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“Medusaviridae” is a proposed family of large double-stranded DNA (dsDNA) viruses so far represented by a sole virus isolated from a hot spring. In the present study, we report the isolation and genome sequencing of a second member of this family, medusavirus stheno, discovered from a freshwater sample with an Acanthamoeba castellanii coculture.

Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENV detection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015–2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms., NIH (Grants AI-100190, 1R01-HG009761, 1R01-MH110049, and 1DP1-HL141201)

This study provides an ex-ante impact assessment of the possible modernisation of the EU-Chile Association Agreement. Since the current Agreement entered into force in 2003, Chilean exports of agricultural/food products and services to the EU have nearly tripled while EU exports to Chile have doubled in most sub-sectors. Going forward, simulation results provided by the European Commission shows potential growth in bilateral exports with the EU, especially for goods belonging to manufacturing sub-sectors, with modest positive effects on the macroeconomic variables in both partners. This includes increased opportunities for small and medium sized enterprises and general consumer welfare gains. Trade redistribution may also occur among the main trading partners of the EU and Chile. Furthermore, there are decided advantages to a single EU-wide comprehensive investment chapter to facilitate foreign direct investment flows, while gains from easing market access and regulatory barriers to public procurement in both Chile and the EU are more muted. Overall, limited environmental, employment and labour market impacts are expected in both partners. However, potential exists for reinforcing labour standards in Chile and increased environmental cooperation through a modernised Agreement. The net impact of increased trade on human rights is unclear but likely to be marginal.

Although the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects have attracted a great deal of attention1,2, much remains unknown about ZIKV disease epidemiology and ZIKV evolution, in part owing to a lack of genomic data. Here we address this gap in knowledge by using multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analysed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental United States. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of surveillance of viral infections. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those that might be relevant to the effectiveness of diagnostic tests., National Institutes of Health (U.S.) (Grant NIAID U19AI110818)

This dissertation examines whether the incorporation of labor rights provisions into trade agreements promotes labor rights enforcement in developing countries. It draws on the international relations literature on transnational advocacy as the starting point to ask how labors allies engage the trade mechanisms as potential tools for promoting labor rights in supranational arenas. Using original data, field observation, primary documents and interviews with key participants, I examine how transnational labor rights advocates have engaged these institutions through a research design that pairs quantitative analysis of the NAFTA labor side agreement, with qualitative examination of a number of the NAFTA cases and a set of labor violations cases in Puebla, Mexico. The empirical chapters discuss the ways that transnational labor rights advocates engage the labor rights enforcement mechanism as they attempt to secure a review of their petitions from the quasi-judicial bodies charged with investigating labor rights compliance. Transnational support has led to outcomes for labor that include firm-level redress of labor rights violations and institutional changes within Mexico. The implication for this work is that where labor clauses have had an effect on labor rights practices within states, it has been at the intersection of transnational civil society and international institutions. The research suggests that the process of engaging the petitions mechanism can persuade or coerce states into enforcing labor rights commitments, and emphasizes that transnational advocacy provides a crucial element to realizing labor rights enforcement where domestic efforts are weak.

El presente Informe de Suficiencia Profesional Docente tiene como objetivo evidenciar la adquisición de las 10 competencias docentes que propone la Facultad de Educación de la Pontificia Universidad Católica del Perú según su perfil de egreso en educación primaria y, además, medir el nivel de logro alcanzado por mi persona de dichas competencias en estos 5 años de formación académica. En este sentido, este documento se caracteriza por ser el resultado de una práctica reflexiva que considera las vivencias de la formación recibida en facultad y la práctica pre profesional realizada en el primer y tercer grado de primaria en una escuela pública del distrito de Pueblo Libre. Asimismo, el documento da muestra de la movilización de dichas competencias en las reflexiones que surgen de una experiencia docente realizada específicamente en el tercer grado de primaria de dicha institución. Para concluir, solo destacar que la lectura del informe se complementa con el portafolio virtual, el cual será la herramienta que contiene las evidencias del logro de los desempeños de las competencias previamente mencionadas., The objective of this Teaching Professional Proficiency Report is to demonstrate the acquisition of the 10 teaching competences proposed by the Faculty of Education of the Pontifical Catholic University of Peru according to their progress profile in primary education and, in addition, measure the level of achievement obtained by myself in differents skills in these 5 years of academic training. In this sense, this document is characterized by being the result of a reflective practice that considers the experiences of the training received in faculty and the preprofessional practice carried out in the first and third grade of primary school in a public school in the district of Pueblo Libre. Likewise, the document shows the mobilization of diverse competences in the reflections that arise from a teaching experience carried out specifically in the third grade of said institution. To conclude, just note that the reading of the report is complemented by the virtual portfolio, which will be the tool that contains the evidence of the achievement of the performance of the previously determined competencies.

El presente Informe de Suficiencia Profesional Docente tiene como objetivo evidenciar la adquisición de las 10 competencias docentes que propone la Facultad de Educación de la Pontificia Universidad Católica del Perú según su perfil de egreso en educación primaria y, además, medir el nivel de logro alcanzado por mi persona de dichas competencias en estos 5 años de formación académica. En este sentido, este documento se caracteriza por ser el resultado de una práctica reflexiva que considera las vivencias de la formación recibida en facultad y la práctica pre profesional realizada en el primer y tercer grado de primaria en una escuela pública del distrito de Pueblo Libre. Asimismo, el documento da muestra de la movilización de dichas competencias en las reflexiones que surgen de una experiencia docente realizada específicamente en el tercer grado de primaria de dicha institución. Para concluir, solo destacar que la lectura del informe se complementa con el portafolio virtual, el cual será la herramienta que contiene las evidencias del logro de los desempeños de las competencias previamente mencionadas., The objective of this Teaching Professional Proficiency Report is to demonstrate the acquisition of the 10 teaching competences proposed by the Faculty of Education of the Pontifical Catholic University of Peru according to their progress profile in primary education and, in addition, measure the level of achievement obtained by myself in differents skills in these 5 years of academic training. In this sense, this document is characterized by being the result of a reflective practice that considers the experiences of the training received in faculty and the preprofessional practice carried out in the first and third grade of primary school in a public school in the district of Pueblo Libre. Likewise, the document shows the mobilization of diverse competences in the reflections that arise from a teaching experience carried out specifically in the third grade of said institution. To conclude, just note that the reading of the report is complemented by the virtual portfolio, which will be the tool that contains the evidence of the achievement of the performance of the previously determined competencies.

El presente Informe de Suficiencia Profesional Docente tiene como objetivo evidenciar la adquisición de las 10 competencias docentes que propone la Facultad de Educación de la Pontificia Universidad Católica del Perú según su perfil de egreso en educación primaria y, además, medir el nivel de logro alcanzado por mi persona de dichas competencias en estos 5 años de formación académica. En este sentido, este documento se caracteriza por ser el resultado de una práctica reflexiva que considera las vivencias de la formación recibida en facultad y la práctica pre profesional realizada en el primer y tercer grado de primaria en una escuela pública del distrito de Pueblo Libre. Asimismo, el documento da muestra de la movilización de dichas competencias en las reflexiones que surgen de una experiencia docente realizada específicamente en el tercer grado de primaria de dicha institución. Para concluir, solo destacar que la lectura del informe se complementa con el portafolio virtual, el cual será la herramienta que contiene las evidencias del logro de los desempeños de las competencias previamente mencionadas., The objective of this Teaching Professional Proficiency Report is to demonstrate the acquisition of the 10 teaching competences proposed by the Faculty of Education of the Pontifical Catholic University of Peru according to their progress profile in primary education and, in addition, measure the level of achievement obtained by myself in differents skills in these 5 years of academic training. In this sense, this document is characterized by being the result of a reflective practice that considers the experiences of the training received in faculty and the preprofessional practice carried out in the first and third grade of primary school in a public school in the district of Pueblo Libre. Likewise, the document shows the mobilization of diverse competences in the reflections that arise from a teaching experience carried out specifically in the third grade of said institution. To conclude, just note that the reading of the report is complemented by the virtual portfolio, which will be the tool that contains the evidence of the achievement of the performance of the previously determined competencies.