103 results on '"Fukatsu K"'
Search Results
2. Measurement of zero degree inclusive photon energy spectra for $\sqrt{s}=$ 900 GeV proton-proton collisions at LHC
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D'Alessandro, R., Fukatsu, K., Haguenauer, M., Iso, T., Itow, Y., Kasahara, K., Kawade, K., Mase, T., Masuda, K., Menjo, H., Mitsuka, G., Muraki, Y., Noda, K., Papini, P., Perrot, A. -L., Ricciarini, S., Sako, T., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, A., and Turner, W. C.
- Subjects
High Energy Physics - Experiment ,Nuclear Experiment - Abstract
The inclusive photon energy spectra measured by the Large Hadron Collider forward (LHCf) experiment in the very forward region of LHC proton-proton collisions at $\sqrt{s}=$ 900 GeV are reported. The results from the analysis of 0.30 $\mathrm{nb^{-1}}$ of data collected in May 2010 in the two pseudorapidity regions of $\eta > 10.15$ and $8.77 < \eta < 9.46$ are compared with the predictions of the hadronic interaction models DPMJET 3.04, EPOS 1.99, PYTHIA 8.145, QGSJET I -.1em I-03 and SIBYLL 2.1, which are widely used in ultra-high-energy cosmic-ray experiments. EPOS 1.99 and SYBILL 2.1 show a reasonable agreement with the spectral shape of the experimental data, whereas they predict lower cross-sections than the data. The other models, DPMJET 3.04, QGSJET I -.1em I-03 and PYTHIA 8.145, are in good agreement with the data below 300 GeV but predict harder energy spectra than the data above 300 GeV. The results of these comparisons exhibited features similar to those for the previously reported data for $\sqrt{s}=$ 7 TeV collisions.
- Published
- 2012
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3. Measurement of forward neutral pion transverse momentum spectra for $\sqrt{s}$ = 7TeV proton-proton collisions at LHC
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D'Alessandro, R., Fukatsu, K., Haguenauer, M., Iso, T., Itow, Y., Kasahara, K., Kawade, K., Mase, T., Masuda, K., Menjo, H., Mitsuka, G., Muraki, Y., Noda, K., Papini, P., Perrot, A. -L., Ricciarini, S., Sako, T., Shimizu, Y., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, A., and Turner, W. C.
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High Energy Physics - Experiment - Abstract
The inclusive production rate of neutral pions in the rapidity range greater than $y=8.9$ has been measured by the Large Hadron Collider forward (LHCf) experiment during LHC $\sqrt{s}=7$\,TeV proton-proton collision operation in early 2010. This paper presents the transverse momentum spectra of the neutral pions. The spectra from two independent LHCf detectors are consistent with each other and serve as a cross check of the data. The transverse momentum spectra are also compared with the predictions of several hadronic interaction models that are often used for high energy particle physics and for modeling ultra-high-energy cosmic-ray showers., Comment: 18 Pages, 10 figures, submitted to Phys. Rev. D
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- 2012
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4. Measurement of zero degree single photon energy spectra for sqrt(s) = 7TeV proton-proton collisions at LHC
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D'Alessandro, R., Faus, A., Fukatsu, K., Haguenauer, M., Itow, Y., Kasahara, K., Kawade, K., Macina, D., Mase, T., Masuda, K., Matsubara, Y., Menjo, H., Mitsuka, G., Muraki, Y., Nakai, M., Noda, K., Papini, P., Perrot, A. -L., Ricciarini, S., Sako, T., Shimizu, Y., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, A., Turner, W. C., Velasco, J., Viciani, A., and Yoshida, K.
- Subjects
High Energy Physics - Experiment - Abstract
In early 2010, the Large Hadron Collider forward (LHCf) experiment measured very forward neutral particle spectra in LHC proton-proton collisions. From a limited data set taken under the best beam conditions (low beam-gas background and low occurance of pile-up events), the single photon spectra at sqrt(s)=7TeV and pseudo-rapidity (eta) ranges from 8.81 to 8.99 and from 10.94 to infinity were obtained for the first time and are reported in this paper. The spectra from two independent LHCf detectors are consistent with one another and serve as a cross check of the data. The photon spectra are also compared with the predictions of several hadron interaction models that are used extensively for modeling ultra high energy cosmic ray showers. Despite conservative estimates for the systematic errors, none of the models agree perfectly with the measurements. A notable difference is found between the data and the DPMJET 3.04 and PYTHIA 8.145 hadron interaction models above 2TeV where the models predict higher photon yield than the data. The QGSJET II-03 model predicts overall lower photon yield than the data, especially above 2TeV in the rapidity range 8.81
- Published
- 2011
- Full Text
- View/download PDF
5. Early results of the LHCf Experiment and their contribution to Ultra-High-Energy Cosmic Ray Physics
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D'Alessandro, R., Faus, A., Fukatsu, K., Haguenauer, M., Itow, Y., Kasahara, K., Macina, D., Mase, T., Masuda, K., Matsubara, Y., Menjo, H., Mitsuka, G., Muraki, Y., Nakai, M., Noda, K., Papini, P., Perrot, A L., Ricciarini, S., Sako, T., Suzuki, K., Suzuki, T., Shimizu, Y., Taki, K., Tamura, T., Torii, S., Tricomi, A., Velasco, J., Turner, W. C., and Yoshida, K.
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High Energy Physics - Experiment - Abstract
LHCf is an experiment dedicated to the measurement of neutral particles emitted in the very forward region of LHC collisions. The physics goal is to provide data for calibrating hadron interaction models that are used in the study of Extremely High-Energy Cosmic-Rays. The LHCf experiment acquired data from April to July 2010 during commissioning time of LHC operations at low luminosity. Production spectra of photons and neutrons emitted in the very forward region ($\eta>$ 8.4) have been obtained. In this paper preliminary results of the photon spectra taken at $\sqrt{s}$ = 7TeV are reported., Comment: This paper has been presented in the international conference CRIS 2010 for 100 years of cosmic ray physics: from pioneering experiments to physics in space. The paper will be published in Nuclear Physics B (conference proceedings)
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- 2010
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6. Highly reliable high-speed 1.1- [mu]m-range VCSELs with InGaAs/GaAsP-MQWs
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Hatakeyama, H., Anan, T., Akagawa, T., Fukatsu, K., Suzuki, N., Tokutome, K., and Tsuji, M.
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Gallium arsenide -- Electric properties ,Gallium arsenide -- Optical properties ,Gallium arsenide -- Thermal properties ,Indium -- Electric properties ,Indium -- Optical properties ,Indium -- Thermal properties ,Semiconductor lasers -- Thermal properties ,Semiconductor lasers -- Electric properties ,Failure mode and effects analysis ,Business ,Computers ,Electronics ,Electronics and electrical industries - Published
- 2010
7. Measurement of zero degree inclusive photon energy spectra for root s=900 GeV proton-proton collisions at LHC
- Author
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Adriani, O, Bonechi, L, Bongi, M, Castellini, G, D'Alessandro, R, Fukatsu, K, Haguenauer, M, Iso, T, Itow, Y, Kasahara, K, Kawade, K, Mase, T, Masuda, K, Menjo, H, Mitsuka, G, Muraki, Y, Noda, K, Papini, P, Perrot, Al, Ricciarini, S, Sako, T, Suzuki, K, Suzuki, T, Taki, K, Tamura, T, Torii, S, Tricomi, Alessia Rita, and Turner, Wc
- Published
- 2012
8. Measurement of forward neutral pion transverse momentum spectra for √s = 7TeV proton-proton collisions at LHC
- Author
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D'Alessandro, R., Fukatsu, K., Haguenauer, M., Iso, T., Itow, Y., Kasahara, K., Kawade, K., Mase, T., Masuda, K., Menjo, H., Mitsuka, G., Muraki, Y., Noda, K., Papini, P., Perrot, A. L., Ricciarini, S., Sako, T., Shimizu, Y., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, Alessia Rita, and Turner, W. C.
- Published
- 2012
9. Measurement of zero degree single photon energy spectra for $\sqrt{s}$ = 7 TeV proton-proton collisions at LHC
- Author
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D'Alessandro, R., Faus, A., Fukatsu, K., Haguenauer, M., Itow, Y., Kasahara, K., Kawade, K., Macina, D., Mase, T., Masuda, K., Matsubara, Y., Menjo, H., Mitsuka, G., Muraki, Y., Nakai, M., Noda, K., Papini, P., Perrot, A. -L., Ricciarini, S., Sako, T., Shimizu, Y., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, A., Turner, W. C., Velasco, J., Viciani, A., and Yoshida, K.
- Subjects
High Energy Physics - Experiment (hep-ex) ,FOS: Physical sciences ,High Energy Physics::Experiment ,Nuclear Experiment ,Particle Physics - Experiment ,High Energy Physics - Experiment - Abstract
In early 2010, the Large Hadron Collider forward (LHCf) experiment measured very forward neutral particle spectra in LHC proton-proton collisions. From a limited data set taken under the best beam conditions (low beam-gas background and low occurance of pile-up events), the single photon spectra at $\sqrt{s}$ = 7 TeV and pseudo-rapidity ($\eta$) ranges from 8.81 to 8.99 and from 10.94 to infinity were obtained for the first time and are reported in this paper. The spectra from two independent LHCf detectors are consistent with one another and serve as a cross check of the data. The photon spectra are also compared with the predictions of several hadron interaction models that are used extensively for modeling ultra high energy cosmic ray showers. Despite conservative estimates for the systematic errors, none of the models agree perfectly with the measurements. A notable difference is found between the data and the DPMTJET 3.04, PYTHIA 8.145, and QGSJET II-03 hadron interaction models above 2 TeV and for $\eta$ > 10.94 where the models predict higher photon yield than the data. The DPMTJET 3.04 and PYTHIA 8.145 models also predict higher photon yield in the rapidity range 8.81 < $\eta$ < 8.99, but the difference in the spectral shape is not as large as for the higher rapidity results. In early 2010, the Large Hadron Collider forward (LHCf) experiment measured very forward neutral particle spectra in LHC proton–proton collisions. From a limited data set taken under the best beam conditions (low beam-gas background and low occurrence of pile-up events), the single photon spectra at s=7 TeV and pseudo-rapidity ( η ) ranges from 8.81 to 8.99 and from 10.94 to infinity were obtained for the first time and are reported in this Letter. The spectra from two independent LHCf detectors are consistent with one another and serve as a cross check of the data. The photon spectra are also compared with the predictions of several hadron interaction models that are used extensively for modeling ultra-high energy cosmic-ray showers. Despite conservative estimates for the systematic errors, none of the models agree perfectly with the measurements. A notable difference is found between the data and the DPMJET 3.04 and PYTHIA 8.145 hadron interaction models above 2 TeV where the models predict higher photon yield than the data. The QGSJET II-03 model predicts overall lower photon yield than the data, especially above 2 TeV in the rapidity range 8.81
- Published
- 2011
10. Measurement of zero degree single photon energy spectra for root s=7 TeV proton-proton collisions at LHC
- Author
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Adriani, O, Bonechi, L, Bongi, M, Castellini, G, D'Alessandro, R, Faus, A, Fukatsu, K, Haguenauer, M, Itow, Y, Kasahara, K, Kawade, K, Macina, D, Mase, T, Masuda, K, Matsubara, Y, Menjo, H, Mitsuka, G, Muraki, Y, Nakai, M, Noda, K, Papini, P, Perrot, Al, Ricciarini, S, Sako, T, Shimizu, Y, Suzuki, K, Suzuki, T, Taki, K, Tamura, T, Torii, S, Tricomi, Alessia Rita, Turner, Wc, Velasco, J, Viciani, A, and Yoshida, K.
- Published
- 2011
11. The status and preliminary results of the LHC forward experiment: LHCf
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Lorenzo Bonechi, Oscar Adriani, Bonechi, L., Massimo Bongi, Castellini, G., Raffaello D'Alessandro, Faus, A., Fukatsu, K., Haguenauer, M., Itow, Y., Kasahara, K., Kawade, K., Macina, D., Mase, T., Masuda, K., Matsubara, Y., Mitsuka, G., Muraki, Y., Nakai, M., Noda, K., Papini, P., Perrot, A. L., Ricciarini, S., Sako, T., Sinatra, G., Suzuki, K., Suzuki, T., Shimizu, Y., Taki, K., Tamura, T., Torii, S., Tricomi, A., Velasco, J., Turner, W. C., and Yoshida, K.
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Physics ,Particle physics ,Large Hadron Collider ,Detectors and Experimental Techniques ,Particle Physics - Experiment - Published
- 2010
12. Early physics results of the LHCf experiment
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Tricomi, A., Adriani, O., Bonechi, L., Bongi, M., Castellini, G., D Alessandro, R., Fukatsu, K., Hagenauer, M., Itow, Y., Kasahara, K., Kawade, K., Macina, D., Mase, T., Masuda, K., Matsubara, Y., Menjo, H., Mitsuka, G., Muraki, Y., Nakai, M., Koji Noda, Papini, P., Perrot, A. L., Ricciarini, S., Sako, T., Suzuki, K., Suzuki, T., Shimizu, Y., Taki, K., Tamura, T., Torii, S., Turner, W. C., and Yoshida, K.
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Particle Physics - Experiment - Published
- 2010
13. Highly Reliable High-Speed 1.1-$\mu$m-Range VCSELs With InGaAs/GaAsP-MQWs
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Hatakeyama, H., primary, Anan, T., additional, Akagawa, T., additional, Fukatsu, K., additional, Suzuki, N., additional, Tokutome, K., additional, and Tsuji, M., additional
- Published
- 2010
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14. Clinicopathological and Prognostic Relevance of Uptake Level using 18F-fluorodeoxyglucose Positron Emission Tomography/Computed Tomography Fusion Imaging (18F-FDG PET/CT) in Primary Breast Cancer
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Ueda, S., primary, Tsuda, H., additional, Asakawa, H., additional, Shigekawa, T., additional, Fukatsu, K., additional, Kondo, N., additional, Yamamoto, M., additional, Hama, Y., additional, Tamura, K., additional, Ishida, J., additional, Abe, Y., additional, and Mochizuki, H., additional
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- 2008
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15. GENDER DIFFERENCES IN CYTOKINE PRODUCTION AND SURFACE ANTIGEN EXPRESSION OF MONOCYTES FOLLOWING GASTROINTESTINAL SURGERY.
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Ono, S., primary, Tsujimoto, H., additional, Hiraki, S., additional, Kimura, A., additional, Kinoshita, M., additional, Fukatsu, K., additional, Hiraide, H., additional, Seki, S., additional, Ichikura, T., additional, and Mochizuki, H., additional
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- 2004
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16. DIFFERENTIAL MECHANISM OF IMMUNE SUPPRESSION IN PATIENTS WITH SEPTIC AND NON-SEPTIC SURGICAL STRESS
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Hiraki, S., primary, Ono, S., additional, Kinoshita, M., additional, Tsuiimoto, H., additional, Kawarabavashi, N., additional, Kimura, A., additional, Fukatsu, K., additional, Hiraide, H., additional, Seki, S., additional, Ichikura, T., additional, and Mochizuki, H., additional
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- 2004
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17. AN ICAM-1 ANTIBODY AND INHIBITORS OF PMN-TOXIC METABOLITES REDUCE PULMONARY PMN ADHESION IN ENDOTOXEMIC RATS -VISUAL QUANTIFICATION BY FLUORESCENT MICROSCOPIC METHOD-
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Han, I., primary, Saito, H., additional, Fukatsu, K., additional, Furukawa, S., additional, Matsuda, T., additional, Inoue, T., additional, Lin, M. T., additional, Yasuhara, H., additional, Ikeda, S., additional, and Muto, T., additional
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- 1997
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18. INGESTION OF SMALL NUMBERS OF BACTERIA PREVENTS PMN CELL DEATH AND INDUCES PMN APOPTOSIS
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Matsuda, T., primary, Saito, H., additional, Tsuno, N., additional, Inoue, T., additional, Lin, M. T., additional, Fukatsu, K., additional, Han, I., additional, Furukawa, S., additional, Ikeda, S., additional, and Muto, T., additional
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- 1997
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19. NITRIC OXIDE DONOR DECREASES PMN ADHESION IN LUNG AND PERITONEUM DURING PERITONITIS -A STUDY BY NEW METHOD FOR VISUALIZATION OF PMN ADHESION-
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Fukatsu, K., primary, Saito, H., additional, Han, I., additional, Furukawa, S., additional, Lin, M T., additional, Inoue, T., additional, Ikeda, S., additional, Matsuda, T., additional, Hashiguchi, Y., additional, Inaba, T., additional, and Muto, T., additional
- Published
- 1997
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20. EFFECTS OF GH AND IGF-I ON OPSONIN RECEPTOR EXPRESSION, PHAGOCYTOSIS AND REACTIVE OXYGEN INTERMEDIATES PRODUCTION BY HUMAN POLYMORPHONUCLEAR NEUTROPHILS.
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Inoue, T., primary, Saito, H., additional, Matsuda, T., additional, Fukatsu, K., additional, Lin, M. T., additional, Han, I., additional, Furukawa, S., additional, Ikeda, S., additional, and Muto, T., additional
- Published
- 1997
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21. Microphysical investigations on mechanical structures realized in p/sup +/ silicon
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Cabuz, C., primary, Fukatsu, K., additional, Kurabayashi, T., additional, Minami, K., additional, and Esashi, M., additional
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- 1995
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22. The 4 Orthogonality Condition Model for Low-Lying 0+ States of 16O
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Fukatsu, K., primary and Kato, K., additional
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- 1992
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23. Measurement of zero degree inclusive photon energy spectra for s=900GeV proton–proton collisions at LHC
- Author
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., DʼAlessandro, R., Fukatsu, K., Haguenauer, M., Iso, T., Itow, Y., Kasahara, K., Kawade, K., Mase, T., Masuda, K., Menjo, H., Mitsuka, G., Muraki, Y., Noda, K., Papini, P., Perrot, A.-L., Ricciarini, S., Sako, T., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, A., and Turner, W.C.
- Subjects
LHC ,Hadronic interaction models ,Ultra-high energy cosmic ray - Abstract
The inclusive photon energy spectra measured by the Large Hadron Collider forward (LHCf) experiment in the very forward region of LHC proton–proton collisions at s=900GeV are reported. The results from the analysis of 0.30 nb−1 of data collected in May 2010 in the two pseudorapidity regions of η>10.15 and 8.77
- Full Text
- View/download PDF
24. Measurement of zero degree single photon energy spectra for s=7 TeV proton–proton collisions at LHC
- Author
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Adriani, O., Bonechi, L., Bongi, M., Castellini, G., DʼAlessandro, R., Faus, A., Fukatsu, K., Haguenauer, M., Itow, Y., Kasahara, K., Kawade, K., Macina, D., Mase, T., Masuda, K., Matsubara, Y., Menjo, H., Mitsuka, G., Muraki, Y., Nakai, M., Noda, K., Papini, P., Perrot, A.-L., Ricciarini, S., Sako, T., Shimizu, Y., Suzuki, K., Suzuki, T., Taki, K., Tamura, T., Torii, S., Tricomi, A., Turner, W.C., Velasco, J., Viciani, A., and Yoshida, K.
- Subjects
Hadron interaction models ,High Energy Physics::Experiment ,Ultra-high energy cosmic-ray ,LHC - Abstract
In early 2010, the Large Hadron Collider forward (LHCf) experiment measured very forward neutral particle spectra in LHC proton–proton collisions. From a limited data set taken under the best beam conditions (low beam-gas background and low occurrence of pile-up events), the single photon spectra at s=7 TeV and pseudo-rapidity (η) ranges from 8.81 to 8.99 and from 10.94 to infinity were obtained for the first time and are reported in this Letter. The spectra from two independent LHCf detectors are consistent with one another and serve as a cross check of the data. The photon spectra are also compared with the predictions of several hadron interaction models that are used extensively for modeling ultra-high energy cosmic-ray showers. Despite conservative estimates for the systematic errors, none of the models agree perfectly with the measurements. A notable difference is found between the data and the DPMJET 3.04 and PYTHIA 8.145 hadron interaction models above 2 TeV where the models predict higher photon yield than the data. The QGSJET II-03 model predicts overall lower photon yield than the data, especially above 2 TeV in the rapidity range 8.81
- Full Text
- View/download PDF
25. Systematic Construction Method of Multi-Cluster Pauli-Allowed States
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Kato, K., primary, Fukatsu, K., additional, and Tanaka, H., additional
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- 1988
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26. Three-Alpha Potential in 3 and 4 Orthogonality Condition Models
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Fukatsu, K., primary, Kato, K., additional, and Tanaka, H., additional
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- 1989
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27. Expression of centromere protein F (CENP-F) associated with higher FDG uptake on PET/CT, detected by cDNA microarray, predicts high-risk patients with primary breast cancer
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Ishida Jiro, Tamura Katsumi, Yamamoto Junji, Kobayashi Takayuki, Fukatsu Kazuhiko, Asakawa Hideki, Yamamoto Souhei, Tsuda Hitoshi, Kondoh Nobuo, Ueda Shigeto, Abe Yoshiyuki, Yamamoto Mikio, and Mochizuki Hidetaka
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Higher standardized uptake value (SUV) detected by 18F-fluorodeoxyglucose positron emission tomography/computed tomography (FDG PET/CT) correlates with proliferation of primary breast cancer. The purpose of this study is to identify specific molecules upregulated in primary breast cancers with a high SUV and to examine their clinical significance. Methods We compared mRNA expression profiles between 14 tumors with low SUVs and 24 tumors with high SUVs by cDNA microarray. We identified centromere protein F (CENP-F) and CDC6 were upregulated in tumors with high SUVs. RT-PCR and immunohistochemical analyses were performed to validate these data. Clinical implication of CENP-F and CDC6 was examined for 253 archival breast cancers by the tissue microarray. Results The relative ratios of CENP-F and CDC6 expression levels to β-actin were confirmed to be significantly higher in high SUV tumors than in low SUV tumors (p = 0.027 and 0.025, respectively) by RT-PCR. In immunohistochemical analysis of 47 node-negative tumors, the CENP-F expression was significantly higher in the high SUV tumors (74%) than the low SUV tumors (45%) (p = 0.04), but membranous and cytoplasmic CDC6 expressions did not significantly differ between both groups (p = 0.9 each). By the tissue microarray, CENP-F (HR = 2.94) as well as tumor size (HR = 4.49), nodal positivity (HR = 4.1), and Ki67 (HR = 2.05) showed independent impact on the patients' prognosis. Conclusion High CENP-F expression, correlated with high SUV, was the prognostic indicators of primary breast cancer. Tumoral SUV levels may serve as a pretherapeutic indicator of aggressiveness of breast cancer.
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- 2008
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28. Utility of 18F-fluoro-deoxyglucose emission tomography/computed tomography fusion imaging (18F-FDG PET/CT) in combination with ultrasonography for axillary staging in primary breast cancer
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Tamura Katsumi, Hama Yukihiro, Kondo Tadaharu, Kondo Nobuo, Fukatsu Kazuhiko, Omata Jiro, Asakawa Hideki, Tsuda Hitoshi, Ueda Shigeto, Ishida Jiro, Abe Yoshiyuki, and Mochizuki Hidetaka
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Accurate evaluation of axillary lymph node (ALN) involvement is mandatory before treatment of primary breast cancer. The aim of this study is to compare preoperative diagnostic accuracy between positron emission tomography/computed tomography with 18F-fluorodeoxyglucose (18F-FDG PET/CT) and axillary ultrasonography (AUS) for detecting ALN metastasis in patients having operable breast cancer, and to assess the clinical management of axillary 18F-FDG PET/CT for therapeutic indication of sentinel node biopsy (SNB) and preoperative systemic chemotherapy (PSC). Methods One hundred eighty-three patients with primary operable breast cancer were recruited. All patients underwent 18F-FDG PET/CT and AUS followed by SNB and/or ALN dissection (ALND). Using 18F-FDG PET/CT, we studied both a visual assessment of 18F-FDG uptake and standardized uptake value (SUV) for axillary staging. Results In a visual assessment of 18F-FDG PET/CT, the diagnostic accuracy of ALN metastasis was 83% with 58% in sensitivity and 95% in specificity, and when cut-off point of SUV was set at 1.8, sensitivity, specificity, and accuracy were 36, 100, and 79%, respectively. On the other hand, the diagnostic accuracy of AUS was 85% with 54% in sensitivity and 99% in specificity. By the combination of 18F-FDG PET/CT and AUS to the axilla, the sensitivity, specificity, and accuracy were 64, 94, and 85%, respectively. If either 18F-FDG PET uptake or AUS was positive in allixa, the probability of axillary metastasis was high; 50% (6 of 12) in 18F-FDG PET uptake only, 80% (4 of 5) in AUS positive only, and 100% (28 of 28) in dual positive. By the combination of AUS and 18F-FDG PET/CT, candidates of SNB were more appropriately selected. The axillary 18F-FDG uptake was correlated with the maximum size and nuclear grade of metastatic foci (p = 0.006 and p = 0.03). Conclusion The diagnostic accuracy of 18F-FDG PET/CT was shown to be nearly equal to ultrasound, and considering their limited sensitivities, the high radiation exposure by 18F-FDG PET/CT and also costs of the examination, it is likely that AUS will be more cost-effective in detecting massive axillary tumor burden. However, when we cannot judge the axillary staging using AUS alone, metabolic approach of 18F-FDG PET/CT for axillary staging would enable us a much more confident diagnosis.
- Published
- 2008
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29. Utility of 18F-fluoro-deoxyglucose emission tomography/computed tomography fusion imaging (18F-FDG PET/CT) in combination with ultrasonography for axillary staging in primary breast cancer.
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Ueda S, Tsuda H, Asakawa H, Omata J, Fukatsu K, Kondo N, Kondo T, Hama Y, Tamura K, Ishida J, Abe Y, Mochizuki H, Ueda, Shigeto, Tsuda, Hitoshi, Asakawa, Hideki, Omata, Jiro, Fukatsu, Kazuhiko, Kondo, Nobuo, Kondo, Tadaharu, and Hama, Yukihiro
- Abstract
Background: Accurate evaluation of axillary lymph node (ALN) involvement is mandatory before treatment of primary breast cancer. The aim of this study is to compare preoperative diagnostic accuracy between positron emission tomography/computed tomography with 18F-fluorodeoxyglucose (18F-FDG PET/CT) and axillary ultrasonography (AUS) for detecting ALN metastasis in patients having operable breast cancer, and to assess the clinical management of axillary 18F-FDG PET/CT for therapeutic indication of sentinel node biopsy (SNB) and preoperative systemic chemotherapy (PSC).Methods: One hundred eighty-three patients with primary operable breast cancer were recruited. All patients underwent 18F-FDG PET/CT and AUS followed by SNB and/or ALN dissection (ALND). Using 18F-FDG PET/CT, we studied both a visual assessment of 18F-FDG uptake and standardized uptake value (SUV) for axillary staging.Results: In a visual assessment of 18F-FDG PET/CT, the diagnostic accuracy of ALN metastasis was 83% with 58% in sensitivity and 95% in specificity, and when cut-off point of SUV was set at 1.8, sensitivity, specificity, and accuracy were 36, 100, and 79%, respectively. On the other hand, the diagnostic accuracy of AUS was 85% with 54% in sensitivity and 99% in specificity. By the combination of 18F-FDG PET/CT and AUS to the axilla, the sensitivity, specificity, and accuracy were 64, 94, and 85%, respectively. If either 18F-FDG PET uptake or AUS was positive in allixa, the probability of axillary metastasis was high; 50% (6 of 12) in 18F-FDG PET uptake only, 80% (4 of 5) in AUS positive only, and 100% (28 of 28) in dual positive. By the combination of AUS and 18F-FDG PET/CT, candidates of SNB were more appropriately selected. The axillary 18F-FDG uptake was correlated with the maximum size and nuclear grade of metastatic foci (p = 0.006 and p = 0.03).Conclusion: The diagnostic accuracy of 18F-FDG PET/CT was shown to be nearly equal to ultrasound, and considering their limited sensitivities, the high radiation exposure by 18F-FDG PET/CT and also costs of the examination, it is likely that AUS will be more cost-effective in detecting massive axillary tumor burden. However, when we cannot judge the axillary staging using AUS alone, metabolic approach of 18F-FDG PET/CT for axillary staging would enable us a much more confident diagnosis. [ABSTRACT FROM AUTHOR]- Published
- 2008
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30. Phosphorylation of plasma membrane H + -ATPase Thr881 participates in light-induced stomatal opening.
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Hayashi Y, Fukatsu K, Takahashi K, Kinoshita SN, Kato K, Sakakibara T, Kuwata K, and Kinoshita T
- Subjects
- Phosphorylation, Light, Plant Stomata metabolism, Proton-Translocating ATPases genetics, Proton-Translocating ATPases metabolism, Cell Membrane metabolism, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism
- Abstract
Plasma membrane (PM) H
+ -ATPase is crucial for light-induced stomatal opening and phosphorylation of a penultimate residue, Thr948 (pen-Thr, numbering according to Arabidopsis AHA1) is required for enzyme activation. In this study, a comprehensive phosphoproteomic analysis using guard cell protoplasts from Vicia faba shows that both red and blue light increase the phosphorylation of Thr881, of PM H+ -ATPase. Light-induced stomatal opening and the blue light-induced increase in stomatal conductance are reduced in transgenic Arabidopsis plants expressing mutant AHA1-T881A in aha1-9, whereas the blue light-induced phosphorylation of pen-Thr is unaffected. Auxin and photosynthetically active radiation induce the phosphorylation of both Thr881 and pen-Thr in etiolated seedlings and leaves, respectively. The dephosphorylation of phosphorylated Thr881 and pen-Thr are mediated by type 2 C protein phosphatase clade D isoforms. Taken together, Thr881 phosphorylation, in addition of the pen-Thr phosphorylation, are important for PM H+ -ATPase function during physiological responses, such as light-induced stomatal opening in Arabidopsis thaliana., (© 2024. The Author(s).)- Published
- 2024
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31. In vitro feeding of all life stages of two-host Hyalomma excavatum and Hyalomma scupense and three-host Hyalomma dromedarii ticks.
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Elati K, Benyedem H, Fukatsu K, Hoffmann-Köhler P, Mhadhbi M, Bakırcı S, Bilgiç HB, Karagenç T, Darghouth MA, and Nijhof AM
- Subjects
- Animals, Female, Life Cycle Stages, Nymph, Larva, Ticks parasitology, Ixodidae
- Abstract
Ticks are blood-sucking ectoparasites and can transmit various pathogens of medical and veterinary relevance. The life cycle of ticks can be completed under laboratory conditions on experimental animals, but the artificial feeding of ticks has attracted increased interest as an alternative method. This study represents the first report on the successful in vitro feeding of all life stages of two-host tick species, Hyalomma scupense and Hyalomma excavatum, and the three-host tick Hyalomma dromedarii. The attachment and engorgement rates of adults were 84% (21/25) and 76% (19/25) for H. scupense females. For adult H. excavatum and H. dromedarii, 70% (21/30) and 34.4% (11/32) of the females attached and all attached females successfully fed to repletion. The oviposition rates of the artificially fed females were 36.4%, 57.1% and 63.1% for H. dromedarii, H. excavatum and H. scupense, respectively, with a reproductive efficiency index varying between 44.3 and 60.7%. For the larvae, the attachment and engorgement rates were 44.2% (313/708) and 42.8% (303/708) for H. dromedarii, 70.5% (129/183) and 56.8% (104/183) for H. excavatum and 92.6% (113/122) and 55.7% (68/122) for H. scupense. The attachment and engorgement rates for the nymphs were 90.2% (129/143) and 47.6% (68/143) for H. dromedarii, 66.7% (34/51) and 41.2% (21/51) for H. excavatum, and 44.1% (30/68) and 36.8% (25/68) for H. scupense. Molting rates of the immature stages varied between 71.3% (216/303) and 100% (68/68) for the larvae and between 61.9% (13/21) and 96% (24/25) for the nymphs. The successful in vitro feeding of all stages of the three Hyalomma species makes this method a valuable tool for tick research, with potential applications in studies on the pathogens transmitted by these tick species such as Theileria annulata., (© 2024. The Author(s).)
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- 2024
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32. Reduced volume and altered composition of paraspinal muscles in Marfan syndrome: A retrospective cohort study.
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Yoshida Y, Doi T, Oka H, Kato S, Ohtomo N, Nakamoto H, Takeda N, Inuzuka R, Yagi H, Oshima Y, Tanaka S, Fukatsu K, and Taniguchi Y
- Subjects
- Humans, Retrospective Studies, Magnetic Resonance Imaging methods, Paraspinal Muscles pathology, Lumbar Vertebrae surgery, Scoliosis etiology, Scoliosis surgery, Scoliosis pathology, Marfan Syndrome complications
- Abstract
Retrospective cohort study. Spinal deformities in patients with Marfan syndrome (MFS) are distinct from those in patients with idiopathic scoliosis (IS). It is more prone to progression and more likely to present with sagittal malalignment than IS. However, the etiology of this characteristic spinal deformity in MFS remains unclear. This study aimed to determine the spinal musculature characteristics in patients with MFS on the hypothesis that the paraspinal muscles of patients with MFS would be qualitatively or quantitatively different from those of patients with IS. Seventeen consecutive patients with MFS aged 25 years or younger undergoing surgery for scoliosis in our hospital were compared with age- and sex-matched patients with IS undergoing surgery for scoliosis. The body size-adjusted relative cross-sectional area (rCSA), fatty infiltration ratio (FI%), and relative functional cross-sectional area (rFCSA) of the psoas muscles (PM) and paravertebral muscles (PVM) at L3/4 and L4/5 were measured using preoperative T2-weighted magnetic resonance imaging. Functional CSA was defined as total CSA minus the fatty infiltration area of each muscle and rFCSA was calculated as the body size-adjusted functional CSA. The rCSA of the PM at L3/4 and L4/5 was significantly smaller in the MFS group than in the IS group (L3/4, P = .021; L4/5, P = .002). The FI% of the PM at L4/5 was significantly higher in the MFS group (P = .044). Consequently, the rFCSA of the PM at L3/4 and L4/5 and the rFCSA of the PVM at L3/4 in the MFS group were significantly smaller than those in the IS group (PM at L3/4, P = .021; PM at L4/5, P = .001; PVM at L3/4, P = .025). Compared with patients with IS, patients with MFS exhibited significantly decreased body-size-adjusted CSA of the PM and reduced body-size-adjusted functional CSA of the PVM and PM. These findings may partially explain the characteristics of distinctive spinal deformities in patients with MFS., Competing Interests: The authors have no funding and conflicts of interest to disclose., (Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2023
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33. Correlations between class I glucose transporter expression patterns and clinical outcomes in non-small cell lung cancer.
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Nakanishi Y, Iwai M, Hirotani Y, Kato R, Tanino T, Nishimaki-Watanabe H, Nozaki F, Ohni S, Tang X, Masuda S, and Sasaki-Fukatsu K
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- Humans, Glucose Transport Proteins, Facilitative genetics, Glucose Transport Proteins, Facilitative metabolism, Glucose Transporter Type 3 genetics, Interleukin-8 genetics, Interleukin-8 metabolism, Carcinoma, Non-Small-Cell Lung genetics, Lung Neoplasms genetics, Adenocarcinoma, Carcinoma, Squamous Cell
- Abstract
Background: Glucose transporters (GLUTs) are highly expressed in various cancers. However, the implications of these variable expression patterns are unclear. This study aimed to clarify the correlation between class I GLUT expression patterns and clinical outcomes in non-small cell lung cancer (NSCLC), including their potential role in inflammatory signaling., Methods: Biopsy tissues from 132 patients with NSCLC (92 adenocarcinomas [ADC] and 40 squamous cell carcinomas [SQCC]) were analyzed. mRNA expression levels of class I GLUTs (solute carrier 2A [SLC2A]1, SLC2A2, SLC2A3, and SLC2A4) and inflammation-related molecules (toll-like receptors TLR4, RelA/p65, and interleukins IL8 and IL6) were measured. Cellular localization of GLUT3 and GLUT4 was investigated using immunofluorescence., Results: Single, combined, and negative GLUT (SLC2A) expression were observed in 27/92 (29.3%), 27/92 (29.3%), and 38/92 (41.3%, p < 0.001) of ADC and 8/40 (20.0%), 29/40 (72.5%, p < 0.001), and 3/40 (7.5%) of SQCC, respectively. In ADC, the single SLC2A3-expressed group had a significantly poorer prognosis, whereas the single SLC2A4-expressed group had a significantly better prognosis. The combined expression groups showed no significant difference. SLC2A expression was not correlated with SQCC prognosis. SLC2A4 expression correlated with lower IL8 expression. GLUT3 and GLUT4 expressions were localized in the tumor cytoplasm., Conclusions: In lung ADC, single SLC2A3 expression correlated with poor prognosis, whereas single SLC2A4 expression correlated with better prognosis and lower IL8 expression. GLUT3 expression, which is increased by IL8 overexpression, may be suppressed by increasing the expression of GLUT4 through decreased IL8 expression., (© 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.)
- Published
- 2023
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34. PECT1, a rate-limiting enzyme in phosphatidylethanolamine biosynthesis, is involved in the regulation of stomatal movement in Arabidopsis.
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Negi J, Obata T, Nishimura S, Song B, Yamagaki S, Ono Y, Okabe M, Hoshino N, Fukatsu K, Tabata R, Yamaguchi K, Shigenobu S, Yamada M, Hasebe M, Sawa S, Kinoshita T, Nishida I, and Iba K
- Subjects
- Carbon Dioxide metabolism, Phosphatidylethanolamines metabolism, Plant Stomata metabolism, Adenosine Triphosphatases metabolism, Light, Proton-Translocating ATPases metabolism, Arabidopsis metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism
- Abstract
An Arabidopsis mutant displaying impaired stomatal responses to CO
2 , cdi4, was isolated by a leaf thermal imaging screening. The mutated gene PECT1 encodes CTP:phosphorylethanolamine cytidylyltransferase. The cdi4 exhibited a decrease in phosphatidylethanolamine levels and a defect in light-induced stomatal opening as well as low-CO2 -induced stomatal opening. We created RNAi lines in which PECT1 was specifically repressed in guard cells. These lines are impaired in their stomatal responses to low-CO2 concentrations or light. Fungal toxin fusicoccin (FC) promotes stomatal opening by activating plasma membrane H+ -ATPases in guard cells via phosphorylation. Arabidopsis H+ -ATPase1 (AHA1) has been reported to be highly expressed in guard cells, and its activation by FC induces stomatal opening. The cdi4 and PECT1 RNAi lines displayed a reduced stomatal opening response to FC. However, similar to in the wild-type, cdi4 maintained normal levels of phosphorylation and activation of the stomatal H+ -ATPases after FC treatment. Furthermore, the cdi4 displayed normal localization of GFP-AHA1 fusion protein and normal levels of AHA1 transcripts. Based on these results, we discuss how PECT1 could regulate CO2 - and light-induced stomatal movements in guard cells in a manner that is independent and downstream of the activation of H+ -ATPases. [Correction added on 15 May 2023, after first online publication: The third sentence is revised in this version.]., (© 2023 Society for Experimental Biology and John Wiley & Sons Ltd.)- Published
- 2023
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35. Multicenter randomized control study of the efficacy of SO clip in colorectal endoscopic submucosal dissection (ESD). (SO clip study in colorectal ESD): Randomized controlled trial.
- Author
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Taki S, Iguchi M, Fukatsu K, Shimokawa T, Kinoshita I, Syunsuke O, Maekita T, Kinoshita J, Takao M, and Kitano M
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- Humans, Surgical Instruments, Traction, Treatment Outcome, Endoscopic Mucosal Resection methods, Colorectal Neoplasms surgery, Carcinoma
- Abstract
Introduction: Endoscopic submucosal dissection (ESD) allows the en bloc resection of colorectal epithelial tumors regardless of size. Although ESD is minimally invasive and yields favorable outcomes, it is technically difficult and requires a long procedure time. In addition, colorectal ESD is associated with a particularly high risk of complications, due to the thin bowel wall, bowel flexion, and peristalsis.Direct visualization of the submucosal layer by traction of the lesion after mucosal dissection would make ESD performance easier. S-O clips traction lesions toward the lumen, facilitating direct visualization of the submucosal layer, resulting in efficient dissection due to the traction effect and adequate dissection depth. Use of this traction device can contribute to shortening the procedure time and reducing the risk of complications. This multicenter randomized controlled trial will evaluate the usefulness of the S-O clip in colorectal ESD and assess the procedure time and frequency of complications associated with the procedure., Methods/design: This multicenter, randomized control trial will enroll 200 patients at 4 hospitals in Japan undergoing ESD for colorectal epithelial tumors. Patients who meet the inclusion and exclusion criteria will be randomized to undergo ESD using S-O clips or conventional ESD. Patients will be randomized by a computer-generated random sequence with stratification by operator experience (trainee or expert), tumor location (colon/rectum), and institution. The primary endpoint will be ESD procedure time, defined as the time from the start of the local injection into the submucosal layer to the end of dissection. Other outcomes will include the rates of procedural complications, en bloc resection and cure., Discussion: ESD using the S-O clip is expected to shorten procedure time, reduce the incidence of adverse events, and standardize the procedure. This study may resolve clinical questions about whether ESD using the S-O clip traction device is more effective and safer than conventional ESD., Competing Interests: The authors have no conflicts of interest to disclose., (Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2023
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36. ETS1 loss in mice impairs cardiac outflow tract septation via a cell migration defect autonomous to the neural crest.
- Author
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Lin L, Pinto A, Wang L, Fukatsu K, Yin Y, Bamforth SD, Bronner ME, Evans SM, Nie S, Anderson RH, Terskikh AV, and Grossfeld PD
- Subjects
- Animals, Humans, Mice, Cell Movement genetics, Heart, Organogenesis, Heart Defects, Congenital, Neural Crest, Proto-Oncogene Protein c-ets-1 genetics
- Abstract
Ets1 deletion in some mouse strains causes septal defects and has been implicated in human congenital heart defects in Jacobsen syndrome, in which one copy of the Ets1 gene is missing. Here, we demonstrate that loss of Ets1 in mice results in a decrease in neural crest (NC) cells migrating into the proximal outflow tract cushions during early heart development, with subsequent malalignment of the cushions relative to the muscular ventricular septum, resembling double outlet right ventricle (DORV) defects in humans. Consistent with this, we find that cultured cardiac NC cells from Ets1 mutant mice or derived from iPS cells from Jacobsen patients exhibit decreased migration speed and impaired cell-to-cell interactions. Together, our studies demonstrate a critical role for ETS1 for cell migration in cardiac NC cells that are required for proper formation of the proximal outflow tracts. These data provide further insights into the molecular and cellular basis for development of the outflow tracts, and how perturbation of NC cells can lead to DORV., (© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Published
- 2022
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37. Elevated CO 2 induces rapid dephosphorylation of plasma membrane H + -ATPase in guard cells.
- Author
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Ando E, Kollist H, Fukatsu K, Kinoshita T, and Terashima I
- Subjects
- Plant Stomata physiology, Carbon Dioxide pharmacology, Carbon Dioxide metabolism, Proton-Translocating ATPases metabolism, Cell Membrane metabolism, Light, Arabidopsis metabolism, Arabidopsis Proteins metabolism
- Abstract
Light induces stomatal opening, which is driven by plasma membrane (PM) H
+ -ATPase in guard cells. The activation of guard-cell PM H+ -ATPase is mediated by phosphorylation of the penultimate C-terminal residue, threonine. The phosphorylation is induced by photosynthesis as well as blue light photoreceptor phototropin. Here, we investigated the effects of cessation of photosynthesis on the phosphorylation level of guard-cell PM H+ -ATPase in Arabidopsis thaliana. Immunodetection of guard-cell PM H+ -ATPase, time-resolved leaf gas-exchange analyses and stomatal aperture measurements were carried out. We found that light-dark transition of leaves induced dephosphorylation of the penultimate residue at 1 min post-transition. Gas-exchange analyses confirmed that the dephosphorylation is accompanied by an increase in the intercellular CO2 concentration, caused by the cessation of photosynthetic CO2 fixation. We discovered that CO2 induces guard-cell PM H+ -ATPase dephosphorylation as well as stomatal closure. Interestingly, reverse-genetic analyses using guard-cell CO2 signal transduction mutants suggested that the dephosphorylation is mediated by a mechanism distinct from the established CO2 signalling pathway. Moreover, type 2C protein phosphatases D6 and D9 were required for the dephosphorylation and promoted stomatal closure upon the light-dark transition. Our results indicate that CO2 -mediated dephosphorylation of guard-cell PM H+ -ATPase underlies stomatal closure., (© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)- Published
- 2022
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38. Diffusion MR Imaging with T2-based Water Suppression (T2wsup-dMRI).
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Kimura T, Yamashita K, and Fukatsu K
- Subjects
- Artifacts, Brain diagnostic imaging, Brain pathology, Diffusion Magnetic Resonance Imaging, Magnetic Resonance Imaging methods, Image Processing, Computer-Assisted methods, Water
- Abstract
Purpose: This study proposes and assesses a new diffusion MRI (dMRI) technique to solve problems related to the quantification of parameter maps (apparent diffusion coefficient [ADC] or mean diffusivity [MD], fractional anisotropy [FA]) and misdrawing of fiber tractography (FT) due to cerebrospinal fluid (CSF)-partial volume effects (PVEs) for brain tissues by combining with the T2-based water suppression (T2wsup) technique., Methods: T2wsup-diffusion-weighted imaging (DWI) images were obtained by subtracting those images from the acquired multi-b value (b) DWI images after correcting the signal intensities of multiecho time (TE) images using long TE water signal-dominant images. Quantitative parameter maps and FT were obtained from minimum data points and were compared with those using the standard (without wsup) DWI method, and partly compared with those obtained using other alternative DWI methods of applying fluid attenuation inversion recovery (FLAIR), non-b-zero (NBZ) by theoretical or noise-added simulation and MR images., Results: In the T2wsup-dMRI method, the hyperintense artifacts due to CSF-PVEs in MRI data were dramatically suppressed even at lower b (≲ 500 s/mm
2 ) while keeping the tissue SNR. The quantitative parameter map values became precisely close to the pure tissue values precisely even in water (CSF) PVE voxels in healthy brain tissues (T2 ≲ 100 ms). Furthermore, the fiber tracts were correctly connected, particularly at the fornix in closest contact to the CSF., Conclusion: Solving the problem of CSF-PVE in the current dMRI technique using our proposed T2wsup-dMRI technique is easy, with higher SNR than those obtained with FLAIR or NBZ methods when applying to healthy brain tissues. The proposed T2wsup-dMRI could be useful in clinical settings, although further optimization of the pulse sequence and processing techniques and clinical assessments are required, particularly for long T2 lesions.- Published
- 2022
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39. Quantitative analysis of organophosphate pesticides and dialkylphosphates in duplicate diet samples to identify potential sources of measured urinary dialkylphosphates in Japanese women.
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Tsuchiyama T, Ito Y, Oya N, Nomasa K, Sato H, Minato K, Kitamori K, Oshima S, Minematsu A, Niwa K, Katsuhara M, Fukatsu K, Miyazaki H, Ebara T, and Kamijima M
- Subjects
- Child, Diet, Environmental Exposure analysis, Female, Humans, Japan, Organophosphates, Organophosphorus Compounds, Insecticides analysis, Pesticides
- Abstract
Increased levels of dialkylphosphates (DAP) in maternal urine are associated with a variety of adverse developmental outcomes in children. Although urinary DAP levels are usually considered to be a marker of exposure to organophosphate (OP) pesticides, excretion of DAP may also increase by ingesting preformed DAP. To date, no study has quantitatively assessed the possible contribution of the dietary intake of preformed DAP and OP pesticides to urinary levels of DAP. Therefore, we aimed to estimate the levels of 6 DAPs and 84 OP pesticides in duplicate diet samples and urine samples collected from 73 women living in urban areas of Japan in 2018. DAP and OP pesticides were detected in 94% and 45% of diet samples, while DAP was detected in 100% of urinary samples, respectively. The average daily intake of preformed DAP was significantly higher than that of parent OP pesticides in our participants. Dimethylphosphate and diethylphosphate were predominant in the preformed DAP, and the estimated average daily intake of total amount of DAP was 78.3 nmol. Fruits and vegetables were the major dietary sources of DAP. Dietary intake of DAP was positively associated with urinary DAP levels, suggesting that a considerable amount of urinary DAP was derived from ingesting preformed DAP. Our results show that attributing urinary DAP levels exclusively to OP pesticide exposure would result in a substantial overestimation of the exposure level. Therefore, the urinary levels of DAP may not be suitable for evaluating OP pesticide exposure in the general urban population., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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40. Traction method versus conventional endoscopic submucosal dissection for gastric epithelial neoplasms: A randomized controlled trial.
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Kinoshita J, Iguchi M, Maekita T, Wan K, Shimokawa T, Fukatsu K, Ito D, Taki S, Nishimoto M, Takao M, Tabata Y, Mukai Y, and Kitano M
- Subjects
- Humans, Prospective Studies, Traction, Treatment Outcome, Endoscopic Mucosal Resection methods, Neoplasms, Glandular and Epithelial, Stomach Neoplasms etiology, Stomach Neoplasms surgery
- Abstract
Introduction: Endoscopic submucosal dissection (ESD) is an advanced therapeutic technique for en bloc resection of superficial gastrointestinal neoplasms. Although gastric ESD is minimally invasive and provides favorable outcomes, it is technically difficult and requires a long procedure time for dissection. The traction-assisted approach overcomes some of the difficulties of gastric ESD, but its ability to reduce the procedure time remains unclear. The traction-assisted approach using dental floss and a clip did not reduce procedure time in the total population, but it reduced procedure time for lesions limited to the greater curvature of the upper or middle of the stomach. Although the traction direction of the clip-with-line method may be limited to the oral side via the cardia, EndoTrac ESD may provide flexible traction at any time during the procedure. This prospective randomized control study has been designed to compare the efficacy and safety of EndoTrac and conventional gastric ESD., Methods/design: This multicenter, randomized control trial will enroll 150 patients at 2 hospitals in Japan undergoing EndoTrac or conventional ESD for gastric epithelial neoplasia. Patients with a single gastric epithelial neoplasm who meet the inclusion and exclusion criteria will be randomized to EndoTrac or conventional ESD. Patients will be randomized by a computer-generated random sequence with stratification by operator experience, tumor size, tumor location, and institution. The primary endpoint will be ESD procedure time, defined as the time from the start of the submucosal injection to the completion of resection. Other outcomes will include the rates of adverse events and pathological curability., Discussion: The ability of EndoTrac ESD to reduce the long procedure time and/or adverse events observed with conventional ESD can not only reduce physical stress on the patient, but can also reduce length of hospital stay and medical costs. Reduced technical difficulty will contribute to the widespread adoption of this ESD technique worldwide., Trial Registration: University Hospital Medial Information Network Clinical Trials Registry (UMIN-CTR), ID: 000044450; Registered on June 6, 2021.https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000050485., Protocol Version Number: 1.1, March 1, 2022. Patient enrolment began on June 6, 2021 and is expected to be completed by July 19, 2025., Competing Interests: The authors have no conflicts of interest to disclose., (Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2022
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41. Identification of Abscisic Acid-Dependent Phosphorylated Basic Helix-Loop-Helix Transcription Factors in Guard Cells of Vicia faba by Mass Spectrometry.
- Author
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Hayashi Y, Takahashi Y, Fukatsu K, Tada Y, Takahashi K, Kuwata K, Suzuki T, and Kinoshita T
- Abstract
An unknown 61 kDa protein is phosphorylated by abscisic acid (ABA)-activated protein kinase in response to ABA and binds to 14-3-3 protein in a phosphorylation-dependent manner in guard-cell protoplasts (GCPs) from Vicia faba . Subsequently, ABA-dependent phosphorylated proteins were identified as basic helix-loop-helix transcription factors, named ABA-responsive kinase substrates (AKSs) in GCPs from Arabidopsis thaliana . However, whether the 61 kDa protein in Vicia GCPs is an AKS is unclear. We performed immunoprecipitation of ABA-treated Vicia GCPs using anti-14-3-3 protein antibodies and identified several AKS isoforms in V. faba (VfAKSs) by mass spectrometry. The 61 kDa protein was identified as VfAKS1. Phosphoproteomic analysis revealed that VfAKSs are phosphorylated at Ser residues, which are important for 14-3-3 protein binding and monomerisation, in response to ABA in GCPs. Orthologs of AtABCG40, an ABA importer in guard cells, and CHC1, a clathrin heavy chain and a regulator of stomatal movement, also co-immunoprecipitated with 14-3-3 protein from guard cells., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Hayashi, Takahashi, Fukatsu, Tada, Takahashi, Kuwata, Suzuki and Kinoshita.)
- Published
- 2021
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42. Synthetic MRI with T 2 -based Water Suppression to Reduce Hyperintense Artifacts due to CSF-Partial Volume Effects in the Brain.
- Author
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Kimura T, Yamashita K, and Fukatsu K
- Subjects
- Brain diagnostic imaging, Contrast Media, Humans, Magnetic Resonance Imaging, Artifacts, Water
- Abstract
Purpose: Our purpose was to assess our proposed new synthetic MRI (synMRI) technique, combined with T
2 -based water suppression (T2 wsup), to reduce cerebral spinal fluid (CSF)-partial volume effects (PVEs). These PVEs are problematic in the T2 -weighted fluid-attenuation inversion recovery (FLAIR) images obtained by conventional synMRI techniques., Methods: Our T2 wsup was achieved by subtracting additionally acquired long TE spin echo (SE) images of water signals dominant from the originally acquired images after T2 decay correction and a masking on the long TE image using the water volume (Vw ) map to preserve tissue SNR, followed by quantitative mapping and then calculation of the synthetic images. A simulation study based on a two-compartment model including tissue and water in a voxel and a volunteer MR study were performed to assess our proposed method. Parameters of long TE and a threshold value in the masking were assessed and optimized experimentally. Quantitative parameter maps of standard and with T2 wsup were generated, then wsup-synthetic FLAIR and SE images were calculated using those suitable combinations and compared., Results: Our simulation clarified that the CSF-PVE artifacts in the standard synthetic FLAIR increase T2 as the water volume increases in a voxel, and the volunteer MR brain study demonstrated that the hyperintense artifacts on synthetic images were reduced to <10% of Vw in those with the standard synMRI while keeping the tissue SNR by selecting optimal masking parameters on additional long TE images of TE = 300 ms. In addition, the wsup-synthetic SE provided better gray-white matter contrasts compared with the wsup-synthetic FLAIR while keeping CSF suppression., Conclusion: Our proposed T2 wsup-synMRI technique makes it easy to reduce the CSF-PVE artifacts problematic in the synthetic FLAIR images using the current synMRI technique by adding long TE images and simple processing. Although further optimizations in data acquisition and processing techniques are required before actual clinical use, we expect our technique to become clinically useful.- Published
- 2021
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43. Identification of Genes Preferentially Expressed in Stomatal Guard Cells of Arabidopsis thaliana and Involvement of the Aluminum-Activated Malate Transporter 6 Vacuolar Malate Channel in Stomatal Opening.
- Author
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Ye W, Koya S, Hayashi Y, Jiang H, Oishi T, Kato K, Fukatsu K, and Kinoshita T
- Abstract
Stomatal guard cells (GCs) are highly specialized cells that respond to various stimuli, such as blue light (BL) and abscisic acid, for the regulation of stomatal aperture. Many signaling components that are involved in the stomatal movement are preferentially expressed in GCs. In this study, we identified four new such genes in addition to an aluminum-activated malate transporter, ALMT6 , and GDSL lipase, Occlusion of Stomatal Pore 1 ( OSP1 ), based on the expression analysis using public resources, reverse transcription PCR, and promoter-driven β-glucuronidase assays. Some null mutants of GC-specific genes evidenced altered stomatal movement. We further investigated the role played by ALMT6, a vacuolar malate channel, in stomatal opening. Epidermal strips from an ALMT6- null mutant exhibited defective stomatal opening induced by BL and fusicoccin, a strong plasma membrane H
+ -ATPase activator. The deficiency was enhanced when the assay buffer [Cl- ] was low, suggesting that malate and/or Cl- facilitate efficient opening. The results indicate that the GC-specific genes are frequently involved in stomatal movement. Further detailed analyses of the hitherto uncharacterized GC-specific genes will provide new insights into stomatal regulation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Ye, Koya, Hayashi, Jiang, Oishi, Kato, Fukatsu and Kinoshita.)- Published
- 2021
- Full Text
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44. Benzpyrimoxan: Design, synthesis, and biological activity of a novel insecticide.
- Author
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Satoh E, Kasahara R, Fukatsu K, Aoki T, Harayama H, and Murata T
- Abstract
Benzpyrimoxan (5-(1,3-dioxan-2-yl)-4-{[4-(trifluoromethyl)phenyl]methoxy}pyrimidine, NNI-1501) was discovered as a novel insecticide structurally characterized by a pyrimidine derivative substituted with 1,3-dioxanyl and 4-trifluoromethylbenzyloxy groups. The compound showed remarkable activity against nymphs of rice planthoppers, including strains resistant to existing insecticides. Furthermore, benzpyrimoxan had low adverse effects on pollinators and beneficial arthropods. Because of these features, benzpyrimoxan is expected to be a suitable part of an integrated pest management strategy. In this report, the history of the discovery to reach benzpyrimoxan and details of the structure-activity relationships are described., (© Pesticide Science Society of Japan 2021. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License (https://creativecommons.org/licenses/by-nc-nd/4.0/).)
- Published
- 2021
- Full Text
- View/download PDF
45. Evaluation of the accuracy of urine analyzers in dogs and cats.
- Author
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Mie K, Hayashi A, Nishida H, Okamoto M, Yasuda K, Nakata M, Fukatsu K, Matsunami N, Yamashita S, Ohashi F, and Akiyoshi H
- Subjects
- Animals, Urinalysis instrumentation, Bilirubin urine, Cats urine, Dogs urine, Ketones urine, Proteinuria veterinary, Urinalysis veterinary
- Abstract
The accuracy of urine analyzers used for dogs and cats has remained uncertain. This study examines the agreement between results of urine analysis obtained using two devices marketed for animals and for humans and the results of quantitative biochemical analysis. The degrees of concordance for bilirubin and ketones in the same category were ~80%, but for pH these were only ~60% in dogs and cats. Degrees of concordance for protein and the UP/C ratio clearly differed between the devices for animals and humans. We found that values for bilirubin and ketones obtained using urine analyzers may be reliable, but pH is unlikely to be accurate enough to be clinically useful for dogs and cats.
- Published
- 2019
- Full Text
- View/download PDF
46. Migration of a Percutaneous Endoscopic Gastrojejunostomy Tube into the Colon with Small Intestinal Telescoping.
- Author
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Taki S, Maekita T, Sakata M, Fukatsu K, Maeda Y, Iguchi M, Ito H, and Kitano M
- Abstract
Continuous duodenal levodopa/carbidopa intestinal gel delivery by a gastrostomy infusion system improves control of Parkinson's disease. The overall complication rates of percutaneous endoscopic gastrojejunostomy were reported to be 41% and 59% for immediate and delayed adverse events, respectively. A 72-year-old woman underwent percutaneous endoscopic gastrojejunostomy using the delivery system noted above. Abdominal pain and vomiting occurred 3 months later. Esophagogastroduodenoscopy showed a longitudinal ulcer extending from the lower gastric body to the ileum end, with small intestinal telescoping. Colonoscopy showed a large bezoar of food residue that was attached around the tip of the tube, reaching the ascending colon, which may have acted as an anchor. Thus, the gastric antrum and small intestine were shortened with telescoping. This complication was resolved by crushing the bezoar with forceps during colonoscopy and can be prevented by consuming a fiber-free diet and periodic exchanges of the tube using esophagogastroduodenoscopy.
- Published
- 2019
- Full Text
- View/download PDF
47. Late-Evening Carbohydrate and Branched-Chain Amino Acid Snacks Improve the Nutritional Status of Patients Undergoing Hepatectomy Based on Bioelectrical Impedance Analysis of Body Composition.
- Author
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Kobayashi K, Kaneko J, Yamaguchi T, Kawaguchi Y, Arita J, Akamatsu N, Ishizawa T, Sekine R, Ijichi H, Kubota N, Fukatsu K, Kokudo N, and Hasegawa K
- Abstract
Background: This prospective study measured body composition based on bioelectrical impedance analysis (BIA) in relation to preoperative and postoperative nutritional support and status in patients undergoing liver surgery., Methods: Thirty-seven patients with impaired liver function (indocyanine green retention rate at 15 min >15%) undergoing hepatectomy for hepatocellular carcinoma or colorectal liver metastasis were enrolled. The control group ( n = 10) received no nutritional supplementation. The late-evening snack (LES, n = 26) group received a 210-kcal snack comprising a carbohydrate with branched-chain amino acids for 2 weeks before surgery through to 12 weeks after surgery. BIA of body composition, including body cell mass and skeletal muscle volume, was performed., Results: Although there was no sarcopenia based on the consensus report of the Asian Working Group 2 weeks before surgery, the skeletal muscle volumes in the control and LES groups were at the lower limit of the normal range. Body cell mass and skeletal muscle volume were significantly lower in the control group than in the LES group at 4 ( p = 0.03) and 12 ( p = 0.02) weeks after surgery., Conclusion: Late-evening carbohydrate and branched-chain amino acid snack supplementation may improve nutritional status in patients with impaired liver function undergoing hepatectomy., Competing Interests: The authors declare no conflicts of interest., (Copyright © 2019 by S. Karger AG, Basel.)
- Published
- 2019
- Full Text
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48. Role of nutrition in gastroenterological surgery.
- Author
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Fukatsu K
- Abstract
Nutrition plays important roles in recovery after gastroenterological surgery. Severe surgical stress increases muscle breakdown and lipolysis, thereby accelerating wound healing and enhancing host defense against microbes. Malnourished patients have insufficient amounts of muscle and body fat. Therefore, they may not appropriately respond to surgical stress. Perioperative nutritional therapy maintaining nutritional status reduces postoperative complications and accelerates recovery after surgery, particularly for malnourished patients. In addition, perioperative oral or enteral nutrition is now recommended for preserving host defense mechanisms against microbes. Lack of enteral nutrition impairs gut and hepatic immunity, systemic mucosal defense and peritoneal host defense, even when nutrient amounts supplied by parenteral nutrition are adequate. Thus, surgeons should avoid no oral or enteral nutrition periods. Supplemental administration of specific nutrients such as glutamine, arginine and ω-3 fatty acids is termed "immunonutrition", and is expected to reduce the morbidity of infectious complications and length of hospital stay. Nutritional therapy is important even after discharge to maintain body weight and compensate for abnormalities in the digestion and absorption of nutrients. Understanding the significance of nutrition in gastroenterological patients leads to better outcomes.
- Published
- 2019
- Full Text
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49. Identification and Characterization of Compounds that Affect Stomatal Movements.
- Author
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Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, and Kinoshita T
- Subjects
- Abscisic Acid pharmacology, Commelina drug effects, Gene Expression Regulation, Plant drug effects, Proton-Translocating ATPases metabolism, Signal Transduction drug effects, Commelina metabolism, Light, Plant Growth Regulators pharmacology, Plant Stomata drug effects
- Abstract
Regulation of stomatal aperture is essential for plant growth and survival in response to environmental stimuli. Opening of stomata induces uptake of CO2 for photosynthesis and transpiration, which enhances uptake of nutrients from roots. Light is the most important stimulus for stomatal opening. Under drought stress, the plant hormone ABA induces stomatal closure to prevent water loss. However, the molecular mechanisms of stomatal movements are not fully understood. In this study, we screened chemical libraries to identify compounds that affect stomatal movements in Commelina benghalensis and characterize the underlying molecular mechanisms. We identified nine stomatal closing compounds (SCL1-SCL9) that suppress light-induced stomatal opening by >50%, and two compounds (temsirolimus and CP-100356) that induce stomatal opening in the dark. Further investigations revealed that SCL1 and SCL2 had no effect on autophosphorylation of phototropin or the activity of the inward-rectifying plasma membrane (PM) K+ channel, KAT1, but suppressed blue light-induced phosphorylation of the penultimate residue, threonine, in PM H+-ATPase, which is a key enzyme for stomatal opening. SCL1 and SCL2 had no effect on ABA-dependent responses, including seed germination and expression of ABA-induced genes. These results suggest that SCL1 and SCL2 suppress light-induced stomatal opening at least in part by inhibiting blue light-induced activation of PM H+-ATPase, but not by the ABA signaling pathway. Interestingly, spraying leaves onto dicot and monocot plants with SCL1 suppressed wilting of leaves, indicating that inhibition of stomatal opening by these compounds confers tolerance to drought stress in plants.
- Published
- 2018
- Full Text
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50. An immune-modulating formula comprising whey peptides and fermented milk improves inflammation-related remote organ injuries in diet-induced acute pancreatitis in mice.
- Author
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Nakamura K, Fukatsu K, Sasayama A, and Yamaji T
- Abstract
It has been demonstrated that an immune-modulating enteral formula enriched with whey peptides and fermented milk (IMF) had anti-inflammatory effects in some experimental models when it was administered before the induction of inflammation. Here, we investigated the anti-inflammatory effects of the IMF administration after the onset of systemic inflammation and investigated whether the IMF could improve the remote organ injuries in an acute pancreatitis (AP) model. Mice were fasted for 12 hours and then fed a choline-deficient and ethionine-supplemented diet (CDE diet) for 24 hours to induce pancreatitis. In experiment 1, the diet was replaced with a control enteral formula, and mice were sacrificed at 24-hour intervals for 96 hours. In experiment 2, mice were randomized into control and IMF groups and received the control formula or the IMF respectively for 72 hr or 96 hr. In experiment 1, pancreatitis was induced by the CDE diet, and inflammatory mediators were elevated for several days. Remote organ injuries such as splenomegaly, hepatomegaly, and elevation of the hepatic enzymes developed. A significant strong positive correlation was observed between plasma MCP-1 and hepatic enzymes. In experiment 2, the IMF significantly improved splenomegaly, hepatomegaly, and the elevation of hepatic enzymes. Plasma MCP-1 levels were significantly lower in the IMF group than in the control group. Nutrition management with the IMF may be useful for alleviating remote organ injuries after AP.
- Published
- 2018
- Full Text
- View/download PDF
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