5 results on '"Gangwar L"'
Search Results
2. Genetic diversity analysis for various morphological and quality traits in bread wheat (Triticum aestivum L.)
- Author
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Singh, Girnam, Kumar, Pradeep, Kumar, Ravi, Gangwar, L. K., Singh, Girnam, Kumar, Pradeep, Kumar, Ravi, and Gangwar, L. K.
- Abstract
The present investigation was carried out during 2011-12 in a randomized block design (RBD) with 35 diverse wheat genotypes to assess the genetic diversity for various morphological and quality traits. The analysis of variance for grain yield and its contributing components namely days to 50% flowering, days to maturity, productive tillers, plant height, spike length, spikelets pet spike, grains per spikelet, biological yield, harvest index, 1000 grain weight, grain yield and gluten content showed highly significant differences (at <1% level of significance) among the genotypes under present study. High heritability along with high genetic advance and high phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) for grain yield (g), biological yield (g), harvest index (%), spike length (cm) and 1000 grain weight (g) indicated substantial contribution of additive gene action in the expression and thus selection would be effective for genetic improvement of these traits for improving grain yield in wheat. On the basis of multivariate analysis, 35 genotypes were grouped into ‘6’ clusters based on genetic divergence (D2 ) value. The compositions of clusters revealed that the Cluster IV contained the highest number of genotypes (9) followed by Cluster II (8), Cluster VI (8) and Cluster III (7). The highest inter cluster values were recorded between cluster III and V (8357.19) followed by cluster IV and V (7513.88), cluster IV and VI (6009.44) and cluster III and VI (5530.40) exhibiting wide genetic diversity. Among different traits, biological yield (32.12%), productive tillers (28.74%), harvest index (26.71%), plant height (24.20%), grain yield (19.23%) and grains per spikelets (14.89%) had maximum contribution to total genetic divergence, therefore may be used as selection parameters in transgressive segregants. Selection of genotypes from the clusters may be used as potential donors for further hybridization programme to develop genoty
- Published
- 2018
3. De novo transcriptome assembly, annotation and SSR mining data of Hellula undalis (Fabr.) (Lepidoptera: Pyralidae), the cabbage webworm.
- Author
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Prajapati MR, Kumar P, Pratap Singh R, Shanker R, Singh J, Kumar Bharti M, Singh R, Verma H, Gangwar LK, Singh Gaurav S, Kapoor N, Prakash S, and Dixit R
- Abstract
Background: The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for acquiring complete transcriptome information of insect species when there is no reference genome available. In case of Hellula undalis, only 17 nucleotide records are currently available throughout NCBI nucleotide database. Genes associated with metabolic processes, general development, reproduction, defense and functional genomics were not previously predicted in the Hellula undalis at the genomic level., Methods & Results: To address this issue, we constructed Hellula undalis transcriptome using Illumina NovaSeq6000 technology. Approximately 48 million 150 bp paired-end reads were obtained from sequencing. A total of 30,451 contigs were generated by de novo assembly of sample and were compared with the sequences in the NCBI non-redundant protein database (Nr). In total, 71 % of contigs were matched to known proteins in public databases including Nr, Gene Ontology (GO), and Cluster Orthologous Gene Database (COG), and then, contigs were mapped to 123 via functional annotation against the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). In addition, we compared the ortholog gene family of the Hullula undalis, transcriptome to Spodoptera frugiperda, spodotera litura and spodoptera littoralis and found that 391 orthologous gene families are specific to Hullula undalis. A total of 1,913 potential SSRs was discovered in Hullula undalis contigs., Conclusions: This study is the first transcriptome data for Hullula undalis. Additionally, it serves as a valuable resource for identifying target genes and developing effective and environmentally friendly strategies for pest control., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
4. Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model.
- Author
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Han Z, Rao JS, Gangwar L, Namsrai BE, Pasek-Allen JL, Etheridge ML, Wolf SM, Pruett TL, Bischof JC, and Finger EB
- Subjects
- Male, Rats, Animals, Cryopreservation methods, Kidney, Organ Preservation methods, Vitrification, Kidney Transplantation
- Abstract
Banking cryopreserved organs could transform transplantation into a planned procedure that more equitably reaches patients regardless of geographical and time constraints. Previous organ cryopreservation attempts have failed primarily due to ice formation, but a promising alternative is vitrification, or the rapid cooling of organs to a stable, ice-free, glass-like state. However, rewarming of vitrified organs can similarly fail due to ice crystallization if rewarming is too slow or cracking from thermal stress if rewarming is not uniform. Here we use "nanowarming," which employs alternating magnetic fields to heat nanoparticles within the organ vasculature, to achieve both rapid and uniform warming, after which the nanoparticles are removed by perfusion. We show that vitrified kidneys can be cryogenically stored (up to 100 days) and successfully recovered by nanowarming to allow transplantation and restore life-sustaining full renal function in nephrectomized recipients in a male rat model. Scaling this technology may one day enable organ banking for improved transplantation., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
5. Vitrification and Nanowarming of Kidneys.
- Author
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Sharma A, Rao JS, Han Z, Gangwar L, Namsrai B, Gao Z, Ring HL, Magnuson E, Etheridge M, Wowk B, Fahy GM, Garwood M, Finger EB, and Bischof JC
- Subjects
- Animals, Ferric Compounds, Kidney anatomy & histology, Male, Models, Animal, Rats, Rats, Sprague-Dawley, X-Ray Microtomography methods, Cryopreservation methods, Kidney physiology, Nanoparticles, Rewarming methods, Vitrification
- Abstract
Vitrification can dramatically increase the storage of viable biomaterials in the cryogenic state for years. Unfortunately, vitrified systems ≥3 mL like large tissues and organs, cannot currently be rewarmed sufficiently rapidly or uniformly by convective approaches to avoid ice crystallization or cracking failures. A new volumetric rewarming technology entitled "nanowarming" addresses this problem by using radiofrequency excited iron oxide nanoparticles to rewarm vitrified systems rapidly and uniformly. Here, for the first time, successful recovery of a rat kidney from the vitrified state using nanowarming, is shown. First, kidneys are perfused via the renal artery with a cryoprotective cocktail (CPA) and silica-coated iron oxide nanoparticles (sIONPs). After cooling at -40 °C min
-1 in a controlled rate freezer, microcomputed tomography (µCT) imaging is used to verify the distribution of the sIONPs and the vitrified state of the kidneys. By applying a radiofrequency field to excite the distributed sIONPs, the vitrified kidneys are nanowarmed at a mean rate of 63.7 °C min-1 . Experiments and modeling show the avoidance of both ice crystallization and cracking during these processes. Histology and confocal imaging show that nanowarmed kidneys are dramatically better than convective rewarming controls. This work suggests that kidney nanowarming holds tremendous promise for transplantation., (© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH.)- Published
- 2021
- Full Text
- View/download PDF
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