267 results on '"Halsall, Crispin"'
Search Results
2. Occurrence and seasonal variations of antibiotic micro-pollutants in the Wei River, China
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Cao, Shengkai, Zhang, Peng, Halsall, Crispin, Hou, Zhimin, and Ge, Linke
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- 2024
- Full Text
- View/download PDF
3. Pollution in the Arctic Ocean: An overview of multiple pressures and implications for ecosystem services
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Townhill, Bryony L., Reppas-Chrysovitsinos, Efstathios, Sühring, Roxana, Halsall, Crispin J., Mengo, Elena, Sanders, Tina, Dähnke, Kirsten, Crabeck, Odile, Kaiser, Jan, and Birchenough, Silvana N. R.
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- 2022
- Full Text
- View/download PDF
4. Recommendations for the conduct of systematic reviews in toxicology and environmental health research (COSTER)
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Whaley, Paul, Aiassa, Elisa, Beausoleil, Claire, Beronius, Anna, Bilotta, Gary, Boobis, Alan, de Vries, Rob, Hanberg, Annika, Hoffmann, Sebastian, Hunt, Neil, Kwiatkowski, Carol F., Lam, Juleen, Lipworth, Steven, Martin, Olwenn, Randall, Nicola, Rhomberg, Lorenz, Rooney, Andrew A., Schünemann, Holger J., Wikoff, Daniele, Wolffe, Taylor, and Halsall, Crispin
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- 2020
- Full Text
- View/download PDF
5. The effect of partial dissolution on sea-ice chemical transport: a combined model–observational study using poly- and perfluoroalkylated substances (PFASs)
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Thomas, Max, primary, Cate, Briana, additional, Garnett, Jack, additional, Smith, Inga J., additional, Vancoppenolle, Martin, additional, and Halsall, Crispin, additional
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- 2023
- Full Text
- View/download PDF
6. Plastic Pollution in the Arctic Marine Environment
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Horton, Alice A., Ball, Hollie L., Halsall, Crispin, Horton, Alice A., Ball, Hollie L., and Halsall, Crispin
- Published
- 2023
7. The effect of partial dissolution on sea-ice chemical transport:a combined model–observational study using poly- and perfluoroalkylated substances (PFASs)
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Thomas, Max, Cate, Briana, Garnett, Jack, Smith, Inga J., Vancoppenolle, Martin, Halsall, Crispin, Thomas, Max, Cate, Briana, Garnett, Jack, Smith, Inga J., Vancoppenolle, Martin, and Halsall, Crispin
- Abstract
We investigate the effect of partial dissolution on the transport of chemicals in sea ice. Physically plausible mechanisms are added to a brine convection model that decouples chemicals from convecting brine. The model is evaluated against a recent observational dataset where a suite of qualitatively similar chemicals (poly- and perfluoroalkylated substances, PFASs) with quantitatively different physico-chemical properties were frozen into growing sea ice. With no decoupling the model performs poorly – underestimating the measured concentrations of high-chain-length PFASs. A decoupling scheme where PFASs are decoupled from salinity as a constant fraction of their brine concentration and a scheme where decoupling is proportional to the brine salinity give better performance and bring the model into reasonable agreement with observations. A scheme where the decoupling is proportional to the internal sea-ice surface area performs poorly. All decoupling schemes capture a general enrichment of longer-chained PFASs and can produce concentrations in the uppermost sea-ice layers above that of the underlying water concentration, as observed. Our results show that decoupling from convecting brine can enrich chemical concentrations in growing sea ice and can lead to bulk chemical concentrations greater than that of the liquid from which the sea ice is growing. Brine convection modelling is useful for predicting the dynamics of chemicals with more complex behaviour than sea salt, highlighting the potential of these modelling tools for a range of biogeochemical research areas.
- Published
- 2023
8. The effect of partial dissolution on sea-ice chemical transport : a combined model–observational study using poly- and perfluoroalkylated substances (PFASs)
- Author
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Thomas, Max, Cate, Briana, Garnett, Jack, Smith, Inga J., Vancoppenolle, Martin, Halsall, Crispin, Thomas, Max, Cate, Briana, Garnett, Jack, Smith, Inga J., Vancoppenolle, Martin, and Halsall, Crispin
- Abstract
We investigate the effect of partial dissolution on the transport of chemicals in sea ice. Physically plausible mechanisms are added to a brine convection model that decouples chemicals from convecting brine. The model is evaluated against a recent observational dataset where a suite of qualitatively similar chemicals (poly- and perfluoroalkylated substances, PFASs) with quantitatively different physico-chemical properties were frozen into growing sea ice. With no decoupling the model performs poorly – underestimating the measured concentrations of high-chain-length PFASs. A decoupling scheme where PFASs are decoupled from salinity as a constant fraction of their brine concentration and a scheme where decoupling is proportional to the brine salinity give better performance and bring the model into reasonable agreement with observations. A scheme where the decoupling is proportional to the internal sea-ice surface area performs poorly. All decoupling schemes capture a general enrichment of longer-chained PFASs and can produce concentrations in the uppermost sea-ice layers above that of the underlying water concentration, as observed. Our results show that decoupling from convecting brine can enrich chemical concentrations in growing sea ice and can lead to bulk chemical concentrations greater than that of the liquid from which the sea ice is growing. Brine convection modelling is useful for predicting the dynamics of chemicals with more complex behaviour than sea salt, highlighting the potential of these modelling tools for a range of biogeochemical research areas.
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- 2023
9. Polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in UK air and deposition
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Halsall, Crispin J.
- Subjects
628.53 ,Air pollution & emissions & acid rain - Abstract
Air concentrations and deposition fluxes of PCBs and PAHs are presented for 1991 and 1992 at four UK urban centres (London, Manchester, Cardiff and Stevenge). Sampling was also carried out throughout 1993 at a rural location in NW England (Hazelrigg). This monitoring programme, part of the toxic organic micropollutants survey (TOMPS), has provided the first extensive data sets for PAHs and PCBs in the UK urban and rural atmosphere. Atmospheric PCBs and PAHs were sampled every other week at each of the sites using High-Volume air samplers, equipped with glass fibre filters to collect particulate and polyurethane foam plugs to serve as vapour adsorbents. Bulk deposition was collected every month. Furthermore, atmospheric PCBs were sampled every day at the rural site, between March and June 1994, in a preliminary attempt to investigate the influence of separate meteorological episodes. At the four urban sites mean annual SigmaPAH concentrations ranged from approximately 60 - 150 ng m-3, while the mean SigmaPCB concentration ranged from 500 - 1500 pg m-3. These concentrations are the same order of magnitude as contemporary concentrations reported in other urban areas on an international basis. The lighter, predominantly vapour phase PAHs of fluorene and phenanthrene and the lower chlorinated PCB congeners, 28 and 52, dominate the atmospheric profile for both sets of compounds respectively. SigmaPAH concentrations were actually greater in the Hazelrigg atmosphere (~ factor 2) than in Manchester. This was due to significantly higher concentrations of fluorene and phenanthrene. This site and another rural site in the NW England are under the influence of local sources. Added to this, volatilisation of these lighter compounds from secondary sources such as sediments/soils/vegetation may explain the elevated concentrations during the warmer summer months. Principal components analysis highlighted the dominance of these lighter compounds in the rural atmosphere over the urban atmospheres. At Hazelrigg the SigmaPCB concentrations were lower than the urban sites by a factor of between 3-4, reflecting the lack of point sources in the rural environment. Seasonal variations were evident for the heavier PAH in both the urban and rural atmosphere. This was characterised by elevated concentrations in the winter, possibly due to increased fuel consumption for residential heating. The lighter compounds in the urban atmosphere did not show the seasonal cycling evident in the rural atmosphere, probably due to the masking effect of increased primary emissions in the winter. The SigmaPCB concentrations showed elevated concentrations in the summer at all of the sites, the vapour phase concentrations of several prominent congeners being correlated with temperature. The more chlorinated congeners showed an increased cycling amplitude from winter to summer than the less chlorinated congeners. This may be due to them being more readily exchangeable between surfaces and the atmosphere, than the lower chlorinated congeners. Partitioning between the particulate and vapour phases for the PAHs and the PCBs appears to be controlled mainly by temperature and a compound's volatility. Total suspended particulate plays a lesser role, the partitioning behaviour (calculated partition coefficients at 20°C) for six indicator congeners being similar in both the Manchester and Hazelrigg atmospheres. As surface area available for sorption is more important than just particulate concentration it is postulated here that the amount of area available at the earth's surface, particularly if covered by vegetation, will play a more important role in vapour phase sorption/desorption than atmospheric particulate. Meteorological episodes typified by high pressure anti-cyclonic systems resulted in elevated particulate phase SigmaPCB and SigmaPAH concentrations in the Cardiff and Manchester atmospheres respectively. The use of air mass back trajectories identified three separate air masses at the Hazelrigg site, when SigmaPCB concentrations varied significantly from the sampling mean. The lack of profile differences between these air masses (originating from different areas) indicate similar sources to the atmosphere, or similar processes that occur during transport that result in a uniform profile. The mean sPAH deposition flux varied from ~5 ug m-2 d-1 at the urban sites to ~2 ug m d-1 at the rural site. The mean SigmaPCB deposition flux varied from ~0. 8 ng m-2 d-1 at the urban sites to ~0. 2 ng m-2 d-1 at the rural site. Comparison with fluxes reported at rural/remote sites in north America would suggest that Hazelrigg and a site in the Lake District in NW England represent semi-urban areas; it seems that greater distances away from urban centres are required before 'true' rural fluxes are obtained.
- Published
- 1995
10. Impacts on human health in the Arctic owing to climate-induced changes in contaminant cycling – The EU ArcRisk project policy outcome
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Pacyna, Jozef M., Cousins, Ian T., Halsall, Crispin, Rautio, Arja, Pawlak, Janet, Pacyna, Elisabeth G., Sundseth, Kyrre, Wilson, Simon, and Munthe, John
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- 2015
- Full Text
- View/download PDF
11. Increasing Accumulation of Perfluorocarboxylate Contaminants Revealed in an Antarctic Firn Core (1958–2017)
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Garnett, Jack, Halsall, Crispin, Winton, Holly, Joerss, Hanna, Mulvaney, Robert, Ebinghaus, Ralf, Frey, Markus, Jones, Anna, Leeson, Amber, Wynn, Peter, Garnett, Jack, Halsall, Crispin, Winton, Holly, Joerss, Hanna, Mulvaney, Robert, Ebinghaus, Ralf, Frey, Markus, Jones, Anna, Leeson, Amber, and Wynn, Peter
- Abstract
Perfluoroalkyl acids (PFAAs) are synthetic chemicals with a variety of industrial and consumer applications that are now widely distributed in the global environment. Here, we report the measurement of six perfluorocarboxylates (PFCA, C4–C9) in a firn (granular compressed snow) core collected from a non-coastal, high-altitude site in Dronning Maud Land in Eastern Antarctica. Snow accumulation of the extracted core dated from 1958 to 2017, a period coinciding with the advent, use, and geographical shift in the global industrial production of poly/perfluoroalkylated substances, including PFAA. We observed increasing PFCA accumulation in snow over this time period, with chemical fluxes peaking in 2009–2013 for perfluorooctanoate (PFOA, C8) and nonanoate (PFNA, C9) with little evidence of a decline in these chemicals despite supposed recent global curtailments in their production. In contrast, the levels of perfluorobutanoate (PFBA, C4) increased markedly since 2000, with the highest fluxes in the uppermost snow layers. These findings are consistent with those previously made in the Arctic and can be attributed to chlorofluorocarbon replacements (e.g., hydrofluoroethers) as an inadvertent consequence of global regulation.
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- 2022
12. Climate change influence on the levels and trends of persistent organic pollutants (POPs) and chemicals of emerging Arctic concern (CEACs) in the Arctic physical environment – a review
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Hung, Hayley, Halsall, Crispin, Ball, Hollie, Bidleman, Terry, Dachs, Jordi, De Silva, Amila, Hermanson, Mark, Kallenborn, Roland, Muir, Derek, Sühring, Roxana, Wang, Xiaoping, Wilson, Simon, Hung, Hayley, Halsall, Crispin, Ball, Hollie, Bidleman, Terry, Dachs, Jordi, De Silva, Amila, Hermanson, Mark, Kallenborn, Roland, Muir, Derek, Sühring, Roxana, Wang, Xiaoping, and Wilson, Simon
- Abstract
Climate change brings about significant changes in the physical environment in the Arctic. Increasing temperatures, sea ice retreat, slumping permafrost, changing sea ice regimes, glacial loss and changes in precipitation patterns can all affect how contaminants distribute within the Arctic environment and subsequently impact the Arctic ecosystems. In this review, we summarized observed evidence of the influence of climate change on contaminant circulation and transport among various Arctic environment media, including air, ice, snow, permafrost, fresh water and the marine environment. We have also drawn on parallel examples observed in Antarctica and the Tibetan Plateau, to broaden the discussion on how climate change may influence contaminant fate in similar cold-climate ecosystems. Significant knowledge gaps on indirect effects of climate change on contaminants in the Arctic environment, including those of extreme weather events, increase in forests fires, and enhanced human activities leading to new local contaminant emissions, have been identified. Enhanced mobilization of contaminants to marine and freshwater ecosystems has been observed as a result of climate change, but better linkages need to be made between these observed effects with subsequent exposure and accumulation of contaminants in biota. Emerging issues include those of Arctic contamination by microplastics and higher molecular weight halogenated natural products (hHNPs) and the implications of such contamination in a changing Arctic environment is explored.
- Published
- 2022
13. Pollution in the Arctic Ocean:An overview of multiple pressures and implications for ecosystem services
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Townhill, Bryony L., Reppas Chrysovitsinos, Efstathios, Sühring, Roxana, Halsall, Crispin, Mengo, Elena, Sanders, Tina, Dähnke, Kirsten, Crabeck, Odile, Kaiser, Jan, Birchenough, Silvana N. R., Townhill, Bryony L., Reppas Chrysovitsinos, Efstathios, Sühring, Roxana, Halsall, Crispin, Mengo, Elena, Sanders, Tina, Dähnke, Kirsten, Crabeck, Odile, Kaiser, Jan, and Birchenough, Silvana N. R.
- Abstract
The Arctic is undergoing unprecedented change. Observations and models demonstrate significant perturbations to the physical and biological systems. Arctic species and ecosystems, particularly in the marine environment, are subject to a wide range of pressures from human activities, including exposure to a complex mixture of pollutants, climate change and fishing activity. These pressures affect the ecosystem services that the Arctic provides. Current international policies are attempting to support sustainable exploitation of Arctic resources with a view to balancing human wellbeing and environmental protection. However, assessments of the potential combined impacts of human activities are limited by data, particularly related to pollutants, a limited understanding of physical and biological processes, and single policies that are limited to ecosystem-level actions. This manuscript considers how, when combined, a suite of existing tools can be used to assess the impacts of pollutants in combination with other anthropogenic pressures on Arctic ecosystems, and on the services that these ecosystems provide. Recommendations are made for the advancement of targeted Arctic research to inform environmental practices and regulatory decisions.
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- 2022
14. Assessing pesticide use, Human exposure and environmental fate in Nigeria
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Ogwo, Ekeoma, Halsall, Crispin, Semple, Kirk, Ogwo, Ekeoma, Halsall, Crispin, and Semple, Kirk
- Abstract
Over the years, oil has been the major source of revenue in Nigeria. Agriculture has been recommended as an alternative viable option, for this to be achieved the sustainable use of pesticides has to be key. Pesticides are used by farmers to ward off pests in farms, however it has been reported that these pesticides are inappropriately used which could adversely affect the environment and could potentially endanger humans exposed to them through their consumption of fruits and vegetables. This research aimed to investigate pesticide use among farms and its impact on the environment. 486 farmers in Akwa Ibom state were interviewed and 185 farmers representing the geo political zones of the country were equally interviewed. Field observations were also done and among other things, it revealed some farmers did not receive training on pesticide application. Soil samples were analysed to ascertain their levels of concentrations. It was found that pesticides usage is high as almost every farmer made use of pesticides, the chemicals were mostly misused. Farmers are constantly exposed to chemicals and levels of concentration of the pesticides were generally within the maximum residue levels and below the WHO recommended maximum limits and impliedly did not pose any significant threats. There is also a tendency that the area considered as ‘non-farm’ is not the case as some sections of those areas used to have farms situated in them and may not reflect the current practice, hence reasons for of the concentration levels found therein. The reported concentrations of the metabolites of DDT suggest that it is from either historical usage or the illegal usage of these pesticides or even a case of both factors. The research showed that most farmers did not receive training on pesticide application and the authorities mandated with carrying out enforcement in this regard by regulating the way it is used, need to do more.
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- 2022
15. Climate change influence on the levels and trends of persistent organic pollutants (POPs) and chemicals of emerging Arctic concern (CEACs) in the Arctic physical environment - a review
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0000-0003-0719-8948, 0000-0001-7469-0532, 0000-0002-3557-523X, 0000-0003-1703-2538, 0000-0001-6631-9776, 0000-0002-7285-8044, 0000-0001-7524-7540, Hung, Hayley, Halsall, Crispin, Ball, Hollie, Bidleman, Terry, Dachs, Jordi, De Silva, Amila, Hermanson, Mark, Kallenborn, Roland, Muir, Derek, Sühring, Roxana, Wang, Xiaoping, Wilson, Simon, 0000-0003-0719-8948, 0000-0001-7469-0532, 0000-0002-3557-523X, 0000-0003-1703-2538, 0000-0001-6631-9776, 0000-0002-7285-8044, 0000-0001-7524-7540, Hung, Hayley, Halsall, Crispin, Ball, Hollie, Bidleman, Terry, Dachs, Jordi, De Silva, Amila, Hermanson, Mark, Kallenborn, Roland, Muir, Derek, Sühring, Roxana, Wang, Xiaoping, and Wilson, Simon
- Abstract
Climate change brings about significant changes in the physical environment in the Arctic. Increasing temperatures, sea ice retreat, slumping permafrost, changing sea ice regimes, glacial loss and changes in precipitation patterns can all affect how contaminants distribute within the Arctic environment and subsequently impact the Arctic ecosystems. In this review, we summarized observed evidence of the influence of climate change on contaminant circulation and transport among various Arctic environment media, including air, ice, snow, permafrost, fresh water and the marine environment. We have also drawn on parallel examples observed in Antarctica and the Tibetan Plateau, to broaden the discussion on how climate change may influence contaminant fate in similar cold-climate ecosystems. Significant knowledge gaps on indirect effects of climate change on contaminants in the Arctic environment, including those of extreme weather events, increase in forests fires, and enhanced human activities leading to new local contaminant emissions, have been identified. Enhanced mobilization of contaminants to marine and freshwater ecosystems has been observed as a result of climate change, but better linkages need to be made between these observed effects with subsequent exposure and accumulation of contaminants in biota. Emerging issues include those of Arctic contamination by microplastics and higher molecular weight halogenated natural products (hHNPs) and the implications of such contamination in a changing Arctic environment is explored.
- Published
- 2022
16. Pollution in the Arctic Ocean: An overview of multiple pressures and implications for ecosystem services
- Author
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Townhill, Bryony L., primary, Reppas-Chrysovitsinos, Efstathios, additional, Sühring, Roxana, additional, Halsall, Crispin J., additional, Mengo, Elena, additional, Sanders, Tina, additional, Dähnke, Kirsten, additional, Crabeck, Odile, additional, Kaiser, Jan, additional, and Birchenough, Silvana N. R., additional
- Published
- 2021
- Full Text
- View/download PDF
17. Investigating the uptake and fate of poly- and perfluoroalkylated substances (PFAS) in sea ice using an experimental sea ice chamber
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Garnett, Jack, Halsall, Crispin J., Thomas, Max, Crabeck, Odile, France, James, Joerss, Hanna, Ebinghaus, Ralf, Kaiser, Jan, Leeson, Amber, Wynn, Peter, Garnett, Jack, Halsall, Crispin J., Thomas, Max, Crabeck, Odile, France, James, Joerss, Hanna, Ebinghaus, Ralf, Kaiser, Jan, Leeson, Amber, and Wynn, Peter
- Abstract
Poly- and perfluoroalkyl substances (PFAS) are contaminants of emerging Arctic concern and are present in the marine environments of the polar regions. Their input to and fate within the marine cryosphere are poorly understood. We conducted a series of laboratory experiments to investigate the uptake, distribution, and release of 10 PFAS of varying carbon chain length (C4–C12) in young sea ice grown from artificial seawater (NaClsolution). We show that PFAS are incorporated into bulk sea ice during ice formation and regression analyses for individual PFAS concentrations in bulk sea ice were linearly related to salinity (r2 = 0.30 to 0.88, n = 18, p < 0.05). This shows that their distribution is strongly governed by the presence and dynamics of brine (high salinity water) within the sea ice. Furthermore, long-chain PFAS (C8–C12), were enriched in bulk ice up to 3-fold more than short-chain PFAS (C4–C7) and NaCl. This suggests that chemical partitioning of PFAS between the different phases of sea ice also plays a role in their uptake during its formation. During sea ice melt, initial meltwater fractions were highly saline and predominantly contained short-chain PFAS, whereas the later, fresher meltwater fractions predominantly contained long-chain PFAS. Our results demonstrate that in highly saline parts of sea ice (near the upper and lower interfaces and in brine channels) significant chemical enrichment (ε) of PFAS can occur with concentrations in brine channels greatly exceeding those in seawater from which it forms (e.g., for PFOA, εrinebrine = 10 ± 4). This observation has implications for biological exposure to PFAS present in brine channels, a common feature of first-year sea ice which is the dominant ice type in a warming Arctic.
- Published
- 2021
18. Investigating the occurrence and accumulation of perfluoroalkylated substances and other persistent organic pollutants in snow and ice of the Earth’s Polar regions
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Garnett, Jack, Halsall, Crispin, Wynn, Peter, Leeson, Amber, Garnett, Jack, Halsall, Crispin, Wynn, Peter, and Leeson, Amber
- Abstract
Persistent organic pollutants (POPs) are a highly diverse group of synthetic chemicals that are released into the environment from human activities and display a host of adverse effects in wildlife and humans. Perfluoroalkylated substances (PFASs) are one major group of industrial chemicals that are globally produced in vast quantities and are subject to various global regulations. However, PFASs are present in the Earth’s Polar Regions and yet little is known about their environmental fate and behaviour, particularly their accumulation and fate in snow and ice and the wider cryosphere. Moreover, climate change is altering the cryosphere, affecting sea ice and its properties for example, which in turn may affect the biogeochemical cycling of these pollutants and could lead to altered or enhanced biological exposure and uptake. This thesis examines the accumulation of PFASs in snow and firn as a useful recorder of environmental contamination in Antarctica, a region which lacks a multi-decadal time series of air monitoring data. The thesis also investigates in detail the processes that result in the uptake, distribution and accumulation of persistent organic pollutants in sea ice, particularly in the context of a warmer Arctic, where sea ice is now dominated by brine-rich single season ice. There is a lack of monitoring data on the levels of PFASs in Antarctica, and so a snow core was taken from Kohnen Station (East Antarctica plateau) to determine the historical deposition rates in the region. Results revealed low or non-detectable levels of perfluorosulfonic acids (PFSAs) but showed increasing trends of perfluorocarboxylates (PFCAs) between 1958 – 2017. Deposition rates for PFCAs in snow varied depending on chain length, with PFBA (C4) showing up to 2-orders of magnitude higher (1.3 and 280 ng m-2 yr-1) than PFOA (C8) (1.6 and 12.6 ng m-2 yr-1). Furthermore, correlations between PFCAs of varying chain length were strong (r2 > 0.7, p < 0.01). This information suggest
- Published
- 2021
19. Applying systematic review methods in chemical risk assessment
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Whaley, Paul, Halsall, Crispin, Whaley, Paul, and Halsall, Crispin
- Abstract
Context : Chemical risk assessment has traditionally been dependent on “narrative” approaches for synthesising evidence about potential health harms from exposure to chemical substances. However, narrative reviews are recognised as being vulnerable to a range of methodological shortcomings which introduce bias and inconsistency into the summarisation of scientific evidence. This is likely to be a contributing factor in a number of controversies about the safety of chemical substances. The potential value of systematic review methods for improving the transparency and validity of chemical risk assessments was arguably first articulated in the mid-2000s. By 2015, the first major frameworks for conducting systematic reviews of environmental health evidence had been published. What was not well understood at the time was how systematic review, as a technically exacting methodology originally developed for evaluating the effectiveness of interventions in healthcare, might be adapted to the specific workflows and evidence streams of chemical risk assessment. Objectives : The aim of this Thesis is to investigate how systematic review methods can be applied to the conduct of chemical risk assessment. This overall aim is broken down into four specific objectives: to identify practical challenges and knowledge gaps which impede the implementation of systematic review methods in chemical risk assessment; to define a consensus view on key recommended practices for the planning and conduct of systematic reviews in the environmental health sciences; to examine how “biological plausibility” as a concept fundamental to risk assessment is accommodated in systematic review methodologies; and to describe the role of ontologies in making evidence accessible for use in systematic chemical assessments. Discussion : The use of systematic review methods should improve the validity, utility and transparency of chemical risk assessments. However, the successful implementation of systematic r
- Published
- 2021
20. Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)
- Author
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Strong, Rebecca J., Halsall, Crispin J., Ferenčík, Martin, Jones, Kevin C., Shore, Richard F., Martin, Francis L., Strong, Rebecca J., Halsall, Crispin J., Ferenčík, Martin, Jones, Kevin C., Shore, Richard F., and Martin, Francis L.
- Abstract
Amphibians are undergoing large population declines in many regions around the world. As environmental pollution from both agricultural and urban sources has been implicated in such declines, there is a need for a biomonitoring approach to study potential impacts on this vulnerable class of organism. This study assessed the use of infrared (IR) spectroscopy as a tool to detect changes in several tissues (liver, muscle, kidney, heart and skin) of late-stage common frog (Rana temporaria) tadpoles collected from ponds with differing water quality. Small differences in spectral signatures were revealed between a rural agricultural pond and an urban pond receiving wastewater and landfill run-off; these were limited to the liver and heart, although large differences in body size were apparent, surprisingly with tadpoles from the urban site larger than those from the rural site. Large differences in liver spectra were found between tadpoles from the pesticide and nutrient impacted pond compared to the rural agricultural pond, particularly in regions associated with lipids. Liver mass and hepatosomatic indices were found to be significantly increased in tadpoles from the site impacted by pesticides and trace organic chemicals, suggestive of exposure to environmental contamination. Significant alterations were also found in muscle tissue between tadpoles from these two ponds in regions associated with glycogen, potentially indicative of a stress response. This study highlights the use of IR spectroscopy, a low-cost, rapid and reagent-free technique in the biomonitoring of a class of organisms susceptible to environmental degradation., V mnoha oblastech světa dochází k velkému poklesu populací obojživelníků. Jelikož se za příčinu tohoto poklesu považuje znečištění životního prostředí jak ze zemědělských, tak z městských zdrojů, existuje potřeba biomonitoringu sloužícího studiu potenciálních dopadů na tuto zranitelnou skupinu organismů. Tato práce posuzuje užití IČ spektroskopie jako nástroje detekce změn ve tkáních (játra, svaly, ledviny, srdce a kůže) pulců skokana hnědého (Rana temporaria) v pozdních fázích vývoje, kteří byly odebráni z vodních nádrží s různou kvalitou vody. Malé rozdíly byly shledány mezi nádržemi venkovského zemědělství a městskými nádržemi, do kterých přitékají odpadní vody a odtoky ze skládek; tyto rozdíly byly omezeny na játra a srdce, ačkoliv byly zjevné velké rozdíly ve velikosti pulců, překvapivě pulci z městských nádrží byli větší než pulci z venkovských nádrží. Větší rozdíly ve spektrech jater byly nalezeny mezi pulci z nádrží zatížených pesticidy a živinami ve srovnání s venkovskými nádržemi. Hmotnost jater a hepatosomatické indikátory byly významně zvýšené u pulců z nádrží znečištěných pesticidy a stopovými organickými chemikáliemi, což naznačuje vystavení kontaminaci životního prostředí.Významné změny byly též nalezeny ve svalových tkáních u pulců z těchto dvou nádrží, v částech spojených s glykogenem, což potenciálně indikuje reakci na stress. Tato práce vyzdvihuje využití IČ spektroskopie, levné a rychlé analytické techniky biomonitoringu třídy organismů ovlivněných degradací životního prostředí.
- Published
- 2021
21. A survey of systematic evidence mapping practice and the case for knowledge graphs in environmental health & toxicology
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Wolffe, Taylor, Vidler, John, Halsall, Crispin, Hunt, Neil, Whaley, Paul, Wolffe, Taylor, Vidler, John, Halsall, Crispin, Hunt, Neil, and Whaley, Paul
- Abstract
Systematic evidence mapping offers a robust and transparent methodology for facilitating evidence-based approaches to decision-making in chemicals policy and wider environmental health. Interest in the methodology is growing; however, its application in environmental health is still novel. To facilitate the production of effective systematic evidence maps for environmental health use cases, we survey the successful application of evidence mapping in other fields where the methodology is more established. Focusing on issues of “data storage technology”, “data integrity”, “data accessibility”, and “transparency”, we characterise current evidence-mapping practice and critically review its potential value for environmental health contexts. We note that rigid, flat data tables and schema-first approaches dominate current mapping methods and highlight how this practice is ill-suited to the highly connected, heterogeneous and complex nature of environmental health data. We propose this challenge is overcome by storing and structuring data as “knowledge graphs”. Knowledge graphs offer a flexible, schemaless and scalable model for systematically mapping the environmental health literature. Associated technologies such as ontologies are well-suited to the long-term goals of systematic mapping methodology in promoting resource-efficient access to the wider environmental health evidence base. Several graph storage implementations are readily available, with a variety of proven use cases in other fields. Thus, developing and adapting systematic evidence mapping for environmental health should utilise these graph-based resources to ensure the production of scalable, interoperable and robust maps to aid decision-making processes in chemicals policy and wider environmental health.
- Published
- 2020
22. Evidence-based approaches to chemical risk assessment and risk management decision-making
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Wolffe, Taylor, Halsall, Crispin, Wolffe, Taylor, and Halsall, Crispin
- Abstract
Chemicals policy is designed to protect human and ecological health from the adverse effects that can result from exposure to manufactured chemical substances. It entails a complex process of regulatory chemical risk assessment and risk-management decision-making, drawing expertise from a diverse range of fields including toxicology and environmental health. However, these decision-making processes have come under increased scrutiny in recent years – criticized for bias, lack of transparency, rigor and a failure to identify unacceptable risks before widespread exposure occurs. This has resulted in calls for a more “evidence-based” approach, in which all relevant, available evidence is analyzed in a robust, transparent and reproducible manner. There is thus a growing need to incorporate methodological frameworks capable of facilitating evidence-based approaches to chemical risk assessment and regulatory decision-making. Such frameworks have been successfully developed in the field of medicine, which underwent a similar paradigm shift to that currently shaping chemical risk assessment, in the early 1990s. The gold-standard for evidence-based decision-making championed by the evidence-based medicine movement takes the form of systematic review. Systematic review describes a prescriptive and transparent method for collating, appraising and analyzing all available, relevant evidence in answer to a specific research question. By pooling the results of individual (independent) studies, systematic reviews synthesize conclusions which are not only more precise but are representative of an entire evidence-base. Now well established within clinical decision-making, the application of systematic review to chemical risk assessment is beginning to gain prominence. However, several challenges and barriers threaten to slow the uptake and quality of systematic review for chemical risk assessment. These include the prohibitively narrow focus of systematic reviews, which are at odds w
- Published
- 2020
23. A code of practice for the conduct of systematic reviews in toxicology and environmental health research (COSTER)
- Author
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Whaley, Paul, Aiassa, Elisa, Beausoleil, Claire, Beronius, Anna, Bilotta, Gary, Boobis, Alan, de Vries, Rob, Hanberg, Annika, Hoffmann, Sebastian, Hunt, Neil, Kwiatkowski, Carol, Lam, Juleen, Lipworth, Steven, Martin, Olwenn, Randall, Nicola, Rhomberg, Lorenz, Rooney, Andrew A., Schünemann, Holger J., Wikoff, Daniele, Wolffe, Taylor, and Halsall, Crispin
- Subjects
research methods ,systematic review ,chemical risk assessment ,research synthesis ,environmental health ,epidemiology ,research standards ,toxicology - Abstract
Background: There are several standards which make explicit a consensus view on sound practice in systematic reviews (SRs) for the medical sciences. Until now, no equivalent standard has been published for SRs which focus on human health risks posed by exposure to environmental challenges, chemical or otherwise. Objectives: To develop an expert, cross-sector consensus on a core set of requirements for sound practice in planning and conducting a SR in the environmental health sciences. Methods: A draft set of requirements was derived from two existing standards for SRs in biomedicine and discussed at an international workshop of 33 participants from government, industry, non-government organisations, and academia. The guidance was revised over six follow-up webinars and several rounds of email feedback, until there was group consensus that a comprehensive framework for the planning and conduct of high-quality environmental health SRs had been articulated. Results: The Conduct of Systematic Reviews in Toxicology and Environmental Health Research (COSTER) standard is a code of practice consisting of 70 requirements across eight performance domains, representing the consensus view of a diverse group of experts as to what constitutes “sound and good” practice in the conduct of environmental health SRs. Discussion: COSTER provides a set of sound-practice requirements which, if followed, should facilitate the production of credible, high-value SRs of environmental health evidence. COSTER clarifies sound and good practice in a number of controversial aspects of SR conduct, providing requirements relating to management of conflicts of interest, inclusion of grey literature, and protocol registration and publication. Not all of the practices are yet commonplace, but environmental health SRs would benefit from their introduction. Some aspects of SR, such as assessment of external validity at the level of individual study, are not yet sufficiently developed for consensus on sound practice to be achieved.
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- 2019
- Full Text
- View/download PDF
24. “Good Epidemiology Practice” Guidelines for Pesticide Exposure Assessment
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Goodman, Julie E., primary, Prueitt, Robyn L., additional, Boffetta, Paolo, additional, Halsall, Crispin, additional, and Sweetman, Andrew, additional
- Published
- 2020
- Full Text
- View/download PDF
25. Mechanistic insight into the uptake and fate of persistent organic pollutants in sea ice
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Garnett, Jack, Halsall, Crispin J., Thomas, Max, France, James, Kaiser, Jan, Graf, Carola, Leeson, Amber, Wynn, Peter, Garnett, Jack, Halsall, Crispin J., Thomas, Max, France, James, Kaiser, Jan, Graf, Carola, Leeson, Amber, and Wynn, Peter
- Abstract
The fate of persistent organic pollutants in sea ice is a poorly researched area and yet ice serves as an important habitat for organisms at the base of the marine foodweb. This study presents laboratory-controlled experiments to investigate the mechanisms governing the fate of organic contaminants in sea ice grown from artificial seawater. Sea ice formation was shown to result in the entrainment of chemicals from seawater, and concentration profiles in bulk ice generally showed the highest levels in both the upper (ice-atmosphere interface) and lower (ice-ocean interface) ice layers, suggesting their incorporation and distribution is influenced by brine advection. Results from a 1-D sea ice brine dynamics model supported this, but also indicated that other processes may be needed to accurately model low-polarity compounds in sea ice. This was reinforced by results from a melt experiment, which not only showed chemicals were more enriched in saltier brine, but also revealed that chemicals are released from sea ice at variable rates. We use our results to demonstrate the importance of processes related to the occurrence and movement of brine for controlling chemical fate in sea ice which provides a pathway for exposure to ice-associated biota at the base of the pelagic food web.
- Published
- 2019
26. Systematic evidence maps as a novel tool to support evidence-based decision-making in chemicals policy and risk management
- Author
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Wolffe, Taylor, Whaley, Paul, Halsall, Crispin, Rooney, Andrew, Walker, Vickie, Wolffe, Taylor, Whaley, Paul, Halsall, Crispin, Rooney, Andrew, and Walker, Vickie
- Abstract
Background While systematic review (SR) methods are gaining traction as a method for providing a reliable summary of existing evidence for health risks posed by exposure to chemical substances, it is becoming clear that their value is restricted to a specific range of risk management scenarios - in particular, those which can be addressed with tightly focused questions and can accommodate the time and resource requirements of a systematic evidence synthesis. Methods The concept of a systematic evidence map (SEM) is defined and contrasted to the function and limitations of systematic review (SR) in the context of risk management decision-making. The potential for SEMs to facilitate evidence-based decision-making are explored using a hypothetical example in risk management priority-setting. The potential role of SEMs in reference to broader risk management workflows is characterised. Results SEMs are databases of systematically gathered research which characterise broad features of the evidence base. Although not intended to substitute for the evidence synthesis element of systematic reviews, SEMs provide a comprehensive, queryable summary of a large body of policy relevant research. They provide an evidence-based approach to characterising the extent of available evidence and support forward looking predictions or trendspotting in the chemical risk sciences. In particular, SEMs facilitate the identification of related bodies of decision critical chemical risk information which could be further analysed using SR methods, and highlight gaps in the evidence which could be addressed with additional primary studies to reduce uncertainties in decision-making. Conclusions SEMs have strong and growing potential as a high value tool in resource efficient use of existing research in chemical risk management. They can be used as a critical precursor to efficient deployment of high quality SR methods for characterising chemical health risks. Furthermore, SEMs have potential, at
- Published
- 2019
27. Towards a non-animal risk assessment for anti-androgenic effects in humans
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Dent, Matthew, Martin, Frank, Halsall, Crispin, Carmichael, Paul, Dent, Matthew, Martin, Frank, Halsall, Crispin, and Carmichael, Paul
- Abstract
In recent years, concern has been raised over the possibility that substances in foods, consumer products and the environment are causing health effects in humans and the environment due to their ability to perturb endocrine signalling. Toxicological risk assessments therefore need to be protective for these modes of action. The accepted approach for assessing potential ‘endocrine disrupting chemicals’ (EDCs) involves the generation of laboratory animal data. The growing dissatisfaction of many scientists with the relevance of animal studies to the assessment of human risk and increasing societal demand for an end to animal testing presents challenges and opportunities in the safety evaluation of these substances. This thesis examines the opportunities to apply non-animal approaches to the risk assessment of anti-androgenic substances in consumer products. Gaps were identified that are currently preventing the adoption of such an approach, which broadly cover three areas. Firstly, under the current paradigm, in vitro alerts for anti-androgenicity invariably trigger animal testing, so an approach to allow risk-based decision making using only in vitro and exposure data is needed. Secondly, there is a lack of in vitro tools able to characterise the effects of substances affecting the hypothalamic and pituitary control of gonadotropin secretion; a search was conducted for cells that could address this. Thirdly, there are currently no in vitro models capable of distinguishing between exposures that cause adaptive changes to endocrine signalling and those that cause adverse health effects. The use of 3D prostate microtissues was therefore investigated to assess whether these would provide biomarkers to identify tipping points between adaptive and adverse responses. A tiered, exposure-led, and human-relevant risk assessment approach was developed that can be applied to safety decision making and prevent unnecessary animal use. Further developments in some of the higher-ti
- Published
- 2019
28. Systematic evidence maps as a novel tool to support evidence-based decision-making in chemicals policy and risk management
- Author
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Wolffe, Taylor A.M., primary, Whaley, Paul, additional, Halsall, Crispin, additional, Rooney, Andrew A., additional, and Walker, Vickie R., additional
- Published
- 2019
- Full Text
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29. Levels and trends of poly- and perfluoroalkyl substances in the Arctic environment – An update
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Muir, Derek, primary, Bossi, Rossana, additional, Carlsson, Pernilla, additional, Evans, Marlene, additional, De Silva, Amila, additional, Halsall, Crispin, additional, Rauert, Cassandra, additional, Herzke, Dorte, additional, Hung, Hayley, additional, Letcher, Robert, additional, Rigét, Frank, additional, and Roos, Anna, additional
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- 2019
- Full Text
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30. Assessing residual status and spatial variation of current-use pesticides under the influence of environmental factors in major cash crop growing areas of Pakistan
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Ansari, Sundas, primary, Waheed, Sidra, additional, Ali, Usman, additional, Jones, Kevin C., additional, Sweetman, Andrew James, additional, Halsall, Crispin, additional, and Malik, Riffat Naseem, additional
- Published
- 2018
- Full Text
- View/download PDF
31. Aqueous multivariate phototransformation kinetics of dissociated tetracycline : implications for the photochemical fate in surface waters
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Ge, Linke, Dong, Qianqian, Halsall, Crispin, Chen, Chang-Er L., Li, Jun, Wang, Degao, Zhang, Peng, Yao, Ziwei, Ge, Linke, Dong, Qianqian, Halsall, Crispin, Chen, Chang-Er L., Li, Jun, Wang, Degao, Zhang, Peng, and Yao, Ziwei
- Abstract
Antibiotics are ubiquitous pollutants in aquatic systems and can exist as different dissociated species depending on the water pH. New knowledge of their multivariate photochemical behavior (i.e., the photobehavior of different ionized forms) is needed to improve our understanding on the fate and possible remediation of these pharmaceuticals in surface and waste waters. In this study, the photochemical degradation of aqueous tetracycline (TC) and its dissociated forms (TCH20, TCH-, and TC2-) was investigated. Simulated sunlight experiments and matrix calculations indicated that the three dissociated species had dissimilar photolytic kinetics and photo-oxidation reactivities. TC2- photo-degraded the fastest due to apparent photolysis with a kinetic constant of 0.938 +/- 0.021 min(-1), followed by TCH- (0.020 +/- 0.005 min(-1)) and TCH20 (0.012 +/- 0.001 min(-1)), whereas Tar was found to be the most highly reactive toward center dot OH (105.78 +/- 3.40 M(-1 )s(-1)), and TC2- reacted the fastest with O-1(2) (344.96 +/- 45.07 M-1 s(-1)). Water with relatively high pH (e.g., similar to 8-9) favors the dissociated forms of TCH- and TC2- which are most susceptible to photochemical loss processes compared to neutral TC. The calculated corresponding environmental half-lives (t(1/2,E) ) in sunlit surface waters ranged from 0.05 h for pH = 9 in midsummer to 3.68 h for pH = 6 in midwinter at 45 degrees N latitude. The moms was dominated by apparent photolysis (especially in summer, 62-91%), followed by O-1(2) and center dot OH oxidation. Adjusting the pH to slightly alkaline conditions prior to UV or solar UV light treatment may be an effective way of enhancing the photochemical removal of TC from contaminated water.
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- 2018
- Full Text
- View/download PDF
32. Polychlorinated biphenyls (PCBs) as sentinels for the elucidation of Arctic environmental change processes : a comprehensive review combined with ArcRisk project results
- Author
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Carlsson, Pernilla, Breivik, Knut, Brorström-Lundén, Eva, Cousins, Ian, Christensen, Jesper, Grimalt, Joan O., Halsall, Crispin, Kallenborn, Roland, Abass, Khaled, Lammel, Gerhard, Munthe, John, MacLeod, Matthew, Øyvind Odland, Jon, Pawlak, Janet, Rautio, Arja, Reiersen, Lars-Otto, Schlabach, Martin, Stemmler, Irene, Wilson, Simon, Wöhrnschimmel, Henry, Carlsson, Pernilla, Breivik, Knut, Brorström-Lundén, Eva, Cousins, Ian, Christensen, Jesper, Grimalt, Joan O., Halsall, Crispin, Kallenborn, Roland, Abass, Khaled, Lammel, Gerhard, Munthe, John, MacLeod, Matthew, Øyvind Odland, Jon, Pawlak, Janet, Rautio, Arja, Reiersen, Lars-Otto, Schlabach, Martin, Stemmler, Irene, Wilson, Simon, and Wöhrnschimmel, Henry
- Abstract
Polychlorinated biphenyls (PCBs) can be used as chemical sentinels for the assessment of anthropogenic influences on Arctic environmental change. We present an overview of studies on PCBs in the Arctic and combine these with the findings from ArcRisk-a major European Union-funded project aimed at examining the effects of climate change on the transport of contaminants to and their behaviour of in the Arctic-to provide a case study on the behaviour and impact of PCBs over time in the Arctic. PCBs in the Arctic have shown declining trends in the environment over the last few decades. Atmospheric long-range transport from secondary and primary sources is the major input of PCBs to the Arctic region. Modelling of the atmospheric PCB composition and behaviour showed some increases in environmental concentrations in a warmer Arctic, but the general decline in PCB levels is still the most prominent feature. 'Within-Arctic' processing of PCBs will be affected by climate change-related processes such as changing wet deposition. These in turn will influence biological exposure and uptake of PCBs. The pan-Arctic rivers draining large Arctic/sub-Arctic catchments provide a significant source of PCBs to the Arctic Ocean, although changes in hydrology/sediment transport combined with a changing marine environment remain areas of uncertainty with regard to PCB fate. Indirect effects of climate change on human exposure, such as a changing diet will influence and possibly reduce PCB exposure for indigenous peoples. Body burdens of PCBs have declined since the 1980s and are predicted to decline further.
- Published
- 2018
- Full Text
- View/download PDF
33. Exploring the aquatic photodegradation of two ionisable fluoroquinolone antibiotics – Gatifloxacin and balofloxacin:Degradation kinetics, photobyproducts and risk to the aquatic environment
- Author
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Ge, Linke, Halsall, Crispin, Chen, Chang-Er, Zhang, Peng, Dong, Qianqian, Yao, Ziwei, Ge, Linke, Halsall, Crispin, Chen, Chang-Er, Zhang, Peng, Dong, Qianqian, and Yao, Ziwei
- Abstract
Fluoroquinolone antibiotics (FQs) are ubiquitous and ionisable in surface waters. Here we investigate gatifloxacin (GAT) and balofloxacin (BAL), two widely used FQs, and determine the photochemical reactivity of their respective dissociation species that arise at different pH to understand the relevance and pathways of phototransformation reactions. Simulated-sunlight experiments and matrix calculations showed that neutral forms (HFQs0) of the two antibiotics had the highest apparent photolytic efficiency and hydroxyl-radical oxidation reactivity. Based on the pH-dependent photochemical reactivities, the solar apparent photodegradation half-lives (t1/2) in sunlit surface waters ranged from 14.5–169 min and was 1–2 orders of magnitude faster than hydroxyl-radical induced oxidation (t1/2 = 20.9–29.8 h). The corresponding pathways were proposed based on the identification of key intermediates using HPLC-ESI-MS/MS. The apparent photodegradation induced defluorination, decarboxylation, and piperazinyl oxidation and rearrangement, whereas hydroxyl-radical oxidation caused hydroxylated defluorination and piperazinyl hydroxylation. The photomodified toxicity of GAT and BAL was examined using an Escherichia coli activity assay. E. coli activity was not affected by BAL, but was significantly affected by the photo-modified solutions of GAT, indicating that primary photo-degradates have a comparable or higher antibacterial activity than the parent GAT. In fresh water and seawater this antibacterial activity remained high for up to 24 h, even after GAT had undergone significant photodegradation (>1 half-life), indicating the potential impact of this chemical on microbial communities in aquatic systems.
- Published
- 2018
34. Aqueous multivariate phototransformation kinetics of dissociated tetracycline:implications for the photochemical fate in surface waters
- Author
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Ge, Linke, Dong, Qianqian, Halsall, Crispin James, Chen, Chang-Er, Li, Jun, Wang, Degao, Zhang, Peng, Yao, Ziwei, Ge, Linke, Dong, Qianqian, Halsall, Crispin James, Chen, Chang-Er, Li, Jun, Wang, Degao, Zhang, Peng, and Yao, Ziwei
- Abstract
Antibiotics are ubiquitous pollutants in aquatic systems and can exist as different dissociated species depending on the water pH. New knowledge of their multivariate photochemical behavior (i.e., the photobehavior of different ionized forms) is needed to improve our understanding on the fate and possible remediation of these pharmaceuticals in surface and waste waters. In this study, the photochemical degradation of aqueous tetracycline (TC) and its dissociated forms (TCH20, TCH−, and TC2−) was investigated. Simulated sunlight experiments and matrix calculations indicated that the three dissociated species had dissimilar photolytic kinetics and photooxidation reactivities. TC2− photodegraded the fastest due to apparent photolysis with a kinetic constant of 0.938 ± 0.021 min−1, followed by TCH− (0.020 ± 0.005 min−1) and TCH20 (0.012 ± 0.001 min−1), whereas TCH− was found to be the most highly reactive toward •OH (105.78 ± 3.40 M−1s−1), and TC2− reacted the fastest with 1O2 (344.96 ± 45.07 M−1 s−1). Water with relatively high pH (e.g., ~ 8–9) favors the dissociated forms of TCH− and TC2− which are most susceptible to photochemical loss processes compared to neutral TC. The calculated corresponding environmental half-lives (t1/2,E) in sunlit surface waters ranged from 0.05 h for pH = 9 in midsummer to 3.68 h for pH = 6 in midwinter at 45° N latitude. The process was dominated by apparent photolysis (especially in summer, 62–91%), followed by 1O2 and •OH oxidation. Adjusting the pH to slightly alkaline conditions prior to UVor solar UV light treatment may be an effective way of enhancing the photochemical removal of TC from contaminated water.
- Published
- 2018
35. Polychlorinated biphenyls (PCBs) as sentinels for the elucidation of Arctic environmental change processes:a comprehensive review combined with ArcRisk project results
- Author
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Carlsson, Pernilla, Breivik, Knut, Brorström-Lundén, Eva, Cousins, Ian, Christensen, Jesper, Grimalt, Joan O., Halsall, Crispin James, Kallenborn, Roland, Abass, Khaled, Lammel, Gerhard, Munthe, John, MacLeod, Matthew, Øyvind Odland, Jon, Pawlak, Janet, Rautio, Arja, Reiersen, Lars-Otto, Schlabach, Martin, Stemmler, Irene, Wilson, Simon, Wöhrnschimmel, Henry, Carlsson, Pernilla, Breivik, Knut, Brorström-Lundén, Eva, Cousins, Ian, Christensen, Jesper, Grimalt, Joan O., Halsall, Crispin James, Kallenborn, Roland, Abass, Khaled, Lammel, Gerhard, Munthe, John, MacLeod, Matthew, Øyvind Odland, Jon, Pawlak, Janet, Rautio, Arja, Reiersen, Lars-Otto, Schlabach, Martin, Stemmler, Irene, Wilson, Simon, and Wöhrnschimmel, Henry
- Abstract
Polychlorinated biphenyls (PCBs) can be used as chemical sentinels for the assessment of anthropogenic influences on Arctic environmental change. We present an overview of studies on PCBs in the Arctic and combine these with the findings from ArcRisk—a major European Union-funded project aimed at examining the effects of climate change on the transport of contaminants to and their behaviour of in the Arctic—to provide a case study on the behaviour and impact of PCBs over time in the Arctic. PCBs in the Arctic have shown declining trends in the environment over the last few decades. Atmospheric long-range transport from secondary and primary sources is the major input of PCBs to the Arctic region. Modelling of the atmospheric PCB composition and behaviour showed some increases in environmental concentrations in a warmer Arctic, but the general decline in PCB levels is still the most prominent feature. ‘Within-Arctic’ processing of PCBs will be affected by climate change-related processes such as changing wet deposition. These in turn will influence biological exposure and uptake of PCBs. The pan-Arctic rivers draining large Arctic/sub-Arctic catchments provide a significant source of PCBs to the Arctic Ocean, although changes in hydrology/sediment transport combined with a changing marine environment remain areas of uncertainty with regard to PCB fate. Indirect effects of climate change on human exposure, such as a changing diet will influence and possibly reduce PCB exposure for indigenous peoples. Body burdens of PCBs have declined since the 1980s and are predicted to decline further.
- Published
- 2018
36. Exploring the aquatic photodegradation of two ionisable fluoroquinolone antibiotics – Gatifloxacin and balofloxacin : Degradation kinetics, photobyproducts and risk to the aquatic environment
- Author
-
Ge, Linke, Halsall, Crispin, Chen, Chang-Er, Zhang, Peng, Dong, Qianqian, Yao, Ziwei, Ge, Linke, Halsall, Crispin, Chen, Chang-Er, Zhang, Peng, Dong, Qianqian, and Yao, Ziwei
- Abstract
Fluoroquinolone antibiotics (FQs) are ubiquitous and ionisable in surface waters. Here we investigate gatifloxacin (GAT) and balofloxacin (BAL), two widely used FQs, and determine the photochemical reactivity of their respective dissociation species that arise at different pH to understand the relevance and pathways of phototransformation reactions. Simulated-sunlight experiments and matrix calculations showed that neutral forms (HFQs0) of the two antibiotics had the highest apparent photolytic efficiency and hydroxyl-radical oxidation reactivity. Based on the pH-dependent photochemical reactivities, the solar apparent photodegradation half-lives (t1/2) in sunlit surface waters ranged from 14.5–169 min and was 1–2 orders of magnitude faster than hydroxyl-radical induced oxidation (t1/2 = 20.9–29.8 h). The corresponding pathways were proposed based on the identification of key intermediates using HPLC-ESI-MS/MS. The apparent photodegradation induced defluorination, decarboxylation, and piperazinyl oxidation and rearrangement, whereas hydroxyl-radical oxidation caused hydroxylated defluorination and piperazinyl hydroxylation. The photomodified toxicity of GAT and BAL was examined using an Escherichia coli activity assay. E. coli activity was not affected by BAL, but was significantly affected by the photo-modified solutions of GAT, indicating that primary photo-degradates have a comparable or higher antibacterial activity than the parent GAT. In fresh water and seawater this antibacterial activity remained high for up to 24 h, even after GAT had undergone significant photodegradation (>1 half-life), indicating the potential impact of this chemical on microbial communities in aquatic systems.
- Published
- 2018
37. Subtle effects of environmental stress observed in the early life stages of the Common frog, Rana temporaria
- Author
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Strong, Becky, Martin, Francis Luke, Jones, Kevin Christopher, Shore, Richard Francis, and Halsall, Crispin James
- Subjects
fungi - Abstract
Worldwide amphibian populations are declining due to habitat loss, disease and pollution. Vulnerability to environmental contaminants such as pesticides will be dependent on the species, the sensitivity of the ontogenic life stage and hence the timing of exposure and the exposure pathway. Herein we investigated the biochemical tissue ‘fingerprint’ in spawn and early-stage tadpoles of the Common frog, Rana temporaria, using attenuated total reflection-Fourier-transform infrared (ATR-FTIR) spectroscopy with the objective of observing differences in the biochemical constituents of the respective amphibian tissues due to varying water quality in urban and agricultural ponds. Our results demonstrate that levels of stress (marked by biochemical constituents such as glycogen that are involved in compensatory metabolic mechanisms) can be observed in tadpoles present in the pond most impacted by pollution (nutrients and pesticides), but large annual variability masked any inter-site differences in the frog spawn. ATR-FTIR spectroscopy is capable of detecting differences in tadpoles that are present in selected ponds with different levels of environmental perturbation and thus serves as a rapid and cost effective tool in assessing stress-related effects of pollution in a vulnerable class of organism.
- Published
- 2017
38. Subtle effects of environmental stress observed in the early life stages of the common frog, Rana temporaria
- Author
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Strong, Rebecca, Martin, Francis L., Jones, Kevin C., Shore, Richard F., and Halsall, Crispin J.
- Subjects
Zygote ,Rana temporaria ,fungi ,C187 ,C720 ,Lipids ,Article ,Amphibian Proteins ,Ecology and Environment ,Larva ,Water Quality ,Spectroscopy, Fourier Transform Infrared ,Animals ,Pesticides ,Ponds ,Zoology ,Glycogen ,Water Pollutants, Chemical - Abstract
Worldwide amphibian populations are declining due to habitat loss, disease and pollution. Vulnerability to environmental contaminants such as pesticides will be dependent on the species, the sensitivity of the ontogenic life stage and hence the timing of exposure and the exposure pathway. Herein we investigated the biochemical tissue ‘fingerprint’ in spawn and early-stage tadpoles of the Common frog, Rana temporaria, using attenuated total reflection- Fourier-transform infrared (ATR-FTIR) spectroscopy with the objective of observing differences in the biochemical constituents of the respective amphibian tissues due to varying water quality in urban and agricultural ponds. Our results demonstrate that levels of stress (marked by biochemical constituents such as glycogen that are involved in compensatory metabolic mechanisms) can be observed in tadpoles present in the pond most impacted by pollution (nutrients and pesticides), but large annual variability masked any inter-site differences in the frog spawn. ATR-FTIR spectroscopy is capable of detecting differences in tadpoles that are present in selected ponds with different levels of environmental perturbation and thus serves as a rapid and cost effective tool in assessing stress-related effects of pollution in a vulnerable class of organism.
- Published
- 2017
- Full Text
- View/download PDF
39. Pesticides contaminated dust exposure, risk diagnosis and exposure markers in occupational and residential settings of Lahore, Pakistan
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Waheed, Sidra, primary, Halsall, Crispin, additional, Sweetman, Andrew J., additional, Jones, Kevin C., additional, and Malik, Riffat Naseem, additional
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- 2017
- Full Text
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40. Pesticides contaminated dust exposure, risk diagnosis and exposure markers in occupational and residential settings of Lahore, Pakistan
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Waheed, Sidra, Halsall, Crispin, Sweetman, Andrew J., Jones, Kevin C., Malik, Riffat Naseem, Waheed, Sidra, Halsall, Crispin, Sweetman, Andrew J., Jones, Kevin C., and Malik, Riffat Naseem
- Abstract
There are few studies documenting the dust loaded with pesticides as a potential non-dietary exposure source for occupational worker and populations living near agricultural farms and pesticides formulation plants. In present study we have evaluated the pesticide concentration in dust from potential sites and relevant health risk from dust ingestion. Furthermore, the effect of currently used pesticides was investigated on blood and urine parameters of subjects: farmer, factory worker, urban resident and rural resident and controlled subjects with presumably different levels of exposure. The urinary metabolites (TCPY and IMPY) were quantified as biomarkers of exposure to chlorpyrifos and diazinon in relation with biomarkers of effect including BuChE, LH, FSH, testosterone and oxidative stress. Results showed that chlorpyrifos and diazinon were present in higher concentration in dust and posed a high health risk to exposed subjects. The mean SOD value was high among the farmer (3048 U/g Hb) followed by factory worker (1677.6U/g Hb). The urinary biomarkers – TCPY and IMPY- were found higher in exposed subjects as compared to control. Furthermore, testosterone was found in higher concentration in factory worker than control (12.63 ng/ml vs 4.61 ng/ml respectively). A decreased BuChE activity was noticed in occupational group and significant differences were observed in control verses exposed subjects. The PCA analysis evidenced the impact of pesticides on exposure biomarkers and male reproductive hormones. The study suggests that dust contaminated with pesticides engenders significant health risk particularly related to the nervous and endocrine system, not only for occupational workers exposed to direct ingestion but also for nearby residential community. Succinctly putting: Pesticides loaded dust in the city of Lahore, being a high priority concern for the government of Pakistan, demands to be addressed.
- Published
- 2017
41. Polycyclic aromatic hydrocarbons in ocean sediments from the North Pacific to the Arctic Ocean
- Author
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Ma, Yuxin, primary, Halsall, Crispin J., additional, Xie, Zhiyong, additional, Koetke, Danijela, additional, Mi, Wenying, additional, Ebinghaus, Ralf, additional, and Gao, Guoping, additional
- Published
- 2017
- Full Text
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42. Implementing systematic review techniques in chemical risk assessment: Challenges, opportunities and recommendations
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Whaley, Paul, Halsall, Crispin, Ågerstrand, Marlene, Aiassa, Elisa, Benford, Diane, Bilotta, Gary, Coggon, David, Collins, Chris, Dempsey, Ciara, Duarte-Davidson, Raquel, FitzGerald, Rex, Galay-Burgos, Malyka, Gee, David, Hoffmann, Sebastian, Lam, Juleen, Lasserson, Toby, Levy, Len, Lipworth, Steven, Ross, Sarah Mackenzie, Martin, Olwenn, Meads, Catherine, Meyer-Baron, Monika, Miller, James, Pease, Camilla, Rooney, Andrew, Sapiets, Alison, Stewart, Gavin, and Taylor, David
- Subjects
lcsh:GE1-350 ,Research synthesis ,Environment ,Toxicology ,Risk Assessment ,Hazardous Substances ,Article ,Europe ,Review Literature as Topic ,Chemistry ,Environmental Science(all) ,Systematic review ,Animals ,Humans ,Data and Information ,Chemicals ,lcsh:Environmental sciences ,Risk assessment - Abstract
Systematic review (SR) is a rigorous, protocol-driven approach designed to minimise error and bias when summarising the body of research evidence relevant to a specific scientific question. Taking as a comparator the use of SR in synthesising research in healthcare, we argue that SR methods could also pave the way for a "step change" in the transparency, objectivity and communication of chemical risk assessments (CRA) in Europe and elsewhere. We suggest that current controversies around the safety of certain chemicals are partly due to limitations in current CRA procedures which have contributed to ambiguity about the health risks posed by these substances. We present an overview of how SR methods can be applied to the assessment of risks from chemicals, and indicate how challenges in adapting SR methods from healthcare research to the CRA context might be overcome. Regarding the latter, we report the outcomes from a workshop exploring how to increase uptake of SR methods, attended by experts representing a wide range of fields related to chemical toxicology, risk analysis and SR. Priorities which were identified include: the conduct of CRA-focused prototype SRs; the development of a recognised standard of reporting and conduct for SRs in toxicology and CRA; and establishing a network to facilitate research, communication and training in SR methods. We see this paper as a milestone in the creation of a research climate that fosters communication between experts in CRA and SR and facilitates wider uptake of SR methods into CRA. Economic & Social Science Research Council grant “Radical Futures in Social Sciences” (Lancaster University) and Lancaster Environment Centre
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- 2015
43. Implementing systematic review techniques in chemical risk assessment : Challenges, opportunities and recommendations
- Author
-
Whaley, Paul, Halsall, Crispin, Ågerstrand, Marlene, Aiassa, Elisa, Benford, Diane, Bilotta, Gary, Coggon, David, Collins, Chris, Dempsey, Ciara, Duarte-Davidson, Raquel, FitzGerald, Rex, Galay-Burgos, Malyka, Gee, David, Hoffmann, Sebastian, Lam, Juleen, Lasserson, Toby, Levy, Len, Lipworth, Steven, Ross, Sarah Mackenzie, Martin, Olwenn, Meads, Catherine, Meyer-Baron, Monika, Miller, James, Pease, Camilla, Rooney, Andrew, Sapiets, Alison, Stewart, Gavin, Taylor, David, Whaley, Paul, Halsall, Crispin, Ågerstrand, Marlene, Aiassa, Elisa, Benford, Diane, Bilotta, Gary, Coggon, David, Collins, Chris, Dempsey, Ciara, Duarte-Davidson, Raquel, FitzGerald, Rex, Galay-Burgos, Malyka, Gee, David, Hoffmann, Sebastian, Lam, Juleen, Lasserson, Toby, Levy, Len, Lipworth, Steven, Ross, Sarah Mackenzie, Martin, Olwenn, Meads, Catherine, Meyer-Baron, Monika, Miller, James, Pease, Camilla, Rooney, Andrew, Sapiets, Alison, Stewart, Gavin, and Taylor, David
- Abstract
Systematic review (SR) is a rigorous, protocol-driven approach designed to minimise error and bias when summarising the body of research evidence relevant to a specific scientific question. Taking as a comparator the use of SR in synthesising research in healthcare, we argue that SR methods could also pave the way for a step change in the transparency, objectivity and communication of chemical risk assessments (CRA) in Europe and elsewhere. We suggest that current controversies around the safety of certain chemicals are partly due to limitations in current CRA procedures which have contributed to ambiguity about the health risks posed by these substances. We present an overview of how SR methods can be applied to the assessment of risks from chemicals, and indicate how challenges in adapting SR methods from healthcare research to the CRA context might be overcome. Regarding the latter, we report the outcomes from a workshop exploring how to increase uptake of SR methods, attended by experts representing a wide range of fields related to chemical toxicology, risk analysis and SR Priorities which were identified include: the conduct of CRA-focused prototype SRs; the development of a recognised standard of reporting and conduct for SRs in toxicology and CRA; and establishing a network to facilitate research, communication and training in SR methods. We see this paper as a milestone in the creation of a research climate that fosters communication between experts in CRA and SR and facilitates wider uptake of SR methods into CRA.
- Published
- 2016
- Full Text
- View/download PDF
44. Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)
- Author
-
Strong, Becky, Halsall, Crispin James, Ferenčík, Martin, Jones, Kevin Christopher, Shore, Richard Francis, Martin, Francis L, Strong, Becky, Halsall, Crispin James, Ferenčík, Martin, Jones, Kevin Christopher, Shore, Richard Francis, and Martin, Francis L
- Abstract
Amphibians are undergoing large population declines in many regions around the world. As environmental pollution from both agricultural and urban sources has been implicated in such declines, there is a need for a biomonitoring approach to study potential impacts on this vulnerable class of organism. This study assessed the use of infrared (IR) spectroscopy as a tool to detect changes in several tissues (liver, muscle, kidney, heart and skin) of late-stage common frog (Rana temporaria) tadpoles collected from ponds with differing water quality. Small differences in spectral signatures were revealed between a rural agricultural pond and an urban pond receiving wastewater and landfill run-off; these were limited to the liver and heart, although large differences in body size were apparent, surprisingly with tadpoles from the urban site larger than those from the rural site. Large differences in liver spectra were found between tadpoles from the pesticide and nutrient impacted pond compared to the rural agricultural pond, particularly in regions associated with lipids. Liver mass and hepatosomatic indices were found to be significantly increased in tadpoles from the site impacted by pesticides and trace organic chemicals, suggestive of exposure to environmental contamination. Significant alterations were also found in muscle tissue between tadpoles from these two ponds in regions associated with glycogen, potentially indicative of a stress response. This study highlights the use of IR spectroscopy, a low-cost, rapid and reagent-free technique in the biomonitoring of a class of organisms susceptible to environmental degradation.
- Published
- 2016
45. Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides:comparison of single and mixture effects
- Author
-
Strong, Becky, Halsall, Crispin James, Jones, Kevin Christopher, Shore, Richard Francis, Martin, Francis L, Strong, Becky, Halsall, Crispin James, Jones, Kevin Christopher, Shore, Richard Francis, and Martin, Francis L
- Abstract
Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.
- Published
- 2016
46. Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides: comparison of single and mixture effects
- Author
-
Strong, Rebecca J., Halsall, Crispin J., Jones, Kevin C., Shore, Richard F., Martin, Francis L., Strong, Rebecca J., Halsall, Crispin J., Jones, Kevin C., Shore, Richard F., and Martin, Francis L.
- Abstract
Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fouriertransform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.
- Published
- 2016
47. Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)
- Author
-
Strong, Rebecca J., Halsall, Crispin J., Ferenčík, Martin, Jones, Kevin C., Shore, Richard F., Martin, Francis L., Strong, Rebecca J., Halsall, Crispin J., Ferenčík, Martin, Jones, Kevin C., Shore, Richard F., and Martin, Francis L.
- Abstract
Amphibians are undergoing large population declines in many regions around the world. As environmental pollution from both agricultural and urban sources has been implicated in such declines, there is a need for a biomonitoring approach to study potential impacts on this vulnerable class of organism. This study assessed the use of infrared (IR) spectroscopy as a tool to detect changes in several tissues (liver, muscle, kidney, heart and skin) of late-stage common frog (Rana temporaria) tadpoles collected from ponds with differing water quality. Small differences in spectral signatures were revealed between a rural agricultural pond and an urban pond receiving wastewater and landfill run-off; these were limited to the liver and heart, although large differences in body size were apparent, surprisingly with tadpoles from the urban site larger than those from the rural site. Large differences in liver spectra were found between tadpoles from the pesticide and nutrient impacted pond compared to the rural agricultural pond, particularly in regions associated with lipids. Liver mass and hepatosomatic indices were found to be significantly increased in tadpoles from the site impacted by pesticides and trace organic chemicals, suggestive of exposure to environmental contamination. Significant alterations were also found in muscle tissue between tadpoles from these two ponds in regions associated with glycogen, potentially indicative of a stress response. This study highlights the use of IR spectroscopy, a low-cost, rapid and reagent-free technique in the biomonitoring of a class of organisms susceptible to environmental degradation.
- Published
- 2016
48. Assuring high-quality evidence reviews for chemical risk assessment:five lessons from guest editing the first environmental health journal special issue dedicated to systematic review
- Author
-
Whaley, Paul, Halsall, Crispin James, Whaley, Paul, and Halsall, Crispin James
- Published
- 2016
49. Implementing systematic review techniques in chemical risk assessment:challenges, opportunities and recommendations
- Author
-
Whaley, Paul, Halsall, Crispin James, Ågerstrand, Marlene, Aiassa, Elisa, Benford, Diane, Bilotta, Gary S., Coggon, David, Collins, Chris, Dempsey, Ciara, Duarte-Davidson, Raquel, Fitzgerald, Rex, Galay-Burgos, Malyka, Gee, David, Hoffmann, Sebastian, Lam, Juleen, Lasserson, Toby J., Levy, Len, Lipworth, Steven, Mackenzie Ross, Sarah, Martin, Olwenn, Meads, Catherine, Meyer-Baron, Monika, Miller, James, Pease, Camilla, Rooney, Andrew, Sapiets, Alison, Stewart, Gavin, Taylor, David, Whaley, Paul, Halsall, Crispin James, Ågerstrand, Marlene, Aiassa, Elisa, Benford, Diane, Bilotta, Gary S., Coggon, David, Collins, Chris, Dempsey, Ciara, Duarte-Davidson, Raquel, Fitzgerald, Rex, Galay-Burgos, Malyka, Gee, David, Hoffmann, Sebastian, Lam, Juleen, Lasserson, Toby J., Levy, Len, Lipworth, Steven, Mackenzie Ross, Sarah, Martin, Olwenn, Meads, Catherine, Meyer-Baron, Monika, Miller, James, Pease, Camilla, Rooney, Andrew, Sapiets, Alison, Stewart, Gavin, and Taylor, David
- Abstract
Systematic review (SR) is a rigorous, protocol-driven approach designed to minimise error and bias when summarising the body of research evidence relevant to a specific scientific question. Taking as a comparator the use of SR in synthesising research in healthcare, we argue that SR methods could also pave the way for a “step change” in the transparency, objectivity and communication of chemical risk assessments (CRA) in Europe and elsewhere. We suggest that current controversies around the safety of certain chemicals are partly due to limitations in current CRA procedures which have contributed to ambiguity about the health risks posed by these substances. We present an overview of how SR methods can be applied to the assessment of risks from chemicals, and indicate how challenges in adapting SR methods from healthcare research to the CRA context might be overcome. Regarding the latter, we report the outcomes from a workshop exploring how to increase uptake of SR methods, attended by experts representing a wide range of fields related to chemical toxicology, risk analysis and SR. Priorities which were identified include: the conduct of CRA-focused prototype SRs; the development of a recognised standard of reporting and conduct for SRs in toxicology and CRA; and establishing a network to facilitate research, communication and training in SR methods. We see this paper as a milestone in the creation of a research climate that fosters communication between experts in CRA and SR and facilitates wider uptake of SR methods into CRA.
- Published
- 2016
50. Infrared spectroscopy detects changes in an amphibian cell line induced by fungicides : comparison of single and mixture effects
- Author
-
Strong, Becky, Halsall, Crispin James, Jones, Kevin Christopher, Shore, Richard Francis, Martin, Francis Luke, Strong, Becky, Halsall, Crispin James, Jones, Kevin Christopher, Shore, Richard Francis, and Martin, Francis Luke
- Abstract
Amphibians are regarded as sensitive sentinels of environmental pollution due to their permeable skin and complex life cycle, which usually involves reproduction and development in the aquatic environment. Fungicides are widely applied agrochemicals and have been associated with developmental defects in amphibians; thus, it is important to determine chronic effects of environmentally-relevant concentrations of such contaminants in target cells. Infrared (IR) spectroscopy has been employed to signature the biological effects of environmental contaminants through extracting key features in IR spectra with chemometric methods. Herein, the Xenopus laevis (A6) cell line was exposed to low concentrations of carbendazim (a benzimidazole fungicide) or flusilazole (a triazole fungicide) either singly or as a binary mixture. Cells were then examined using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy coupled with multivariate analysis. Results indicate significant changes in the IR spectra of cells induced by both agents at all concentrations following single exposures, primarily in regions associated with protein and phospholipids. Distinct differences were apparent in the IR spectra of cells exposed to carbendazim and those exposed to flusilazole, suggesting different mechanisms of action. Exposure to binary mixtures of carbendazim and flusilazole also induced significant spectral alterations, again in regions associated with phospholipids and proteins, but also in regions associated with DNA and carbohydrates. Overall these findings demonstrate that IR spectroscopy is a sensitive technique for examining the effects of environmentally-relevant levels of fungicides at the cellular level. The combination of IR spectroscopy with the A6 cell line could serve as a useful model to identify agents that might threaten amphibian health in a rapid and high throughput manner.
- Published
- 2016
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