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Background Left ventricular remodeling (LVR) in patients with ST-segment elevation myocardial infarction (STEMI) may lead to poor prognosis in which circulating exosome miRNAs play a critical role. The aim of the present study is to identify specific exosome miRNAs for LVR in patients with STEMI. Method Plasma exosome miRNAs were assessed in 20 patients (90% male, mean age of 66.95±1.65 years) 3–6 months after STEMI and 24 healthy individuals (83% male, mean age of 33.2±0.93 years) by using qPCR. Of the 20 patients, 8 had post-STEMI LVR according to echocardiographic evaluation, and the others did not. Clinical biochemical data including total cholesterol, HDL-C, LDL-C, LDH and NT-pro-BNP were collected from the patients with STEMI at same time as exosome miRNAs assessment. Specific exosome miRNAs for LVR were identified by using qPCR. Correlations between the dysregulated exosome miRNAs and the clinical biochemical parameters in patients with STEMI were analyzed using spearman correlation test. Results Five exosome miRNAs including hsa-miR-181a-3p (p Conclusions Circulating exosome miR-181a-3p and let-7d-3p might play a potential role in LVR in patients 3–6 months after STEMI. Exosome hsa-miR-874-3p might be associated with cardiomyocyte injury. Hsa-miR-23a-3p and hsa-miR-224-5p demonstrated an activity in regulation of lipid metabolism and biosynthesis in patients with STEMI. Funding Acknowledgement Type of funding sources: Public hospital(s). Main funding source(s): This work was supported by grants from the 3×3 Clinical Scientist Fund of Sun Yat-sen Memorial Hospital

循环肿瘤DNA（ctDNA）是液体活检代表性指标之一，近年来在肿瘤诊治中的价值备受关注。ctDNA临床研究涉及的方向包括伴随诊断、疗效预测、预后判断、耐药监测、免疫治疗等。随着相关临床研究的开展，ctDNA逐渐从基础走向临床应用。本文回顾近期ctDNA临床研究进展，介绍新的观点及研究思路，探讨ctDNA临床研究瓶颈及前景。.

An association between Candida and oral cancer has long been discussed in the literature and in particular Candida albicans has been proposed to play a significant role in the development of oral cancer. Although this organism is a common member of the oral mucosa and can cause a variety of oral mucosal lesions, the exact mechanisms by which C. albicans potentially causes the development of malignant disease still require much research. We have undertaken an extensive literature search to understand pathogenicity of C. albicans, including its virulence factors, its interactions with the host immune, how a dysregulation of the immune response can result in malignancy, and how a potential C. albicans specific cytokine response may be involved in oral carcinogenesis. We present here a candid assessment of the role of Candida in oral carcinogenesis and a hypothetical model about how this may occur. Finally, we present potential future research which is necessary to elucidate this role.

The ultrarapid underpass (URUP) tunneling method, in which a shield machine is launched and received at the ground surface level, has been developed for tunneling in congested areas. This p...

To study the characteristics of blood lipids levels' change during pregnancy, and to discuss the relationships between the blood lipid level and the age, gestational hypertension, gestational diabetes and other pregnancy complications.From January to September 2015, we enrolled 2 907 pregnant women admitted to our hospital for regular checks as control group, 190 cases in gestational hypertension group, 258 cases in gestational diabetes mellitus group. The control group was divided into first trimester (≤12 w) second trimester (20 to 26 w) and third trimester (≥28 w) groups; in second trimester group, patients were further divided into the certain age group (35years old) and elderly group (≥35 years old); blood lipid levels including serum levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) were detected in all groups.(1) Blood lipids levels varied as pregnancy proceeded. TG level increased most significantly, followed by TC, LDL-C level. However, HDL-C level slightly decreased in third trimester comparing with first and second trimester stage, the difference was statistically significant (P0.05); (2) serum TC, TG and LDL-C level in second trimester were significantly higher in the certain age group than in the elder group (P0.05); (3) in gestational diabetes mellitus group, serum TC, TG, LDL-C levels were significantly higher in first and second trimester than those in the control group (P0.05); (4) in gestational hypertension group, serum TC, TG, LDL-C levels were significantly higher in third trimester than those in the control group (P0.05); HDL-C level was lower in the gestational hypertension group than in the control group, and there was statistically significant difference (P0.05).The blood lipid levels in pregnancy increase gradually as the gestational weeks increase, which can be explained as a normal physiological phenomenon. Elder pregnancy, gestational hypertension and gestational diabetes mellitus patients usually have obvious blood lipid disorder, therefore, it is very important to monitor the blood lipid level during pregnancy stages for predicting and early intervention of pregnancy complications such as gestational diabetes mellitus and gestational hypertension.

To evaluate the hemodynamics of maxillary artery (MA), superficial temporal artery (STA) and ophthalmic artery (OA), and evaluate the hemodynamics prediction capability of these arteries formed compensatory arteries into intracranial using Doppler ultrasonography.The evaluation of MA, STA, and OA with transcranial doppler ultrasonography and with cerebral angiography was made in 106 MMD patients (total of 212 hemispheres of the brain), 58 male and 48 female, aged 39.3±12.0 years old. Doppler ultrasonography measured the following blood flow parameters: peak systolic velocity (PSV), end-diastolic velocity (EDV), and resistance index (RI). DSA evaluate whether MA, STA, OA formed compensatory arteries into intracranial. Based on the compensation situation, the patients were divided into two groups: compensatory group and non-compensatory group. The differences of patient's hemodynamic parameters between compensatory and non-compensatory groups were performed using independent two-sample t-tests with equal or non-equal variance as appropriate. Categorical variables were summarized using frequency and percentage and compared using Chi-square tests. We evaluated the prediction ability of each hemodynamic parameters for each artery (combining left and right side) using Receiver Operating Curve. All the analyses were performed using SAS 9.4 (Cary, NC).Comparing the hemodynamic parameters between the compensatory group and non-compensatory group, all hemodynamic parameters of MA, STA and OA have statistically significant differences between the two groups. Depending on the ROC Curve, EDV (AUC=0.6933±0.0463) for MA, RI (AUC=0.8910±0.0569) for STA, EDV (AUC=0.7863± 0.0330) for OA are better predictors of compensatory growth.Color duplex ultrasonography is a reliable, noninvasive and economic tool to assess hemodynamic changes of MA, STA and OA, and has prediction capability of these arteries formed compensatory arteries into intracranial.

High epitaxial quality BaTiO3 films were deposited on the MgO (001) substrate using RF magnetron sputtering at 800 °C by manipulating processing parameters. The BaTiO3 films have a ~200 nm thickness with a very low surface roughness but a rough interface structure with respect to the substrate. The epitaxial BaTiO3 films have a tetragonal crystal structure (a = 4.02 A and c = 4.11 A) with a tetragonality (c/a) of 1.02. The c-axis of the film is parallel to the growth direction as characterized by X-ray diffraction, electron diffraction, and high-resolution transmission electron microscopy. The orientation relationship between the film and the MgO is (001)BTO//(001)MgO and 〈100〉BTO//〈100〉MgO. Epitaxial nanodomains were formed in the film with a size ranging from 3 to 20 nm. The formation of the nanodomains is associated with the rough film/substrate interface due to the modification of the substrate surface characteristics (steps, terraces, and kinks) during the process. The two-dimensional interface structure between the film and the substrate was studied and its influence on the film microstructure is discussed.

Cell-free yeast extract has been successfully used to catalyze the enzymatic formation of 11 out of the 14 naturally occurring modified nucleotides in yeast tRNAPhe(anticodon GAA). They are m2G10, D17, m22G26, Cm32, Gm34,psi39, m5C40, m7G46, m5C49, T54 andpsi55. Only D16, Y37 and m1A58 were not formed under in vitro conditions. However, m1G37was quantitatively produced instead of Y37. The naturally occurring intron was absolutely required for m5C40formation while it hindered completely the enzymatic formation of Cm32, Gm34and m1G37. Enzymatic formation of m22G26,psi39, m7G46, m5C49, T54 andpsi55were not or only slightly affected by the presence of the intron. These results allow us to classify the different tRNA modification enzymes into three groups: intron insensitive, intron dependent, and those requiring the absence of the intron. The fact that truncated tRNAPheconsisting of the anticodon stem and loop prolonged with the 19 nucleotide long intron is a substrate for tRNA: cytosine-40 methylase demonstrates that the enzyme is not only strictly intron dependent, but also does not require fully structured tRNA.

Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-beta-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) and human malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with the relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues.