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17. Revealing evolving affinity between Coulombic reversibility and hysteretic Li-Si phase transformations

Author

Ogata, Ken, Jeon, Seongho, Ko, Dong-Su, Jung, Insun, Kim, Jinhae, Ito, Kimihiko, Kubo, Yoshimi, Takei, Koichi, Saito, Shunsuke, Cho, Yonghee, Park, Hosang, Jang, Jihyun, Kim, Heegoo, Kim, Jung-Hwa, Kim, Yongsu, Koh, Meiten, Uosaki, Kohei, Doo, Seok-Gwang, Hwang, Yunil, and Han, Sung-soo

Subjects
Condensed Matter - Materials Science
Abstract

Nano-structured silicon anodes are attractive alternatives to graphite in Li-ion batteries. Despite recent remarkable progresses in numerous Si-C composites, the commercialisation with significance is still limited. One of the most critical issues remained to understand is fundamentals on Li-Si Coulombic efficiency, namely, CE. Particularly, it is key to quantitatively and qualitatively resolve CE alterations and evolutions by the various Li-Si structural changes over longer cycling. However, such work is surprisingly scarce. Here, we provide new findings that iterating the hysteretic amorphous-crystalline Li-Si phase transformations accumulatively governs CE evolutions, the manner of which is numerically distinguished from incremental amorphous Li-Si volume changes. The iterations, usually featured as capacity degradation factors, can form the most efficient CE profiles over hundreds of cycles, i.e. minimising accumulative irreversible Li consumption, among the given Li-Si reaction sequences. Combined with atomistic probing methodologies, we show that the iteration drastically alters electrochemical and structural characteristics, which is synchronised with the CE behaviours., Comment: Main body (33 pages), main figures (7 figures), supplementary information (32 pages), supplementary table (5 tables), supplementary figures (26 figures)

Published
2017

21. Production of Plant-Based, Film-Type Scaffolds Using Alginate and Corn Starch for the Culture of Bovine Myoblasts.

Author

Lee, Jun-Yeong, Kamel, Jihad, Yadav, Chandra-Jit, Yadav, Usha, Afrin, Sadia, Son, Yu-Mi, Won, So-Yeon, Han, Sung-Soo, and Park, Kyung-Mee

Subjects
ALGINIC acid, ALGINATES, MYOBLASTS, POLYCAPROLACTONE, CATTLE, BOS, IN vitro meat, CORNSTARCH
Abstract

Natural scaffolds have been the cornerstone of tissue engineering for decades, providing ideal environments for cell growth within extracellular matrices. Previous studies have favored animal-derived materials, including collagen, gelatin, and laminin, owing to their superior effects in promoting cell attachment, proliferation, and differentiation compared to non-animal scaffolds, and used immortalized cell lines. However, for cultured meat production, non-animal-derived scaffolds with edible cells are preferred. Our study represents the first research to describe plant-derived, film-type scaffolds to overcome limitations associated with previously reported thick, gel-type scaffolds completely devoid of animal-derived materials. This approach has been employed to address the difficulties of fostering bovine muscle cell survival, migration, and differentiation in three-dimensional co-cultures. Primary bovine myoblasts from Bos Taurus Coreanae were harvested and seeded on alginate (Algi) or corn-derived alginate (AlgiC) scaffolds. Scaffold functionalities, including biocompatibility and the promotion of cell proliferation and differentiation, were evaluated using cell viability assays, immunofluorescence staining, and reverse transcription-quantitative polymerase chain reaction. Our results reveal a statistically significant 71.7% decrease in production time using film-type scaffolds relative to that for gel-type scaffolds, which can be maintained for up to 7 days. Film-type scaffolds enhanced initial cell attachment owing to their flatness and thinness relative to gel-type scaffolds. Algi and AlgiC film-type scaffolds both demonstrated low cytotoxicity over seven days of cell culture. Our findings indicated that PAX7 expression increased 16.5-fold in alginate scaffolds and 22.8-fold in AlgiC from day 1 to day 3. Moreover, at the differentiation stage on day 7, MHC expression was elevated 41.8-fold (Algi) and 32.7-fold (AlgiC), providing initial confirmation of the differentiation potential of bovine muscle cells. These findings suggest that both Algi and AlgiC film scaffolds are advantageous for cultured meat production. [ABSTRACT FROM AUTHOR]

Published
2024
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37. Disulfide bond-driven hyaluronic acid/sericin nanoparticles for wound-healing application.

Author

Sood, Ankur, Bhaskar, Rakesh, Won, So Yeon, Seok, Yong Joo, Kumar, Anuj, and Han, Sung Soo

Subjects
HYALURONIC acid, SERICIN, HYBRID systems, WOUND healing, URSODEOXYCHOLIC acid, TISSUE engineering, NANOPARTICLES
Abstract

There is a growing demand of bioinspired wound-healing platforms with superior tissue repairing and low immunogenicity. Hyaluronic acid (HA) and sericin (Sc) are extensively used for their competence in tissue engineering and gene/drug delivery. Herein, we demonstrate the formation of hybrid nanoparticles of HA and Sc (HS NPs) taking advantage of the dynamic disulfide bonding between their thiolated derivatives. The fabricated HS NPs were loaded with ursodeoxycholic acid (UDC), a known suppressor for NF-κB expression, and explored for the cellular response of designed hybrid UDC-HS NPs. The cytotoxicity of the synthesized hybrid system was evaluated on CCD-986Sk cells, showing negligible cytotoxicity with the cell proliferation data supporting the potential of UDC-HS NPs in increasing the cell density. Moreover, UDC-HS NPs also directed enhanced antioxidant activity. Furthermore, the wound-healing capability of UDC-HS NPs was investigated through in vitro scratch assay on CCD-986Sk cells. The results obtained could provide a useful approach in designing hybrid nanoplatform with implications in wound repair and tissue regeneration. [ABSTRACT FROM AUTHOR]

Published
2023
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44. Modulation of NLRP3 inflammasomes activation contributes to improved survival and function of mesenchymal stromal cell spheroids

Author

Pham, Duc-Vinh, primary, Shrestha, Prakash, additional, Nguyen, Thi-Kem, additional, Park, Junhyeung, additional, Pandit, Mahesh, additional, Chang, Jae-Hoon, additional, Kim, Soo Young, additional, Choi, Dong-Young, additional, Han, Sung Soo, additional, Choi, Inho, additional, Park, Gyu Hwan, additional, Jeong, Jee-Heon, additional, and Park, Pil-Hoon, additional

Published
2022
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48. IgLON4 Regulates Myogenesis via Promoting Cell Adhesion and Maintaining Myotube Orientation

Author

Lim, Jeong Ho, primary, Ahmad, Khurshid, additional, Chun, Hee Jin, additional, Hwang, Ye Chan, additional, Qadri, Afsha Fatima, additional, Ali, Shahid, additional, Ahmad, Syed Sayeed, additional, Shaikh, Sibhghatulla, additional, Choi, Jungseok, additional, Kim, Jihoe, additional, Jin, Jun-O, additional, Kim, Myunghee, additional, Han, Sung Soo, additional, Choi, Inho, additional, and Lee, Eun Ju, additional

Published
2022
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