1. Role of glutathione S-transferases polymorphisms and monocyte CD64 expression in Egyptian patients with systemic lupus erythematosus
- Author
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Hanan Darweesh, Hala Lotfy Fayed, Engy El Khateeb, Aml S Nasr, and Al-Hussein M. El-Dakrony
- Subjects
0301 basic medicine ,lcsh:Immunologic diseases. Allergy ,SLE ,Renal function ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,GSTP1 ,0302 clinical medicine ,Monocyte CD64 ,Rheumatology ,GSTP1 polymorphisms ,Genotype ,medicine ,skin and connective tissue diseases ,Gene ,030203 arthritis & rheumatology ,CD64 ,GSTT1 ,SLEDAI ,medicine.diagnostic_test ,business.industry ,Monocyte ,Glutathione ,SLICC damage index ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Immunology ,business ,lcsh:RC581-607 ,GSTM1 - Abstract
Aim of work: To study the genetic variants of glutathione S-transferases and monocyte CD64 expression in systemic lupus erythematosus patients and to evaluate their role in disease susceptibility, activity and damage. Patients and methods: Forty female SLE patients and 40 age matched controls were genotyped for GSTP1, GSTM1 and GSTT1 gene polymorphisms using polymerase chain reaction-restriction fragment length polymorphism, conventional PCR and were assessed for monocyte CD64 expression level using flow cytometry. SLE disease activity index (SLEDAI) and the systemic lupus international. collaborating clinics/damage index (SLICC DI) were considered. Results: The patients mean age was 28.13 ± 4.56 years and disease duration of 6.4 ± 4.9 with a SLEDAI of 14.4 ± 7.1 and SLICC/DI 3.7 ± 1.5. The frequency of GSTM1 null genotype tended to be higher (55%) in SLE patients compared to the controls (and 42.5%) (p = 0.09). The frequency of GSTT1 null genotype was significantly higher in SLE patients (25%) compared to controls (12.5%) (p
- Published
- 2017