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1. Serum anti-PCK1 antibody levels are a prognostic factor for patients with diabetes mellitus

Author

Namiki, Toshiki, Takemoto, Minoru, Hayashi, Aiko, Yamagata, Hiroki, Ishikawa, Takahiro, Yokote, Koutaro, Li, Shu-Yang, Kubota, Masaaki, Zhang, Bo-Shi, Yoshida, Yoichi, Matsutani, Tomoo, Mine, Seiichiro, Machida, Toshio, Kobayashi, Yoshio, Terada, Jiro, Naito, Akira, Tatsumi, Koichiro, Takizawa, Hirotaka, Nakamura, Rika, Kuroda, Hideyuki, Iwadate, Yasuo, and Hiwasa, Takaki

Published
2023
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4. Combination of high anti‐SKI and low anti‐TMED5 antibody levels is preferable prognostic factor in esophageal carcinoma.

Author

Ito, Masaaki, Yajima, Satoshi, Suzuki, Takashi, Oshima, Yoko, Nanami, Tatsuki, Sumazaki, Makoto, Shiratori, Fumiaki, Takizawa, Hirotaka, Li, Shu‐Yang, Zhang, Bo‐Shi, Yoshida, Yoichi, Matsutani, Tomoo, Hiwasa, Takaki, and Shimada, Hideaki

Abstract

Given that esophageal cancer is highly malignant, the discovery of novel prognostic markers is eagerly awaited. We performed serological identification of antigens by recombinant cDNA expression cloning (SEREX) and identified SKI proto‐oncogene protein and transmembrane p24 trafficking protein 5 (TMED5) as antigens recognized by serum IgG antibodies in patients with esophageal carcinoma. SKI and TMED5 proteins were expressed in Escherichia coli, purified by affinity chromatography, and used as antigens. The serum anti‐SKI antibody (s‐SKI‐Ab) and anti‐TMED5 antibody (s‐TMED5‐Ab) levels were significantly higher in 192 patients with esophageal carcinoma than in 96 healthy donors. The presence of s‐SKI‐Abs and s‐TMED5‐Abs in the patients' sera was confirmed by western blotting. Immunohistochemical staining showed that the TMED5 protein was highly expressed in the cytoplasm and nuclear compartments of the esophageal squamous cell carcinoma tissues, whereas the SKI protein was localized predominantly in the nuclei. Regarding the overall survival in 91 patients who underwent radical surgery, the s‐SKI‐Ab‐positive and s‐TMED5‐Ab‐negative statuses were significantly associated with a favorable prognosis. Additionally, the combination of s‐SKI‐Ab‐positive and s‐TMED5‐Ab‐negative cases showed an even clearer difference in overall survival as compared with that of s‐SKI‐Ab‐negative and s‐TMED5‐Ab‐positive cases. The s‐SKI‐Ab and s‐TMED5‐Ab biomarkers are useful for diagnosing esophageal carcinoma and distinguishing between favorable and poor prognoses. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Stage-Specific Alteration and Prognostic Relationship of Serum Fumarate Hydratase Autoantibodies in Gastric Cancer.

Author

Sasajima, Natsuko, Sumazaki, Makoto, Oshima, Yoko, Ito, Masaaki, Yajima, Satoshi, Takizawa, Hirotaka, Wang, Hao, Li, Shu-Yang, Zhang, Bo-Shi, Yoshida, Yoichi, Hiwasa, Takaki, and Shimada, Hideaki

Subjects
STOMACH cancer, KREBS cycle, AUTOANTIBODIES, RECEIVER operating characteristic curves, CANCER prognosis, OVERALL survival
Abstract

The relationship between energy production and cancer is attracting attention. This study aimed to investigate the clinicopathological significance of fumarate hydratase (FH), a tricarboxylic acid cycle enzyme, in gastric cancer using autoantibodies as biomarkers. The study analyzed 116 patients who underwent gastric cancer surgery and 96 healthy controls. Preoperative serum FH autoantibody (s-FH-Ab) titers were analyzed using an immunosorbent assay with an amplified luminescent proximity homogeneous assay. Receiver operating characteristic analysis was used to determine the cutoff s-FH-Ab titer. Clinicopathological factors and prognosis were compared between the high and low s-FH-Ab groups. The s-FH-Ab levels were significantly higher in the gastric cancer group than in the control group (p = 0.01). Levels were elevated even in patients with stage I gastric cancer compared with healthy controls (p = 0.02). A low s-FH-Ab level was significantly associated with distant metastasis (p = 0.01), peritoneal dissemination (p < 0.05), and poor overall survival (p < 0.01). Multivariate analysis revealed that low s-FH-Ab levels were an independent risk factor for poor prognosis (p < 0.01). Therefore, s-FH-Ab levels may be a useful biomarker for early diagnosis and the prediction of prognosis in patients with gastric cancer. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Elevated levels of autoantibodies against DNAJC2 in sera of patients with atherosclerotic diseases

Author

Yoshida, Yoichi, Zhang, Xiao-Meng, Wang, Hao, Machida, Toshio, Mine, Seiichiro, Kobayashi, Eiichi, Adachi, Akihiko, Matsutani, Tomoo, Kamitsukasa, Ikuo, Wada, Takeshi, Aotsuka, Akiyo, Iwase, Katsuro, Tomiyoshi, Go, Nakamura, Rika, Shinmen, Natsuko, Kuroda, Hideyuki, Takizawa, Hirotaka, Kashiwado, Koichi, Shin, Hideo, Akaogi, Yuichi, Shimada, Junichiro, Nishi, Eiichiro, Ohno, Mikiko, Takemoto, Minoru, Yokote, Koutaro, Kitamura, Kenichiro, Iwadate, Yasuo, and Hiwasa, Takaki

Published
2020
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7. Combinational antibody detection approach increases the clinical validity of colorectal cancer screening

Author

Kobayashi, Sohei, primary, Hiwasa, Takaki, additional, Kitamura, Kouichi, additional, Kano, Masayuki, additional, Hoshino, Tyuji, additional, Hirano, Sho, additional, Hashimoto, Mayuko, additional, Seimiya, Masanori, additional, Shimada, Hideaki, additional, Nomura, Fumio, additional, Matsubara, Hisahiro, additional, and Matsushita, Kazuyuki, additional

Published
2023
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8. Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

Author

Hiwasa, Takaki, Wang, Hao, Goto, Ken-ichiro, Mine, Seiichiro, Machida, Toshio, Kobayashi, Eiichi, Yoshida, Yoichi, Adachi, Akihiko, Matsutani, Tomoo, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kunimatsu, Mitoshi, Kamitsukasa, Ikuo, Mori, Masahiro, Sugimoto, Kazuo, Uzawa, Akiyuki, Muto, Mayumi, Kuwabara, Satoshi, Kobayashi, Yoshio, Ohno, Mikiko, Nishi, Eiichiro, Hattori, Akiko, Yamamoto, Masashi, Maezawa, Yoshiro, Kobayashi, Kazuki, Ishibashi, Ryoichi, Takemoto, Minoru, Yokote, Koutaro, Takizawa, Hirotaka, Kishimoto, Takashi, Matsushita, Kazuyuki, Kobayashi, Sohei, Nomura, Fumio, Arasawa, Takahiro, Kagaya, Akiko, Maruyama, Tetsuro, Matsubara, Hisahiro, Tomiita, Minako, Hamanaka, Shinsaku, Imai, Yushi, Nakagawa, Tomoo, Kato, Naoya, Terada, Jiro, Matsumura, Takuma, Katsumata, Yusuke, Naito, Akira, Tanabe, Nobuhiro, Sakao, Seiichiro, Tatsumi, Koichiro, Ito, Masaaki, Shiratori, Fumiaki, Sumazaki, Makoto, Yajima, Satoshi, Shimada, Hideaki, Shirouzu, Mikako, Yokoyama, Shigeyuki, Kudo, Takashi, Doi, Hirofumi, Iwase, Katsuro, Ashino, Hiromi, Li, Shu-Yang, Kubota, Masaaki, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, and Iwadate, Yasuo

Published
2021
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10. Association of serum levels of antibodies against ALDOA and FH4 with transient ischemic attack and cerebral infarction

Author

Wang, Hao, Lu, Hao, Zhang, Xiao-Meng, Goto, Ken-ichiro, Kobayashi, Eiichi, Yoshida, Yoichi, Adachi, Akihiko, Matsutani, Tomoo, Iwadate, Yasuo, Mine, Seiichiro, Machida, Toshio, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kamitsukasa, Ikuo, Wada, Takeshi, Aotsuka, Akiyo, Sugimoto, Kazuo, Takizawa, Hirotaka, Kashiwado, Koichi, Shin, Hideo, Tomiyoshi, Go, Nakamura, Rika, Shinmen, Natsuko, Kuroda, Hideyuki, Xu, Anding, and Hiwasa, Takaki

Published
2021
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11. Serum anti-AP3D1 antibodies are risk factors for acute ischemic stroke related with atherosclerosis

Author

Li, Shu-Yang, Yoshida, Yoichi, Kobayashi, Eiichi, Kubota, Masaaki, Matsutani, Tomoo, Mine, Seiichiro, Machida, Toshio, Maezawa, Yoshiro, Takemoto, Minoru, Yokote, Koutaro, Kobayashi, Yoshio, Takizawa, Hirotaka, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kobayashi, Sohei, Matsushita, Kazuyuki, Nomura, Fumio, Matsubara, Hisahiro, Sumazaki, Makoto, Ito, Masaaki, Yajima, Satoshi, Shimada, Hideaki, Iwase, Katsuro, Ashino, Hiromi, Wang, Hao, Goto, Kenichiro, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, Iwadate, Yasuo, and Hiwasa, Takaki

Published
2021
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13. Utility of atherosclerosis-associated serum antibodies against colony-stimulating factor 2 in predicting the onset of acute ischemic stroke and prognosis of colorectal cancer

Author

Li, Shu-Yang, primary, Yoshida, Yoichi, additional, Kubota, Masaaki, additional, Zhang, Bo-Shi, additional, Matsutani, Tomoo, additional, Ito, Masaaki, additional, Yajima, Satoshi, additional, Yoshida, Kimihiko, additional, Mine, Seiichiro, additional, Machida, Toshio, additional, Hayashi, Aiko, additional, Takemoto, Minoru, additional, Yokote, Koutaro, additional, Ohno, Mikiko, additional, Nishi, Eiichiro, additional, Kitamura, Kenichiro, additional, Kamitsukasa, Ikuo, additional, Takizawa, Hirotaka, additional, Sata, Mizuki, additional, Yamagishi, Kazumasa, additional, Iso, Hiroyasu, additional, Sawada, Norie, additional, Tsugane, Shoichiro, additional, Iwase, Katsuro, additional, Shimada, Hideaki, additional, Iwadate, Yasuo, additional, and Hiwasa, Takaki, additional

Published
2023
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15. The combination of positive anti‑WDR1 antibodies with negative anti‑CFL1 antibodies in serum is a poor prognostic factor for patients with esophageal carcinoma

Author

Ito, Masaaki, primary, Yajima, Satoshi, additional, Suzuki, Takashi, additional, Oshima, Yoko, additional, Nanami, Tatsuki, additional, Sumazaki, Makoto, additional, Shiratori, Fumiaki, additional, Wang, Hao, additional, Hu, Liubing, additional, Takizawa, Hirotaka, additional, Li, Shu-Yang, additional, Iwadate, Yasuo, additional, Hiwasa, Takaki, additional, and Shimada, Hideaki, additional

Published
2023
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16. Serum anti‑TSTD2 antibody as a biomarker for atherosclerosis‑induced ischemic stroke and chronic kidney disease

Author

Kubota, Masaaki, primary, Zhang, Bo-Shi, additional, Li, Shu-Yang, additional, Yoshida, Yoichi, additional, Wang, Hao, additional, Adachi, Akihiko, additional, Matsutani, Tomoo, additional, Mine, Seiichiro, additional, Machida, Toshio, additional, Kamitsukasa, Ikuo, additional, Wada, Takeshi, additional, Aotsuka, Akiyo, additional, Kitamura, Kenichiro, additional, Takizawa, Hirotaka, additional, Kuroda, Hideyuki, additional, Iwadate, Yasuo, additional, and Hiwasa, Takaki, additional

Published
2022
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19. Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer

Author

Ito, Masaaki, primary, Oji, Yusuke, additional, Adachi, Mayuko, additional, Imanishi, Rin, additional, Alzaaqi, Shouq, additional, Hiwasa, Takaki, additional, Oshima, Yoko, additional, Yajima, Satoshi, additional, Suzuki, Takashi, additional, Nanami, Tatsuki, additional, Sumazaki, Makoto, additional, Shiratori, Fumiaki, additional, Funahashi, Kimihiko, additional, Sugiayama, Haruo, additional, and Shimada, Hideaki, additional

Published
2022
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20. MPC-8 Serum anti-zinc finger FYVE domain-containing protein 21 (ZFYVE21) autoantibody as a novel biomarker for oligodendroglioma IDH-mutant and 1p/19q co-deletion

Author

Matsutani, Tomoo, primary, Boshi, Zhang, additional, Hirono, Seiichiro, additional, Nagane, Motoo, additional, Yoshino, Atsuo, additional, Tanaka, Shouta, additional, Ishikawa, Eiichi, additional, Fukami, Shinziro, additional, Yamaguchi, Fumio, additional, Li, Shuyang, additional, Watanabe, Yousuke, additional, Kobayashi, Masayoshi, additional, Ikegami, Shiro, additional, Hiwasa, Takaki, additional, and Iwadate, Yasuo, additional

Published
2021
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21. Identification of serum anti‑striatin 4 antibodies as a common marker for esophageal cancer and other solid cancers

Author

Ito, Masaaki, primary, Hiwasa, Takaki, additional, Oshima, Yoko, additional, Yajima, Satoshi, additional, Suzuki, Takashi, additional, Nanami, Tatsuki, additional, Sumazaki, Makoto, additional, Shiratori, Fumiaki, additional, Funahashi, Kimihiko, additional, Takizawa, Hirotaka, additional, Kashiwado, Koichi, additional, Tochigi, Naobumi, additional, and Shimada, Hideaki, additional

Published
2021
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22. Association of Serum Anti-PCSK9 Antibody Levels with Favorable Postoperative Prognosis in Esophageal Cancer

Author

Ito, Masaaki, primary, Hiwasa, Takaki, additional, Oshima, Yoko, additional, Yajima, Satoshi, additional, Suzuki, Takashi, additional, Nanami, Tatsuki, additional, Sumazaki, Makoto, additional, Shiratori, Fumiaki, additional, Funahashi, Kimihiko, additional, Li, Shu-Yang, additional, Iwadate, Yasuo, additional, Yamagata, Hiroki, additional, Jambaljav, Byambasteren, additional, Takemoto, Minoru, additional, Yokote, Koutaro, additional, Takizawa, Hirotaka, additional, and Shimada, Hideaki, additional

Published
2021
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25. Additional file 1 of Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke

Author

Hiwasa, Takaki, Wang, Hao, Goto, Ken-ichiro, Mine, Seiichiro, Machida, Toshio, Kobayashi, Eiichi, Yoshida, Yoichi, Adachi, Akihiko, Matsutani, Tomoo, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kunimatsu, Mitoshi, Kamitsukasa, Ikuo, Mori, Masahiro, Sugimoto, Kazuo, Uzawa, Akiyuki, Muto, Mayumi, Kuwabara, Satoshi, Kobayashi, Yoshio, Ohno, Mikiko, Nishi, Eiichiro, Hattori, Akiko, Yamamoto, Masashi, Maezawa, Yoshiro, Kobayashi, Kazuki, Ishibashi, Ryoichi, Takemoto, Minoru, Yokote, Koutaro, Takizawa, Hirotaka, Kishimoto, Takashi, Matsushita, Kazuyuki, Kobayashi, Sohei, Nomura, Fumio, Arasawa, Takahiro, Kagaya, Akiko, Maruyama, Tetsuro, Matsubara, Hisahiro, Tomiita, Minako, Hamanaka, Shinsaku, Imai, Yushi, Nakagawa, Tomoo, Kato, Naoya, Terada, Jiro, Matsumura, Takuma, Katsumata, Yusuke, Naito, Akira, Tanabe, Nobuhiro, Sakao, Seiichiro, Tatsumi, Koichiro, Ito, Masaaki, Shiratori, Fumiaki, Sumazaki, Makoto, Yajima, Satoshi, Shimada, Hideaki, Shirouzu, Mikako, Yokoyama, Shigeyuki, Kudo, Takashi, Doi, Hirofumi, Iwase, Katsuro, Ashino, Hiromi, Li, Shu-Yang, Kubota, Masaaki, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, and Iwadate, Yasuo

Subjects
InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, ComputingMilieux_COMPUTERSANDEDUCATION, Data_FILES, ComputerApplications_COMPUTERSINOTHERSYSTEMS
Abstract

Additional file 1. Supplementary information.

Published
2021
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28. Serum anti‐LRPAP1 is a common biomarker for digestive organ cancers and atherosclerotic diseases

Author

Sumazaki, Makoto, primary, Shimada, Hideaki, additional, Ito, Masaaki, additional, Shiratori, Fumiaki, additional, Kobayashi, Eiichi, additional, Yoshida, Yoichi, additional, Adachi, Akihiko, additional, Matsutani, Tomoo, additional, Iwadate, Yasuo, additional, Mine, Seiichiro, additional, Machida, Toshio, additional, Kamitsukasa, Ikuo, additional, Mori, Masahiro, additional, Sugimoto, Kazuo, additional, Uzawa, Akiyuki, additional, Kuwabara, Satoshi, additional, Kobayashi, Yoshio, additional, Ohno, Mikiko, additional, Nishi, Eiichiro, additional, Maezawa, Yoshiro, additional, Takemoto, Minoru, additional, Yokote, Koutaro, additional, Takizawa, Hirotaka, additional, Kashiwado, Koichi, additional, Shin, Hideo, additional, Kishimoto, Takashi, additional, Matsushita, Kazuyuki, additional, Kobayashi, Sohei, additional, Nakamura, Rika, additional, Shinmen, Natsuko, additional, Kuroda, Hideyuki, additional, Zhang, Xiao‐Meng, additional, Wang, Hao, additional, Goto, Ken‐ichiro, additional, and Hiwasa, Takaki, additional

Published
2020
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31. Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension

Author

Li, Shu‑Yang, primary, Yoshida, Yoichi, additional, Kobayashi, Eiichi, additional, Adachi, Akihiko, additional, Hirono, Seiichiro, additional, Matsutani, Tomoo, additional, Mine, Seiichiro, additional, Machida, Toshio, additional, Ohno, Mikiko, additional, Nishi, Eiichiro, additional, Maezawa, Yoshiro, additional, Takemoto, Minoru, additional, Yokote, Koutaro, additional, Kitamura, Kenichiro, additional, Sumazaki, Makoto, additional, Ito, Masaaki, additional, Shimada, Hideaki, additional, Takizawa, Hirotaka, additional, Kashiwado, Koichi, additional, Tomiyoshi, Go, additional, Shinmen, Natsuko, additional, Nakamura, Rika, additional, Kuroda, Hideyuki, additional, Zhang, Xiao‑Meng, additional, Wang, Hao, additional, Goto, Kenichiro, additional, Iwadate, Yasuo, additional, and Hiwasa, Takaki, additional

Published
2020
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32. Circulating Anti-Sorting Nexins 16 Antibodies as an Emerging Biomarker of Coronary Artery Disease in Patients with Obstructive Sleep Apnea

Author

Katsumata, Yusuke, primary, Terada, Jiro, additional, Matsumura, Takuma, additional, Koshikawa, Ken, additional, Sakao, Seiichiro, additional, Tomiyoshi, Go, additional, Shinmen, Natsuko, additional, Nakamura, Rika, additional, Kuroda, Hideyuki, additional, Nagashima, Kengo, additional, Kobayashi, Yoshio, additional, Kobayashi, Eiichi, additional, Iwadate, Yasuo, additional, Zhang, Xiao-Meng, additional, Hiwasa, Takaki, additional, and Tatsumi, Koichiro, additional

Published
2020
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34. Autologous antibody to src-homology 3-domain GRB2-like 1 specifically increases in the sera of patients with low-grade gliomas

Author

Matsutani Tomoo, Hiwasa Takaki, Takiguchi Masaki, Oide Takashi, Kunimatsu Mitoshi, Saeki Naokatsu, and Iwadate Yasuo

Subjects
Src, SH3GL1, Autoantibody, Glioma, SEREX, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Glioma is the most common primary malignant central nervous system tumor in adult, and is usually not curable in spite of various therapeutic approaches. Clarification of the oncogenic process in its early stage is important for the diagnosis and effective therapy. Methods In the present study, we used the serological identification of antigens by recombinant cDNA expression cloning (SEREX) to explore the subtle changes of the protein expression in low-grade glioma. The levels of serum autoantibodies to the SEREX-identified glioma-related antigens were analyzed by ELISA, and the epitope site was identified using deletion mutants and overlap peptide array. Changes in the serum autoantibody levels were examined in the rat glioma model using C6 and 9 L glioma cell lines. Results We identified 31 glioma-related antigens by SEREX. Among them, the serum level of autoantibody to src-homology 3-domain GRB2-like 1 (SH3GL1) was significantly higher in patients with low-grade glioma than healthy volunteers or high-grade gliomas. The 10 amino-acids at the C-terminal were identified as the epitope site by the overlap peptide array and the ELISA using deletion mutants. The tissue expression of SH3GL1 protein increased in proportion to glioma progression. The rat glioma models confirmed the increase of anti-SH3GL1 autoantibody level in the early stage and the suppression in the late stage. Conclusion SH3GL1 may be involved in the oncogenic process of gliomas and effectively elicit an autologous antibody response in low-grade gliomas. The immunological reaction to SH3GL1 would contribute to the establishment of a novel diagnostic and therapeutic target for gliomas.

Published
2012
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35. Anti‐ FIR Δexon2, a splicing variant form of PUF 60, autoantibody is detected in the sera of esophageal squamous cell carcinoma

Author

Kobayashi, Sohei, primary, Hiwasa, Takaki, additional, Ishige, Takayuki, additional, Rahmutulla, Bahityar, additional, Kano, Masayuki, additional, Hoshino, Tyuji, additional, Minamoto, Toshinari, additional, Shimada, Hideaki, additional, Nomura, Fumio, additional, Matsubara, Hisahiro, additional, and Matsushita, Kazuyuki, additional

Published
2019
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36. Elevated levels of autoantibodies against EXD2 and PHAX in the sera of patients with chronic thromboembolic pulmonary hypertension

Author

Naito, Akira, primary, Hiwasa, Takaki, additional, Tanabe, Nobuhiro, additional, Sanada, Takayuki Jujo, additional, Sugiura, Toshihiko, additional, Shigeta, Ayako, additional, Terada, Jiro, additional, Takizawa, Hirotaka, additional, Kashiwado, Koichi, additional, Sakao, Seiichiro, additional, and Tatsumi, Koichiro, additional

Published
2019
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37. Identification of a novel SEREX antigen family, ECSA, in esophageal squamous cell carcinoma

Author

Murakami Akihiro, Hachiya Takahisa, Kurei Shunsuke, Nishimori Takanori, Yasuraoka Mari, Nakashima-Fujita Kazue, Kuboshima Mari, Shiratori Tooru, Shimada Hideaki, Kagaya Akiko, Tamura Yutaka, Nomura Fumio, Ochiai Takenori, Matsubara Hisahiro, Takiguchi Masaki, and Hiwasa Takaki

Subjects
Cytology, QH573-671
Abstract

Abstract Background Diagnosis of esophageal squamous cell carcinoma (SCC) may improve with early diagnosis. Currently it is difficult to diagnose SCC in the early stage because there is a limited number of tumor markers available. Results Fifty-two esophageal SCC SEREX antigens were identified by SEREX (serological identification of antigens by recombinant cDNA expression cloning) using a cDNA phage library and sera of patients with esophageal SCC. Sequence analysis revealed that three of these antigens were similar in amino acid sequences, and they were designated as ECSA (esophageal carcinoma SEREX antigen)-1, -2 and -3. The ECSA family was also similar to an EST clone, hepatocellular carcinoma-associated antigen 25a (HCA25a). Serum antibody levels to ECSA-1, -2 and -3 were significantly higher in patients with esophageal SCC than in healthy donors. Based on the conserved amino acid sequences, three peptides were synthesized and used for enzyme-linked immunosorbent assays (ELISA). The serum antibody levels against one of these peptides were significantly higher in patients with esophageal SCC. This peptide sequence was also conserved in FAM119A, GOSR1 and BBS5, suggesting that these are also ECSA family members. Reverse transcription followed by quantitative PCR analysis showed that the mRNA expression levels of ECSA-1, -2 and -3 and FAM119A but not of HCA25a, GOSR1 and BBS5 were frequently elevated in esophageal SCC tissues. Conclusions We have identified a new gene family designated ECSA. Serum antibodies against the conserved domain of the ECSA family may be a promising tumor marker for esophageal SCC.

Published
2011
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38. Identification of Makorin 1 as a novel SEREX antigen of esophageal squamous cell carcinoma

Author

Nomura Fumio, Kuboshima Mari, Kagaya Akiko, Yasuraoka Mari, Shiratori Tooru, Shimada Hideaki, Takiguchi Masaki, Ochiai Takenori, Matsubara Hisahiro, and Hiwasa Takaki

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Esophageal squamous cell carcinoma (SCC) represents one of the most malignant tumors. To improve the poor prognosis, it is necessary to diagnose esophageal SCC at early stages using new tumor markers. SEREX (serological identification of antigens by recombinant cDNA expression cloning) is suitable for large-scale screening of tumor antigens and has been applied for various types of human tumors. Methods Tumor markers of esophageal squamous cell carcinoma (SCC) were screened by SEREX method. The presence of serum anti-makorin 1 (MKRN1) antibodies (s-MKRN1-Abs) was examined by Western blotting using bacterially expressed MKRN1 protein. The expression levels of MKRN1 mRNA in tissues were examined by RT-PCR. The biological activity of MKRN1 was examined by transfection of ras-NIH3T3 mouse fibroblasts with MKRN1 cDNA. Major ubiquitinated proteins in MKRN1-transfected cells were identified by immunoprecipitation with anti-ubiquitin antibody followed by mass spectrometry. Results MKRN1 was identified as a novel SEREX antigen of esophageal SCC. Although a total of 18 (25%) of 73 patients with esophageal SCC had s-MKRN1-Abs, none of the 43 healthy donors had a detectable level of s-MKRN1-Abs. There was no correlation between the presence of s-MKRN1-Abs and clinicopathological variables other than histological grading. Well-differentiated tumors were associated significantly with the presence of s-MKRN1-Abs in the patients. The mRNA levels of MKRN1 were frequently higher in esophageal SCC tissues than in the peripheral normal esophageal mucosa. Stable transfection of ras-NIH3T3 cells with MKRN1 cDNA induced prominent morphological changes such as enlargement of the cell body and spreading. Ubiquitination of 80- and 82-kDa proteins were clearly observed in MKRN1-transfected cells but not in the parental cells, which were identified as L-FILIP (filamin A interacting protein 1). Conclusion MKRN1 is a novel SEREX antigen of esophageal SCC, and s-NKRN1-Abs can be a candidate of diagnostic markers of esophageal SCC with high specificity. It is plausible that MKRN1 is involved in carcinogenesis of the well-differentiated type of tumors possibly via ubiquitination of L-FILIP.

Published
2009
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40. Circulating autoantibodies against neuroblastoma suppressor of tumorigenicity 1 (NBL1): A potential biomarker for coronary artery disease in patients with obstructive sleep apnea

Author

Matsumura, Takuma, primary, Terada, Jiro, additional, Kinoshita, Taku, additional, Sakurai, Yoriko, additional, Yahaba, Misuzu, additional, Tsushima, Kenji, additional, Sakao, Seiichiro, additional, Nagashima, Kengo, additional, Ozaki, Toshinori, additional, Kobayashi, Yoshio, additional, Hiwasa, Takaki, additional, and Tatsumi, Koichiro, additional

Published
2018
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41. Identification of specific and common diagnostic antibody markers for gastrointestinal cancers by SEREX screening using testis cDNA phage library

Author

Kobayashi, Sohei, primary, Hiwasa, Takaki, additional, Arasawa, Takahiro, additional, Kagaya, Akiko, additional, Ishii, Sayaka, additional, Shimada, Hideaki, additional, Ito, Masaaki, additional, Suzuki, Masae, additional, Kano, Masayuki, additional, Rahmutulla, Bahityar, additional, Kitamura, Kouichi, additional, Sawabe, Yuji, additional, Shin, Hideo, additional, Takiguchi, Masaki, additional, Nomura, Fumio, additional, Matsubara, Hisahiro, additional, and Matsushita, Kazuyuki, additional

Published
2018
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43. Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction

Author

Wang, Hao, primary, Zhang, Xiao-Meng, additional, Tomiyoshi, Go, additional, Nakamura, Rika, additional, Shinmen, Natsuko, additional, Kuroda, Hideyuki, additional, Kimura, Risa, additional, Mine, Seiichiro, additional, Kamitsukasa, Ikuo, additional, Wada, Takeshi, additional, Aotsuka, Akiyo, additional, Yoshida, Yoichi, additional, Kobayashi, Eiichi, additional, Matsutani, Tomoo, additional, Iwadate, Yasuo, additional, Sugimoto, Kazuo, additional, Mori, Masahiro, additional, Uzawa, Akiyuki, additional, Muto, Mayumi, additional, Kuwabara, Satoshi, additional, Takemoto, Minoru, additional, Kobayashi, Kazuki, additional, Kawamura, Harukiyo, additional, Ishibashi, Ryoichi, additional, Yokote, Koutaro, additional, Ohno, Mikiko, additional, Chen, Po-Min, additional, Nishi, Eiichiro, additional, Ono, Koh, additional, Kimura, Takeshi, additional, Machida, Toshio, additional, Takizawa, Hirotaka, additional, Kashiwado, Koichi, additional, Shimada, Hideaki, additional, Ito, Masaaki, additional, Goto, Ken-Ichiro, additional, Iwase, Katsuro, additional, Ashino, Hiromi, additional, Taira, Akiko, additional, Arita, Emiko, additional, Takiguchi, Masaki, additional, and Hiwasa, Takaki, additional

Published
2017
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44. Elevation of autoantibody level against PDCD11 in patients with transient ischemic attack

Author

Yoshida, Yoichi, primary, Wang, Hao, additional, Hiwasa, Takaki, additional, Machida, Toshio, additional, Kobayashi, Eiichi, additional, Mine, Seiichiro, additional, Tomiyoshi, Go, additional, Nakamura, Rika, additional, Shinmen, Natsuko, additional, Kuroda, Hideyuki, additional, Takizawa, Hirotaka, additional, Kashiwado, Koichi, additional, Kamitsukasa, Ikuo, additional, Shin, Hideo, additional, Wada, Takeshi, additional, Aotsuka, Akiyo, additional, Nishi, Eiichiro, additional, Ohno, Mikiko, additional, Takemoto, Minoru, additional, Yokote, Koutaro, additional, Takahashi, Sho, additional, Matsushima, Jun, additional, Zhang, Xiao-Meng, additional, Takiguchi, Masaki, additional, and Iwadate, Yasuo, additional

Published
2017
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45. Identification of novel serum autoantibodies against EID3 in non-functional pancreatic neuroendocrine tumors

Author

Hontani, Koji, primary, Tsuchikawa, Takahiro, additional, Hiwasa, Takaki, additional, Nakamura, Toru, additional, Ueno, Takashi, additional, Kushibiki, Toshihiro, additional, Takahashi, Mizuna, additional, Inoko, Kazuho, additional, Takano, Hironobu, additional, Takeuchi, Satoshi, additional, Dosaka-Akita, Hirotoshi, additional, Kuwatani, Masaki, additional, Sakamoto, Naoya, additional, Hatanaka, Yutaka, additional, Mitsuhashi, Tomoko, additional, Shimada, Hideaki, additional, Shichinohe, Toshiaki, additional, and Hirano, Satoshi, additional

Published
2017
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47. Identification of Novel Serum Biomarkers for Prediction of Clinical Course in Patients with Ulcerative Colitis

Author

Hamanaka, Shinsaku, primary, Nakagawa, Tomoo, additional, Hiwasa, Takaki, additional, Ohta, Yuki, additional, Kasamatsu, Shingo, additional, Ishigami, Hideaki, additional, Taida, Takashi, additional, Okimoto, Kenichiro, additional, Saito, Keiko, additional, Maruoka, Daisuke, additional, Matsumura, Tomoaki, additional, Arai, Makoto, additional, Takizawa, Hirotaka, additional, Matsushita, Kazuyuki, additional, and Katsuno, Tatsuro, additional

Published
2017
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48. Circulating Anti-Coatomer Protein Complex Subunit Epsilon (COPE) Autoantibodies as a Potential Biomarker for Cardiovascular and Cerebrovascular Events in Patients with Obstructive Sleep Apnea

Author

Matsumura, Takuma, primary, Terada, Jiro, additional, Kinoshita, Taku, additional, Sakurai, Yoriko, additional, Yahaba, Misuzu, additional, Ema, Ryogo, additional, Amata, Atsuko, additional, Sakao, Seiichiro, additional, Nagashima, Kengo, additional, Tatsumi, Koichiro, additional, and Hiwasa, Takaki, additional

Published
2017
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49. Anti-FIRs (PUF60) auto-antibodies are detected in the sera of early-stage colon cancer patients

Author

Kobayashi, Sohei, primary, Hoshino, Tyuji, additional, Hiwasa, Takaki, additional, Satoh, Mamoru, additional, Rahmutulla, Bahityar, additional, Tsuchida, Sachio, additional, Komukai, Yuji, additional, Tanaka, Tomoaki, additional, Matsubara, Hisahiro, additional, Shimada, Hideaki, additional, Nomura, Fumio, additional, and Matsushita, Kazuyuki, additional

Published
2016
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50. Anti‐FIRΔexon2, a splicing variant form of PUF60, autoantibody is detected in the sera of esophageal squamous cell carcinoma.

Author

Kobayashi, Sohei, Hiwasa, Takaki, Ishige, Takayuki, Rahmutulla, Bahityar, Kano, Masayuki, Hoshino, Tyuji, Minamoto, Toshinari, Shimada, Hideaki, Nomura, Fumio, Matsubara, Hisahiro, and Matsushita, Kazuyuki

Abstract

Anti‐PUF60 autoantibodies are reportedly detected in the sera of patients with dermatomyositis and Sjögren's syndrome; however, little is known regarding its existence in the sera of cancer patients. FIR, a splicing variant of the PUF60 gene, is a transcriptional repressor of c‐myc. In colorectal cancer, there is an overexpression of the dominant negative form of FIR, in which exon 2 is lacking (FIRΔexon2). Previously, large‐scale SEREX (serological identification of antigens by recombinant cDNA expression cloning) screenings have identified anti‐FIR autoantibodies in the sera of cancer patients. In the present study, we revealed the presence and significance of anti‐FIR (FIR/FIRΔexon2) Abs in the sera of patients with esophageal squamous cell carcinoma (ESCC). Our results were validated by an amplified luminescence proximity homogeneous assay using sera of patients with various cancer types. We revealed that anti‐FIRΔexon2 Ab had higher sensitivity than anti‐FIR Ab. Receiver operating characteristic (ROC) analysis was applied for evaluating the use of anti‐FIRΔexon2 Ab as candidate markers such as anti‐p53 Ab and carcinoembryonic antigen, and the highest area under the ROC curve was observed in the combination of anti‐FIRΔexon2 Ab and anti‐p53 Ab. In summary, our results suggest the use of anti‐FIRΔexon2 Ab in combination with the anti‐p53 Ab as a predictive marker for ESCC. The area under the ROC curve was further increased in the advanced stage of ESCC. The value of anti‐FIRΔexon2 autoantibody as novel clinical indicator against ESCC and as a companion diagnostic tool is discussed. [ABSTRACT FROM AUTHOR]

Published
2019
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