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In this study, three-factor and three-level orthogonal experiment was used to optimize the detection system of clinical renal function marker β2-microglobulin latex enhanced immune turbidity, so as to prepare a detection system with a wider linear range, solve the false negative problem in clinical detection of high-concentration samples, and improve the detection accuracy. Based on latex enhanced immunoturbidimetry, automatic biochemical analyzer was used to establish the linear relationship between the β2-microglobulin standard with different concentrations and the absorbance change value of 546 nm, and optimize the antibody source, latex microsphere particle size, activator concentration, sealer concentration and buffer system in the β2-microglobulin detection system. The linear range and accuracy of the optimized detection system were evaluated. After optimization, the optimal detection system was obtained, and its detection linear range was 0.2-30 mg/L, which reached the upper limit of the reference range of β2-microglobulin in normal population by 10 times, and the accuracy was in line with clinical standards, which solved the false negative problem of high-concentration samples in clinical testing, eliminated the repeated dilution process of high-concentration samples, simplified the detection steps, and improved the detection accuracy.

Layered vanadium-based materials are considered to be great potential electrode materials for aqueous Zn-ion batteries (AZIBs). The improvement of the electrochemical properties of vanadium-based materials is a hot research topic but still a challenge. Herein, a composite of Zn-ion pre-intercalated V2O5·nH2O combined with reduced graphene oxide (ZnVOH/rGO) is synthesized by a facile hydrothermal method and it shows improved Zn-ion storage. ZnVOH/rGO delivers a capacity of 325 mAh·g−1 at 0.1 A·g−1, and this value can still reach 210 mAh·g−1 after 100 cycles. Additionally, it exhibits 196 mAh·g−1 and keeps 161 mAh·g−1 after 1200 cycles at 4 A·g−1. The achieved performances are much higher than that of ZnVOH and VOH. All results reveal that Zn2+ as "pillars" expands the interlayer distance of VOH and facilitates the fast kinetics, and rGO improves the electron flow. They both stabilize the structure and enhance efficient Zn2+ migration. All findings demonstrate ZnVOH/rGO's potential as a perspective cathode material for AZIBs. [ABSTRACT FROM AUTHOR]

CoFe-based catalysts are efficient electrocatalysts for the oxygen evolution reaction (OER) in alkaline media. Here, we present a simple one-pot hydrothermal method for synthesizing a series of CoFe glycerates with controllable surface morphologies and investigate their potential as highly efficient catalysts for the OER in alkaline media. These CoFe glycerates exhibit a unique yolk-shell microsphere structure assembled from ultrathin nanosheets. The adjustment of the surface nanosheet size is achieved by varying the CoFe ratio, ensuring a more efficient electrocatalytic system for the OER process. Due to the abundant active sites provided by the yolk-shell structure and interleaved ultrathin nanosheets, Co 3 Fe 1 glycerate (Co 3 Fe 1 gly) demonstrates a low overpotential (283 mV) and a small Tafel slope (44.61 mV dec -1 ) at 10 mA cm -2 . Additionally, Co 3 Fe 1 gly exhibits excellent durability in alkaline electrolytes. Moreover, a series of characterizations demonstrate that the active sites of Co 3 Fe 1 gly are the high-valence Co species generated during the OER process. This study opens a promising avenue for utilizing efficient and low-cost electrocatalysts to enhance OER performance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Objective: To investigate the role of histone acetylation in regulating influenza virus replicative intermediate double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells., Methods: A549 cells were treated with influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6 mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively., Results: Influenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6 expression., Conclusions: Influenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by histone acetylation.