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3. Lenvatinib Plus Pembrolizumab versus Doxorubicin for Advanced or Recurrent Endometrial Cancer with Short Treatment-Free Intervals Following First-Line Carboplatin Plus Paclitaxel.

Author

Wang, Shao-Jing, Chen, Hsin-Hua, Sun, Lou, Shih, Yu-Hsiang, Lu, Ting-Fang, Chen, Yen-Fu, Fan, Chun-Ting, Hsu, Shih-Tien, Liu, Chin-Ku, Hwang, Sheau-Feng, and Lu, Chien-Hsing

Subjects
PROPENSITY score matching, ELECTRONIC health records, DOXORUBICIN, ENDOMETRIAL cancer, PEMBROLIZUMAB
Abstract

Background: The treatment-free interval is a significant predictor of worse prognosis and poor response rates of the second-line treatment in patients with carboplatin and paclitaxel (PT)-pretreated, advanced, or recurrent endometrial cancer (EC). Whether lenvatinib plus pembrolizumab still confers a survival benefit compared with doxorubicin in patients with platinum-free intervals of <6 months remains unclear. Methods: This multi-institutional retrospective analysis was performed using de-identified electronic health records from the TriNetX Research Network. Patients with advanced or recurrent ECs who received lenvatinib plus pembrolizumab or doxorubicin within six months of first-line PT were identified. A 1:1 propensity score matching (PSM) was conducted to control for potential confounding variables. Overall survival (OS) and adverse event profile were the primary and secondary outcomes. Results: Between January 2018 and February 2024, 130 patients with PT-treated, advanced, or recurrent ECs who received lenvatinib plus pembrolizumab and 122 patients who received doxorubicin at a platinum-free interval of <6 months were identified across 31 healthcare organizations. In the balanced cohort following PSM with 117 patients in each group, treatment with lenvatinib plus pembrolizumab was associated with improved OS compared with treatment with doxorubicin (12.8 vs. 8.2 months, p = 0.012, hazard ratio: 0.65, 95% confidence interval: 0.46–0.91). Regarding adverse event analysis, a higher incidence of hypothyroidism and proteinuria was observed with lenvatinib plus pembrolizumab, and more hematological toxicities were observed with doxorubicin. Conclusions: in patients with treatment-free intervals of <6 months, lenvatinib plus pembrolizumab still confers improved survival compared with doxorubicin in PT-treated, advanced, or recurrent ECs. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Evaluation of a novel vaginal cells self‐sampling device for human papillomavirus testing in cervical cancer screening: A clinical trial assessing reliability and acceptability

Author

Yang, Chung‐Yao, primary, Chang, Ting‐Chang, additional, Chou, Hung‐Hsueh, additional, Chao, Angel, additional, Hsu, Shih‐Tien, additional, Shih, Yu‐Hsiang, additional, Huang, Huei‐Jean, additional, Lin, Cheng‐Tao, additional, Chen, Min‐Yu, additional, Sun, Lou, additional, Huang, Kuan‐Gen, additional, Wu, Kai‐Yun, additional, Hsieh, Wu‐Chiao, additional, Huang, Yi‐Ting, additional, Chen, Liang‐Hsuan, additional, Lu, Chien‐Hsing, additional, Lin, Hao, additional, and Cheng, Chao‐Min, additional

Published
2024
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12. Can HPV Test on Random Urine Replace Self-HPV Test on Vaginal Self-Samples or Clinician-Collected Cervical Samples?

Author

Shih,Yu-Hsiang, Sun,Lou, Hsu,Shih-Tien, Chen,Ming-Jer, Lu,Chien-Hsing, Shih,Yu-Hsiang, Sun,Lou, Hsu,Shih-Tien, Chen,Ming-Jer, and Lu,Chien-Hsing

Abstract

Yu-Hsiang Shih, Lou Sun, Shih-Tien Hsu, Ming-Jer Chen, Chien-Hsing Lu Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, Taichung, TaiwanCorrespondence: Chien-Hsing Lu, Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, 1650 Taiwan Boulevard Sect. 4, Taichung, Taiwan, Tel +886-4-23592525, Email chlu@vghtc.gov.twObjective: This study investigated whether random urine (RU) samples can be used to accurately identify human papillomavirus (HPV) and whether these samples can replace self-collected vaginal samples in HPV tests.Methods: A total of 167 patients with abnormal Pap smears were recruited. The patients provided self-collected vaginal and RU samples for HPV testing. Clinicians obtained cervical samples from the patients. Colposcopy examination and cervical biopsy were performed. Hybrid Capture II (HC II) and Cervista tests were used to detect HPV in the RU samples.Results: The results of tests on clinician-collected cervical samples were used as the benchmark. The sensitivities of the Cervista tests on vaginal samples and the HC II and Cervista tests on RU samples were 75.00%, 49.07%, and 44.44%, respectively. After we adjusted the HPV detection cutoff value for urine samples based on values in the receiver operating characteristic curve, the sensitivities of the HC II and Cervista tests increased to 63.89% and 58.33%, respectively. In 167 patients, 59 had cervix biopsies showing CIN2 or worse (CIN2+). For CIN2+, the sensitivity was 47.5% and 50.8% in the HC II and Cervista tests on RU samples, respectively.Conclusion: HPV tests on RU samples had approximately 60% sensitivity to HPV tests on clinician-collected cervical samples after the cutoff values were adjusted. For CIN2+, the sensitivity was only approximately 50%. Further studies and improvements in urine-based HPV testing are needed to establish it as a more convenient and accessible method for detecting HPV and cervical dysplasia in patients.Keywords: HPV

Published
2023

13. Women with endometriosis have higher comorbidities: Analysis of domestic data in Taiwan

Author

Chang, Yen-Hou, Chang, Yi, Chao, Kuan-Chong, Chen, Yi-Jen, Chuang, Chi-Mu, Huang, Chen-Yu, Jiang, Ling-Yu, Li, Hsin-Yang, Liu, Chia-Hao, Sun, Pi-Lin, Wen, Kuo-Chang, Wu, Hua-Hsi, Yu, Hann-Chin, Ju, Fong-Yuan, Tsai, Chih-Ping, Chang, Wen-Hsun, Hsu, Yen-Mei, Huang, Shu-Yun, Lee, Na-Rong, Chen, Chih-Yao, Chang, Ting-Chen, Chang, Wen-Chun, Chen, Chii-Hou, Chen, Ruey-Jian, Chow, Song-Nan, Lien, Yih-Ron, Sheu, Bor-Ching, Torng, Pao-Ling, Yen, Men-Luh, Lee, Wen-Ling, Wang, Kuan-Chin, Chang, Chih-Long, Chen, Chih-Ping, Chen, Jen-Ruei, Chen, Tze-Chien, Huang, Jian-Pei, Huang, Ming-Chao, Wang, Yeou-Lih, Chang, Cheng-Chang, Liu, Jah-Yao, Su, Her-Young, Wang, Yu-Chi, Yu, Mu-Hsien, Chu, Ching-Chuang, Huang, Lee-Wen, Seow, Kok-Min, Lai, Tsung-Hsuan, Lee, Fa-Kung, Chen, Ching-Hui, Liu, Wei-Min, Chiou, Jyh-Shin, Huang, Ben-Shian, Lu, Yen-Feng, Hsiao, Sheng-Mou, Sun, Hsu-Dong, Wu, Wen-Yih, Chen, Kuo-Hu, Hung, Jeng-Hsiu, Lai, Hung-Cheng, Yuan, Chiou-Chung, Hsieh, Ching-Hung, Wang, Chin-Jung, Chan, Chia-Hao, Chang, Shing-Jyh, Shih, Chuan-Chi, Hung, Man-Jung, Hsu, Shih-Tien, Ke, Yu-Min, Lu, Chien-Hsing, Sun, Lou, Chang, Wei-Chun, Hung, Yao-Ching, Lin, Wu-Chou, Wang, Po-Hui, Chen, Tze-Ho, Li, Yiu-Tai, Wu, Meng-Hsing, Huang, Kuo-Feng, Chuang, Fei-Chi, Fu, Hung-Chun, Kung, Fu-Tsai, Huang, Kuan-Hui, Chen, San-Nung, Chiang, An-Jen, Li, Ju-Yueh, Lin, Li-Te, Tsai, Hsiao-Wen, Tsui, Kuan-Hao, Teng, Sen-Wen, Horng, Huann-Cheng, Ho, Chi-Hong, Yen, Ming-Shyen, Chao, Hsiang-Tai, and Wang, Peng-Hui

Published
2016
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14. Uterine sarcoma part III—Targeted therapy: The Taiwan Association of Gynecology (TAG) systematic review

Author

Chang, Yen-Hou, Chang, Yi, Chao, Hsiang-Tai, Chao, Kuan-Chong, Chuang, Chi-Mu, Ho, Chi-Hong, Horng, Huann-Cheng, Huang, Chen-Yu, Jiang, Ling-Yu, Liu, Chia-Hao, Li, Hsin-Yang, Sun, Pi-Lin, Wu, Hua-Hsi, Ju, Fong-Yuan, Tsai, Chih-Ping, Chang, Wen-Hsun, Hsu, Yen-Mei, Huang, Shu-Yun, Lee, Na-Rong, Chen, Chih-Yao, Chang, Wen-Chun, Chen, Chii-Hou, Chen, Ruey-Jian, Chow, Song-Nan, Lien, Yih-Ron, Sheu, Bor-Ching, Torng, Pao-Ling, Wei, Lin-Hung, Yen, Men-Luh, Lee, Wen-Ling, Wang, Kuan-Chin, Chang, Chih-Long, Chen, Chih-Ping, Chen, Tze-Chien, Huang, Jian-Pei, Huang, Ming-Chao, Wang, Yeou-Lih, Chang, Cheng-Chang, Liu, Jah-Yao, Su, Her-Young, Wang, Yu-Chi, Yu, Mu-Hsien, Chu, Ching-Chuang, Huang, Lee-Wen, Seow, Kok-Min, Lai, Tsung-Hsuan, Lee, Fa-Kung, Chen, Ching-Hui, Liu, Wei-Min, Chiou, Jyh-Shin, Huang, Ben-Shian, Lu, Yen-Feng, Hsiao, Sheng-Mou, Sun, Hsu-Dong, Wu, Wen-Yih, Teng, Sen-Wen, Chen, Kuo-Hu, Hung, Jeng-Hsiu, Lai, Hung-Cheng, Yuan, Chiou-Chung, Hsieh, Ching-Hung, Wang, Chin-Jung, Chan, Chia-Hao, Chang, Shing-Jyh, Shih, Chuan-Chi, Hung, Man-Jung, Hsu, Shih-Tien, Ke, Yu-Min, Lu, Chien-Hsing, Sun, Lou, Chang, Wei-Chun, Hung, Yao-Ching, Lin, Wu-Chou, Wang, Po-Hui, Chen, Tze-Ho, Wu, Meng-Hsing, Li, Yiu-Tai, Huang, Kuo-Feng, Chuang, Fei-Chi, Fu, Hung-Chun, Kung, Fu-Tsai, Huang, Kuan-Hui, Chen, San-Nung, Chiang, An-Jen, Li, Ju-Yueh, Liou, Wen-Shiung, Lin, Li-Te, Tsai, Hsiao-Wen, Tsui, Kuan-Hao, Yen, Ming-Shyen, Chen, Jen-Ruei, Wang, Peng-Hui, Wen, Kuo-Chang, Chen, Yi-Jen, and Ng, Heung-Tat

Published
2016
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16. Uterine sarcoma Part II—Uterine endometrial stromal sarcoma: The TAG systematic review

Author

Chang, Yen-Hou, Chang, Yi, Chao, Hsiang-Tai, Chao, Kuan-Chong, Chuang, Chi-Mu, Ho, Chi-Hong, Huang, Chen-Yu, Hung, Zhi-Chen, Jiang, Ling-Yu, Lau, Hei-Yu, Li, Hsin-Yang, Lin, Chi-Yao, Liu, Chia-Hao, Sun, Pi-Lin, Twu, Nae-Fang, Wu, Hua-Hsi, Yu, Hann-Chin, Ju, Fong-Yuan, Tsai, Chih-Ping, Chang, Wen-Hsun, Hsu, Yen-Mei, Lee, Na-Rong, Chen, Chih-Yao, Chang, Ting-Chen, Chang, Wen-Chun, Chen, Chii-Hou, Chen, Ruey-Jian, Chow, Song-Nan, Lien, Yih-Ron, Sheu, Bor-Ching, Torng, Pao-Ling, Yen, Men-Luh, Lee, Wen-Ling, Wang, Kuan-Chin, Chang, Chih-Long, Chen, Chih-Ping, Chen, Jen-Ruei, Chen, Tze-Chien, Huang, Jian-Pei, Huang, Ming-Chao, Wang, Yeou-Lih, Chang, Cheng-Chang, Liu, Jah-Yao, Su, Her-Young, Wang, Yu-Chi, Yu, Mu-Hsien, Huang, Lee-Wen, Seow, Kok-Min, Lai, Tsung-Hsuan, Lee, Fa-Kung, Chen, Ching-Hui, Huang, Ben-Shian, Hsiao, Sheng-Mou, Sun, Hsu-Dong, Wu, Wen-Yih, Teng, Sen-Wen, Chen, Kuo-Hu, Hung, Jeng-Hsiu, Lai, Hung-Cheng, Yuan, Chiou-Chung, Hsieh, Ching-Hung, Wang, Chin-Jung, Chang, Shing-Jyh, Hung, Man-Jung, Hsu, Shih-Tien, Ke, Yu-Min, Lu, Chien-Hsing, Sun, Lou, Chang, Wei-Chun, Hung, Yao-Ching, Lin, Wu-Chou, Wang, Po-Hui, Chen, Tze-Ho, Li, Yiu-Tai, Wu, Meng-Hsing, Huang, Kuo-Feng, Fu, Hung-Chun, Chen, San-Nung, Chiang, An-Jen, Li, Ju-Yueh, Lin, Li-Te, Tsai, Hsiao-Wen, Tsui, Kuan-Hao, Horng, Huann-Cheng, Wen, Kuo-Chang, Wang, Peng-Hui, Chen, Yi-Jen, Yen, Ming-Shyen, and Ng, Heung-Tat

Published
2016
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17. Uterine sarcoma Part I—Uterine leiomyosarcoma: The Topic Advisory Group systematic review

Author

Chang, Yen-Hou, Chang, Yi, Chao, Hsiang-Tai, Chao, Kuan-Chong, Chuang, Chi-Mu, Ho, Chi-Hong, Huang, Chen-Yu, Hung, Zhi-Chen, Jiang, Ling-Yu, Lau, Hei-Yu, Li, Hsin-Yang, Lin, Chi-Yao, Liu, Chia-Hao, Sun, Pi-Lin, Twu, Nae-Fang, Wu, Hua-Hsi, Yu, Hann-Chin, Ju, Fong-Yuan, Tsai, Chih-Ping, Chang, Wen-Hsun, Hsu, Yen-Mei, Lee, Na-Rong, Chen, Chih-Yao, Chang, Ting-Chen, Chang, Wen-Chun, Chen, Chii-Hou, Chen, Ruey-Jian, Chow, Song-Nan, Lien, Yih-Ron, Sheu, Bor-Ching, Torng, Pao-Ling, Yen, Men-Luh, Lee, Wen-Ling, Wang, Kuan-Chin, Chang, Chih-Long, Chen, Chih-Ping, Chen, Jen-Ruei, Chen, Tze-Chien, Huang, Jian-Pei, Huang, Ming-Chao, Wang, Yeou-Lih, Chang, Cheng-Chang, Liu, Jah-Yao, Su, Her-Young, Wang, Yu-Chi, Yu, Mu-Hsien, Huang, Lee-Wen, Seow, Kok-Min, Lai, Tsung-Hsuan, Lee, Fa-Kung, Chen, Ching-Hui, Huang, Ben-Shian, Hsiao, Sheng-Mou, Sun, Hsu-Dong, Wu, Wen-Yih, Teng, Sen-Wen, Chen, Kuo-Hu, Hung, Jeng-Hsiu, Lai, Hung-Cheng, Yuan, Chiou-Chung, Hsieh, Ching-Hung, Wang, Chin-Jung, Chang, Shing-Jyh, Hung, Man-Jung, Hsu, Shih-Tien, Ke, Yu-Min, Lu, Chien-Hsing, Sun, Lou, Chang, Wei-Chun, Hung, Yao-Ching, Lin, Wu-Chou, Wang, Po-Hui, Chen, Tze-Ho, Li, Yiu-Tai, Wu, Meng-Hsing, Huang, Kuo-Feng, Fu, Hung-Chun, Chen, San-Nung, Chiang, An-Jen, Li, Ju-Yueh, Lin, Li-Te, Tsai, Hsiao-Wen, Tsui, Kuan-Hao, Wen, Kuo-Chang, Horng, Huann-Cheng, Wang, Peng-Hui, Chen, Yi-Jen, Yen, Ming-Shyen, and Ng, Heung-Tat

Published
2016
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19. Can HPV Test on Random Urine Replace Self-HPV Test on Vaginal Self-Samples or Clinician-Collected Cervical Samples?

Author

Shih, Yu-Hsiang, Sun, Lou, Hsu, Shih-Tien, Chen, Ming-Jer, and Lu, Chien-Hsing

Subjects
HUMAN papillomavirus, RECEIVER operating characteristic curves, URINALYSIS, CERVICAL intraepithelial neoplasia, PAP test
Abstract

This study investigated whether random urine (RU) samples can be used to accurately identify human papillomavirus (HPV) and whether these samples can replace self-collected vaginal samples in HPV tests.Methods: A total of 167 patients with abnormal Pap smears were recruited. The patients provided self-collected vaginal and RU samples for HPV testing. Clinicians obtained cervical samples from the patients. Colposcopy examination and cervical biopsy were performed. Hybrid Capture II (HC II) and Cervista tests were used to detect HPV in the RU samples.Results: The results of tests on clinician-collected cervical samples were used as the benchmark. The sensitivities of the Cervista tests on vaginal samples and the HC II and Cervista tests on RU samples were 75.00%, 49.07%, and 44.44%, respectively. After we adjusted the HPV detection cutoff value for urine samples based on values in the receiver operating characteristic curve, the sensitivities of the HC II and Cervista tests increased to 63.89% and 58.33%, respectively. In 167 patients, 59 had cervix biopsies showing CIN2 or worse (CIN2+). For CIN2+, the sensitivity was 47.5% and 50.8% in the HC II and Cervista tests on RU samples, respectively.Conclusion: HPV tests on RU samples had approximately 60% sensitivity to HPV tests on clinician-collected cervical samples after the cutoff values were adjusted. For CIN2+, the sensitivity was only approximately 50%. Further studies and improvements in urine-based HPV testing are needed to establish it as a more convenient and accessible method for detecting HPV and cervical dysplasia in patients. [ABSTRACT FROM AUTHOR]

Published
2023
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24. Endometrial Cancer Detection Using a Cervical DNA Methylation Assay (MPap) in Women with Abnormal Uterine Bleeding: A Multicenter Hospital-Based Validation Study

Author

Wen, Kuo-Chang, primary, Huang, Rui-Lan, additional, Chen, Lin-Yu, additional, Wu, Tzu-I, additional, Lu, Chien-Hsing, additional, Chu, Tang-Yuan, additional, Ou, Yu-Che, additional, Wu, Chen-Hsuan, additional, Hsu, Shih-Tien, additional, Ding, Dah-Ching, additional, Chu, Ling-Hui, additional, Chen, Chien-Wen, additional, Chang, Heng-Cheng, additional, Liu, Yu-Shu, additional, Wang, Hui-Chen, additional, Weng, Yu-Chun, additional, Su, Po-Hsuan, additional, Lin, Hao, additional, and Lai, Hung-Cheng, additional

Published
2022
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36. Transient characteristics of universal cells on human‐induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources

Author

Sung, Tzu‐Cheng, primary, Jiang, Yi‐Peng, additional, Hsu, Jhe‐Yu, additional, Ling, Qing‐Dong, additional, Chen, Hao, additional, Kumar, Suresh S., additional, Chang, Yung, additional, Hsu, Shih‐Tien, additional, Ye, Qingsong, additional, and Higuchi, Akon, additional

Published
2021
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38. Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels

Author

Chen, Li Hua, Sung, Tzu Cheng, Lee, Henry Hsin Chung, Higuchi, Akon, Su, Huan Chiao, Lin, Kuan Ju, Huang, Yu Ru, Ling, Qing Dong, Kumar, Suresh S., Alarfaj, Abdullah A., Murugan, Alwarkurichi Munusamy, Nasu, Michiyo, Chen, Da Chung, Hsu, Shih Tien, Chang, Yung, Lee, Kuei Fang, Wang, Han Chow, Umezawa, Akihiro, Chen, Li Hua, Sung, Tzu Cheng, Lee, Henry Hsin Chung, Higuchi, Akon, Su, Huan Chiao, Lin, Kuan Ju, Huang, Yu Ru, Ling, Qing Dong, Kumar, Suresh S., Alarfaj, Abdullah A., Murugan, Alwarkurichi Munusamy, Nasu, Michiyo, Chen, Da Chung, Hsu, Shih Tien, Chang, Yung, Lee, Kuei Fang, Wang, Han Chow, and Umezawa, Akihiro

Abstract

Recombinant vitronectin-grafted hydrogels were developed by adjusting surface charge of the hydrogels with grafting of poly-l-lysine for optimal culture of human embryonic stem cells (hESCs) under xeno- and feeder-free culture conditions, with elasticity regulated by crosslinking time (10–30 kPa), in contrast to conventional recombinant vitronectin coating dishes, which have a fixed stiff surface (3 GPa). hESCs proliferated on the hydrogels for over 10 passages and differentiated into the cells derived from three germ layers indicating the maintenance of pluripotency. hESCs on the hydrogels differentiated into cardiomyocytes under xeno-free culture conditions with much higher efficiency (80% of cTnT⁺ cells) than those on conventional recombinant vitronectin or Matrigel-coating dishes just only after 12 days of induction. It is important to have an optimal design of cell culture biomaterials where biological cues (recombinant vitronectin) and physical cues (optimal elasticity) are combined for high differentiation of hESCs into specific cell lineages, such as cardiomyocytes, under xeno-free and feeder-free culture conditions.

Published
2019

39. Proliferation and osteogenic differentiation of amniotic fluid-derived stem cells

Author

Muduli, Saradaprasan, Lee, Henry Hsin-Chung, Yang, Jia-Sin, Chen, Ting-Yeh, Higuchi, Akon, Subbiah, Suresh Kumar, Alarfaj, Abdullah A., Murugan, A. Munusamy, Benelli, Giovanni, Murugan, Kadarkarai, Liu, Ching-Yuan, Chen, Yi-Fan, Chang, Yung, Moorthy, Brintha, Wang, Han-Chow, Hsu, Shih-Tien, Ling, Qing-Dong, Muduli, Saradaprasan, Lee, Henry Hsin-Chung, Yang, Jia-Sin, Chen, Ting-Yeh, Higuchi, Akon, Subbiah, Suresh Kumar, Alarfaj, Abdullah A., Murugan, A. Munusamy, Benelli, Giovanni, Murugan, Kadarkarai, Liu, Ching-Yuan, Chen, Yi-Fan, Chang, Yung, Moorthy, Brintha, Wang, Han-Chow, Hsu, Shih-Tien, and Ling, Qing-Dong

Abstract

Human amniotic fluid-derived stem cells (hAFCs) are pluripotent fetal cells capable of differentiating into multiple lineages, including cell types of each of the three embryonic germ layers. Proper differentiation and maintenance of pluripotency, the defining characteristics of stem cells, are regulated not only by the cells themselves but also by their microenvironment. Furthermore, the physical characteristics of the cell culture materials, such as material elasticity, influence the results of stem cell differentiation. We investigated the osteogenic differentiation efficiency of hAFCs cultured on cell culture materials with different elasticities that were grafted with extracellular matrix-derived oligopeptides. Polyvinyl alcohol-co-itaconic acid (PV) hydrogels with different elasticities were prepared by controlling the crosslinking intensity, and the resulting PV hydrogels were grafted with and without extracellular matrix (ECM)-derived oligopeptides. Specific ECM-derived oligopeptides were used to maintain the pluripotency of AFCs and were determined by evaluation of pluripotent gene expression (Sox2 and Oct4). The osteogenic differentiation efficiency of the hAFCs, cultured on PV hydrogels grafted with and without ECM-derived oligopeptides, was analyzed by alkaline phosphatase activity, Alizarin Red S staining, and von Kossa staining. Unmodified PV hydrogels induced osteoblast differentiation of hAFCs with high efficiency. We conclude that the hAFCs interacting with ECM-derived oligopeptides tended to maintain an undifferentiated state.

Published
2017

40. Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells

Author

Higuchi, Akon, Subbiah, Suresh Kumar, Ling, Qing-Dong, A. Alarfaj, Abdullah, Munusamy, Murugan A., Murugan, Kadarkarai, Hsu, Shih-Tien, Benelli, Giovanni, Umezawa, Akihiro, Higuchi, Akon, Subbiah, Suresh Kumar, Ling, Qing-Dong, A. Alarfaj, Abdullah, Munusamy, Murugan A., Murugan, Kadarkarai, Hsu, Shih-Tien, Benelli, Giovanni, and Umezawa, Akihiro

Abstract

Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment of Alzheimer's disease and diabetes, respectively. However, it is challenging to guide hPSC differentiation into desired cell lineages due to their varying differentiation ability. A reasonable strategy is to mimic the stem cell microenvironment for the differentiation of hPSCs into specific cell lineages using optimal polymeric biomaterials for hPSC culture. This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages. The recent trend in protocols avoids embryoid body (EB, aggregated cells) formation because EBs contain different types of cells. The combination of appropriate differentiation protocols and cell culture polymeric biomaterials for the differentiation of hPSCs into specific cell lineages will produce a large quantity of highly pure GMP-grade differentiated cells for use in translational medicine.

Published
2017

41. Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Author

Muduli, Saradaprasan, Chen, Li-Hua, Li, Meng-Pei, Heish, Zhao-wen, Liu, Cheng-Hui, Kumar, Suresh, Alarfaj, Abdullah A., Munusamy, Murugan A., Benelli, Giovanni, Murugan, Kadarkarai, Wang, Han-Chow, Chen, Da-Chung, Hsu, Shih-Tien, Chang, Shih-Chang, Higuchi, Akon, Muduli, Saradaprasan, Chen, Li-Hua, Li, Meng-Pei, Heish, Zhao-wen, Liu, Cheng-Hui, Kumar, Suresh, Alarfaj, Abdullah A., Munusamy, Murugan A., Benelli, Giovanni, Murugan, Kadarkarai, Wang, Han-Chow, Chen, Da-Chung, Hsu, Shih-Tien, Chang, Shih-Chang, and Higuchi, Akon

Abstract

The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

Published
2017

44. Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Author

Lin, Hong Reng, primary, Heish, Chao-Wen, additional, Liu, Cheng-Hui, additional, Muduli, Saradaprasan, additional, Li, Hsing-Fen, additional, Higuchi, Akon, additional, Kumar, S. Suresh, additional, Alarfaj, Abdullah A., additional, Munusamy, Murugan A., additional, Hsu, Shih-Tien, additional, Chen, Da-Chung, additional, Benelli, Giovanni, additional, Murugan, Kadarkarai, additional, Cheng, Nai-Chen, additional, Wang, Han-Chow, additional, and Wu, Gwo-Jang, additional

Published
2017
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46. Women with endometriosis have higher comorbidities: Analysis of domestic data in Taiwan

Author

Teng, Sen-Wen, primary, Horng, Huann-Cheng, additional, Ho, Chi-Hong, additional, Yen, Ming-Shyen, additional, Chao, Hsiang-Tai, additional, Wang, Peng-Hui, additional, Chang, Yen-Hou, additional, Chang, Yi, additional, Chao, Kuan-Chong, additional, Chen, Yi-Jen, additional, Chuang, Chi-Mu, additional, Huang, Chen-Yu, additional, Jiang, Ling-Yu, additional, Li, Hsin-Yang, additional, Liu, Chia-Hao, additional, Sun, Pi-Lin, additional, Wen, Kuo-Chang, additional, Wu, Hua-Hsi, additional, Yu, Hann-Chin, additional, Ju, Fong-Yuan, additional, Tsai, Chih-Ping, additional, Chang, Wen-Hsun, additional, Hsu, Yen-Mei, additional, Huang, Shu-Yun, additional, Lee, Na-Rong, additional, Chen, Chih-Yao, additional, Chang, Ting-Chen, additional, Chang, Wen-Chun, additional, Chen, Chii-Hou, additional, Chen, Ruey-Jian, additional, Chow, Song-Nan, additional, Lien, Yih-Ron, additional, Sheu, Bor-Ching, additional, Torng, Pao-Ling, additional, Yen, Men-Luh, additional, Lee, Wen-Ling, additional, Wang, Kuan-Chin, additional, Chang, Chih-Long, additional, Chen, Chih-Ping, additional, Chen, Jen-Ruei, additional, Chen, Tze-Chien, additional, Huang, Jian-Pei, additional, Huang, Ming-Chao, additional, Wang, Yeou-Lih, additional, Chang, Cheng-Chang, additional, Liu, Jah-Yao, additional, Su, Her-Young, additional, Wang, Yu-Chi, additional, Yu, Mu-Hsien, additional, Chu, Ching-Chuang, additional, Huang, Lee-Wen, additional, Seow, Kok-Min, additional, Lai, Tsung-Hsuan, additional, Lee, Fa-Kung, additional, Chen, Ching-Hui, additional, Liu, Wei-Min, additional, Chiou, Jyh-Shin, additional, Huang, Ben-Shian, additional, Lu, Yen-Feng, additional, Hsiao, Sheng-Mou, additional, Sun, Hsu-Dong, additional, Wu, Wen-Yih, additional, Chen, Kuo-Hu, additional, Hung, Jeng-Hsiu, additional, Lai, Hung-Cheng, additional, Yuan, Chiou-Chung, additional, Hsieh, Ching-Hung, additional, Wang, Chin-Jung, additional, Chan, Chia-Hao, additional, Chang, Shing-Jyh, additional, Shih, Chuan-Chi, additional, Hung, Man-Jung, additional, Hsu, Shih-Tien, additional, Ke, Yu-Min, additional, Lu, Chien-Hsing, additional, Sun, Lou, additional, Chang, Wei-Chun, additional, Hung, Yao-Ching, additional, Lin, Wu-Chou, additional, Wang, Po-Hui, additional, Chen, Tze-Ho, additional, Li, Yiu-Tai, additional, Wu, Meng-Hsing, additional, Huang, Kuo-Feng, additional, Chuang, Fei-Chi, additional, Fu, Hung-Chun, additional, Kung, Fu-Tsai, additional, Huang, Kuan-Hui, additional, Chen, San-Nung, additional, Chiang, An-Jen, additional, Li, Ju-Yueh, additional, Lin, Li-Te, additional, Tsai, Hsiao-Wen, additional, and Tsui, Kuan-Hao, additional

Published
2016
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47. Uterine sarcoma part III—Targeted therapy: The Taiwan Association of Gynecology (TAG) systematic review

Author

Yen, Ming-Shyen, primary, Chen, Jen-Ruei, additional, Wang, Peng-Hui, additional, Wen, Kuo-Chang, additional, Chen, Yi-Jen, additional, Ng, Heung-Tat, additional, Chang, Yen-Hou, additional, Chang, Yi, additional, Chao, Hsiang-Tai, additional, Chao, Kuan-Chong, additional, Chuang, Chi-Mu, additional, Ho, Chi-Hong, additional, Horng, Huann-Cheng, additional, Huang, Chen-Yu, additional, Jiang, Ling-Yu, additional, Liu, Chia-Hao, additional, Li, Hsin-Yang, additional, Sun, Pi-Lin, additional, Wu, Hua-Hsi, additional, Ju, Fong-Yuan, additional, Tsai, Chih-Ping, additional, Chang, Wen-Hsun, additional, Hsu, Yen-Mei, additional, Huang, Shu-Yun, additional, Lee, Na-Rong, additional, Chen, Chih-Yao, additional, Chang, Wen-Chun, additional, Chen, Chii-Hou, additional, Chen, Ruey-Jian, additional, Chow, Song-Nan, additional, Lien, Yih-Ron, additional, Sheu, Bor-Ching, additional, Torng, Pao-Ling, additional, Wei, Lin-Hung, additional, Yen, Men-Luh, additional, Lee, Wen-Ling, additional, Wang, Kuan-Chin, additional, Chang, Chih-Long, additional, Chen, Chih-Ping, additional, Chen, Tze-Chien, additional, Huang, Jian-Pei, additional, Huang, Ming-Chao, additional, Wang, Yeou-Lih, additional, Chang, Cheng-Chang, additional, Liu, Jah-Yao, additional, Su, Her-Young, additional, Wang, Yu-Chi, additional, Yu, Mu-Hsien, additional, Chu, Ching-Chuang, additional, Huang, Lee-Wen, additional, Seow, Kok-Min, additional, Lai, Tsung-Hsuan, additional, Lee, Fa-Kung, additional, Chen, Ching-Hui, additional, Liu, Wei-Min, additional, Chiou, Jyh-Shin, additional, Huang, Ben-Shian, additional, Lu, Yen-Feng, additional, Hsiao, Sheng-Mou, additional, Sun, Hsu-Dong, additional, Wu, Wen-Yih, additional, Teng, Sen-Wen, additional, Chen, Kuo-Hu, additional, Hung, Jeng-Hsiu, additional, Lai, Hung-Cheng, additional, Yuan, Chiou-Chung, additional, Hsieh, Ching-Hung, additional, Wang, Chin-Jung, additional, Chan, Chia-Hao, additional, Chang, Shing-Jyh, additional, Shih, Chuan-Chi, additional, Hung, Man-Jung, additional, Hsu, Shih-Tien, additional, Ke, Yu-Min, additional, Lu, Chien-Hsing, additional, Sun, Lou, additional, Chang, Wei-Chun, additional, Hung, Yao-Ching, additional, Lin, Wu-Chou, additional, Wang, Po-Hui, additional, Chen, Tze-Ho, additional, Wu, Meng-Hsing, additional, Li, Yiu-Tai, additional, Huang, Kuo-Feng, additional, Chuang, Fei-Chi, additional, Fu, Hung-Chun, additional, Kung, Fu-Tsai, additional, Huang, Kuan-Hui, additional, Chen, San-Nung, additional, Chiang, An-Jen, additional, Li, Ju-Yueh, additional, Liou, Wen-Shiung, additional, Lin, Li-Te, additional, Tsai, Hsiao-Wen, additional, and Tsui, Kuan-Hao, additional

Published
2016
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48. Uterine sarcoma Part I—Uterine leiomyosarcoma: The Topic Advisory Group systematic review

Author

Wen, Kuo-Chang, primary, Horng, Huann-Cheng, additional, Wang, Peng-Hui, additional, Chen, Yi-Jen, additional, Yen, Ming-Shyen, additional, Ng, Heung-Tat, additional, Chang, Yen-Hou, additional, Chang, Yi, additional, Chao, Hsiang-Tai, additional, Chao, Kuan-Chong, additional, Chuang, Chi-Mu, additional, Ho, Chi-Hong, additional, Huang, Chen-Yu, additional, Hung, Zhi-Chen, additional, Jiang, Ling-Yu, additional, Lau, Hei-Yu, additional, Li, Hsin-Yang, additional, Lin, Chi-Yao, additional, Liu, Chia-Hao, additional, Sun, Pi-Lin, additional, Twu, Nae-Fang, additional, Wu, Hua-Hsi, additional, Yu, Hann-Chin, additional, Ju, Fong-Yuan, additional, Tsai, Chih-Ping, additional, Chang, Wen-Hsun, additional, Hsu, Yen-Mei, additional, Lee, Na-Rong, additional, Chen, Chih-Yao, additional, Chang, Ting-Chen, additional, Chang, Wen-Chun, additional, Chen, Chii-Hou, additional, Chen, Ruey-Jian, additional, Chow, Song-Nan, additional, Lien, Yih-Ron, additional, Sheu, Bor-Ching, additional, Torng, Pao-Ling, additional, Yen, Men-Luh, additional, Lee, Wen-Ling, additional, Wang, Kuan-Chin, additional, Chang, Chih-Long, additional, Chen, Chih-Ping, additional, Chen, Jen-Ruei, additional, Chen, Tze-Chien, additional, Huang, Jian-Pei, additional, Huang, Ming-Chao, additional, Wang, Yeou-Lih, additional, Chang, Cheng-Chang, additional, Liu, Jah-Yao, additional, Su, Her-Young, additional, Wang, Yu-Chi, additional, Yu, Mu-Hsien, additional, Huang, Lee-Wen, additional, Seow, Kok-Min, additional, Lai, Tsung-Hsuan, additional, Lee, Fa-Kung, additional, Chen, Ching-Hui, additional, Huang, Ben-Shian, additional, Hsiao, Sheng-Mou, additional, Sun, Hsu-Dong, additional, Wu, Wen-Yih, additional, Teng, Sen-Wen, additional, Chen, Kuo-Hu, additional, Hung, Jeng-Hsiu, additional, Lai, Hung-Cheng, additional, Yuan, Chiou-Chung, additional, Hsieh, Ching-Hung, additional, Wang, Chin-Jung, additional, Chang, Shing-Jyh, additional, Hung, Man-Jung, additional, Hsu, Shih-Tien, additional, Ke, Yu-Min, additional, Lu, Chien-Hsing, additional, Sun, Lou, additional, Chang, Wei-Chun, additional, Hung, Yao-Ching, additional, Lin, Wu-Chou, additional, Wang, Po-Hui, additional, Chen, Tze-Ho, additional, Li, Yiu-Tai, additional, Wu, Meng-Hsing, additional, Huang, Kuo-Feng, additional, Fu, Hung-Chun, additional, Chen, San-Nung, additional, Chiang, An-Jen, additional, Li, Ju-Yueh, additional, Lin, Li-Te, additional, Tsai, Hsiao-Wen, additional, and Tsui, Kuan-Hao, additional

Published
2016
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49. Uterine sarcoma Part II—Uterine endometrial stromal sarcoma: The TAG systematic review

Author

Horng, Huann-Cheng, primary, Wen, Kuo-Chang, additional, Wang, Peng-Hui, additional, Chen, Yi-Jen, additional, Yen, Ming-Shyen, additional, Ng, Heung-Tat, additional, Chang, Yen-Hou, additional, Chang, Yi, additional, Chao, Hsiang-Tai, additional, Chao, Kuan-Chong, additional, Chuang, Chi-Mu, additional, Ho, Chi-Hong, additional, Huang, Chen-Yu, additional, Hung, Zhi-Chen, additional, Jiang, Ling-Yu, additional, Lau, Hei-Yu, additional, Li, Hsin-Yang, additional, Lin, Chi-Yao, additional, Liu, Chia-Hao, additional, Sun, Pi-Lin, additional, Twu, Nae-Fang, additional, Wu, Hua-Hsi, additional, Yu, Hann-Chin, additional, Ju, Fong-Yuan, additional, Tsai, Chih-Ping, additional, Chang, Wen-Hsun, additional, Hsu, Yen-Mei, additional, Lee, Na-Rong, additional, Chen, Chih-Yao, additional, Chang, Ting-Chen, additional, Chang, Wen-Chun, additional, Chen, Chii-Hou, additional, Chen, Ruey-Jian, additional, Chow, Song-Nan, additional, Lien, Yih-Ron, additional, Sheu, Bor-Ching, additional, Torng, Pao-Ling, additional, Yen, Men-Luh, additional, Lee, Wen-Ling, additional, Wang, Kuan-Chin, additional, Chang, Chih-Long, additional, Chen, Chih-Ping, additional, Chen, Jen-Ruei, additional, Chen, Tze-Chien, additional, Huang, Jian-Pei, additional, Huang, Ming-Chao, additional, Wang, Yeou-Lih, additional, Chang, Cheng-Chang, additional, Liu, Jah-Yao, additional, Su, Her-Young, additional, Wang, Yu-Chi, additional, Yu, Mu-Hsien, additional, Huang, Lee-Wen, additional, Seow, Kok-Min, additional, Lai, Tsung-Hsuan, additional, Lee, Fa-Kung, additional, Chen, Ching-Hui, additional, Huang, Ben-Shian, additional, Hsiao, Sheng-Mou, additional, Sun, Hsu-Dong, additional, Wu, Wen-Yih, additional, Teng, Sen-Wen, additional, Chen, Kuo-Hu, additional, Hung, Jeng-Hsiu, additional, Lai, Hung-Cheng, additional, Yuan, Chiou-Chung, additional, Hsieh, Ching-Hung, additional, Wang, Chin-Jung, additional, Chang, Shing-Jyh, additional, Hung, Man-Jung, additional, Hsu, Shih-Tien, additional, Ke, Yu-Min, additional, Lu, Chien-Hsing, additional, Sun, Lou, additional, Chang, Wei-Chun, additional, Hung, Yao-Ching, additional, Lin, Wu-Chou, additional, Wang, Po-Hui, additional, Chen, Tze-Ho, additional, Li, Yiu-Tai, additional, Wu, Meng-Hsing, additional, Huang, Kuo-Feng, additional, Fu, Hung-Chun, additional, Chen, San-Nung, additional, Chiang, An-Jen, additional, Li, Ju-Yueh, additional, Lin, Li-Te, additional, Tsai, Hsiao-Wen, additional, and Tsui, Kuan-Hao, additional

Published
2016
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50. Pluripotency maintenance of amniotic fluid-derived stem cells cultured on biomaterials

Author

Wang, Pin Yu, Lee, Henry Hsin Chung, Higuchi, Akon, Ling, Qing Dong, Lin, Hong Ren, Li, Hsin Fen, Kumar, S. Suresh, Chang, Yung, Alarfaj, Abdullah A., Munusamy, Murugan A., Chen, Da Chung, Hsu, Shih Tien, Wang, Han Chow, Hsiao, Hung Yi, Wu, Gwo Jang, Wang, Pin Yu, Lee, Henry Hsin Chung, Higuchi, Akon, Ling, Qing Dong, Lin, Hong Ren, Li, Hsin Fen, Kumar, S. Suresh, Chang, Yung, Alarfaj, Abdullah A., Munusamy, Murugan A., Chen, Da Chung, Hsu, Shih Tien, Wang, Han Chow, Hsiao, Hung Yi, and Wu, Gwo Jang

Abstract

The stem cell fates of pluripotency and differentiation are regulated by not only soluble biological cues but also insoluble biochemical cues (i.e., extracellular matrix (ECM)) and the physical cues of cell culture biomaterials (i.e., elasticity). We investigated the maintenance of pluripotency and the differentiation lineages of human amniotic fluid-derived stem cells (hAFSCs) cultured on poly(vinyl alcohol-co-itaconic acid) (PVA) hydrogels grafted with several types of ECM and corresponding oligopeptides in expansion medium. hAFSCs cultured on soft PVA hydrogels (12.2 kPa) that were grafted with oligopeptides derived from fibronectin and vitronectin showed high pluripotency, which was evaluated by Oct4, Sox2 and Nanog expression. The hAFSCs grown on soft PVA hydrogels (12.2 kPa) grafted with each oligopeptide showed higher pluripotency, as assessed by Oct4 and Nanog expression, than hAFSCs grown on stiff PVA hydrogels (25.3 kPa) grafted with the same oligopeptides and a much higher pluripotency than those grown on rigid tissue-culture polystyrene dishes. Soft biomaterials appeared to be adequate to maintain the pluripotency of hAFSCs. Surprisingly, hAFSCs that showed higher pluripotency on PVA hydrogels grafted with oligopeptides derived from fibronectin and vitronectin also expressed higher levels of early differentiation markers for three germ layers in expansion medium. This result suggests that hAFSCs are heterogeneous and that this population contains highly pluripotent stem cells and stem cells that can be easily differentiated.

Published
2015

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