its own partition in the DRAM memory. However, deploying an independent DMA transfer for each core cannot efficiently exploit the inter-core locality. Also, multiple concurrent DMA transfers interfere with each other, further reducing the DRAM access efficiency. We observe that the same row of neighboring partitions is in the same DRAM page, which means that there is significant locality inherent in the address layout. We propose the coordinated DMA to efficiently exploit the locality. It invokes one transfer to serve all cores and moves data in a row-major manner to improve the DRAM access efficiency. Compared with a baseline design, the coordinated DMA improves the bandwidth by 84.8 percent and reduces DRAM energy consumption by 43.1 percent for micro-benchmarks. It achieves higher performance for the GEMM and Linpack benchmark. With much less hardware costs, the coordinated DMA significantly outperforms an out-of-order memory controller. [ABSTRACT FROM AUTHOR]

Zearalenone (ZEA) is an estrogenic toxin produced by Fusarium species, which is widely distributed and posed a great health risk to both humans and farm animals. Reproductive disorders associated with ZEA such as premature puberty, infertility and abortion have plagued the animal husbandry, but the molecular mechanism is unclear. Because transforming growth factor-β1 (TGF-β1) signaling pathway is involved in the proliferation and apoptosis of cells, proliferating cell nuclear antigen (PCNA), B-cell lymphoma/leukemia-2 (BCL-2) and BCL-2 associated X protein (BAX) that all play indispensable roles in the normal development of the uterus, it is hypothesized that ZEA induces reproductive disorders is closely related to the expression of these genes. The objective of this study was to assess the effects of dietary ZEA at the concentrations of 0.5 to 1.5 mg/kg on the mRNA and protein expression of these genes in the uteri of post-weaning gilts and to explore the possible molecular mechanism. Forty healthy post-weaning female piglets (Duroc × Landrace × Large White) aged 38 d were randomly allocated to basal diet supplemented with 0 (Control), 0.5 (ZEA0.5), 1.0 (ZEA1.0), or 1.5 (ZEA1.5) mg/kg purified ZEA, and fed for 35 d. Piglets were euthanized at the end of the experiment and samples were taken and subjected to immunohistochemistry, qRT-PCR and Western blot analyses. The relative mRNA expressions of PCNA, BCL-2 and Smad3 in the uteri of post-weaning gilts increased linearly (p < 0.05) and quadratically (p < 0.05) as ZEA concentration increased in the diet. The relative protein expressions of PCNA, BAX, BCL-2, TGF-β1, Smad3, and phosphorylated Smad3 (p-Smad3) in the uteri of post-weaning gilts increased linearly (p < 0.05) and quadratically (p < 0.001) with an increasing level of ZEA. The results showed that uterine cells in the ZEA (0.5-1.5 mg/kg) treatments were in a high proliferation state, indicating that ZEA could accelerate the proliferation of uteri and promote the development of the uteri. At the same time, the results suggested that ZEA activates the TGF-β1/Smad3 signaling pathway, suggesting it plays an important role in accelerating the development of the uterus. [ABSTRACT FROM AUTHOR]

To provide efficient, high-performance routing algorithms, a holistic approach should be taken. The key aspects of routing algorithm design include adaptivity, path selection strategy, VC allocation, isolation, and hardware implementation cost; these design aspects are not independent. The key contribution of this work lies in the design of a novel selection strategy, Destination-Based Selection Strategy (DBSS), which targets interference that can arise in many-core systems running consolidation workloads. In the process of this design, we holistically consider all aspects to ensure an efficient design. Existing routing algorithms largely overlook issues associated with workload consolidation. Locally adaptive algorithms do not consider enough status information to avoid network congestion. Globally adaptive routing algorithms attack this issue by utilizing network status beyond neighboring nodes. However, they may suffer from interference, coupling the behavior of otherwise independent applications. To address these issues, DBSS leverages both local and nonlocal network status to provide more effective adaptivity. More importantly, by integrating the destination into the selection procedure, DBSS mitigates interference and offers dynamic isolation among applications. Results show that DBSS offers better performance than the best baseline selection strategy and improves the energy-delay product for medium and high injection rates; it is well suited for workload consolidation. [ABSTRACT FROM PUBLISHER]

This study aims to investigate the effects of exposure to different dosages of zearalenone (ZEA) on cecal physical barrier functions and its mechanisms based on the TGF-β1/Smads signaling pathway in weaned piglets. Thirty-two weaned piglets were allotted to four groups and fed a basal diet supplemented with ZEA at 0, 0.15, 1.5, and 3.0 mg/kg, respectively. The results showed that 1.5 and 3.0 mg/kg ZEA damaged cecum morphology and microvilli, and changed distribution and shape of M cells. Moreover, 1.5 and 3.0 mg/kg ZEA decreased numbers of goblet cells, the expressions of TFF3 and tight junction proteins, and inhibited the TGF-β1/Smads signaling pathway. Interestingly, the 0.15 mg/kg ZEA had no significant effect on cecal physical barrier functions but decreased the expressions of Smad3, p-Smad3 and Smad7. Our study suggests that high-dose ZEA exposure impairs cecal physical barrier functions through inhibiting the TGF-β1/Smads signaling pathway, but low-dose ZEA had no significant effect on cecum morphology and integrity through inhibiting the expression of smad7. These findings provide a scientific basis for helping people explore how to reduce the toxicity of ZEA in feeds. [ABSTRACT FROM AUTHOR]

Abstract: Three-dimensional (3D) nanostructured CaWO4:Tb3+microspheres assembled by submicrospindles were synthesized via a mild sonochemical route from an aqueous solution of CaCl2, TbCl3 and Na2WO4 with the aid of surfactant Polyglycol 600 (PEG-600). The crystal structure and morphology of the as-prepared products were characterized by using X-ray diffraction (XRD) and field emission scanning electron microscopy (FE-SEM). Rietveld refinement was carried out on the XRD data. The results showed that the CaWO4:Tb3+nanoparticles can be formed without ultrasonic irradiation or addition of PEG-600. With continuously increasing irradiation time the submicrospindles and microspheres could be self-assembled. The central diameter and length of the submicrospindles are around 190 and 500nm, respectively. The 3D CaWO4:Tb3+nanostructured microspheres with diameter of 2–4μm were assembled by the submicrospindles. A possible formation mechanism for the 3D-structured CaWO4:Tb3+microspheres was proposed. The Photoluminescent (PL) properties of Tb3+ions in the nanostructured CaWO4 microspheres were studied. The energy transfer processes in CaWO4:Tb3+microspheres were analyzed. The electric dipole–dipole energy transfers related to 5D3 level were studied by inspecting the fluorescence decay of 5D3 level. The energy transfer critical distance was estimated. [Copyright &y& Elsevier]

Abstract: Flower-like Y2(MoO4)3:Dy3+ phosphors have been synthesized via a co-precipitation approach with the aid of β-cyclodextrin. The crystal structure and morphology of the phosphors were characterized by XRD (X-ray diffraction) and FE-SEM (field emission scanning electron microscopy), respectively. The excitation and emission properties of the phosphors were examined by fluorescence spectroscopy. The dependence of color coordinates on the Dy3+ doping concentration was analyzed. The energy transfer mechanism between Dy3+ ions was studied based on the Huang''s theory, I-H and Van Uitert''s models. It was concluded simultaneously from these three routes that the electric dipole–dipole interaction between Dy3+ ions is the main physical mechanism for the energy transfers between Dy3+. [Copyright &y& Elsevier]

Hand gesture recognition technology plays an important role in human-computer interaction and in-vehicle entertainment. Under in-vehicle conditions, it is a great challenge to design gesture recognition systems due to variable driving conditions, complex backgrounds, and diversified gestures. In this paper, we propose a gesture recognition system based on frequency-modulated continuous-wave (FMCW) radar and transformer for an in-vehicle environment. Firstly, the original range-Doppler maps (RDMs), range-azimuth maps (RAMs), and range-elevation maps (REMs) of the time sequence of each gesture are obtained by radar signal processing. Then we preprocess the obtained data frames by region of interest (ROI) extraction, vibration removal algorithm, background removal algorithm, and standardization. We propose a transformer-based radar gesture recognition network named RGTNet. It fully extracts and fuses the spatial-temporal information of radar feature maps to complete the classification of various gestures. The experimental results show that our method can better complete the eight gesture classification tasks in the in-vehicle environment. The recognition accuracy is 97.56%. [ABSTRACT FROM AUTHOR]

This study aims to investigate the effects of zearalenone (ZEA) on the localizations and expressions of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), gonadotropin releasing hormone (GnRH) and gonadotropin releasing hormone receptor (GnRHR) in the ovaries of weaned gilts. Twenty 42-day-old weaned gilts were randomly allocated into two groups, and treated with a control diet and a ZEA-contaminated diet (ZEA 1.04 mg/kg), respectively. After 7-day adjustment, gilts were fed individually for 35 days and euthanized for blood and ovarian samples collection before morning feeding on the 36th day. Serum hormones of E2, PRG, FSH, LH and GnRH were determined using radioimmunoassay kits. The ovaries were collected for relative mRNA and protein expression, and immunohistochemical analysis of FSHR, LHR, GnRH and GnRHR. The results revealed that ZEA exposure significantly increased the final vulva area (p < 0.05), significantly elevated the serum concentrations of estradiol, follicle stimulating hormone and GnRH (p < 0.05), and markedly up-regulated the mRNA and protein expressions of FSHR, LHR, GnRH and GnRHR (p < 0.05). Besides, the results of immunohistochemistry showed that the immunoreactive substances of ovarian FSHR, LHR, GnRH and GnRHR in the gilts fed the ZEA-contaminated diet were stronger than the gilts fed the control diet. Our findings indicated that dietary ZEA (1.04 mg/kg) could cause follicular proliferation by interfering with the localization and expression of FSHR, LHR, GnRH and GnRHR, and then affect the follicular development of weaned gilts. [ABSTRACT FROM AUTHOR]

It is desirable to maintain high accuracy and runtime efficiency at the same time in lane detection. However, due to the long and thin properties of lanes, extracting features with both strong discrimination and perception abilities needs a huge amount of calculation, which seriously slows down the running speed. Therefore, we design a more efficient way to extract the features of lanes, including two phases: (1) Local feature extraction, which sets a series of predefined anchor lines, and extracts the local features through their locations. (2) Global feature aggregation, which treats local features as the nodes of the graph, and builds a fully connected graph by adaptively learning the distance between nodes, the global feature can be aggregated through weighted summing finally. Another problem that limits the performance is the information loss in feature compression, mainly due to the huge dimensional gap, e.g., from 512 to 8. To handle this issue, we propose a feature compression module based on decoupling representation learning. This module can effectively learn the statistical information and spatial relationships between features. After that, redundancy is greatly reduced and more critical information is retained. Extensional experimental results show that our proposed method is both fast and accurate. On the Tusimple and CULane benchmarks, with a running speed of 248 FPS, F1 values of 96.81% and 75.49% were achieved, respectively. [ABSTRACT FROM AUTHOR]

Automotive millimeter-wave (MMW) radar is essential in autonomous vehicles due to its robustness in all weather conditions. Traditional commercial automotive radars are limited by their resolution, which makes the object classification task difficult. Thus, the concept of a new generation of four-dimensional (4D) imaging radar was proposed. It has high azimuth and elevation resolution and contains Doppler information to produce a high-quality point cloud. In this paper, we propose an object classification network named Radar Transformer. The algorithm takes the attention mechanism as the core and adopts the combination of vector attention and scalar attention to make full use of the spatial information, Doppler information, and reflection intensity information of the radar point cloud to realize the deep fusion of local attention features and global attention features. We generated an imaging radar classification dataset and completed manual annotation. The experimental results show that our proposed method achieved an overall classification accuracy of 94.9%, which is more suitable for processing radar point clouds than the popular deep learning frameworks and shows promising performance. [ABSTRACT FROM AUTHOR]

Intelligent transportation systems (ITSs) play an increasingly important role in traffic management and traffic safety. Smart cameras are the most widely used sensors in ITSs. However, cameras suffer from a reduction in detection and positioning accuracy due to target occlusion and external environmental interference, which has become a bottleneck restricting ITS development. This work designs a stable perception system based on a millimeter-wave radar and camera to address these problems. Radar has better ranging accuracy and weather robustness, which is a better complement to camera perception. Based on an improved Gaussian mixture probability hypothesis density (GM-PHD) filter, we also propose an optimal attribute fusion algorithm for target detection and tracking. The algorithm selects the sensors' optimal measurement attributes to improve the localization accuracy while introducing an adaptive attenuation function and loss tags to ensure the continuity of the target trajectory. The verification experiments of the algorithm and the perception system demonstrate that our scheme can steadily output the classification and high-precision localization information of the target. The proposed framework could guide the design of safer and more efficient ITSs with low costs. [ABSTRACT FROM AUTHOR]

Simple Summary: This study was conducted to assess the effects of Zearalenone (ZEA) exposure on the growth performance, small intestine disaccharidase, and antioxidant activities of weaned gilts. Twenty weaned gilts were randomly divided into control and ZEA treatment (1.04 mg/kg) groups. The data showed that 1.04 mg/kg ZEA in gilt's diet could reduce the activity of disaccharidase enzymes and induce oxidative stress in the small intestine. Therefore, ZEA may induce intestinal injury by oxidative stress, or induce oxidative stress through intestinal injury, thus reducing the effect of animals on nutrient absorption. Zearalenone (ZEA) is a secondary metabolite with estrogenic effects produced by Fusarium fungi and mainly occurs as a contaminant of grains such as corn and wheat. ZEA, to which weaned gilts are extremely sensitive, is the main Fusarium toxin detected in corn–soybean meal diets. Our aim was to examine the effects of ZEA on the growth performance, intestinal disaccharidase activity, and anti-stress capacity of weaned gilts. Twenty 42-day-old healthy Duroc × Landrace × Large White weaned gilts (12.84 ± 0.26 kg) were randomly divided into control and treatment (diet containing 1.04 mg/kg ZEA) groups. The experiment included a 7-day pre-trial period followed by a 35-day test period, all gilts were euthanized and small intestinal samples were collected and subjected to immunohistochemical and western blot analyses. The results revealed that inclusion of 1.04 mg/kg ZEA in the diet significantly reduced the activities of lactase, sucrase, and maltase in the duodenum, jejunum, and ileum of gilts. Similarly, the activities of superoxide dismutase and glutathione peroxidase in the duodenum, jejunum, and ileum, and activities of catalase in the jejunum and ileum were reduced (p < 0.05). Conversely, the content of malondialdehyde in the duodenum, jejunum, and ileum, and the integrated optical density (IOD), IOD in single villi, and the mRNA and protein expression of heat shock protein 70 (Hsp70) were significantly increased (p < 0.05). The results of immunohistochemical analyses revealed that the positive reaction of Hsp70 in the duodenum, jejunum, and ileum of weaned gilts was enhanced in the ZEA treatment, compared with the control. The findings of this study indicate the inclusion of ZEA (1.04 mg/kg) in the diet of gilts reduced the activity of disaccharidase enzymes and induced oxidative stress in the small intestine, thereby indicating that ZEA would have the effect of reducing nutrient absorption in these animals. [ABSTRACT FROM AUTHOR]

Accumulating evidences indicate that plant extracts and probiotics are effective antioxidant substitutes which play important roles in animal production. However, the comparative study of the mechanism underlying the antioxidant property of Illicium verum extracts (IVE) and probiotics with added glucose oxidase (PGO) on piglets remains to be explored. This study evaluated the difference and the interaction effect of IVE and PGO on serum, liver, and jejunum antioxidant capacity of weaned piglets. A total of 32 weaned piglets (Duroc × Landrace × Yorkshire) at the age of 28 d with an average body weight of 14.96 ± 0.32 kg were randomly divided into four treatments with eight replicates per treatment in a 2 × 2 factorial arrangement. Treatments included basal diet (IVE−PGO−), basal diet + 1,000 mg/kg PGO (IVE−PGO+), basal diet + 500 mg/kg IVE (IVE+PGO−), and basal diet + 500 mg/kg IVE + 1,000 mg/kg PGO (IVE+PGO+). All the piglets were housed individually for the 42-d trial period after 7-d adaptation. The piglets were euthanized at the end of the experiment and the liver and jejunum samples were taken and subjected to immunohistochemistry, Western blotting, as well as antioxidant and qRT-PCR analysis. Significant interactions were observed between IVE and PGO for total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) in serum (42 d), liver, and jejunum; malondialdehyde (MDA) in serum (21 d); and mRNA and protein expression of kelch sample related protein-1 (Keap1) and nuclear factor erythroid-2 related factor (Nrf2)/Keap1 in the liver and jejunum (P < 0.05). Both IVE and PGO improved (P < 0.05) T-SOD and GSH-Px in the serum (42 d), liver, and jejunum, and the mRNA and protein expression of Nrf2 and Nrf2/Keap1 in the liver and jejunum, but decreased (P < 0.05) MDA in the serum (21 d) and the mRNA and protein expression of Keap1 in the liver and jejunum. Immunohistochemical results confirmed that IVE and PGO enhanced the positive reactions of Nrf2 but weakened Keap1 in both the liver and jejunum. In conclusion, the results confirmed that IVE (500 mg/kg) and PGO (1,000 mg/kg) can improve the antioxidant capacity of weaned piglets and that the interaction effect between IVE and PGO is significant. At the same time, the fact that IVE and PGO activate the Nrf2/Keap1 in the liver and jejunum signaling pathway suggests that they play an important role in the ameliorative antioxidant capacity of weaned piglets. Therefore, the combination of IVE and PGO could be recommended as a new potential alternative to antibiotics in piglets' diets. [ABSTRACT FROM AUTHOR]

Inspired by fast and selective water transport in natural Aquaporin (AQP), imidazole-quartet water channels (IQWC) constructed by imidazole-ureido (IU) molecular scaffolds, are excellent candidates to design membranes with fast and selective water transport properties. In this work, a water binding IU bola-amphiphile-imidazole compound was embedded with the hydrophilic and laminated graphene oxide (GO) to prepare IU-GO membrane. H NMR, TG, XRD, SEM, AFM and FTIR were conducted to characterize physicochemical properties of IU and IU-GO membrane. It was demonstrated that IU could well interact with GO to generate a robust membrane structure. The resulting IU-GO membranes were applied for pervaporation dehydration of n -butanol-water. The total flux and the separation factor of the optimized IU-GO membrane were 3506 g·m-2·h-1 and 4454 respectively when the membrane was measured at 343 K with butanol content of 80 wt%. Furthermore, the membrane exhibited good stability and separation performance during 120 h continuous operation test, showing great potential in application of solvent dehydration. • Imidazole-ureido (IU) was embedded into graphene oxide (GO) laminate. • IU-GO membrane was applied for n -butanol dehydration via pervaporation. • The membrane exhibited high flux and separation factor for n -butanol dehydration. [ABSTRACT FROM AUTHOR]

Background: Co-infection with avian leukosis virus subgroup J and reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown. Results: In this study, exosomal RNAs from CEF cells infected with ALV-J, REV or both at the optimal synergistic infection time were analysed by Illumina RNA deep sequencing. A total of 54 (23 upregulated and 31 downregulated) and 16 (7 upregulated and 9 downregulated) miRNAs were identified by comparing co-infection with two viruses, single-infected ALV-J and REV, respectively. Moreover, five key miRNAs, including miR-184-3p, miR-146a-3p, miR-146a-5p, miR-3538 and miR-155, were validated in both exosomes and CEF cells by qRT-PCR. GO annotation and KEGG pathway analysis of the miRNA target genes showed that the five differentially expressed miRNAs participated in virus-vector interaction, oxidative phosphorylation, energy metabolism and cell growth. Conclusions: We demonstrated that REV and ALV-J synergistically increased the accumulation of exosomal miRNAs, which sheds light on the synergistic molecular mechanism of ALV-J and REV. [ABSTRACT FROM AUTHOR]