Search

Your search keyword '"Hao, Kaiqiang"' showing total 15 results
Start Over Author "Hao, Kaiqiang" Search Limiters Full Text
15 results on '"Hao, Kaiqiang"'

4. ZmmiR398b negatively regulates maize resistance to sugarcane mosaic virus infection by targeting ZmCSD2/4/9.

Author

Gao, Xinran, Du, Zhichao, Hao, Kaiqiang, Zhang, Sijia, Li, Jian, Guo, Jinxiu, Wang, Zhiping, Zhao, Shixue, Sang, Lijun, An, Mengnan, Xia, Zihao, and Wu, Yuanhua

Subjects
MOSAIC viruses, VIRUS diseases, SUGARCANE, REACTIVE oxygen species, COPPER
Abstract

MicroRNAs (miRNAs) are widely involved in various biological processes of plants and contribute to plant resistance against various pathogens. In this study, upon sugarcane mosaic virus (SCMV) infection, the accumulation of maize (Zea mays) miR398b (ZmmiR398b) was significantly reduced in resistant inbred line Chang7‐2, while it was increased in susceptible inbred line Mo17. Degradome sequencing analysis coupled with transient co‐expression assays revealed that ZmmiR398b can target Cu/Zn‐superoxidase dismutase2 (ZmCSD2), ZmCSD4, and ZmCSD9 in vivo, of which the expression levels were all upregulated by SCMV infection in Chang7‐2 and Mo17. Moreover, overexpressing ZmmiR398b (OE398b) exhibited increased susceptibility to SCMV infection, probably by increasing reactive oxygen species (ROS) accumulation, which were consistent with ZmCSD2/4/9‐silenced maize plants. By contrast, silencing ZmmiR398b (STTM398b) through short tandem target mimic (STTM) technology enhanced maize resistance to SCMV infection and decreased ROS levels. Interestingly, copper (Cu)‐gradient hydroponic experiments demonstrated that Cu deficiency promoted SCMV infection while Cu sufficiency inhibited SCMV infection by regulating accumulations of ZmmiR398b and ZmCSD2/4/9 in maize. These results revealed that manipulating the ZmmiR398b‐ZmCSD2/4/9‐ROS module provides a prospective strategy for developing SCMV‐tolerant maize varieties. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Parametric Modeling of Part and Component in Cartesian Transfer Robot based on CATIA

Author

Tian Yuan, Ping Xueliang, Hao Kaiqiang, Jiang Yi, and Chen Hong

Subjects
Mechanical engineering and machinery, TJ1-1570
Abstract

Based on four degrees of freedom cartesian transfer robot structural model,the method of parametric modeling of the main part and component of the cartesian robot are studied.Considering the actual needs of different production lines,and combined with CATIA virtual design technology and knowledge engineering techniques,the issues of cartesian robot three-dimensional model rapid generation is solved.The three-dimensional standard part and component library is built by CATIA V5R19.The selection of components and automatic generation are achieved,the designer can obtain the structural parameters by considering the user requirements and do some design calculations relevant to the selected design parameter.

Published
2015
Full Text
View/download PDF

8. Additional file 1 of Field application of nanoliposomes delivered quercetin by inhibiting specific hsp70 gene expression against plant virus disease

Author

Wang, Jie, Hao, Kaiqiang, Yu, Fangfei, Shen, Lili, Wang, Fenglong, Yang, Jinguang, and Su, Chenyu

Abstract

Additional file 1. Material in qRT-PCR, western and field application. Quantitative detection of NbHsp70 gene and TMV-CP gene. Table S1. Primer pairs used to detect gene RNA accumulation. Quantitative detection of Hsp70 protein and TMV-CP protein. Methods of disease grading. Figure S1. The standard curve of quercetin. Figure S2. The zeta potential (A), particle size (B), PDI C and quercetin concentration D of H-TQ-NP solutions with different HACC concentrations; bars with the same letters show no significant differences (LSD test, p

Published
2022
Full Text
View/download PDF

10. RNA-seq analysis of the response of plant-pathogenic oomycete Phytophthora parasitica to the fungicide dimethomorph

Author

Hao, Kaiqiang, Lin, Beisen, Nian, Fuzhao, Gao, Xi, Wei, Zhong, Luo, Gang, Lu, Yachun, Lan, Mingxian, Yang, Jinguang, and Wu, Guoxing

Subjects
Dimethomorph, Dimetomorf, Phytophthora parasitica, RNA-seq
Abstract

Phytophthora parasitica is an important oomycete that causes disease in a variety of plants, dimethomorph fungicides being specific for oomycetes. The aim of this study was to use RNA-seq to rapidly discover the mechanism by which dimethomorph acts in the treatment of P. parasitica. We found that the expression of 832 genes changed significantly after the dimethomorph treatment, including 365 up-regulated genes and 467 down-regulated genes. According to the Gene Ontology (GO) enrichment analysis, pathway enrichment and verification test results, the following conclusions are obtained: (i) the treatment of P. parasitica with dimethomorph causes changes in the expression levels of genes associated with the cell wall and cell wall synthesis; (ii) dimethomorph treatment results in reduced permeability of the cell membrane and changes in the expression of certain transport-related proteins; (iii) dimethomorph treatment increased reactive oxygen species and reduced the expression of genes related to the control of oxidative stress. Phytophthora parasitica es un importante oomiceto que origina enfermedades en una variedad de plantas; el fungicida dimetomorf es específico contra oomicetos. El objetivo de este estudio fue utilizar la tecnología de RNA-seq para descubrir rápidamente el mecanismo por el que el dimetomorf actúa en el tratamiento de P. parasitica. Descubrimos que la expresión de 832 genes se modificaba significativamente tras el tratamiento con dimetomorf, incluyendo 365 genes que son sobrerregulados y 467 genes que son subrregulados. El análisis de enriquecimiento de ontología de genes (GO), análisis de enriquecimiento de las vías y pruebas de verificación permitieron extraer las conclusiones siguientes: 1) el tratamiento de P. parasitica con dimetomorf origina cambios en los niveles de expresión de los genes relacionados con la pared celular y su síntesis; 2) el tratamiento con dimetomorf origina una reducción de la permeabilidad de la membrana celular, así como cambios en la expresión de ciertas proteínas relacionadas con el transporte, y 3) el tratamiento con dimetomorf incrementó las especies reactivas del oxígeno y redujo la expresión de los genes relacionados con el control del estrés oxidativo.

Published
2019

11. RNA-seq analysis of the response of plant-pathogenic oomycete Phytophthora parasitica to the fungicide dimethomorph

Author

Lan Mingxian, Wei Zhong, Luo Gang, Lin Beisen, Xi Gao, Hao Kaiqiang, Jinguang Yang, Nian Fuzhao, Lu Yachun, and Wu Guoxing

Subjects
Microbiology (medical), Phytophthora, Cell Membrane Permeability, beta-Glucans, Morpholines, RNA-Seq, Biology, medicine.disease_cause, Real-Time Polymerase Chain Reaction, Microbiology, Cell membrane, Cell wall, 03 medical and health sciences, Cell Wall, medicine, RNA, Messenger, Gene, Plant Diseases, Oomycete, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, 030306 microbiology, fungi, General Medicine, biology.organism_classification, Fungicides, Industrial, Fungicide, Oxidative Stress, medicine.anatomical_structure, Gene Ontology, chemistry, Gene Expression Regulation, Carrier Proteins, Reactive Oxygen Species, Sequence Alignment, Oxidative stress
Abstract

Phytophthora parasitica is an important oomycete that causes disease in a variety of plants, dimethomorph fungicides being specific for oomycetes. The aim of this study was to use RNA-seq to rapidly discover the mechanism by which dimethomorph acts in the treatment of P. parasitica. We found that the expression of 832 genes changed significantly after the dimethomorph treatment, including 365 up-regulated genes and 467 down-regulated genes. According to the Gene Ontology (GO) enrichment analysis, pathway enrichment and verification test results, the following conclusions are obtained: (i) the treatment of P. parasitica with dimethomorph causes changes in the expression levels of genes associated with the cell wall and cell wall synthesis; (ii) dimethomorph treatment results in reduced permeability of the cell membrane and changes in the expression of certain transport-related proteins; (iii) dimethomorph treatment increased reactive oxygen species and reduced the expression of genes related to the control of oxidative stress.

Published
2018

14. CLC‐Nt1 affects Potato Virus Y infection via regulation of endoplasmic reticulum luminal Ph.

Author

Sun, Hangjun, Shen, Lili, Qin, Yuanxia, Liu, Xiaowei, Hao, Kaiqiang, Li, Ying, Wang, Jie, Yang, Jinguang, and Wang, Fenglong

Subjects
CHLORIDE channels, ION channels, POTATO virus Y, GENE expression, ENDOPLASMIC reticulum, PLANT cytology, BIOLUMINESCENCE, PLANTS
Abstract

Summary: Chloride channel (CLC) proteins are important anion transporters conserved in organisms ranging from bacteria and yeast to plants and animals. According to sequence comparison, some plant CLCs are predicted to function as Cl−/H+ antiporters, but not Cl− channels. However, no direct evidence was provided to verify the role of these plant CLCs in regulating the pH of the intracellular compartment. We identified tobacco CLC‐Nt1 interacting with the Potato virus Y (PVY) 6K2 protein. To investigate its physiological function, homologous genes of CLC‐Nt1 in Nicotiana benthamiana were knocked out using the CRISPR/Cas9 system. Complementation experiments were subsequently performed by expression of wild‐type or point‐mutated CLC‐Nt1 in knockout mutants. The data presented herein demonstrate that CLC‐Nt1 is localized at endoplasmic reticulum (ER). Using a pH‐sensitive fluorescent protein (pHluorin), we found that loss of CLC‐Nt1 function resulted in a decreased ER luminal pH. Secreted GFP (secGFP) was retained mostly in ER in knockout mutants, indicating that CLC‐Nt1 is also involved in protein secretion. PVY infection induced a rise in ER luminal pH, which was dependent on functional CLC‐Nt1. By contrast, loss of CLC‐Nt1 function inhibited PVY intracellular replication and systemic infection. We propose that PVY alters ER luminal pH for infection in a CLC‐Nt1‐dependent manner. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

15. RNA-seq analysis of the response of plant-pathogenic oomycete Phytophthora parasiticato the fungicide dimethomorph

Author

Hao, Kaiqiang, Lin, Beisen, Nian, Fuzhao, Gao, Xi, Wei, Zhong, Luo, Gang, Lu, Yachun, Lan, Mingxian, Yang, Jinguang, and Wu, Guoxing

Abstract

Phytophthora parasiticais an important oomycete that causes disease in a variety of plants, dimethomorph fungicides being specific for oomycetes. The aim of this study was to use RNA-seq to rapidly discover the mechanism by which dimethomorph acts in the treatment of P. parasitica. We found that the expression of 832 genes changed significantly after the dimethomorph treatment, including 365 up-regulated genes and 467 down-regulated genes. According to the Gene Ontology (GO) enrichment analysis, pathway enrichment and verification test results, the following conclusions are obtained: (i) the treatment of P. parasiticawith dimethomorph causes changes in the expression levels of genes associated with the cell wall and cell wall synthesis; (ii) dimethomorph treatment results in reduced permeability of the cell membrane and changes in the expression of certain transport-related proteins; (iii) dimethomorph treatment increased reactive oxygen species and reduced the expression of genes related to the control of oxidative stress.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results