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T cell trafficking at vascular sites has emerged as a key step in antitumor immunity. Chemokines are credited with guiding the multistep recruitment of CD8+ T cells across tumor vessels. However, the multiplicity of chemokines within tumors has obscured the contributions of individual chemokine receptor/chemokine pairs to this process. Moreover, recent studies have challenged whether T cells require chemokine receptor signaling at effector sites. Here, we investigate the hierarchy of chemokine receptor requirements during T cell trafficking to murine and human melanoma. These studies reveal a non-redundant role for GαI-coupled CXCR3 in stabilizing intravascular adhesion and extravasation of adoptively transferred CD8+ effectors that is indispensable for therapeutic efficacy. In contrast, functional CCR2 and CCR5 on CD8+ effectors fail to support trafficking despite the presence of intratumoral cognate chemokines. Taken together, these studies identify CXCR3-mediated trafficking at the tumor vascular interface as a critical checkpoint to effective T cell-based cancer immunotherapy.

The absorption spectrum of the amyloid beta 1-40 peptide (Abeta(1-40)) conjugated to gold colloidal suspension of 15, 20, 30, and 40 nm size were examined under temperature ranging from 5 to 50 degrees C. As the pH was externally altered repetitively between pH 4 and 10, Abeta(1-40)-coated 20 nm gold colloid nanoparticles exhibited a reversible color change at the entire temperature range tested in this study except for 5 +/- 0.2 degrees C. This reversible change may be due to the fact that hydrophilic Abeta(1-40) evolves between a three-dimensional network containing mainly beta-sheet and alpha-helices, and an intermediate of this process implies a reversible step reported as initiation of the fibrillogenesis in Alzheimer's disease. When other nanosize particles were investigated, Abeta(1-40)-coated 30 and 40 nm colloids exhibited the reversible color change when temperature was lowered to 18 +/- 0.2 and 6 +/- 0.2 degrees C, respectively. This specific and unique size and temperature dependence in reversible color change strongly suggests that the noncovalent intrinsic intermolecular potential formed between the nanocolloidal surface and each Abeta(1-40) monomer conjugated at the surface drives the process.